KR102411104B1 - Method for producing ginseng concentrate using plate evaporation concentration technology - Google Patents

Method for producing ginseng concentrate using plate evaporation concentration technology Download PDF

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KR102411104B1
KR102411104B1 KR1020220017913A KR20220017913A KR102411104B1 KR 102411104 B1 KR102411104 B1 KR 102411104B1 KR 1020220017913 A KR1020220017913 A KR 1020220017913A KR 20220017913 A KR20220017913 A KR 20220017913A KR 102411104 B1 KR102411104 B1 KR 102411104B1
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ginseng
extract
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최성근
장성수
이창순
전병선
이건희
박기태
김혜원
김다영
공병만
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대동고려삼 주식회사
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    • A23V2250/2124Ginseng
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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    • A23V2300/50Concentrating, enriching or enhancing in functional factors

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Abstract

본 발명은 (1) 인삼을 분쇄한 인삼 분쇄물에 주정을 첨가하여 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및 (2) 상기 (1)단계의 제조한 인삼 추출액을 판형증발농축기로 농축하는 단계를 포함하여 제조하는 것을 특징으로 하는 인삼 농축액의 제조방법 및 상기 방법으로 제조된 인삼 농축액에 관한것이다.The present invention comprises the steps of (1) extracting ginseng by adding alcohol to the pulverized ginseng product, followed by filtration to prepare a ginseng extract; and (2) concentrating the ginseng extract prepared in step (1) with a plate-type evaporator.

Description

판형증발농축기술을 이용한 인삼 농축액의 제조방법{Method for producing ginseng concentrate using plate evaporation concentration technology}Method for producing ginseng concentrate using plate evaporation concentration technology

본 발명은 인삼류를 추출한 인삼 추출액을 판형증발농축기로 농축하는 단계를 포함하여 제조하는 것을 특징으로 하는 인삼 농축액의 제조방법 및 상기 방법으로 제조된 인삼 농축액에 관한 것으로, 본 발명의 인삼 농축액은 유효성분 손실을 최소화하면서, 특정 진세노사이드 함량을 증진시키고 보다 효율적으로 농축할 수 있다.The present invention relates to a method for producing a ginseng concentrate, comprising the step of concentrating the ginseng extract obtained by extracting ginseng with a plate-type evaporator, and to a ginseng concentrate prepared by the method, wherein the ginseng concentrate of the present invention comprises an active ingredient It is possible to enhance the specific ginsenoside content and to concentrate more efficiently, while minimizing losses.

옛날부터 우리나라의 고려인삼은 세계적으로도 그 약효가 인정되어 한방에서 널리 사용되고 있다. 인삼은 우리나라의 대표적 생약제로서 생체를 방어하며, 독성물질에 대한 간장 상해방어, 당뇨병과 고지혈증 예방, 면역기능 증진 및 항암 기능 등과 같은 유용한 약리효능을 발휘하며, 특히 홍삼은 부작용이 거의 없는 생약으로 인정되고 있다.Since ancient times, Korean ginseng has been widely used in oriental medicine because its medicinal efficacy has been recognized worldwide. As a representative herbal medicine in Korea, ginseng defends the living body and exerts useful pharmacological effects such as protection against toxic substances in the liver, prevention of diabetes and hyperlipidemia, enhancement of immune function and anticancer function. is becoming

수확한 수삼은 약 75% 수분을 함유하여 보관이 어려워 주로 가공된 홍삼류가 이용된다. 수삼은 열처리를 기본으로 하는 증삼과정에서 함유된 당성분과 아미노산과의 카라멜화에 의한 적갈색을 나타내므로 홍삼이라고 한다.Harvested fresh ginseng contains about 75% water and is difficult to store, so processed red ginseng is mainly used. Fresh ginseng is called red ginseng because it shows reddish-brown color due to caramelization of amino acids and sugars contained in the steaming process based on heat treatment.

