KR102596697B1 - Cosmetic composition containing Orostachys japonicus extract as an active ingredient and method for manufacturing cosmetic composition - Google Patents

Abstract

The present invention relates to a cosmetic composition containing rock pine extract as an active ingredient. The cosmetic composition is grown and harvested by planting rock brush seedlings, diluting chitosan oligosaccharide in water at a weight ratio of 1:100 to 1:1,000, and then applying foliar application at intervals of several days, and using rock brush extract extracted from the harvested rock brush as an active ingredient. It is characterized by containing.
This cosmetic composition contains as an active ingredient an extract with improved effectiveness compared to the conventional rock pine extract, and promotes skin whitening, moisturizing, elasticity, and regeneration of dead skin cells, maintaining moisture, relieving tightness, dryness, etc., and improving skin whitening and elasticity. It has an enhancing effect, so it can be very suitable and useful as an anti-aging cosmetic composition.

Description

Cosmetic composition containing Orostachys japonicus extract as an active ingredient and method for manufacturing cosmetic composition}

The present invention relates to a cosmetic composition containing a rock pine extract as an active ingredient. More specifically, the present invention relates to a cosmetic composition containing a rock pine extract as an active ingredient. In more detail, various extracts of rock pine grown in eco-friendly organic farming using chitosan oligosaccharide are prepared according to the harvest time and extraction solvent and contained as an active ingredient. relates to an anti-aging cosmetic composition that is excellent for skin whitening, moisturizing and regeneration.

Human skin originates from the ectoderm and mesoderm during development, and its thickness varies depending on the part of our body. The skin is largely divided into two layers: the epidermis layer, which is exposed to the outside, and the dermis layer, which is the layer immediately below. The layers of the palms and soles, which experience a lot of friction, are thicker than other areas because they have a transparent layer on the epidermis. And the epidermis layer of the face is the thinnest, and the lips in particular have the thinnest epidermis layer. The outermost layer of most epidermis layers is composed of dead cells called the stratum corneum, which is involved in defense mechanisms from the outside and inside.

In other words, it has been reported that healthy people have a high concentration of natural moisturizing factors in keratinocytes, which helps the skin's ability to retain moisture related to moisturizing, and because some amino acids are water-soluble, they effectively bind with moisture and prevent skin from drying out. (J. Invest. Dermatol. 54, 24-31, 1970).

In general, skin aging is caused by inflammation caused by cytokines produced in skin cells due to exposure to ultraviolet rays, damage to the epidermal basement membrane, changes in skin color, misuse of various medicines, dry skin, exposure to chemicals, and use of cosmetics.

Free radicals are one of the causes of the above-mentioned conditions caused by ultraviolet rays and respiration, and once generated, free radicals are known to affect blood vessel cells and cause many problems.

In addition, in modern society, human skin is more likely to be exposed to various chemical compounds, and various skin problems occur, such as exposure to external environmental pollution due to changes in living environment, personal stress from daily life, and skin changes due to aging. The frequency is increasing.

Previously, moisturizers were used as one of the methods to resolve the above-mentioned skin troubles. Moisturizers with moisture-absorbing properties or occlusive moisturizers that prevent moisture evaporation were used to ultimately increase moisture retention in the stratum corneum. come.

The above moisturizers mainly include substances such as glycerin, polyethylene glycol, 1,3-butylene glycol, sorbitol, propylene glycol, and sodium 2-pyrrolidone-5-carboxylate, but when applied to the skin, they are not sticky or sticky. The downside is that it feels damp.

In addition, lipid components such as ceramide, essential fatty acids, and lipid complexes have been used as occlusive moisturizers (J. Invest. Dermatol. (5), 731-740, 1994), but it is difficult to maintain the stability of the emulsion formulation, so it is not transparent. It has the disadvantage of being unsuitable for manufacturing gel-type products.

However, recently, moisturizers extracted from natural products are being explored instead of the various chemical moisturizers or occlusive moisturizers mentioned above, and technology for extracting their active ingredients has been developed, increasing their availability. As one of these potential plants, rock brush is rapidly emerging.

