KR102458490B1 - 핵산 정제 카트리지 - Google Patents

핵산 정제 카트리지 Download PDF

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Publication number
KR102458490B1
KR102458490B1 KR1020177008858A KR20177008858A KR102458490B1 KR 102458490 B1 KR102458490 B1 KR 102458490B1 KR 1020177008858 A KR1020177008858 A KR 1020177008858A KR 20177008858 A KR20177008858 A KR 20177008858A KR 102458490 B1 KR102458490 B1 KR 102458490B1
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filter
port
cavity
microfluidic device
flow path
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Korean (ko)
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KR20170045339A (ko
Inventor
포라스 프란세스크 베니테즈
고메즈 조셉 파레자
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스타트-다이아그노스티카 앤드 이노베이션, 에스.엘.
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/146—Employing pressure sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0622—Valves, specific forms thereof distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
KR1020177008858A 2014-09-03 2015-09-02 핵산 정제 카트리지 Active KR102458490B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14183411.9 2014-09-03
EP14183411.9A EP2992958A1 (en) 2014-09-03 2014-09-03 Nucleic acid purification cartridge
PCT/EP2015/070045 WO2016034620A1 (en) 2014-09-03 2015-09-02 Nucleic acid purification cartridge

Publications (2)

Publication Number Publication Date
KR20170045339A KR20170045339A (ko) 2017-04-26
KR102458490B1 true KR102458490B1 (ko) 2022-10-26

Family

ID=51518547

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020177008858A Active KR102458490B1 (ko) 2014-09-03 2015-09-02 핵산 정제 카트리지

Country Status (12)

Country Link
US (1) US10793849B2 (enExample)
EP (2) EP2992958A1 (enExample)
JP (1) JP6800144B2 (enExample)
KR (1) KR102458490B1 (enExample)
CN (1) CN106999928B (enExample)
AU (1) AU2015310942B2 (enExample)
BR (1) BR112017004191B1 (enExample)
CA (1) CA2959954C (enExample)
ES (1) ES2873362T3 (enExample)
RU (1) RU2722075C2 (enExample)
WO (1) WO2016034620A1 (enExample)
ZA (1) ZA201702211B (enExample)

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WO2012135805A2 (en) 2011-03-31 2012-10-04 modeRNA Therapeutics Delivery and formulation of engineered nucleic acids
US11377470B2 (en) 2013-03-15 2022-07-05 Modernatx, Inc. Ribonucleic acid purification
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
EP2971033B8 (en) 2013-03-15 2019-07-10 ModernaTX, Inc. Manufacturing methods for production of rna transcripts
EP3052511A4 (en) 2013-10-02 2017-05-31 Moderna Therapeutics, Inc. Polynucleotide molecules and uses thereof
US10286086B2 (en) 2014-06-19 2019-05-14 Modernatx, Inc. Alternative nucleic acid molecules and uses thereof
WO2016011222A2 (en) 2014-07-16 2016-01-21 Moderna Therapeutics, Inc. Circular polynucleotides
US12109274B2 (en) 2015-09-17 2024-10-08 Modernatx, Inc. Polynucleotides containing a stabilizing tail region
US11434486B2 (en) 2015-09-17 2022-09-06 Modernatx, Inc. Polynucleotides containing a morpholino linker
WO2017223176A1 (en) * 2016-06-24 2017-12-28 Modernatx, Inc. Methods and apparatus for filtration
GB201703383D0 (en) 2017-03-02 2017-04-19 Gargle Tech Ltd Testing for particulates
KR102065649B1 (ko) * 2017-12-28 2020-01-13 에스디 바이오센서 주식회사 핵산 추출용 카트리지의 피스톤
EP4205851A3 (en) 2018-09-05 2023-10-04 Hero Scientific Ltd Testing for particulates
GB201819417D0 (en) * 2018-11-29 2019-01-16 Quantumdx Group Ltd Vacuum-assisted drying of filters in microfluidic systems
EP3986602A4 (en) * 2019-06-19 2023-08-02 Merck Sharp & Dohme LLC CONTAINMENT SYSTEM FOR MIXING DRY POWDER WITH SOLVENTS DURING PHARMACEUTICAL MANUFACTURE OR PROCESSING
US20210063291A1 (en) * 2019-08-30 2021-03-04 Boehringer Ingelheim Vetmedica Gmbh Filter instrument, kit and method for pretreating a sample
WO2021181339A1 (en) 2020-03-11 2021-09-16 Hero Scientific Ltd. Testing devices
CN111545256B (zh) * 2020-03-31 2022-05-13 深圳市刚竹医疗科技有限公司 离心微流控芯片
CA3202405A1 (en) 2021-01-06 2022-07-14 Zvi Feldman Filtration sampling devices
CN112899132B (zh) * 2021-01-13 2021-10-15 上海金鑫生物科技有限公司 一种用于核酸提取与纯化的硅胶过滤吸头
CN119875797B (zh) * 2025-03-28 2025-07-08 湖南远泰生物技术有限公司 质粒dna滤纸洗脱组件、自动微流控系统及洗脱方法

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JP2002345465A (ja) 2001-05-24 2002-12-03 Fuji Photo Film Co Ltd 核酸精製ユニット及び核酸精製方法
KR100486402B1 (ko) 1996-09-18 2005-04-29 프로메가 코포레이션 필터 조립체 및 핵산 물질 분리 방법
JP2012517020A (ja) 2009-02-03 2012-07-26 ネットバイオ・インコーポレーテッド 核酸精製

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FR2657543B1 (fr) * 1990-01-26 1992-12-18 Biocom Sa Dispositif modulaire pour le recueil, l'incubation, la filtration d'echantillons multiples.
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KR100486402B1 (ko) 1996-09-18 2005-04-29 프로메가 코포레이션 필터 조립체 및 핵산 물질 분리 방법
JP2002345465A (ja) 2001-05-24 2002-12-03 Fuji Photo Film Co Ltd 核酸精製ユニット及び核酸精製方法
JP2012517020A (ja) 2009-02-03 2012-07-26 ネットバイオ・インコーポレーテッド 核酸精製

Also Published As

Publication number Publication date
EP2992958A1 (en) 2016-03-09
BR112017004191B1 (pt) 2022-07-05
US10793849B2 (en) 2020-10-06
JP6800144B2 (ja) 2020-12-16
WO2016034620A1 (en) 2016-03-10
RU2722075C2 (ru) 2020-05-26
KR20170045339A (ko) 2017-04-26
EP3188838B1 (en) 2021-04-14
JP2017532016A (ja) 2017-11-02
AU2015310942A1 (en) 2017-04-20
CN106999928B (zh) 2021-05-18
AU2015310942B2 (en) 2021-02-04
ZA201702211B (en) 2018-08-29
ES2873362T3 (es) 2021-11-03
CN106999928A (zh) 2017-08-01
EP3188838A1 (en) 2017-07-12
US20170283792A1 (en) 2017-10-05
BR112017004191A2 (pt) 2017-12-12
RU2017110274A3 (enExample) 2019-02-22
CA2959954A1 (en) 2016-03-10
CA2959954C (en) 2022-07-19
RU2017110274A (ru) 2018-10-03

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