KR102626671B1 - 세포외 공간으로부터 표적 진핵 세포의 세포질 및/또는 핵으로 폴리펩타이드 카고를 전달하기 위한 합리적으로-설계된 합성 펩타이드 셔틀제, 이의 용도, 이와 관련된 방법 및 키트 - Google Patents
세포외 공간으로부터 표적 진핵 세포의 세포질 및/또는 핵으로 폴리펩타이드 카고를 전달하기 위한 합리적으로-설계된 합성 펩타이드 셔틀제, 이의 용도, 이와 관련된 방법 및 키트 Download PDFInfo
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
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- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
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- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
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- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
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- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
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- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
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- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
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Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662407232P | 2016-10-12 | 2016-10-12 | |
| US62/407,232 | 2016-10-12 | ||
| US201762535015P | 2017-07-20 | 2017-07-20 | |
| US62/535,015 | 2017-07-20 | ||
| US15/666,139 US9982267B2 (en) | 2016-10-12 | 2017-08-01 | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
| US15/666,139 | 2017-08-01 | ||
| PCT/CA2017/051205 WO2018068135A1 (en) | 2016-10-12 | 2017-10-11 | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20190072559A KR20190072559A (ko) | 2019-06-25 |
| KR102626671B1 true KR102626671B1 (ko) | 2024-01-18 |
Family
ID=61905066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020197013023A Active KR102626671B1 (ko) | 2016-10-12 | 2017-10-11 | 세포외 공간으로부터 표적 진핵 세포의 세포질 및/또는 핵으로 폴리펩타이드 카고를 전달하기 위한 합리적으로-설계된 합성 펩타이드 셔틀제, 이의 용도, 이와 관련된 방법 및 키트 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US12286632B2 (enExample) |
| EP (2) | EP3526235A4 (enExample) |
| JP (3) | JP7177047B2 (enExample) |
| KR (1) | KR102626671B1 (enExample) |
| CN (2) | CN110291100B (enExample) |
| AU (2) | AU2017341736B2 (enExample) |
| CA (1) | CA3038839A1 (enExample) |
| IL (1) | IL265920B2 (enExample) |
| WO (1) | WO2018068135A1 (enExample) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6956705B2 (ja) | 2015-04-10 | 2021-11-02 | フェルダン・バイオ・インコーポレーテッド | 標的真核細胞のサイトゾルへのポリペプチドカーゴの形質導入効率を改善するためのポリペプチドベースのシャトル剤、その使用、それと関連する方法及びキット |
| US11629170B2 (en) | 2016-10-12 | 2023-04-18 | Feldan Bio Inc. | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
| AU2017341736B2 (en) * | 2016-10-12 | 2022-09-08 | Feldan Bio Inc. | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
| MX2019015188A (es) | 2017-06-15 | 2020-08-03 | Univ California | Inserciones de adn no virales orientadas. |
| AU2018355587B2 (en) | 2017-10-27 | 2023-02-02 | The Regents Of The University Of California | Targeted replacement of endogenous T cell receptors |
| US11028425B2 (en) | 2018-06-08 | 2021-06-08 | Glympse Bio, Inc. | Diagnosis and monitoring of liver disease |
| US11732009B2 (en) | 2018-06-08 | 2023-08-22 | Glympse Bio, Inc. | Activity sensor with tunable analyte |
| CN109232717B (zh) * | 2018-08-31 | 2021-08-20 | 东北农业大学 | 一种针对革兰氏阴性菌靶向抗菌肽及制作方法与应用 |
| CN109232719B (zh) * | 2018-09-21 | 2021-06-29 | 中国科学院理化技术研究所 | 一种pH响应的抗菌肽及其制备方法和应用 |
| US12281178B2 (en) | 2018-10-08 | 2025-04-22 | Adolphe Merkle Institute, University Of Fribourg | Tuning of pore-forming peptides for increasing pore size, membrane affinity, stability, and antimicrobial activity |
