KR102549086B1 - Composition for skin anti-polution comprising extract of cirsium setidens as an effective ingredient - Google Patents

Abstract

The present invention relates to a skin anti-pollution composition comprising gondre extract as an active ingredient.
The skin anti-pollution composition according to the present invention contains gondre extract as an active ingredient, thereby having an anti-fouling effect of protecting the skin from chemicals toxic to skin cells. Specifically, when the composition is treated on skin treated with toxic chemicals, death of skin cells can be minimized and skin conditions deteriorated due to toxic chemicals can be improved.

Description

Composition for skin anti-pollution containing gondre extract as an active ingredient

The present invention relates to a skin anti-pollution composition comprising gondre extract as an active ingredient.

The skin is a body part directly exposed to the external environment, and not only serves as a protective film to protect important organs of our body from the outside, but also controls moisture evaporation and protects the body from external infections. However, modern people's skin is constantly exposed to environmental pollutants and strong ultraviolet rays generated during the manufacturing or disposal of various chemical substances developed with industrialization, complaining of skin troubles such as erythema, swelling, itching, and rapid skin aging. Cases are increasing.

The environmental contaminants not only penetrate into the body but also adversely affect the skin when they come into direct contact with the skin, such as dermatitis, skin rash, and skin necrosis. Accordingly, there is an increasing demand for cosmetics capable of protecting the skin from environmental pollutants as well as ultraviolet rays.

Benzopyrene is exemplified as belonging to the above environmental pollutants. The benzopyrene is produced due to incomplete combustion such as automobile exhaust gas or cigarette smoke, and is a toxic substance that causes cell damage through metabolic conversion to epoxide and reaction with DNA. Recently, as the news that it causes atopic dermatitis has been heard, adverse effects on the skin have been revealed, and it is one of the dangerous substances that can cause skin deterioration due to problems such as cell mutation and cell damage.

In addition, nitrogen oxides belong to the environmental pollutants. The nitrogen oxide is a compound composed of nitrogen and oxygen, and includes nitrogen monoxide, nitrogen dioxide, dinitrogen monoxide, dinitrogen trioxide, dinitrogen tetroxide, dinitrogen pentoxide, and the like, and ionized products thereof. In general, nitrogen gas is stable and it is difficult for the atoms constituting it to be split into two, but the bond can be broken when a very high temperature is applied. Often, high temperatures are formed inside the engine of a vehicle, so that the exhaust gas of the vehicle contains a large amount of nitrogen oxides. The nitrogen oxide may include ammonium or a salt thereof. Nitric acid, another form of nitrogen oxide, reacts with water to form nitric acid and is also responsible for acid rain. The nitric acid combines with ammonium, an alkaline substance in the air, to produce ammonium nitrate. The ammonium nitrate is a particulate matter in the air, which is secondary fine dust and adversely affects the human body. When the ammonium nitrate comes into contact with the human body, it causes inflammation in the eyes, nasal mucosa and skin.

In addition, formaldehyde belongs to the environmental pollutants. The formaldehyde is a colorless gas with a strong irritating odor at room temperature and is also called methaneal. The formaldehyde is easily produced when materials containing carbon are incompletely burned, and is synthesized when sunlight and oxygen are applied to hydrocarbons such as methane in the air. The formaldehyde is a skin allergen, and when it comes into contact with the skin, it can cause problems such as skin rash, skin irritation, and dermatitis.

The above-described contaminants cause direct inflammation or necrosis when in contact with the skin at high concentrations, and even residual amounts in the air cause continuous stress on the skin when continuously contacted, thus acting as a cause of skin aging.

Compared to the effects of the above pollutants on the skin, the public's awareness of the risks is still low, but as issues such as fine dust have recently emerged, the risks are gradually attracting attention, and people who are gradually aware of the dangers of pollutants The number is growing.

Recently, cosmetics to prevent the penetration or attachment of contaminants or pollutants to the skin, or detergents with enhanced cleaning power of contaminants have been developed, but these products have insignificant ability to prevent penetration of contaminants into the skin, and only pollutants from the skin However, there is a limitation that it does not fundamentally protect the skin from pollutants, and since it contains chemicals as main components, many cases of causing skin trouble have been reported.

The present inventors were studying to develop a material that can fundamentally improve the condition of skin contaminated by chemicals among natural products that are not irritating to the skin, and found that gondre extract has anti-contamination effect on the skin and completed the present invention. I did.

KR 10-2019-0078911 A KR 10-1928195 B

An object to be solved by the present invention is to provide a composition for skin anti-pollution, which has a skin anti-contamination effect against pollutants by containing gondre extract as an active ingredient.

In order to achieve the above object, the present invention contains a gondre extract as an active ingredient, and has a skin antifouling effect against one or more contaminants selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes and nitrogen oxides. Provided is a composition for pollution.

