KR100881886B1 - Method for producing fish meal and feed using thereof - Google Patents

Abstract

A method for preparing a fish meal, are a feed using the fish meal prepared by the method are provided to use the fish meal prepared with the steamed freshwater fish as an animal protein source in the feed. A method for preparing a fish meal comprises the steps of steaming a freshwater fish with steam; drying the steamed freshwater fish; mixing the dried freshwater fish with rice straw and fermenting it; and drying the fermented one and pulverizing it. Preferably the steamed freshwater fish is dried at 45-55 deg.C by using a hot wind drier to a water content of 15-20 wt%.

Description

Method for producing fish meal using freshwater fish and feed using the fish meal.

The present invention relates to a method of preparing fish meal using freshwater fish and feed using the fish meal, and more specifically, by using fermented freshwater fish with rice straw and using it as feed for animals and farmed fish, many freshwater fish resources can be utilized. To ensure that

Freshwater fish refers to fish that live in freshwater, and refers to fish species inhabiting rivers or upstream swamps and those living in dams and reservoirs.

The classification of freshwater fish in our country is divided into carp, loach, catfish, sesame, family, dental floss, willowfish, drungury, and trout.

On the other hand, currently used livestock feed grain is the main raw material, fish meal must be mixed to provide the animal protein necessary for the growth of the livestock. However, the fishmeal is not currently produced in Korea, and it is dependent on the total amount of imports.

Literature information of the prior art

Korean Patent Publication No. 10-1971-191

Korean Patent Publication No. 10-1973-73

Korean Patent Publication No. 10-1993-0001801

Therefore, an object of the present invention is to solve the problems of the conventional feed, to produce fish meal by fermenting steamed freshwater fish with rice straw, so that it can be used as an animal protein raw material in the feed.

In addition, an object of the present invention is to supplement the animal high protein and abundant microelements well digestible absorption and supply to the livestock and farmed fish at a low price to help the growth and immunity of the livestock and farmed fish, the farmhouse during fermentation of freshwater fish By using rice straw, which is a waste of resources, the manufacturing cost can be reduced.

Method for producing fish meal using freshwater fish according to the present invention for achieving the above object, the step of steaming freshwater fish with steam,

Drying the steamed freshwater fish;

Fermenting the dried freshwater fish with rice straw;

Drying and grinding the fermented fermented product;

It characterized in that it comprises the step of autoclaving the pulverized pulverized product.

In addition, the feed using fish meal is characterized in that it comprises 1 to 10% by weight of the prepared fish meal and 90 to 99% by weight of livestock feed.

In addition, the feed using fishmeal is characterized in that the mixture is made by mixing 80% to 95% by weight of fishmeal to 5 to 20% by weight of the binder.

According to the method for preparing fish meal using freshwater fish and the feed using the fish meal according to the present invention, by fermenting steamed freshwater fish with rice straw to prepare fish meal, it can be used as a raw material of animal protein in the feed, thereby reducing the import cost of feed. It is possible to supplement the animal's high protein and abundant trace elements with livestock and farmed fish, and to support the growth and immunity of livestock and farmed fish. By using it, a useful effect, such as making the manufacturing cost low, is provided.

The method for preparing fish meal according to the present invention comprises the step of fermenting steamed freshwater fish with rice straw.

That is, when freshwater fish is added to the feed without fermentation, problems arise in the supply and storage of freshwater fish, and in order to improve the problem that it is difficult to utilize due to poor digestion absorption even when ingesting livestock fish, steamed steamed freshwater fish By fermentation by using, it is possible to easily prepare fish meal, it is possible to produce livestock feed and farmed fish feed using the fish meal.

The rice straw is used as a source of Bacillus bacteria, since the rice straw is used as it is without separating the strain from rice straw for fermentation, the production method is simple and economical.

In the present invention, freshwater fish refers to a family of fish that inhabit fresh water.

Hereinafter, a method for preparing fish meal according to the present invention will be described.

Freshwater fish are collected and washed three times or more with purified water and tested for harmful metals and pesticides.

When the washing is completed, the freshwater fish is steamed with steam, which is steamed for 2 to 3 hours at a temperature of 90 to 100 ° C. At this time, the temperature is to consider the breaking point of the purified water and the heating time is to consider the steaming degree. Through the steaming process, the fermentation of freshwater fish will be easier.

