AR123777A1 - Constructos de ácido nucleico para transcripción de arn va - Google Patents
Constructos de ácido nucleico para transcripción de arn vaInfo
- Publication number
- AR123777A1 AR123777A1 ARP210102827A ARP210102827A AR123777A1 AR 123777 A1 AR123777 A1 AR 123777A1 AR P210102827 A ARP210102827 A AR P210102827A AR P210102827 A ARP210102827 A AR P210102827A AR 123777 A1 AR123777 A1 AR 123777A1
- Authority
- AR
- Argentina
- Prior art keywords
- adenoviral
- open reading
- nucleic acid
- reading frame
- rna
- Prior art date
Links
- 108091034131 VA RNA Proteins 0.000 title abstract 8
- 108020004707 nucleic acids Proteins 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 230000035897 transcription Effects 0.000 title 1
- 238000013518 transcription Methods 0.000 title 1
- 108700026244 Open Reading Frames Proteins 0.000 abstract 8
- 108020004414 DNA Proteins 0.000 abstract 3
- 239000002245 particle Substances 0.000 abstract 3
- 108091026890 Coding region Proteins 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 210000004962 mammalian cell Anatomy 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 abstract 1
- 102100021244 Integral membrane protein GPR180 Human genes 0.000 abstract 1
- 108700019146 Transgenes Proteins 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14123—Virus like particles [VLP]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14151—Methods of production or purification of viral material
- C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
Abstract
Reivindicación 1: Un ácido nucleico de ARN VA adenoviral que comprende, en dirección 5 a 3 un promotor de ARN U6 humano y una secuencia codificante de ARN VA adenoviral de SEQ ID Nº 38. Reivindicación 2: Un ácido nucleico de ARN VA adenoviral que comprende, en dirección 5 a 3 un promotor inducible y una secuencia codificante de ARN VA adenoviral de SEQ ID Nº 38. Reivindicación 3: Un ADN que comprende un ácido nucleico de ARN VA adenoviral de acuerdo con una cualquiera de las reivindicaciones 1 ó 2, y un elemento de ADN que comprende un marco de lectura abierto E1A y un marco de lectura abierto E1B; o un marco de lectura abierto E2A y un marco de lectura abierto E4 o E4orf6; o un marco de lectura abierto rep y un marco de lectura abierto cap. Reivindicación 4: Una célula de mamífero o de insecto que comprende un ácido nucleico de ARN VA adenoviral de acuerdo con una cualquiera de las reivindicaciones 1 a 2 o un ADN de acuerdo con la reivindicación 3. Reivindicación 5: Un método para producir partículas de virus adenoasociado recombinante que comprende: proporcionar una célula de mamífero, cultivada en suspensión, que comprende un casete de expresión transgénica interespaciado entre dos ITR de AAV; marcos de lectura abiertos que codifican las proteínas E1A, E1B, E2A, E4 o E4orf6 adenovirales; un ácido nucleico de ARN VA adenoviral de acuerdo con una cualquiera de las reivindicaciones 1 a 2; marcos de lectura abiertos que codifican proteínas Rep / Cap adenoasociadas; cultivar la célula de mamífero; y aislar las partículas de rAAV de la célula o el medio de cultivo y, de esta manera, producir partículas de rAAV.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP20202010 | 2020-10-15 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR123777A1 true AR123777A1 (es) | 2023-01-11 |
Family
ID=72964437
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP210102827A AR123777A1 (es) | 2020-10-15 | 2021-10-13 | Constructos de ácido nucleico para transcripción de arn va |
Country Status (12)
Country | Link |
---|---|
US (1) | US20220135954A1 (es) |
EP (1) | EP4229205A1 (es) |
JP (1) | JP2023546116A (es) |
KR (1) | KR20230085929A (es) |
CN (1) | CN116406425A (es) |
