KR102448016B1 - Composition for preventing or improving stretch marks comprising complex extract as active ingredient - Google Patents

Abstract

The present invention relates to a composition for preventing or alleviating stretch marks, which comprises: a gromwell root extract, an angelica gigas root extract, a glycyrrhiza uralensis root extract, a scutellaria baicalensis root extract, a sophora flavescens root extract, a poncirus trifoliata fruit extract, a forsythia suspensa fruit extract, a tangerine peel extract, a bird's nest extract and phosvitin phosphopeptides (PPPs) as active ingredients, and optionally comprises fructan or corn germ extract as active ingredients. The composition for preventing or alleviating stretch marks, according to the present invention, greatly improves the elasticity of the skin around the stretch marks by the synergistic action of the medicinal plant complex extract, bird's nest extract, and phosvitine phosphopeptide, which are active ingredients, and greatly relieves stretch marks. In addition, when the composition for preventing or alleviating stretch marks, according to the present invention, further includes fructan or corn germ extract as an active ingredient, the effect of improving skin elasticity and relieving stretch marks is maximized.

Description

Composition for preventing or improving stretch marks comprising complex extract as active ingredient}

The present invention relates to a composition for preventing or improving stretch marks, and more particularly, to a composition that is safe for the human body and can inhibit or alleviate stretch marks by including a natural product-based complex extract component as an active ingredient.

Stretch marks are commonly called stretch marks, and in medical terminology they are called striae distensae or dilated striatum. The first histological verification of stretch marks was performed by Elson in 1889. According to him, stretch marks are a type of skin disease in which lines similar to scars appear on the skin. At first, it is red in color, but gradually the surface changes to a shiny white color, and overall skin becomes harder than normal skin. The morphological definition of stretch marks was made by Nadelli in 1936, who named it 'striae atrophicae'. There is little known about the exact treatment method of hyperthyroidism (Acta Derm Venereol Suppl. 59, 161-169, 1979). The external symptom of stretch marks is that the epidermis of the skin is slightly submerged and appears in the form of microscopic scars. It is often accompanied by a little itching in the early stage of development, and it is a common phenomenon that the skin becomes wrinkled and thin, and gradually increases in length as time passes and the color becomes red. At this time, when you touch the stretch marks with your hands, it is said that unlike normal skin, the skin does not have elasticity, so it feels like jelly. In a shiny state, it leaves a scar that contrasts sharply with the surrounding skin color. As such, the red, thin line form of stretch marks is called striae rubra, and the form of white lines with dents in the epidermis is called striae alba. These stretch marks refer to a condition in which collagen, which acts as a support for the skin, and elastin and fibrillin, which form elasticity, are cut or reduced due to various factors, resulting in cosmetic abnormalities in the skin. In other words, it refers to the occurrence of scars in the form of lines or bands due to the expansion and stretching of the skin for some reason.

Causes of stretch marks include rapid physical growth in adolescence, weight gain, pregnancy and increase in adrenal corticosteroids in the body. As muscle or fat increases rapidly, it appears on the epidermis of the abdomen or chest. In addition, the dilated striatum that appears in adolescents in the growing stage occurs mainly around the arms, legs, and groin as the skin cannot keep up with the growth rate of the musculoskeletal body due to the rapid body growth rate of puberty.

As described above, dermatological treatment is the most common treatment for stretch marks that occur for various reasons, but dermatological treatment is focused on treating stretch marks in the early stages, and it does not show any special effect in the late stage when white striatum is generated. I have a problem that I can't.

Meanwhile, the cosmetic industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. Natural ingredients have fewer side effects on the skin, and their development value as a cosmetic raw material is gradually increasing as consumers' response to cosmetics using natural ingredients increases. For example, Korean Patent Publication No. 10-2084635 includes an extract of Mealworm Brown (Tenebrio molitor) as an active ingredient, and the extract of Mealworm Brown is included in an amount of 001 to 10,000 ppm based on the total weight of the stretch mark prevention composition. An anti-stretch mark composition is disclosed. In addition, Korean Patent Publication No. 10-2051417 discloses a cosmetic composition for preventing and improving stretch marks, characterized in that it contains a complex extract of lotus leaf, persimmon leaf, centella leaf, camphor leaf, bay leaf, bokbunja, cheonnyeoncho and bupyeongcho as an active ingredient. has been disclosed. In addition, Republic of Korea Patent No. 10-1854444 discloses a cosmetic composition for preventing and improving stretch marks comprising an artichoke leaf extract, a cinnamon extract, and a gooseberry extract as active ingredients. In addition, Republic of Korea Patent Publication No. 10-1924356 contains green tea extract, Ecklonia cava extract, and Byeong pine needle extract as active ingredients, and the green tea extract, Ecklonia cava extract and Byeong pine needle extract are used in a weight ratio of 1: 0.1 to 1.5: 0.1 to 1.5. There is disclosed a composition for preventing or improving skin stretch marks, comprising:

The present invention has been derived from the prior technical background, and an object of the present invention is to provide a novel composition that is safe for the human body and can inhibit the occurrence of stretch marks or alleviate stretch marks by including a natural product-based complex extract component as an active ingredient. is in

In order to solve the above object, an example of the present invention is Gromwell (Scientific name: Lithospermum erythrorhizon) root extract, Angelica gigas root extract, Licorice (Glycyrrhiza uralensis) root extract, Golden (Scutellaria baicalensis) root extract , Sophora flavescens root extract, Poncirus trifoliata fruit extract, Forsythia suspensa fruit extract, tangerine peel extract, swallow's nest extract and phosvitin phosphopeptides (PPPs) as active ingredients. It provides a composition for preventing or improving stretch marks.

In order to solve the above object, an example of the present invention is Gromwell root extract, Angelica gigas root extract, Licorice (Glycyrrhiza uralensis) root extract, Golden (Scutellaria baicalensis) root extract, Sophora flavescens ) root extract, Poncirus trifoliata fruit extract, Forsythia suspensa fruit extract, tangerine peel extract, swallow's nest extract, phosvitin phosphopeptides (PPPs) and fructan as active ingredients It provides a composition for preventing or improving stretch marks.

In order to solve the above object, an example of the present invention is Gromwell root extract, Angelica gigas root extract, Licorice (Glycyrrhiza uralensis) root extract, Golden (Scutellaria baicalensis) root extract, Sophora flavescens ) Stretch marks containing root extract, Poncirus trifoliata fruit extract, Forsythia suspensa fruit extract, mandarin peel extract, swallow's nest extract, phosvitin phosphopeptides (PPPs) and corn germ extract as active ingredients A composition for prevention or improvement is provided.

The composition for preventing or improving stretch marks according to the present invention can greatly improve the elasticity of the stretch marks area skin and greatly relieve the stretch marks area by the synergistic action of the medicinal plant complex extract, swallow's nest extract and phosvitin phosphopeptide as active ingredients. In addition, when the composition for preventing or improving stretch marks according to the present invention further contains fructan or corn germ extract (recognized raw material for functional health functional food) as an active ingredient, the effect of improving the skin elasticity of the stretch marks and alleviating the stretch marks is maximized

Hereinafter, the present invention will be described in detail.

The present invention relates to a novel composition that is safe for the human body and can suppress or alleviate skin stretch marks by including natural product-based ingredients as active ingredients.

The composition for preventing or improving skin stretch marks according to an embodiment of the present invention is Gromwell root extract, Angelica gigas root extract, licorice (Glycyrrhiza uralensis) root extract, Golden (Scutellaria baicalensis) root extract, ginseng ( Sophora flavescens root extract, Poncirus trifoliata fruit extract, Forsythia suspensa fruit extract, tangerine peel extract, swallow's nest extract and phosvitin phosphopeptides (PPPs) are included as active ingredients. In addition, the composition for preventing or improving skin stretch marks according to an embodiment of the present invention is preferably Gromwell root extract, Angelica gigas root extract, Glycyrrhiza uralensis root extract, gold (Golden) as an active ingredient. In addition to Scutellaria baicalensis root extract, Sophora flavescens root extract, Poncirus trifoliata fruit extract, Forsythia suspensa fruit extract, tangerine peel extract, swallow's nest extract and phosvitin phosphopeptides (PPPs) It may further include fructan or corn germ extract.

The Gromwell (Scientific name: Lithospermum erythrorhizon or Lithospermum officinale) is a dicotyledonous perennial plant of the Lamiaceae family, called 'Chichi' in oriental medicine, and its root is called 'self-inflicted'.

The Angelica gigas is a succulent plant of the umbel family of the dicotyledonous bicotyledonous plant group, and in the present invention, the Chinese scientific name 'Angelica sinensis' and the Japanese 'Werodangwi' are included.

The licorice (Glycyrrhiza uralensis) is a dicotyledonous perennial plant of the Rosaceae legume family, and in the present invention, the scientific name 'Glycyrrhiza glabra var. glandulifera' and the Spanish scientific name 'Glycyrrhiza glabra'.

The gold (Scutellaria baicalensis) is a dicotyledonous perennial plant of the order Lamiaceae, and the root is used in oriental medicine as an antipyretic, diuretic, antidiarrheal, dandruff and anti-inflammatory agent.

The old ginseng (Sophora flavescens) is a dicotyledonous perennial plant of the Rosaceae legume, also called 'the thief's stick', and includes the scientific name 'Sophora angustifolia' in the present invention.

The tangja (Poncirus trifoliata fruit) refers to the yellow fruit of the deciduous broad-leaved arboretum of the Rutaceae taengja tree (Poncirus trifoliata).

The forsythia (Forsythia suspensa) is a plant belonging to the genus Forsythia, and its fruit is called 'Yeonkyo' in oriental medicine, and includes Forsythia viridissima in the present invention.

The tangerine peel is also called 'Dinpi' in oriental medicine and refers to the mature rind of a tangerine (scientific name: Citrus unshiu or Citrus reticulata) of the Unhyang family.

The swallow's nest is a dried piece of nest made by swallowing seaweed gelatinous material and mixing saliva.

The phosvitin phosphopeptides (PPPs) are oligopeptides obtained by hydrolyzing phosvitin with a proteolytic enzyme, and are known to have superior calcium solubilizing ability and calcium binding ability compared to phosvitin. In addition, it has been reported that phosvitin phosphopeptides (PPPs) have iron-binding ability, antioxidant activity, and the like, like phosvitin. In general, peptides or protein fragments having biochemical activity are those whose parent protein is decomposed into about 4 to 20 amino acids in length during digestion in vivo. Accordingly, peptides obtained by decomposing various proteins into appropriate sizes or modified peptides thereof tend to have high activity compared to the parent protein or to have various activities such as blood pressure enhancement, antioxidant, cholesterol lowering, anticancer, antibacterial, and the like. Phosvitin, one of the proteins present in eggs, is a phosphoglycoprotein of about 35 kDa composed of 177 amino acids and accounts for about 7% of egg yolk protein. About 10% of phosphorus is bound to phosvitin, and the amount of phosphorus bound to phosvitin is about 80% of the phosphorus contained in the total egg yolk. The high phosphorus content is because phosphorus is bonded to a serine residue that accounts for about 40% of amino acids constituting phosvitin. Phosphorus bound to a serine residue may form a soluble complex by chelating bonds with divalent metal ions such as Fe ²⁺ , Ca ²⁺ and Mg ²⁺ . In addition, phosvitin is known to have antibacterial or antioxidant activity.

The fructan is a generic term for oligosaccharides or polysaccharides in which several fructose residues are bonded to one glucose molecule, and is known to help moisturize the skin and enhance skin elasticity.

In the present invention, the Gromwell root extract, Angelica gigas root extract, Glycyrrhiza uralensis root extract, Scutellaria baicalensis root extract, Sophora flavescens root extract, Poncirus trifoliata fruit ) extract, Forsythia suspensa fruit extract, mandarin peel extract, swallow's nest extract, corn germ extract can be prepared using a conventional extraction method known in the art, for example, a solvent extraction method. The extraction solvent that can be used when preparing the medicinal plant extract, bird's nest extract, and corn germ extract by using the solvent extraction method is water, a lower alcohol having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol and butanol) or these It may be selected from the group consisting of hydrous lower alcohols, propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, and mixtures thereof, which are mixtures of , it is preferably selected from water, alcohol or a mixture thereof. When water is used as the extraction solvent, the water is preferably hot water. In addition, when alcohol is used as the extraction solvent, the alcohol is preferably a lower alcohol having 1 to 4 carbon atoms, and the lower alcohol is more preferably selected from methanol or ethanol. In addition, when a hydrous alcohol is used as the extraction solvent, the alcohol content is preferably 50 to 90%. The medicinal plant extract, bird's nest extract, and corn germ extract obtained by the solvent extraction method are filtered and recovered in the form of a filtered extract, concentrated by a vacuum evaporator, etc. and recovered in the form of a concentrated extract, freeze-dried or spray-dried It can be recovered in the form of extract powder by the solidification method of

In addition, the bird's nest extract of the present invention may be a hydrolysis product by heat treatment, acid treatment or enzyme treatment in addition to the extract obtained by solvent extraction, which is a common extraction method known in the art. In the present invention, with respect to the swallow's nest extract, reference is made to the contents of hydrolysis extraction of sea swallow's nest disclosed in paragraphs [0050] to [0059] of Korean Patent Registration No. 10-1257381. Bird's nest extract is known to have a wrinkle-improving effect.