When steaming is completed as described above, the steamed freshwater fish is dried to a water content of 15 to 20% by weight at 45 to 55 ° C. using a hot air dryer equipped with ultraviolet rays, etc., to artificially induce the production of vitamin D. This is to help the absorption of calcium when fish meal is used as a feed additive.

At this time, if the drying temperature is less than 45 ℃ drying time is long and if it exceeds 55 ℃ is too high temperature to dry at 45 ~ 55 ℃, if the moisture content is less than 15% or more than 20% by weight Fermentation as a treatment is poor.

When the drying is completed, the dried freshwater fish is fermented by mixing with rice straw, wherein the rice straw is used as a cell source of Bacillus bacteria.

In addition, the rice straw may be used as a source of natto bacteria, Moriyasito pilar bacteria and the like, in addition to Bacillus, it is used as one or two or more sources.

Furthermore, without adding rice straw, Bacillus, NATO, moshiyashito, and the like may be added alone, and if freshwater fish can be fermented, the method is not limited.

At this time, the reason for using the rice straw mainly, rice straw is enough to be used in the farmhouse, rice straw containing about 25 to 30% moisture can be washed clean without using a separate process to use as it is from the Bacillus straw Because the bacteria are not separated and used, they are easy to use, cost-effective, and have sufficient protein degradation capacity. At this time, rice straw is added to about 1 to 10 parts by weight based on 100 parts by weight of steamed freshwater fish, but is not limited thereto.

And the fermentation is performed for 26 to 96 hours at a temperature of 30 to 50 ℃, if the fermentation temperature is less than 30 ℃ or more than 50 ℃ cell growth does not occur because the fermentation is less than 26 hours, fermentation is This is because if it is not sufficiently made and exceeds 96 hours, the fermentation product will rot and cannot be used as a raw material for feed.

In the fermentation process, the cells decompose the protein, and the polymer is modified to a low molecular weight by changing the molecular structure. In other words, when used as a feed, it is easy to absorb digestion, and the bowel beneficial metabolism to improve bowel and metabolism to enhance the immune system to growth and disease. In addition, the freshwater fish originally contained minerals, potassium, phosphorus, magnesium, iron, vitamins, amino acids, which makes it a suitable material for feed.

When the fermentation is complete it is dried for 4 to 6 hours at 45 ~ 50 ℃ in a hot air dryer and pulverized it. At this time, the drying temperature or time is not necessarily limited, so as to properly adjust in consideration of the amount of fermentation, etc., the grinding also does not limit the size.

When the drying and grinding is completed, it is additionally autoclaved (0.7 to 1.3 Psi, 7 to 10 minutes) so that it can be safely used as feed. At this time, the autoclave method is not limited.

The fishmeal sealed through autoclaving can be stored for 24 months at room temperature under shading, and its distribution and storage are easy.

Hereinafter, a method of preparing a feed using fish meal of the present invention will be described.

First, when the fish meal according to the present invention is used as an animal feed, 1 to 10% by weight of fish meal and 95 to 99% by weight of livestock feed are used.

At this time, if the fish meal is less than 1% by weight, the animal protein is too small, so that the growth of the animal is not good, if it exceeds 10% by weight may cause imbalance with other nutrients, it is preferable to mix at 95 to 99% by weight Do.

Feed according to the present invention is to provide a high digestion and absorption of animal high protein and abundant microelements to promote the growth of livestock and enhance immunity to enhance disease resistance. Chickens will also increase egg production.

When the fish meal is used as a feed for fish farming, the fish meal may be used as it is, or the fish meal may be mixed with the fish meal to be used. The fish meal may be used by mixing 5 to 20 wt% of the binder with 80 to 95 wt% of the fish meal. do. At this time, the binder is for shaping the fish meal is less than 5% by weight because the effect is insignificant, if it exceeds 20% by weight is excessive.

And as the binder may be used one or a mixture of two or more selected from the group consisting of corn starch, PVP (PVP) and Siemsi (CMC), it is also possible to use a separate binder in addition to the binders listed above Of course.

In addition, the corn starch may be a luxury corn starch.

Hereinafter, the present invention will be described in detail through examples.

Example 1

The chicks (super herbivore-blood lineage) were bred for 10 days in general feed (commercially available feed), and then divided into 2 groups of 40 animals with constant weight. One group (test group) of the two groups were mixed with 10% by weight of fish meal of the present invention in a general feed, and the other group (control group) was bred as a general feed.

