KR20150080405A - Fermentable stuff for sea cucumber and method for preparation thereof - Google Patents

Abstract

The present invention relates to powder-type or liquid-type microorganism fermented feed for sea cucumber and a method for manufacturing the same. The method includes: a first step of mixing and drying 10-20 wt% of Sargassum thunbergii, 1-20 wt% of lugworm, 5-20 wt% of seaweed, 5-20 wt% of kelp, 5-20 wt% of fusiformis, 5-20 wt% of gulfweed, 10-30 wt% of fish powder, 5-20 wt% of diatomite, and 10-30 wt% of a soybean cake; a second step of grinding the dried materials; a third step of adding and mixing 5-20 wt% of microorganisms with 80-95 wt% of the dried materials; a fourth step of mixing the mixture including the microorganisms with distilled water; and a fifth step of fermenting the mixture after placing the mixture in a fermentation apparatus.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a fermented feed of sea cucumber and a method for preparing the same,

The present invention relates to a fermented feed of sea cucumber microorganism and a method for producing the fermented feed,

Sea cucumber belongs to the sea eel of the echinoderm, which is called ginseng in the sea because it is similar to ginseng, and its popularity is increasing day by day as the best stamina food. The animals in the sea cucumber river are symmetrical in the body and are in the form of cucumbers and have no arms and thorns. Some of the species are less developed, some are degenerated and others are not. Muscles of the body wall are thick, and small fragments of calcareous matter are scattered inside.

The digestive tract is long and the anus is usually behind, and a pair of breathing water is in the total dung that leads to the anus. The length of the body is 2 to 150 cm. All live in the sea and are british. It is distributed in all seas and there are various places such as rock, clay, sand, seaweed. Eat suspensions or sediments.

The consumption of sea cucumber, which is the best stamina food, is increasing day by day due to the well-being trend of modern day. However, the quantity of natural sea cucumber is decreased due to environmental pollution, and the amount of catch is greatly decreased. Therefore, there is an urgent need to develop mass culture of sea cucumber and feed for sea cucumber cultivation.

Currently, there is a market for sea cucumber farming, but the immune system is low, resulting in high mortality rate and low rate of growth. Most of the Chinese feeds are pearl constituents supplementing minerals, including arsenic and heavy metals. It is true.

In addition, as a prior art document on current sea cucumber feedstuffs, in Korean Patent Laid-Open Publication No. 10-2005-0062763, " Method for preparing sea cucumber feedstuffs ", the main feed of sea cucumbers is marine algae such as seaweed, sea tangle, A method of dissolving in water to provide a celecoxine feed in the form of a juice has been disclosed in Korean Patent Laid-Open Publication No. 10-2006-0125455 entitled " , Seaweed, sea tangle, mackerel, and the like, granules containing fish meal, soybean meal, blood meal and the like, and then dissolving in water to provide celery feed in the form of juice is disclosed in Korean Patent No. 10-091548 In the "feed additive for sea cucumber", a cucumber feed additive having improved digestion and absorption rate and palatability, which contains a herbal supplement, a dandelion, a liquorice, licorice, Angelica gigas, and acacia var.

There is no technology to make the effect of the additional growth on the feed for the sea cucumber, and it is required to develop the feed for the sea cucumber which can exert an excellent effect on the sufficient immunity and the growth rate as well as the sufficient nutrient.

SUMMARY OF THE INVENTION It is an object of the present invention to provide a feed for sea cucumbers capable of exerting an excellent effect on not only nutrients but also excellent immunity and growth rate.

In order to attain the above object, the present invention relates to a method for producing a fish meat comprising 10-20% by weight of worms, 1-20% by weight of worms, 5-20% by weight of sea mustard, 5-20% by weight of kelp, 5-20% 10-30% by weight of fish meal, 5-20% by weight of diatomaceous earth, 10-30% by weight of soybean meal, and 5-20% by weight of microorganism.