Orostachys japonica is a medicinal plant belonging to the Crassulaceae family and is currently an edible food plant (designated in 2013). It is a perennial herbaceous succulent that mainly grows in the mountains and rocks of Korea. The outpost grows to about 20 to 40 cm. The growing environment is that it grows on sunny rocks or tiles around houses, so it is also called 'Wasong (瓦松)'. Many leaves are attached to the main stem, and the ends are sharp like thorns. The flowers are white and bloom from the bottom of the stem and gradually rise upward. When flower stalks appear in the fall (from mid-September), all the leaves, which were dense as they rise from the bottom to the top, move up along the stem and become loose. When flowers bloom and seeds set, the leaves die. Rock brush is a succulent plant. Its roots can rot in places with too much moisture, so it grows well in well-drained soil.

The above-mentioned rock brush has effects such as fever reduction, hemostasis, swelling, and healing. According to Donguibogam, it is used to treat diseases such as sclerosis, hepatitis, eczema, dysentery diarrhea, hemorrhoids, malignant boils, and burns. When applied to a boil, it has a great effect in sucking out pus. also. Apply it to wounds bitten by insects or poisonous snakes as an antidote. Recently, various studies have shown that it has anti-cancer properties.

Rock pine is included among native and medicinal herbs, and contains a variety of functional ingredients that exhibit various pharmacological effects. These ingredients may act alone, or the efficacy through pharmacological action appears through the interaction of the contained ingredients. . According to existing studies, Baeksol contains a lot of flavonoids and polyphenols among the functional ingredients. It has been reported that their main action is excellent antioxidant effect (study on dietary hyperlipidemia (Wonkwang University College of Pharmacy, Hyeon-joo Jeong, 2009), study on antibacterial activity of extracts from different parts of Rock Pine (Pukyong National University, Department of Food Engineering, Dong-hyeon Ahn, 2009), Apoptosis-inducing effect on HL60 leukemia cells (Woosuk University, Department of Food Science and Technology, Oh Chan-ho, 2009), diabetic blood sugar-lowering effect from a complex of herbal medicine and rock sol (Gyeongsang National University, Seong Nakju, 2013), anticancer effect on human prostate cancer cells (Sunchon National University, Food) Studies have been conducted in the Department of Nutrition, Seo Kwon-il, 2014).

Patents related to cosmetics using rock brush as described above include Korean Patent Application No. 10-2015-0169277, “Composition for improving wrinkles and promoting skin damage recovery containing rock brush, trillium chinensis, and hot weather extract” and Korea Patent Application No. 10-2015-0169277 No. 10-2015-0058271, “A composition for improving wrinkles and promoting skin damage recovery containing extracts of Rock pine and Trifolium vulgaris,” is disclosed, and the contents include prevention of wrinkles or extracts of turmeric extract, and extracts of turmeric extract. It relates to cosmetic compositions for improvement, and cosmetic or health food compositions for preventing or promoting skin damage.

Cosmetic compositions containing the above-mentioned Baeksol extract are composed by further enhancing the functionality of the Bamangospermia extract and the extracts of Trifolium vulgaris or Daewigi extract. However, the manufacturing process using such complex extracts is complicated and the stability of the cosmetic composition is complicated. Problems may arise.

Republic of Korea Patent Application No. 10-2015-0169277 “Composition for improving wrinkles and promoting skin damage recovery containing extracts of rock brush, trillium chinensis, and hot weather” Republic of Korea Patent Application No. 10-2015-0058271 “Composition for improving wrinkles and promoting skin damage recovery containing extracts of Bawisul and Cypress chinensis”

Accordingly, the present invention was invented in consideration of the above circumstances, and aims to provide a cosmetic composition with significantly improved effects through a Bavarian pine extract, which has improved effects such as skin whitening, moisturizing, elasticity, and skin regeneration compared to a conventional Baja pine extract. There is a purpose.

In order to achieve the above technical problem, the present invention is to plant rock brush seedlings, dilute chitosan oligosaccharide in water at a weight ratio of 1:100 to 1:1,000, cultivate and harvest by foliar application at intervals of several days, and harvest the rock brush. It is characterized by containing extracted rock pine extract as an active ingredient.

In addition, the harvested rock brush is characterized in that it is harvested before flower stalks emerge.

In addition, the rock pine extract is characterized in that the extraction solvent is water or ethanol.

In addition, the above-mentioned rock pine extract is characterized in that the extraction solvent is ethanol heated to 50-70°C.