따라서 홍삼으로 사용하는 중요한 이유는 수삼과 백삼은 진세노사이드가 약 24종 함유되지만, 홍삼에서는 약 38종 이상으로 증가함은 물론 적은 함량으로도 우수한 생리활성 효능을 발휘하는 Rg2, Rg3, Rg5, Rg6, Rk1, Rk3, Rh1, Rh2, Rh4, F4 등 특정 진세노사이드(Ginsenoside) 성분이 새롭게 생성되고 증가한다는 점이다.Therefore, the important reason for using red ginseng is that fresh ginseng and white ginseng contain about 24 kinds of ginsenoside, but in red ginseng, it increases to about 38 kinds or more, and Rg2, Rg3, Rg5, Rg2, Rg3, Rg5, The point is that specific ginsenoside components such as Rg6, Rk1, Rk3, Rh1, Rh2, Rh4, and F4 are newly created and increased.

인삼과 홍삼에 함유된 사포닌은 당 부분과 비 당부분으로 구성되는 식물성 배당체로서, 특별히 인삼과 홍삼에 함유된 사포닌을 진세노사이드라 명명하고 있다. 이러한 진세노사이드류는 인삼 사포닌 배당체가 열 반응에 의하여 가수분해되어 생성되는 프로사포게닌(Prosapogenin) 형태의 생산물인데, 최근에는 다양한 물리 화학적인 수단을 적용하여 프로사포게닌 함량이 고농도로 함유되는 홍삼의 제조방법이 소개되고 있다.The saponins contained in ginseng and red ginseng are plant glycosides composed of a sugar part and a non-saccharide part. In particular, saponins contained in ginseng and red ginseng are called ginsenosides. These ginsenosides are products in the form of prosapogenin produced by hydrolysis of ginseng saponin glycosides by thermal reaction. A manufacturing method is introduced.

현재 많은 연구를 통하여 홍삼화 과정 중 새로운 진세노사이드가 생성되는 기전이 밝혀졌다. 홍삼의 사포닌은 타 식물계에 거의 존재하지 않는 다마렌(Dammarane) 계열의 트리테르페노이드 사포닌(Triterpenoid Saponin)으로서, 이들은 크게 프로토파낙사디올(Portopanaxadiol; PPD), 프로토파낙사트리올(Protopanaxatriol; PPT) 그룹으로 분류된다.Currently, many studies have revealed the mechanism by which new ginsenosides are generated during the process of red ginseng. The saponin of red ginseng is a triterpenoid saponin of the Dammarane series that hardly exists in other plant systems, and these are mainly protopanaxadiol (PPD), protopanaxatriol (PPT). ) are classified into groups.

다시 프로토파낙사디올계 진세노사이드는 C-3 및 C-20의 OH기에 당 모이어티(Sugar moiety(*당 분자, 당기, Sugar Chain, Glycosidic bond))를 갖는 구조이며, 프로토파낙사트리올계 진세노사이드는 C-3, C-6 및 C-20의 OH기에 당 모이어티가 결합된 구조이다. 이들 당 사슬(Sugar Chain) 중, C-20의 글리코시드 결합(Glycosidic bond)은 결합력이 비교적 약하므로 먼저 C-20의 글리코시드 결합이 가수분해되어 프로사포게닌이 얻어지며, 이어서 C-3 혹은 C-6 위치에서도 당 단위(Sugar unit)가 소실된다.Again, the protopanaxadiol-based ginsenoside has a structure having a sugar moiety (*sugar molecule, sugar chain, glycosidic bond) in the OH groups of C-3 and C-20, and protopanaxatriol-based Ginsenoside is a structure in which a sugar moiety is bonded to C-3, C-6 and C-20 OH groups. Among these sugar chains, the glycosidic bond of C-20 has relatively weak binding power, so first, the glycosidic bond of C-20 is hydrolyzed to obtain prosapogenin, and then C-3 or A sugar unit is also lost at the C-6 position.

인삼의 열 및 산 처리로 인한 효과에 의해서 Rg3, Rg5, Rg6, Rk1, Rs3, F4 등 특정 진세노사이드 성분이 증가하거나 새롭게 생성되는 장점은 있으나, 총 사포닌 함량이 감소하거나 탄 냄새가 증가하거나 또는 전체적인 공정에 많은 시간이 소비되는 문제점이 상존하고 있다.Specific ginsenoside components such as Rg3, Rg5, Rg6, Rk1, Rs3, and F4 are increased or newly generated by the effect of heat and acid treatment of ginseng, but the total saponin content decreases or the burnt smell increases, or There is a problem that a lot of time is consumed in the overall process.