In addition, the phosvitin phosphopeptides (PPPs) of the present invention are hydrolysis products obtained by hydrolyzing phosvitin with a proteolytic enzyme, and have a mass distribution within the range of 500 Da to 6 kDa, preferably 500 It has a mass distribution within the range of Da to 2 kDa, and most preferably has a mass distribution within the range of 500 Da to 1.5 kDa. In this case, the phosvitin hydrolyzate has the amino acid sequence of SEQ ID NO: 1, the number of bound phosphorus is 3 and the number of bound calcium is 6, The number of phosphorus bound with the amino acid sequence of SEQ ID NO: 2 is 3, and the number of bound phosphorus is 3 A phosvitin phosphopeptide having the number of formed calcium of 6, a phosvitin phosphopeptide having the amino acid sequence of SEQ ID NO: 3, having 3 bound calcium and 6 bound calcium, and a phosphorus bound with the amino acid sequence of SEQ ID NO: 4 The phosvitin phosphopeptide having the number of 4 and the number of bound calcium is 8, the phosvitin phosphopeptide having the amino acid sequence of SEQ ID NO: 5, the number of bound phosphorus is 8 and the number of bound calcium is 14, and the amino acid sequence of SEQ ID NO: 6. and at least one phosvitin phosphopeptide selected from the group consisting of phosvitin phosphopeptides having 10 bound phosphorus and 18 bound calcium. The phosvitin phosphopeptide used in the present invention is a liquid form containing phosvitin phosphopeptide as a main component, a concentrated liquid form containing phosvitin phosphopeptide as a main component, and freeze-drying or spray-drying the liquid or concentrated form It can have various forms, such as a solid form solidified into powder or granules by the method of

In addition, the corn germ extract of the present invention may be a public health functional food raw material (recognition number: 2014-8 No. 2014.02.19). The corn pear extract announced as a raw material for the health functional food is a raw material that has been recognized for its functionality to help moisturize the skin.

In the composition for preventing or improving skin stretch marks according to an embodiment of the present invention, the content of the active ingredient may be 0.1 to 12% by weight, preferably 0.2 to 10% by weight, more preferably based on the total weight of the composition. may be 0.5 to 6% by weight. Specifically, the composition for preventing or improving skin stretch marks according to an embodiment of the present invention contains 0.01 to 0.6% by weight of Gromwell root extract, 0.01 to 0.6% by weight of Angelica gigas root extract, and licorice based on the total weight. (Glycyrrhiza uralensis) root extract 0.01-0.6 wt%, Golden (Scutellaria baicalensis) root extract 0.01-0.6 wt%, Sophora flavescens root extract 0.01-0.6 wt%, Poncirus trifoliata fruit extract 0.01-0.6 wt% , 0.01-0.6% by weight of Forsythia suspensa fruit extract, 0.01-0.6% by weight of orange peel extract, 0.01-2.5% by weight of bird's nest extract, and 0.01-2.5% by weight of phosvitin phosphopeptides (PPPs) are effective It may be included as a component, preferably, based on the total weight, Gromwell root extract 0.02-0.4 wt%, Angelica gigas root extract 0.03-0.4 wt%, Licorice (Glycyrrhiza uralensis) root extract 0.02 ~0.4 wt%, Golden (Scutellaria baicalensis) root extract 0.02~0.4 wt%, Sophora flavescens root extract 0.02~0.4 wt%, Poncirus trifoliata fruit extract 0.02~0.4 wt%, Forsythia suspensa It may contain 0.02 to 0.4% by weight of fruit extract, 0.02 to 0.4% by weight of tangerine peel extract, 0.02 to 2% by weight of swallow's nest extract, and 0.02 to 2% by weight of phosvitin phosphopeptides (PPPs) as active ingredients. In addition, the composition for preventing or improving skin stretch marks according to an example may further include 0.01 to 2% by weight of fructan or 0.01 to 2% by weight of corn germ extract as an active ingredient, preferably based on the total weight. and more preferably, 0.02 to 1.5% by weight of fructan or 0.02 to 1.5% by weight of corn germ extract, based on the total weight, may be further included as an active ingredient.

The composition for preventing or improving skin stretch marks of the present invention may be a cosmetic composition. The cosmetic composition for preventing or improving skin stretch marks of the present invention may include a cosmetically acceptable carrier in addition to the active ingredient. For example, fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, emulsifiers, fillers, sequestering agents, chelating agents, preservatives, Vitamins, blocking agents, wetting agents, oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles, and the like, but are not limited thereto, and may include all known substances applicable to the cosmetic composition. In addition, the cosmetic composition for preventing or improving skin stretch marks of the present invention is a softening lotion, nourishing lotion, astringent lotion, skin, lotion, essence, cream, massage cream, pack, makeup base, BB cream, foundation, powder, cleansing foam, cleansing It may be formed in any one formulation selected from the group consisting of cream and cleansing water, but is not limited thereto, and may be formed in any known formulation. On the other hand, when the cosmetic composition for preventing or improving skin stretch marks of this embodiment having the above formulation is formed in a paste, cream or gel state, animal oil, vegetable oil, wax, paraffin, starch, tragaganth gum, cellulose derivative , polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, and the like may be further included. When the cosmetic composition for preventing or improving skin stretch marks of the present invention is formed in the form of a solution or emulsion, it may further include a solvent, a solubilizer, an emulsifier, and the like. The solvent, solubilizer, and emulsifier include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, and sorbitan. fatty acid esters and the like. When the cosmetic composition for preventing or improving skin stretch marks of the present invention is formed in a suspension form, a liquid diluent such as water, ethanol, propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, etc. of a suspending agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, tragaganth gum, and the like may be further included. When the cosmetic composition for preventing or improving skin stretch marks of the present invention is a cleansing foam, cleansing cream or cleansing water, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate , sarcosinate-based compound, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerol fatty acid ester, and the like may be further included.

The composition for preventing or improving skin stretch marks of the present invention may be an external composition. The external composition for preventing or improving skin stretch marks of the present invention is a warning agent (Plasters), lotions (Lotions), liniments (Liniments), liquids (Liquids and Solutions), aerosols (Aerosols), extracts (Extracts), Selected from the group consisting of Ointments, Fluidextracts, Emulsions, Suspensions, Capsules, Creams, Soft or hard gelatin capsules, Patches and sustained release formulations. It can be formed in any one of the formulations. The composition for external application for preventing or improving skin stretch marks of the present invention includes, in addition to the active ingredient, a pharmaceutically acceptable carrier, vehicle, excipient, binder, grinding agent, lubricant, colorant, etc., in addition to fragrance, preservative, stabilizer, dissolution Adjuvants or additives such as adjuvants and transdermal absorption accelerators may be further added. The binder may include starch, gum tragacanth, gelatin, molasses, polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose, carboxymethyl cellulose, and the like. The grinding agent may include agar, starch, gelatin powder, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, crystalline cellulose, calcium carbonate, sodium hydrogen carbonate, sodium alginate, and the like. The lubricant may include magnesium stearate, talc, hydrogenated vegetable oil, and the like. The vehicle and excipient may include lactose, glucose, sucrose, mannitol, potato starch, corn starch, calcium carbonate, calcium phosphate, cellulose, and the like. On the other hand, the standard dosage for the composition for external application of the present invention may vary depending on the individual characteristics of the patient, and a substantially skilled clinical doctor can provide an ideal dosage and administration for the composition for external application for skin of the present invention for a patient. A plan, for example, the most appropriate treatment strategy in view of the specific needs and the patient's overall condition, can be established. In addition, an appropriate dosage of the composition for external application for skin of the present invention may be generally determined in vitro or in an animal model. For example, an appropriate dosage may be determined by adding various concentrations of the composition for external application for skin of the present invention to target cells in vitro.

Hereinafter, the present invention will be described in more detail through examples. However, the following examples are only for clearly illustrating the technical features of the present invention, and do not limit the protection scope of the present invention.

Example 1: Preparation of test materials

(1) Zaungo Extract

Gromwell (Lithospermum erythrorhizon) root extract, Angelica gigas root extract, Glycyrrhiza uralensis root extract, Golden (Scutellaria baicalensis) root extract, Sophora flavescens root extract, Tangja (Poncirus trifoliata fruit) extract, Forsythia suspensa fruit extract, and tangerine peel extract were commercially available products. The Gromwell (Lithospermum erythrorhizon) root extract, Angelica gigas root extract, Licorice (Glycyrrhiza uralensis) root extract, Golden (Scutellaria baicalensis) root extract, Sophora flavescens root extract, Poncirus trifoliata fruit ) extract, Forsythia suspensa fruit extract, and tangerine peel extract were all mixed in equal amounts to prepare a Jaungo extract.

(2) Swallow's nest extract

500 g of swallow's nest was washed with purified water and dried to prepare a blue bird's nest sample. The prepared sample was pulverized and refined to obtain a powder. After adding 10 times the volume of 50% ethanol to the dry powder, reflux extraction was performed at 60° C. for 6 hr, followed by filtration, and the supernatant was concentrated with a vacuum concentrator and freeze-dried to prepare a powdery bird's nest extract.

(3) phosvitin phosphopeptide

Acidic water corresponding to about 9 times the volume of the egg yolk was added and mixed to prepare an aqueous egg yolk solution having a final pH of 5.0 to 5.2. After that, the aqueous egg yolk solution was left at 4° C. for about 12 hours without agitation, and then the supernatant containing rivetin was removed by first centrifugation at 6000 rpm for about 20 minutes, and the first precipitate was collected. did. The collected primary precipitate was washed twice with 95% ethanol to remove lipids, and the secondary precipitate was collected by secondary centrifugation. A sodium bicarbonate (NaHCO ₃ ) buffer having a pH of 9.6 was added to the collected secondary precipitate and homogenized with a homogenizer. The homogenized secondary precipitate was subjected to a third centrifugation at 6000 rpm for about 20 minutes to remove the precipitate containing lipovitellin, and the supernatant containing phosvitin was collected. The supernatant was passed through an anion exchange chromatography apparatus (Bio-Rad, Hercules, CA) to obtain purified phosvitin. At this time, the column of the anion exchange chromatography apparatus was pre-equilibrated with a 0.05M carbonate-bicarbonate buffer having a pH of 9.6. In addition, buffer A [a 0.05M carbonate-bicarbonate buffer with a pH of 9.6] and buffer B [a buffer in which sodium chloride is present at a concentration of 0.5M in buffer A) were supplied at a flow rate of about 2 ml/min. The supernatant injected into an anion exchange chromatography apparatus was eluted. Specifically, the ratio of buffer B was increased from 0% to 45% during the elution time corresponding to the 6 bed volume of the column, and then the ratio of buffer B was increased to 75% during the elution time corresponding to the 20 bed volume of the column. . The eluate was monitored by measuring the absorbance at 280 nm.

An aqueous solution of phosvitin was prepared by adding 0.2 parts by weight of phosvitin purified by the above protocol to 100 parts by weight of water and stirring. Thereafter, the pH of the aqueous solution of phosvitin was adjusted to 6.0, and proteolytic enzymes thermolysin, trypsin and elastase were added thereto in an amount corresponding to 2 parts by weight based on 100 parts by weight of phosvitin, respectively, and an ultra-high pressure machine (trade name: TFS-2L; manufacturer: Innoway, Korea), a primary hydrolyzate containing phosvitin phosphopeptide was prepared by performing a primary enzymatic hydrolysis reaction at a pressure of 100 MPa and a temperature of 50° C. for 18 hours. To the first hydrolyzate, trypsin, a proteolytic enzyme, was added in an amount corresponding to 2 parts by weight based on 100 parts by weight of phosvitin, and 100 MPa was added using an ultra-high pressure machine (trade name: TFS-2L; manufacturer: Innoway, Korea). A secondary hydrolyzate containing phosvitin phosphopeptide was prepared by performing a secondary enzymatic hydrolysis reaction for 24 hours under a pressure condition and a temperature condition of 50°C. The secondary hydrolyzate was freeze-dried to obtain phosvitin phosphopeptide.

The mass distribution of phosvitin phosphopeptide was measured for the secondary hydrolyzate using Matrix Assisted Laser Desorption and Ionization-Time of Flight (MALDI-TOF) mass spectrometry. As a result, it was found that the phosvitin phosphopeptide contained in the secondary hydrolyzate had a mass distribution range of 100 to 6000 Da (eg, 460 to 5860 Da). Table 1 below shows the MALDI-TOF (Matrix Assisted Laser Desorption and Ionization-Time of Flight) mass spectrometry results for the secondary hydrolyzate as a percentage of the mass range and the corresponding peak area. The main peaks appeared at 702.78 Da, 2529.29 Da, 5097.43 Da and 5627.873 Da, and the percentage of the main peak area to the total peak area was 8%, 9%, 8% and 22% based on the preceding order.

Mass range of phosvitin phosphopeptide peak area Percentage based on peak area (%) 500-1000 Da 2951 29.4 1000-2000 Da 1002 10.0 2000-3000 Da 1793 17.9 3000-6000 Da 4275 42.7

After dissolving the lyophilized secondary hydrolyzate in a mobile phase, phosvitin phosphopeptide was fractionated by molecular weight size using size exclusion chromatography. Specifically, the hydrolyzate dissolved in the mobile phase was passed through a size exclusion peptide column, and fractions were obtained in descending order of molecular weight size while eluting an amount corresponding to 1.5 to 2.5 times the column volume. Partial fractions corresponding to phosvitin phosphopeptide having a molecular weight of about 500 Da to 1.5 kDa among the obtained fractions were analyzed for amino acid sequences using an amino acid sequencer. Amino acid sequences and mass sizes of phosvitin phosphopeptides obtained by fractionation are shown.

Separation of phosvitin phosphopeptide fractions SEQ ID NO: amino acid sequence Mass size (Da) fraction 1 One KSSSN 522 fraction 2 2 HSSSH 554 fraction 3 3 RSSSK 564 fraction 4 4 KSSSSR 601 fraction 5 5 DSSSSSSSSV 929 fraction 6 6 RSSSSSSSSSSSR 1288

(4) fructan

A commercially available product was used for fructan.

(5) Corn Germ Extract

The corn germ was dried and then pulverized using a grinder for about 30 seconds to prepare a pulverized product. Thereafter, 2 kg of ground corn germ and 4 kg of 70% ethanol were mixed, extracted under reflux at 40° C. for 12 hr, filtered, and the supernatant was concentrated with a reduced pressure concentrator and freeze-dried to prepare a powdery corn germ extract.

Example 2: Preparation of cream formulation cosmetic composition

Cream formulation cosmetic compositions were prepared based on the components and compounding ratios of Tables 3 and 4 below.

Ingredients (wt%) Preparation Example 1 Preparation 2 Preparation 3 Comparative Preparation Example 1 active ingredient Zaungo Extract 0.4 0.3 0.3 1.2 swallow's nest extract 0.4 0.3 0.3 - phosvitin phosphopeptide 0.4 0.3 0.3 - fructan - 0.3 - - Corn Germ Extract - - 0.3 - 1,3-butylene glycol 2.8 2.8 2.8 2.8 glycerin 2.8 2.8 2.8 2.8 Hydrogenated Lecithin 1.0 1.0 .0 1.0 Octyldodecanol 2.8 2.8 2.8 2.8 trioctanoin 2.0 2.0 2.0 2.0 Stearic Acid 1.5 1.5 1.5 1.5 cetostearyl alcohol 2.0 2.0 2.0 2.0 Polysorbate 60 1.5 1.5 1.5 1.5 Sorbitan sesquioleate 2.0 2.0 2.0 2.0 dimethicone 2.8 2.8 2.8 2.8 antioxidant 0.3 0.3 0.3 0.3 Xanthan Gum 0.2 0.2 0.2 0.2 triethanolamine 0.1 0.1 0.1 0.1 EDTA 0.1 0.1 0.1 0.1 Purified water to 100 to 100 to 100 o 100

Ingredients (wt%) Comparative Preparation Example 2 Comparative Preparation Example 3 Comparative Preparation Example 4 Comparative Preparation Example 5 active ingredient Zaungo Extract - - - - swallow's nest extract 1.2 - - - phosvitin phosphopeptide - 1.2 - - fructan - - 1.2 - Corn Germ Extract - - - 1.2 1,3-butylene glycol 2.8 2.8 2.8 2.8 glycerin 2.8 2.8 2.8 2.8 Hydrogenated Lecithin 1.0 1.0 .0 1.0 Octyldodecanol 2.8 2.8 2.8 2.8 trioctanoin 2.0 2.0 2.0 2.0 Stearic Acid 1.5 1.5 1.5 1.5 cetostearyl alcohol 2.0 2.0 2.0 2.0 Polysorbate 60 1.5 1.5 1.5 1.5 Sorbitan sesquioleate 2.0 2.0 2.0 2.0 dimethicone 2.8 2.8 2.8 2.8 antioxidant 0.3 0.3 0.3 0.3 Xanthan Gum 0.2 0.2 0.2 0.2 triethanolamine 0.1 0.1 0.1 0.1 EDTA 0.1 0.1 0.1 0.1 Purified water to 100 to 100 to 100 o 100

Example 3: Measurement of skin elasticity improvement effect of cream formulation cosmetic composition

In order to measure the skin elasticity improvement effect of the cream-formed cosmetic composition prepared in Preparation Examples 1 to 3 and Comparative Preparation Examples 1 to 5, 80 subjects (females aged 20 to 40 years) were grouped into 8 groups of 10 people each. , and for each group, the cream-form cosmetic composition was applied to the skin stretch marks twice a day for 1 month in a row. In order to confirm the skin elasticity improvement effect, the viscoelasticity of the cream-formed cosmetic composition before and after use for 2 months was measured using a skin elasticity meter (cutometer SEM 575, C+K Electronic Co., Germany). Experimental results are described as R7 values of Cutometer SEM 575, and the average values of each group are shown in Table 5 below. The R7 value represents the property of viscoelasticity of the skin (n=10, p<0.05).

Classification of cream formulation cosmetic composition R7 Preparation Example 1 0.62 Preparation 2 0.69 Preparation 3 0.66 Comparative Preparation Example 1 0.32 Comparative Preparation Example 2 0.41 Comparative Preparation Example 3 0.24 Comparative Preparation Example 4 0.43 Comparative Preparation Example 5 0.45

As shown in Table 5, the cream-formed cosmetic composition of Preparation Examples 1 to 3 significantly improved skin elasticity in the stretch marks area, and these results were synergistic by the combination of Jaungo extract, swallow's nest extract and phosvitin phosphopeptide. It is based on action, and when fructan or corn germ extract (a functional food certified raw material for health functional food) is additionally used in combination, the skin elasticity improvement effect is further improved.

Example 4: Measurement of stretch marks improvement effect of cream formulation cosmetic composition

In order to measure the stretch marks improvement effect of the cream-formed cosmetic composition prepared in Preparation Examples 1 to 3 and Comparative Preparation Examples 1 to 5, 80 experimenters (20-40 years old female) were divided into 8 groups of 10 people each. Divided, and for each group, the cream-formed cosmetic composition was applied to the skin stretch marks twice a day for 4 consecutive weeks. The effect of improving stretch marks was evaluated by measuring the change in length (mm) of stretch marks over time after application of the cream. In Table 6 below, the length of stretch marks before applying the cream formulation cosmetic composition to the skin is converted to 100, and the stretch marks measured at 1 week, 2 weeks, 3 weeks, and 4 weeks, respectively, are converted to the length of 0 weeks. It was.

Classification of cream formulation cosmetic composition Stretch marks length by time (mm) Stretch marks improvement effect after 4 weeks 0 weeks 1 week 2 weeks 3 weeks 4 weeks Preparation Example 1 100 92.8 88.2 85.3 83.7 16.3% Preparation 2 100 90.6 85.4 81.6 79.5 21.5% Preparation 3 100 91.2 86.1 82.7 81.2 18.8% Comparative Preparation Example 1 100 96.6 94.5 92.9 91.6 8.4% Comparative Preparation Example 2 100 96.7 94.3 92.6 91.2 8.8% Comparative Preparation Example 3 100 98.7 97.2 96.4 95.8 4.2% Comparative Preparation Example 4 100 96.1 93.9 91.5 90.2 9.8% Comparative Preparation Example 5 100 96.1 93.8 91.1 89.9 10.1%

As shown in Table 6, the cream-formed cosmetic composition of Preparation Examples 1 to 3 remarkably relieved stretch marks, and these results were based on the synergistic action of the combination of Jaungo extract, swallow's nest extract and phosvitin phosphopeptide. In addition, when fructan or corn germ extract (a functional food certified raw material for health functional food) is additionally used in combination, the effect of relieving stretch marks is further improved.

As described above, the present invention has been described through the above embodiments, but the present invention is not necessarily limited thereto, and various modifications are possible without departing from the scope and spirit of the present invention. Accordingly, the protection scope of the present invention should be construed to include all embodiments falling within the scope of the claims appended hereto.

<110> thelifeb Co., Ltd. <120> Composition for preventing or improving stretch marks comprising complex extract as active ingredient <130> ZDP-21-0360 <160> 6 <170> KopatentIn 2.0 <210> 1 <211> 5 <212> PRT <213> Phosphopeptide derived from phosvitin of egg yolk <400> 1 Lys Ser Ser Ser Asn 1 5 <210> 2 <211> 5 <212> PRT <213> Phosphopeptide derived from phosvitin of egg yolk <400> 2 His Ser Ser Ser His 1 5 <210> 3 <211> 5 <212> PRT <213> Phosphopeptide derived from phosvitin of egg yolk <400> 3 Arg Ser Ser Ser Lys 1 5 <210> 4 <211> 6 <212> PRT <213> Phosphopeptide derived from phosvitin of egg yolk <400> 4 Lys Ser Ser Ser Ser Ser Arg 1 5 <210> 5 <211> 10 <212> PRT <213> Phosphopeptide derived from phosvitin of egg yolk <400> 5 Asp Ser Ser Ser Ser Ser Ser Ser Ser Val 1 5 10 <210> 6 <211> 13 <212> PRT <213> Phosphopeptide derived from phosvitin of egg yolk <400> 6 Arg Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Arg 1 5 10

Claims (4)

Based on the total weight, 0.02-0.4 wt% of Gromwell root extract, 0.03-0.4 wt% of Angelica gigas root extract, 0.02-0.4 wt% of Glycyrrhiza uralensis root extract, Scutellaria baicalensis Root extract 0.02-0.4 wt%, Sophora flavescens root extract 0.02-0.4 wt%, Poncirus trifoliata fruit extract 0.02-0.4 wt%, Forsythia suspensa fruit extract 0.02-0.4 wt%, tangerine peel A composition comprising 0.02 to 0.4% by weight of an extract, 0.02 to 2% by weight of a bird's nest extract, and 0.02 to 2% by weight of phosvitin phosphopeptides (PPPs) as active ingredients,
The phosvitin phosphopeptides (PPPs) is a hydrolysis product obtained by hydrolyzing phosvitin with a proteolytic enzyme and having a mass distribution within the range of 500 Da to 6 kDa, a composition for preventing or improving stretch marks .
The method of claim 1, wherein the phosvitin phosphopeptides (PPPs) have the amino acid sequence of SEQ ID NO: 1, the number of bound phosphorus is 3 and the number of bound calcium is 6, A phosvitin phosphopeptide having an amino acid sequence, having 3 bound phosphorus and 6 bound calcium, a phosvitin phosphopeptide having the amino acid sequence of SEQ ID NO: 3, having 3 bound phosphorus and 6 bound calcium, A phosvitin phosphopeptide having the amino acid sequence of SEQ ID NO: 4, wherein the number of bound phosphorus is 4 and the number of bound calcium is 8; A phosphopeptide comprising at least one phosvitin phosphopeptide selected from the group consisting of a phosphopeptide and a phosvitin phosphopeptide having the amino acid sequence of SEQ ID NO: 6 and having 10 bound phosphorus and 18 bound calcium A composition for preventing or improving stretch marks.
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