JP2018504110A - 運搬体の使用なしで細胞に物質を注入して変換させた細胞（Ｍｏｄｉｆｉｅｄ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ ｗｉｔｈｏｕｔ Ｕｓｉｎｇ Ｖｅｈｉｃｌｅ）［発明の詳細な説明］ - Google Patents

運搬体の使用なしで細胞に物質を注入して変換させた細胞（Ｍｏｄｉｆｉｅｄ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ ｗｉｔｈｏｕｔ Ｕｓｉｎｇ Ｖｅｈｉｃｌｅ）［発明の詳細な説明］ Download PDF

Info

Publication number
JP2018504110A
JP2018504110A JP2017535708A JP2017535708A JP2018504110A JP 2018504110 A JP2018504110 A JP 2018504110A JP 2017535708 A JP2017535708 A JP 2017535708A JP 2017535708 A JP2017535708 A JP 2017535708A JP 2018504110 A JP2018504110 A JP 2018504110A
Authority
JP
Japan
Prior art keywords
cell
cells
passage
substance
stem cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017535708A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018504110A5 (ja
Inventor
サンギュン リー
サンギュン リー
Original Assignee
フェムトファブ カンパニー リミテッド
フェムトファブ カンパニー リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by フェムトファブ カンパニー リミテッド, フェムトファブ カンパニー リミテッド filed Critical フェムトファブ カンパニー リミテッド
Priority claimed from PCT/KR2015/014295 external-priority patent/WO2016108529A1/en
Publication of JP2018504110A publication Critical patent/JP2018504110A/ja
Publication of JP2018504110A5 publication Critical patent/JP2018504110A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Electromagnetism (AREA)
  • Mechanical Engineering (AREA)
  • Developmental Biology & Embryology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Reproductive Health (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Transplantation (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
JP2017535708A 2014-12-28 2015-12-24 運搬体の使用なしで細胞に物質を注入して変換させた細胞（Ｍｏｄｉｆｉｅｄ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ ｗｉｔｈｏｕｔ Ｕｓｉｎｇ Ｖｅｈｉｃｌｅ）［発明の詳細な説明］ Pending JP2018504110A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
KR20140191302 2014-12-28
KR10-2014-0191302 2014-12-28
KR10-2015-0178234 2015-12-14
KR1020150178234A KR20160079661A (ko) 2014-12-28 2015-12-14 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포
PCT/KR2015/014295 WO2016108529A1 (en) 2014-12-28 2015-12-24 Modified cell prepared by putting material into the cell without using delivery vehicle

Publications (2)

Publication Number Publication Date
JP2018504110A true JP2018504110A (ja) 2018-02-15
JP2018504110A5 JP2018504110A5 (ja) 2019-02-07

Family

ID=56502546

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2017535708A Pending JP2018504110A (ja) 2014-12-28 2015-12-24 運搬体の使用なしで細胞に物質を注入して変換させた細胞（Ｍｏｄｉｆｉｅｄ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ ｗｉｔｈｏｕｔ Ｕｓｉｎｇ Ｖｅｈｉｃｌｅ）［発明の詳細な説明］
JP2017535711A Active JP6805152B2 (ja) 2014-12-28 2015-12-24 細胞に物質を注入して細胞を変換させる方法｛Ｍｅｔｈｏｄ ｆｏｒ Ｍｏｄｉｆｙｉｎｇ ａ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ｝
JP2017535710A Active JP6883517B2 (ja) 2014-12-28 2015-12-24 細胞に物質を注入する装置および製造方法（Ｄｅｖｉｃｅ ｆｏｒ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ Ｃｅｌｌ）

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2017535711A Active JP6805152B2 (ja) 2014-12-28 2015-12-24 細胞に物質を注入して細胞を変換させる方法｛Ｍｅｔｈｏｄ ｆｏｒ Ｍｏｄｉｆｙｉｎｇ ａ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ｝
JP2017535710A Active JP6883517B2 (ja) 2014-12-28 2015-12-24 細胞に物質を注入する装置および製造方法（Ｄｅｖｉｃｅ ｆｏｒ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ Ｃｅｌｌ）

Country Status (5)