According to one embodiment of the present invention, the gondre extract may be extracted with water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

According to one embodiment of the present invention, the gondre extract may be irradiated with ultraviolet UVB at 350 to 1000 mJ/cm ² before or after the gondre is extracted.

According to an embodiment of the present invention, the polycyclic aromatic hydrocarbon may include benzopyrene, the nitrogen oxide may include ammonium nitrate, and the aldehydes may include formaldehyde.

According to one embodiment of the present invention, the composition can prevent or inhibit skin contamination, skin cell death, or inhibition of skin cell proliferation by the contaminants.

According to one embodiment of the present invention, the composition can alleviate skin irritation or skin inflammation caused by the contaminants.

According to one embodiment of the present invention, the composition may be a composition for protecting skin cells against the pollutants or a composition for inhibiting skin cell death.

According to one embodiment of the present invention, the composition can reduce the activity of XRE (Xenobiotic Response Element) overexpressed by the contaminant.

According to one embodiment of the present invention, the composition can reduce the amount of NO increased by the contaminant.

According to one embodiment of the present invention, the skin anti-pollution composition may be a cosmetic composition or a health functional food composition.

The skin anti-pollution composition according to the present invention contains gondre extract as an active ingredient, thereby having an anti-fouling effect of protecting the skin from chemicals toxic to skin cells. Specifically, when the composition is treated on skin treated with toxic chemicals, death of skin cells can be minimized and skin conditions deteriorated due to toxic chemicals can be improved.

Hereinafter, the present invention will be described in detail.

In the present specification, "skin" refers to a tissue covering the body surface of an animal, and is a broad concept that includes not only the tissue covering the body surface such as the face or body, but also the scalp.

In this specification, "extract" includes all substances obtained by extracting components of natural substances, regardless of extraction method, extraction solvent, extracted component or extract form, and also includes substances obtained by extracting components of natural substances. It is a broad concept that includes all substances that can be obtained by processing or processing by other methods after extraction.

In the present specification, “anti-pollution” or “anti-pollution” may mean preventing or improving skin condition deterioration due to substances toxic to the skin.

In the present specification, "decrease in skin condition" may refer to a state in which skin troubles such as skin irritation and skin inflammation appear due to contaminants, necrosis or death of skin cells, or inhibition of skin cell proliferation.

In addition, in the present specification, “skin cell protection” may mean minimizing necrosis or death of skin cells or improving inhibition of cell proliferation.

In the present specification, “skin irritation” may be at least one selected from the group consisting of irritation such as itching, stinging, and burning, erythema, and edema.

In the present specification, "skin trouble" is a term that includes all of the above skin irritations and the like.

According to one aspect of the present invention, the present invention contains a gondre extract as an active ingredient, and has an antifouling effect on the skin against at least one pollutant selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes, and nitrogen oxides. A composition for anti-pollution is provided.

Gondre refers to the 'Goryeo Thistle', a perennial plant belonging to the Asteraceae family. In particular, in Gangwon-do, young shoots are called 'gondere' and are mainly eaten as vegetables. It contains polyphenols, dietary fiber, minerals and vitamins, and is known to show various physiological activities including anticancer activity.

The gondre extract may be obtained by extracting gondre leaves, stems, or aerial parts of the gondre plant. After harvesting, the gondre may be washed and used as is or dried. As the drying method, drying in the sun, drying in the shade, hot air drying, cold air drying, and natural drying can all be used, and to increase the extraction efficiency, gondre or its dry body can be pulverized with a grinder and used.

The extraction solvent for extracting the gondre extract may be water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. Examples of the lower alcohol include 20 to 80% methanol, ethanol, butanol or propanol.

The extraction solvent is not particularly limited, but the extract extracted with 60 to 80% aqueous ethanol solution preferably acts on the skin antifouling effect.

The gondre is preferably mixed with an extraction solvent at a weight ratio of 1:2 to 20, preferably 1:5 to 15, and more preferably 1:8 to 12 in terms of extraction yield of active ingredients.

The extraction may be performed by a heating extraction method, a cold needle extraction method, a reflux cooling extraction method, an ultrasonic extraction method, an ultra-high pressure extraction method, or the like, and there is no limitation as long as the extraction method is obvious to those skilled in the art. The extraction may be performed at room temperature, but for more efficient extraction, it may be performed under heated conditions, and extraction may be performed at a temperature of 20 to 100 ° C, preferably about 40 to 70 ° C, more preferably 50 to 60 ° C. It may, but is not limited thereto. The extraction time may be performed for 1 to 10 hours, preferably 2 to 8 hours, and more preferably 4 to 8 hours, but is not limited thereto, and may vary depending on conditions such as extraction solvent and extraction temperature. The extraction may be repeated once or more, preferably 1 to 5 times, more preferably 2 to 3 times, in order to obtain a larger amount of the active ingredient.

