KR810001482B1 - Method of manufacturing the micro-organic protein feed - Google Patents
Method of manufacturing the micro-organic protein feed Download PDFInfo
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- KR810001482B1 KR810001482B1 KR1019800001415A KR800001415A KR810001482B1 KR 810001482 B1 KR810001482 B1 KR 810001482B1 KR 1019800001415 A KR1019800001415 A KR 1019800001415A KR 800001415 A KR800001415 A KR 800001415A KR 810001482 B1 KR810001482 B1 KR 810001482B1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/32—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from hydrolysates of wood or straw
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
Description
본 발명은 특허 제4879호 바실루스균 단백사료의 제조방법에 대한 발명특허를 연구 발전한 것으로서 발명특허 제4879호 내용중 균주 사용과 발효등 공정의 복잡성을 개선 보완한 균체 단백질사료 첨가물의 제조방법에 관한 것이다.The present invention is a research and development of the invention patent for the manufacturing method of Bacillus Bacillus protein feed of Patent No. 4879 relates to a method for producing a bacterial protein feed additive supplemented by improving the complexity of the process, such as the use of strains and fermentation in the content of Invention Patent No. 4879 will be.
본 발명은 볏짚류 또는 왕겨분을 대두박과 맥강분으로 된 알카리성 배지액 pH8-8.6)으로 혼련하고 여기에다 고온 섬유소분해 균주인 Bacillus stearo thermo philus균과 발효 공동 균주로 Bacillus megatherium균을 접종한 후 발효열을 점차적으로 승온 조절하여 25-65℃까지 상승 발효케하여 원료인 볏짚 또는 왕겨분의 기질 섬유소를 분해자화(分解資化)한 균체를 증식시켜 균체 단백질과 부수적으로 생성되는 각종 효소 등을 가금(家禽) 가축(家畜) 사료의 균체 단백질 첨가물로서 이용하는데 그 목적이 있다. 농산부산물로 다량 배출되는 볏짚류 및 왕겨등이 가축 동물들에 영양 공급원(供給源)으로서 가치가 없는 근본 원인은 본래가 이러한 저질의 조사료등이 다량 내포하고 있는 리구닝(Lignin) 펜토산(Pentosan) 등은 소화 효소기질인 탄수화물에 작용할 수 없겠금 방해 요소가 되고 있으며 규산질을 함유한 섬유소도 그 조직의 연결집합(連結集合)이 너무 단단하여 여기에도 소화 효소의 작용을 쉽게 받을 수 없게 되어 있다.The present invention kneads rice straw or chaff powder with alkaline medium solution pH8-8.6) consisting of soybean meal and pulverulum powder, and inoculated Bacillus megatherium bacteria with Bacillus stearo thermo philus bacterium and fermentation co-strain, which is a high-temperature fibrinolytic strain, followed by fermentation heat. Gradually increase the temperature to 25-65 ℃ fermentation to increase the degrading magnetization of the substrate fiber of rice straw or rice husk powder as raw material to grow the cell protein and various enzymes that are generated by the secondary poultry (家禽) Its purpose is to use it as a cell protein additive in livestock feed. Rice roots and rice hulls, which are produced as agricultural by-products, are of no value as a source of nutrients to livestock animals. The root cause is liginin pentosan, ) Is unable to act on carbohydrates, which are digestive enzyme substrates, and is a hindrance, and fiber-containing silicic acid is too hard to connect with the tissues so that the digestive enzymes cannot be easily affected.
전술한 바와같은 볏짚 또는 왕겨 등의 견고한 조직으로된 규산질 함유 섬유소 분해에 알카리성 배기액으로 혼련한 발효 기질에다 또한 알카리 내성과 섬유소 분해력이 강한 Bacillus stearo thermo philus균과 공동 발효 균주인 Bacillus megatherium균을 접종 발효시 종전방법에 비하여 볼 때 기질의 섬유소 분해와 균체 단백질의 증식이 현저하게 향상 발전된 것을 발견하게 된 것이다Inoculated with the fermentation substrate kneaded with alkaline exhaust liquid to the decomposition of siliceous-containing fibrin, such as straw straw or rice hull, as described above, and also the bacterium Bacillus stearo thermo philus, which has strong alkali resistance and fibrinolytic ability, and Bacillus megatherium, a co-fermentation strain. Compared to the previous method during fermentation, it was found that the fibrin breakdown of substrate and the proliferation of cell protein were significantly improved.
이것은 배지액중에 함유되어 있는 알카리 성분이 규산질(sio2) 섬유소의 규산 결제 조직 분해를 돕고 균체의 섬유소 용분해로 생성되는 물질을 자화하여 증식이 계속되는 것으로 보며 pH환경이 미세균과 효소생성에 강한 영향을 준 것으로 본다.It is believed that the alkali component contained in the medium liquid helps the siliceous settlement of silicic acid (sio 2 ) fibrin, and magnetizes the material produced by the cell lysis of cells. I think it affected.