Country Link
US (1) US20160272961A1 (cg-RX-API-DMAC10.html)
JP (3) JP2018504110A (cg-RX-API-DMAC10.html)
KR (3) KR102428464B1 (cg-RX-API-DMAC10.html)
DK (1) DK3037518T3 (cg-RX-API-DMAC10.html)
ES (1) ES2808828T3 (cg-RX-API-DMAC10.html)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3037518B1 (en) 2014-12-28 2020-05-06 Femtobiomed Inc. Device for putting material into cell
CN108251268A (zh) * 2016-12-29 2018-07-06 上海新微技术研发中心有限公司 一种基因转染器及基因转染方法
MX2019015505A (es) 2017-06-30 2020-07-28 Inscripta Inc Metodos, modulos, instrumentos y sistemas de procesamiento de celulas automatizados.
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
US10435713B2 (en) 2017-09-30 2019-10-08 Inscripta, Inc. Flow through electroporation instrumentation
US10443031B1 (en) 2018-03-29 2019-10-15 Inscripta, Inc. Methods for controlling the growth of prokaryotic and eukaryotic cells
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10508273B2 (en) 2018-04-24 2019-12-17 Inscripta, Inc. Methods for identifying selective binding pairs
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
IL292273B2 (en) 2018-08-14 2023-10-01 Inscripta Inc Devices, modules and methods for improved detection of edited sequences in living cells
CA3108892A1 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
KR102129681B1 (ko) * 2018-10-22 2020-07-02 부경대학교 산학협력단 연속공정식 액적 전기천공 장치 및 이를 이용한 연속공정식 액적 전기천공 방법
CN112840015A (zh) * 2018-11-12 2021-05-25 飞秒生物医学有限公司 控制细胞内递送物质的方法及装置
WO2020252215A1 (en) 2019-06-12 2020-12-17 Cellfe, Inc. Methods and systems for cell labeling and imaging
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
CN114008070A (zh) 2019-06-21 2022-02-01 因思科瑞普特公司 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
CN115135370A (zh) 2020-01-27 2022-09-30 因思科瑞普特公司 电穿孔模块和仪器
JP7824650B2 (ja) * 2020-03-10 2026-03-05 ハインツテック株式会社 複合ナノチューブを介した細胞内物質導入および抽出技術
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
IL302976A (en) 2020-11-18 2023-07-01 Cellfe Inc Mechanoporation-based methods and systems for high-throughput charge transfer into biological cells
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
GB202206443D0 (en) * 2022-05-03 2022-06-15 Ttp Plc Apparatus and method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140256047A1 (en) * 2010-07-06 2014-09-11 The Ohio State University Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation
JP2014533936A (ja) * 2011-10-17 2014-12-18 マサチューセッツ インスティテュート オブ テクノロジー 細胞内送達

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08322548A (ja) * 1995-05-31 1996-12-10 Nikon Corp 細胞操作方法および細胞操作装置
US6027488A (en) 1998-06-03 2000-02-22 Genetronics, Inc. Flow-through electroporation system for ex vivo gene therapy
SE0003841D0 (sv) * 2000-10-20 2000-10-20 Daniel Chiu A method and apparatus for penetration of lipid bilayer membranes
AU2002353741A1 (en) * 2001-11-27 2003-06-10 Cellectricon Ab A method for combined sequential agent delivery and electroporation for cell structures and use thereof
US20070087436A1 (en) * 2003-04-11 2007-04-19 Atsushi Miyawaki Microinjection method and device
US7338796B1 (en) * 2003-08-13 2008-03-04 Sandia Corporation Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species
WO2005056788A1 (en) * 2003-12-08 2005-06-23 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery into cells and tissue
JP2005278480A (ja) * 2004-03-29 2005-10-13 Fujitsu Ltd 物質導入装置及び物質導入用チップ
WO2007053802A2 (en) * 2005-10-20 2007-05-10 The Ohio State University Drug and gene delivery by polymer nanonozzle and nanotip cell patch
JP4713671B1 (ja) * 2010-01-22 2011-06-29 ネッパジーン株式会社 エレクトロポレーション法による外来遺伝子導入法
JP5585204B2 (ja) * 2010-05-18 2014-09-10 大日本印刷株式会社 マイクロチップ及びその製造方法
CN101928666B (zh) * 2010-07-30 2013-04-03 北京大学 一种流式电穿孔装置及系统

