KR102553522B1 - Functional composition for anti-pollution and anti-wrinkle with skin aging improvement - Google Patents

Abstract

The present invention relates to a functional composition for anti-wrinkle and anti-pollution containing an extract of sagebrush. According to the present invention, it is possible to provide a composition exhibiting an anti-pollution effect along with anti-wrinkle and anti-aging activities, while containing an extract of horsetail rhizome as an active ingredient. In particular, in the case of the present invention, there is no problem of side effects due to long-term use by utilizing ingredients derived from natural products without cytotoxicity, and various formulations that can utilize its unique functionality Provided as products such as food, pharmaceutical composition, or cosmetic can do.

Description

Functional composition for anti-pollution and skin wrinkle improvement with skin aging improvement effect containing snow horseback riding extract

The present invention relates to a functional composition for anti-pollution and skin wrinkle improvement, which has an effect of improving skin aging, containing an extract of sagebrush. More specifically, it relates to a functional composition showing an anti-pollution effect along with anti-wrinkle and anti-aging activities, while containing an extract of horsetail as an active ingredient.

Aging of skin is largely classified into intrinsic-aging and extrinsic-aging. Intrinsic aging is aging caused by unavoidable genetic factors over time, and its clinical features are mild, such as increased fine wrinkles, decreased elasticity, and dry skin, compared to photoaging. On the other hand, extrinsic aging such as photoaging due to exposure to ultraviolet rays causes thick and deep wrinkles, telangiectasia, and increased irregular pigmentation, making the skin rough, dry, and losing elasticity.

In particular, women in their 40s to 60s experience increased skin roughness, dryness, fine wrinkles and pigmentation, and symptoms such as facial flushing and sweating due to estrogen deficiency. In addition, changes in gastrointestinal function due to aging other than skin aging include decreased secretion of gastric juice, atrophy of the stomach wall, and decreased absorption of nutrients due to reduced secretion of digestive enzymes in the intestine. Accordingly, studies using natural products that do not cause side effects due to long-term use are being actively conducted. However, there are few research reports on aging regulators using natural products, and it is necessary to discover various materials for this.

On the other hand, the skin is a thin film that surrounds the outside of the body, and functions to protect the human body from physical and chemical stimuli of the external environment, and prevents excessive penetration of pollutants from the outside while suppressing evaporation of moisture in the body. As the use of chemicals has recently increased, the skin is exposed to environmental pollutants such as polycyclic aromatic hydrocarbons, aldehydes, nitrogen oxides, nitrates, and fine dust generated during the manufacturing, use, and disposal of these substances, resulting in erythema, edema, The incidence of mild skin troubles such as itching, hives, and inflammation and skin diseases such as atopy, psoriasis, and skin cancer is increasing.

Therefore, in order to protect skin cells from external stress and prevent aging, it is important to suppress oxidative stress caused by active oxygen and strengthen antioxidant defense that can efficiently remove active oxygen. Research on natural substances with antioxidant power is being actively conducted.

Currently, superoxide dismutase (SOD) and catalase, which are antioxidant enzymes that remove reactive oxygen species, and antioxidants, such as vitamin C and vitamin E, are reported to inhibit skin aging and skin cancer. In addition, ingredients used as ingredients for beauty and food are hyaluronic acid, grape seed extract, isoflavone, collagen, ceramide, coenzyme Q10, chondroitin, lycopene, alpha lipoic acid, etc., and their skin beauty effects are mainly due to their antioxidant function ( Jangseo Park, Food Science and Industry, 40, 19 (2007)).

On the other hand, the skin is a body part directly exposed to the external environment, and serves as a protective film to protect important organs of our body, as well as to control moisture evaporation and protect the body from external infections. However, no matter how much the skin blocks virus penetration from the outside, stress from the outside, such as excessive ultraviolet rays and a polluted environment, causes skin irritation and eventually leads to skin aging.

