ES2808828T3 - Dispositivo para colocar material en célula - Google Patents
Dispositivo para colocar material en célula Download PDFInfo
- Publication number
- ES2808828T3 ES2808828T3 ES15202528T ES15202528T ES2808828T3 ES 2808828 T3 ES2808828 T3 ES 2808828T3 ES 15202528 T ES15202528 T ES 15202528T ES 15202528 T ES15202528 T ES 15202528T ES 2808828 T3 ES2808828 T3 ES 2808828T3
- Authority
- ES
- Spain
- Prior art keywords
- cell
- pass
- passage
- potential difference
- solid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
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- 230000008569 process Effects 0.000 claims description 43
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- 229920001169 thermoplastic Polymers 0.000 claims description 6
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- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 9
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 8
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
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- 239000004642 Polyimide Substances 0.000 description 2
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- 238000011084 recovery Methods 0.000 description 2
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- FOXXZZGDIAQPQI-XKNYDFJKSA-N Asp-Pro-Ser-Ser Chemical compound OC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O FOXXZZGDIAQPQI-XKNYDFJKSA-N 0.000 description 1
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- 101150086694 SLC22A3 gene Proteins 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
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- 238000010292 electrical insulation Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Sustainable Development (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Electromagnetism (AREA)
- Mechanical Engineering (AREA)
- Developmental Biology & Embryology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Reproductive Health (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Gynecology & Obstetrics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Dispersion Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Transplantation (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pathology (AREA)
- Immunology (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20140191302 | 2014-12-28 | ||
| KR1020150178130A KR102428463B1 (ko) | 2014-12-28 | 2015-12-14 | 세포에 물질을 주입하는 장치 및 제조 방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2808828T3 true ES2808828T3 (es) | 2021-03-02 |
Family
ID=56502546
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES15202528T Active ES2808828T3 (es) | 2014-12-28 | 2015-12-23 | Dispositivo para colocar material en célula |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20160272961A1 (cg-RX-API-DMAC10.html) |
| JP (3) | JP2018504110A (cg-RX-API-DMAC10.html) |
| KR (3) | KR102428464B1 (cg-RX-API-DMAC10.html) |
| DK (1) | DK3037518T3 (cg-RX-API-DMAC10.html) |
| ES (1) | ES2808828T3 (cg-RX-API-DMAC10.html) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3037518B1 (en) | 2014-12-28 | 2020-05-06 | Femtobiomed Inc. | Device for putting material into cell |
| CN108251268A (zh) * | 2016-12-29 | 2018-07-06 | 上海新微技术研发中心有限公司 | 一种基因转染器及基因转染方法 |
| MX2019015505A (es) | 2017-06-30 | 2020-07-28 | Inscripta Inc | Metodos, modulos, instrumentos y sistemas de procesamiento de celulas automatizados. |
| US10738327B2 (en) | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| US10435713B2 (en) | 2017-09-30 | 2019-10-08 | Inscripta, Inc. | Flow through electroporation instrumentation |
| US10443031B1 (en) | 2018-03-29 | 2019-10-15 | Inscripta, Inc. | Methods for controlling the growth of prokaryotic and eukaryotic cells |
| WO2019200004A1 (en) | 2018-04-13 | 2019-10-17 | Inscripta, Inc. | Automated cell processing instruments comprising reagent cartridges |
| US10508273B2 (en) | 2018-04-24 | 2019-12-17 | Inscripta, Inc. | Methods for identifying selective binding pairs |
| US10557216B2 (en) | 2018-04-24 | 2020-02-11 | Inscripta, Inc. | Automated instrumentation for production of T-cell receptor peptide libraries |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| US10752874B2 (en) | 2018-08-14 | 2020-08-25 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| US10532324B1 (en) | 2018-08-14 | 2020-01-14 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| IL292273B2 (en) | 2018-08-14 | 2023-10-01 | Inscripta Inc | Devices, modules and methods for improved detection of edited sequences in living cells |
| CA3108892A1 (en) | 2018-08-30 | 2020-04-23 | Inscripta, Inc. | Improved detection of nuclease edited sequences in automated modules and instruments |