On the other hand, in order to enhance the skin antifouling activity of the gondre extract, ultraviolet UVB may be irradiated at 350 to 1000 mJ/cm ² before or after the gondre is extracted, and irradiation with ultraviolet UVB before the gondre is extracted is antifouling More preferred in terms of increased activity.

In this specification, "ultraviolet (UV)" is a ray of light in a wavelength region shorter than the violet of visible light. There are three types of UV: UVA (200 ~ 280nm), UVB (280 ~ 320nm), and UVC (320 ~ 400nm). UVC is almost absorbed by the ozone layer, and only UVA and UVB reach the earth's surface. The term "ultraviolet ray UVB" of the present invention is an ultraviolet ray having a wavelength of 280 to 320 nm.

The irradiation amount of the ultraviolet UVB may be 350 to 1000 mJ/cm ² , preferably 600 to 950 mJ/cm ² , and more preferably 800 to 900 mJ/cm ² .

When the irradiation amount of the ultraviolet UVB is less than the lower limit, the effect of ultraviolet irradiation becomes insignificant, and when it exceeds the upper limit, the skin antifouling activity of gondre may rather decrease.

In addition, the gondre extract may be a fraction obtained by re-fractionating an extract obtained by extracting gondre with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof with a second organic solvent. Preferably, the gondre extract may be a fraction obtained by re-fractionating the ethanol extract of gondre with hexane, ethyl acetate, butanol or water.

The term "extract" used herein while referring to gondre includes not only a crude extract obtained by treating an extraction solvent, but also a processed product of gondre extract. For example, the gondre extract can be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

Further, the gondre extract of the present invention has the meaning of including gondre processed products, such as gondre powder, formulated so that gondre can be administered to animals in a broad sense. Although the experiment was conducted with gondre in the present invention, it would be expected by those skilled in the art that the desired effect can be achieved even in the form of a gondre processed product.

The skin anti-pollution composition comprising the gondre extract of the present invention as an active ingredient has a skin anti-pollution effect against the pollutants. Specifically, the composition can alleviate or improve skin contamination, skin cell death or skin cell proliferation inhibition, or skin condition deterioration caused by the contaminant. In addition, the composition can alleviate or improve skin irritation or skin inflammation caused by contaminants.

In addition, the skin anti-pollution composition comprising the gondre extract of the present invention as an active ingredient may be a composition for protecting skin cells against the pollutants or a composition for inhibiting skin cell death.

The contaminants may include at least one selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes, and nitrogen oxides.

Specifically, the polycyclic aromatic hydrocarbon may include benzopyrene, and the aldehydes may include formaldehyde. In addition, the nitrogen oxides may include nitrogen oxides represented by the chemical formula NOx, for example, NO, NO ₂ , N ₂ O, N ₄ O, N ₂ O ₃ , N ₂ O ₄ , N ₂ O ₅ , N ₄ O ₆ or ionized products thereof. In addition, the nitrogen oxides may include ammonium or a salt thereof, and for example, the salt of the ammonium may include ammonium nitrate, which is a nitrate of ammonium.

The benzopyrene is produced due to incomplete combustion such as automobile exhaust gas or cigarette smoke, and is a toxic substance that causes cell damage through metabolic conversion to epoxide and reaction with DNA. Recently, as news that benzopyrene induces atopic dermatitis has been encountered, adverse effects on the skin have been revealed, and it is one of the dangerous substances that can cause skin deterioration due to problems such as cell mutation and cell damage.

The formaldehyde is a colorless gas with a strong irritating odor at room temperature and is also called methaneal. The formaldehyde is easily produced when materials containing carbon are incompletely burned, and is synthesized when sunlight and oxygen are applied to hydrocarbons such as methane in the air. The formaldehyde is a skin allergen, and may cause problems such as skin rash, skin necrosis, and dermatitis upon contact with the skin.

The nitrogen oxide is a compound composed of nitrogen and oxygen, and includes nitrogen monoxide, nitrogen dioxide, dinitrogen monoxide, dinitrogen trioxide, dinitrogen tetroxide, dinitrogen pentoxide, and the like, and ionized products thereof. In general, nitrogen gas is stable and it is difficult for the atoms constituting it to be split into two, but the bond can be broken when a very high temperature is applied. Often, high temperatures are formed inside the engine of a vehicle, so that the exhaust gas of the vehicle contains a large amount of nitrogen oxides. The nitrogen oxide may include ammonium or a salt thereof. Nitric acid, another form of nitrogen oxide, reacts with water to form nitric acid and is also responsible for acid rain. The nitric acid combines with ammonium, an alkaline substance in the air, to produce ammonium nitrate. The ammonium nitrate is a particulate matter in the air, which is secondary fine dust and adversely affects the human body. When the ammonium nitrate comes into contact with the human body, it causes inflammation in the eyes, nasal mucosa and skin.

