KR102504801B1 - Manufacturing method of feed composition for water quality improvement using effective microorganisms - Google Patents

Manufacturing method of feed composition for water quality improvement using effective microorganisms Download PDF

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KR102504801B1
KR102504801B1 KR1020220128411A KR20220128411A KR102504801B1 KR 102504801 B1 KR102504801 B1 KR 102504801B1 KR 1020220128411 A KR1020220128411 A KR 1020220128411A KR 20220128411 A KR20220128411 A KR 20220128411A KR 102504801 B1 KR102504801 B1 KR 102504801B1
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mixture
parts
water quality
feed composition
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이동훈
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이동훈
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/28Silicates, e.g. perlites, zeolites or bentonites
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K30/00Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/20Dehydration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/25Shaping or working-up of animal feeding-stuffs by extrusion
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/30Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
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  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Inorganic Chemistry (AREA)
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  • Marine Sciences & Fisheries (AREA)
  • Fodder In General (AREA)

Abstract

The present invention relates to a method for manufacturing a feed composition for improving water quality using effective microorganisms. More specifically, the method comprises: a raw material mixing step of mixing fish powder, wheat flour, starch, peeled soybean meal, sesame dregs, corn, and micronutrients; a first drying step of drying the mixture prepared through the raw material mixing step; a microbial fermentation step of pulverizing the mixture dried through the first drying step, and mixing the mixture and a mixed solution of useful microorganisms to ferment the mixture; a second drying step of drying the mixture fermented through the microbial fermentation step; a forming step of pulverizing and forming the mixture dried through the second drying step; and a fish oil coating step of coating fish oil on the surface of the molded product manufactured through the forming step. According to the present invention, the feed composition for improving water quality contains abundant nutrients and is fermented with useful microorganisms, thereby exhibiting excellent water quality improvement effect and preservation properties.

Description

유용미생물을 활용한 수질개선용 사료 조성물의 제조방법 {MANUFACTURING METHOD OF FEED COMPOSITION FOR WATER QUALITY IMPROVEMENT USING EFFECTIVE MICROORGANISMS}Manufacturing method of feed composition for water quality improvement using useful microorganisms {MANUFACTURING METHOD OF FEED COMPOSITION FOR WATER QUALITY IMPROVEMENT USING EFFECTIVE MICROORGANISMS}

본 발명은 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법에 관한 것으로, 더욱 상세하게는 영양성분이 풍부하게 함유되어 있을 뿐만 아니라, 유용미생물로 발효되어 우수한 수질개선 효과와 보존성을 나타내는 사료 조성물을 제조하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법에 관한 것이다.The present invention relates to a method for producing a feed composition for improving water quality using useful microorganisms, and more particularly, to a feed composition that is rich in nutrients and fermented with useful microorganisms to exhibit excellent water quality improvement effects and preservation. It relates to a method for producing a feed composition for improving water quality using useful microorganisms for producing.

전 세계적으로 어분의 수요는 지속적으로 늘어날 것으로 전망되며, 공급의 제한에 따르는 가격의 인상이 예상되는데, 미국에서는 최근에 어분대체품이 개발되어서 국내 동물 및 어종별 이용가능성 실험이 시작되고 있다.Demand for fishmeal is expected to continue to increase worldwide, and price increases are expected due to supply restrictions. In the United States, fishmeal substitutes have recently been developed, and experiments on availability by domestic animals and fish species have begun.

이러한 어분대체품의 개발은 다양한 국가에서 관심을 가지고 진행중이지만, 종래에 개발된 어분대체품은 주로 가축사료를 위한 것으로, 양어, 관상어 등의 사료만을 위한 개발은 아니었으며, 양어, 관상어 등의 먹이 특성을 고려한 어류 사료를 위한 어분대체품의 개발은 중요한 의미를 가지고 있다.Although the development of these fishmeal substitutes is in progress with interest in various countries, conventionally developed fishmeal substitutes are mainly for livestock feed, not only for feed for fish and ornamental fish, etc. The development of fishmeal substitutes for the considered fish feed has important implications.

어분은 어류 사료의 주단백질 사료원으로 각 어종마다 다르지만 현재 국내에서 생산되는 양어사료는 평균 20 내지 30%, 특히 뱀장어 사료는 60 내지 70%의 어분을 사용하고 있는 실정이고, 양어사료와 어분사용량은 빠른 속도로 증가하고 있다.Fishmeal is the main source of protein for fish feed, and it is different for each fish species, but currently, fish feed produced in Korea uses an average of 20 to 30%, especially eel feed, uses 60 to 70% of fishmeal. is increasing at a rapid pace.

또한, 어분대체품에 사용되는 단백질사료원의 다변화는 국내수요에 대처하고 나아가서는 어분 수입의 최소화와 어분대체품에 대한 수출의 가능성을 생각할 때 산업적으로나 경제적으로 중요한 연구과제다.In addition, diversification of protein feed sources used in fishmeal substitutes is an industrially and economically important research task considering the possibility of minimizing fishmeal imports and exporting fishmeal substitutes in order to cope with domestic demand.

그러나, 종래에 어분대체품의 경우 수질오염을 야기하며, 어분을 주성분으로 하기 때문에, 비교적 영양성분이 고르게 분포되어 있지 못할 뿐만 아니라, 쉽게 변질되어 보존성이 낮은 문제점이 있었다.However, conventional fishmeal substitutes cause water pollution, and since fishmeal is used as a main component, nutrients are not evenly distributed, and they are easily deteriorated, resulting in poor preservation.

한국특허등록 제10-0881886호(2009.01.29.)Korean Patent Registration No. 10-0881886 (2009.01.29.) 한국특허등록 제10-2273863호(2021.06.30.)Korean Patent Registration No. 10-2273863 (2021.06.30.)

본 발명의 목적은 영양성분이 풍부하게 함유되어 있을 뿐만 아니라, 유용미생물로 발효되어 우수한 수질개선 효과와 보존성을 나타내는 사료 조성물을 제조하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법을 제공하는 것이다.An object of the present invention is to provide a method for producing a feed composition for improving water quality using useful microorganisms that is fermented with useful microorganisms to produce a feed composition that is rich in nutrients and exhibits excellent water quality improvement effect and preservation will be.