The present invention also provides a fermented microorganism feed for sea cucumber, comprising 80-95% by weight of insect worms and 5-20% by weight of microorganisms.

The present invention also relates to a method for producing a microorganism, wherein the microorganism is Rhodobacter sphaeroides, Bacillus subtilus, Lactobacillus sp., Pediococcus sp., Sacharomyces cerevisiae, Nitrobactor sp. And Nitrosomonas sp. Wherein the microorganism fermented feed for sea cucumber is selected from the group consisting of:

The present invention also relates to a method for producing a fish meal comprising 10-20% by weight of worms, 1-20% by weight of worms, 5-20% by weight of sea mustard, 5-20% by weight of sea tangle, 5-20% 30 wt%, diatomaceous earth 5 - 20 wt%, and soybean meal 10 - 30 wt%; A second step of pulverizing the mixed material; Adding the microorganism to the pulverized material, further mixing 80 to 95% of the material and 5 to 20% by weight of the microorganism; A fourth step of mixing distilled water into the mixture of the microorganisms; And fermenting the mixture in a fermenter to produce a fermented microorganism feed for sea cucumber.

The present invention also provides a process for preparing a fermented microorganism feed for sea cucumber, characterized in that, in the first step, only the insect is dried or used after being washed in a natural state.

The present invention also provides a method for producing a fermented microorganism feed for sea cucumbers, comprising the sixth step of drying and crushing the fermented mixture further after the fifth step.

The present invention also relates to a method for producing a microorganism, wherein the microorganism is Rhodobacter sphaeroides, Bacillus subtilus, Lactobacillus sp., Pediococcus sp., Sacharomyces cerevisiae, Nitrobactor sp. And Nitrosomonas sp. Wherein the microbial fermented feed for sea cucumber is selected from the group consisting of:

Also, the present invention provides a method for preparing a fermented microorganism for sea cucumber, wherein the pulverization is performed at 100-120 mesh in the second step.

The fermented feed of sea cucumber microorganism according to the present invention is an advanced feed which is fermented into microorganisms by using natural materials, and the digestibility and absorption rate of the sea cucumber to amino acids of proteins are greatly increased, thereby increasing the growth rate by 20% or more than other feeds.

In addition, the fermented feed of the sea cucumber microorganism according to the present invention exerts an excellent effect on not only a sufficient nutrient but also excellent immunity and a growth rate, thereby bringing about effective growth of cultured sea cucumber in a short period of time.

In addition, the fermented feed of the sea cucumber microorganism according to the present invention is characterized by the fact that a protein source (60% or more of spirulina) such as vegetable such as seaweed, an enzyme hydrolyzed and extracted larvae, a suitable ratio of a carbohydrate source such as kelp and a diatomaceous earth As a microbial fermentation feed, the fermented feed enhances the survival rate and promotion of survival (25% or more) with a high digestion rate, and the fermentation microorganism has an effect of preventing sediment secretion under cultured bottom.

FIG. 1 is a schematic view illustrating a manufacturing process of a fermented microbial feed for sea cucumber according to an embodiment of the present invention.
FIG. 2 shows a liquid and powdery feed of a fermented microbial feed for sea cucumber according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings. First, it should be noted that, in the drawings, the same components or parts have the same reference numerals as much as possible. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted so as to avoid obscuring the subject matter of the present invention.

The terms " about "," substantially ", etc. used to the extent that they are used herein are intended to be taken to mean an approximation of, or approximation to, the numerical values of manufacturing and material tolerances inherent in the meanings mentioned, Accurate or absolute numbers are used to help prevent unauthorized exploitation by unauthorized intruders of the referenced disclosure.