In the present invention, the skin anti-pollution composition containing the gondre extract as an active ingredient suppresses the expression of Xenobiotic Response Element (XRE), which is overexpressed due to the contaminant, thereby reducing skin irritation such as skin irritation caused by the contaminant. It can alleviate trouble. That is, the composition of the present invention may seek fundamental skin defense against contaminants.

The skin has a metabolic mechanism to defend against external xenobiotics such as contaminants. Specifically, through a receptor called AHR (Aryl Hydrocarbon Receptor) of skin keratinocytes, a xenobiotic response element (XRE) is activated to regulate defense factors to protect the skin from these xenobiotics. However, in a situation where the level of air pollution is serious as in these days, XRE may be excessively activated in the process of defending the skin against pollutants, resulting in skin troubles such as skin irritation.

In the present invention, the "anti-fouling effect of the skin against contaminants" is to prevent or improve the deterioration of the skin condition, such as the above-described contaminants penetrating into the skin and causing damage, specifically, the skin caused by the contaminants. It may mean an effect of alleviating skin troubles such as irritation or skin inflammation. In addition, the alleviation of skin irritation or skin inflammation caused by contaminants of the present invention may mean protection/reduction of skin irritation caused by contaminants, alleviation of skin inflammation, maintenance of skin homeostasis, etc., but is not limited thereto.

In an atmospheric environment with many pollutants, XRE is excessively activated in the process of protecting the skin, causing skin irritation and moisture loss. The composition of the present invention can alleviate skin troubles such as skin irritation and moisture loss caused by pollutants by suppressing the overactive XRE.

Specifically, the expression of XRE by AHR promotes the synthesis of biodefense substances such as cytochrome family members (CYP1A1, CYP1B1) in skin cells. However, if this reaction continues, irritation such as itching, tingling, and burning, and skin irritation such as erythema or swelling may be induced, and as a result, symptoms such as psoriasis, atopy, and allergies may appear or worsen. Continuous exposure of the skin to harmful environments causes chronic skin diseases, and in particular, skin irritation caused by pollutants such as polycyclic aromatic hydrocarbons, aldehydes, and nitrogen oxides is more of a problem of deteriorating skin texture. Furthermore, it causes the development of a disease or disease. Since there is a limit to coping with these individual skin diseases, it is most important to control the activity of XRE overexpressed due to contaminants.

In a specific embodiment, XRE was artificially overexpressed by treating keratinocytes with harmful chemicals such as benzopyrene, and then, as a result of treatment with the gondre extract of the present invention, it was confirmed that the overactivated XRE was significantly reduced did Through this, it can be seen that the gondre extract can improve the deterioration of skin conditions caused by pollutants by controlling the overactivity of XRE caused by pollutants such as benzopyrene.

The skin anti-pollution composition of the present invention as described above may be used as a cosmetic composition or a health functional food composition.

The "cosmetic composition" of the present invention contains the gondre extract as an active ingredient, thereby having a skin antifouling effect, specifically, an improvement effect on any one or more symptoms of skin contamination by the contaminants, skin cell death, or inhibition of skin cell proliferation. .

In a preferred embodiment of the present invention, the gondre extract may be present in the composition in any amount desired to provide a skin antifouling effect. According to one aspect, the gondre extract is 10 to 200 μg/ml, preferably 20 to 100 μg/ml, more preferably 25 to 50 μg/ml, most preferably 50 μg/ml, based on the total amount of the cosmetic composition. It can be included in a concentration of ml. At this time, if the content of the gondre extract is less than the lower limit, the skin antifouling effect may not be exhibited to a desired degree. Conversely, when the concentration exceeds the upper limit, the skin antifouling effect does not increase as much as the concentration increases, or there may be toxicity. That is, when the gondre extract of the present invention is included in the composition in the above range, an excellent antagonistic effect against skin contamination can be realized, formulation and product stability can be achieved, and excellent effects can be exhibited at an optimal content in terms of economy.

Ingredients included in the cosmetic composition include ingredients commonly used in cosmetic compositions in addition to the gondre extract as an active ingredient, for example, antioxidants, stabilizers, solubilizers, vitamins, and conventional adjuvants and carriers such as flavoring agents. can include more.

The cosmetic composition is a suspension, emulsion, paste, gel, cream, atopic soap, cleansing foam, cleansing cream, cleansing water, bath agent, skin softener, skin toner, lotion, cream, essence, astringent, emulsion, gel, lipstick, It may be any one formulation selected from the group consisting of a spray, shampoo, rinse, treatment, body cleanser, pack, massage, face powder, compact, foundation, two-way cake, and makeup base.

When the formulation of the cosmetic composition is a paste, cream or gel, vegetable oil, wax, starch, cellulose, silicone, bentonite, silica, zinc oxide, etc. may be further included as a carrier component, and when the formulation is a powder, lactose as a carrier component , silica, aluminum hydroxide, calcium silicate, etc. may further contain powder. When the formulation of the cosmetic composition is an emulsion, it may further include a solvent, a solubilizing agent or an emulsifying agent as a carrier component, such as water, ethanol, glycerin, ethyl carbonate, ethyl acetate, propylene glycol, butylene glycol , 1,2-hexanediol, glycerol aliphatic esters, and sorbitan fatty acid esters.

