KR102522067B1 - 무-세포 메틸화된 dna의 포획 방법 및 이의 이용 - Google Patents

무-세포 메틸화된 dna의 포획 방법 및 이의 이용 Download PDF

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KR102522067B1
KR102522067B1 KR1020217023549A KR20217023549A KR102522067B1 KR 102522067 B1 KR102522067 B1 KR 102522067B1 KR 1020217023549 A KR1020217023549 A KR 1020217023549A KR 20217023549 A KR20217023549 A KR 20217023549A KR 102522067 B1 KR102522067 B1 KR 102522067B1
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dna
nucleic acid
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acid molecules
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KR20210095962A (ko
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드 까르발로 다니엘 디니즈
슈 이 센
라자트 싱하니아
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유니버시티 헬스 네트워크
시나이 헬스 시스템
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KR1020217023549A 2016-05-03 2017-05-03 무-세포 메틸화된 dna의 포획 방법 및 이의 이용 Active KR102522067B1 (ko)

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KR1020237012338A KR20230054746A (ko) 2016-05-03 2017-05-03 무-세포 메틸화된 dna의 포획 방법 및 이의 이용

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US201662331070P 2016-05-03 2016-05-03
US62/331,070 2016-05-03
KR1020187034995A KR102359767B1 (ko) 2016-05-03 2017-05-03 무-세포 메틸화된 dna의 포획 방법 및 이의 이용
PCT/CA2017/000108 WO2017190215A1 (en) 2016-05-03 2017-05-03 Methods of capturing cell-free methylated dna and uses of same