한국등록특허 제1799256호에는 인삼 농축액의 제조방법이 개시되어 있고, 한국등록특허 제1123642호에는 인삼 특유의 향과 쓴맛을 감소시킨 인삼 농축액의 제조방법이 개시되어 있으나, 본 발명의 판형증발농축기술을 이용한 인삼 농축액의 제조방법과는 상이하다.Korean Patent No. 1799256 discloses a method for producing a ginseng concentrate, and Korean Patent No. 1123642 discloses a method for producing a ginseng concentrate with reduced flavor and bitterness unique to ginseng, but the plate-type evaporation concentration technology of the present invention It is different from the manufacturing method of ginseng concentrate using

본 발명은 상기와 같은 요구에 의해 안출된 것으로서, 본 발명의 목적은 인삼의 유효성분 손실을 최소화하면서 특정 진세노사이드 함량은 증진되고 보다 효율적으로 농축시킬 수 있는 인삼 농축액의 제조방법을 제공하는 데 있다.The present invention has been devised by the above needs, and an object of the present invention is to provide a method for producing a ginseng concentrate that can be concentrated more efficiently and a specific ginsenoside content is improved while minimizing the loss of active ingredients of ginseng. have.

상기 과제를 해결하기 위해, 본 발명은 (1) 인삼을 분쇄한 인삼 분쇄물에 주정을 첨가하여 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및 (2) 상기 (1)단계의 제조한 인삼 추출액을 판형증발농축기로 농축하는 단계를 포함하여 제조하는 것을 특징으로 하는 인삼 농축액의 제조방법을 제공한다.In order to solve the above problems, the present invention comprises the steps of (1) extracting by adding alcohol to the pulverized ginseng pulverized ginseng, followed by filtration to prepare a ginseng extract; and (2) concentrating the ginseng extract prepared in step (1) with a plate-type evaporator.

또한, 본 발명은 상기 방법으로 제조된 인삼 농축액을 제공한다.In addition, the present invention provides a ginseng concentrate prepared by the above method.

본 발명의 인삼류 농축액은 유효성분을 손실시키지 않고 보다 효율적으로 농축할 수 있고, 특정 진세노사이드 함량도 증진되어 품질이 우수한 이점이 있다. 또한, 인삼 고유의 쓴맛과 강한 향은 감소하여 누구나 쉽게 음용할 수 있게 되는 효과가 있다.The ginseng concentrate of the present invention can be concentrated more efficiently without loss of active ingredients, and the content of specific ginsenosides is also increased, so that the quality is excellent. In addition, the inherent bitter taste and strong flavor of ginseng are reduced, so that anyone can easily drink it.

도 1은 인삼 추출액을 농축하기 위한 감압농축기와 판형증발농축기의 원리를 비교한 개략도이다.
도 2는 본 발명의 인삼 추출액을 농축하기 위한 판형증발농축기와 연속식 열처리 과정을 보여주는 개략도이다.
도 3은 감압농축기와 판형증발농축기로 농축한 후 희석한 홍삼 희석액을 비교한 사진이다.
도 4는 연속식 열처리 시간에 따른 홍삼 농축액의 진세노사이드 함량을 비교한 그래프이다.
도 5는 연속식 열처리 시간에 따른 흑삼 농축액의 진세노사이드 함량을 비교한 그래프이다.
1 is a schematic diagram comparing the principles of a vacuum concentrator and a plate-type evaporator for concentrating a ginseng extract.
2 is a schematic diagram showing a plate-type evaporator and a continuous heat treatment process for concentrating the ginseng extract of the present invention.
3 is a photograph comparing the diluted red ginseng solution diluted after concentration with a reduced pressure concentrator and a plate-type evaporator.
4 is a graph comparing the ginsenoside content of the red ginseng concentrate according to the continuous heat treatment time.
5 is a graph comparing the ginsenoside content of the black ginseng concentrate according to the continuous heat treatment time.