The chicks of the two groups were raised for 4 weeks to measure weight change and mortality. The results were as shown in Table 1 below.

Chick breeding test result division Test group (g) Control group (g) Weight gain (g) Start of test 69.4 69.4 0 1 week later 125.8 125.3 0.5 after 2 weeks 174.8 170.6 4.2 3 weeks later 218.6 213.5 5.1 4 weeks later 296.5 289.2 7.3 Our company 0 0 -

As can be seen in Table 1, the weight of the experimental group ingested fish meal of the present invention was found to be somewhat increased compared to the control group bred with the general feed, and to increase the immune system and increase the digestive function against growth promotion and disease Could expect.

In addition, due to the ingestion of calcium, which is rich and absorbed, it is expected that the laying rate of the mother chicken will be increased, the shell of the egg will be hard, and the breakage during transportation will be reduced and the immunity will be improved.

Example 2

The fish meal according to the present invention was mixed with the solid feed powder (Samyang Oil Feed Co., Ltd.) so as to be 0.5% by weight and 2% by weight, respectively.

Animals used in this example were ICR-based mice weighing 18-24g and SD-white mice weighing about 200g, respectively. Samtako Inc. was used as a solid feed of Samyang Oil Feed Co., Ltd. Use after adjusting to laboratory environment for 2 weeks with sufficient supply. Experiments were carried out at 24 ± 2 ℃ unless otherwise specified.

Six mice were used for 1 group, and each sample containing 0.5% and 2.0% was freely ingested for 4 weeks, and the control group was allowed to consume only feed. Body weight was measured twice a week at intervals of 3 to 4 days, and the results are shown in FIG. 1.

On the last day, weigh the body, lightly anesthetize with ether, collect blood by cardiac subtraction, separate serum according to the conventional method, and use aminotransferase activity (GOT & GPT) and triglyceride (TG) in serum. ) And total cholesterol (TC) content was measured and compared and the results are shown in Table 2 below. (Aminotransferase activity was measured by Reitaman & Frankel's method, TC content by enzyme method such as Allain, and TG content by enzyme method such as Saldesai.) And the experimental results are expressed as mean ± standard error.

Hematological component change test result division Transaminase (GOT) activity (IU / mL) (GPT) TC level (mg / dl) TG level (mg / dl) Control 74.8 ± 9.02 27.0 ± 0.75 153.0 ± 8.05 169.8 ± 12.9 Kill Group (0.5%) 55.5 ± 6.86 27.2 ± 2.92 145.3 ± 7.85 184.8 ± 28.0 Kill Group (2%) 61.7 ± 5.33 33.7 ± 3.63 136.5 ± 7.33 199.7 ± 27.0

First, as can be seen in Figure 1, the weight gain rate of the control group was increased over time, after 4 weeks was found to increase by about 24%. In addition, the sample mixed with 0.5% by weight was able to confirm the weight gain over time, and the increase rate after 4 weeks was 27.6% by weight, but the statistically significant difference was not recognized. Samples with high concentrations of 2% by weight also showed weight gain over time, and the weight gain rate at 4 weeks was 20.7%.

And as can be seen in Table 2, GOT enzyme activity tended to decrease somewhat in the sample treatment group compared to the control group, but there was no significant difference, GPT enzyme activity tended to increase slightly in the 2% by weight treatment group, but the significant difference was Not recognized. In addition, the TC content was slightly suppressed in the 2% by weight treatment group compared to the control group showed a tendency to increase the TG content slightly.

The effect on prolonged swimming time of the mice under heavy load was tested.

The method of Mororiura (T) etc. was improved. A 25 × 40 × 17 cm transparent plastic container was filled with distilled water up to 15 cm and placed in a 25 ° C. thermostatic bath to maintain a constant temperature. In the swimming experiment, we weighed and fixed the lead wire of 8% of body weight to the back part of the neck and took a swim 30 minutes after the oral administration.The end of the swimming was allowed to sink for 5 seconds until the nose was submerged. The end of the swim time was completed.

The results are shown in FIG. 2.

As can be seen in Figure 2, the swimming time of the control group was 13.1 ± 1.72 minutes, 0.5 wt% and 2.0 wt% treatment group showed a tendency to slightly extend to 17.4 ± 2.41 minutes and 15.3 ± 3.87 minutes, respectively.

