CN111418442B - Method for preparing needle mushroom sticks from angelica sinensis dregs and beef cattle feed - Google Patents

Method for preparing needle mushroom sticks from angelica sinensis dregs and beef cattle feed Download PDF

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CN111418442B
CN111418442B CN202010187808.0A CN202010187808A CN111418442B CN 111418442 B CN111418442 B CN 111418442B CN 202010187808 A CN202010187808 A CN 202010187808A CN 111418442 B CN111418442 B CN 111418442B
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dregs
parts
needle mushroom
flammulina velutipes
angelica
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CN111418442A (en
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徐兵
周惠杰
周宇杰
孙飞
卢嘉敏
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Anhui Jisu Biotechnology Co ltd
Chengdu Yishenrui Technology Co ltd
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Changshu Institute of Technology
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Chemical & Material Sciences (AREA)
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  • Inorganic Chemistry (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a method for preparing needle mushroom sticks from angelica sinensis dregs and beef cattle feed, and the preparation method comprises the following steps: (1) pretreatment of medicine dregs: drying the angelica dregs; (2) activating strains: inoculating the needle mushroom slant, and placing into a liquid culture medium for shake flask culture; (3) bioconversion of flammulina velutipes: putting liquid strains in the liquid culture medium into the angelica sinensis dregs, and obtaining needle mushroom sticks after the bags are full; the beef cattle feed comprises the following components in parts by weight: 220 portions of rapeseed meal, 160 portions of bran, 10 to 14 portions of shell ash, 0.4 to 0.8 portion of salt and 780 portions of flammulina velutipes fungus stick; the feed can effectively regulate the digestive ability and the growth and development speed of the cattle, the growth and development speed of the fed cattle is 15.7-30.5% higher than that of the cattle fed by the conventional feed on average, and the disease rate of the fed cattle is reduced by 14-18%.

Description

Method for preparing needle mushroom sticks from angelica sinensis dregs and beef cattle feed
Technical Field
The invention relates to a method for preparing needle mushroom sticks from Chinese angelica dregs. The invention also relates to beef cattle feed containing the flammulina velutipes fungus sticks.
Background
In recent years, the feed industry in China is rapidly developed (the total value of the feed industry in China in 2018 reaches 8870 billion yuan, the total feed yield reaches 2.28 billion tons, the feed industry is the first to live in the world for 8 continuous years and accounts for 1/4 of the total global yield), although the total amount is very large, a plurality of problems also occur:
1. the feed safety problem in China is not optimistic, the feed sanitation standard is still imperfect, and the requirements on feed safety management can not be met, for example: 1) the allowable content of a plurality of toxic and harmful additives in the feed has not been established, and the established sanitation standard is limited to the feed of the pigs and the poultry; 2) investigation shows that part of enterprises use drugs or chemicals which are prohibited by certain countries for a long time in feed for commercial purposes, resulting in increased drug and chemical residues; 3) in addition, the feed raw materials of some enterprises are unreasonable in configuration, and heavy metals and harmful substances are seriously remained.
2. The current situation of resources in China: 1) the total amount of natural resources is large, the variety is large, but the occupied amount of human resources is small, and the development difficulty is large; 2) the resource development and utilization in China are unreasonable and scientific, and the waste and the loss are serious. Therefore, combining the above two points, the resource shortage of high quality feed will be a long and serious problem.
Generally, cattle have four stomachs, so rumination can occur. Cattle eat plants, and the fibers of the plants are relatively indigestible. The plant is fed by cattle, chewed roughly and enters rumen, and then can return to mouth for continuous chewing when having a rest. This is rumination. Rumination has four stages, food from the stomach back to the very mouth, chewing, mixing with saliva, and swallowing again. The digestion of food in the rumen greatly affects the health of cattle.
The beef contains rich protein, the amino acid composition is closer to the requirements of human bodies than pork, the disease resistance of the organisms can be improved, and the beef is particularly suitable for supplementing blood loss, repairing tissues and the like for people after growth, operation and diseases. Eating beef in winter has the function of warming stomach, and is a good tonic product in winter. The traditional Chinese medicine considers that: the beef has the functions of strengthening the middle-jiao and replenishing qi, nourishing spleen and stomach, strengthening bones and muscles, eliminating phlegm and calming endogenous wind, quenching thirst and stopping salivation. Is suitable for people with sinking of middle qi, short breath, weak constitution, soreness of bones and muscles, anemia, and blurred vision. Therefore, beef is more and more favored by people, and the market demand is increased year by year. In addition, from the development trend, the beef product demand will be larger and larger, and the beef production market prospect is wide.