When the formulation of the cosmetic composition is a suspension, as a carrier component, a liquid diluent such as water, ethanol, or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester , microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, and the like may be further included.

The cosmetic composition of the present invention may be used alone or overlappingly applied, or may be used overlappingly with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the user's skin condition or taste.

The "functional health food composition" of the present invention refers to food (including processing) manufactured (including processing) according to legal standards using raw materials or ingredients having functional properties useful for the human body (Article 3, Subparagraph 1 of the Health Functional Food Act). . The term 'health functional food' may differ in terminology or scope from country to country, but 'Dietary Supplement' in the US, 'Food Supplement' in Europe, 'Health Functional Food' or 'Health Functional Food' in Japan. It may correspond to 'Food for Special Health Use (FoSHU)' or 'Health Food' in China.

The health functional food composition may additionally contain food additives, and the suitability as a food additive is subject to the standards and standards for the item in accordance with the general rules and general test methods of the 'Food Additive Code' unless otherwise specified.

When preparing a health functional food composition using the gondre extract as an active ingredient, the gondre extract does not need to be particularly limited as long as the content exhibits skin antifouling activity. For example, 0.01 to 99% by weight, 0.5 to 95% by weight, 1 90% by weight, 2 to 80% by weight, 3 to 70% by weight, 4 to 60% by weight, may be included in 5 to 50% by weight.

The daily dose of the gondre extract, which is an active ingredient in the health functional food composition, varies depending on the condition, weight, presence or absence, degree and duration of the disease of the subject to be administered, but can be appropriately selected by a person skilled in the art. However, for a desirable effect, the gondre extract in the health functional food composition is 10 to 200 μg/ml, preferably 20 to 100 μg/ml, more preferably 25 to 50 μg/ml, based on solid content per day. It is preferably administered at a concentration of 50 μg/ml. At this time, if the content of the gondre extract is less than the lower limit, the skin antifouling effect may not be exhibited to a desired degree. Conversely, when the concentration exceeds the upper limit, the skin antifouling effect does not increase as much as the concentration increases, or there may be toxicity. On the other hand, as a result of the in vitro experiment, when the concentration of the gondre extract of the present invention was within the above range, a significant effect on skin anti-fouling was shown, but side effects such as cytotoxicity were not shown. The frequency of administration does not need to be particularly limited, but can be adjusted by a person skilled in the art within the range of three times a day to once a week. In the case of long-term intake for the purpose of health and hygiene or health control, it may be less than the above range.

In addition, the health functional food composition may be prepared by mixing the gondre extract with other active ingredients known to have an antagonistic effect on skin contamination.

According to a preferred embodiment of the present invention, the health functional food composition of the present invention has the advantage of having a more excellent skin anti-fouling effect by ingesting it in the form of inner beauty food. The inner beauty refers to food that changes the skin constitution to be healthy by absorbing various ingredients that are good for the skin into the body. You can select and consume the inner beauty food that suits your individual skin condition and lifestyle. More preferably, when the cosmetic composition containing the cosmetic composition and the health functional food composition are mixed in the form of inner beauty food, the skin anticontamination effect can be significantly increased compared to using only cosmetics.

Hereinafter, the present invention will be described in detail by examples, but the present invention is not limited by the following examples.

<Example>

Example 1: Preparation of gondre extract

(1) UV irradiation

Raw gondola leaves and stems were washed and dehydrated.

In addition, after putting the gondre (leaves and stems) in a container (350 * 255 * 40 mm) for UV treatment, ultraviolet rays (UVB) (300 nm) was irradiated, and the samples were turned over and mixed whenever the irradiation amount exceeded 100 mJ/m 2 . The UV irradiation was stopped when the total UVB irradiation amount reached 900 mJ/cm ² .

(2) Gondre grinding

The gondre irradiated with UVB was put into a silant cutter (USA) equipped with a cooler and pulverized to a size of 50 mesh while maintaining the temperature of the grinding space below 20 °C.

(3) Extract preparation

The pulverized gondre and 70% aqueous ethanol solution were mixed at a weight ratio of 1:10 and extracted at 50° C. for 6 hours to obtain an ethanol extract of gondre. The obtained gondre extract was filtered and concentrated under reduced pressure, and then lyophilized to powder.

Example 2: Preparation of Gondre Extract - Omitting UV Irradiation

A gondre extract was prepared in the same manner as in Example 1, except that the UV irradiation in step (1) was omitted.

< Test Example>

Experiments by concentration for each test substance were performed over three wells, and the results were expressed as the average ± standard deviation of the three measured values. To examine the statistical significance, the variation in the control value was determined by a one-way ANOVA test, and a P value of less than 5% (p < 0.05) was considered statistically significant.

