KR102428464B1 - 세포에 물질을 주입하여 세포를 변환시키는 방법 - Google Patents
세포에 물질을 주입하여 세포를 변환시키는 방법 Download PDFInfo
- Publication number
- KR102428464B1 KR102428464B1 KR1020150178183A KR20150178183A KR102428464B1 KR 102428464 B1 KR102428464 B1 KR 102428464B1 KR 1020150178183 A KR1020150178183 A KR 1020150178183A KR 20150178183 A KR20150178183 A KR 20150178183A KR 102428464 B1 KR102428464 B1 KR 102428464B1
- Authority
- KR
- South Korea
- Prior art keywords
- passage
- cells
- cell
- stem cells
- electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims description 88
- 239000000463 material Substances 0.000 title claims description 71
- 210000004027 cell Anatomy 0.000 claims abstract description 166
- 239000000126 substance Substances 0.000 claims abstract description 57
- 239000012530 fluid Substances 0.000 claims abstract description 19
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 18
- 239000007787 solid Substances 0.000 claims abstract description 16
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 12
- 241000700605 Viruses Species 0.000 claims abstract description 10
- 239000002105 nanoparticle Substances 0.000 claims abstract description 7
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 7
- 102000008186 Collagen Human genes 0.000 claims abstract description 5
- 108010035532 Collagen Proteins 0.000 claims abstract description 5
- 210000003855 cell nucleus Anatomy 0.000 claims abstract description 5
- 229920001436 collagen Polymers 0.000 claims abstract description 5
- 239000003814 drug Substances 0.000 claims abstract description 5
- 229940079593 drug Drugs 0.000 claims abstract description 5
- 210000002472 endoplasmic reticulum Anatomy 0.000 claims abstract description 5
- 210000002288 golgi apparatus Anatomy 0.000 claims abstract description 5
- 150000002484 inorganic compounds Chemical class 0.000 claims abstract description 5
- 229910010272 inorganic material Inorganic materials 0.000 claims abstract description 5
- 210000003712 lysosome Anatomy 0.000 claims abstract description 5
- 230000001868 lysosomic effect Effects 0.000 claims abstract description 5
- 210000003470 mitochondria Anatomy 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 210000003705 ribosome Anatomy 0.000 claims abstract description 5
- 150000002894 organic compounds Chemical class 0.000 claims abstract description 4
- 210000000130 stem cell Anatomy 0.000 claims description 32
- 230000010307 cell transformation Effects 0.000 claims description 15
- 210000004700 fetal blood Anatomy 0.000 claims description 12
- 108020004414 DNA Proteins 0.000 claims description 11
- 210000002919 epithelial cell Anatomy 0.000 claims description 11
- 238000011426 transformation method Methods 0.000 claims description 11
- 239000013612 plasmid Substances 0.000 claims description 9
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 6
- 230000003169 placental effect Effects 0.000 claims description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 5
- 210000004504 adult stem cell Anatomy 0.000 claims description 5
- 210000000349 chromosome Anatomy 0.000 claims description 5
- 210000001082 somatic cell Anatomy 0.000 claims description 5
- 108090000994 Catalytic RNA Proteins 0.000 claims description 4
- 102000053642 Catalytic RNA Human genes 0.000 claims description 4
- 108090000288 Glycoproteins Proteins 0.000 claims description 4
- 102000003886 Glycoproteins Human genes 0.000 claims description 4
- 108090001030 Lipoproteins Proteins 0.000 claims description 4
- 102000004895 Lipoproteins Human genes 0.000 claims description 4
- 210000004102 animal cell Anatomy 0.000 claims description 4
- 108091092562 ribozyme Proteins 0.000 claims description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 3
- 108020005544 Antisense RNA Proteins 0.000 claims description 3
- 108020004459 Small interfering RNA Proteins 0.000 claims description 3
- 239000003184 complementary RNA Substances 0.000 claims description 3
