CN111032176A - Aav载体柱纯化方法 - Google Patents

Aav载体柱纯化方法 Download PDF

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Publication number
CN111032176A
CN111032176A CN201880053245.7A CN201880053245A CN111032176A CN 111032176 A CN111032176 A CN 111032176A CN 201880053245 A CN201880053245 A CN 201880053245A CN 111032176 A CN111032176 A CN 111032176A
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China
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column
column eluate
produce
produced
raav
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Pending
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CN201880053245.7A
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English (en)
Chinese (zh)
Inventor
吴永勋
曲光
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Spark Therapeutics Inc
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Spark Therapeutics Inc
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Publication of CN111032176A publication Critical patent/CN111032176A/zh
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
CN201880053245.7A 2017-06-30 2018-06-29 Aav载体柱纯化方法 Pending CN111032176A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201762527633P 2017-06-30 2017-06-30
US62/527,633 2017-06-30
US201762531744P 2017-07-12 2017-07-12
US62/531,744 2017-07-12
US201762567905P 2017-10-04 2017-10-04
US62/567,905 2017-10-04
PCT/US2018/040430 WO2019006390A1 (en) 2017-06-30 2018-06-29 AAV VECTOR COLUMN PURIFICATION METHOD

Publications (1)

Publication Number Publication Date
CN111032176A true CN111032176A (zh) 2020-04-17

Family

ID=64742749

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880053245.7A Pending CN111032176A (zh) 2017-06-30 2018-06-29 Aav载体柱纯化方法

Country Status (16)

Country Link
US (1) US20210079422A1 (enExample)
EP (1) EP3658250A4 (enExample)
JP (3) JP2020526190A (enExample)
KR (2) KR102669561B1 (enExample)
CN (1) CN111032176A (enExample)
AU (1) AU2018291023B2 (enExample)
CA (1) CA3068622A1 (enExample)
CL (1) CL2019003915A1 (enExample)
CO (1) CO2020000911A2 (enExample)
IL (1) IL271745A (enExample)
MX (2) MX2020000216A (enExample)
MY (1) MY207534A (enExample)
PE (2) PE20251545A1 (enExample)
PH (1) PH12020500044A1 (enExample)
SG (1) SG11201913157RA (enExample)
WO (1) WO2019006390A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114058596A (zh) * 2020-07-29 2022-02-18 帕尔公司 腺相关病毒分离方法
CN116033927A (zh) * 2020-06-24 2023-04-28 比奥维拉迪维治疗股份有限公司 从经修饰以表达因子viii的慢病毒载体的制剂中去除游离因子viii的方法