본 발명의 목적은 어분, 소맥분, 전분, 탈피대두박, 깻묵, 옥수수 및 미량영양소를 혼합하는 원료혼합단계, 상기 원료혼합단계를 통해 제조된 혼합물을 건조하는 제1건조단계, 상기 제1건조단계를 통해 건조된 혼합물을 분쇄한 후에 유용미생물 혼합액을 혼합하고 발효하는 미생물발효단계, 상기 미생물발효단계를 통해 발효된 혼합물을 건조하는 제2건조단계, 상기 제2건조단계를 통해 건조된 혼합물을 분쇄한 후에 성형하는 성형단계 및 상기 성형단계를 통해 제조된 성형물의 표면에 어유를 코팅하는 어유코팅단계로 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법을 제공함에 의해 달성된다.An object of the present invention is a raw material mixing step of mixing fish meal, wheat flour, starch, peeled soybean meal, seed cake, corn and micronutrients, a first drying step of drying the mixture prepared through the raw material mixing step, and the first drying step. A microbial fermentation step of mixing and fermenting a mixture of useful microorganisms after pulverizing the mixture dried through the microbial fermentation step, a second drying step of drying the mixture fermented through the microbial fermentation step, and pulverizing the mixture dried through the second drying step. It is achieved by providing a method for producing a feed composition for improving water quality using useful microorganisms, characterized in that it consists of a molding step of molding later and a fish oil coating step of coating fish oil on the surface of the molded product produced through the molding step.

본 발명의 바람직한 특징에 따르면, 상기 원료혼합단계는 어분 100 중량부 소맥분 70 내지 80 중량부, 전분 20 내지 30 중량부, 탈피대두박 45 내지 55 중량부, 깻묵 20 내지 30 중량부, 옥수수 20 내지 30 중량부 및 미량영양소 70 내지 80 중량부로 이루어지는 것으로 한다.According to a preferred feature of the present invention, the raw material mixing step comprises 100 parts by weight of fish meal, 70 to 80 parts by weight of wheat flour, 20 to 30 parts by weight of starch, 45 to 55 parts by weight of hulled soybean meal, 20 to 30 parts by weight of seed meal, and 20 to 30 parts by weight of corn. It shall consist of 70 to 80 parts by weight of parts by weight and micronutrients.

본 발명의 더 바람직한 특징에 따르면, 상기 원료혼합단계에서는 상기 어분 100 중량부 대비 첨가제 15 내지 25 중량부가 더 함유되며, 상기 첨가제는 농축대두단백 및 칠면초로 이루어진 그룹에서 선택된 하나 이상으로 이루어지는 것으로 한다.According to a more preferred feature of the present invention, in the raw material mixing step, 15 to 25 parts by weight of additives are further contained relative to 100 parts by weight of the fish meal, and the additives are made of at least one selected from the group consisting of concentrated soybean protein and chilmyeoncho.

본 발명의 더욱 바람직한 특징에 따르면, 상기 어분은 정어리, 고등어 및 대구가 1:1:1의 중량부로 혼합되어 이루어지는 것으로 한다.According to a more preferred feature of the present invention, the fishmeal is made by mixing sardine, mackerel and cod in a weight part of 1:1:1.

본 발명의 더욱 더 바람직한 특징에 따르면, 상기 미량영양소는 비타민 혼합물, 새우분말, 다시마 및 스피룰리나로 이루어진 그룹에서 선택된 하나 이상으로 이루어지는 것으로 한다.According to a more preferred feature of the present invention, the micronutrient is to consist of at least one selected from the group consisting of vitamin mixture, shrimp powder, kelp and spirulina.

본 발명의 더욱 더 바람직한 특징에 따르면, 상기 미생물발효단계는 상기 제1건조단계를 통해 건조된 혼합물을 분쇄한 후에, 분쇄된 혼합물 100 중량부 대비 유용미생물 혼합액 0.005 내지 0.01 중량부를 혼합하고 35 내지 55℃의 온도에서 10 내지 15시간 동안 발효하여 이루어지는 것으로 한다.According to a more preferred feature of the present invention, in the microbial fermentation step, after pulverizing the mixture dried through the first drying step, 0.005 to 0.01 parts by weight of the mixed solution of useful microorganisms is mixed with 100 parts by weight of the pulverized mixture, and 35 to 55 parts by weight are mixed. It is made by fermentation for 10 to 15 hours at a temperature of ° C.

본 발명의 더욱 더 바람직한 특징에 따르면, 상기 유용미생물 혼합액은 클로렐라, 유산균, 효모균, 고초균 및 황국균으로 이루어지는 것으로 한다.According to a further preferred feature of the present invention, the useful microorganism mixture is made of chlorella, lactic acid bacteria, yeast, Bacillus subtilis and H. ghetto.

본 발명의 더욱 더 바람직한 특징에 따르면, 상기 유용미생물 혼합액은 상기 유용미생물 혼합액은 클로렐라, 유산균, 효모균, 고초균 및 황국균이 1:1:1:1:1의 중량부로 혼합되어 이루어지는 것으로 한다.According to a further preferred feature of the present invention, the useful microbial mixture is made by mixing chlorella, lactic acid bacteria, yeast, Bacillus subtilis and Aspergillus subtilis in a weight part of 1: 1: 1: 1: 1.

본 발명의 더욱 더 바람직한 특징에 따르면, 상기 성형단계는 상기 제2건조단계를 통해 건조된 혼합물을 분쇄한 후에 스크루의 회전속도가 200 내지 300rpm인 압출기에 투입하고 40 내지 50℃의 온도로 압출하여 이루어지는 것으로 한다.According to a more preferred feature of the present invention, the molding step is to grind the mixture dried through the second drying step, then put it into an extruder having a screw rotational speed of 200 to 300 rpm and extrude at a temperature of 40 to 50 ° C. to be made

본 발명의 더욱 더 바람직한 특징에 따르면, 상기 원료혼합단계에서는 상기 어분 100 중량부 대비 광물혼합물 5 내지 20 중량부가 더 함유되며, 상기 광물혼합물은 맥반석, 펄라이트 및 일라이트가 1:1:1의 중량부로 혼합되어 이루어지는 것으로 한다.According to a more preferred feature of the present invention, in the raw material mixing step, 5 to 20 parts by weight of the mineral mixture is further contained relative to 100 parts by weight of the fish meal, and the mineral mixture is elvan, perlite and illite in a weight ratio of 1: 1: 1 It is assumed that it is made by mixing in parts.

본 발명에 따른 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법은 영양성분이 풍부하게 함유되어 있을 뿐만 아니라, 유용미생물로 발효되어 우수한 수질개선 효과와 보존성을 나타내는 사료 조성물을 제공하는 탁월한 효과를 나타낸다.The method for producing a feed composition for improving water quality using useful microorganisms according to the present invention has an excellent effect of providing a feed composition that is fermented with useful microorganisms and exhibits excellent water quality improvement effect and preservation as well as rich in nutrients. indicate

도 1은 본 발명에 따른 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법을 나타낸 순서도이다.1 is a flow chart showing a method for producing a feed composition for improving water quality using useful microorganisms according to the present invention.