Test Example 1: Cytotoxicity analysis of gondre extract

Cytotoxicity was analyzed in vitro to confirm whether the gondre extract of the present invention obtained in the above example was toxic to cells.

To this end, Raw 264.7 cells, a murine macrophage cell line, were purchased from the Korea Cell Line Bank (KCLB) and used. Penicillin-streptomycin (10,000 U/ml) was added and cultured in an incubator maintained at 37°C, 5% CO ₂ and 95% humidity.

The evaluation of cytotoxicity used the MTT assay, which showed that the dehydrogenase in the mitochondria of cells with intact metabolic processes was reduced to yellow water-soluble tetrazolium salt [3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide] (MTT ) to water-insoluble dark purple MTT formazan crystals, and is a method of evaluating cytotoxicity by measuring absorbance at appropriate wavelengths (mainly 500-600 nm). After dispensing RAW 264.7 cells into 96 wells at a concentration of 1×10 ⁵ cells/well and incubating for 24 hours, the gondre extract of the present invention was treated with 10, 50, 100, and 300 μg/mL, respectively, and cultured for 20 hours. Then, MTT at a concentration of 0.5 mg/mL was added and reacted in an incubator at 37 °C for 4 hours. Then, DMSO (dimethyl sulfoxide) was added and absorbance was measured at 540 nm using a microplate reader. The cytotoxicity was calculated by the following equation, The results are shown in Table 1 below as a relative ratio to the control group.

Cell viability (%) = (absorbance of sample treatment group / absorbance of control group) × 100

Sample (μg/mL) Cell viability (%) Control group (untreated group) 100 Example 1 10 113.1 50 108.6 100 99.7 200 92.5 Example 2 10 105.7 50 102.1 100 98.4 200 90.6

Referring to Table 1, it can be seen that the gondre extract according to the embodiment of the present invention does not show significant toxicity up to a concentration of 200 μg/mL.

Test Example 2: Skin antifouling activity (1) - skin keratinocyte protective activity

In general, to measure the degree of cell damage by pollutants using skin keratinocytes that come into direct contact with pollutants in the air, and to treat the gondre extract according to the concentration according to the embodiment to confirm the effect of inhibiting skin cell damage did

To this end, human keratinocyte HaCaT was dispensed into a 96-well plate at a concentration of 5 X 10 ³ cells/ml and cultured for 24 hours, followed by benzopyrene (10 μM), ammonium nitrate (500 μg/ml), and formaldehyde ( 1.5 μg/ml) and the gondre extract according to each concentration were treated according to the concentration and further cultured for 45 hours. Thereafter, a 5 mg/ml MTT solution was added, cultured for 3 hours, the medium was removed, 150 μl of dimethyl sulfoxide was added to each well, shaken for 10 minutes, and absorbance was measured at 570 nm. The skin cell damage inhibitory activity (cell viability) by contaminants was calculated by the following formula, and the results are shown in Table 2 below as a relative ratio to the control group.

Cell viability (%) = (absorbance of sample treatment group / absorbance of control group) × 100

Sample (μg/mL) Cell viability (% of control) Benzopyrene formaldehyde Ammonium Nitrate Control group (untreated group) 100 negative control group 58.6±3.7 75.4±4.4 64.5±6.1 Example 1 5 62.1±1.8 80.3±5.6 70.3±2.7 25 80.3±5.1 97.6±6.5 82.9±4.2 50 91.5±2.6 99.7±8.2 85.3±6.8 250 72.8±6.4 86.2±5.4 76.2±3.6 Example 2 5 61.2±1.1 79.2±3.6 69.6±3.1 25 79.6±2.8 92.8±1.9 78.6±2.7 50 82.3±3.7 94.5±5.2 80.3±3.4 250 70.5±2.3 85.3±3.8 75.7±2.9

Looking at Table 2, the gondre extract according to an embodiment of the present invention inhibits skin cell death or inhibition of skin cell proliferation caused by contaminants such as benzopyrene, formaldehyde, and ammonium nitrate, thereby exhibiting an anti-pollution effect against the contaminants. It can be confirmed that it has

Test Example 3: Skin Antifouling Activity (2) - Overactivated XRE Inhibiting Activity

Contaminants were added to human dermal keratinocytes to measure the degree of activation of xenobiotic factor (XRE) by the contaminants, and then the gondre extract according to the embodiment was treated by concentration to inhibit XRE expression overactivated by the contaminants I wanted to check the effect.