Fine dust refers to small dust with a diameter of 10 μm or less that is invisible to the naked eye as atmospheric suspended matter with various components, and contains toxic substances such as aldehydes, sulfates, and nitrates. In addition, fine dust is produced by a photochemical reaction in the air of yellow dust, automobile soot, and heavy metals emitted from factory chimneys. When exposed to a high-concentration fine dust environment, coughing, eye irritation, skin trouble, and lung inflammation occur. Benzopyrene is exemplified as belonging to the above-mentioned pollutants. The benzopyrene is produced due to incomplete combustion such as automobile exhaust gas or cigarette smoke, and is a toxic substance that causes cell damage through metabolic conversion to epoxide and reaction with DNA. Recently, as the news that it causes atopic dermatitis is encountered, adverse effects on the skin are revealed, and it is one of the dangerous substances that can cause skin deterioration due to problems such as cell mutation and cell damage.

In addition, nitrogen oxides belong to the above-mentioned pollutants. The nitrogen oxide is a compound composed of nitrogen and oxygen, and includes nitrogen monoxide, nitrogen dioxide, dinitrogen monoxide, dinitrogen trioxide, dinitrogen tetroxide, dinitrogen pentoxide, and the like, and ionized products thereof. In general, nitrogen gas is stable and it is difficult for the atoms constituting it to be split into two, but the bond can be broken when a very high temperature is applied. Often, high temperature is formed inside the engine of a vehicle, so that the exhaust gas of the vehicle contains a large amount of nitrogen oxides.

The above-mentioned nitrogen oxide may include ammonium or a salt thereof. Nitric acid, another form of nitrogen oxide, reacts with water to form nitric acid and is also responsible for acid rain. The nitric acid combines with ammonium, an alkaline substance in the air, to produce ammonium nitrate, and the ammonium nitrate is a particulate matter in the air, which is secondary fine dust and adversely affects the human body. When the ammonium nitrate comes into contact with the human body, it causes inflammation in the eyes, nasal mucosa and skin.

In addition, formaldehyde belongs to the above-mentioned pollutants. The formaldehyde is a colorless gas with a strong irritating odor at room temperature and is also called methaneal. The formaldehyde is easily produced when materials containing carbon are incompletely burned, and is synthesized when sunlight and oxygen are applied to hydrocarbons such as methane in the air. The formaldehyde is a skin allergen, and when it comes into contact with the skin, it can cause problems such as skin rash, skin irritation, and dermatitis.

The above-described contaminants cause direct inflammation or necrosis when in contact with the skin at high concentrations, and even residual amounts in the air cause continuous stress on the skin when continuously contacted, thus acting as a cause of skin aging.

Compared to the effects of the above pollutants on the skin, the public's awareness of the risks is still low, but as issues such as fine dust have recently emerged, the risks are gradually attracting attention, and the number of people who are gradually aware of the dangers of pollutants is increasing. It is increasing.

In addition, demand for the term Anti-pollution and these functional products is increasing as a compound word meaning a product that protects the skin from external harmful environments such as fine dust due to various pollutants and fine dust warnings. In particular, in the field of cosmetics, anti-pollution cosmetics are attracting attention. According to the Chinese market research company QY Research report, the anti-pollution skin care market for harmful environments including fine dust is expected to expand to 10 billion dollars by 2025. Interest is growing day by day.

On the other hand, compositions capable of protecting the skin from these contaminants have been studied, but so far, their diversity is lacking, the effect is not clear, and they are often made of difficult-to-manufacture or difficult-to-obtain materials, so they can be easily obtained from the surroundings. There is a need for the development of novel compositions with

KR 10-2169163 B1 KR 10-2078670 B1

An object of the present invention is to provide a functional composition capable of exhibiting anti-aging and skin wrinkle improvement activities by utilizing ingredients derived from natural products.

An object of the present invention is to provide a functional composition capable of exhibiting antioxidant and anti-pollution effects by utilizing ingredients derived from natural products.

In addition, the present invention is to provide the functional composition in various food, cosmetic formulations, etc., so that the effect of a functional product can be provided without problems of side effects due to long-term use as a natural ingredient derived from a plant extract.