| BR112021010297A2 (pt) * | 2018-11-28 | 2021-08-24 | Board Of Regents, The University Of Texas System | Edição de genomas multiplexadores de células imunes para aumentar a funcionalidade e resistência ao ambiente supressivo |
| MX2021009742A (es) * | 2019-02-15 | 2021-12-10 | Editas Medicine Inc | Celulas asesinas naturales (nk) modificadas para inmunoterapia. |
| CA3131900A1 (en) | 2019-02-28 | 2020-09-24 | Georgia Tech Research Corporation | Compositions and methods for logic-gated profiling of biologic activity |
| CA3134500A1 (en) * | 2019-03-22 | 2020-10-01 | Spotlight Therapeutics | Targeted active gene editing agent and methods of use |
| CA3040645A1 (en) * | 2019-04-18 | 2020-10-18 | Feldan Bio Inc. | Peptide-based non-proteinaceous cargo delivery |
| EP4028523A1 (en) * | 2019-09-09 | 2022-07-20 | Scribe Therapeutics Inc. | Compositions and methods for use in immunotherapy |
| EP4097237A4 (en) * | 2020-01-29 | 2023-08-16 | Jenthera Therapeutics Inc. | NUCLEASE SCAFFOLD COMPOSITION DELIVERY PLATFORM |
| CN111518168B (zh) * | 2020-03-30 | 2022-02-08 | 东北农业大学 | 一种衍生自肉食杆菌素的抗菌肽及其制备方法和应用 |
| WO2021208787A1 (zh) * | 2020-04-15 | 2021-10-21 | 徐荣臻 | 用于筛选药物的靶标多肽和筛选方法 |
| CN115698036A (zh) * | 2020-05-28 | 2023-02-03 | 米托奎斯特有限公司 | 新型抗炎肽及其用途 |
| CA3194956A1 (en) | 2020-09-11 | 2022-03-17 | Glympse Bio, Inc. | Ex vivo protease activity detection for disease detection/diagnostic, staging, monitoring and treatment |
| CA3195442A1 (en) * | 2020-10-18 | 2022-04-21 | Jean-Pascal LEPETIT-STOFFAES | Peptide-based transduction of non-anionic polynucleotide analogs for gene expression modulation |
| KR20230088828A (ko) * | 2020-10-22 | 2023-06-20 | 펠단 바이오 인코포레이티드 | Cas9-rnp 및 기타 핵단백질 카고의 형질도입을 위해 적합화된 최소 길이의 셔틀제 펩타이드 및 그의 변이체 |
| WO2022120423A1 (en) * | 2020-12-08 | 2022-06-16 | James Cook University | Peptides and uses thereof |
| IL307352A (en) * | 2021-03-29 | 2023-11-01 | Feldan Bio Inc | Biological conjugates of a synthetic peptide transporter for intracellular cargo transport |
| CN113214355B (zh) * | 2021-04-09 | 2022-02-25 | 东北农业大学 | 一种专杀真菌的抗菌肽gl4w及其制备方法和应用 |
| CN114177306B (zh) * | 2021-07-16 | 2024-01-30 | 吉林医药学院 | 一种iNGR/R9双重修饰的阿霉素靶向脂质体及抗肿瘤活性评价 |
| EP4416292A2 (en) | 2021-10-14 | 2024-08-21 | Arsenal Biosciences, Inc. | Immune cells having co-expressed shrnas and logic gate systems |
| US20230158136A1 (en) * | 2021-11-24 | 2023-05-25 | Versitech Limited | Viral nucleic acid molecules, and compositions and methods of use thereof |
| CN114716569B (zh) * | 2022-04-13 | 2023-11-10 | 浙江大学 | 一种携带目标蛋白自主进入真核细胞的重组蛋白、重组表达载体和重组菌及应用 |
| CN116178519B (zh) * | 2022-08-09 | 2025-04-22 | 成都医学院 | 一种蜂毒肽糖肽及其制备方法和用途 |
| EP4651880A1 (en) * | 2023-01-17 | 2025-11-26 | Research Institute at Nationwide Children's Hospital | Methods of targeting glycogen synthase kinase 3 beta in nk cells |
| WO2024157895A1 (ja) | 2023-01-27 | 2024-08-02 | 東レ株式会社 | ポリアミド複合断面繊維、ポリアミドマルチフィラメント及びポリアミド複合断面仮撚加工糸 |
| CN115974972B (zh) * | 2023-02-07 | 2023-08-29 | 安徽农业大学 | 一种防治猕猴桃溃疡病菌的抗菌肽、制备方法及其应用 |
| CN116375805B (zh) * | 2023-03-27 | 2023-09-08 | 中山大学附属口腔医院 | 一种兼具抗菌和成骨活性的多肽、其制剂及应用 |
| WO2024219474A1 (ja) * | 2023-04-21 | 2024-10-24 | 株式会社Logomix | Kir遺伝子クラスター領域、lilr遺伝子クラスター領域およびklr遺伝子クラスター領域のいずれか1以上の領域において欠失を有する細胞およびその製造方法 |
| WO2025106604A1 (en) * | 2023-11-14 | 2025-05-22 | The University Of North Carolina At Chapel Hill | Compositions and methods relating to antiviral therapeutics |
| CN120271677A (zh) * | 2025-06-06 | 2025-07-08 | 中国医学科学院病原生物学研究所 | 一种细胞新型毒力因子的功能鉴定及其应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016161516A1 (en) | 2015-04-10 | 2016-10-13 | Feldan Bio Inc. | Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same |