To this end, human keratinocyte HaCaT was dispensed into a 48-well plate at a concentration of 5X10 ⁴ cell/mL and cultured for 24 hours. We transiently overexpressed the XRE promoter in cells. Then, by treating the cells with benzopyrene (10 μM), it was confirmed whether the XRE promoter was overactivated by contaminants. Thereafter, the Gondre extract according to the Example was mixed with the contaminants at concentrations of 25 μg/ml and 50 μg/ml, respectively, and treated with the cells, and then cultured in a 37° C., 5% CO ₂ incubator for 24 hours. After culturing, the medium was removed, and cells were activated by adding 100 μl of passive lysis buffer to each well, and only the supernatant was taken after centrifugation at 15,000 rpm. Then, the activity of the XRE promoter was measured using the Dual-Luciferase Reporter 1000 Assay Systems kit (Promega). The effect of the gondre extract on the activity of XRE overactivated by contaminants was evaluated based on the fluorescence intensity of the wells not treated with the sample, and the results are shown in Table 3 below.

Sample (μg/mL) XRE activity (% of control) Control group (untreated group) 100 negative control group 254.3±19.7 Example 1 5 236.1±15.2 25 183.4±13.1 50 157.8±10.3 250 227.3±6.9 Example 2 5 243.8±10.7 25 208.3±12.7 50 186.2±11.5 250 231.4±16.2

Looking at Table 3, it can be seen that the gondre extract according to the embodiment of the present invention significantly reduces the XRE activity increased by contaminants such as benzopyrene. This means that the gondre extract of the present invention can effectively prevent or improve the deterioration of skin conditions caused by contaminants.

Test Example 4: Skin antifouling activity (3) - NO production inhibitory activity

Contaminants were added to human dermal keratinocytes to measure the amount of NO production increased by the contaminants, and then the Gondre extract according to the Example was treated by concentration to confirm the effect of inhibiting NO production by the contaminants.

Human keratinocytes HaCaT were dispensed in a 96-well plate at a concentration of 2×10 ⁵ cells/ml, and then cultured for 24 hours at 37° C. in a 5% CO ₂ incubator. After overnight, the medium was replaced with a fresh medium, and benzopyrene (10 μM) and the sample of Example were administered by concentration and cultured for 24 hours. After incubation, the supernatant was taken and centrifuged at 13,000 rpm for 3 minutes to collect only the supernatant, which was reacted with Griess reagent 1: 1 and absorbance was measured at 570 nm. 4.

Sample (μg/mL) NO production (% of control) Control group (untreated group) 100 negative control group 135.2±4.6 Example 1 5 131.3±9.8 25 113.3±10.2 50 109.1±5.7 250 128.6±12.4 Example 2 5 132.1±5.7 25 122.2±7.9 50 120.6±6.8 250 131.5±7.6

Referring to Table 4, it can be seen that the gondre extract according to the embodiment of the present invention significantly inhibits the production of NO increased by contaminants such as benzopyrene. This means that the gondre extract of the present invention can efficiently alleviate or improve skin troubles such as skin irritation or skin inflammation caused by contaminants.

From the above results, it was confirmed that the gondre extract of the present invention can be used for skin anti-pollution against one or more air pollutants selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes and nitrogen oxides.

Below, an example of preparation of a composition containing the gondre extract of the present invention will be described, but the present invention is not intended to limit it, but only to specifically describe it.

Preparation Example 1. Manufacturing of cosmetics

Preparation Example 1-1. Manufacturing of nutritional lotion (milk lotion)

Nutrient lotion containing Gondre extract in the components and contents shown in Table 5 below was prepared according to a conventional method.

Ingredients Preparation Example 1-1
(weight%) Gondre extract of Example 1 50 µg/mL squalane 5.0 beeswax 4.0 Polysorbate 60 1.5 Sorbitan Sesquioleate 1.5 liquid paraffin 0.5 Caprylic/Capric Triglyceride 5.0 glycerin 3.0 Butylene Glycol 3.0 propylene glycol 3.0 carboxyvinyl polymer 0.1 triethanolamine 0.2 Preservatives, Colors, Flavors Appropriate amount Purified water to 100

Preparation Example 1-2. Manufacture of softening lotion (skin lotion)

Softening lotion containing gondre extract in the components and contents shown in Table 6 below was prepared according to a conventional method.

Ingredients Production Example 1-2
(weight%) Gondre extract of Example 1 50 µg/mL glycerin 3.0 Butylene Glycol 2.0 propylene glycol 2.0 carboxyvinyl polymer 0.1 PEG 12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 triethanolamine 0.1 Preservatives, Colors, Flavors Appropriate amount Purified water to 100

Preparation Example 1-3. Manufacture of nutritious cream

A nutritious cream containing the ingredients and contents of Gondre extract in Table 7 below was prepared according to a conventional method.

Ingredients Production Example 1-3
(weight%) Gondre extract of Example 1 50 µg/mL Polysorbate 60 1.5 Sorbitan Sesquioleate 0.5 PEG60 hydrogenated castor oil 2.0 liquid paraffin 10 squalane 5.0 Caprylic/Capric Triglyceride 5.0 glycerin 5.0 Butylene Glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 antiseptic Appropriate amount pigment Appropriate amount Spices Appropriate amount Purified water to 100

Preparation Example 1-4. Manufacture of massage cream

A massage cream containing the ingredients and contents of Gondre extract in Table 8 below was prepared according to a conventional method.

