KR102489946B1 - 글루코스-의존성 미토콘드리아 호흡 및 2-상 인슐린 분비 반응을 나타내는 인간 만능 줄기 세포 유래 기능성 베타 세포의 생성 - Google Patents

글루코스-의존성 미토콘드리아 호흡 및 2-상 인슐린 분비 반응을 나타내는 인간 만능 줄기 세포 유래 기능성 베타 세포의 생성 Download PDF

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KR102489946B1
KR102489946B1 KR1020227008775A KR20227008775A KR102489946B1 KR 102489946 B1 KR102489946 B1 KR 102489946B1 KR 1020227008775 A KR1020227008775 A KR 1020227008775A KR 20227008775 A KR20227008775 A KR 20227008775A KR 102489946 B1 KR102489946 B1 KR 102489946B1
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KR20220039833A (ko
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세바스티앙 리크
알리레자 레자니아
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얀센 바이오테크 인코포레이티드
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KR1020227008775A 2016-06-21 2017-06-14 글루코스-의존성 미토콘드리아 호흡 및 2-상 인슐린 분비 반응을 나타내는 인간 만능 줄기 세포 유래 기능성 베타 세포의 생성 Active KR102489946B1 (ko)

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KR1020237001597A KR102607813B1 (ko) 2016-06-21 2017-06-14 글루코스-의존성 미토콘드리아 호흡 및 2-상 인슐린 분비 반응을 나타내는 인간 만능 줄기 세포 유래 기능성 베타 세포의 생성

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US201662352968P 2016-06-21 2016-06-21
US62/352,968 2016-06-21
KR1020187037170A KR102376911B1 (ko) 2016-06-21 2017-06-14 글루코스-의존성 미토콘드리아 호흡 및 2-상 인슐린 분비 반응을 나타내는 인간 만능 줄기 세포 유래 기능성 베타 세포의 생성
PCT/US2017/037373 WO2017222879A1 (en) 2016-06-21 2017-06-14 Generation of human pluripotent stem cell derived functional beta cells showing a glucose-dependent mitochondrial respiration and two-phase insulin secretion response

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KR20220039833A KR20220039833A (ko) 2022-03-29
KR102489946B1 true KR102489946B1 (ko) 2023-01-18

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Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3638205A4 (en) 2017-06-14 2021-03-17 Semma Therapeutics, Inc. DEVICES AND METHODS FOR ADMINISTERING THERAPEUTICS
US20200199540A1 (en) * 2017-09-11 2020-06-25 Novo Nordisk A/S Enrichment of nkx6.1 and c-peptide co-expressing cells derived in vitro from stem cells
CN118497104A (zh) 2017-11-15 2024-08-16 沃泰克斯药物股份有限公司 胰岛细胞制备性组合物和使用方法
EP3758719A4 (en) * 2018-03-02 2021-12-08 Vertex Pharmaceuticals Inc. METHODS FOR IMPROVING THE DIFFERENTIATION OF STEM CELLS INTO BETA CELLS
WO2019208788A1 (ja) * 2018-04-27 2019-10-31 株式会社カネカ 膵臓β細胞の製造方法
CN112867497A (zh) * 2018-05-07 2021-05-28 哈佛学院校长同事会 与体外β细胞分化相关的细胞群和基因表达
EP3790956A4 (en) * 2018-05-11 2022-03-16 Cornell University METHOD FOR INDUCING DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS INTO MONOHORMONAL CELLS
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
US10724052B2 (en) 2018-09-07 2020-07-28 Crispr Therapeutics Ag Universal donor cells
JP7555975B2 (ja) 2019-05-31 2024-09-25 ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド 生体適合性メンブレン複合体
WO2020243666A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
JP2022534545A (ja) 2019-05-31 2022-08-01 ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド 生体適合性メンブレン複合体
CA3257300A1 (en) 2019-05-31 2025-06-12 W. L. Gore & Associates, Inc. Cell encapsulation devices with controlled oxygen diffusion distances
WO2020264072A1 (en) * 2019-06-25 2020-12-30 Semma Therapeutics, Inc. Enhanced differentiation of beta cells
JP7696301B2 (ja) * 2019-06-27 2025-06-20 ダナ－ファーバー キャンサー インスティテュート，インコーポレイテッド タンパク質分解のための化合物、組成物、および方法
AU2020340622A1 (en) 2019-09-05 2022-03-03 Crispr Therapeutics Ag Universal donor cells
US20230014430A1 (en) * 2019-11-20 2023-01-19 Washington University Methods and Compositions for Generating Functionally Mature Beta Cells and Uses Thereof
WO2021233116A1 (zh) * 2020-05-21 2021-11-25 北京大学 多能干细胞的成分明确、无异源的培养系统
EP4647080A3 (en) 2020-07-31 2026-02-18 Vertex Pharmaceuticals Incorporated Differentiation of pancreatic endocrine cells
KR20230146007A (ko) 2020-12-31 2023-10-18 크리스퍼 테라퓨틱스 아게 범용 공여자 세포
CN114958726B (zh) * 2021-02-23 2025-03-21 海门雨霖细胞科技有限责任公司 化学诱导成纤维细胞重编程为肺干细胞的化学小分子组合物及其应用
WO2022204377A1 (en) * 2021-03-24 2022-09-29 Salk Institute For Biological Studies NOVEL CHEMICAL COMBINATIONS AND METHODS OF USE THEREOF, TOWARDS DIFFERENTIATION OF HUMAN PROGENITOR CELLS INTO FUNCTIONAL β CELLS
US20250114355A1 (en) * 2021-05-07 2025-04-10 Mayo Foundation For Medical Education And Research Treating liver disease
US20220403340A1 (en) * 2021-06-11 2022-12-22 Washington University Media for cryopreserved cells and methods of making and using same
AU2022377078A1 (en) 2021-11-01 2024-04-04 Vertex Pharmaceuticals Incorporated Stem cell derived pancreatic islet differentiation
CN114317401B (zh) * 2021-12-07 2022-10-04 浙江大学 一种促进胰岛前体细胞长期稳定传代的方法
KR102848180B1 (ko) * 2021-12-15 2025-08-19 한국화학연구원 오로라 키나아제 억제제를 이용하는 심근세포 및 심장 오가노이드의 제조방법
US20240034996A1 (en) * 2022-07-29 2024-02-01 Therapeutic Solutions International, Inc. Gene modified ipsc derived cellular compositions for regeneration and immune modulation
WO2025117331A1 (en) 2023-12-01 2025-06-05 Eli Lilly And Company Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same
WO2025119299A1 (en) * 2023-12-08 2025-06-12 Hangzhou Reprogenix Bioscience, Inc. Method for differentiating pancreatic beta cells
WO2025202874A1 (en) 2024-03-25 2025-10-02 Crispr Therapeutics Ag Genetically modified cells expressing glp-1 receptor agonist
WO2026069163A1 (en) * 2024-09-25 2026-04-02 Crispr Therapeutics Ag Differentiation method for producing immature beta cells