In order to achieve the above object, the composition for improving skin wrinkles according to an embodiment of the present invention may be one containing an extract of snow sage horse.

The composition for anti-pollution according to another embodiment of the present invention may include an extract of sagebrush.

Antioxidant and anti-inflammatory composition according to another embodiment of the present invention may be one containing the extract of the horsetail snow.

A food according to another embodiment of the present invention may include the composition.

Health functional food according to another embodiment of the present invention may be one containing the above composition.

Cosmetics according to another embodiment of the present invention may include the above composition.

Hereinafter, the present invention will be described in more detail.

A composition for improving skin wrinkles according to another embodiment of the present invention may include an extract of sagebrush.

The snow-capped horse is a perennial herb that grows in alpine regions across the country. The growth environment is a lot of fallen leaves, and it grows naturally in semi-shade or shade. The height is 30 to 100 cm, and the leaves are 3 to 10 cm long and 1 to 6 cm wide, and have glossy long petioles. It is a pinnate compound leaf that splits 2-3 times in a feather-like shape, with a sharp end and serrated edges. Flowers are white, 10 to 30 cm long, unfold in the shape of a fan, bloom from the bottom and go up. Fruits ripen in July or August, are brown, oval, about 0.3 cm long, and have a glow when ripe. It is used for ornamental purposes, and young shoots are used for food.

In the case of including the extract of the horse eye, it is possible to achieve an effect of improving skin wrinkles by inhibiting the production of active oxygen in the skin.

The composition for anti-pollution according to another embodiment of the present invention may include an extract of sagebrush.

Skin resistance to fine dust may be increased when the extract is included.

Antioxidant and anti-inflammatory composition according to another embodiment of the present invention may be one containing the extract of the horsetail snow.

In the case of including the extract of the horsetail extract, it is possible to prevent and improve inflammatory diseases by inhibiting the activity of inflammation-inducing factors together with antioxidant activity through suppression of reactive oxygen species.

The solvent of the extract may be any one selected from the group consisting of water, alcohol having 1 to 10 carbon atoms, and mixtures thereof.

Preferably, the solvent may be any one selected from the group consisting of water, ethanol, and mixtures thereof. In the case of using the solvent, it may be advantageous to obtain the active ingredient desired in the present invention, so that excellent activity may be exhibited with a small amount of extract.

Preferably, the composition may further include any one selected from the group consisting of lotus flower extract, elegans extract, noni extract, chazugi extract, and mixtures thereof.

Preferably, the composition may further include a lotus flower extract, an extract of shoots, a noni extract, and an extract of Chazugi. In the case of using the above composition, effects that cannot be found in a mixture of different extracts may be exhibited. That is, a synergistic effect may appear due to the interaction with the active ingredient contained in the extract of the horsetail extract.

More preferably, the composition includes a snow horse riding extract, 1 to 5 parts by weight of lotus flower extract based on 100 parts by weight of the snow riding horse extract; 10 to 30 parts by weight of elm extract; It may include 1 to 5 parts by weight of noni extract and 5 to 15 parts by weight of chazuki extract. In the case of the above range, the synergistic effect due to the interaction can greatly increase the anti-aging activity, wrinkle improvement, anti-pollution effect, and antioxidant and anti-inflammatory activity compared to the case of using the extract alone or in simple combination. . In particular, when the mixed composition exhibits a synergistic effect that cannot be confirmed in a mixed composition with different extracts, it is a major feature that a synergistic effect is exhibited by the interaction in the mixing range.

A pharmaceutical composition according to another embodiment of the present invention may include the above composition.

The pharmaceutical composition, in addition to the above composition, various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like may be included, but may not be limited thereto. These components may be used independently or in combination. The content of the additional component may be added in the range of 0.1 part by weight to 20 parts by weight per 100 parts by weight of the natural product complex extract.

A food according to another embodiment of the present invention may include the composition.

'Food' as used in the present invention includes not only general foods and beverages, but also functional foods or health functional foods.