본 발명을 상세히 설명하면 다음과 같다.The present invention is described in detail as follows.
본 발명에 있어 배지액 조성과 점차적인 발효온도 상승 요인이 발효 성과에 중요한 역할을 하는 것으로서 배지액 조성에 있어 세균 배양 증식에 필요한 질소원으로서 탈지대두박(脫脂大豆粕)과 탄소원으로서 맥강분을 사용하여 NaOH, NH4, Na2CO3등으로 배지액에 pH를 8-8.6으로 조성한 것에 발효 기제인 볏짚 또는 왕겨분말을 혼합, 혼련하면서 균주인 Bacillus stearo thermo philus균과 공동 발효균인 Bacillus megatherium균을 접종하고 함수율을 60-70%로 하여 발효하게 되는 것이다.In the present invention, the medium liquid composition and the gradual increase of the fermentation temperature play an important role in the fermentation performance. Inoculate the culture medium with NaOH, NH 4 , Na 2 CO 3 to pH 8-8.6 and mix and knead the fermented rice straw or rice husk powder while inoculating the strain Bacillus stearo thermo philus and Bacillus megatherium bacteria co-fermented. And fermentation at a moisture content of 60-70%.
발효 단계에서 Bacillus megatherium균은 생육 적온이 25-35℃이고, Bacillus stearo thermo philus균은 50-65℃가 생육 적온이므로 1차적으로 Bacillus megatherium균을 25-40℃까지 승온하여 발효를 지속하여 세균증식 및 효소를 생성토록 하고 2차적으로 Bacillus stearo thermo philus 균을 40-65℃에서 발효토록 점차적으로 승온케하여 1차 발효시에 분해 생성된 물질을 자화하여 가일층 세균의 증식 및 각종 효소 등을 생성토록 하며 또한 65℃이상의 고온을 기(旣)발효에서 생성된 균체의 열처리 원으로서 이용한다.In the fermentation stage, Bacillus megatherium bacteria grow at 25-35 ℃, and Bacillus stearo thermo philus bacteria grow at 50-65 ℃. And enzymatically generate and further increase the temperature of Bacillus stearo thermo philus bacteria at 40-65 ℃ for fermentation, and magnetize the decomposed product during the first fermentation to further grow bacteria and produce various enzymes. In addition, the high temperature of 65 ℃ or more is used as a heat treatment source of the cells produced by the fermentation.
본래 미생물 균체가 가진 강인한 세포벽은 단백 소화효소에 강한 저항성을 가지고 있어 가축에게 급여시 불 소화성이 될 뿐 아니라 세포 단백질의 대부분을 불소화(不消化) 미이용(未利用)되는 것이다.The inherent microbial cell walls have a strong resistance to protein digestive enzymes, which render them indigestible when fed to livestock, as well as fluoridating most of the cellular proteins.
균체를 50-70℃의 온도에서 가열처리가 행하여지며는 세균체가 변성하여 세포내의 유효성분이 세로외(外)의 추출이 용이해지며, 또한 단백 소화 효소 및 Nuclease에 작용을 받으며는 산 가용성(酸可溶性)의 저분자 핵산물질의 양이 많아지며 가축에게 급여시는 소화 흡수가 향상되는 것이다. 또한 발효시 혼입된 잡균 및 포자 등에 있어 사상균은 20-30℃ 병원균은 35-37℃, 효모균이 25-32℃가 최적온도 이므로 고온 발효균의 발효온도 65℃ 이상에서는 대부분 사멸되므로 본 발명 발효물에 저온균에 인한 변패를 막을 수 있어 장기간의 보존 저장이 가능하게 되는 것이다.When the cells are heated at a temperature of 50-70 ℃, the bacteria are denatured and the active ingredients in the cells are easily extracted outside of the cells.They are also soluble in protein digestion enzymes and nucleases. The amount of low molecular weight nucleic acid material is increased, and digestive absorption is improved when fed to livestock. In addition, filamentous bacteria mixed at the time of fermentation, such as filamentous bacteria at 20-30 ℃ pathogens 35-37 ℃, yeast bacteria 25-32 ℃ is the optimum temperature because most of the fermentation temperature of the high temperature fermentation bacteria at 65 ℃ or more, so the present invention It is possible to prevent the deterioration caused by low-temperature bacteria, which enables long-term preservation and storage.
이상과 같이 본 발명은 간편한 공정방법으로 염가(廉價)의 사료 첨가물 생산이 가능하여 양축업계에 널리 보급할 수 있는 장점이 있다.As described above, the present invention has a merit that can be widely distributed in the livestock industry because it is possible to produce inexpensive feed additives by a simple process method.
실시예를 들면 다음과 같다.An example is as follows.
제1공정 : 볏짚, 왕겨를 30-80매슈(mash)로 분쇄한다.First step: crush rice straw and rice husk into 30-80 mash.