이하에는, 본 발명의 바람직한 실시예와 각 성분의 물성을 상세하게 설명하되, 이는 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 발명을 용이하게 실시할 수 있을 정도로 상세하게 설명하기 위한 것이지, 이로 인해 본 발명의 기술적인 사상 및 범주가 한정되는 것을 의미하지는 않는다.Hereinafter, a preferred embodiment of the present invention and the physical properties of each component will be described in detail, but this is to be explained in detail so that a person having ordinary knowledge in the art to which the present invention belongs can easily practice the invention, This is not meant to limit the technical spirit and scope of the present invention.

본 발명에 따른 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법은 어분, 소맥분, 전분, 탈피대두박, 깻묵, 옥수수 및 미량영양소를 혼합하는 원료혼합단계(S101), 상기 원료혼합단계(S101)를 통해 제조된 혼합물을 건조하는 제1건조단계(S103), 상기 제1건조단계(S103)를 통해 건조된 혼합물을 분쇄한 후에 유용미생물 혼합액을 혼합하고 발효하는 미생물발효단계(S105), 상기 미생물발효단계(S105)를 통해 발효된 혼합물을 건조하는 제2건조단계(S107), 상기 제2건조단계(S107)를 통해 건조된 혼합물을 분쇄한 후에 성형하는 성형단계(S109) 및 상기 성형단계(S109)를 통해 제조된 성형물의 표면에 어유를 코팅하는 어유코팅단계(S111)로 이루어진다.The method for producing a feed composition for improving water quality using useful microorganisms according to the present invention includes a raw material mixing step (S101) of mixing fish meal, wheat flour, starch, peeled soybean meal, seed cake, corn and micronutrients, and the raw material mixing step (S101) A first drying step (S103) of drying the mixture prepared through, a microbial fermentation step (S105) of mixing and fermenting a mixture of useful microorganisms after pulverizing the dried mixture through the first drying step (S103), the microorganism A second drying step (S107) of drying the mixture fermented through the fermentation step (S105), a molding step (S109) of pulverizing and molding the mixture dried through the second drying step (S107), and the molding step ( It consists of a fish oil coating step (S111) of coating fish oil on the surface of the molding manufactured through S109).

상기 원료혼합단계(S101)는 어분, 소맥분, 전분, 탈피대두박, 깻묵, 옥수수 및 미량영양소를 혼합하는 단계로, 어분 100 중량부, 소맥분 70 내지 80 중량부, 전분 20 내지 30 중량부, 탈피대두박 45 내지 55 중량부, 깻묵 20 내지 30 중량부, 옥수수 20 내지 30 중량부 및 미량영양소 70 내지 80 중량부를 혼합하여 이루어지는 것이 바람직하다.The raw material mixing step (S101) is a step of mixing fish meal, wheat flour, starch, peeled soybean meal, seed meal, corn, and micronutrients, including 100 parts by weight of fish meal, 70 to 80 parts by weight of wheat flour, 20 to 30 parts by weight of starch, and peeled soybean meal It is preferably made by mixing 45 to 55 parts by weight, 20 to 30 parts by weight of seed meal, 20 to 30 parts by weight of corn, and 70 to 80 parts by weight of micronutrients.

상기 어분은 본 발명을 통해 제조되는 수질개선용 사료 조성물의 주재료가 되는 성분으로, 미국사료협회는 어분을 '기름기 부분을 추출하든 추출하지 않든 아직 썩지 않은 생선을 말려서 간 조직'이라고 규정을 하고 있다.The fishmeal is the main ingredient of the feed composition for water quality improvement produced by the present invention, and the American Feed Association defines fishmeal as 'liver tissue obtained by drying fish that have not yet rotted, whether or not the oily part is extracted.' .

일반적으로, 어분에는 생선의 머리, 꼬리, 내장 및 혈액등이 함유되어 있으며, 보통 육분이나 골분보다 단백질 수치가 높은 특징을 나타내는데, 본 발명에서는 사료 조성물의 기호도와 영양성분을 고려하여 정어리, 고등어 및 대구가 1:1:1의 중량부로 혼합되어 이루어진 어분을 사용하였다.In general, fishmeal contains the head, tail, intestines, and blood of fish, and usually has a higher protein level than meat or bone meal. Fishmeal was used in which cod was mixed in a weight part of 1:1:1.

또한, 상기 미량영양소는 70 내지 80 중량부가 함유되며, 본 발명을 통해 제조되는 수질개선용 사료 조성물에 각종 영양성분 함량을 향상시키는 역할을 하는데, 비타민 혼합물, 새우분말, 다시마 및 스피룰리나로 이루어진 그룹에서 선택된 하나 이상으로 이루어지는 것이 바람직하며, 상기 비타민 혼합물은 비타민 C 및 비타민 A로 이루어지는 것이 바람직하다.In addition, the micronutrients are contained in an amount of 70 to 80 parts by weight, and serve to improve the content of various nutrients in the feed composition for improving water quality prepared by the present invention, in the group consisting of vitamin mixture, shrimp powder, kelp and spirulina. It is preferably composed of one or more selected, and the vitamin mixture is preferably composed of vitamin C and vitamin A.

또한, 본 발명을 통해 제조되는 수질개선용 사료 조성물에는 사료 조성물의 기호도를 증진시키기 위해 향미제나 천연착색제 등이 더 함유될 수도 있는데, 향미제로는 글루타민산나트륨을 사용할 수 있으며, 천연착색제로는 아스타잔틴(astaxanthin) 등을 사용할 수 있다.In addition, the feed composition for improving water quality prepared according to the present invention may further contain a flavoring agent or a natural coloring agent to enhance the preference of the feed composition. tin (astaxanthin) and the like can be used.

또한, 상기 원료혼합단계에서는 상기 어분 100 중량부 대비 첨가제 15 내지 25 중량부가 더 함유될 수 있으며, 상기 첨가제는 농축대두단백 및 칠면초로 이루어진 그룹에서 선택된 하나 이상으로 이루어지는 것이 바람직한데, 농축대두단백과 칠면초가 1:1의 중량부로 혼합되는 것이 더욱 바람직하다.In addition, in the raw material mixing step, 15 to 25 parts by weight of additives may be further contained relative to 100 parts by weight of the fish meal, and the additive is preferably composed of at least one selected from the group consisting of concentrated soybean protein and chilmyeoncho. It is more preferable that the chilmyeoncho is mixed in a weight part of 1:1.

