JP2020526190A - Ａａｖベクターのカラム精製方法 - Google Patents

Ａａｖベクターのカラム精製方法 Download PDF

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Publication number
JP2020526190A
JP2020526190A JP2019572175A JP2019572175A JP2020526190A JP 2020526190 A JP2020526190 A JP 2020526190A JP 2019572175 A JP2019572175 A JP 2019572175A JP 2019572175 A JP2019572175 A JP 2019572175A JP 2020526190 A JP2020526190 A JP 2020526190A
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column
column eluate
vector particles
raav vector
lysate
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Pending
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JP2019572175A
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English (en)
Japanese (ja)
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JP2020526190A5 (enExample
Inventor
ヨンフン オウ，
ヨンフン オウ，
グァン チュウ，
グァン チュウ，
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Spark Therapeutics Inc
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Spark Therapeutics Inc
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Publication of JP2020526190A publication Critical patent/JP2020526190A/ja
Publication of JP2020526190A5 publication Critical patent/JP2020526190A5/ja
Priority to JP2022177960A priority Critical patent/JP2023029832A/ja
Priority to JP2024211621A priority patent/JP2025038906A/ja
Pending legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
JP2019572175A 2017-06-30 2018-06-29 Ａａｖベクターのカラム精製方法 Pending JP2020526190A (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2022177960A JP2023029832A (ja) 2017-06-30 2022-11-07 Ａａｖベクターのカラム精製方法
JP2024211621A JP2025038906A (ja) 2017-06-30 2024-12-04 Ａａｖベクターのカラム精製方法

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201762527633P 2017-06-30 2017-06-30
US62/527,633 2017-06-30
US201762531744P 2017-07-12 2017-07-12
US62/531,744 2017-07-12
US201762567905P 2017-10-04 2017-10-04
US62/567,905 2017-10-04
PCT/US2018/040430 WO2019006390A1 (en) 2017-06-30 2018-06-29 AAV VECTOR COLUMN PURIFICATION METHOD

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022177960A Division JP2023029832A (ja) 2017-06-30 2022-11-07 Ａａｖベクターのカラム精製方法

Publications (2)

Publication Number Publication Date
JP2020526190A true JP2020526190A (ja) 2020-08-31
JP2020526190A5 JP2020526190A5 (enExample) 2021-07-26

Family

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JP2019572175A Pending JP2020526190A (ja) 2017-06-30 2018-06-29 Ａａｖベクターのカラム精製方法
JP2022177960A Pending JP2023029832A (ja) 2017-06-30 2022-11-07 Ａａｖベクターのカラム精製方法
JP2024211621A Pending JP2025038906A (ja) 2017-06-30 2024-12-04 Ａａｖベクターのカラム精製方法

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JP2022177960A Pending JP2023029832A (ja) 2017-06-30 2022-11-07 Ａａｖベクターのカラム精製方法
JP2024211621A Pending JP2025038906A (ja) 2017-06-30 2024-12-04 Ａａｖベクターのカラム精製方法

Country Status (16)

Country Link
US (1) US20210079422A1 (enExample)
EP (1) EP3658250A4 (enExample)
JP (3) JP2020526190A (enExample)
KR (2) KR102669561B1 (enExample)
CN (1) CN111032176A (enExample)
AU (1) AU2018291023B2 (enExample)
CA (1) CA3068622A1 (enExample)
CL (1) CL2019003915A1 (enExample)
CO (1) CO2020000911A2 (enExample)
IL (1) IL271745A (enExample)
MX (2) MX2020000216A (enExample)
MY (1) MY207534A (enExample)
PE (2) PE20251545A1 (enExample)
PH (1) PH12020500044A1 (enExample)
SG (1) SG11201913157RA (enExample)
WO (1) WO2019006390A1 (enExample)

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WO2022250035A1 (ja) * 2021-05-28 2022-12-01 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
WO2022250036A1 (ja) * 2021-05-28 2022-12-01 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
WO2023182428A1 (ja) * 2022-03-24 2023-09-28 国立大学法人 東京大学 ウイルスの精製方法
WO2024024337A1 (ja) * 2022-07-28 2024-02-01 株式会社ダイセル 微小有用物質を含む液の精製濃縮装置及びそれを用いた微小有用物質を含む精製濃縮液の製造方法