According to the medical big data report of the research institute of the Chinese trade industry, the proportion of the traditional Chinese medicine is getting bigger and bigger (3462 medical hospitals in 2016, the proportion is 11.9%, and the proportion is increased by 5.6%), and the common treatment modes of the traditional Chinese medicine residues are stacking, burning and burying, and the treatment modes not only need enterprises to bear the pollution discharge cost, but also cause certain pollution to water quality, air and soil. However, researches show that the angelica dregs contain nutrient components such as crude fat, crude protein, amino acid, trace elements and the like, and certain amount of bioactive components such as polysaccharide, flavone, saponin and the like are remained.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for preparing needle mushroom sticks from Chinese angelica dregs, and also provides a beef cattle feed containing the needle mushroom sticks.
In order to solve the technical problems, the method for preparing the needle mushroom sticks from the angelica sinensis dregs comprises the following steps:
(1) pretreatment of medicine dregs: drying the angelica sinensis dregs. Specifically, the Chinese angelica dregs are dried by a microwave drying technology, and then the crushed dregs are treated by a differential pressure puffing technology.
The Chinese angelica dregs are dried by a microwave drying technology, so that the putrefying bacteria pollution can be effectively prevented; the crushed medicine dregs are treated by a differential pressure puffing technology, so that gaps are generated in the dried medicine dregs, and hypha growth and medicinal components are favorably separated out. The traditional Chinese medicine dregs are generally wet materials, are extremely easy to rot and smelly, are subjected to microwave drying (6000W, single-layer tiling and half an hour of treatment) in the treatment process, are short in time, can effectively control the pollution of putrefying bacteria, but in the drying process, the organizational structure of the dregs is changed, becomes compact and has hardened surfaces, which are not beneficial to the taking of flammulina velutipes hyphae, then the dried dregs are subjected to differential pressure puffing operation, after the dregs enter a puffing cavity, the materials are extruded, rubbed, sheared and the like between screw thread sleeves, the internal pressure of the materials is continuously increased, the maximum pressure is 4MPa, and the temperature is continuously increased, for example, the maximum temperature can reach 140 ℃. The rapid rise of temperature and pressure in a short time (such as 3-7s) causes the change of the tissue structure of the dregs of decoction, and the destruction of coarse fibers. The high-temperature and high-pressure materials are discharged from the discharge port, the pressure of the materials is suddenly released instantly, partial flash evaporation is carried out on moisture, the cooled medicine residues are in a loose and porous structure, needle mushroom hypha can enter the medicine residues along gaps to grow and fully transform the medicine residues, and after the medicine residues are puffed by pressure difference, the residual medicinal components are more easily separated out due to the change of the tissue structure.
(2) Activating strains: inoculating the needle mushroom slant, and placing into a liquid culture medium for shake culture. The strain of the needle mushroom is needle mushroom of GIM5.185 (the GIM number of Guangdong province microbial strain preservation center is GIM5.185, and the strain name is needle mushroom strain).
(3) Bioconversion of flammulina velutipes: and putting liquid strains in the liquid culture medium into the angelica sinensis dregs, bagging and culturing, and obtaining the needle mushroom sticks after the bags are full. Specifically, the mass ratio of the liquid strains to the angelica sinensis dregs is 3:7, and the needle mushroom sticks are obtained after the bags are filled for 30 days.
The main component of the angelica sinensis dregs is cellulose and angelica sinensis polysaccharide, through the material mixing type inoculation with the mass ratio of the flammulina velutipes liquid strain to the angelica sinensis dregs being 3:7, hypha can grow over the compost in 3 days, the hypha can directly utilize the angelica sinensis polysaccharide to grow at the beginning, the inoculation amount with high proportion can inhibit the growth of mixed bacteria, the hypha can secrete cellulose degrading enzyme after utilizing the angelica sinensis polysaccharide, the cellulose is degraded and converted into nutrient substances required by the user, the crude protein content in the cultivation material is measured from the time when the hyphae begin to propagate in large quantity on the 7 th day until 30 days before fruiting, the hyphae are the most and the crude protein content is the highest, the culture material is added with a certain amount of shell ash to improve the content of crude protein and crude fiber of needle mushroom fruiting bodies, the contents of crude fat, crude polysaccharide and ash are not changed, the nutrition quality of the sporocarp is improved, and the content of crude protein in the cultivation material can reach 12.9%.