상기 농축대두단백은 어류에 고품질의 단백질을 공급하여 성장을 촉진하는 역할을 하며, 상기 칠면초(七面草, Suaeda japonica)는 한국 및 일본의 바닷가에서 군생하는 한해살이풀로서 DHA를 포함하는 영양성분이 다량 함유되어 있어, 본 발명을 통해 제조되는 수질개선용 사료 조성물에 영양성분 함량을 향상시키는 역할을 한다.The concentrated soybean protein serves to promote growth by supplying high-quality protein to fish, and Suaeda japonica is an annual plant that grows in clusters on the beaches of Korea and Japan, Since it is contained in a large amount, it serves to improve the nutrient content of the feed composition for improving water quality prepared through the present invention.

이때, 상기 칠면초는 칠면초를 80 내지 100℃의 수증기(steam)로 30 내지 3시간 동안 증숙한 후에, 0.5 내지 1.5cm의 크키로 절단된 것을 사용하는 것이 바람직하다.At this time, it is preferable to use the seven-myeoncho after steaming the seven-myeoncho with steam at 80 to 100 ° C. for 30 to 3 hours, and then cutting them to a size of 0.5 to 1.5 cm.

또한, 상기 원료혼합단계(S101)에서는 상기 어분 100 중량부 대비 광물혼합물 5 내지 20 중량부가 더 함유될 수 있는데, 상기 광물혼합물은 맥반석, 펄라이트 및 일라이트가 1:1:1의 중량부로 혼합되어 이루어지는 것이 바람직하다.In addition, in the raw material mixing step (S101), 5 to 20 parts by weight of the mineral mixture may be further contained relative to 100 parts by weight of the fishmeal. It is desirable that

상기와 같이 광물혼합물이 함유되면 본 발명을 통해 제조되는 사료 조성물의 수질개선 효과가 더욱 향상될 수 있는데, 상기 광물혼합물의 함량이 5 중량부 미만이면 상기의 효과가 미미하며, 상기 광물혼합물의 함량이 20 중량부를 초과하게 되면 상기의 효과는 크게 향상되지 않으면서 사료 조성물의 기호도가 저하되기 때문에 바람직하지 못하다.When the mineral mixture is contained as described above, the water quality improvement effect of the feed composition prepared through the present invention can be further improved. If the mineral mixture is less than 5 parts by weight, the above effect is insignificant, and the mineral mixture content When the amount exceeds 20 parts by weight, the above effects are not greatly improved, and the preference of the feed composition is lowered, which is not preferable.

상기 맥반석(quartz porphyry)은 화성암류 중 석영반암에 속하는 암석으로 성분은 알칼리 장석과 석영을 주성분으로 하고 있는데, 화학적 조성은 SiO2 및 Al2O3가 대부분이고 Fe, Mg, Ca, Na, K, Mn, Ge 등이 소량 함유되어 있고, 이러한 미랭 광물질이 어류의 체내에서 유리되어 유용한 역할을 하며, 다공성으로 인해 표면적이 넓기 때문에, 수중에 이물질을 흡착 또는 여과하는 역할을 하여 수질개선 효과를 나타낸다.The elvan (quartz porphyry) is a rock belonging to quartz porphyry among igneous rocks, and its components are mainly composed of alkali feldspar and quartz. The chemical composition is mostly SiO 2 and Al 2 O 3 and Fe, Mg, Ca, Na, K , Mn, Ge, etc. are contained in small amounts, and these mild minerals play a useful role as they are released from the body of fish, and due to their porosity, they have a large surface area, so they absorb or filter foreign substances in the water, showing the effect of improving water quality. .

또한, 상기 펄라이트(Perlite)는 진주암을 760 내지 1,200℃의 고온에서 급격히 가열하여 공극을 많게 한 경석(輕石)으로 무게의 3 내지 4배 정도의 흡수력을 가지고, pH는 6.0 내지 8.0 정도로 중성에 가깝다. 완충능이 높지 못하고 양이온 치환용량(Cation Exchange Capacity) 0.15me/100g로 적다. 반면 무기성분을 함유하지 않고 잘 파되되지 않아 배지의 통기성은 양호한 편인데, 부유물질 제거 기능과 중금속 제거 효과가 뛰어나 수질개선 효과를 나타낸다.In addition, the perlite is a pumice stone in which perlite is rapidly heated at a high temperature of 760 to 1,200 ° C. to increase the number of pores, and has an absorption capacity of about 3 to 4 times its weight, and a neutral pH of about 6.0 to 8.0. close. The buffering capacity is not high and the cation exchange capacity is low at 0.15me/100g. On the other hand, since it does not contain inorganic components and does not break down well, the medium has good air permeability, but it has excellent floating matter removal function and heavy metal removal effect, showing the effect of improving water quality.

또한, 상기 일라이트는 운모의 한 성분으로서 팔면체 양이온은 Al3 +, Mg2 +, Fe2 + 외에도 Li, Cr , Ni, Mn, Ti, Zn, Co, Cu 및 V 등이 함유되어 있으며, 부유물질 제거 기능과 중금속 제거 효과가 뛰어나 수질개선 효과를 나타낸다.In addition, the illite is a component of mica, and the octahedral cation contains Al 3+ , Mg 2+ , Fe 2+ as well as Li, Cr, Ni, Mn, Ti, Zn, Co, Cu and V , etc. It has an excellent material removal function and heavy metal removal effect, and shows the effect of improving water quality.

상기 제1건조단계(S103)는 상기 원료혼합단계(S101)를 통해 제조된 혼합물을 건조하는 단계로, 상기 원료혼합단계(S101)를 통해 제조된 혼합물을 35 내지 55℃의 온도에서 15 내지 25시간 동안 건조하는 과정으로 이루어진다.The first drying step (S103) is a step of drying the mixture prepared through the raw material mixing step (S101), the mixture prepared through the raw material mixing step (S101) at a temperature of 35 to 55 ℃ 15 to 25 It consists of a drying process over time.

상기의 온도로 건조된 혼합물을 수분의 함량이 낮아져 분쇄과정의 효율성이 향상된다.The moisture content of the mixture dried at the above temperature is lowered, thereby improving the efficiency of the grinding process.

상기 미생물발효단계(S105)는 상기 제1건조단계(S103)를 통해 건조된 혼합물을 분쇄한 후에 유용미생물 혼합액을 혼합하고 발효하는 단계로, 상기 제1건조단계(S103)를 통해 건조된 혼합물을 80 내지 120메시의 입자크기로 분쇄한 후에, 분쇄된 혼합물 100 중량부에 유용미생물 혼합액 0.005 내지 0.01 중량부를 혼합하고 35 내지 55℃의 온도에서 10 내지 15시간 동안 발효하는 과정으로 이루어진다.The microbial fermentation step (S105) is a step of mixing and fermenting the mixture of useful microorganisms after pulverizing the mixture dried through the first drying step (S103), the dried mixture through the first drying step (S103) After grinding to a particle size of 80 to 120 mesh, 0.005 to 0.01 parts by weight of a mixture of useful microorganisms is mixed with 100 parts by weight of the pulverized mixture and fermented at a temperature of 35 to 55 ° C. for 10 to 15 hours.