A method for manufacturing a cosmetic composition according to another embodiment of the present invention includes planting rock brush seedlings; Diluting chitosan oligosaccharide in water at a weight ratio of 1:100 to 1:1,000 to the rock brush seedlings and then fertilizing the leaves at intervals of several days; Harvesting the cultivated rock brush; Obtaining rock brush extract using harvested rock brush; Preparing a cosmetic composition containing the rock pine extract; Includes.

In addition, the step of harvesting the rock brush is characterized in that it is harvested before the flower stalks of the rock brush emerge.

The cosmetic composition according to the present invention contains as an active ingredient an extract with improved effectiveness compared to the conventional Rock pine extract, and promotes skin whitening, moisturizing, elasticity, and regeneration of the skin's dead skin cells, maintaining moisture, relieving tightness, dryness, etc., and improving skin whitening and It has the effect of increasing elasticity, so it can be very suitable and useful as an anti-aging cosmetic composition.

Hereinafter, the structure and operation of a preferred embodiment of the present invention will be described in detail with reference to the attached drawings. Here, in adding reference numerals to components in each drawing, it should be noted that identical components are indicated with the same reference numerals as much as possible, even if they are shown in different drawings.

The inventor of the present invention plants rock brush seedlings, dilutes chitosan oligosaccharides (chitooligosaccharides) in water at a weight ratio of 1:100 to 1:1,000, and then plants them at intervals of several days, preferably 10 to 20 days (for example, 15 days). ) Organic cultivation of rock pine was able to be completed by foliar application at intervals. Foliar fertilization refers to a method of supplying liquid fertilizer directly to the leaves of plants. Chitosan is obtained by deacetylating the shells of crustaceans (crabs, shrimp, etc.) and the bones of molluscs (squid, cuttlefish, etc.) by pulverizing, deproteinizing, and desalting chitin. This chitosan is treated with enzymes to reduce the number of sugars from 2 to 2. The 10 obtained were chitosan oligosaccharides.

When rock pine extract produced by this cultivation method is extracted and cosmetic compositions are manufactured using the rock pine extract, skin whitening, moisturizing, elasticity, and skin regeneration are achieved more than when using rock pine extract produced through conventional cultivation. The present invention was completed by confirming that such effects were improved.

In the cosmetic composition manufacturing method, planting rock brush seedlings; Diluting chitosan oligosaccharide in water at a weight ratio of 1:100 to 1:1,000 to the rock brush seedlings and then fertilizing the leaves at intervals of several days; Harvesting the cultivated rock brush; Obtaining rock brush extract using harvested rock brush; Preparing a cosmetic composition containing the rock pine extract; Includes.

In addition, while cultivating rock pine seedlings, they were harvested in the early and late stages of flower stalk emergence, and then an extract of rock pine was prepared to confirm the effects of skin whitening, moisturizing, elasticity, and skin regeneration, and the skin whitening and skin regeneration benefits were further improved from the rock pine extract in the early stage of flower stalk emergence. Effects such as moisturizing, elasticity, and skin regeneration were confirmed. Preferably, chitosan oligosaccharides are diluted in water and grown organically through foliar fertilization, and a cosmetic composition is provided that includes an extract extracted by harvesting the flower stalks of the rock brush before emergence as an active ingredient. The flower stalks of the rock pine contain a lot of moisture before sprouting, so it is believed to have excellent skin moisturizing and soothing effects. After the flower stalks of the rock brush are sprouted, more polyphenols and flavonoids, which are sulfated substances, are released.

The extraction method from the rock pine extract according to the present invention is not particularly limited as long as it is known in the art, and is preferably extracted using water or ethanol as an extraction solvent. When extracting harvested rock pine with water, everything from cold immersion to hot water extraction can be used, and when extracting with ethanol, all extractions under various ethanol concentration, temperature, and pressure conditions can be used.

Examples of the rock pine extract according to the present invention are as follows.

(a) Organic rock brush is harvested and washed by season, and (b) water is added according to the live weight and extracted with water (25~100℃), or ethanol is added to the live weight and heated to room temperature (25℃) or warmed (50℃). Extracting at ~70°C for 5-72 hours; and (c) long-term storage of the extract directly or after evaporation with ethanol.

The cosmetic composition of the present invention as described above may contain ingredients commonly used in cosmetic compositions, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers. Includes.

Such cosmetic compositions can be prepared in any formulation commonly prepared in the art. For example, it may be any one formulation selected from the group consisting of high viscosity emulsion formulation, low viscosity emulsion formulation, and solubilization formulation, but is not limited thereto.

Depending on the formulation to be manufactured, it may contain cosmetic composition ingredients commonly used in the technical field to which the invention pertains. For example, it is formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, and spray. It may be possible, but it is not limited to this. More specifically, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, sun lotion, sunscreen, makeup base, BB cream, cleansing cream, cleansing foam, cleansing water, pack, stick product, balm ( It can be manufactured as a balm type product, spray or powder formulation.

Depending on the formulation to be manufactured, additional water-phase components, oil-phase components, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, preservatives, fragrances, etc. can be selected and added.

Hereinafter, the present invention will be described in detail through examples and experimental examples. It is clear that the present invention is not limited by the description of the above-described embodiments.

[Example]

The following rock pine extract was prepared and used in the test described later.

Example 1: Chitosan oligosaccharide foliar application, before flower stalk extraction, water extraction by cold steeping method (30°C, 30 hours)

Example 2: Chitosan oligosaccharide foliar application, before flower stalk emergence, hot water extraction (100°C, 10 hours)

Example 3: Chitosan oligosaccharide foliar application, before flower stalk emergence, 70% by weight ethanol room temperature extraction (30°C, 20 hours)

Example 4: Chitosan oligosaccharide foliar application, before flower stalk emergence, extraction with 70% by weight ethanol heating (60°C, 10 hours)

Example 5: Chitosan oligosaccharide foliar application, flower stalk extraction, and water extraction by cold immersion method (30°C, 30 hours)

Example 6: Chitosan oligosaccharide foliar application, flower stalk extraction, hot water extraction (100°C, 10 hours)

Example 7: Chitosan oligosaccharide foliar application, flower stalk extraction, 70% by weight ethanol room temperature extraction (30°C, 20 hours)

Example 8: Chitosan oligosaccharide foliar application, flower stalk extraction, 70% by weight ethanol heating extraction (60°C, 10 hours)

In addition, Comparative Examples 1 to 8 were the same as each of the Examples, except that foliar application of chitosan oligosaccharide was not applied.

[Test example]

1. Elastase inhibition rate measurement

The effect of inhibiting the activity of Elastase, an enzyme that decomposes elastin, was confirmed as follows.

Elastase derived from human white blood cells was used, and the synthetic substrate MeOSuc-Ala-Ala-Pro-Val-pNA was used as the elastase substrate. A 100mM Tris (pH 7.5) solution was used as the buffer solution. Elastase was used in a final amount of 0.2mU using a buffer solution. In addition, the synthetic substrate of elastase was made into a 100mM solution using DMSO and then diluted using a buffer solution to reach a final concentration of 0.5mM.

At this time, quercetin, known as an elastase inhibitor, was used as a positive control. The reaction was carried out in a 96-well plate and reacted at room temperature for 20 minutes. Absorbance was measured at 405 nm at 1-minute intervals using a spectrophotometer, and the slope of absorbance versus time was determined to be the enzyme activity. The elastase inhibition rate was calculated as follows and the results are shown in Table 1.

Elastase inhibition rate Positive control group 55.34% Example 1 62.78% Example 2 68.32% Example 3 68.85% Example 4 77.41% Example 5 58.78% Example 6 63.15% Example 7 64.31% Example 8 67.25% Comparative Example 1 43.75% Comparative Example 2 44.23% Comparative Example 3 44.25% Comparative Example 4 47.12% Comparative Example 5 40.28% Comparative Example 6 41.58% Comparative Example 7 43.15% Comparative Example 8 45.52%

Looking at Table 1, the examples according to the present invention all showed improved inhibition rates compared to the positive control group, and also showed improved results before and after flower stalk sprouting, superior inhibition rates when used in ethanol rather than water, and when heated rather than at room temperature.

Compared to the examples of the present invention, the comparative examples without foliar application of chitosan oligosaccharide showed lower results than the positive control group and showed significantly different results from the examples of the present invention.

