KR102639658B1 - 3-하이드록시프로피온산의 2단계 제조 방법 - Google Patents

3-하이드록시프로피온산의 2단계 제조 방법 Download PDF

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KR102639658B1
KR102639658B1 KR1020210100741A KR20210100741A KR102639658B1 KR 102639658 B1 KR102639658 B1 KR 102639658B1 KR 1020210100741 A KR1020210100741 A KR 1020210100741A KR 20210100741 A KR20210100741 A KR 20210100741A KR 102639658 B1 KR102639658 B1 KR 102639658B1
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hydroxypropionic acid
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production
culture
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KR20220015996A (ko
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김재형
강동균
이경묵
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주식회사 엘지화학
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
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    • C12N9/88—Lyases (4.)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
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    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01001—Formaldehyde dehydrogenase (glutathione) (1.2.1.1)
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    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
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    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/22—Klebsiella
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    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01003—Aldehyde dehydrogenase (NAD+) (1.2.1.3)
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    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01004—Aldehyde dehydrogenase (NADP+) (1.2.1.4)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01005—Aldehyde dehydrogenase [NAD(P)+] (1.2.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/0103—Glycerol dehydratase (4.2.1.30)

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020210100741A 2020-07-31 2021-07-30 3-하이드록시프로피온산의 2단계 제조 방법 Active KR102639658B1 (ko)

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KR20200096238 2020-07-31
KR1020200096238 2020-07-31

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KR20220015996A KR20220015996A (ko) 2022-02-08
KR102639658B1 true KR102639658B1 (ko) 2024-02-22

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US (1) US20230265466A1 (https=)
EP (1) EP4190907A4 (https=)
JP (2) JP2023531938A (https=)
KR (1) KR102639658B1 (https=)
CN (1) CN115956123A (https=)
WO (1) WO2022025710A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20250114925A (ko) * 2024-01-22 2025-07-29 주식회사 엘지화학 3-하이드록시프로피온산의 생산 방법
CN121488048A (zh) * 2024-01-31 2026-02-06 株式会社Lg化学 用于生产3-羟基丙酸的方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60214873A (ja) * 1984-04-10 1985-10-28 Kirin Brewery Co Ltd 酒類の製造法
BR0008355A (pt) * 1999-08-30 2002-07-16 Wisconsin Alumni Res Found Produção de ácido 3-hidroxipropionico em organismos recombinantes
EP2960325B1 (en) * 2000-01-28 2017-09-27 DSM IP Assets B.V. Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
CN102317436B (zh) * 2008-07-23 2014-06-25 Opx生物工艺学公司 用于增加微生物对3-羟基丙酸(3-hp)的耐受性以及增加3-羟基丙酸产量的方法、系统和组合物
KR101189187B1 (ko) * 2009-03-12 2012-10-10 한국생명공학연구원 글리세롤 산화대사경로를 차단시킨 재조합 균주를 이용한 １、３―프로판디올의 제조방법
KR101090362B1 (ko) * 2009-10-29 2011-12-07 한국생명공학연구원 글리세롤로부터 3-하이드록시프로피온산을 생산하는 새로운 방법
US9090918B2 (en) * 2010-11-22 2015-07-28 Novozymes A/A Compositions and methods for 3-hydroxypropionic acid production
KR20120099315A (ko) * 2011-01-26 2012-09-10 삼성전자주식회사 3-하이드록시프로피온산 및 1,3-프로판디올 동시 생산용 재조합미생물
JP2013071898A (ja) * 2011-09-27 2013-04-22 Nippon Shokubai Co Ltd ３−ヒドロキシプロピオン酸類の製造方法
CN102559790B (zh) * 2012-02-07 2014-03-12 清华大学 一种提高产油微生物发酵生产微生物油脂的方法
KR101437042B1 (ko) * 2012-12-28 2014-09-02 삼성전자 주식회사 포도당 및 글리세롤을 이용한 3-히드록시프로피온산의 생산방법
KR101483012B1 (ko) * 2013-01-22 2015-01-19 서울대학교산학협력단 재조합 대장균을 이용하여 3-히드록시프로피온산을 고수율로 생산하는 방법
KR20150011111A (ko) * 2013-07-22 2015-01-30 삼성종합화학주식회사 대장균 돌연변이체 및 이를 사용한 유기산 합성방법
CN103789388B (zh) * 2014-02-19 2016-05-18 江南大学 一种以甘油为原料发酵-生物催化耦合合成体系生产3-羟基丙酸的方法
CN104774790B (zh) * 2015-04-03 2020-11-06 江南大学 一种高效发酵生产l-丙氨酸的大肠杆菌
FI126781B (en) * 2015-05-25 2017-05-31 Neste Corp Methods for the continuous production of products from microorganisms
CN108291231B (zh) * 2015-06-11 2022-04-08 纳路Ic有限公司 由3-羟基丙酸诱导表达的启动子系统及用其生物生产3-羟基丙酸的方法
KR101760340B1 (ko) * 2016-01-07 2017-07-21 포항공과대학교 산학협력단 3-히드록시프로피온산을 생산하는 재조합 미생물 및 이를 이용한 3-히드록시프로피온산의 생산방법
EP3358015A1 (en) 2017-02-03 2018-08-08 Eligo Bioscience Optimized vector for delivery in microbial populations
KR102103408B1 (ko) * 2018-01-16 2020-04-23 한국과학기술원 1,3-프로판디올 생성능을 가지는 변이미생물 및 이를 이용한 1,3-pdo의 제조방법

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Publication number Publication date
JP2023531938A (ja) 2023-07-26
WO2022025710A1 (ko) 2022-02-03
KR20220015996A (ko) 2022-02-08
CN115956123A (zh) 2023-04-11
JP7852981B2 (ja) 2026-04-28
EP4190907A1 (en) 2023-06-07
EP4190907A4 (en) 2023-12-06
US20230265466A1 (en) 2023-08-24
JP2024163267A (ja) 2024-11-21

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