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US12565645B2 (en) * 2019-04-11 2026-03-03 Regenxbio Inc. Methods of size exclusion chromatography for the characterization of recombinant adeno-associated virus compositions
US12403164B2 (en) 2019-09-30 2025-09-02 Bioverativ Therapeutics Inc. Lentiviral vector formulations
AU2021262609A1 (en) 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein
EP3919613B1 (en) * 2020-06-05 2024-08-07 Sartorius BIA Separations d.o.o. Enhanced purification of adeno-associated virus to more effectively remove contaminating dna
KR20230074703A (ko) * 2020-06-24 2023-05-31 바이오버라티브 테라퓨틱스 인크. 유리 인자 viii을 상기 단백질이 발현되도록 변형된 렌티바이러스 벡터의 제제로부터 제거하는 방법
US20220033782A1 (en) * 2020-07-29 2022-02-03 Pall Corporation Adenovirus-associated viruses separation method
EP4229204A1 (en) 2020-10-15 2023-08-23 F. Hoffmann-La Roche AG Nucleic acid constructs for simultaneous gene activation
KR20230085929A (ko) 2020-10-15 2023-06-14 에프. 호프만-라 로슈 아게 Va rna 전사를 위한 핵산 구조체
PE20240359A1 (es) * 2021-06-11 2024-02-27 Spark Therapeutics Inc Metodos de purificacion de columna vectorial aav
AU2022358612A1 (en) * 2021-09-28 2024-04-11 Kashiv Biosciences, Llc An improved process of purification of fusion protein
CN114250222A (zh) * 2021-12-08 2022-03-29 苏州博腾生物制药有限公司 一种从动物组织中提取aav dna的方法
GB202117844D0 (en) * 2021-12-09 2022-01-26 Oxford Biomedica Ltd Purification method
WO2023198685A1 (en) 2022-04-13 2023-10-19 F. Hoffmann-La Roche Ag Method for determining aav genomes
CN119256220A (zh) 2022-05-23 2025-01-03 豪夫迈·罗氏有限公司 用于区分aav颗粒血清型和aav颗粒荷载状态的基于拉曼的方法
EP4532737A1 (en) 2022-06-03 2025-04-09 F. Hoffmann-La Roche AG Method for producing recombinant aav particles
KR20250036743A (ko) 2022-07-14 2025-03-14 에프. 호프만-라 로슈 아게 재조합 aav 입자를 생산하는 방법
EP4744752A1 (en) 2022-09-12 2026-05-20 Hoffmann La Roche METHOD FOR SEPARING SOLID AND EMPTY AAV PARTICLES
EP4385618A1 (en) 2022-12-16 2024-06-19 Chiral Technologies Europe SAS Composite material for bioseparations
IL322001A (en) 2023-02-07 2025-09-01 Hoffmann La Roche Method for detecting antibodies to anti-AAV particles
EP4683676A1 (en) 2023-03-21 2026-01-28 F. Hoffmann-La Roche AG Method for the production of recombinant aav particle preparations
KR20250172636A (ko) * 2023-04-12 2025-12-09 씨티바 바이오프로세스 알＆디 에이비 크로마토그래피 시스템, 이의 용도, 및 아데노-연관 캡시드를 분리하는 방법
WO2025168663A1 (en) 2024-02-09 2025-08-14 F. Hoffmann-La Roche Ag Method for producing recombinant adeno-associated viral particles
TW202603164A (zh) * 2024-04-07 2026-01-16 美商星火治療公司 用於腺相關病毒殼體變異體純化之組成物及方法
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna
WO2026022064A1 (en) 2024-07-22 2026-01-29 F. Hoffmann-La Roche Ag Novel aav rep orfs and rep polypeptides
WO2026037757A1 (en) 2024-08-13 2026-02-19 F. Hoffmann-La Roche Ag Modified aav particles

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022250035A1 (ja) * 2021-05-28 2022-12-01 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
WO2022250036A1 (ja) * 2021-05-28 2022-12-01 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
JP2022182360A (ja) * 2021-05-28 2022-12-08 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
JP2022182361A (ja) * 2021-05-28 2022-12-08 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
JP7716233B2 (ja) 2021-05-28 2025-07-31 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
JP7767029B2 (ja) 2021-05-28 2025-11-11 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
WO2023182428A1 (ja) * 2022-03-24 2023-09-28 国立大学法人 東京大学 ウイルスの精製方法
JP2023141423A (ja) * 2022-03-24 2023-10-05 国立大学法人 東京大学 ウイルスの精製方法
WO2024024337A1 (ja) * 2022-07-28 2024-02-01 株式会社ダイセル 微小有用物質を含む液の精製濃縮装置及びそれを用いた微小有用物質を含む精製濃縮液の製造方法

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WO2019006390A1 (en) 2019-01-03
EP3658250A4 (en) 2021-10-27
JP2023029832A (ja) 2023-03-07
JP2025038906A (ja) 2025-03-19
CL2019003915A1 (es) 2020-06-19
PE20251545A1 (es) 2025-06-05
KR102879640B1 (ko) 2025-10-30
MX2024007103A (es) 2024-06-24
MY207534A (en) 2025-03-03
KR20240093848A (ko) 2024-06-24
BR112019028299A2 (pt) 2020-07-14
CO2020000911A2 (es) 2020-06-19
MX2020000216A (es) 2020-09-03
CN111032176A (zh) 2020-04-17
AU2018291023B2 (en) 2023-11-02
EP3658250A1 (en) 2020-06-03
KR20200040749A (ko) 2020-04-20
PH12020500044A1 (en) 2020-09-14
US20210079422A1 (en) 2021-03-18
KR102669561B1 (ko) 2024-05-24
SG11201913157RA (en) 2020-01-30
IL271745A (en) 2020-02-27
CA3068622A1 (en) 2019-01-03
RU2020103743A (ru) 2021-07-30
RU2020103743A3 (enExample) 2021-09-30
AU2018291023A1 (en) 2020-02-06
PE20200737A1 (es) 2020-07-23

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