본 발명의 목적을 달성하기 위하여, 본 발명은In order to achieve the object of the present invention, the present invention

(1) 인삼을 분쇄한 인삼 분쇄물에 주정을 첨가하여 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및(1) preparing a ginseng extract by adding alcohol to the pulverized ginseng pulverized ginseng, followed by extraction; and

(2) 상기 (1)단계의 제조한 인삼 추출액을 판형증발농축기로 농축하는 단계를 포함하여 제조하는 것을 특징으로 하는 인삼 농축액의 제조방법을 제공한다.(2) It provides a method for producing a ginseng concentrate, comprising the step of concentrating the ginseng extract prepared in step (1) with a plate-type evaporator.

본 발명의 인삼 농축액의 제조방법에서, 상기 인삼은 수삼, 홍삼, 건삼, 백삼, 흑삼, 태극삼, 곡삼, 산양삼, 장뇌삼, 산삼배양근, 새싹인삼 및 산삼으로 이루어진 군으로부터 선택되는 하나 이상의 인삼일 수 있으나, 이에 제한되지 않는다.In the method for producing a ginseng concentrate of the present invention, the ginseng may be one or more ginseng selected from the group consisting of fresh ginseng, red ginseng, dried ginseng, white ginseng, black ginseng, Taegeuk ginseng, gok ginseng, wild ginseng, Jangnoe ginseng, wild ginseng cultured root, sprout ginseng, and wild ginseng. , but not limited thereto.

또한, 본 발명의 인삼 농축액의 제조방법에서, 상기 (1)단계의 인삼 추출액은 바람직하게는 인삼을 분쇄한 인삼 분쇄물에 60~80%(v/v) 주정을 7~9배(v/w) 첨가하여 55~60℃에서 1~3시간 동안 추출한 후 여과하여 제조할 수 있으며, 더욱 바람직하게는 인삼을 분쇄한 인삼 분쇄물에 70%(v/v) 주정을 8배(v/w) 첨가하여 55~60℃에서 2시간 동안 추출한 후 여과하여, 인삼 내 유효성분을 보다 최대로 추출할 수 있었다.In addition, in the method for producing a ginseng concentrate of the present invention, the ginseng extract of step (1) is preferably 7 to 9 times (v/v) 60 to 80% (v/v) alcohol in the pulverized ginseng pulverized ginseng. w) It can be added and extracted at 55 to 60° C. for 1-3 hours and then filtered, and more preferably, 70% (v/v) alcohol is added to the ground ginseng pulverized ginseng 8 times (v/w). ) was added and extracted for 2 hours at 55 to 60° C., and then filtered to maximize the extraction of active ingredients in ginseng.

또한, 본 발명의 인삼 농축액의 제조방법에서, 상기 (2)단계의 농축은 바람직하게는 인삼 추출액을 판형증발농축기로 50~60℃에서 67~71 brix로 농축할 수 있으며, 더욱 바람직하게는 인삼 추출액을 판형증발농축기로 55℃에서 69~70 brix로 농축할 수 있다. 인삼 추출액을 상기와 같은 조건으로 농축하는 것이 특정 진세노사이드 함량을 높이면서 보다 효율적으로 농축할 수 있었다.In addition, in the method for producing the ginseng concentrate of the present invention, the concentration in step (2) may preferably concentrate the ginseng extract to 67 to 71 brix at 50 to 60 ° C with a plate-type evaporator, more preferably ginseng The extract can be concentrated to 69~70 brix at 55℃ with a plate-type evaporator. Concentrating the ginseng extract under the same conditions as above was able to more efficiently concentrate while increasing the specific ginsenoside content.

또한, 본 발명의 인삼 농축액의 제조방법에서, 상기 (2)단계의 농축한 농축액의 특정 진세노사이드 함량을 더 높이기 위해 열처리하는 단계를 추가로 포함할 수 있는데, 상기 열처리는 보다 구체적으로는 85~95℃에서 5~7시간 동안 열처리할 수 있으며, 더욱 구체적으로는 85~95℃에서 6시간 동안 열처리할 수 있다. In addition, in the method for producing a ginseng concentrate of the present invention, it may further include a step of heat-treating to further increase the specific ginsenoside content of the concentrated solution of step (2), wherein the heat treatment is more specifically 85 It can be heat-treated at ~95 ℃ for 5-7 hours, more specifically, it can be heat-treated at 85-95 ℃ for 6 hours.

본 발명의 인삼 농축액의 제조방법은, 보다 구체적으로는The manufacturing method of the ginseng concentrate of the present invention, more specifically,

(1) 인삼을 분쇄한 인삼 분쇄물에 60~80%(v/v) 주정을 7~9배(v/w) 첨가하여 55~60℃에서 1~3시간 동안 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및(1) 60~80% (v/v) alcohol is added 7~9 times (v/w) to the pulverized ginseng product, extracted at 55~60℃ for 1~3 hours, and filtered to obtain a ginseng extract manufacturing; and

(2) 상기 (1)단계의 제조한 인삼 추출액을 판형증발농축기로 50~60℃에서 67~71 brix로 농축하는 단계를 포함할 수 있으며,(2) may include the step of concentrating the ginseng extract prepared in step (1) to 67 to 71 brix at 50 to 60 ° C with a plate-type evaporator,

더욱 구체적으로는more specifically

(1) 인삼을 분쇄한 인삼 분쇄물에 70%(v/v) 주정을 8배(v/w) 첨가하여 55~60℃에서 2시간 동안 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및(1) adding 70% (v/v) alcohol to the pulverized ginseng product by 8 times (v/w), extracting it at 55 to 60° C. for 2 hours, and then filtering to prepare a ginseng extract; and

(2) 상기 (1)단계의 제조한 인삼 추출액을 판형증발농축기로 55℃에서 69~70 brix로 농축하는 단계를 포함할 수 있다.(2) It may include the step of concentrating the ginseng extract prepared in step (1) at 55 ° C. to 69 to 70 brix with a plate-type evaporator.

본 발명의 인삼 농축액의 제조방법에서, 상기 (1)단계의 인삼은 흑삼, 마타리 뿌리, 여뀌잎 및 고마리잎을 혼합한 흑삼 혼합물에 물을 첨가하여 추출한 후 여과한 식물 혼합 추출물에 침지한 후 꺼낸 인삼일 수 있는데,In the method for producing a ginseng concentrate of the present invention, the ginseng of step (1) is extracted by adding water to a black ginseng mixture mixed with black ginseng, matari root, marigold leaf, and fern leaf, and then immersed in the filtered plant mixed extract and taken out It may be ginseng,

상기 인삼을 사용할 경우, 인삼 농축액의 제조방법은, 보다 구체적으로는When using the ginseng, the method for preparing the ginseng concentrate is more specifically

(1) 흑삼 혼합물 총 중량 기준으로, 흑삼 48~52 중량%, 마타리 뿌리 18~22 중량%, 여뀌잎 18~22 중량% 및 고마리잎 8~12 중량%를 혼합한 흑삼 혼합물에 물을 7~9배(v/w) 첨가하여 80~90℃에서 1~3시간 동안 추출한 후 여과한 식물 혼합 추출물에 인삼을 45~55℃에서 2~4시간 동안 침지한 후 꺼내고 분쇄한 인삼 분쇄물에 60~80%(v/v) 주정을 7~9배(v/w) 첨가하여 55~60℃에서 1~3시간 동안 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및(1) Based on the total weight of the black ginseng mixture, water 7~ Add 9 times (v/w), extract at 80~90℃ for 1~3 hours, and then soak ginseng in the filtered plant mixed extract for 2~4 hours at 45~55℃, take it out and add 60 to the pulverized ginseng powder Preparing a ginseng extract by adding ~80% (v/v) alcohol 7 to 9 times (v/w), extracting it at 55 to 60° C. for 1 to 3 hours, and then filtering; and

(2) 상기 (1)단계의 제조한 인삼 추출액을 판형증발농축기로 50~60℃에서 67~71 brix로 농축하는 단계를 포함할 수 있으며,(2) may include the step of concentrating the ginseng extract prepared in step (1) to 67 to 71 brix at 50 to 60 ° C with a plate-type evaporator,