2. Collagenase inhibition rate measurement

The effect of inhibiting the activity of collagenase, an enzyme that decomposes collagen, was confirmed as follows.

Collagenase derived from rat pancreas was used, and the synthetic substrate N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala was used as the collagenase substrate. The buffer solution was a solution containing 50mM Tricine, 10mM CaCl ₂ ·H ₂ O, and 400mM NaCl (pH 7.5). The synthetic substrate was dissolved to a concentration of 10mM using a buffer solution and then diluted to a final concentration of 75 μM. Collagenase was dissolved at a concentration of 1 unit/ml using cold 4-degree distilled water and then diluted with a buffer solution to a final concentration of 0.05 unit/ml.

Epigallocatechin gallate (EGCG), which is known to have a collagenase inhibitory effect, was used as a positive control, and the reaction was carried out in a 96-well plate and reacted at room temperature for 10 minutes. Absorbance was measured at 345 nm at 1-minute intervals using a spectrophotometer, and the maximum slope of absorbance versus time was determined to be the enzyme activity. The collagenase inhibition rate was calculated as follows and the results are shown in Table 2.

Collagenase inhibition rate Positive control group 65.15% Example 1 72.45% Example 2 78.31% Example 3 78.23% Example 4 87.42% Example 5 68.18% Example 6 73.29% Example 7 74.32% Example 8 77.56% Comparative Example 1 53.15% Comparative Example 2 54.25% Comparative Example 3 55.72% Comparative Example 4 57.52% Comparative Example 5 50.17% Comparative Example 6 51.42% Comparative Example 7 53.83% Comparative Example 8 55.29%

Looking at Table 2, the examples according to the present invention all showed improved inhibition rates compared to the positive control group, and also showed improved results before and after flower stalk sprouting, superior inhibition rates when used in ethanol rather than water, and when heated rather than at room temperature.

Compared to the examples of the present invention, the comparative examples without foliar application of chitosan oligosaccharide showed lower results than the positive control group and showed significantly different results from the examples of the present invention.

3. Measurement of cell proliferation effect

To confirm the activation of skin cell proliferation, the cell proliferation activity was measured using human fibroblasts.

After adding the samples of Examples and Comparative Examples to the culture medium, human fibroblasts were cultured, MTT reduction was performed, the formazan product was extracted with isopropyl alcohol, and the absorbance was measured at 570 nm to determine cell proliferation activity. were compared. At this time, culture medium without samples was used as a negative control. The measured results were calculated numerically using the following formula.

Cell proliferation ability (%) = absorbance in each sample group/absorbance in control group

Cell proliferation activity control group 100% Example 1 135.32% Example 2 141.25% Example 3 142.43% Example 4 155.81% Example 5 129.12% Example 6 135.62% Example 7 135.41% Example 8 145.82% Comparative Example 1 112.82% Comparative Example 2 113.65% Comparative Example 3 115.20% Comparative Example 4 118.32% Comparative Example 5 108.54% Comparative Example 6 109.35% Comparative Example 7 110.43% Comparative Example 8 112.38%

Looking at Table 3, both the examples and comparative examples of the present invention showed more than 100% of the cell proliferation activity (100%) of the control group, but the examples of the present invention showed significantly higher results than the comparative examples.

In addition, in the examples according to the present invention, improved results were shown before the flower stalks were picked compared to after they were picked, and excellent activity was shown when the flower stalks were heated in ethanol rather than water and at a temperature higher than room temperature.

4. Evaluation of water retention capacity when applied to the human body

In order to evaluate the skin moisturizing effect of the compositions of Examples and Comparative Examples, the water retention capacity was measured on the skin to which the materials of Examples and Comparative Examples were applied as follows.