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US12565645B2 (en) * 2019-04-11 2026-03-03 Regenxbio Inc. Methods of size exclusion chromatography for the characterization of recombinant adeno-associated virus compositions
US12403164B2 (en) 2019-09-30 2025-09-02 Bioverativ Therapeutics Inc. Lentiviral vector formulations
AU2021262609A1 (en) 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein
EP3919613B1 (en) * 2020-06-05 2024-08-07 Sartorius BIA Separations d.o.o. Enhanced purification of adeno-associated virus to more effectively remove contaminating dna
EP4229204A1 (en) 2020-10-15 2023-08-23 F. Hoffmann-La Roche AG Nucleic acid constructs for simultaneous gene activation
KR20230085929A (ko) 2020-10-15 2023-06-14 에프. 호프만-라 로슈 아게 Va rna 전사를 위한 핵산 구조체
JP7767029B2 (ja) * 2021-05-28 2025-11-11 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
JP7716233B2 (ja) * 2021-05-28 2025-07-31 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
PE20240359A1 (es) * 2021-06-11 2024-02-27 Spark Therapeutics Inc Metodos de purificacion de columna vectorial aav
AU2022358612A1 (en) * 2021-09-28 2024-04-11 Kashiv Biosciences, Llc An improved process of purification of fusion protein
CN114250222A (zh) * 2021-12-08 2022-03-29 苏州博腾生物制药有限公司 一种从动物组织中提取aav dna的方法
GB202117844D0 (en) * 2021-12-09 2022-01-26 Oxford Biomedica Ltd Purification method
JP2023141423A (ja) * 2022-03-24 2023-10-05 国立大学法人 東京大学 ウイルスの精製方法
WO2023198685A1 (en) 2022-04-13 2023-10-19 F. Hoffmann-La Roche Ag Method for determining aav genomes
CN119256220A (zh) 2022-05-23 2025-01-03 豪夫迈·罗氏有限公司 用于区分aav颗粒血清型和aav颗粒荷载状态的基于拉曼的方法
EP4532737A1 (en) 2022-06-03 2025-04-09 F. Hoffmann-La Roche AG Method for producing recombinant aav particles
KR20250036743A (ko) 2022-07-14 2025-03-14 에프. 호프만-라 로슈 아게 재조합 aav 입자를 생산하는 방법
JPWO2024024337A1 (enExample) * 2022-07-28 2024-02-01
EP4744752A1 (en) 2022-09-12 2026-05-20 Hoffmann La Roche METHOD FOR SEPARING SOLID AND EMPTY AAV PARTICLES
EP4385618A1 (en) 2022-12-16 2024-06-19 Chiral Technologies Europe SAS Composite material for bioseparations
IL322001A (en) 2023-02-07 2025-09-01 Hoffmann La Roche Method for detecting antibodies to anti-AAV particles
EP4683676A1 (en) 2023-03-21 2026-01-28 F. Hoffmann-La Roche AG Method for the production of recombinant aav particle preparations
KR20250172636A (ko) * 2023-04-12 2025-12-09 씨티바 바이오프로세스 알＆디 에이비 크로마토그래피 시스템, 이의 용도, 및 아데노-연관 캡시드를 분리하는 방법
WO2025168663A1 (en) 2024-02-09 2025-08-14 F. Hoffmann-La Roche Ag Method for producing recombinant adeno-associated viral particles
TW202603164A (zh) * 2024-04-07 2026-01-16 美商星火治療公司 用於腺相關病毒殼體變異體純化之組成物及方法
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna
WO2026022064A1 (en) 2024-07-22 2026-01-29 F. Hoffmann-La Roche Ag Novel aav rep orfs and rep polypeptides
WO2026037757A1 (en) 2024-08-13 2026-02-19 F. Hoffmann-La Roche Ag Modified aav particles

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US20150024467A1 (en) * 2009-06-16 2015-01-22 Genzyme Corporation Methods for purification of recombinant aav vectors

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US6593123B1 (en) * 2000-08-07 2003-07-15 Avigen, Inc. Large-scale recombinant adeno-associated virus (rAAV) production and purification
US7261544B2 (en) * 2003-05-21 2007-08-28 Genzyme Corporation Methods for producing preparations of recombinant AAV virions substantially free of empty capsids
MX2018006682A (es) * 2015-12-01 2018-09-26 Spark Therapeutics Inc Metodos escalables para producir vector viral adenoasociado (aav) recombinante en un sistema de cultivo celular en suspension libre de suero adecuado para su uso clinico.
US11015173B2 (en) * 2015-12-11 2021-05-25 The Trustees Of The University Of Pennsylvania Scalable purification method for AAV1
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116033927A (zh) * 2020-06-24 2023-04-28 比奥维拉迪维治疗股份有限公司 从经修饰以表达因子viii的慢病毒载体的制剂中去除游离因子viii的方法
CN114058596A (zh) * 2020-07-29 2022-02-18 帕尔公司 腺相关病毒分离方法

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WO2019006390A1 (en) 2019-01-03
EP3658250A4 (en) 2021-10-27
JP2023029832A (ja) 2023-03-07
JP2025038906A (ja) 2025-03-19
CL2019003915A1 (es) 2020-06-19
PE20251545A1 (es) 2025-06-05
KR102879640B1 (ko) 2025-10-30
MX2024007103A (es) 2024-06-24
MY207534A (en) 2025-03-03
KR20240093848A (ko) 2024-06-24
BR112019028299A2 (pt) 2020-07-14
CO2020000911A2 (es) 2020-06-19
MX2020000216A (es) 2020-09-03
AU2018291023B2 (en) 2023-11-02
EP3658250A1 (en) 2020-06-03
KR20200040749A (ko) 2020-04-20
PH12020500044A1 (en) 2020-09-14
JP2020526190A (ja) 2020-08-31
US20210079422A1 (en) 2021-03-18
KR102669561B1 (ko) 2024-05-24
SG11201913157RA (en) 2020-01-30
IL271745A (en) 2020-02-27
CA3068622A1 (en) 2019-01-03
RU2020103743A (ru) 2021-07-30
RU2020103743A3 (enExample) 2021-09-30
AU2018291023A1 (en) 2020-02-06
PE20200737A1 (es) 2020-07-23

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