더욱 구체적으로는more specifically

(1) 흑삼 혼합물 총 중량 기준으로, 흑삼 50 중량%, 마타리 뿌리 20 중량%, 여뀌잎 20 중량% 및 고마리잎 10 중량%를 혼합한 흑삼 혼합물에 물을 8배(v/w) 첨가하여 85℃에서 2시간 동안 추출한 후 여과한 식물 혼합 추출물에 인삼을 50℃에서 3시간 동안 침지한 후 꺼내고 분쇄한 인삼 분쇄물에 70%(v/v) 주정을 8배(v/w) 첨가하여 55~60℃에서 2시간 동안 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및(1) Based on the total weight of the black ginseng mixture, water was added 8 times (v/w) to the black ginseng mixture mixed with 50% by weight of black ginseng, 20% by weight of matari root, 20% by weight of ginseng leaves, and 10% by weight of fern leaves to 85 After extraction at ℃ for 2 hours, ginseng was immersed in the filtered plant mixed extract at 50℃ for 3 hours, taken out, and 70% (v/v) alcohol was added 8 times (v/w) to the pulverized ginseng pulverized product, 55 Preparing a ginseng extract by filtration after extraction at ~60 ℃ for 2 hours; and

(2) 상기 (1)단계의 제조한 인삼 추출액을 판형증발농축기로 55℃에서 69~70 brix로 농축하는 단계를 포함할 수 있다.(2) It may include the step of concentrating the ginseng extract prepared in step (1) at 55 ° C. to 69 to 70 brix with a plate-type evaporator.

본 발명의 상기와 같이 제조된 식물 혼합 추출물을 이용하여 인삼을 침지하는 것이 인삼의 유효성분의 용출을 최소화하면서 인삼 내 진세노사이드 함량을 더욱 증진시키고, 부드럽고 기호도가 우수한 인삼으로 전처리할 수 있었다.Immersion of ginseng using the plant mixed extract prepared as described above of the present invention minimizes the elution of the active ingredient of ginseng, while further enhancing the ginsenoside content in ginseng, and it was possible to pre-treat with ginseng having a soft and excellent taste.

본 발명은 또한, 상기 방법으로 제조된 인삼 농축액을 제공한다.The present invention also provides a ginseng concentrate prepared by the above method.

본 발명의 인삼 농축액은 프로사포게닌 진세노사이드 함량이 증진되는 것을 특징으로 하며, 상기 프로사포게닌 진세노사이드는 Rk1, Rg5 및 Rg3로 이루어진 군으로부터 선택되는 하나 이상의 진세노사이드일 수 있으나, 이에 제한되지 않는다.The ginseng concentrate of the present invention is characterized in that the content of prosapogenin ginsenoside is increased, and the prosapogenin ginsenoside may be one or more ginsenosides selected from the group consisting of Rk1, Rg5 and Rg3, not limited

이하, 본 발명의 제조예 및 실시예를 들어 상세히 설명한다. 단, 하기 제조예 및 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 제조예 및 실시예에 한정되는 것은 아니다.Hereinafter, production examples and examples of the present invention will be described in detail. However, the following Preparation Examples and Examples only illustrate the present invention, the content of the present invention is not limited to the following Preparation Examples and Examples.

제조예 1. 홍삼 농축액(판형증발농축)Preparation Example 1. Red ginseng concentrate (plate-type evaporation concentration)

(1) 인삼을 100℃에서 2시간 동안 1회 증자한 후 50℃에서 수분 함량이 15% 이내가 되도록 건조하여 홍삼을 제조하였다.(1) Red ginseng was prepared by steaming ginseng once at 100° C. for 2 hours and then drying it at 50° C. so that the moisture content was within 15%.

(2) 상기 (1)단계의 제조한 홍삼을 분쇄한 홍삼 분쇄물에 70%(v/v) 주정을 8배(v/w) 첨가하여 55~60℃에서 2시간 동안 추출한 후 여과하여 홍삼 추출액을 제조하였다.(2) 70% (v/v) alcohol was added 8 times (v/w) to the pulverized red ginseng obtained by pulverizing the red ginseng prepared in step (1), extracted at 55 to 60° C. for 2 hours, filtered and red ginseng An extract was prepared.