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 3
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 3
- 210000004602 germ cell Anatomy 0.000 claims description 3
- 229920002674 hyaluronan Polymers 0.000 claims description 3
- 229960003160 hyaluronic acid Drugs 0.000 claims description 3
- 239000002773 nucleotide Substances 0.000 claims description 3
- 125000003729 nucleotide group Chemical group 0.000 claims description 3
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 3
- 239000002924 silencing RNA Substances 0.000 claims description 3
- 210000003954 umbilical cord Anatomy 0.000 claims description 3
- 241000203069 Archaea Species 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 claims description 2
- 210000001789 adipocyte Anatomy 0.000 claims description 2
- 210000001691 amnion Anatomy 0.000 claims description 2
- 210000004381 amniotic fluid Anatomy 0.000 claims description 2
- 210000001185 bone marrow Anatomy 0.000 claims description 2
- 235000013601 eggs Nutrition 0.000 claims description 2
- 210000003999 epithelial cell of bile duct Anatomy 0.000 claims description 2
- 210000003743 erythrocyte Anatomy 0.000 claims description 2
- 210000002950 fibroblast Anatomy 0.000 claims description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 2
- 210000000265 leukocyte Anatomy 0.000 claims description 2
- 210000004698 lymphocyte Anatomy 0.000 claims description 2
- 210000000663 muscle cell Anatomy 0.000 claims description 2
- 210000001178 neural stem cell Anatomy 0.000 claims description 2
- 210000002569 neuron Anatomy 0.000 claims description 2
- 210000004409 osteocyte Anatomy 0.000 claims description 2
- 210000005259 peripheral blood Anatomy 0.000 claims description 2
- 239000011886 peripheral blood Substances 0.000 claims description 2
- 230000037361 pathway Effects 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 3
- 238000002347 injection Methods 0.000 description 40
- 239000007924 injection Substances 0.000 description 40
- 108010054624 red fluorescent protein Proteins 0.000 description 29
- 230000008569 process Effects 0.000 description 27
- 239000011521 glass Substances 0.000 description 24
- 239000002090 nanochannel Substances 0.000 description 16
- 238000004520 electroporation Methods 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- 239000006285 cell suspension Substances 0.000 description 10
- 238000005530 etching Methods 0.000 description 9
- 238000012545 processing Methods 0.000 description 9
- 239000000725 suspension Substances 0.000 description 9
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 8
- 229920001296 polysiloxane Polymers 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- BQRGNLJZBFXNCZ-UHFFFAOYSA-N calcein am Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)=C(OC(C)=O)C=C1OC1=C2C=C(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(=O)C)C(OC(C)=O)=C1 BQRGNLJZBFXNCZ-UHFFFAOYSA-N 0.000 description 6
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 239000013598 vector Substances 0.000 description 5
- 230000005684 electric field Effects 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 238000002659 cell therapy Methods 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 239000004205 dimethyl polysiloxane Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 3
- -1 polydimethylsiloxane Polymers 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 230000001131 transforming effect Effects 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102100028072 Fibroblast growth factor 4 Human genes 0.000 description 2
- 108090000381 Fibroblast growth factor 4 Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002073 fluorescence micrograph Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 210000002826 placenta Anatomy 0.000 description 2
- 239000002952 polymeric resin Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 229920003002 synthetic resin Polymers 0.000 description 2
- 230000007723 transport mechanism Effects 0.000 description 2