제2공정 : 배지액 조성으로 청수 600ℓ에 탈지 대두박 10-15kg, 맥강분 5-10kg을 침지(浸漬) 교반(攪伴)하여 수분 흡수가 끝나면 마쇄기(磨碎機)로서 마쇄한 다음 자비부(煮沸釜)에 투입하고 NaOH, NH4OH, Na2CO3등으로 배지액 pH가를 8-8.6으로 조정한 후 가열 자비하여 발효에 필요한 배지액을 얻는다.Second step: immerse and stir 10-15kg of defatted soybean meal and 5-10kg of pulverized soybean meal in 600L of fresh water as a medium solution composition (Iii) The pH of the medium is adjusted to 8-8.6 with NaOH, NH 4 OH, Na 2 CO 3 and the like, followed by heating to obtain a medium for fermentation.
제3공정 : 30-80매슈로 분쇄된 볏짚 또는 왕겨분 500kg을 자비 완료한 배지액과 함수율 60-70%로 하여 혼련하면서 발효 균주인 Bacillus stearo thermo philus 균과 발효 공동균으로 Bacillus megatherium균을 동량비(同量比)로 적당량을 균일하게 접종하여 발효실에서 품은 25℃로 부터 점차적으로 자체 발효열로서 승온 케하여 마지막에 65℃로 하고 30시간 통기발효(通氣醱酵)를 지속한 다음 40-50℃에 온풍건조를 거쳐 본 발명에 의한 제품을 얻는다.The third step: 500kg of rice straw or rice husk crushed with 30-80 mesh was kneaded with a medium solution of 60% to 70% water content, and Bacillus megatherium bacteria were fermented and Bacillus megatherium bacteria were fermented by the same ratio. Inoculate an appropriate amount uniformly, and gradually increase the temperature of the fermentation room from 25 ° C. The hot air drying is carried out to obtain the product according to the present invention.
배지액으로 혼련된 원료기질의 균 접종이 끝난 이후에 발효진행은 중(中) 온균인 Bacillus megatherium 균에 의한 발효 개시기로 3시간 경과시는 온도 상승과 발효 냄새등으로 균증식을 인지할 수 있으며 8-10시간 경가시는 대수 증식기에 들어가며 온도는 40℃전후로 상승하게 되며 15시간 경과 후 부터 발효열이 급상승한다.After inoculation of the raw material substrates kneaded with the medium solution, fermentation is started by Bacillus megatherium, which is a moderate temperature bacterium. It enters the logarithmic growth stage at 8-10 hours, and the temperature rises around 40 ℃ and the heat of fermentation increases rapidly after 15 hours.
이 시점가지 Amylase, Protease등 각종 효소 생성 및 세균증식이 왕성함을 알 수 있다.At this point, various enzymes such as Amylase and Protease are produced and bacterial growth is vigorous.
20시간이 되면서 50-60℃의 발효열이 발생하며 이 시기부터 Bacillus stearo thermo philus균의 활동기에 들어가며 기제의 분해 및 효소생성이 가일층 왕성함을 볼 수 있다.The fermentation heat of 50-60 ℃ is generated in 20 hours, and from this time, Bacillus stearo thermo philus bacteria are active, and the decomposition of enzymes and the formation of enzymes are very active.
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| KR1019800001415A KR810001482B1 (en) | 1980-04-04 | 1980-04-04 | Method of manufacturing the micro-organic protein feed |
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| KR1019800001415A KR810001482B1 (en) | 1980-04-04 | 1980-04-04 | Method of manufacturing the micro-organic protein feed |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100738007B1 (en) * | 2005-11-22 | 2007-07-13 | 부경대학교 산학협력단 | -68 Novel Bacillus Velezensis A-68 and Use of the Same |
| KR100882021B1 (en) * | 2006-12-13 | 2009-02-04 | 부경대학교 산학협력단 | Method for production of Bacillus velezensis A-68 with rice hull |
| KR100881886B1 (en) * | 2008-01-31 | 2009-02-06 | 주식회사 경희매니지먼트컴퍼니 | Method for producing fish meal and feed using thereof |
| KR101357174B1 (en) * | 2010-11-30 | 2014-02-03 | 건국대학교 산학협력단 | Novel method of fermenting rice husk and composition comprising fermented rice husk therefrom |
-
1980
- 1980-04-04 KR KR1019800001415A patent/KR810001482B1/en not_active IP Right Cessation
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100738007B1 (en) * | 2005-11-22 | 2007-07-13 | 부경대학교 산학협력단 | -68 Novel Bacillus Velezensis A-68 and Use of the Same |
| KR100882021B1 (en) * | 2006-12-13 | 2009-02-04 | 부경대학교 산학협력단 | Method for production of Bacillus velezensis A-68 with rice hull |
| KR100881886B1 (en) * | 2008-01-31 | 2009-02-06 | 주식회사 경희매니지먼트컴퍼니 | Method for producing fish meal and feed using thereof |
| KR101357174B1 (en) * | 2010-11-30 | 2014-02-03 | 건국대학교 산학협력단 | Novel method of fermenting rice husk and composition comprising fermented rice husk therefrom |
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