The present invention relates to a fermented feed of sea cucumber microorganism and a method for producing the same, wherein 10-20% by weight of worms, 1-20% by weight of worms, 5-20% by weight of sea mustard, 5-20% by weight of kelp, A first step of mixing 5-20% by weight of mung bean, 10-30% by weight of fish meal, 5-20% by weight of diatomaceous earth and 10-30% by weight of soybean meal; A second step of pulverizing the mixed material; Adding the microorganism to the pulverized material, further mixing 80 to 95% of the material and 5 to 20% by weight of the microorganism; A fourth step of mixing distilled water into the mixture of the microorganisms; And fermenting the mixture in a fermenter.

In addition, it may further comprise a sixth step of drying and pulverizing the further fermented mixture after the fifth step.

In addition, in the first step, a liquid fermented feed can also be prepared by using microorganisms alone and mixing the microorganisms.

FIG. 1 is a schematic view illustrating a manufacturing process of a fermented microbial feed for sea cucumber according to an embodiment of the present invention. FIG. 2 shows a liquid and powdery feed of a fermented microbial feed for sea cucumber according to an embodiment of the present invention.

The present invention relates to a composition comprising 10-20% by weight of worms, 1-20% by weight of wormwood, 5-20% by weight of seaweed, 5-20% by weight of kelp, 5-20% by weight of topsoil, 5-20% By weight of diatomaceous earth, 5 to 20% by weight of diatomaceous earth and 10 to 30% by weight of soybean meal; A second step of pulverizing the mixed material; Adding the microorganism to the pulverized material, further mixing 80 to 95% of the material and 5 to 20% by weight of the microorganism; A fourth step of mixing distilled water into the mixture of the microorganisms; And a fifth step of fermenting the mixture into a fermenter.

In the first step, insects, lugworts, seaweeds, kelp, peat, mung bean, fish meal, diatomaceous earth and soybean meal can be mixed and optionally mixed and dried for powder. The mixing ratio of the above substances is 10-20% by weight, 10-20% by weight of worms, 5-20% by weight of sea mustard, 5-20% by weight of kelp, 5-20% by weight of topsoil, 5-20% 10-30% by weight of fish meal, 5-20% by weight of diatomaceous earth and 10-30% by weight of soybean meal.

In the first step, the diatomite is mixed and can act as a mineral supplement. When the diatomaceous earth is used, it can be safely used as food for sea cucumbers because there is no heavy metal content as compared with that used by using ordinary tidal flats.

In addition, as the soybean meal is mixed in the first step, the growth of the microorganisms added in the third step, which is a subsequent step, is activated, and the fermentation in the fifth step can be performed more easily.

In addition, the present invention may use only the insect worm in the first step. When the insect is fermented by mixing with microorganisms as food for sea cucumbers, the protein provided by the insect can be effectively fermented by the microorganisms to increase digestion and absorption rate.

In the case where the powdery feed is prepared in the first step, it can be selectively dried, and the drying can be naturally dried or dried at 30 to 40 ° C. In this case, it is preferable that the moisture content of the material is 15% or less.

In the second step, the mixed material can be pulverized, and the pulverized material is preferably pulverized to 100-120 mesh. Mixing with the microorganism can be properly mixed when pulverized in this manner.

In the third step, microorganisms can be added, and the microorganisms can be further mixed at a ratio of 80 to 95% of the dried material in step 1 and 5 to 20% by weight of the microorganism.

Specific examples of the microorganism include Rhodobacter sphaeroides, Bacillus subtilus, Lactobacillus sp., Pediococcus sp., Sacharomyces cerevisiae, Nitrobactor sp. And Nitrosomonas sp. May be formed to include at least one group.

When the fermentation is carried out at an appropriate temperature after adding the microorganism, the digestion absorption rate is greatly increased, and the growth rate of the sea cucumber is increased.

Distilled water can be mixed after the third step of adding and mixing microorganisms. By mixing the distilled water, the materials mixed with the microorganisms can be mixed well, and the fermentation can be performed at an appropriate moisture content in the fermentation in the fermentation.

The distilled water may be mixed with distilled water in a volume ratio of 1: 2 to 1:10 in relation to the material in which the microorganisms are mixed in three stages. The fermented feed of the present invention can be prepared for liquid or powder, and can be appropriately adjusted according to the mixing ratio of the distilled water. For example, distilled water may be slightly mixed when prepared into a liquid form, and may be mixed with distilled water when prepared into a powder form.

The fifth step is a step of fermenting a substance in which the microorganism and the distilled water are further mixed, and the substance in which the microorganism and the distilled water are mixed can be fermented in a fermenter.

The fermenter is not particularly limited and can be fermented while maintaining a temperature of about 30 to 40 캜. The fermentation time in the fermenter is preferably about 24 to 36 hours.

Referring to FIG. 2, the fermented material can be used as a direct feed. In this case, the fermented material may be made into a liquid feed or a powder feed.

In the sixth step, the powdery fermented feed is prepared by drying so that the water content is less than 10% while maintaining the drying temperature at 30 to 40 ° C in order to produce the powdery feed.

The prepared fermented microbial feed for sea cucumber can provide liquid or powdery feed, and the digestion rate is greatly increased, so that the growth rate is higher than other feeds.

Hereinafter, embodiments of the present invention will be described in detail.

Example  One

10 wt% of sea mustard, 10 wt% of sea tangle, 10 wt% of mung bean, 10 wt% of mackerel, 20 wt% of fish meal, and dried at about 30 캜 to have a moisture content of 10% (Step 1). The dried material is then pulverized to 100 mesh (second step). To add the microorganism to the milled material, 80 wt% of the dried material and 20 wt% of the microorganism are mixed. At this time, the added microorganisms are added at the same ratio of Bacillus subtilus and Lactobacilus sp. (Step 3)

Thereafter, distilled water is mixed with the distilled water to the microorganism-added mixed material at a ratio of 1: 5 (Step 4).

The material mixed with distilled water is put into a fermenter and fermented for 24 hours (Step 5). After the fermentation is completed in this manner, the powdered fermented feed can be completed.

Using this, we observed the growth of young sea cucumber as feed.

Example  2

The same procedure as in Example 1 was carried out. In the first step, only the worms were washed in a natural state. The subsequent steps 2, 3, and 5 are performed in the same manner as in Example 1, and in the fourth step, distilled water is mixed at a ratio of 1: 8 relative to the microbial additional mixed material to prepare a liquid feed .

Example  3

10 wt% of sea mustard, 10 wt% of sea tangle, 10 wt% of mung bean, 10 wt% of mackerel, 25 wt% of fish meal, and dried at about 30 캜 to have a moisture content of 10% (Step 1). The dried material is then pulverized to 120 mesh (second step). To add the microorganism to the milled material, 85 wt% of the dried material and 15 wt% of the microorganism are mixed. The microorganisms added at this time were Sacharomyces cerevisiae, Nitrobactor sp. And Nitrosomonas sp. Are added at the same rate (step 3).

Then, distilled water is mixed with distilled water in a mixed ratio of 1: 3 (Step 4).

The material mixed with distilled water is put into a fermenter and fermented for 36 hours (Step 5). After the completion of the fermentation, the fermented powder can be dried to obtain a water content of about 10% (Step 6) to complete the powdered fermented feed.

Example  4

The same procedure as in Example 3 was carried out, except that only the worms were dried in the first step. The second step to the sixth step, which will be described later, are carried out in the same manner as in Example 3 to prepare powdery feeds.

Comparative Example 1

20% by weight of worms, 20% by weight of worms, 30% by weight of fish meal and 30% by weight of sea tangle are mixed and dried at about 30 ° C to obtain a moisture content of 10%. The dried material is then pulverized to 120 mesh.

Distilled water is mixed with distilled water in a volume ratio of 1: 3 to the mixed material.