이때, 상기 유용미생물 혼합액은 클로렐라, 유산균, 효모균, 고초균 및 황국균으로 이루어지며, 상기 유용미생물 혼합액은 클로렐라, 유산균, 효모균, 고초균 및 황국균이 1:1:1:1:1의 중량부로 혼합되어 이루어지는 것이 바람직한데, 상기의 성분으로 이루어지는 유용미생물 혼합물으로 발효되는 과정을 거치면, 유용미생물이 다량 증식되어 우수한 수질개선 효과를 나타낼 뿐만 아니라 보존성이 향상된 사료 조성물을 제공할 수 있다.At this time, the useful microorganism mixture is composed of chlorella, lactic acid bacteria, yeast, Bacillus subtilis, and Hwanggukgi, and the useful microorganism mixture is obtained by mixing chlorella, lactic acid bacteria, yeast, Bacillus subtilis, and Hwanggukgi in a weight part of 1: 1: 1: 1: 1 Preferably, when the fermentation process is performed with a useful microbial mixture composed of the above components, a large amount of useful microorganisms are proliferated to provide a feed composition with improved water quality as well as excellent water quality improvement effect.

이때, 상기 제1건조단계(S103)를 통해 건조된 혼합물을 80 내지 120 메시의 입자크기로 분쇄하는 이유는, 상기 제1건조단계(S103)를 통해 건조된 혼합물과 상기 유용미생물의 혼합도를 향상시켜 발효과정의 효율성을 향상기키기 위함인데, 상기 건조된 혼합물의 입자크기가 120 메시를 초과하게 되면 입자크기가 지나치게 작아 쉽게 비산되고 입자간의 뭉침현상이 발생하여 발효과정의 효율성이 저하되며, 상기 건조된 혼합물의 입자크기가 80 메시 미만이면 입자크기가 지나치게 커서 상기 유용미생물 혼합액과 접촉하는 면적이 지나치게 줄어들기 때문에 발효과정의 효율성이 저하될 수 있다.At this time, the reason for grinding the mixture dried through the first drying step (S103) to a particle size of 80 to 120 mesh is to determine the mixing degree of the mixture dried through the first drying step (S103) and the useful microorganisms. This is to improve the efficiency of the fermentation process by improving the efficiency of the fermentation process. When the particle size of the dried mixture exceeds 120 mesh, the particle size is too small and is easily scattered and agglomeration occurs between the particles, thereby reducing the efficiency of the fermentation process, If the particle size of the dried mixture is less than 80 mesh, the efficiency of the fermentation process may decrease because the particle size is too large and the contact area with the useful microbial mixture solution is excessively reduced.

또한, 상기 미생물발효단계(S105)에서 유용미생물 혼합액의 함량이 0.005 중량부 미만이면 발효과정이 지나치게 더기게 진행되며, 상기 유용미생물 혼합액의 함량이 0.01 중량부를 초과하게 되면 발효과정의 효율성은 크게 향상되지 않으면서 제조비용을 지나치게 증가시키기 때문에 바람직하지 못하다.In addition, in the microbial fermentation step (S105), when the content of the mixed solution of useful microorganisms is less than 0.005 parts by weight, the fermentation process proceeds too slowly, and when the content of the mixed solution of useful microorganisms exceeds 0.01 parts by weight, the efficiency of the fermentation process is greatly improved. It is undesirable because it excessively increases the manufacturing cost without being

상기 제2건조단계(S107)는 상기 미생물발효단계(S105)를 통해 발효된 혼합물을 건조하는 단계로, 상기 미생물발효단계(S105)를 통해 발효된 혼합물을 35 내지 55℃의 온도로 건조하여 수분의 함량이 35 내지 45%를 나타내도록 하는 과정으로 이루어진다.The second drying step (S107) is a step of drying the mixture fermented through the microbial fermentation step (S105), and drying the mixture fermented through the microbial fermentation step (S105) at a temperature of 35 to 55 ° C. It consists of a process to show the content of 35 to 45%.

상기 제2건조단계(S107)를 통해 건조된 혼합물의 수분 함량이 45%를 초과하게 되면 지나치게 많은 수분으로 인해 쉽게 변질되며 성형성이 저하되고, 수분 함량이 35% 미만이면 수분의 함량이 지나치게 낮아 성형 후에 성형물의 수분함량이 지나치게 낮아져 외력에 의해 쉽게 부서지며 가루가 발생하기 때문에 바람직하지 못하다.When the moisture content of the mixture dried through the second drying step (S107) exceeds 45%, excessive moisture easily deteriorates and moldability deteriorates, and when the moisture content is less than 35%, the moisture content is too low. This is undesirable because the water content of the molded article is too low after molding, which is easily broken by an external force and powder is generated.

상기 성형단계(S109)는 상기 제2건조단계(S107)를 통해 건조된 혼합물을 분쇄한 후에 성형하는 단계로, 상기 제2건조단계(S107)를 통해 건조된 혼합물을 분쇄한 후에 스크루의 회전속도가 200 내지 300rpm인 압출기에 투입하고 40 내지 50℃의 온도로 압출하는 과정으로 이루어지는데, 상기의 과정을 통해 제조되는 사료 조성물은 일정한 형태로 성형될 뿐만아니라 우수한 형태안정성을 나타낸다.The forming step (S109) is a step of shaping after pulverizing the mixture dried through the second drying step (S107), and the rotational speed of the screw after pulverizing the dried mixture through the second drying step (S107). Is put into an extruder at 200 to 300 rpm and extruded at a temperature of 40 to 50 ° C., and the feed composition prepared through the above process is molded into a constant shape and exhibits excellent form stability.

이때, 상기 압출기 스크루의 회전속도가 200rpm 미만이면 성형물의 밀도가 지나치게 낮아 제조되는 사료 조성물의 형태안정성이 저하되며, 상기 압출기 스크루의 회선속도가 300rpm을 초과하게 되면 성형물의 밀도가 지나치게 높아 사료 조성물이 딱딱해지기 때문에 기호도가 저하될 수 있다.At this time, when the rotational speed of the extruder screw is less than 200 rpm, the density of the molding is too low, and the shape stability of the feed composition is reduced. Because it becomes hard, the preference may be lowered.