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
ES2564044T3 (es) 2003-06-27 2016-03-17 DePuy Synthes Products, Inc. Células posparto derivadas de tejido del cordón umbilical y métodos de preparación y uso de las mismas
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US8586357B2 (en) * 2003-12-23 2013-11-19 Viacyte, Inc. Markers of definitive endoderm
JP4819697B2 (ja) 2003-12-23 2011-11-24 ヴィアサイト，インコーポレイテッド 胚体内胚葉
ES2387402T3 (es) 2005-03-31 2012-09-21 Stemnion, Inc. Composiciones celulares derivadas del amnios, procedimientos de fabricación y usos de las mismas
AU2006202209B2 (en) 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
US7807459B2 (en) 2005-09-27 2010-10-05 Reneuron, Inc. EphA4-positive human adult pancreatic endocrine progenitor cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US20090269313A1 (en) * 2006-07-19 2009-10-29 Diakine Therapeutics, Inc. Encapsulation system
AU2007277364B2 (en) 2006-07-26 2010-08-12 Viacyte, Inc. Methods of producing pancreatic hormones
ATE523585T1 (de) 2007-11-27 2011-09-15 Lifescan Inc Differenzierung menschlicher embryonaler stammzellen
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
US8728812B2 (en) 2008-04-22 2014-05-20 President And Fellows Of Harvard College Compositions and methods for promoting the generation of PDX1+ pancreatic cells
KR101651661B1 (ko) 2008-06-30 2016-08-26 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
EP2318516A1 (en) 2008-06-30 2011-05-11 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
CN102272291B (zh) 2008-10-31 2018-01-16 詹森生物科技公司 人胚胎干细胞向胰腺内分泌谱系的分化
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
CN102695790A (zh) 2009-10-29 2012-09-26 詹森生物科技公司 多能干细胞
AU2010333839C1 (en) 2009-12-23 2016-07-21 Janssen Biotech Inc. Differentiation of human embryonic stem cells
KR101877077B1 (ko) 2010-08-09 2018-07-10 다케다 야쿠힌 고교 가부시키가이샤 췌호르몬-생성 세포의 제조 방법
US9388386B2 (en) 2011-12-22 2016-07-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
WO2013184888A1 (en) * 2012-06-08 2013-12-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
JP6470687B2 (ja) 2012-09-03 2019-02-13 ノヴォ ノルディスク アー／エス 小分子を用いた多能性幹細胞からの膵臓内胚葉の作製
US9902938B2 (en) * 2012-12-26 2018-02-27 Kyoto University Method for inducing exocrine pancreatic cells
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
RU2684215C2 (ru) 2012-12-31 2019-04-04 Янссен Байотек, Инк. Способ получения панкреатических эндокринных клеток (варианты) и способ увеличения выхода бета-клеток
WO2014105543A1 (en) 2012-12-31 2014-07-03 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
US20140329704A1 (en) * 2013-03-28 2014-11-06 President And Fellows Of Harvard College Markers for mature beta-cells and methods of using the same
KR102580225B1 (ko) * 2013-06-11 2023-09-20 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β 세포 및 조성물 그리고 그 생성 방법
JP6588969B2 (ja) 2014-05-16 2019-10-09 ヤンセン バイオテツク，インコーポレーテツド 膵内分泌細胞内のｍａｆａ発現を強化するための小分子の使用
JP2019506153A (ja) * 2016-01-08 2019-03-07 マサチューセッツ インスティテュート オブ テクノロジー 分化した腸内分泌細胞およびインスリン産生細胞の作製
US11274280B2 (en) * 2016-02-24 2022-03-15 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Cell, vol.159(2), pp.428~439(2014)*

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