(3) 상기 (2)단계의 제조한 홍삼 추출액을 판형증발농축기로 55℃에서 70 brix로 농축하였다.(3) The red ginseng extract prepared in step (2) was concentrated to 70 brix at 55°C using a plate-type evaporator.

제조예 2. 홍삼 농축액(추출물 침지-판형증발농축-열처리)Preparation Example 2. Red ginseng concentrate (extract immersion-plate-type evaporation concentration-heat treatment)

(1) 인삼을 100℃에서 2시간 동안 1회 증자한 후 50℃에서 수분 함량이 15% 이내가 되도록 건조하여 홍삼을 제조하였다.(1) Red ginseng was prepared by steaming ginseng once at 100° C. for 2 hours and then drying it at 50° C. so that the moisture content was within 15%.

(2) 흑삼 혼합물 총 중량 기준으로, 흑삼 50 중량%, 마타리 뿌리(가얌취) 20 중량%, 여뀌잎 20 중량% 및 고마리잎 10 중량%를 혼합한 흑삼 혼합물에 정제수를 8배(v/w) 첨가하여 85℃에서 2시간 동안 추출한 후 여과하여 식물 혼합 추출물을 제조하였다.(2) Based on the total weight of the black ginseng mixture, purified water was added 8 times (v/w ) was added and extracted at 85° C. for 2 hours, followed by filtration to prepare a mixed plant extract.

(3) 상기 (1)단계의 제조한 홍삼을 상기 (2)단계의 제조한 식물 혼합 추출물에 50℃에서 3시간 동안 침지한 후 꺼내었다.(3) The red ginseng prepared in step (1) was immersed in the plant mixed extract prepared in step (2) at 50° C. for 3 hours and then taken out.

(4) 상기 (3)단계의 침지한 후 꺼낸 홍삼을 분쇄한 홍삼 분쇄물에 70%(v/v) 주정을 8배(v/w) 첨가하여 55~60℃에서 2시간 동안 추출한 후 여과하여 홍삼 추출액을 제조하였다.(4) 70% (v/v) alcohol was added 8 times (v/w) to the pulverized red ginseng ground red ginseng taken out after immersion in step (3), extracted at 55 to 60° C. for 2 hours, and filtered Thus, a red ginseng extract was prepared.

(5) 상기 (24)단계의 제조한 홍삼 추출액을 판형증발농축기로 55℃에서 70 brix로 농축하고 85~95℃에서 6시간 동안 열처리하였다.(5) The red ginseng extract prepared in step (24) was concentrated to 70 brix at 55°C using a plate-type evaporator, and heat-treated at 85-95°C for 6 hours.

비교예 1. 홍삼 농축액(감압농축)Comparative Example 1. Red Ginseng Concentrate (Pressure Concentration)

(1) 인삼을 100℃에서 2시간 동안 1회 증자한 후 50℃에서 수분 함량이 15% 이내가 되도록 건조하여 홍삼을 제조하였다.(1) Red ginseng was prepared by steaming ginseng once at 100° C. for 2 hours and then drying it at 50° C. so that the moisture content was within 15%.

(2) 상기 (1)단계의 제조한 홍삼을 분쇄한 홍삼 분쇄물에 70%(v/v) 주정을 8배(v/w) 첨가하여 55~60℃에서 2시간 동안 추출한 후 여과하여 홍삼 추출액을 제조하였다.(2) 70% (v/v) alcohol was added 8 times (v/w) to the pulverized red ginseng obtained by pulverizing the red ginseng prepared in step (1), extracted at 55 to 60° C. for 2 hours, filtered and red ginseng An extract was prepared.

(3) 상기 (2)단계의 제조한 홍삼 추출액을 감압농축기로 65~70℃에서 70 brix로 농축하였다.(3) The red ginseng extract prepared in step (2) was concentrated to 70 brix at 65-70° C. with a reduced pressure concentrator.