또한, 상기 압출기의 온도가 40℃ 미만이면 상기의 효과가 미미하며, 상기 압출기의 온도가 50℃를 초과하게 되면 성형물에 함유된 유용미생물의 사멸하여 수질개선효과가 저하될 수 있다.In addition, if the temperature of the extruder is less than 40 ° C., the above effect is insignificant, and if the temperature of the extruder exceeds 50 ° C., useful microorganisms contained in the molded article may be killed and the effect of improving water quality may be reduced.

이때, 상기 압출기의 Die 직경은 3.5 내지 4.5mm를 나타내는 것이 바람직하다.At this time, the diameter of the die of the extruder is preferably 3.5 to 4.5 mm.

상기 어유코팅단계(S111)는 상기 성형단계(S109)를 통해 제조된 성형물의 표면에 어유를 코팅하는 단계로, 상기 성형단계(S109)를 통해 제조된 성형물 100 중량부 대비 어유 1 내지 3 중량부를 분무하여 코팅하는 과정으로 이루어지는데, 상기의 어유코팅단계(S111)를 통해 성형물의 표면에 기름막이 형성되어 산소와의 접촉이 차단되기 때문에, 보존성이 더욱 향상될 뿐만 아니라, 화학보존제의 사용으로 인한 유해성의 염려가 없고, 사료 조성물의 기호도가 저하되는 문제점을 해소할 수 있다.The fish oil coating step (S111) is a step of coating fish oil on the surface of the molding manufactured through the molding step (S109), and 1 to 3 parts by weight of fish oil relative to 100 parts by weight of the molding manufactured through the molding step (S109). It consists of a process of spraying and coating. Since an oil film is formed on the surface of the molding through the above fish oil coating step (S111) to block contact with oxygen, not only the preservation property is further improved, but also the use of a chemical preservative There is no concern about harmfulness, and it is possible to solve the problem that the preference of the feed composition is lowered.

이때, 어유는 정어리, 고등어 및 대구로부터 생성된 어유를 사용하는 것이 바람직한데, 상기 어유의 코팅량이 1 중량부 미만이면 상기의 효과가 미미하며, 상기 어유의 코팅량이 3 중량부를 초과하게 되면 상기의 효과는 크게 향상되지 않으면서 제조비용을 지나치게 증가시킬 뿐만 아니라, 어유의 성분의 지나친 사용으로 인해 수질오염이 발생할 수 있기 때문에 바람직하지 못하다.At this time, it is preferable to use fish oil produced from sardines, mackerel and cod as the fish oil. If the coating amount of the fish oil is less than 1 part by weight, the above effect is insignificant, and if the coating amount of the fish oil exceeds 3 parts by weight, the above It is undesirable because the production cost is excessively increased while the effect is not greatly improved, and water pollution may occur due to excessive use of fish oil components.

이하에서는, 본 발명에 따른 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법 및 그 제조방법으로 제조된 수질개선용 사료 조성물의 물성을 실시예를 들어 설명하기로 한다.Hereinafter, a method for producing a feed composition for improving water quality using useful microorganisms according to the present invention and physical properties of the feed composition for improving water quality produced by the manufacturing method will be described with examples.

<제조예 1> 어분의 제조<Production Example 1> Production of fishmeal

정어리, 고등어 및 대구가 1:1:1의 중량부로 혼합하여 어분을 제조하였다.Fishmeal was prepared by mixing sardines, mackerel, and cod in a weight part of 1:1:1.

<제조예 2> 미량영양소의 제조<Preparation Example 2> Preparation of micronutrients

비타민 혼합물(비타민 C와 비타민 A가 1:1의 중량부로 혼합), 새우분말, 다시마 및 스피룰리나를 1:1:1:1의 중량부로 한합하여 미량영양소를 제조하였다.Micronutrients were prepared by combining a vitamin mixture (vitamin C and vitamin A in a weight ratio of 1:1), shrimp powder, kelp, and spirulina in a weight ratio of 1:1:1:1.

<제조예 3> 유용미생물 혼합액의 제조<Preparation Example 3> Preparation of a mixture of useful microorganisms

클로렐라, 유산균, 효모균, 고초균 및 황국균을 1:1:1:1:1의 중량부로 혼합하여 유용미생물 혼합액을 제조하였다.A useful microorganism mixture was prepared by mixing chlorella, lactic acid bacteria, yeast bacteria, Bacillus subtilis, and Aspergillus lactobacillus in a weight part of 1: 1: 1: 1: 1.

<실시예 1><Example 1>

상기 제조예 1을 통해 제조된 어분 100 중량부, 소맥분 75 중량부, 전분 25 중량부, 탈피대두박 50 중량부, 깻묵 25 중량부, 옥수수 25 중량부 및 상기 제조예 2를 통해 제조된 미량영양소 75 중량부를 혼합하여 교반기가 구비된 혼합장치에 투입하고 150rpm의 속도로 10분 동안 교반하여 혼합물을 제조하고, 제조된 혼합물을 45℃의 온도에서 20시간 동안 건조하고, 건조된 혼합물을 분쇄장치에 투입하고 100메시의 입자크기로 분쇄한 후에, 분쇄된 혼합물 100 중량부에 상기 제조예 3을 통해 제조된 유용미생물 혼합액 0.008 중량부를 혼합하고 45℃의 온도에서 12시간 동안 발효하고, 발효된 혼합물을 45℃의 온도에서 20시간 동안 건조하고, 건조된 혼합물을 압출기에 투입하고 스크루의 회전속도가 250rpm인 조건에서 40℃의 온도로 4밀리미터의 다이를 통해 압출하고 절단 및 냉각하여 펠릿형태로 제조한 후에, 펠릿형태로 제조된 혼합물 100 중량부의 표면에 어유(정어리) 2 중량부를 분무하여 유용미생물을 활용한 수질개선용 사료 조성물을 제조하였다.100 parts by weight of fish meal, 75 parts by weight of wheat flour, 25 parts by weight of starch, 50 parts by weight of peeled soybean meal, 25 parts by weight of seed meal, 25 parts by weight of corn and 75 parts by weight of micronutrients prepared in Preparation Example 2 After mixing parts by weight, put them into a mixing device equipped with a stirrer, stir at a speed of 150 rpm for 10 minutes to prepare a mixture, dry the prepared mixture at a temperature of 45 ° C. for 20 hours, and put the dried mixture into a grinding device. After grinding to a particle size of 100 mesh, 0.008 parts by weight of the useful microorganism mixture prepared in Preparation Example 3 was mixed with 100 parts by weight of the pulverized mixture, fermented at a temperature of 45 ° C. for 12 hours, and the fermented mixture was 45 After drying at a temperature of ° C. for 20 hours, the dried mixture was put into an extruder, extruded through a 4 mm die at a temperature of 40 ° C. under the condition that the rotational speed of the screw was 250 rpm, cut and cooled to form pellets A feed composition for improving water quality using useful microorganisms was prepared by spraying 2 parts by weight of fish oil (sardine) on the surface of 100 parts by weight of the mixture prepared in the form of pellets.

