KR102631090B1 - 미세유체 장치의 사용 방법 - Google Patents

미세유체 장치의 사용 방법 Download PDF

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Publication number
KR102631090B1
KR102631090B1 KR1020227029286A KR20227029286A KR102631090B1 KR 102631090 B1 KR102631090 B1 KR 102631090B1 KR 1020227029286 A KR1020227029286 A KR 1020227029286A KR 20227029286 A KR20227029286 A KR 20227029286A KR 102631090 B1 KR102631090 B1 KR 102631090B1
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microfluidic device
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KR20220122799A (ko
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주-성 헝
앤드류 자약
메간 듀크
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콤비네티 인코포레이티드
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micromachines (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
KR1020227029286A 2016-04-04 2017-04-04 미세유체 장치의 사용 방법 Active KR102631090B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020247001750A KR102893830B1 (ko) 2016-04-04 2017-04-04 핵산 정량을 위한 미세유체 사이퍼닝 어레이

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201662317993P 2016-04-04 2016-04-04
US62/317,993 2016-04-04
US15/363,896 US9845499B2 (en) 2016-04-04 2016-11-29 Microfluidic siphoning array for nucleic acid quantification
US15/363,896 2016-11-29
PCT/US2017/025873 WO2017176699A1 (en) 2016-04-04 2017-04-04 Microfluidic siphoning array for nucleic acid quantification
KR1020187031928A KR102438312B1 (ko) 2016-04-04 2017-04-04 핵산 정량을 위한 미세유체 사이퍼닝 어레이

Related Parent Applications (1)

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KR1020187031928A Division KR102438312B1 (ko) 2016-04-04 2017-04-04 핵산 정량을 위한 미세유체 사이퍼닝 어레이

Related Child Applications (1)

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KR1020247001750A Division KR102893830B1 (ko) 2016-04-04 2017-04-04 핵산 정량을 위한 미세유체 사이퍼닝 어레이

Publications (2)

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KR20220122799A KR20220122799A (ko) 2022-09-02
KR102631090B1 true KR102631090B1 (ko) 2024-01-29

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KR1020227029286A Active KR102631090B1 (ko) 2016-04-04 2017-04-04 미세유체 장치의 사용 방법
KR1020247001750A Active KR102893830B1 (ko) 2016-04-04 2017-04-04 핵산 정량을 위한 미세유체 사이퍼닝 어레이
KR1020187031928A Active KR102438312B1 (ko) 2016-04-04 2017-04-04 핵산 정량을 위한 미세유체 사이퍼닝 어레이
KR1020257039814A Pending KR20260003197A (ko) 2016-04-04 2017-04-04 핵산 정량을 위한 미세유체 사이퍼닝 어레이

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KR1020187031928A Active KR102438312B1 (ko) 2016-04-04 2017-04-04 핵산 정량을 위한 미세유체 사이퍼닝 어레이
KR1020257039814A Pending KR20260003197A (ko) 2016-04-04 2017-04-04 핵산 정량을 위한 미세유체 사이퍼닝 어레이

Country Status (8)

Country Link
US (1) US9845499B2 (enExample)
EP (2) EP3439779B1 (enExample)
JP (2) JP6970448B2 (enExample)
KR (4) KR102631090B1 (enExample)
CN (2) CN115232707A (enExample)
AU (3) AU2017246827B2 (enExample)
CA (1) CA3019385A1 (enExample)
WO (1) WO2017176699A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9845499B2 (en) * 2016-04-04 2017-12-19 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
US11285478B2 (en) 2016-04-04 2022-03-29 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
EP3984642B1 (en) 2016-11-17 2023-09-06 Combinati Incorporated Methods and systems for nucleic acid analysis and quantification
WO2019010587A1 (en) * 2017-07-14 2019-01-17 The Governing Council Of The University Of Toronto MICROFLUIDIC PLATFORM FOR THE RAPID PRODUCTION OF ORGANOIDS / SPHEREOIDS FOR SCREENING COMPOUNDS
EP3774052A4 (en) * 2018-04-04 2022-01-05 Combinati Incorporated MICROFLUIDIC SUCTION ARRANGEMENT FOR NUCLEIC ACID QUANTIFICATION
CN113423503A (zh) * 2018-12-10 2021-09-21 康比纳提公司 用于样品数字化的微流体阵列
JP7825558B2 (ja) * 2020-01-24 2026-03-06 コンビナティ インコーポレイテッド マイクロ流体デバイス製造のための方法およびシステム
CA3195362A1 (en) * 2020-09-28 2022-03-31 Combinati Incorporated Devices and methods for sample processing
CN114644976A (zh) * 2020-12-18 2022-06-21 湖南乐准智芯生物科技有限公司 一种微反应室阵列结构及进样方法
EP4271518A1 (en) * 2020-12-29 2023-11-08 Combinati Incorporated Devices and methods for sample partitioning
US20240375102A1 (en) 2021-09-14 2024-11-14 Combinati Incorporated Microfluidic device and method for processing biological samples
CN115093962B (zh) * 2022-08-24 2023-03-24 翊新诊断技术(苏州)有限公司 基于柔性薄膜的微流控芯片及其在核酸扩增中的应用
US20240198336A1 (en) 2022-12-19 2024-06-20 Anchorline Biolabs, Inc. Capillary partitioning microfluidics

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004532003A (ja) * 2001-01-29 2004-10-21 ジェンセット ソシエテ アノニム マイクロリアクター内の連続流において生化学的プロトコルを実施する方法
US20050009101A1 (en) 2001-05-17 2005-01-13 Motorola, Inc. Microfluidic devices comprising biochannels
US20090250347A1 (en) 2008-04-03 2009-10-08 Protea Biosciences, Inc. Microfluidic devices & processes for electrokinetic transport
US20140323358A1 (en) * 2003-09-19 2014-10-30 Applied Biosystems, Llc Vacuum Assist for a Microplate
KR101486413B1 (ko) 2014-08-06 2015-01-26 경희대학교 산학협력단 마이크로 포스트를 이용한 미세유동 칩 및 그 제조방법

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US6361958B1 (en) * 1999-11-12 2002-03-26 Motorola, Inc. Biochannel assay for hybridization with biomaterial
US6569674B1 (en) * 1999-12-15 2003-05-27 Amersham Biosciences Ab Method and apparatus for performing biological reactions on a substrate surface
US6875619B2 (en) * 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
JP2003517591A (ja) * 1999-12-09 2003-05-27 モトローラ・インコーポレイテッド 分析試料の反応を行うための多層微量流体デバイス
JP2002018271A (ja) 2000-07-05 2002-01-22 Kawamura Inst Of Chem Res 微小ケミカルデバイス
JP5695287B2 (ja) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー 微小流体の核酸解析
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
JP2006512092A (ja) 2002-12-30 2006-04-13 ザ・リージェンツ・オブ・ジ・ユニバーシティ・オブ・カリフォルニア 病原体の検出および分析のための方法および装置
WO2005080606A1 (en) * 2004-02-18 2005-09-01 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
JP2005329333A (ja) * 2004-05-20 2005-12-02 Japan Science & Technology Agency マイクロ脱気デバイス
US20070026426A1 (en) 2005-04-26 2007-02-01 Applera Corporation System for genetic surveillance and analysis
KR100785016B1 (ko) * 2006-05-22 2007-12-12 삼성전자주식회사 단일 마이크로 챔버에서 핵산의 농축 및 증폭을 수행하는방법 및 장치
GB2445738A (en) * 2007-01-16 2008-07-23 Lab901 Ltd Microfluidic device
JP2009236555A (ja) * 2008-03-26 2009-10-15 Shimadzu Corp 流体デバイス及びその製造方法
KR20120063162A (ko) 2010-12-07 2012-06-15 삼성전자주식회사 유전자 분석 장치 및 이를 이용한 유전자 분석 방법
EP2697648B1 (en) * 2011-04-13 2018-08-22 Akonni Biosystems, Inc. Microarray based sample detection system
WO2013089887A2 (en) * 2011-09-30 2013-06-20 The Regents Of The University Of California Microfluidic devices and methods for assaying a fluid sample using the same
EP2814942B1 (en) 2012-02-13 2024-07-03 Neumodx Molecular, Inc. Microfluidic cartridge for processing and detecting nucleic acids
ITTO20120703A1 (it) 2012-08-03 2014-02-04 Biomerieux Sa Dispositivo microfluidico monouso, cartuccia includente il dispositivo microfluidico, apparecchio per eseguire una amplificazione di acido nucleico, metodo di fabbricazione del dispositivo microfluidico, e metodo di uso del dispositivo microfluidico
DE102013205412A1 (de) 2013-03-27 2014-10-02 Robert Bosch Gmbh Normal-geschlossenes Ventil für mikrofluidische Bauteile aus einem polymeren Schichtsystem sowie Verfahren
ES2756124T3 (es) 2013-12-06 2020-04-27 Ortho Clinical Diagnostics Inc Dispositivo de ensayo que tiene un puerto de lavado
WO2015085171A1 (en) 2013-12-06 2015-06-11 Adventist Health Systems/Sunbelt, Inc. Role of mcp-1 in relapse of acute myeloid leukemia after hematopoietic stem cell transplantation
KR101562318B1 (ko) * 2014-02-10 2015-10-22 나노바이오시스 주식회사 미세유체 칩 및 이를 이용한 실시간 분석 장치
CN104946510B (zh) * 2014-03-31 2017-06-06 博奥生物集团有限公司 集核酸扩增和微阵列检测于一体的微流控装置
CN104877905A (zh) * 2015-05-04 2015-09-02 清华大学深圳研究生院 细胞体外共培养微流控芯片、系统及方法
CN104894106A (zh) * 2015-05-23 2015-09-09 浙江大学 高集成度等距等分核酸扩增微流控芯片及应用
US9845499B2 (en) * 2016-04-04 2017-12-19 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004532003A (ja) * 2001-01-29 2004-10-21 ジェンセット ソシエテ アノニム マイクロリアクター内の連続流において生化学的プロトコルを実施する方法
US20050009101A1 (en) 2001-05-17 2005-01-13 Motorola, Inc. Microfluidic devices comprising biochannels
US20140323358A1 (en) * 2003-09-19 2014-10-30 Applied Biosystems, Llc Vacuum Assist for a Microplate
US20090250347A1 (en) 2008-04-03 2009-10-08 Protea Biosciences, Inc. Microfluidic devices & processes for electrokinetic transport
KR101486413B1 (ko) 2014-08-06 2015-01-26 경희대학교 산학협력단 마이크로 포스트를 이용한 미세유동 칩 및 그 제조방법

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