At this time, the amount of the extract in the food or beverage may be added at about 0.01 to 15% by weight of the total food weight, and the health drink composition may be added at a rate of about 0.01 to 10g based on 100 ml, but is not limited thereto .

The above foods are Schisandra extract, strawberry extract, acai berry extract, aronia extract, blueberry extract, cranberry extract, grapefruit extract, orange extract, lemon extract, chocolate mint extract, basil extract, peppermint extract, sage extract, lavender extract, parsley It may further include any one selected from the group consisting of an extract, a gompi extract, a windbreak extract, a coltsfoot extract, a wild flower extract, a noni extract, and a mixture thereof.

The food includes general food, processed food, health functional food, and functional food, and exemplarily, the formulation for the food may be applied to beverages, tea, tablets, meat processed foods, snacks, instant foods, etc. .

When the food composition of the present invention is a beverage composition, there is no particular limitation on other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and it may contain various flavoring agents or natural carbohydrates as additional ingredients like conventional beverages. can Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used, but are not limited thereto. The ratio of the natural carbohydrates may generally range from about 1 to about 20 g per 100 ml of the composition of the present invention, but may not be limited thereto.

In addition, the composition of the present invention may contain fruit flesh for preparing natural fruit juice, fruit juice beverages, and vegetable beverages.

"Functional food" as referred to in the present invention refers to a food manufactured and processed by extracting, concentrating, refining, mixing, etc., a specific ingredient as a raw material or a specific ingredient contained in a food raw material for the purpose of health supplement, including health supplement food. In addition, it is a food designed and processed to sufficiently exert the biological control functions of food components such as bio-defense, regulation of biorhythm, prevention and recovery of disease, and functions related to disease prevention and recovery. It is assumed to include all that also has.

The health functional food may additionally contain food additives, and the suitability as a "food additive" is determined according to the general rules of the Food Additive Code and general test methods approved by the Korea Food and Drug Administration unless otherwise specified. and judged by criteria.

Examples of items listed in the “Food Additives Codex” include chemical synthetic products such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, natural additives such as dark pigment, plant extracts, crystalline cellulose, high-cold pigment, and guar gum, L -Mixed preparations such as sodium glutamate preparations, noodle-added alkali preparations, preservative preparations, and tar color preparations.

Carriers, excipients and diluents that may be included in the functional food or health functional food include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations contain at least one excipient, for example, starch, calcium carbonate, It is prepared by mixing sucrose or lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, solutions for internal use, emulsions, syrups, etc., and various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included in addition to water and liquid paraffin, which are commonly used simple diluents. there is. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for the suppository, witepsol, macrogol, tween 61, cacao butter, laurin fat, glycerogeratin and the like may be used.

Preferably, the food or health functional food is 0.1 to 1 part by weight of Schizandra chinensis based on 100 parts by weight of the extract of snowcapped horsetail; 0.1 to 1 part by weight of cranberry extract; It may further include 0.1 to 2 parts by weight of peppermint extract, 1 to 3 parts by weight of parsley extract, and 0.1 to 1 part by weight of wild flower extract. In the case of the above range, it is possible to provide a food composition with a palatable texture along with a fairly deep and refreshing fragrance while neutralizing the low palatable fragrance and taste of the extract of the horsetail extract. In particular, in the case of the above range, it is included in less than 10% by weight compared to the snow horse extract, and as described above without compromising the functionality of the snow horse horse extract. By improving the taste and flavor of the snow horse horse extract, it is a highly palatable food composition. can provide In addition, the above-mentioned gompi extract; Even in the case of adding Bangpungnamul extract and Chazugi extract, the taste and aroma are excellent and the palatability is high, and the activity of the active ingredient desired in the present invention can be maintained to the maximum.

Cosmetics according to another embodiment of the present invention may include the above composition.

'Cosmetics' as used in the present invention is not limited to cosmetics, and is defined to include all pharmaceuticals or quasi-drugs for external use.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the field, for example, solution, suspension, paste, gel, cream, lotion, powder, soap, surfactant-containing cleanser, oil , Powder foundation, emulsion foundation, wax foundation, and may be formulated as a spray, but is not limited thereto. More specifically, softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder formulation, oil-in-water (O/W) type or water-in-oil (W/O) type formulation or ointment.

The cosmetic may be prepared in the form of an adjuvant that can be applied topically or systemically, which is commonly used in the art, by containing a dermatologically acceptable medium or base in addition to the extract of the horsetail horsetail of the present invention.

Suitable cosmetic formulations include, for example, solutions, gels, solid or pasty anhydrous products, emulsions obtained by dispersing an oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes), non-ionic forms. It may be provided in the form of a follicular dispersion, cream, toner, lotion, powder, ointment, spray or conceal stick. In addition, it can be prepared in the form of a foam (foam) or the form of an aerosol composition further containing a compressed propellant.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as astringent lotion, softening lotion, nutrient lotion, various creams, essences, packs, foundations, etc., and cleansing, face wash, soap, treatment or beauty essence etc. Specific formulations include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, essence, nutrient essence, pack, soap, shampoo, and cleansing foam. , cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, or eye shadow.

The present invention provides a functional composition capable of exhibiting anti-aging and skin wrinkle improvement activities by utilizing ingredients derived from natural products.

The present invention provides a functional composition capable of exhibiting antioxidant and anti-pollution effects by utilizing ingredients derived from natural products.

In addition, the present invention provides the functional composition as a variety of foods, pharmaceutical compositions, cosmetic formulations, etc., so that the effects of functional products can be provided without problems of side effects due to long-term use as natural ingredients derived from plant extracts.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

[Preparation Example: Preparation of extract]

1. Manufacture of snow sage horse extract

The snow sage was washed and cut to a certain size to prepare a snow sage extract (KJB) according to the hot water extraction method.

2. Preparation of other plant extracts

Lotus extract (AD1) according to the hot water extraction method as a material of the composite extract to confirm the synergistic effect on the snow horse riding extract (KJB); Artichoke extract (AD2); Noni extract (AD3) and chazuki extract (AD4) were prepared. In addition, Schisandra chinensis extract (E1), cranberry extract (E2), peppermint extract (E3), parsley extract (E4), and wild chive extract (E5) were prepared in the same manner.

3. Preparation of Mixed Extracts

In order to confirm the effect characteristics according to the mixing of the extract, a mixed extract was prepared with the composition shown in [Table 1] below.

M1 M2 M3 M4 M5 T1 T2 T3 T4 T5 KJB 100 100 100 100 100 100 100 100 100 T6 AD1 0.1 One 3 5 10 - - - - 100 AD2 One 10 15 30 40 - - - - 3 AD3 0.1 One 3 5 10 - - - - 15 AD4 One 5 10 15 20 - - - - 3 E1 - - - - - 0.01 0.1 One 3 10 E2 - - - - - 0.1 One 3 5 One E3 - - - - - 0.01 0.1 2 5 3 E4 - - - - - One One 3 5 2 E5 - - - - - 0.1 0.1 2 5 3

(unit: parts by weight)

[Experimental Example: Activity Evaluation for Preparation Example]

1. Anti-aging and anti-wrinkle activity evaluation

As a cytoprotective effect of CCE against UVB-induced apoptosis, the improvement effect on skin wrinkles was evaluated. To this end, MTT assay analysis was performed on cell viability in HaCaT cells after UVB exposure. UVB was treated with 290 ~ 320nm, 30 min, 3 times, and for objective comparison, the group treated with UVB without treatment of the preparation example was used as a control, and the survival rate compared to the control compared to the cell viability (100%) of the control was evaluated. The results are shown in [Table 2] below.

Con KJB M1 M2 M3 M4 M5 Cell viability according to UVB treatment (%) 100 130 128 142 152 147 133

Referring to [Table 2], it can be confirmed that the anti-aging effect is excellent due to the cytoprotective activity in the case of the preparation example. In addition, in the case of the mixed composition, it can be confirmed that an additional synergistic effect appears when the M2 to M4 are mixed. In particular, referring to M1 and M5, it can be confirmed that in the case of the mixed composition, additional synergistic activity appears only at a certain composition ratio.

Therefore, in the case of the present invention, it can be confirmed that the skin wrinkle improvement effect due to photoaging can appear, and in the case of using the optional mixed composition, additional synergistic activity can be exhibited.

2. Assessment of anti-pollution activity

(1) Evaluation of resistance activity to hydrogen peroxide

HaCaT cells were treated with the untreated control and each preparation to be classified into groups, treated with hydrogen peroxide for each group, and MTT assay was performed to confirm the effect of oxidative stress on the induced HaCaT cells. The evaluation of resistance activity to hydrogen peroxide was evaluated by comparing each manufacturing example with the control for each treated group. The higher the index, the better the resistance. The results are shown in [Table 3] below.

(2) Evaluation of resistance activity against formaldehyde

HaCaT cells were treated with formaldehyde. At the same time, the group treated with nothing was treated as a control, and the groups treated with each preparation were differentiated and cultured. Thereafter, MTT solution was added, cultured, and then treated with DMSO, and absorbance was measured to evaluate the cell viability of each example. For objective comparison, the control result was fixed as an index of 1, and each result was compared with the above control result and evaluated as an index of 1 to 10. The higher the index, the better the resistance. The results are shown in [Table 3] below.

(3) Evaluation of skin resistance to aromatic compounds

After washing the silicon specimen, purified water (control) and each example mixed with purified water were applied, and Naphtalene, Acenaphthylene, Acenaphthene, Fluorene, and Benzopyrene were applied to the silicon specimen. (Benzopyrene) was immersed in a solution containing 500 ppm and then dried at low temperature. Then, the contents of naphthalene, acenathylene, acenaphthene, fluorene, and benzopyrene remaining in each specimen were analyzed and compared with the control. For objective evaluation, the control result was fixed as 1, and the results according to each manufacturing example were evaluated with an index of 1 to 10. The higher the index, the better the resistance. The results are shown in [Table 3] below.

(4) Analysis of test results

Referring to [Table 3] below, it can be seen that KJB exhibits uniform resistance to hydrogen peroxide, formaldehyde, and aromatic compounds. In particular, it was confirmed that a synergistic effect appeared in the case of the M2 to M4 mixed composition, and when outside the above range, the resistance activity was lowered compared to the single extract or the synergistic effect due to mixing at an equal level was not observed. .

Through this, it can be understood that in the case of the extract, when a specific composition is mixed in a specific range, a synergistic effect according to the interaction is exhibited, so that the resistance activity against each harmful substance is increased.

Con KJB M1 M2 M3 M4 M5 Anti-hydrogen peroxide One 2 3 5 6 5 3 Anti-Formaldehyde One 3 3 4 5 6 2 ANT-Aromatic compounds One 3 3 5 6 6 3

3. DPPH radical analysis

Measure the radical scavenging ability of KJB and M1 to M5 using 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical to analyze active oxygen species such as DPPH and superoxide anion related to oxidative stress in vivo did After reacting by adding DPPH solution to KJB and M1 to M5, absorbance was measured. The DPPH radical content was shown in [Table 4] by converting the absorbance ratio of the sample addition group and the control group into % values.

KJB M1 M2 M3 M4 M5 DPPH radical (%) 59 65 47 41 46 58

4. Reduction power analysis

Superoxide anion radical scavenging activity was performed according to the Fridovich method. To 1 ml of the sample, 200 mM phosphate buffer at pH 6.6 and 1 ml of potassium ferricyanide were sequentially added and reacted. Here, a 10% TCA (trichloroacetic acid) solution was mixed with distilled water and ferric chloride in the supernatant, and then the absorbance was measured. KJB and M1 to M5 are shown in [Table 5] below by converting the absorbance ratio into a % value based on the value of the control group.

KJB M1 M2 M3 M4 M5 Superoxide anion (%) 245 258 345 364 352 276

5. DNA damage analysis by hydroxyl radical

Genomic DNA was extracted from PC12 cells according to standard procedures with minor modifications. KJB and M1 to M5, 200 µM FeSO4, 1 mM H2O2, and 50 µg/ml genomic DNA were added to the DNA solution. After completion of the reaction, electrophoresis was performed. After dyeing, it was observed using LAS3000 image analyzer with UV, and the results were expressed in % based on the untreated group and shown in [Table 6] below, and the lower the % value, the better.

KJB M1 M2 M3 M4 M5 DNA oxidation (%) 71 69 53 51 53 64

6. Evaluation of anti-inflammatory activity

HaCaT cells divided into the group treated with the preparation example and the group not treated with anything (control) were treated with microparticles PM10 and the production of the inflammatory mediator prostaglandin E2 was analyzed to suppress inflammation induced by fine dust. was tested. The evaluation results were compared to the control and evaluated, the index of the control was fixed to 1, and the results for each example were compared and evaluated with an index of 1 to 10. The results are shown in [Table 7] below. The higher the index, the better the resistance activity against inflammation caused by the microparticles.

Con KJB M1 M2 M3 M4 M5 Anti-inflammatory activity One 2 2 6 6 6 3

(Unit: index)

Referring to the above [Table 7], it can be confirmed that KJB exhibits excellent antioxidant activity and excellent inhibitory activity against inflammation induced by fine dust. In addition, in the case of the mixed extract, it can be confirmed that a synergistic effect appears when the M2 to M4 mixed composition is used.

Therefore, it can be confirmed that when KJB is used alone or in a mixed composition according to the range of M2 to M4, excellent functional food or external preparation can be provided.

[Experimental Example 3: Evaluation of palatability]

A tea beverage was prepared according to the composition of KJB and T1 to T5, and 10 testers were asked to taste it, and then the aroma and taste were evaluated to evaluate the preference on a scale of 1 to 10. After synthesizing and averaging the results, they are shown in [Table 8] below. In the following indices, the higher the number, the better the degree of preference (rounding was applied to the average value for each index).

KJB T1 T2 T3 T4 T5 palatability 3 2 6 6 3 6

(Unit: index)

As a result of the evaluation of palatability, it was confirmed that most of the tasters showed a low evaluation score in the overall palatability evaluation because of the bitter and strong scent of the extract of Snowcap. However, in the case of the mixed composition of T2 and T3, it was confirmed that the overall taste and aroma were excellent while neutralizing the overall sweet and sweet flavor and the unique flavor of snow horse riding.

In particular, referring to T5, since the mixture of E1 to E5 is included in an amount of less than 10% by weight with respect to the extract of the extract of E1 to E5, the palatability may be excellent while maximally maintaining the activity according to the active ingredient of the extract. In addition, it can be seen that in the case of the above composition, the effect of improving the palatability can be produced even in the composition for M3.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also made according to the present invention. falls within the scope of the rights of

Claims (6)

Containing the extract of snow-capped horsetail,
Based on 100 parts by weight of the snow horse riding extract, 1 to 5 parts by weight of lotus extract, 10 to 30 parts by weight of Aralia extract, 1 to 5 parts by weight of Noni extract and 5 to 15 parts by weight of Chazuki extract
A composition for improving skin wrinkles. Containing the extract of snow-capped horsetail,
Based on 100 parts by weight of the snow horse riding extract, 1 to 5 parts by weight of lotus extract, 10 to 30 parts by weight of Aralia extract, 1 to 5 parts by weight of Noni extract and 5 to 15 parts by weight of Chazuki extract
A composition for anti-pollution. Containing the extract of snow-capped horsetail,
Based on 100 parts by weight of the snow horse riding extract, 1 to 5 parts by weight of lotus extract, 10 to 30 parts by weight of Aralia extract, 1 to 5 parts by weight of Noni extract and 5 to 15 parts by weight of Chazuki extract
Antioxidant and anti-inflammatory composition. A food product comprising the composition according to any one of claims 1 to 3. A health functional food comprising the composition according to any one of claims 1 to 3. A cosmetic comprising the composition according to any one of claims 1 to 3.