<실시예 2><Example 2>

상기 실시예 1과 동일하게 진행하되, Proceed in the same manner as in Example 1,

어분 100 중량부 대비 첨가제(농축대두단백 및 칠면초가 1:1의 중량부로 혼합) 20 중량부를 더 혼합하여 유용미생물을 활용한 수질개선용 사료 조성물을 제조하였다.A feed composition for water quality improvement using useful microorganisms was prepared by further mixing 20 parts by weight of additives (concentrated soybean protein and chilmyeoncho mixed in a 1:1 weight part) with respect to 100 parts by weight of fish meal.

<실시예 3><Example 3>

상기 실시예 1과 동일하게 진행하되,Proceed in the same manner as in Example 1,

어분 100 중량부 대비 광물혼합물(맥반석, 펄라이트 및 일라이트가 1:1:1의 중량부로 혼합) 12 중량부를 더 혼합하여 유용미생물을 활용한 수질개선용 사료 조성물을 제조하였다.A feed composition for water quality improvement using useful microorganisms was prepared by further mixing 12 parts by weight of the mineral mixture (elvan, pearlite and illite mixed in a 1: 1: 1 weight part) with respect to 100 parts by weight of fish meal.

<비교예 1><Comparative Example 1>

어분 100 중량부, 소맥분 75 중량부, 전분 25 중량부, 탈피대두박 50 중량부, 깻묵 25 중량부, 옥수수 25 중량부 및 상기 제조예 2를 통해 제조된 미량영양소 75 중량부를 혼합하여 교반기가 구비된 혼합장치에 투입하고 150rpm의 속도로 10분 동안 교반하여 혼합물을 제조하고, 제조된 혼합물을 45℃의 온도에서 20시간 동안 건조하고, 건조된 혼합물을 압출기에 투입하고 스크루의 회전속도가 250rpm인 조건에서 40℃의 온도로 직경이 4 밀리미터인 다이를 통해 압출하고 절단 및 냉각하여 펠릿형태의 사료 조성물을 제조하였다.100 parts by weight of fish meal, 75 parts by weight of wheat flour, 25 parts by weight of starch, 50 parts by weight of peeled soybean meal, 25 parts by weight of seed meal, 25 parts by weight of corn, and 75 parts by weight of the micronutrients prepared in Preparation Example 2 were mixed to obtain a stirrer. The mixture was put into a mixing device and stirred at a speed of 150 rpm for 10 minutes to prepare a mixture, the prepared mixture was dried at a temperature of 45 ° C. for 20 hours, and the dried mixture was put into an extruder and the rotation speed of the screw was 250 rpm. At a temperature of 40 ° C., extruded through a die having a diameter of 4 mm, cut and cooled to prepare a feed composition in the form of pellets.

<비교예 2><Comparative Example 2>

상기 실시예 1과 동일하게 진행하되, 어유 10 중량부를 분무하여 사료 조성물을 제조하였다.Proceed in the same manner as in Example 1, but by spraying 10 parts by weight of fish oil to prepare a feed composition.

상기 실시예 1을 통해 제조된 사료 조성물의 영양성분을 분석하여 아래 표 1에 나타내었다.Nutrient components of the feed composition prepared in Example 1 were analyzed and shown in Table 1 below.

{단, 사료 조성물의 영양성분 분석은 농업실용화재단에 의뢰하여 분석하였다.}{However, analysis of the nutritional components of the feed composition was analyzed by requesting the Agricultural Commercialization Foundation.}

<표 1><Table 1>

Figure 112022105696364-pat00001
Figure 112022105696364-pat00001

상기 표 1에 나타낸 것처럼, 본 발명의 실시예 1을 통해 제조된 수질개선용 사료 조성물은 조단백질의 함량이 22% 이상을 나타내어 관상어를 포함한 어류등의 영양 섭취에 충분한 효과를 나타낼 수 있으며, 조지방, 칼슘 조섬유 조회분 및 인도 적합한 함량을 나타내는 것을 알 수 있다.As shown in Table 1, the feed composition for improving water quality prepared in Example 1 of the present invention has a crude protein content of 22% or more, so that it can show a sufficient effect on nutrient intake of fish, including ornamental fish, and crude fat, It can be seen that calcium crude ash and India exhibit suitable contents.

또한, 상기 실시예 1 내지 3 및 비교예 1 내지 2를 통해 제조된 사료 조성물의 수질개선 효능을 측정하여 아래 표 2에 나타내었다.In addition, the water quality improvement efficacy of the feed compositions prepared through Examples 1 to 3 and Comparative Examples 1 to 2 was measured and shown in Table 2 below.

{단, 수질개선 효능은 실시예 1 내지 3 및 비교예 1 내지 2를 통해 제조된 사료 조성물을 급여한 후 180일 동안 물을 환수하지 않은 상태에서 총질소와 총인의 함량을 측정하는 방법을 이용하였으며, 총질소와 총인의 함량은 흡광광도법(UV)을 이용하여 측정하였다.}{However, the water quality improvement effect uses a method of measuring total nitrogen and total phosphorus contents without water exchange for 180 days after feeding the feed compositions prepared through Examples 1 to 3 and Comparative Examples 1 to 2 The contents of total nitrogen and total phosphorus were measured using absorbance photometry (UV).}

<표 2><Table 2>

Figure 112022105696364-pat00002
Figure 112022105696364-pat00002

상기 표 2에 나타낸 것처럼, 본 발명의 실시예 1 내지 3을 통해 제조되는 사료 조성물은 비교예 1 내지 2를 통해 제조된 사료 조성물에 비해 수질개선 효과가 우수한 것을 알 수 있다.As shown in Table 2, it can be seen that the feed composition prepared in Examples 1 to 3 of the present invention has an excellent water quality improvement effect compared to the feed composition prepared in Comparative Examples 1 to 2.

따라서, 본 발명에 따른 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법은 영양성분이 풍부하게 함유되어 있을 뿐만 아니라, 유용미생물로 발효되어 우수한 수질개선 효과와 보존성을 나타내는 사료 조성물을 제공한다.Therefore, the method for producing a feed composition for improving water quality using useful microorganisms according to the present invention provides a feed composition that is fermented with useful microorganisms and exhibits excellent water quality improvement effect and preservation as well as rich in nutrients.