AR (1) | AR123777A1 (es) |
AU (1) | AU2021360197A1 (es) |
CA (1) | CA3197730A1 (es) |
IL (1) | IL302046A (es) |
MX (1) | MX2023004052A (es) |
TW (1) | TW202223094A (es) |
WO (1) | WO2022079083A1 (es) |
Family Cites Families (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4797368A (en) | 1985-03-15 | 1989-01-10 | The United States Of America As Represented By The Department Of Health And Human Services | Adeno-associated virus as eukaryotic expression vector |
US5139941A (en) | 1985-10-31 | 1992-08-18 | University Of Florida Research Foundation, Inc. | AAV transduction vectors |
US6093699A (en) | 1987-07-09 | 2000-07-25 | The University Of Manitoba | Method for gene therapy involving suppression of an immune response |
DK0452457T3 (da) | 1989-11-03 | 1998-03-02 | Univ Vanderbilt | Fremgangsmåde til in vivo fjernelse af funktionelle fremmede gener |
US5279833A (en) | 1990-04-04 | 1994-01-18 | Yale University | Liposomal transfection of nucleic acids into animal cells |
WO1991018088A1 (en) | 1990-05-23 | 1991-11-28 | The United States Of America, Represented By The Secretary, United States Department Of Commerce | Adeno-associated virus (aav)-based eucaryotic vectors |
US5173414A (en) | 1990-10-30 | 1992-12-22 | Applied Immune Sciences, Inc. | Production of recombinant adeno-associated virus vectors |
EP0557459B1 (en) | 1990-11-13 | 1997-10-22 | Immunex Corporation | Bifunctional selectable fusion genes |
US5587308A (en) | 1992-06-02 | 1996-12-24 | The United States Of America As Represented By The Department Of Health & Human Services | Modified adeno-associated virus vector capable of expression from a novel promoter |
IL105914A0 (en) | 1992-06-04 | 1993-10-20 | Univ California | Methods and compositions for in vivo gene therapy |
WO1994017810A1 (en) | 1993-02-12 | 1994-08-18 | The Wistar Institute Of Anatomy And Biology | Recombinant cytomegalovirus vaccine |
CA2160583A1 (en) | 1993-04-16 | 1994-10-27 | Stanley A. Plotkin | Recombinant cytomegalovirus vaccine |
US5670361A (en) | 1993-05-17 | 1997-09-23 | The Regents Of The University Of California | HIV-specific ribozymes |
EP0804590A1 (en) | 1993-05-21 | 1997-11-05 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
US5998205A (en) | 1994-11-28 | 1999-12-07 | Genetic Therapy, Inc. | Vectors for tissue-specific replication |
WO1996026742A1 (en) | 1995-02-28 | 1996-09-06 | The Regents Of The University Of California | Gene transfer-mediated angiogenesis therapy |
US6001650A (en) | 1995-08-03 | 1999-12-14 | Avigen, Inc. | High-efficiency wild-type-free AAV helper functions |
EP1983057A3 (en) | 1995-09-08 | 2009-01-07 | Genzyme Corporation | Improved AAV vectors for gene therapy |
US6004797A (en) | 1995-11-09 | 1999-12-21 | Avigen, Inc. | Adenovirus helper-free recombinant AAV Virion production |
WO1997032481A1 (en) | 1996-03-07 | 1997-09-12 | The Regents Of The University Of California | Helper-free, totally defective adenovirus for gene therapy |
DE19955558C2 (de) | 1999-11-18 | 2003-03-20 | Stefan Kochanek | Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren |
AU2001257611A1 (en) | 2000-04-28 | 2001-11-12 | Avigen, Inc. | Polynucleotides for use in recombinant adeno-associated virus virion production |
US6593123B1 (en) | 2000-08-07 | 2003-07-15 | Avigen, Inc. | Large-scale recombinant adeno-associated virus (rAAV) production and purification |
EP1546397A4 (en) | 2002-09-27 | 2007-10-31 | Cold Spring Harbor Lab | CELL-BASED RNA INTERFERENCE AND METHODS AND COMPOSITIONS RELATING THERETO |
FR2852021B1 (fr) * | 2003-03-04 | 2007-12-07 | Methodes et outils pour le criblage d'arn actifs in cellulo | |
EP2277996B1 (en) | 2003-05-21 | 2014-09-03 | Genzyme Corporation | Methods for producing preparations of recombinant AAV virions substantially free of empty capsids |
