KR102428464B1 - 세포에 물질을 주입하여 세포를 변환시키는 방법 - Google Patents

세포에 물질을 주입하여 세포를 변환시키는 방법 Download PDF

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KR102428464B1
KR102428464B1 KR1020150178183A KR20150178183A KR102428464B1 KR 102428464 B1 KR102428464 B1 KR 102428464B1 KR 1020150178183 A KR1020150178183 A KR 1020150178183A KR 20150178183 A KR20150178183 A KR 20150178183A KR 102428464 B1 KR102428464 B1 KR 102428464B1
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cells
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stem cells
electrode
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KR20160079660A (ko
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이상현
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주식회사 펨토바이오메드
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Priority to EP15202518.5A priority Critical patent/EP3037517A1/en
Priority to JP2017535711A priority patent/JP6805152B2/ja
Priority to PCT/KR2015/014293 priority patent/WO2016108527A1/en
Priority to US14/980,713 priority patent/US20160272961A1/en
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
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    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

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  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
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KR1020150178183A 2014-12-28 2015-12-14 세포에 물질을 주입하여 세포를 변환시키는 방법 Active KR102428464B1 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP15202518.5A EP3037517A1 (en) 2014-12-28 2015-12-23 Process for modifying a cell by putting material into the cell
JP2017535711A JP6805152B2 (ja) 2014-12-28 2015-12-24 細胞に物質を注入して細胞を変換させる方法｛Ｍｅｔｈｏｄ ｆｏｒ Ｍｏｄｉｆｙｉｎｇ ａ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ｝
PCT/KR2015/014293 WO2016108527A1 (en) 2014-12-28 2015-12-24 Process for modifying a cell by putting material into the cell
US14/980,713 US20160272961A1 (en) 2014-12-28 2015-12-28 Process for modifying a cell by putting material into the cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20140191302 2014-12-28
KR1020140191302 2014-12-28

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KR20160079660A KR20160079660A (ko) 2016-07-06
KR102428464B1 true KR102428464B1 (ko) 2022-08-03

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KR1020150178183A Active KR102428464B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하여 세포를 변환시키는 방법
KR1020150178130A Active KR102428463B1 (ko) 2014-12-28 2015-12-14 세포에 물질을 주입하는 장치 및 제조 방법
KR1020150178234A Ceased KR20160079661A (ko) 2014-12-28 2015-12-14 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포

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KR1020150178234A Ceased KR20160079661A (ko) 2014-12-28 2015-12-14 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포

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US (1) US20160272961A1 (cg-RX-API-DMAC10.html)
JP (3) JP2018504110A (cg-RX-API-DMAC10.html)
KR (3) KR102428464B1 (cg-RX-API-DMAC10.html)
DK (1) DK3037518T3 (cg-RX-API-DMAC10.html)
ES (1) ES2808828T3 (cg-RX-API-DMAC10.html)

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MX2019015505A (es) 2017-06-30 2020-07-28 Inscripta Inc Metodos, modulos, instrumentos y sistemas de procesamiento de celulas automatizados.
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
US10435713B2 (en) 2017-09-30 2019-10-08 Inscripta, Inc. Flow through electroporation instrumentation
US10443031B1 (en) 2018-03-29 2019-10-15 Inscripta, Inc. Methods for controlling the growth of prokaryotic and eukaryotic cells
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US10508273B2 (en) 2018-04-24 2019-12-17 Inscripta, Inc. Methods for identifying selective binding pairs
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
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KR102129681B1 (ko) * 2018-10-22 2020-07-02 부경대학교 산학협력단 연속공정식 액적 전기천공 장치 및 이를 이용한 연속공정식 액적 전기천공 방법
CN112840015A (zh) * 2018-11-12 2021-05-25 飞秒生物医学有限公司 控制细胞内递送物质的方法及装置
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CN114008070A (zh) 2019-06-21 2022-02-01 因思科瑞普特公司 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
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KR20160079659A (ko) 2016-07-06
DK3037518T3 (da) 2020-07-13
JP6883517B2 (ja) 2021-06-09
JP2018500044A (ja) 2018-01-11
JP2018500045A (ja) 2018-01-11
KR20160079661A (ko) 2016-07-06
US20160272961A1 (en) 2016-09-22
ES2808828T3 (es) 2021-03-02
JP2018504110A (ja) 2018-02-15
KR102428463B1 (ko) 2022-08-03
KR20160079660A (ko) 2016-07-06
JP6805152B2 (ja) 2020-12-23

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