The beef cattle feed containing the needle mushroom sticks obtained by the method for preparing the needle mushroom sticks by using the angelica sinensis dregs comprises the following components in parts by weight: 180-220 parts of rapeseed meal, 120-160 parts of bran, 10-14 parts of shell ash, 0.4-0.8 part of salt and 450-780 parts of needle mushroom stick, and preferably comprises the following components in parts by weight: 180 parts of rapeseed meal, 120 parts of bran, 10 parts of shell ash, 0.4 part of salt and 450 parts of needle mushroom sticks. The shell ash is calcined shell powder, commonly called shell ash, and is prepared by selecting various shells such as oyster shell and the like, calcining the shells in a kiln at a high temperature of 800-1600 ℃, and grinding the shells into powder, wherein the main component is calcium oxide.
The invention has the beneficial effects that:
the beef cattle feed disclosed by the invention is reasonable in formula, relatively lower in cost, comprehensive in nutritional ingredients and free of additives, and when the beef cattle feed is taken by cattle, the appetite can be increased, the palatability of the feed is improved, the digestion and utilization rate is improved, the immunity of the cattle is enhanced, the biological activity of the cattle body is effectively enhanced, the in-vivo metabolism is promoted, the absorption and utilization of various nutrients in food are facilitated, the growth and development are greatly benefited, and the disease rate can be effectively reduced. The feed can effectively regulate the digestive ability and the growth and development speed of the cattle, the growth and development speed of the fed cattle is 15.7-30.5% higher than that of the cattle fed by the conventional feed on average, and the disease rate of the fed cattle is reduced by 14-18%.
Detailed Description
The strain of Flammulina velutipes (golden mushroom) is GIM5.185 (the number of GIM of Guangdong province microorganism culture Collection is GIM5.185, and the strain name is Flammulina velutipes).
Example 1
The beef cattle feed for converting the Chinese angelica dregs by utilizing the needle mushrooms comprises the following components in parts by weight: 220 parts of rapeseed meal, 160 parts of bran, 14 parts of shell ash, 0.8 part of salt and 650 parts of needle mushroom sticks.
The needle mushroom stick is obtained by the following method:
(1) pretreatment of medicine dregs: drying the residue with 6000W microwave for 30min, and puffing the dried residue under pressure difference: after the dregs of a decoction enter the puffing cavity, the materials are extruded, rubbed, sheared and the like between screw sleeves, the internal pressure of the materials is continuously increased to 4Mpa at most, and the temperature is continuously increased to 140 ℃ at most. The temperature and pressure rise rapidly within 3-7s, the texture of the dregs of decoction changes, and the crude fiber is destroyed. The high-temperature and high-pressure materials are discharged from the discharge hole, the pressure of the materials is suddenly released instantly, partial flash evaporation of water is carried out, and the medicine residues are in a loose and porous structure after cooling.
(2) Activating strains: inoculating the needle mushroom slant, and placing into a liquid culture medium for shake culture.
(3) Bioconversion of flammulina velutipes: putting liquid strains in a liquid culture medium into the medicine residues, wherein the mass ratio of the liquid strains to the medicine residues of the angelica is 3:7, bagging and culturing, and compounding the liquid strains with the components after the bag is filled for 30 days.
Example 2
Same as in example 1, with the difference: 180 parts of rapeseed meal, 120 parts of bran, 10 parts of shell ash, 0.4 part of salt and 450 parts of flammulina velutipes fungus sticks.
Example 3
As in example 1, with the following differences: 190 parts of rapeseed meal, 130 parts of bran, 11 parts of shell ash, 0.5 part of salt and 500 parts of needle mushroom sticks.
Example 4
As in example 1, with the following differences: 200 parts of rapeseed meal, 140 parts of bran, 12 parts of shell ash, 0.6 part of salt and 550 parts of needle mushroom sticks.
Example 5
Same as in example 1, with the difference: 210 parts of rapeseed meal, 150 parts of bran, 13 parts of shell ash, 0.7 part of salt and 600 parts of needle mushroom sticks.
Comparative example 1
The beef cattle feed comprises the following components in parts by weight: 220 parts of rapeseed meal, 160 parts of bran, 14 parts of shell ash, 0.8 part of salt and 130 parts of corn.
Comparative example 2
The beef cattle feed comprises the following components in parts by weight: 180 parts of rapeseed meal, 120 parts of bran, 10 parts of shell ash, 0.4 part of salt and 90 parts of corn.
Comparative example 3
The beef cattle feed comprises the following components in parts by weight: 220 parts of rapeseed meal, 160 parts of bran, 14 parts of shell ash, 0.8 part of salt and 650 parts of needle mushroom sticks.