- FOXXZZGDIAQPQI-XKNYDFJKSA-N Asp-Pro-Ser-Ser Chemical compound OC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O FOXXZZGDIAQPQI-XKNYDFJKSA-N 0.000 description 1
- 101100239628 Danio rerio myca gene Proteins 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108700021430 Kruppel-Like Factor 4 Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 description 1
- 101150086694 SLC22A3 gene Proteins 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000002716 delivery method Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000010292 electrical insulation Methods 0.000 description 1
- 210000003722 extracellular fluid Anatomy 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000005660 hydrophilic surface Effects 0.000 description 1
- 230000005661 hydrophobic surface Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 108091082847 miR-7904 stem-loop Proteins 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 238000005309 stochastic process Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0609—Oocytes, oogonia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/061—Sperm cells, spermatogonia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Sustainable Development (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Electromagnetism (AREA)
- Mechanical Engineering (AREA)
- Developmental Biology & Embryology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicinal Chemistry (AREA)
- Reproductive Health (AREA)
- Analytical Chemistry (AREA)
- Gynecology & Obstetrics (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Transplantation (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Botany (AREA)
- General Physics & Mathematics (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15202518.5A EP3037517A1 (en) | 2014-12-28 | 2015-12-23 | Process for modifying a cell by putting material into the cell |
| JP2017535711A JP6805152B2 (ja) | 2014-12-28 | 2015-12-24 | 細胞に物質を注入して細胞を変換させる方法｛Ｍｅｔｈｏｄ ｆｏｒ Ｍｏｄｉｆｙｉｎｇ ａ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ｝ |
| PCT/KR2015/014293 WO2016108527A1 (en) | 2014-12-28 | 2015-12-24 | Process for modifying a cell by putting material into the cell |
| US14/980,713 US20160272961A1 (en) | 2014-12-28 | 2015-12-28 | Process for modifying a cell by putting material into the cell |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20140191302 | 2014-12-28 | ||
| KR1020140191302 | 2014-12-28 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20160079660A KR20160079660A (ko) | 2016-07-06 |
| KR102428464B1 true KR102428464B1 (ko) | 2022-08-03 |
Family
ID=56502546
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020150178183A Active KR102428464B1 (ko) | 2014-12-28 | 2015-12-14 | 세포에 물질을 주입하여 세포를 변환시키는 방법 |
| KR1020150178234A Ceased KR20160079661A (ko) | 2014-12-28 | 2015-12-14 | 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포 |
| KR1020150178130A Active KR102428463B1 (ko) | 2014-12-28 | 2015-12-14 | 세포에 물질을 주입하는 장치 및 제조 방법 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020150178234A Ceased KR20160079661A (ko) | 2014-12-28 | 2015-12-14 | 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포 |
| KR1020150178130A Active KR102428463B1 (ko) | 2014-12-28 | 2015-12-14 | 세포에 물질을 주입하는 장치 및 제조 방법 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20160272961A1 (enExample) |
| JP (3) | JP6883517B2 (enExample) |
| KR (3) | KR102428464B1 (enExample) |
| DK (1) | DK3037518T3 (enExample) |
| ES (1) | ES2808828T3 (enExample) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3037518B1 (en) | 2014-12-28 | 2020-05-06 | Femtobiomed Inc. | Device for putting material into cell |
| CN108251268A (zh) * | 2016-12-29 | 2018-07-06 | 上海新微技术研发中心有限公司 | 一种基因转染器及基因转染方法 |
| RS63202B1 (sr) | 2017-06-30 | 2022-06-30 | Inscripta Inc | Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| CN111372650A (zh) | 2017-09-30 | 2020-07-03 | 因思科瑞普特公司 | 流通式电穿孔仪器 |
