KR102669561B1 - Aav 벡터 컬럼 정제 방법 - Google Patents

Aav 벡터 컬럼 정제 방법 Download PDF

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KR102669561B1
KR102669561B1 KR1020207002118A KR20207002118A KR102669561B1 KR 102669561 B1 KR102669561 B1 KR 102669561B1 KR 1020207002118 A KR1020207002118 A KR 1020207002118A KR 20207002118 A KR20207002118 A KR 20207002118A KR 102669561 B1 KR102669561 B1 KR 102669561B1
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raav
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KR20200040749A (ko
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영훈 오
광 쿠
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스파크 테라퓨틱스, 인코포레이티드
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/362Cation-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/363Anion-exchange
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
KR1020207002118A 2017-06-30 2018-06-29 Aav 벡터 컬럼 정제 방법 Active KR102669561B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020247016903A KR102879640B1 (ko) 2017-06-30 2018-06-29 Aav 벡터 컬럼 정제 방법

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201762527633P 2017-06-30 2017-06-30
US62/527,633 2017-06-30
US201762531744P 2017-07-12 2017-07-12
US62/531,744 2017-07-12
US201762567905P 2017-10-04 2017-10-04
US62/567,905 2017-10-04
PCT/US2018/040430 WO2019006390A1 (en) 2017-06-30 2018-06-29 AAV VECTOR COLUMN PURIFICATION METHOD

Related Child Applications (1)

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KR1020247016903A Division KR102879640B1 (ko) 2017-06-30 2018-06-29 Aav 벡터 컬럼 정제 방법

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KR20200040749A KR20200040749A (ko) 2020-04-20
KR102669561B1 true KR102669561B1 (ko) 2024-05-24

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KR1020247016903A Active KR102879640B1 (ko) 2017-06-30 2018-06-29 Aav 벡터 컬럼 정제 방법

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US (1) US20210079422A1 (enExample)
EP (1) EP3658250A4 (enExample)
JP (3) JP2020526190A (enExample)
KR (2) KR102669561B1 (enExample)
CN (1) CN111032176A (enExample)
AU (1) AU2018291023B2 (enExample)
CA (1) CA3068622A1 (enExample)
CL (1) CL2019003915A1 (enExample)
CO (1) CO2020000911A2 (enExample)
IL (1) IL271745A (enExample)
MX (2) MX2020000216A (enExample)
MY (1) MY207534A (enExample)
PE (2) PE20251545A1 (enExample)
PH (1) PH12020500044A1 (enExample)
SG (1) SG11201913157RA (enExample)
WO (1) WO2019006390A1 (enExample)

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AU2021262609A1 (en) 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein
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KR20230074703A (ko) * 2020-06-24 2023-05-31 바이오버라티브 테라퓨틱스 인크. 유리 인자 viii을 상기 단백질이 발현되도록 변형된 렌티바이러스 벡터의 제제로부터 제거하는 방법
US20220033782A1 (en) * 2020-07-29 2022-02-03 Pall Corporation Adenovirus-associated viruses separation method
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JP7767029B2 (ja) * 2021-05-28 2025-11-11 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
JP7716233B2 (ja) * 2021-05-28 2025-07-31 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
PE20240359A1 (es) * 2021-06-11 2024-02-27 Spark Therapeutics Inc Metodos de purificacion de columna vectorial aav
AU2022358612A1 (en) * 2021-09-28 2024-04-11 Kashiv Biosciences, Llc An improved process of purification of fusion protein
CN114250222A (zh) * 2021-12-08 2022-03-29 苏州博腾生物制药有限公司 一种从动物组织中提取aav dna的方法
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JP2023141423A (ja) * 2022-03-24 2023-10-05 国立大学法人 東京大学 ウイルスの精製方法
WO2023198685A1 (en) 2022-04-13 2023-10-19 F. Hoffmann-La Roche Ag Method for determining aav genomes
CN119256220A (zh) 2022-05-23 2025-01-03 豪夫迈·罗氏有限公司 用于区分aav颗粒血清型和aav颗粒荷载状态的基于拉曼的方法
EP4532737A1 (en) 2022-06-03 2025-04-09 F. Hoffmann-La Roche AG Method for producing recombinant aav particles
KR20250036743A (ko) 2022-07-14 2025-03-14 에프. 호프만-라 로슈 아게 재조합 aav 입자를 생산하는 방법
JPWO2024024337A1 (enExample) * 2022-07-28 2024-02-01
EP4744752A1 (en) 2022-09-12 2026-05-20 Hoffmann La Roche METHOD FOR SEPARING SOLID AND EMPTY AAV PARTICLES
EP4385618A1 (en) 2022-12-16 2024-06-19 Chiral Technologies Europe SAS Composite material for bioseparations
IL322001A (en) 2023-02-07 2025-09-01 Hoffmann La Roche Method for detecting antibodies to anti-AAV particles
EP4683676A1 (en) 2023-03-21 2026-01-28 F. Hoffmann-La Roche AG Method for the production of recombinant aav particle preparations
KR20250172636A (ko) * 2023-04-12 2025-12-09 씨티바 바이오프로세스 알&디 에이비 크로마토그래피 시스템, 이의 용도, 및 아데노-연관 캡시드를 분리하는 방법
WO2025168663A1 (en) 2024-02-09 2025-08-14 F. Hoffmann-La Roche Ag Method for producing recombinant adeno-associated viral particles
TW202603164A (zh) * 2024-04-07 2026-01-16 美商星火治療公司 用於腺相關病毒殼體變異體純化之組成物及方法
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna
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Publication number Publication date
WO2019006390A1 (en) 2019-01-03
EP3658250A4 (en) 2021-10-27
JP2023029832A (ja) 2023-03-07
JP2025038906A (ja) 2025-03-19
CL2019003915A1 (es) 2020-06-19
PE20251545A1 (es) 2025-06-05
KR102879640B1 (ko) 2025-10-30
MX2024007103A (es) 2024-06-24
MY207534A (en) 2025-03-03
KR20240093848A (ko) 2024-06-24
BR112019028299A2 (pt) 2020-07-14
CO2020000911A2 (es) 2020-06-19
MX2020000216A (es) 2020-09-03
CN111032176A (zh) 2020-04-17
AU2018291023B2 (en) 2023-11-02
EP3658250A1 (en) 2020-06-03
KR20200040749A (ko) 2020-04-20
PH12020500044A1 (en) 2020-09-14
JP2020526190A (ja) 2020-08-31
US20210079422A1 (en) 2021-03-18
SG11201913157RA (en) 2020-01-30
IL271745A (en) 2020-02-27
CA3068622A1 (en) 2019-01-03
RU2020103743A (ru) 2021-07-30
RU2020103743A3 (enExample) 2021-09-30
AU2018291023A1 (en) 2020-02-06
PE20200737A1 (es) 2020-07-23

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