DE102005054628A1 (de) | 2005-11-16 | 2007-05-24 | Cevec Pharmaceuticals Gmbh | Verfahren zur Herstellung von permanenten humanen Zelllinien |
ES2680915T3 (es) | 2010-01-28 | 2018-09-11 | The Children's Hospital Of Philadelphia | Plataforma de fabricación escalable para la purificación de vectores virales y vectores virales purificados de este modo para su utilización en terapia génica |
US20130058871A1 (en) | 2011-07-28 | 2013-03-07 | Howard Hughes Medical Institute | Method and system for mapping synaptic connectivity using light microscopy |
EP3795581A3 (en) | 2011-08-24 | 2021-06-09 | The Board of Trustees of the Leland Stanford Junior University | New avv capsid proteins for nucleic acid transfer |
HUE054087T2 (hu) | 2012-04-18 | 2021-09-28 | Childrens Hospital Philadelphia | Készítmények és eljárások nagy hatékonyságú géntranszferre AAV kapszidvariánsok alkalmazásával |
PT3024498T (pt) | 2013-07-22 | 2020-03-06 | Childrens Hospital Philadelphia | Variante aav e composições, métodos e usos para transferência genética em células, órgãos e tecidos |
EP3039129B1 (en) * | 2013-08-30 | 2018-06-27 | Amgen Inc. | High titer recombinant aav vector production in adherent and suspension cells |
EP3204050A4 (en) | 2014-10-09 | 2018-04-04 | The Regents of The University of California | Targeted disruption of a csf1-dap12 pathway member gene for the treatment of neuropathic pain |
AU2016362317B2 (en) | 2015-12-01 | 2023-03-16 | Spark Therapeutics, Inc. | Scalable methods for producing recombinant Adeno-Associated Viral (AAV) vector in serum-free suspension cell culture system suitable for clinical use |
JP2020507331A (ja) * | 2017-02-17 | 2020-03-12 | ロンザ リミテッドLonza Limited | アデノ随伴ウイルスを生産するための哺乳動物細胞 |
AU2018281306A1 (en) | 2017-06-07 | 2020-01-16 | Spark Therapeutics, Inc. | Enhancing agents for improved cell transfection and/or rAAV vector production |
PE20200737A1 (es) | 2017-06-30 | 2020-07-23 | Spark Therapeutics Inc | Metodos de purificacion de columna de vector aav |
US20210002669A1 (en) | 2017-12-22 | 2021-01-07 | Genentech, Inc. | Targeted integration of nucleic acids |
SG11202106217TA (en) * | 2018-12-21 | 2021-07-29 | Lonza Walkersville Inc | Adeno-associated virus (aav) producer cell line and related methods |
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2021
- 2021-10-13 MX MX2023004052A patent/MX2023004052A/es unknown
- 2021-10-13 CN CN202180069666.0A patent/CN116406425A/zh active Pending
- 2021-10-13 US US17/500,778 patent/US20220135954A1/en active Pending
- 2021-10-13 AR ARP210102827A patent/AR123777A1/es unknown
- 2021-10-13 KR KR1020237016221A patent/KR20230085929A/ko unknown
- 2021-10-13 EP EP21786513.8A patent/EP4229205A1/en active Pending
- 2021-10-13 CA CA3197730A patent/CA3197730A1/en active Pending
- 2021-10-13 JP JP2023523028A patent/JP2023546116A/ja active Pending
- 2021-10-13 WO PCT/EP2021/078269 patent/WO2022079083A1/en active Application Filing
- 2021-10-13 AU AU2021360197A patent/AU2021360197A1/en active Pending
- 2021-10-13 IL IL302046A patent/IL302046A/en unknown
- 2021-10-14 TW TW110138078A patent/TW202223094A/zh unknown
Also Published As
Publication number | Publication date |
---|---|
JP2023546116A (ja) | 2023-11-01 |
CN116406425A (zh) | 2023-07-07 |
TW202223094A (zh) | 2022-06-16 |
WO2022079083A1 (en) | 2022-04-21 |
MX2023004052A (es) | 2023-05-03 |
EP4229205A1 (en) | 2023-08-23 |
AU2021360197A1 (en) | 2023-05-18 |
US20220135954A1 (en) | 2022-05-05 |
IL302046A (en) | 2023-06-01 |
AU2021360197A9 (en) | 2023-07-13 |
CA3197730A1 (en) | 2022-04-21 |
KR20230085929A (ko) | 2023-06-14 |
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