CL2019003915A1 - Métodos de purificación de columna de vector aav. - Google Patents

Métodos de purificación de columna de vector aav.

Info

Publication number
CL2019003915A1
CL2019003915A1 CL2019003915A CL2019003915A CL2019003915A1 CL 2019003915 A1 CL2019003915 A1 CL 2019003915A1 CL 2019003915 A CL2019003915 A CL 2019003915A CL 2019003915 A CL2019003915 A CL 2019003915A CL 2019003915 A1 CL2019003915 A1 CL 2019003915A1
Authority
CL
Chile
Prior art keywords
chromatography
purification methods
column purification
affinity
aav
Prior art date
Application number
CL2019003915A
Other languages
English (en)
Inventor
Guang Qu
Younghoon Oh
Original Assignee
Spark Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spark Therapeutics Inc filed Critical Spark Therapeutics Inc
Publication of CL2019003915A1 publication Critical patent/CL2019003915A1/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

EN ESTE DOCUMENTO SE DESCRIBEN Y PROPORCIONAN MÉTODOS DE PURIFICACIÓN, PRODUCCIÓN Y FABRICACIÓN DE PARTÍCULAS DE VECTOR VIRAL ADENOASOCIADOS RECOMBINANTE (RAAV). LOS MÉTODOS DE PURIFICACIÓN, PRODUCCIÓN Y FABRICACIÓN ESTABLECIDOS EN EL PRESENTE DOCUMENTO INCLUYEN, POR EJEMPLO, POR LO MENOS 2 ETAPAS DE CROMATOGRAFÍA DE COLUMNA. LAS ETAPAS DE CROMATOGRAFÍA DE COLUMNA INCLUYEN, POR EJEMPLO, CROMATOGRAFÍA DE INTERCAMBIO CATIÓNICO, CROMATOGRAFÍA DE INTERCAMBIO ANIÓNICO, CROMATOGRAFÍA DE EXCLUSIÓN POR TAMAÑO Y/O CROMATOGRAFÍA DE AFINIDAD POR AAV SOLA O EN COMBINACIÓN Y EN CUALQUIER ORDEN.
CL2019003915A 2017-06-30 2019-12-30 Métodos de purificación de columna de vector aav. CL2019003915A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762527633P 2017-06-30 2017-06-30
US201762531744P 2017-07-12 2017-07-12
US201762567905P 2017-10-04 2017-10-04

Publications (1)

Publication Number Publication Date
CL2019003915A1 true CL2019003915A1 (es) 2020-06-19

Family

ID=64742749

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2019003915A CL2019003915A1 (es) 2017-06-30 2019-12-30 Métodos de purificación de columna de vector aav.

Country Status (15)