The needle mushroom stick is obtained by the following method:
(1) pretreatment of medicine dregs: drying the residue with 6000W microwave for 30min, and puffing the dried residue under pressure difference: after the dregs of a decoction enter the puffing cavity, the materials are extruded, rubbed, sheared and the like between screw sleeves, the internal pressure of the materials is continuously increased to 4Mpa at most, and the temperature is continuously increased to 140 ℃ at most. The temperature and pressure rise rapidly within 3-7s, the texture of the dregs of decoction changes, and the crude fiber is destroyed. The high-temperature and high-pressure materials are discharged from the discharge hole, the pressure of the materials is suddenly released instantly, partial water is subjected to flash evaporation, and the cooled medicine residues are in a loose and porous structure.
(2) Activating strains: inoculating the needle mushroom slant, and placing into a liquid culture medium for shake culture.
(3) Bioconversion of flammulina velutipes: putting liquid strains in a liquid culture medium into the medicine residues, wherein the mass ratio of the liquid strains to the medicine residues of the angelica is 3:7, bagging and culturing, and compounding the liquid strains with the components after the bag is filled for 7 days.
Comparative example 4
The beef cattle feed comprises the following components in parts by weight: 220 parts of rapeseed meal, 160 parts of bran, 14 parts of shell ash, 0.8 part of salt and 650 parts of needle mushroom sticks.
The needle mushroom stick is obtained by the following method:
(1) pretreatment of medicine dregs: drying the residue with 6000W microwave for 30min, and puffing the dried residue under pressure difference: after the medicine dregs enter the puffing cavity, materials are extruded, rubbed, sheared and the like between screw rod thread sleeves, the internal pressure of the materials is continuously increased to be 4Mpa at most, the temperature is continuously increased to be 140 ℃ at most. The temperature and pressure rise rapidly within 3-7s, the texture of the dregs of decoction changes, and the crude fiber is destroyed. The high-temperature and high-pressure materials are discharged from the discharge hole, the pressure of the materials is suddenly released instantly, partial water is subjected to flash evaporation, and the cooled medicine residues are in a loose and porous structure.
(2) Activating strains: inoculating the needle mushroom slant, and placing into a liquid culture medium for shake culture.
(3) Bioconversion of flammulina velutipes: putting liquid strains in a liquid culture medium into the angelica sinensis dregs in a mass ratio of 3:7, bagging and culturing, and compounding the liquid strains and the dregs with the components after the bag is filled for 14 days.
Comparative example 5
The beef cattle feed comprises the following components in parts by weight: 220 parts of rapeseed meal, 160 parts of bran, 14 parts of shell ash, 0.8 part of salt and 650 parts of needle mushroom sticks.
The needle mushroom stick is obtained by the following method:
(1) pretreatment of medicine dregs: drying the residue with 6000W microwave for 30min, and puffing the dried residue under pressure difference: after the dregs of a decoction enter the puffing cavity, the materials are extruded, rubbed, sheared and the like between screw sleeves, the internal pressure of the materials is continuously increased to 4Mpa at most, and the temperature is continuously increased to 140 ℃ at most. The temperature and pressure rise rapidly within 3-7s, the texture of the dregs of decoction changes, and the crude fiber is destroyed. The high-temperature and high-pressure materials are discharged from the discharge hole, the pressure of the materials is suddenly released instantly, partial water is subjected to flash evaporation, and the cooled medicine residues are in a loose and porous structure.
(2) Activating strains: inoculating the needle mushroom slant, and placing into a liquid culture medium for shake culture.
(3) Bioconversion of flammulina velutipes: putting liquid strains in a liquid culture medium into the angelica sinensis dregs in a mass ratio of 3:7, bagging and culturing, and compounding the liquid strains and the dregs with the components after the bag is filled for 20 days.
Comparative example 6
Same as in example 1, with the difference: drying the radix Angelicae sinensis residue with hot air.
In the above examples and comparative examples, the nutrient components of the obtained feeds are shown in table 1.
TABLE 1 nutrient content comparison table
Crude protein (%) Calcium (%) Phosphorus (%)
Example 1 11.4 0.43 0.19
Example 2 13.9 0.46 0.18
Example 3 13.1 0.44 0.20
Example 4 12.5 0.38 0.11
Example 5 11.7 0.40 0.15
Comparative example 1 5.9 0.27 0.14
Comparative example 2 6.6 0.23 0.11
Comparative example 3 6.8 0.41 0.18
Comparative example 4 9.5 0.40 0.18
Comparative example 5 10.6 0.45 0.20
Comparative example 6 10.9 0.35 0.15
As can be seen from table 1 above:
1) the proportion of crude protein in examples 1-5 is significantly higher than in comparative examples 1, 2, indicating that the feed quality of the invention is far superior to conventional feeds. Therefore, the culture material is added with 10-14 parts of shell ash, so that the alkalinity of the culture material can be effectively improved, and the quality of hyphae can be effectively improved by taking 10 parts of the shell ash as a preferable additive amount.