| KR102129681B1 (ko) * | 2018-10-22 | 2020-07-02 | 부경대학교 산학협력단 | 연속공정식 액적 전기천공 장치 및 이를 이용한 연속공정식 액적 전기천공 방법 |
| CN112840015A (zh) * | 2018-11-12 | 2021-05-25 | 飞秒生物医学有限公司 | 控制细胞内递送物质的方法及装置 |
| WO2020252215A1 (en) | 2019-06-12 | 2020-12-17 | Cellfe, Inc. | Methods and systems for cell labeling and imaging |
| US10907125B2 (en) | 2019-06-20 | 2021-02-02 | Inscripta, Inc. | Flow through electroporation modules and instrumentation |
| CN114008070A (zh) | 2019-06-21 | 2022-02-01 | 因思科瑞普特公司 | 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变 |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US10689669B1 (en) | 2020-01-11 | 2020-06-23 | Inscripta, Inc. | Automated multi-module cell processing methods, instruments, and systems |
| CN115135370A (zh) | 2020-01-27 | 2022-09-30 | 因思科瑞普特公司 | 电穿孔模块和仪器 |
| JP7824650B2 (ja) * | 2020-03-10 | 2026-03-05 | ハインツテック株式会社 | 複合ナノチューブを介した細胞内物質導入および抽出技術 |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| IL302976A (en) | 2020-11-18 | 2023-07-01 | Cellfe Inc | Mechanoporation-based methods and systems for high-throughput charge transfer into biological cells |
| US12227729B2 (en) | 2020-12-24 | 2025-02-18 | Cellfe, Inc. | Methods and systems for high-throughput cell processing |
| GB202206443D0 (en) * | 2022-05-03 | 2022-06-15 | Ttp Plc | Apparatus and method |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08322548A (ja) * | 1995-05-31 | 1996-12-10 | Nikon Corp | 細胞操作方法および細胞操作装置 |
| US6027488A (en) | 1998-06-03 | 2000-02-22 | Genetronics, Inc. | Flow-through electroporation system for ex vivo gene therapy |
| SE0003841D0 (sv) * | 2000-10-20 | 2000-10-20 | Daniel Chiu | A method and apparatus for penetration of lipid bilayer membranes |
| AU2002353741A1 (en) * | 2001-11-27 | 2003-06-10 | Cellectricon Ab | A method for combined sequential agent delivery and electroporation for cell structures and use thereof |
| US20070087436A1 (en) * | 2003-04-11 | 2007-04-19 | Atsushi Miyawaki | Microinjection method and device |
| US7338796B1 (en) * | 2003-08-13 | 2008-03-04 | Sandia Corporation | Vesicle-based method and apparatus for collecting, manipulating, and chemically processing trace macromolecular species |
| WO2005056788A1 (en) * | 2003-12-08 | 2005-06-23 | Excellin Life Sciences, Inc. | Device and method for controlled electroporation and molecular delivery into cells and tissue |
| JP2005278480A (ja) * | 2004-03-29 | 2005-10-13 | Fujitsu Ltd | 物質導入装置及び物質導入用チップ |
| WO2007053802A2 (en) * | 2005-10-20 | 2007-05-10 | The Ohio State University | Drug and gene delivery by polymer nanonozzle and nanotip cell patch |
| JP4713671B1 (ja) * | 2010-01-22 | 2011-06-29 | ネッパジーン株式会社 | エレクトロポレーション法による外来遺伝子導入法 |
| JP5585204B2 (ja) * | 2010-05-18 | 2014-09-10 | 大日本印刷株式会社 | マイクロチップ及びその製造方法 |
| US9816086B2 (en) * | 2010-07-06 | 2017-11-14 | The Ohio State University | Dose and location controlled drug/gene/particle delivery to individual cells by nanoelectroporation |
| CN101928666B (zh) * | 2010-07-30 | 2013-04-03 | 北京大学 | 一种流式电穿孔装置及系统 |
| WO2013059343A1 (en) * | 2011-10-17 | 2013-04-25 | Massachusetts Institute Of Technology | Intracellular delivery |
-
2015
- 2015-12-14 KR KR1020150178183A patent/KR102428464B1/ko active Active
- 2015-12-14 KR KR1020150178130A patent/KR102428463B1/ko active Active
- 2015-12-14 KR KR1020150178234A patent/KR20160079661A/ko not_active Ceased
- 2015-12-23 DK DK15202528.4T patent/DK3037518T3/da active
- 2015-12-23 ES ES15202528T patent/ES2808828T3/es active Active
- 2015-12-24 JP JP2017535708A patent/JP2018504110A/ja active Pending
- 2015-12-24 JP JP2017535711A patent/JP6805152B2/ja active Active
- 2015-12-24 JP JP2017535710A patent/JP6883517B2/ja active Active
- 2015-12-28 US US14/980,713 patent/US20160272961A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| KR20160079659A (ko) | 2016-07-06 |
| DK3037518T3 (da) | 2020-07-13 |
| JP6883517B2 (ja) | 2021-06-09 |
| JP2018500044A (ja) | 2018-01-11 |
| JP2018500045A (ja) | 2018-01-11 |
| KR20160079661A (ko) | 2016-07-06 |
| US20160272961A1 (en) | 2016-09-22 |
| JP2018504110A (ja) | 2018-02-15 |
| KR102428463B1 (ko) | 2022-08-03 |
| KR20160079660A (ko) | 2016-07-06 |
| KR102428464B1 (ko) | 2022-08-03 |
| JP6805152B2 (ja) | 2020-12-23 |
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| Watanabe | Laser nanosurgery, manipulation, and transportation of cells and tissues | |
| Gupta | DNA Translocation and Cell Electroporation in Micro and Nanofluidic Devices | |
| Jung | Design, fabrication and characterization of micro/nano electroporation devices for drug/gene delivery | |
| Paterson et al. | Violet diode-assisted photoporation and transfection of cells | |
| Butt | The microfluidic intravital window: a tool for manipulation and imaging of the tumor microenvironment |