Country Link
US (1) US20210079422A1 (es)
EP (1) EP3658250A4 (es)
JP (2) JP2020526190A (es)
CN (1) CN111032176A (es)
AU (1) AU2018291023B2 (es)
BR (1) BR112019028299A2 (es)
CA (1) CA3068622A1 (es)
CL (1) CL2019003915A1 (es)
CO (1) CO2020000911A2 (es)
IL (1) IL271745A (es)
MX (1) MX2020000216A (es)
PE (1) PE20200737A1 (es)
PH (1) PH12020500044A1 (es)
SG (1) SG11201913157RA (es)
WO (1) WO2019006390A1 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201508026D0 (en) 2015-05-11 2015-06-24 Ucl Business Plc Capsid
SI3953483T1 (sl) * 2019-04-11 2024-02-29 Regenxbio Inc. Postopki gelske izključitvene kromatografije za karakterizacijo spojin rekombinantnih adeno-povezanih virusov
EP3919613A1 (en) * 2020-06-05 2021-12-08 Bia Separations D.O.O. Enhanced purification of adeno-associated virus to more effectively remove contaminating dna
IL299333A (en) * 2020-06-24 2023-02-01 Bioverativ Therapeutics Inc Methods for the purification of viral vectors
US20220033782A1 (en) * 2020-07-29 2022-02-03 Pall Corporation Adenovirus-associated viruses separation method
AU2021363098A1 (en) 2020-10-15 2023-05-18 F. Hoffmann-La Roche Ag Nucleic acid constructs for simultaneous gene activation
AR123777A1 (es) 2020-10-15 2023-01-11 Hoffmann La Roche Constructos de ácido nucleico para transcripción de arn va
JP2022182360A (ja) * 2021-05-28 2022-12-08 ダイセン・メンブレン・システムズ株式会社 細胞外小胞の分離精製方法
JP2022182361A (ja) * 2021-05-28 2022-12-08 ダイセン・メンブレン・システムズ株式会社 微小有用物質の分離精製方法と分離精製装置
WO2022261663A1 (en) * 2021-06-11 2022-12-15 Spark Therapeutics, Inc. Aav vector column purification methods
WO2023053031A1 (en) * 2021-09-28 2023-04-06 Kashiv Biosciences, Llc An improved process of purification of fusion protein
CN114250222A (zh) * 2021-12-08 2022-03-29 苏州博腾生物制药有限公司 一种从动物组织中提取aav dna的方法
GB202117844D0 (en) * 2021-12-09 2022-01-26 Oxford Biomedica Ltd Purification method
JP2023141423A (ja) * 2022-03-24 2023-10-05 国立大学法人 東京大学 ウイルスの精製方法
WO2023198685A1 (en) 2022-04-13 2023-10-19 F. Hoffmann-La Roche Ag Method for determining aav genomes
WO2023227438A1 (en) 2022-05-23 2023-11-30 F. Hoffmann-La Roche Ag Raman-based method for the differentiation of aav particle serotype and aav particle loading status
WO2023232922A1 (en) 2022-06-03 2023-12-07 F. Hoffmann-La Roche Ag Method for producing recombinant aav particles
WO2024013239A1 (en) 2022-07-14 2024-01-18 F. Hoffmann-La Roche Ag Method for producing recombinant aav particles
WO2024024337A1 (ja) * 2022-07-28 2024-02-01 株式会社ダイセル 微小有用物質を含む液の精製濃縮装置及びそれを用いた微小有用物質を含む精製濃縮液の製造方法
WO2024056561A1 (en) 2022-09-12 2024-03-21 F. Hoffmann-La Roche Ag Method for separating full and empty aav particles

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2302992C (en) * 1997-09-05 2011-11-01 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of recombinant aav vectors
US6593123B1 (en) * 2000-08-07 2003-07-15 Avigen, Inc. Large-scale recombinant adeno-associated virus (rAAV) production and purification
US7261544B2 (en) * 2003-05-21 2007-08-28 Genzyme Corporation Methods for producing preparations of recombinant AAV virions substantially free of empty capsids
MX2011013613A (es) * 2009-06-16 2012-01-19 Genzyme Corp Metodos mejorados para purificacion de vecotres de aav recombinantes.
CA2787827C (en) * 2010-01-28 2020-11-10 The Children's Hospital Of Philadelphia A scalable manufacturing platform for viral vector purification and viral vectors so purified for use in gene therapy
SG11201804400SA (en) * 2015-12-01 2018-06-28 Spark Therapeutics Inc Scalable methods for producing recombinant adeno-associated viral (aav) vector in serum-free suspension cell culture system suitable for clinical use
US11028372B2 (en) * 2015-12-11 2021-06-08 The Trustees Of The University Of Pennsylvania Scalable purification method for AAVRH10
WO2017100674A1 (en) * 2015-12-11 2017-06-15 The Trustees Of The University Of Pennsylvania Scalable purification method for aav1
EP3387117B1 (en) * 2015-12-11 2022-11-23 The Trustees Of The University Of Pennsylvania Scalable purification method for aav8
PT3436051T (pt) * 2016-03-31 2021-11-02 Spark Therapeutics Inc Processo de fabrico de raav com base em coluna totalmente escalável

Also Published As

Publication number Publication date
BR112019028299A2 (pt) 2020-07-14
MX2020000216A (es) 2020-09-03
SG11201913157RA (en) 2020-01-30
IL271745A (en) 2020-02-27
CA3068622A1 (en) 2019-01-03
AU2018291023B2 (en) 2023-11-02
EP3658250A4 (en) 2021-10-27
RU2020103743A3 (es) 2021-09-30
PE20200737A1 (es) 2020-07-23
PH12020500044A1 (en) 2020-09-14
WO2019006390A1 (en) 2019-01-03
AU2018291023A1 (en) 2020-02-06
JP2020526190A (ja) 2020-08-31
CO2020000911A2 (es) 2020-06-19
CN111032176A (zh) 2020-04-17
EP3658250A1 (en) 2020-06-03
US20210079422A1 (en) 2021-03-18
JP2023029832A (ja) 2023-03-07
RU2020103743A (ru) 2021-07-30
KR20200040749A (ko) 2020-04-20

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