PE20240359A1 - Metodos de purificacion de columna vectorial aav - Google Patents

Metodos de purificacion de columna vectorial aav

Info

Publication number
PE20240359A1
PE20240359A1 PE2023003271A PE2023003271A PE20240359A1 PE 20240359 A1 PE20240359 A1 PE 20240359A1 PE 2023003271 A PE2023003271 A PE 2023003271A PE 2023003271 A PE2023003271 A PE 2023003271A PE 20240359 A1 PE20240359 A1 PE 20240359A1
Authority
PE
Peru
Prior art keywords
complete
particles
raav
chromatographic medium
raav particles
Prior art date
Application number
PE2023003271A
Other languages
English (en)
Inventor
Vijesh Kumar
Mi Jin
Ohnmar Khanal
Original Assignee
Spark Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spark Therapeutics Inc filed Critical Spark Therapeutics Inc
Publication of PE20240359A1 publication Critical patent/PE20240359A1/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/422—Displacement mode
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

En el presente documento se describen metodos para la purificacion de particulas de rAAV, particularmente para la purificacion de particulas de rAAV completas que comprende (a) proporcionar una preparacion de rAAV que comprende particulas de rAAV completas y particulas de rAAV no completas; (b) cargr la preparacion de rAAV en un tampon de carga en una columna que comprende un medio cromatografico, en donde las particulas de rAAV completas tienen una mayor afiidad de union al medio cromatografico que las particulas no completas, y la cantidad de particulas de rAAV complatas y particulas no completas que se aplica a la columna supera la capacidad de union del medio cromatografico, de manera que las particulas no completas unidas al medio cromatografico son desplazadas por las particluas de rAAV completas hacia el flujo de la columna; y (c) eluir las particulas completas de rAAV unidas al medio cromatografico con un tampon de elucion para obtener una preparacion purificada.
PE2023003271A 2021-06-11 2022-06-10 Metodos de purificacion de columna vectorial aav PE20240359A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163209680P 2021-06-11 2021-06-11
US202263366094P 2022-06-09 2022-06-09
PCT/US2022/072859 WO2022261663A1 (en) 2021-06-11 2022-06-10 Aav vector column purification methods

Publications (1)

Publication Number Publication Date
PE20240359A1 true PE20240359A1 (es) 2024-02-27

Family

ID=84426294

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2023003271A PE20240359A1 (es) 2021-06-11 2022-06-10 Metodos de purificacion de columna vectorial aav

Country Status (9)

Country Link
EP (1) EP4351755A1 (es)
KR (1) KR20240021231A (es)
AU (1) AU2022289035A1 (es)
BR (1) BR112023025999A2 (es)
CA (1) CA3221540A1 (es)
CO (1) CO2023018191A2 (es)
IL (1) IL309070A (es)
PE (1) PE20240359A1 (es)
WO (1) WO2022261663A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024036251A1 (en) * 2022-08-12 2024-02-15 Ultragenyx Pharmaceutical Inc. Novel anion-exchange chromatography methods for separation of empty from full recombinant adeno-associated virus particles

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI2277996T1 (sl) * 2003-05-21 2014-12-31 Genzyme Corporation Postopki za izdelavo pripravkov rekombinantnih AAV virionov v bistvu brez praznih kapsid
CA3068622A1 (en) * 2017-06-30 2019-01-03 Spark Therapeutics, Inc. Aav vector column purification methods

Also Published As

Publication number Publication date
AU2022289035A1 (en) 2024-01-04
IL309070A (en) 2024-02-01
KR20240021231A (ko) 2024-02-16
BR112023025999A2 (pt) 2024-02-27
CA3221540A1 (en) 2022-12-15
CO2023018191A2 (es) 2024-01-25
EP4351755A1 (en) 2024-04-17
WO2022261663A1 (en) 2022-12-15

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