2) The proportion of crude protein in examples 1-5 is also significantly higher than in comparative examples 3, 4 and 5, indicating that the formulation of the feed is optimal 30 days after filling.
3) Comparison of example 1 with comparative example 6 shows that: boiling and drying are beneficial to hypha growth and medicinal component precipitation.
In the above examples, the growth rate of hyphae is shown in Table 2.
TABLE 2 statistical table of hypha growth rate
Figure BDA0002414801500000071
Figure BDA0002414801500000081
Note that "+, + + + +" indicates that the hyphae grow sparsely, generally and robustly
As can be seen from Table 2, the addition of the shell ash in different proportions to different cultivation materials has a promoting effect on the growth of hyphae, wherein the hyphae treated by the formula with the shell ash content of 10 parts grow fastest and grow vigorously.
The cattle fed with the feed prepared according to the formulation of examples 1 to 5 and comparative examples 1 to 2 were fed for three months, and then the cattle fed with different feeds were observed for the difference in growth and disease (fed for 120 days).
TABLE 3 statistical Table of growth rates
Mean weight gain (1-3 months), units: g/day
Example 1 1522
Example 2 1765
Example 3 1695
Example 4 1623
Example 5 1589
Comparative example 1 1315
Comparative example 2 1298
1) As can be seen from table 3 above: the growth rate of the cattle fed the feed in examples 1-5 was 15.7-30.5% higher than that of the cattle fed the feed in comparative examples 1-2.
2) Meanwhile, statistics show that the disease rate of the cattle fed with the feed in the examples 1 to 5 is 14 to 18 percent lower than that of the cattle fed with the feed in the comparative examples 1 to 2.
The above embodiments do not limit the present invention in any way, and all technical solutions obtained by means of equivalent substitution or equivalent transformation fall within the protection scope of the present invention.

Claims (4)

1. The method for preparing the needle mushroom sticks from the angelica sinensis dregs is characterized by comprising the following steps:
(1) pretreatment of medicine dregs: drying the angelica dregs;
(2) activating strains: inoculating the needle mushroom slant, and putting the needle mushroom slant into a liquid culture medium for shake culture;
(3) bioconversion of flammulina velutipes: putting liquid strains in a liquid culture medium into the angelica sinensis dregs, bagging and culturing, and obtaining needle mushroom sticks after the bags are full;
in the step (1), drying the angelica dregs by using a microwave drying technology, and then treating the crushed dregs by using a differential pressure puffing technology;
firstly, drying the angelica sinensis dregs by using a microwave drying technology, wherein the time is short, the pollution of putrefying bacteria can be effectively controlled, but in the drying process, the organizational structure of the dregs is changed, the dregs become compact and hardened, the surface of the dregs is not beneficial to the eating of flammulina velutipes mycelium, then, the dried dregs are subjected to differential pressure puffing operation, the temperature and the pressure are rapidly increased in a short time, the organizational structure of the dregs is changed, crude fibers are destroyed, the dregs are in a loose and porous structure after cooling, the flammulina velutipes mycelium can enter the dregs along gaps to grow, the dregs are fully converted, and after the dregs are puffed by differential pressure, the remaining medicinal components are more easily separated out due to the change of the organizational structure;
in the step (3), the mass ratio of the liquid strains to the angelica dregs is 3: 7.
2. The method for preparing needle mushroom sticks from angelica sinensis dregs as claimed in claim 1, which is characterized in that: and (4) in the step (3), obtaining the flammulina velutipes fungus sticks after the bags are full for 30 days.
3. Beef cattle feed containing flammulina velutipes fungus sticks obtained by the method for preparing the flammulina velutipes fungus sticks from angelica sinensis dregs in claim 1 or 2 is characterized by comprising the following components in parts by weight: 220 portions of rapeseed meal, 160 portions of bran, 10 to 14 portions of shell ash, 0.4 to 0.8 portion of salt and 780 portions of flammulina velutipes fungus sticks.
4. The beef cattle feed according to claim 3, characterized by comprising the following components in parts by weight: 180 parts of rapeseed meal, 120 parts of bran, 10 parts of shell ash, 0.4 part of salt and 450 parts of needle mushroom sticks.
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