| WO2017175072A1 (en) | 2016-04-08 | 2017-10-12 | Feldan Bio Inc. | Peptide shuttle based gene disruption |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030104622A1 (en) * | 1999-09-01 | 2003-06-05 | Robbins Paul D. | Identification of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, DNA and viruses |
| FR2849603B1 (fr) | 2003-01-07 | 2006-09-08 | Centre Nat Rech Scient | Composition pour le transport intracellulaire de macromolecules ou particules biologiques |
| US7084248B2 (en) | 2004-07-14 | 2006-08-01 | Gene Tools, Llc | Peptide composition and method for delivering substances into the cytosol of cells |
| US7579318B2 (en) | 2005-12-06 | 2009-08-25 | Centre De La Recherche De La Scientifique | Cell penetrating peptides for intracellular delivery of molecules |
| WO2007069090A2 (en) | 2005-12-06 | 2007-06-21 | Centre National De La Recherche Scientifique | Cell penetrating peptides for intracellular delivery of molecules |
| JP5518488B2 (ja) * | 2007-01-29 | 2014-06-11 | プロセル セラピューティックス インコーポレーティッド | 巨大分子伝達ドメインならびにその同定方法および使用 |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| ES2794599T3 (es) * | 2013-09-10 | 2020-11-18 | Texas A & M Univ Sys | Composiciones y métodos para la distribución de moléculas en células vivas |
| JP2016539922A (ja) * | 2013-10-17 | 2016-12-22 | ソウル ナショナル ユニバーシティ アールアンドディービー ファウンデーション | 細胞透過性αヘリックスペプチド多量体、これの製造方法およびその用途 |
| WO2015089462A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for genome editing |
| CA2933134A1 (en) * | 2013-12-13 | 2015-06-18 | Cellectis | Cas9 nuclease platform for microalgae genome engineering |
| WO2016052442A1 (ja) | 2014-09-29 | 2016-04-07 | 国立大学法人京都大学 | 細胞質送達ペプチド |
| CA2996982A1 (en) | 2015-09-23 | 2017-03-30 | Universite Laval | Modification of the dystrophin gene and uses thereof |
| WO2018053632A1 (en) * | 2016-09-23 | 2018-03-29 | UNIVERSITé LAVAL | Methods of modifying the dystrophin gene and restoring dystrophin expression and uses thereof |
| US9982267B2 (en) | 2016-10-12 | 2018-05-29 | Feldan Bio Inc. | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
| AU2017341736B2 (en) | 2016-10-12 | 2022-09-08 | Feldan Bio Inc. | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
| US11629170B2 (en) | 2016-10-12 | 2023-04-18 | Feldan Bio Inc. | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
| EP3597223A1 (en) | 2018-07-17 | 2020-01-22 | Centre National De La Recherche Scientifique | Peptides for use as cell-penetrating peptides |
| CA3040645A1 (en) | 2019-04-18 | 2020-10-18 | Feldan Bio Inc. | Peptide-based non-proteinaceous cargo delivery |
| CA3195442A1 (en) | 2020-10-18 | 2022-04-21 | Jean-Pascal LEPETIT-STOFFAES | Peptide-based transduction of non-anionic polynucleotide analogs for gene expression modulation |
| KR20230088828A (ko) | 2020-10-22 | 2023-06-20 | 펠단 바이오 인코포레이티드 | Cas9-rnp 및 기타 핵단백질 카고의 형질도입을 위해 적합화된 최소 길이의 셔틀제 펩타이드 및 그의 변이체 |
| IL307352A (en) | 2021-03-29 | 2023-11-01 | Feldan Bio Inc | Biological conjugates of a synthetic peptide transporter for intracellular cargo transport |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016161516A1 (en) | 2015-04-10 | 2016-10-13 | Feldan Bio Inc. | Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same |
| WO2017175072A1 (en) | 2016-04-08 | 2017-10-12 | Feldan Bio Inc. | Peptide shuttle based gene disruption |
Non-Patent Citations (2)
| Title |
|---|
| Arch Pharm Res.,39(9):1266-1274(2016.7.5.) |
| J Controlled Release.,163:293-303(2012.) |
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| KR20190072559A (ko) | 2019-06-25 |
| CA3038839A1 (en) | 2018-04-19 |
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