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US (5) US11078475B2 (https=)
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Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9115386B2 (en) 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
KR102522067B1 (ko) * 2016-05-03 2023-04-13 유니버시티 헬스 네트워크 무-세포 메틸화된 dna의 포획 방법 및 이의 이용
MX2019007444A (es) 2016-12-22 2019-08-16 Guardant Health Inc Metodos y sistemas para analisis de moleculas de acido nucleico.
CA3063405A1 (en) * 2017-05-12 2018-11-15 President And Fellows Of Harvard College Universal early cancer diagnostics
US12227737B2 (en) * 2017-07-12 2025-02-18 University Health Network Cancer detection and classification using methylome analysis
BR112020000681A2 (pt) * 2017-07-12 2020-07-14 University Health Network detecção e classificação de cancro utilizando análise de metilome
US20210156863A1 (en) * 2017-11-03 2021-05-27 University Health Network Cancer detection, classification, prognostication, therapy prediction and therapy monitoring using methylome analysis
CN108796057A (zh) * 2018-06-29 2018-11-13 上海交通大学 一种少量样品全基因组dna甲基化的检测方法及试剂盒
CN108796039B (zh) * 2018-06-29 2021-10-08 上海交通大学 一种用于dna甲基化检测的试剂盒与方法及应用
CN109023537B (zh) * 2018-09-04 2021-10-08 上海交通大学 一种微量dna样品高通量测序文库的构建技术
CN109097439A (zh) * 2018-09-04 2018-12-28 上海交通大学 一种检测少量样品全基因组dna甲基化的方法
WO2020160414A1 (en) * 2019-01-31 2020-08-06 Guardant Health, Inc. Compositions and methods for isolating cell-free dna
CN109825559A (zh) * 2019-03-11 2019-05-31 上海交通大学 一种血浆cfDNA全局性甲基化检测方法及应用
US11891653B2 (en) 2019-09-30 2024-02-06 Guardant Health, Inc. Compositions and methods for analyzing cell-free DNA in methylation partitioning assays
CN115087744A (zh) * 2019-11-06 2022-09-20 大学健康网络 用于无细胞MeDIP测序的合成加标对照及其使用方法
EP4617375A3 (en) * 2019-11-26 2025-10-29 Guardant Health, Inc. Methods, compositions and systems for improving the binding of methylated polynucleotides
CN111154846A (zh) * 2020-01-13 2020-05-15 四川大学华西医院 一种甲基化核酸的检测方法
EP4715052A2 (en) * 2020-05-18 2026-03-25 Myriad Women's Health, Inc. Nucleic acid sample enrichment and screening methods
US12592321B2 (en) 2020-06-19 2026-03-31 University Health Network Cancer detection and classification using methylome analysis
KR102332540B1 (ko) 2020-07-02 2021-11-29 의료법인 성광의료재단 다운증후군 특이적인 후성학적 마커를 이용한 다운증후군 진단 방법
KR102332539B1 (ko) 2020-07-02 2021-11-29 의료법인 성광의료재단 다운증후군 진단용 후성학적 바이오마커 조성물 및 이의 용도
EP4585697A3 (en) * 2020-07-30 2025-08-06 Guardant Health, Inc. Methods for isolating cell-free dna
AU2021345359A1 (en) 2020-09-21 2023-05-11 Enumera Molecular, Inc. Compositions and methods for isolation of cell-free dna
EP4222278A1 (en) * 2020-09-30 2023-08-09 Guardant Health, Inc. Compositions and methods for analyzing dna using partitioning and a methylation-dependent nuclease
JP2023547620A (ja) 2020-10-23 2023-11-13 ガーダント ヘルス， インコーポレイテッド 分配および塩基変換を使用してｄｎａを解析するための組成物および方法
JP2024525190A (ja) * 2021-06-18 2024-07-10 シングレラ ジェノミクス （チャンスー） リミテッド 腫瘍評価のための物質及び方法
JP2024523401A (ja) * 2021-06-21 2024-06-28 ガーダント ヘルス， インコーポレイテッド コピー数情報に基づく組織起源分析のための方法および組成物
CN113621691A (zh) * 2021-09-27 2021-11-09 广东省妇幼保健院 基于一代测序技术直接检测KLF1基因启动子区CpG甲基化的方法和试剂盒
CN114045342A (zh) * 2021-12-01 2022-02-15 大连晶泰生物技术有限公司 一种游离DNA(cfDNA)甲基化突变的检测方法及试剂盒
WO2023107709A1 (en) * 2021-12-10 2023-06-15 Adela, Inc. Methods and systems for generating sequencing libraries
WO2023192635A2 (en) * 2022-04-01 2023-10-05 Twist Bioscience Corporation Libraries for methylation analysis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017514499A (ja) 2014-05-09 2017-06-08 ライフコーデックス アーゲー 特別な細胞タイプに由来するｄｎａの検出とそれに関連する方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818762B2 (en) * 2001-05-25 2004-11-16 Maine Molecular Quality Controls, Inc. Compositions and methods relating to nucleic acid reference standards
AU2002363062B2 (en) * 2001-10-09 2007-03-22 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
CN101802223A (zh) * 2007-08-15 2010-08-11 香港大学 用于高通量亚硫酸氢盐dna-测序的方法和组合物及其用途
CN103443338B (zh) * 2011-02-02 2017-09-22 华盛顿大学商业化中心 大规模平行邻接作图
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9732390B2 (en) * 2012-09-20 2017-08-15 The Chinese University Of Hong Kong Non-invasive determination of methylome of fetus or tumor from plasma
SI4056712T1 (sl) * 2012-09-20 2024-10-30 The Chinese University Of Hong Kong Neinvazivno določanje metiloma tumorja iz plazme
US10706957B2 (en) * 2012-09-20 2020-07-07 The Chinese University Of Hong Kong Non-invasive determination of methylome of tumor from plasma
TWI813141B (zh) * 2014-07-18 2023-08-21 香港中文大學 Ｄｎａ混合物中之組織甲基化模式分析
KR102522067B1 (ko) * 2016-05-03 2023-04-13 유니버시티 헬스 네트워크 무-세포 메틸화된 dna의 포획 방법 및 이의 이용
BR112020000681A2 (pt) * 2017-07-12 2020-07-14 University Health Network detecção e classificação de cancro utilizando análise de metilome
US20210156863A1 (en) * 2017-11-03 2021-05-27 University Health Network Cancer detection, classification, prognostication, therapy prediction and therapy monitoring using methylome analysis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017514499A (ja) 2014-05-09 2017-06-08 ライフコーデックス アーゲー 特別な細胞タイプに由来するｄｎａの検出とそれに関連する方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
nature protocols, 2012, Vol.7, No.4, pp.617-636 1부.*

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US11560558B2 (en) 2023-01-24
US20220119796A1 (en) 2022-04-21
US20220073902A1 (en) 2022-03-10
BR112018072536A2 (pt) 2019-03-26
US20230287384A1 (en) 2023-09-14
EP4425178A2 (en) 2024-09-04
JP7434215B2 (ja) 2024-02-20
JP2021168657A (ja) 2021-10-28
KR20210095962A (ko) 2021-08-03
JP2019521315A (ja) 2019-07-25
EP3452615A1 (en) 2019-03-13
CA3022606A1 (en) 2017-11-09
EP3452615A4 (en) 2019-12-04
US20250313825A1 (en) 2025-10-09
CN109415763A (zh) 2019-03-01
KR20190003987A (ko) 2019-01-10
US20190144848A1 (en) 2019-05-16
JP2024054242A (ja) 2024-04-16
WO2017190215A1 (en) 2017-11-09
US11078475B2 (en) 2021-08-03
KR102359767B1 (ko) 2022-02-08
KR20230054746A (ko) 2023-04-25
JP6979554B2 (ja) 2021-12-15
EP4425178A3 (en) 2024-12-18

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