Ingredients Production Example 1-4
(weight%) Gondre extract of Example 1 50 µg/mL beeswax 10.0 Polysorbate 60 1.5 PEG 60 hydrogenated castor oil 2.0 Sorbitan Sesquioleate 0.8 liquid paraffin 40.0 squalane 5.0 Caprylic/Capric Triglyceride 4.0 glycerin 5.0 Butylene Glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, Colors, Flavors Appropriate amount Purified water to 100

Preparation Example 1-5. Manufacture of emulsion for mask pack

An emulsion for mask pack containing gondre extract in the ingredients and contents shown in Table 9 below was prepared according to a conventional method.

Ingredients Production Example 1-5
(weight%) Gondre extract of Example 1 50 µg/mL polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 allantoin 0.1 ethanol 6.0 PEG 12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservatives, Colors, Flavors Appropriate amount Purified water to 100

Manufacturing Example 2. Manufacture of health functional food

Preparation Example 2-1. manufacture of powders

Gondre extract of Example 1 20 mg

Lactose 100 mg

Talc 10 mg

A powder is prepared by mixing the above ingredients and filling them into airtight bags.

Preparation Example 2-2. manufacture of tablets

Gondre extract 10 mg

Corn Starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above ingredients, tablets are prepared by tableting according to a conventional tablet manufacturing method.

Preparation Example 2-3. Manufacture of capsules

Gondre extract of Example 1 10 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium stearate 0.2 mg

Capsules are prepared by mixing the above ingredients and filling them into gelatin capsules according to a conventional capsule preparation method.

Preparation Example 2-4. Manufacture of granules

1,000 mg of the gondre extract of Example 1

Appropriate amount of vitamin mixture

Vitamin A Acetate 70 μg

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

Vitamin B12 0.2 μg

Vitamin C 10 mg

10 μg of biotin

Nicotinamide 1.7 mg

Folic acid 50 μg

Calcium Pantothenate 0.5 mg

Appropriate amount of mineral mixture

Ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium Carbonate 25.3 mg

Potassium Phosphate Monobasic 15 mg

Dibasic Calcium Phosphate 55 mg

Potassium citrate 90 mg

Calcium Carbonate 100 mg

Magnesium Chloride 24.8 mg

The composition ratio of the above vitamin and mineral mixture is a mixture of ingredients suitable for health functional food in a preferred embodiment, but the mixing ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health functional food manufacturing method. Next, granules can be prepared and used in the preparation of health functional food compositions according to conventional methods.

Preparation Example 2-5. Manufacture of beverage formulations

1,000 mg of the gondre extract of Example 1

Citric Acid 1,000 mg

100 g of oligosaccharides

2 g plum concentrate

1 g of taurine

Add purified water to total 900 mL

After mixing the above ingredients according to the usual beverage manufacturing method, stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered and collected in a sterilized 2 L container, sealed and sterilized, and then refrigerated according to the present invention. It is used for the preparation of functional beverage compositions.

Although the present invention has been described in terms of the preferred embodiments mentioned above, various modifications and variations are possible without departing from the spirit and scope of the invention. The appended claims also cover such modifications and variations as fall within the subject matter of this invention.

Claims (9)

Contains gondre extract as an active ingredient,
A composition for skin anti-pollution, having an anti-skin antifouling effect against at least one pollutant selected from the group consisting of polycyclic aromatic hydrocarbons, aldehydes and nitrogen oxides. According to claim 1,
The gondre extract is
A composition for skin anti-pollution, characterized in that extracted with water, lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof. According to claim 1,
The gondre extract is irradiated with ultraviolet UVB at 350 to 1000 mJ/cm ² before or after the gondre is extracted, characterized in that, the skin anti-pollution composition. According to claim 1,
The polycyclic aromatic hydrocarbons include benzopyrene, the nitrogen oxides include ammonium nitrate, and the aldehydes include formaldehyde, skin anti-pollution composition. According to claim 1,
The composition is a composition for skin anti-pollution, characterized in that to prevent skin cell death or inhibition of skin cell proliferation by the contaminant. According to claim 1,
The composition is a composition for skin anti-pollution, characterized in that to alleviate skin irritation or skin inflammation caused by the contaminant. According to claim 1,
The composition is characterized in that the composition for protecting skin cells or the composition for inhibiting skin cell death against the contaminants, the composition for skin anti-pollution. According to claim 1,
The composition is a composition for skin anti-pollution, characterized in that to reduce the activity of XRE (Xenobiotic Response Element) overexpressed by the contaminant. According to claim 1,
The composition for skin anti-pollution is characterized in that the cosmetic composition or health functional food composition, the composition for skin anti-pollution.