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140256047A1 (en) * 2010-07-06 2014-09-11 The Ohio State University Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation
JP2014533936A (ja) * 2011-10-17 2014-12-18 マサチューセッツ インスティテュート オブ テクノロジー 細胞内送達

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOUKANY, P.E. ET AL.: "Nanochannel electroporation delivers precise amounts of biomolecules into living cells", NATURE NANOTECHNOLOGY, vol. 6, JPN6019036963, 2011, pages 747 - 753, ISSN: 0004282908 *
XIE, X. ET AL.: "Nanostraw-Electroporation System for Highly Efficient Intracellular Delivery and Transfection", ACS NANO, vol. 7, no. 5, JPN6019036965, 2013, pages 4351 - 4358, ISSN: 0004147655 *

Also Published As

Publication number Publication date
KR20160079659A (ko) 2016-07-06
DK3037518T3 (da) 2020-07-13
JP6883517B2 (ja) 2021-06-09
JP2018500044A (ja) 2018-01-11
JP2018500045A (ja) 2018-01-11
KR20160079661A (ko) 2016-07-06
US20160272961A1 (en) 2016-09-22
ES2808828T3 (es) 2021-03-02
KR102428463B1 (ko) 2022-08-03
KR20160079660A (ko) 2016-07-06
KR102428464B1 (ko) 2022-08-03
JP6805152B2 (ja) 2020-12-23

Similar Documents

Publication Publication Date Title
JP6805152B2 (ja) 細胞に物質を注入して細胞を変換させる方法｛Ｍｅｔｈｏｄ ｆｏｒ Ｍｏｄｉｆｙｉｎｇ ａ Ｃｅｌｌ ｂｙ Ｐｕｔｔｉｎｇ Ｍａｔｅｒｉａｌ ｉｎｔｏ ｔｈｅ Ｃｅｌｌ｝
Morshedi Rad et al. A comprehensive review on intracellular delivery
Rad et al. A comprehensive review on intracellular delivery
Tian et al. Micro/nanosystems for controllable drug delivery to the brain
Chang et al. Micro-/nanoscale electroporation
Xie et al. Nanostraw–electroporation system for highly efficient intracellular delivery and transfection
Qu et al. Surface-mediated intracellular delivery by physical membrane disruption
US20180346873A1 (en) Artificial micro-gland
US20250303415A1 (en) Microfluidic devices and methods for high throughput electroporation
Yang et al. Micro-/nano-electroporation for active gene delivery
Zhou et al. Biofabrication strategies with single-cell resolution: a review
Hu et al. Automating CAR‐T transfection with micro and nano‐technologies
Choi et al. Magnetic-based tissue engineering: principles, applications, and future prospects in biofabrication
Han et al. A low-cost smartphone controlled portable system with accurately confined on-chip 3D electrodes for flow-through cell electroporation
Truong et al. Strategies to control cellular spatial organization in microphysiological systems
Tang et al. Ultrahigh efficiency and minimalist intracellular delivery of macromolecules mediated by latent-photothermal surfaces
US20160186212A1 (en) Modified cell by putting material into the cell without using delivery vehicle
Chen et al. Flow‐Through Electroporation of HL‐60 White Blood Cell Suspensions using Nanoporous Membrane Electrodes
US20190032000A1 (en) Device for putting material into cell
EP3037517A1 (en) Process for modifying a cell by putting material into the cell
Li et al. Bipolar Electrode-Based Precise Manipulation and Selective Electroporation of Cells
Jayasinghe Cell engineering: spearheading the next generation in healthcare
Rad Developing Microfluidic Tools for Intracellular Delivery
Chang et al. Nonviral Transfection Methods of Efficient Gene Delivery: Micro-/Nano-Technology for Electroporation
Kalyan EXPANDING VORTEX TRAPPING AND ELECTROPORATION CAPABILITIES TO SMALL CELLS

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20181220

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20181220

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191112

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20200623