| WO2019190874A1 (en) | 2018-03-29 | 2019-10-03 | Inscripta, Inc. | Automated control of cell growth rates for induction and transformation |
| WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| WO2019209926A1 (en) | 2018-04-24 | 2019-10-31 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| IL292273B2 (en) | 2018-08-14 | 2023-10-01 | Inscripta Inc | Devices, modules and methods for improved detection of edited sequences in living cells |
| US10752874B2 (en) | 2018-08-14 | 2020-08-25 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US11965154B2 (en) | 2018-08-30 | 2024-04-23 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| KR102129681B1 (ko) | 2018-10-22 | 2020-07-02 | 부경대학교 산학협력단 | 연속공정식 액적 전기천공 장치 및 이를 이용한 연속공정식 액적 전기천공 방법 |
| WO2020101254A1 (ko) * | 2018-11-12 | 2020-05-22 | 주식회사 펨토바이오메드 | 세포 내 물질 전달을 제어하는 방법 및 장치 |
| US12473522B2 (en) | 2019-06-12 | 2025-11-18 | Cellfe, Inc. | Methods and systems for cell labeling and imaging |
| US10907125B2 (en) | 2019-06-20 | 2021-02-02 | Inscripta, Inc. | Flow through electroporation modules and instrumentation |
| US10920189B2 (en) | 2019-06-21 | 2021-02-16 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| KR20220133257A (ko) | 2020-01-27 | 2022-10-04 | 인스크립타 인코포레이티드 | 전기천공 모듈 및 기구 |
| CN115605572A (zh) * | 2020-03-10 | 2023-01-13 | 哈因斯科技株式会社（Jp） | 经由复合纳米管的细胞内物质导入和提取技术 |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| CA3201944A1 (en) | 2020-11-18 | 2022-05-27 | Cellfe, Inc. | Methods and systems for mechanoporation-based payload delivery into biological cells |
| US12227729B2 (en) | 2020-12-24 | 2025-02-18 | Cellfe, Inc. | Methods and systems for high-throughput cell processing |
| GB202206443D0 (en) * | 2022-05-03 | 2022-06-15 | Ttp Plc | Apparatus and method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002516678A (ja) | 1998-06-03 | 2002-06-11 | ジェネトロニクス、インコーポレーテッド | 生体外遺伝子治療のためのフロースルー電気穿孔システム |
| JP2005510236A (ja) | 2001-11-27 | 2005-04-21 | セレクトリコン アーベー | 並列薬剤送達と細胞構造の電気穿孔法を併合した方法及びその使用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08322548A (ja) * | 1995-05-31 | 1996-12-10 | Nikon Corp | 細胞操作方法および細胞操作装置 |
| SE0003841D0 (sv) * | 2000-10-20 | 2000-10-20 | Daniel Chiu | A method and apparatus for penetration of lipid bilayer membranes |
| US20070087436A1 (en) * | 2003-04-11 | 2007-04-19 | Atsushi Miyawaki | Microinjection method and device |
| US7338796B1 (en) * | 2003-08-13 | 2008-03-04 | Sandia Corporation | Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species |
| US20050170510A1 (en) * | 2003-12-08 | 2005-08-04 | Yong Huang | Device and method for controlled electroporation and molecular delivery into cells and tissue |
| JP2005278480A (ja) * | 2004-03-29 | 2005-10-13 | Fujitsu Ltd | 物質導入装置及び物質導入用チップ |
| EP1941537A4 (en) * | 2005-10-20 | 2010-03-31 | Univ Ohio State | MEDICAMENT AND GENERIC RELEASE OF A POLYMER NANODIUM AND A NANOSTOP CELL PLASTER |
| JP4713671B1 (ja) * | 2010-01-22 | 2011-06-29 | ネッパジーン株式会社 | エレクトロポレーション法による外来遺伝子導入法 |
| JP5585204B2 (ja) * | 2010-05-18 | 2014-09-10 | 大日本印刷株式会社 | マイクロチップ及びその製造方法 |
| US9816086B2 (en) * | 2010-07-06 | 2017-11-14 | The Ohio State University | Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation |
| CN101928666B (zh) * | 2010-07-30 | 2013-04-03 | 北京大学 | 一种流式电穿孔装置及系统 |
| EP2768942B1 (en) * | 2011-10-17 | 2019-12-04 | Massachusetts Institute of Technology | Intracellular delivery |
-
2015
- 2015-12-14 KR KR1020150178183A patent/KR102428464B1/ko active Active
- 2015-12-14 KR KR1020150178234A patent/KR20160079661A/ko not_active Ceased
- 2015-12-14 KR KR1020150178130A patent/KR102428463B1/ko active Active
- 2015-12-23 ES ES15202528T patent/ES2808828T3/es active Active
- 2015-12-23 DK DK15202528.4T patent/DK3037518T3/da active
- 2015-12-24 JP JP2017535710A patent/JP6883517B2/ja active Active
- 2015-12-24 JP JP2017535708A patent/JP2018504110A/ja active Pending
- 2015-12-24 JP JP2017535711A patent/JP6805152B2/ja active Active