S101 ; 원료혼합단계
S103 ; 제1건조단계
S105 ; 미생물발효단계
S107 ; 제2건조단계
S109 ; 성형단계
S111 ; 어유코팅단계
S101; Raw material mixing step
S103; 1st drying step
S105; Microbial fermentation step
S107; 2nd drying step
S109; molding step
S111; Fish oil coating step

Claims (10)

어분, 소맥분, 전분, 탈피대두박, 깻묵, 옥수수 및 미량영양소를 혼합하는 원료혼합단계;
상기 원료혼합단계를 통해 제조된 혼합물을 건조하는 제1건조단계;
상기 제1건조단계를 통해 건조된 혼합물을 분쇄한 후에 유용미생물 혼합액을 혼합하고 발효하는 미생물발효단계;
상기 미생물발효단계를 통해 발효된 혼합물을 건조하는 제2건조단계;
상기 제2건조단계를 통해 건조된 혼합물을 분쇄한 후에 성형하는 성형단계; 및
상기 성형단계를 통해 제조된 성형물의 표면에 어유를 코팅하는 어유코팅단계;로 이루어지고,
상기 유용미생물 혼합액은 클로렐라, 유산균, 효모균, 고초균 및 황국균으로 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법.
A raw material mixing step of mixing fish meal, wheat flour, starch, hulled soybean meal, seed meal, corn, and micronutrients;
A first drying step of drying the mixture prepared through the raw material mixing step;
A microbial fermentation step of mixing and fermenting a mixture of useful microorganisms after pulverizing the dried mixture through the first drying step;
A second drying step of drying the mixture fermented through the microbial fermentation step;
A molding step of pulverizing and then shaping the dried mixture through the second drying step; and
A fish oil coating step of coating fish oil on the surface of the molded article produced through the molding step;
The method of producing a feed composition for improving water quality using useful microorganisms, characterized in that the useful microorganism mixture is composed of chlorella, lactic acid bacteria, yeast, Bacillus subtilis and Hwanggukgyun.
청구항 1에 있어서,
상기 원료혼합단계는 어분 100 중량부, 소맥분 70 내지 80 중량부, 전분 20 내지 30 중량부, 탈피대두박 45 내지 55 중량부, 깻묵 20 내지 30 중량부, 옥수수 20 내지 30 중량부 및 미량영양소 70 내지 80 중량부로 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법.
The method of claim 1,
In the raw material mixing step, 100 parts by weight of fish meal, 70 to 80 parts by weight of wheat flour, 20 to 30 parts by weight of starch, 45 to 55 parts by weight of peeled soybean meal, 20 to 30 parts by weight of seed meal, 20 to 30 parts by weight of corn, and 70 to 30 parts by weight of micronutrients Method for producing a feed composition for improving water quality using useful microorganisms, characterized in that consisting of 80 parts by weight.
청구항 2에 있어서,
상기 원료혼합단계에서는 상기 어분 100 중량부 대비 첨가제 15 내지 25 중량부가 더 함유되며,
상기 첨가제는 농축대두단백 및 칠면초로 이루어진 그룹에서 선택된 하나 이상으로 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법.
The method of claim 2,
In the raw material mixing step, 15 to 25 parts by weight of additives are further contained relative to 100 parts by weight of the fish meal,
The additive is a method for producing a feed composition for improving water quality using useful microorganisms, characterized in that consisting of at least one selected from the group consisting of concentrated soybean protein and chilmyeoncho.
청구항 1 내지 3 중 어느 한 항에 있어서,
상기 어분은 정어리, 고등어 및 대구가 1:1:1의 중량부로 혼합되어 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법.
According to any one of claims 1 to 3,
The fishmeal is a method for producing a feed composition for improving water quality using useful microorganisms, characterized in that sardines, mackerel and cod are mixed in a weight part of 1: 1: 1.
청구항 1 또는 2에 있어서,
상기 미량영양소는 비타민 혼합물, 새우분말, 다시마 및 스피룰리나로 이루어진 그룹에서 선택된 하나 이상으로 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법.
According to claim 1 or 2,
The micronutrient is a method for producing a feed composition for improving water quality using useful microorganisms, characterized in that consisting of one or more selected from the group consisting of vitamin mixture, shrimp powder, kelp and spirulina.
청구항 1에 있어서,
상기 미생물발효단계는 상기 제1건조단계를 통해 건조된 혼합물을 분쇄한 후에, 분쇄된 혼합물 100 중량부 대비 유용미생물 혼합액 0.005 내지 0.01 중량부를 혼합하고 35 내지 55℃의 온도에서 10 내지 15시간 동안 발효하여 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법.
The method of claim 1,
In the microbial fermentation step, after pulverizing the mixture dried through the first drying step, 0.005 to 0.01 parts by weight of a mixed solution of useful microorganisms is mixed with 100 parts by weight of the pulverized mixture and fermented at a temperature of 35 to 55 ° C. for 10 to 15 hours. Method for producing a feed composition for improving water quality using useful microorganisms, characterized in that made by.
삭제delete 청구항 1에 있어서,
상기 유용미생물 혼합액은 클로렐라, 유산균, 효모균, 고초균 및 황국균이 1:1:1:1:1의 중량부로 혼합되어 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법.
The method of claim 1,
The useful microbial mixture is a method for producing a feed composition for improving water quality using useful microorganisms, characterized in that chlorella, lactic acid bacteria, yeast, Bacillus subtilis and Hwanggukgyun are mixed in a weight part of 1: 1: 1: 1: 1.
청구항 1에 있어서,
상기 성형단계는 상기 제2건조단계를 통해 건조된 혼합물을 분쇄한 후에 스크루의 회전속도가 200 내지 300rpm인 압출기에 투입하고 40 내지 50℃의 온도로 압출하여 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법.
The method of claim 1,
The molding step utilizes useful microorganisms, characterized in that the mixture dried through the second drying step is pulverized and then put into an extruder having a screw rotational speed of 200 to 300 rpm and extruded at a temperature of 40 to 50 ° C. Method for producing a feed composition for improving water quality.
청구항 1에 있어서,
상기 원료혼합단계에서는 상기 어분 100 중량부 대비 광물혼합물 5 내지 20 중량부가 더 함유되며,
상기 광물혼합물은 맥반석, 펄라이트 및 일라이트가 1:1:1의 중량부로 혼합되어 이루어지는 것을 특징으로 하는 유용미생물을 활용한 수질개선용 사료 조성물의 제조방법.
The method of claim 1,
In the raw material mixing step, 5 to 20 parts by weight of the mineral mixture is further contained relative to 100 parts by weight of the fish meal,
The mineral mixture is a method for producing a feed composition for improving water quality using useful microorganisms, characterized in that elvan, perlite and illite are mixed in a weight part of 1: 1: 1.
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