Effects on Hematologic Indicators of Swimming Experiments in Heavy Load Rats

A plastic container of 35 cm in diameter and 35 cm in height was filled with distilled water up to 25 cm in height, and 6 rats were swimed for 3 hours in 6 swimming baths while maintaining constant water temperature at 25 ± 1 ° C. After swimming for 3 days, the rats weighed the rats, weighed 1% of the lead wire around their necks, and forced to swim one hour after the last oral dose of the last sample. Immediately after, blood glucose was measured and blood was collected without anesthesia. After blood collection, serum was obtained by centrifugation at 5 ° C. for 15 minutes at 3000 ° C. at 4 ° C., and then the creatinine, blood urea nitrogen (BUN) and lactic acid dehydrogenase (LDH) activities were measured. Table 3 shows the LDH activity measured by lactic acid substrate method, creatinine content by Jaffe method, and BUN content by using a kit reagent of Asan Pharmaceutical Co., Ltd. using urease indophenol method such as Fawcett and Prine Automatic Clinical Chemistry Analyzer. Glucose content was measured using life scan Co., One touch blood glucose meter.

Effect of test on prolonged swimming time in heavy load mice division Blood glucose level (mg / dl) Greatinine level (mg / dl) BUN level (mg / dl) LDH Activity (mg / dl) nomal 100.7 ± 2.81 0.71 ± 0.03 15.6 ± 1.52 858.3 ± 251.1 Control 34.5 ± 6.88 1.05 ± 0.07 21.5 ± 0.61 2641.7 ± 319.0 Kill Group (0.5%) 28.7 ± 3.32 1.16 ± 0.12 21.1 ± 2.02 3520.0 ± 242.8 Kill Group (2%) 30.7 ± 4.47 1.13 ± 0.08 17.8 ± 0.90 1496.7 ± 201.8

As can be seen in Table 3, the glucose, creatinine, BUN content and LDH enzyme activity in the control group was forced to change significantly compared to the normal group without the forced swimming was recognized. In the sample treatment group, the blood glucose content tended to decrease slightly, but no statistically significant difference was observed. Serum creatinine content was not significantly changed in the sample treatment group compared with the control group, but the BUN content in the 2 wt% treatment group was 17.8 ± 0.90 mg / dl compared to the control group 21.5 ± 0.61 mg / dl. It became. On the other hand, LDH enzyme activity was significantly increased in the 0.5 wt% treatment group and significantly decreased in the 2 wt% treatment group.

Although the present invention has been described with reference to the above embodiments, the present invention is not limited to the above embodiments, and various modifications can be made without departing from the scope and spirit of the present invention.

1 is a graph showing the weight change of the mouse.

Figure 2 is a graph showing the swimming time of the heavy load mice.

Claims (10)

Method for producing fish meal using freshwater fish, characterized in that comprising the step of fermenting steamed freshwater fish with rice straw. The method of claim 1, Steaming freshwater fish with steam, Drying the steamed freshwater fish; Fermenting the dried freshwater fish with rice straw; Drying and fermentation of the fermented fermented product The method for preparing fish meal using freshwater fish, characterized in that it comprises a step. The method of claim 2, The drying of the steamed freshwater fish is a method of preparing fish meal using freshwater fish, characterized in that the water content is dried at 15 to 20% by weight at 45 to 55 ℃ using a hot air dryer. The method of claim 2, The fermentation step of mixing the dried freshwater fish with rice straw, the production method of fish meal using freshwater fish, characterized in that the fermentation for 26 to 96 hours at a temperature of 30 ~ 50 ℃. The method of claim 2, After the pulverizing step, the method of manufacturing a fish meal using freshwater fish, characterized in that further comprising the step of autoclaving the pulverized crushed product. The method of claim 2, The rice straw is a method for preparing fish meal using freshwater fish, characterized in that it comprises one or two or more strains selected from the group consisting of Bacillus, Nato and Moriyasito. A feed using fish meal, characterized in that 1 to 10% by weight of fish meal prepared by the method of any one of claims 1 to 6 and 90 to 99% by weight of livestock feed are mixed. A feed using fish meal, characterized in that the binder is mixed with the fish meal prepared by the method of any one of claims 1 to 6, and then molded. The method of claim 8, Feed with fish meal, characterized in that for mixing the fish meal 80 to 95% by weight of the binder 5 to 20% by weight. The method of claim 8, The binder is a feed using fish meal, characterized in that one or two or more selected from the group consisting of corn starch, PVP (PVP) and Siemsi (CMC).

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