- 2015-12-28 US US14/980,713 patent/US20160272961A1/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002516678A (ja) | 1998-06-03 | 2002-06-11 | ジェネトロニクス、インコーポレーテッド | 生体外遺伝子治療のためのフロースルー電気穿孔システム |
| JP2005510236A (ja) | 2001-11-27 | 2005-04-21 | セレクトリコン アーベー | 並列薬剤送達と細胞構造の電気穿孔法を併合した方法及びその使用 |
Non-Patent Citations (2)
| Title |
|---|
| Choudhury. Continuous flow single cell electroporation in an ultrafast laser inscribed optofluidic device. 2012 International conference of fiber optics and photonics. 2013.6.25. 1부.* |
| Geng. Microfluidic electroporation for cellular analysis and delivery. Lab on a Chip. 2013, 13, 3803-3821 1부.* |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20160079659A (ko) | 2016-07-06 |
| KR20160079660A (ko) | 2016-07-06 |
| KR102428463B1 (ko) | 2022-08-03 |
| JP6883517B2 (ja) | 2021-06-09 |
| JP2018500044A (ja) | 2018-01-11 |
| DK3037518T3 (da) | 2020-07-13 |
| JP2018500045A (ja) | 2018-01-11 |
| JP2018504110A (ja) | 2018-02-15 |
| US20160272961A1 (en) | 2016-09-22 |
| ES2808828T3 (es) | 2021-03-02 |
| JP6805152B2 (ja) | 2020-12-23 |
| KR20160079661A (ko) | 2016-07-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102428464B1 (ko) | 세포에 물질을 주입하여 세포를 변환시키는 방법 | |
| US10100300B2 (en) | Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation | |
| Xie et al. | Nanostraw–electroporation system for highly efficient intracellular delivery and transfection | |
| Geng et al. | Flow-through electroporation based on constant voltage for large-volume transfection of cells | |
| Dong et al. | On-chip multiplexed single-cell patterning and controllable intracellular delivery | |
| US20250303415A1 (en) | Microfluidic devices and methods for high throughput electroporation | |
| Wu et al. | Micromotor-based localized electroporation and gene transfection of mammalian cells | |
| KR20140116374A (ko) | 세포 내 전달 | |
| Qu et al. | Surface-mediated intracellular delivery by physical membrane disruption | |
| Kumar | Microfluidic devices in nanotechnology: applications | |
| Kaladharan et al. | Microfluidic based physical approaches towards single-cell intracellular delivery and analysis | |
| US11999966B2 (en) | Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells | |
| US20160186212A1 (en) | Modified cell by putting material into the cell without using delivery vehicle | |
| US20080248575A1 (en) | Drug and Gene Delivery by Polymer Nanonozzle and Nanotip Cell Patch | |
| EP3037518B1 (en) | Device for putting material into cell | |
| Chen et al. | Flow‐Through Electroporation of HL‐60 White Blood Cell Suspensions using Nanoporous Membrane Electrodes | |
| EP3037517A1 (en) | Process for modifying a cell by putting material into the cell | |
| Kulbacka et al. | Ultrathin glass fiber microprobe for electroporation of arbitrary selected cell groups | |
| Brooks et al. | Microfluidic device for localized electroporation | |
| Chang | 3D Nanochannel Array Platform for High-throughput Cell Manipulation and Nano-electroporation | |
| Zhao | Micro/Nanofluidics Based Electroporation for Precise Material Delivery to Cells | |
| Hogquist | Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates | |
| Jung | Design, fabrication and characterization of micro/nano electroporation devices for drug/gene delivery | |
| HK40059870B (zh) | 细胞内传递 | |
| Fei | Membrane Sandwich Electroporation for In Vitro Gene Delivery |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 20151214 |
|
| PG1501 | Laying open of application | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20201123 Comment text: Request for Examination of Application Patent event code: PA02011R01I Patent event date: 20151214 Comment text: Patent Application |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20220216 Patent event code: PE09021S01D |
|
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 20220713 |
|
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 20220728 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 20220729 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration |