KR102626671B1 - 세포외 공간으로부터 표적 진핵 세포의 세포질 및/또는 핵으로 폴리펩타이드 카고를 전달하기 위한 합리적으로-설계된 합성 펩타이드 셔틀제, 이의 용도, 이와 관련된 방법 및 키트 - Google Patents

세포외 공간으로부터 표적 진핵 세포의 세포질 및/또는 핵으로 폴리펩타이드 카고를 전달하기 위한 합리적으로-설계된 합성 펩타이드 셔틀제, 이의 용도, 이와 관련된 방법 및 키트 Download PDF

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KR102626671B1
KR102626671B1 KR1020197013023A KR20197013023A KR102626671B1 KR 102626671 B1 KR102626671 B1 KR 102626671B1 KR 1020197013023 A KR1020197013023 A KR 1020197013023A KR 20197013023 A KR20197013023 A KR 20197013023A KR 102626671 B1 KR102626671 B1 KR 102626671B1
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peptide
cell
cells
cargo
gfp
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KR20190072559A (ko
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토마스 델'쥬디쎄
쟌-파스칼 레쁘티-스토프파에스
데이비드 과이
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펠단 바이오 인코포레이티드
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KR1020197013023A 2016-10-12 2017-10-11 세포외 공간으로부터 표적 진핵 세포의 세포질 및/또는 핵으로 폴리펩타이드 카고를 전달하기 위한 합리적으로-설계된 합성 펩타이드 셔틀제, 이의 용도, 이와 관련된 방법 및 키트 Active KR102626671B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662407232P 2016-10-12 2016-10-12
US62/407,232 2016-10-12
US201762535015P 2017-07-20 2017-07-20
US62/535,015 2017-07-20
US15/666,139 2017-08-01
US15/666,139 US9982267B2 (en) 2016-10-12 2017-08-01 Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same
PCT/CA2017/051205 WO2018068135A1 (en) 2016-10-12 2017-10-11 Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same

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KR20190072559A KR20190072559A (ko) 2019-06-25
KR102626671B1 true KR102626671B1 (ko) 2024-01-18