To measure water retention capacity, a certain amount (0.1g/4㎠) was applied to the inside of the forearm of 20 test subjects in a constant temperature and humidity room with an room temperature of 22℃ and a relative humidity of 45%, and the skin was measured before application, 1 hour after application, and 2 hours after application. The moisture content was measured. The measuring device used was a moisture content meter (corneometer CM820, Courage + Khazaka, Cologne, Germany), which measures the moisturizing power by measuring the electrical capacitance of the skin according to the moisture content of the skin, and the results are shown in Table 4 below. .

electrical conductivity Before application 1 hour after application 2 hours after application Example 1 50 83 77 Example 2 50 84 78 Example 3 50 84 78 Example 4 50 88 83 Example 5 50 74 71 Example 6 50 78 72 Example 7 50 77 73 Example 8 50 81 76 Comparative Example 1 50 54 50 Comparative Example 2 50 56 52 Comparative Example 3 50 56 53 Comparative Example 4 50 58 52 Comparative Example 5 50 53 49 Comparative Example 6 50 54 51 Comparative Example 7 50 55 50 Comparative Example 8 50 56 50

From Table 4 above, it was confirmed that the composition according to the present invention had a significantly superior moisture retention ability compared to the composition of the comparative example, which proved that it was a material with an excellent moisturizing effect.

In addition, in the examples according to the present invention, improved results were shown before the flower stalks were picked compared to after they were picked, and excellent activity was shown when the flower stalks were heated in ethanol rather than water and at a temperature higher than room temperature.

5. Skin whitening: Check the effect of inhibiting melanin production

The compositions of Examples and Comparative Examples were added to the culture medium of B-16 mouse melanoma cells, and the whitening effect at the cellular level was tested. (Lotan R, Lotan D Cancer Res 40:3345-3350, 1980)

Before the experiment, toxicity was evaluated for rat melanoma cells, a non-toxic concentration was selected, and whitening evaluation was performed.

The final concentration was tested to be 100㎍/ml, and arbutin as a control was added to the medium to be 100㎍/ml, treated with B-16 melanoma cells, and cultured for 3 days.

Afterwards, the cells were treated with trypsin, removed from the culture vessel, centrifuged, and melanin was extracted. 1 ml of sodium hydroxide solution (1N concentration) was added to the removed cells and boiled for 10 minutes to dissolve the melanin. The amount of melanin produced was measured by measuring the absorbance at 400 nanometers (nm) using a spectrophotometer.

The amount of melanin was measured by expressing the absorbance per unit cell (10 ⁶ cells), and the amount of melanin production relative to the control group was calculated as an inhibition rate (%), and the results are summarized in Table 4. Additionally, the experiment was repeated three times.

sample Melanin production amount Inhibition rate (%) negative control 0.065±0.002 - Positive control group 0.033±0.001 49% Example 1 0.015±0.003 77% Example 2 0.015±0.001 77% Example 3 0.015±0.002 77% Example 4 0.010±0.003 85% Example 5 0.021±0.001 67% Example 6 0.016±0.003 75% Example 7 0.016±0.001 75% Example 8 0.012±0.002 81% Comparative Example 1 0.036±0.003 44% Comparative Example 2 0.033±0.003 49% Comparative Example 3 0.033±0.001 49% Comparative Example 4 0.027±0.001 58% Comparative Example 5 0.036±0.003 44% Comparative Example 6 0.036±0.003 44% Comparative Example 7 0.036±0.003 44% Comparative Example 8 0.036±0.003 44%

As can be seen from the results in Table 5, the composition of the present invention was found to have a significantly superior melanin production inhibition ability against cultured rat melanoma cells compared to arbutin, a previously known whitening substance, and compared with this. In the case of the example, the ability to inhibit melanin production was significantly inhibited compared to that of the present invention.

In addition, in the examples according to the present invention, improved results were shown before the flower stalks were picked compared to after they were picked, and excellent activity was shown when the flower stalks were heated in ethanol rather than water and at a temperature higher than room temperature.

Prescription example: Transparent moisture gel

Examples of formulations of transparent water gel among cosmetics containing the Bacon pine extract of the present invention are as follows (see Table 6).

One Purified water balance 3,727 2 carbomer 0.5 25 3 Aloe vera gel water 3 150 4 glycerin 5 250 5 witch hazel water 3 150 6 Hyaluronic acid 1% 5 250 7 allantoin 0.3 15 8 Rock pine extract 5 250 9 Coenzyme Q10 0.2 10 10 Adenosine 0.2 10 11 Triethanolamine 0.5 20 12 PEG-60 Hydrogenated Castor Oil 0.5 25 13 ethanol 0.7 35 14 menthol 0.1 5 15 Bassam 1.5 75 100.00 4,997

The rock pine extract used here is the extract obtained in Example 4.