The material mixed with the distilled water was put into a fermenter for fermentation for 36 hours. After completion of the fermentation, the fermentation was completed and dried to obtain a water content of about 10%.

The experimental conditions were as shown in Table 1. The average length and the average weight of the sea cucumber seedlings and the first-year-old sea cucumbers after 45 weeks were 45 weeks, Respectively.

In addition, the survival rates of sea cucumbers raised by the diets of the examples and the comparative examples were compared.

Breeding period 45 weeks (conducted on September 1) Target of Breeding Sea cucumber seedling, 1 year old sea cucumber Experimental tank Water-based (adjusted to 4 L / min) Oxygen supply Air stone installation (5mg / L) Daily feeding rate Supply twice a day at 8:00 am and 6:00 pm Supply, 50 ~ 80g per m2

Sea cucumber seedlings (after 45 weeks) One-year-old sea cucumber (after 45 weeks) Size (cm) Weight (g) Size (cm) Weight (g) Example 1 8.1 48 18.3 380 Example 2 7.7 42 17.2 371 Example 3 8.5 50 18.9 408 Comparative Example 1 6.1 35 14.2 305

As a result, the sea cucumbers raised from the feeds of Examples 1 to 3 can grow fully within 2 years and can grow to be shipped. In addition, the sea cucumbers of the Examples had a growth rate of 20% ~ 35% more than that of Comparative Example 1, and the digestion and absorption rate was also much better due to the microbial fermentation feed.

In addition, the sea cucumber using the feeds of the Examples has a better survival rate, and has an effect of surviving about 25% more than the sea cucumber using the Comparative Example 1.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the inventions. It will be clear to those who have knowledge of.

Claims (9)

10-20% by weight of wormwood, 1-20% by weight of wormwood, 5-20% by weight of sea mustard, 5-20% by weight of kelp, 5-20% by weight of topsoil, 5-20% 5-20% by weight of diatomaceous earth, 10-30% by weight of soybean meal, and 5-20% by weight of microorganisms.
The present invention relates to a microbial fermentation feed for sea cucumber, which comprises 80 to 95% by weight of insect worms and 5 to 20% by weight of microorganisms.
3. The method according to claim 1 or 2,
The microorganism may be Rhodobacter sphaeroides, Bacillus subtilus, Lactobacillus sp., Pediococcus sp., Sacharomyces cerevisiae, Nitrobactor sp. And Nitrosomonas sp. Wherein the microbial fermented feed for sea cucumber is selected from the group consisting of:
3. The method according to claim 1 or 2,
Wherein the average size of the sea cucumber is increased by 20 to 35% during 45 weeks of fermentation using the fermented feed for sea cucumber.
10-20% by weight of wormwood, 1-20% by weight of wormwood, 5-20% by weight of sea mustard, 5-20% by weight of kelp, 5-20% by weight of topsoil, 5-20% 5-20% by weight of diatomaceous earth and 10-30% by weight of soybean meal;
A second step of pulverizing the mixed material;
Adding the microorganism to the pulverized material, further mixing 80 to 95% of the material and 5 to 20% by weight of the microorganism;
A fourth step of mixing distilled water into the mixture of the microorganisms; And
And fermenting the mixture in a fermenter to produce a fermented microorganism feed for sea cucumber.
6. The method of claim 5,
Wherein the microorganism fermented feed for sea cucumber is prepared by using only the dried worms in the first step or after washing them in a natural state.
The method according to claim 5 or 6,
And a sixth step of drying and pulverizing the further fermented mixture after the fifth step.
The method according to claim 5 or 6,
The microorganism may be Rhodobacter sphaeroides, Bacillus subtilus, Lactobacillus sp., Pediococcus sp., Sacharomyces cerevisiae, Nitrobactor sp. And Nitrosomonas sp. Wherein the microbial fermented animal feed is selected from the group consisting of:
The method according to claim 5 or 6,
Wherein the crushing is performed at 100-120 mesh in the second step.

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