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US (1) US12286632B2 (https=)
EP (2) EP4556567A3 (https=)
JP (3) JP7177047B2 (https=)
KR (1) KR102626671B1 (https=)
CN (2) CN110291100B (https=)
AU (2) AU2017341736B2 (https=)
CA (1) CA3038839A1 (https=)
IL (1) IL265920B2 (https=)
WO (1) WO2018068135A1 (https=)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2981716C (en) 2015-04-10 2022-04-12 Feldan Bio Inc. Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same
US11629170B2 (en) 2016-10-12 2023-04-18 Feldan Bio Inc. Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same
CN110291100B (zh) 2016-10-12 2024-06-11 费尔丹生物公司 用于将多肽负荷从细胞外空间递送至靶真核细胞的胞质溶胶和/或细胞核的合理设计的合成肽穿梭剂，其用途、与其相关的方法和试剂盒
WO2018232356A1 (en) 2017-06-15 2018-12-20 The Regents Of The University Of California Targeted non-viral dna insertions
JP7101419B2 (ja) 2017-10-27 2022-07-15 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 内因性ｔ細胞受容体の標的置換
US11732009B2 (en) * 2018-06-08 2023-08-22 Glympse Bio, Inc. Activity sensor with tunable analyte
US11028425B2 (en) 2018-06-08 2021-06-08 Glympse Bio, Inc. Diagnosis and monitoring of liver disease
CN109232717B (zh) * 2018-08-31 2021-08-20 东北农业大学 一种针对革兰氏阴性菌靶向抗菌肽及制作方法与应用
CN109232719B (zh) * 2018-09-21 2021-06-29 中国科学院理化技术研究所 一种pH响应的抗菌肽及其制备方法和应用
CN113166209B (zh) * 2018-10-08 2025-01-07 弗里堡大学阿道夫梅克尔研究所 成孔肽的基于寡核苷酸的调节以增加孔尺寸、膜亲和性、稳定性和抗菌活性
MX2021006208A (es) 2018-11-28 2021-10-01 Univ Texas Edición por multiplexación del genoma de células inmunitarias para mejorar la funcionalidad y resistencia al entorno supresor.
PE20211959A1 (es) * 2019-02-15 2021-09-30 Editas Medicine Inc Celulas asesinas naturales (nk) modificadas para inmunoterapia
US12338483B2 (en) 2019-02-28 2025-06-24 Georgia Tech Research Corporation Compositions and methods for logic-gated profiling of biologic activity
WO2020198151A1 (en) * 2019-03-22 2020-10-01 Spotlight Therapeutics Targeted active gene editing agent and methods of use
CA3040645A1 (en) * 2019-04-18 2020-10-18 Feldan Bio Inc. Peptide-based non-proteinaceous cargo delivery
WO2021050601A1 (en) * 2019-09-09 2021-03-18 Scribe Therapeutics Inc. Compositions and methods for use in immunotherapy
WO2021152402A1 (en) * 2020-01-29 2021-08-05 Jenthera Therapeutics Inc. Nuclease-scaffold composition delivery platform
CN111518168B (zh) * 2020-03-30 2022-02-08 东北农业大学 一种衍生自肉食杆菌素的抗菌肽及其制备方法和应用
CN115397843A (zh) * 2020-04-15 2022-11-25 杭州卫本医药科技有限公司 用于筛选药物的靶标多肽和筛选方法
CN115698036B (zh) * 2020-05-28 2026-04-21 米托奎斯特有限公司 新型抗炎肽及其用途
JP2023542867A (ja) 2020-09-11 2023-10-12 グリンプス バイオ， インコーポレイテッド 疾患の検出／診断、病期分類、モニタリング及び処置のための生体外でのプロテアーゼ活性検出
JP2023545546A (ja) * 2020-10-18 2023-10-30 フェルダン・バイオ・インコーポレーテッド 遺伝子発現調節のための非アニオン性ポリヌクレオチド類似体のペプチドに基づく導入
AU2021366976A1 (en) * 2020-10-22 2023-06-08 Feldan Bio Inc. Shuttle agent peptides of minimal length and variants thereof adapted for transduction of cas9-rnp and other nucleoprotein cargos
WO2022120423A1 (en) * 2020-12-08 2022-06-16 James Cook University Peptides and uses thereof
MX2023009816A (es) * 2021-02-24 2024-03-25 Univ Jagiellonski Una jaula de proteínas artificial que comprende una carga de huésped encapsulada en la misma.
JP2024511537A (ja) * 2021-03-29 2024-03-13 フェルダン・バイオ・インコーポレーテッド 細胞内カーゴ送達のための合成ペプチドシャトル媒介物バイオコンジュゲート
CN113214355B (zh) * 2021-04-09 2022-02-25 东北农业大学 一种专杀真菌的抗菌肽gl4w及其制备方法和应用
CN114177306B (zh) * 2021-07-16 2024-01-30 吉林医药学院 一种iNGR/R9双重修饰的阿霉素靶向脂质体及抗肿瘤活性评价
JP2024537991A (ja) 2021-10-14 2024-10-18 アーセナル バイオサイエンシズ インコーポレイテッド 共発現されるｓｈＲＮＡと論理ゲートシステムとを有する免疫細胞
US20230158136A1 (en) * 2021-11-24 2023-05-25 Versitech Limited Viral nucleic acid molecules, and compositions and methods of use thereof
CN114716569B (zh) * 2022-04-13 2023-11-10 浙江大学 一种携带目标蛋白自主进入真核细胞的重组蛋白、重组表达载体和重组菌及应用
CN116178519B (zh) * 2022-08-09 2025-04-22 成都医学院 一种蜂毒肽糖肽及其制备方法和用途
EP4651880A1 (en) * 2023-01-17 2025-11-26 Research Institute at Nationwide Children's Hospital Methods of targeting glycogen synthase kinase 3 beta in nk cells
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CN115974972B (zh) * 2023-02-07 2023-08-29 安徽农业大学 一种防治猕猴桃溃疡病菌的抗菌肽、制备方法及其应用
CN116375805B (zh) * 2023-03-27 2023-09-08 中山大学附属口腔医院 一种兼具抗菌和成骨活性的多肽、其制剂及应用
WO2024219474A1 (ja) * 2023-04-21 2024-10-24 株式会社Logomix Ｋｉｒ遺伝子クラスター領域、ｌｉｌｒ遺伝子クラスター領域およびｋｌｒ遺伝子クラスター領域のいずれか１以上の領域において欠失を有する細胞およびその製造方法
WO2025106604A1 (en) * 2023-11-14 2025-05-22 The University Of North Carolina At Chapel Hill Compositions and methods relating to antiviral therapeutics
CN120271677A (zh) * 2025-06-06 2025-07-08 中国医学科学院病原生物学研究所 一种细胞新型毒力因子的功能鉴定及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016161516A1 (en) 2015-04-10 2016-10-13 Feldan Bio Inc. Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same
WO2017175072A1 (en) 2016-04-08 2017-10-12 Feldan Bio Inc. Peptide shuttle based gene disruption

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030104622A1 (en) * 1999-09-01 2003-06-05 Robbins Paul D. Identification of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, DNA and viruses
FR2849603B1 (fr) 2003-01-07 2006-09-08 Centre Nat Rech Scient Composition pour le transport intracellulaire de macromolecules ou particules biologiques
US7084248B2 (en) 2004-07-14 2006-08-01 Gene Tools, Llc Peptide composition and method for delivering substances into the cytosol of cells
EP1969000A2 (en) 2005-12-06 2008-09-17 Centre National de la Recherche Scientifique Cell penetrating peptides for intracellular delivery of molecules
US7579318B2 (en) 2005-12-06 2009-08-25 Centre De La Recherche De La Scientifique Cell penetrating peptides for intracellular delivery of molecules
EP2837636B1 (en) * 2007-01-29 2018-04-11 Procell Therapeutics Inc. Novel macromolecule transduction domains and methods for identification and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
ES2794599T3 (es) * 2013-09-10 2020-11-18 Texas A & M Univ Sys Composiciones y métodos para la distribución de moléculas en células vivas
KR20160063349A (ko) * 2013-10-17 2016-06-03 서울대학교산학협력단 알파나선형 세포 투과 펩타이드 다합체，이의 제조방법 및 그 용도
JP7103750B2 (ja) 2013-12-12 2022-07-20 ザ・ブロード・インスティテュート・インコーポレイテッド ゲノム編集のためのＣＲＩＳＰＲ－Ｃａｓ系及び組成物の送達、使用及び治療適用
US20160304893A1 (en) * 2013-12-13 2016-10-20 Cellectis Cas9 nuclease platform for microalgae genome engineering
WO2016052442A1 (ja) 2014-09-29 2016-04-07 国立大学法人京都大学 細胞質送達ペプチド
WO2017049407A1 (en) 2015-09-23 2017-03-30 UNIVERSITé LAVAL Modification of the dystrophin gene and uses thereof
CA3037635A1 (en) * 2016-09-23 2018-03-29 Universite Laval Methods of modifying the dystrophin gene and restoring dystrophin expression and uses thereof
US9982267B2 (en) 2016-10-12 2018-05-29 Feldan Bio Inc. Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same
CN110291100B (zh) 2016-10-12 2024-06-11 费尔丹生物公司 用于将多肽负荷从细胞外空间递送至靶真核细胞的胞质溶胶和/或细胞核的合理设计的合成肽穿梭剂，其用途、与其相关的方法和试剂盒
US11629170B2 (en) 2016-10-12 2023-04-18 Feldan Bio Inc. Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same
EP3597223A1 (en) 2018-07-17 2020-01-22 Centre National De La Recherche Scientifique Peptides for use as cell-penetrating peptides
CA3040645A1 (en) 2019-04-18 2020-10-18 Feldan Bio Inc. Peptide-based non-proteinaceous cargo delivery
JP2023545546A (ja) 2020-10-18 2023-10-30 フェルダン・バイオ・インコーポレーテッド 遺伝子発現調節のための非アニオン性ポリヌクレオチド類似体のペプチドに基づく導入
AU2021366976A1 (en) 2020-10-22 2023-06-08 Feldan Bio Inc. Shuttle agent peptides of minimal length and variants thereof adapted for transduction of cas9-rnp and other nucleoprotein cargos
JP2024511537A (ja) 2021-03-29 2024-03-13 フェルダン・バイオ・インコーポレーテッド 細胞内カーゴ送達のための合成ペプチドシャトル媒介物バイオコンジュゲート

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016161516A1 (en) 2015-04-10 2016-10-13 Feldan Bio Inc. Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same
WO2017175072A1 (en) 2016-04-08 2017-10-12 Feldan Bio Inc. Peptide shuttle based gene disruption

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Arch Pharm Res.,39(9):1266-1274(2016.7.5.)
J Controlled Release.,163:293-303(2012.)

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