Sensory evaluation of transparent water gel (White Lisulbinghwa)

The sensory evaluation questionnaire for the transparent water gel created in this project was prepared as shown in Table 7 below, and the results were derived from the table below.

Transparent moisture gel (White Lisulbinghwa) cosmetics test questionnaire
Jeonbuk Hub Industry-Academy-Research Cooperation Foundation

Are you a man who writes new products? Are you a woman?
What is your age when using a new product?
Where are you working on new products?

1. How is the design of the new product?
① Very good ② Excellent ③ Average ④ Below average ⑤ Below expectations
2. Is the new product’s container convenient to use?
① Very good ② Excellent ③ Average ④ Below average ⑤ Below expectations
3. What do you think of the design colors of the new product?
① Very good ② Excellent ③ Average ④ Below average ⑤ Below expectations
4. How is the application quality of the new product?
① Very good ② Excellent ③ Average ④ Below average ⑤ Below expectations
5. What does the new product smell like?
① Very good ② Excellent ③ Average ④ Below average ⑤ Below expectations
6. What is the formulation of the new product?
① Very good ② Excellent ③ Average ④ Below average ⑤ Below expectations
7. How does the new product feel?
① Very good ② Excellent ③ Average ④ Below average ⑤ Below expectations
8. Is there any differentiation between the new product and those of other companies?
① Very good ② Excellent ③ Average ④ Below average ⑤ Below expectations

*Very good (5 points), Excellent (4 points), Average (3 points), Below average (2 points), Below expectations (1 point)

The sensory evaluation results are as follows. It was evaluated as excellent overall.

How is Baekrisul Moisture Gel (tentative name: Baekrisulbinghwa) applied? Answer Options Response Percent Response Count very excellent 5.6% One Great 72.2% 13 commonly 11.1% 2 below average 5.6% One below expectations 5.6% One answered question 18

What is the formulation of the new product? Answer Options Response Percentage Response Count very excellent 0% 0 Great 62.5% 10 commonly 37.5% 6 below average 0% 0 below expectations 0% 0 answered question 16

How does the new product feel? Answer Options Response Percentage Response Count very excellent 0% 0 Great 93.3% 14 commonly 6.7% One below average 0% 0 below expectations 0% 0 answered question 15

Is there any differentiation between the new product and those of other companies? Answer Options Response Percentage Response Count very excellent 0% 0 Great 46.7% 7 commonly 53.3% 8 below average 0% 0 below expectations 0% 0 answered question 15

Overall, the sensory evaluation conducted by experts gave a score of 3.66 (out of 5). The transparent water gel produced in this example was proven to be excellent.

As seen above, the cosmetic composition according to the present invention contains as an active ingredient an extract with improved effectiveness compared to the conventional rock pine extract, and promotes skin whitening, moisturizing, elasticity, and regeneration of the skin's dead skin cells, thereby maintaining moisture and pulling, It has the effect of relieving dryness, whitening the skin, and increasing elasticity, so it can be very suitable and useful as an anti-aging cosmetic composition.

It is obvious to those skilled in the art that the present invention is not limited to the above embodiments and can be implemented with various modifications or variations without departing from the technical gist of the present invention. will be.

Claims (6)

Planting rock pine seedlings, diluting chitosan oligosaccharides in water at a weight ratio of 1:100 to 1:1,000, cultivating and harvesting them by foliar application at intervals of several days, and containing rock pine extract extracted from the harvested rock pine as an active ingredient,
A cosmetic composition, characterized in that the harvested rock brush is harvested before flower stalks emerge. delete According to paragraph 1,
A cosmetic composition wherein the extraction solvent for the rock pine extract is water or ethanol. According to paragraph 3,
A cosmetic composition characterized in that the extraction solvent of the Bawisol extract is ethanol heated to 50-70°C. In the method of manufacturing a cosmetic composition,
Planting rock brush seedlings;
Diluting chitosan oligosaccharide in water at a weight ratio of 1:100 to 1:1,000 to the rock brush seedlings and then fertilizing the leaves at intervals of several days;
Harvesting the cultivated rock brush;
Obtaining rock brush extract using harvested rock brush;
Preparing a cosmetic composition containing the rock pine extract;
Including,
A method of producing a cosmetic composition, characterized in that in the step of harvesting the rock brush, the flower stalk of the rock brush is harvested before emergence. delete