제조예 3. 흑삼 농축액Preparation Example 3. Black Ginseng Concentrate

(1) 흑삼을 분쇄한 흑삼 분쇄물에 70%(v/v) 주정을 8배(v/w) 첨가하여 55~60℃에서 2시간 동안 추출한 후 여과하여 흑삼 추출액을 제조하였다.(1) 70% (v/v) alcohol was added 8 times (v/w) to the ground black ginseng ground black ginseng, extracted at 55 to 60° C. for 2 hours, and then filtered to prepare a black ginseng extract.

(2) 상기 (1)단계의 제조한 흑삼 추출액을 판형증발농축기로 55℃에서 69 brix로 농축하였다.(2) The black ginseng extract prepared in step (1) was concentrated to 69 brix at 55° C. using a plate-type evaporator.

1. 홍삼 농축액 제조1. Preparation of red ginseng concentrate

(1) 홍삼 준비(1) Preparation of red ginseng

인삼을 100℃에서 2시간 동안 1회 증자한 후 50℃에서 수분 함량이 15% 이내가 되도록 건조하여 홍삼을 제조하였다.Red ginseng was prepared by steaming ginseng once at 100° C. for 2 hours, and then drying it at 50° C. so that the moisture content was within 15%.

(2) 침지(2) immersion

상기 제조한 홍삼을 식물 혼합 추출물(제조예 2의 (2)단계)에 50℃에서 3시간 동안 침지한 후 꺼내었다.The prepared red ginseng was immersed in the plant mixed extract (step (2) of Preparation Example 2) at 50° C. for 3 hours and then taken out.

(3) 주정 추출(3) alcohol extraction

상기 침지한 후 꺼낸 홍삼을 분쇄한 홍삼 분쇄물에 70%(v/v) 주정을 8배(v/w) 첨가하여 55~60℃에서 2시간 동안 추출한 후 여과하여 홍삼 추출액을 제조하였다.Red ginseng extracted after immersion was added to the pulverized red ginseng powder by adding 70% (v/v) alcohol 8 times (v/w), followed by extraction at 55 to 60° C. for 2 hours, followed by filtration to prepare a red ginseng extract.

(4) 복합농축기술(4) Complex Concentration Technology

통상적으로 사용하는 감압농축기를 사용한 감압농축방식은 농축 과정에서 탱크의 이중 자켓 내부에 스팀이 공급되고, 이때 추출액의 증발과정에서 자켓의 접촉면에서 순간적인 열충격으로 지속적인 성분 파괴의 가능성이 있다(도 1).In the vacuum concentration method using a conventionally used vacuum concentrator, steam is supplied to the inside of the double jacket of the tank during the concentration process, and there is a possibility of continuous component destruction due to instantaneous thermal shock on the contact surface of the jacket during the evaporation of the extract (Fig. 1). ).

본 발명에서 사용한 판형증발농축기(plate evaporator)는 평판식가열농축기, 플레이트농축기 또는 판형증발농축시스템이라고 불리며, 상기와 같은 감압농축의 단점을 보완하여 추출액이 열에 의해 손상을 받는 것을 최소화한다. 또한, 다수의 플레이트 사이에 형성된 공간으로부터 가열, 냉각, 증발, 응축이 이루어지는 방식으로 감압농축방식에 비해 효율성이 좋은 잇점이 있다.The plate evaporator used in the present invention is called a plate type heating concentrator, a plate concentrator or a plate type evaporator, and it compensates for the disadvantages of the reduced pressure concentration as described above to minimize damage to the extract by heat. In addition, in a manner in which heating, cooling, evaporation, and condensation are performed from the space formed between a plurality of plates, there is an advantage of better efficiency compared to the reduced pressure concentration method.

상기 제조한 홍삼 추출액을 다수의 플레이트가 적층되어 있는 판형증발농축기의 하부에 추출액을 공급하면, 상부에서 공급되는 스팀으로 인해 추출액으로부터 수증기가 발생하여 증기로 배출되고, 스팀은 냉각수의 공급으로 응축되어 응축수 형태로 배출되고, 증기로 배출되고 남은 추출액은 농축된 형태로 얻어지게 되어, 최종 70 brix의 홍삼 농축액을 회수하였다(도 1 및 표 1). When the red ginseng extract prepared above is supplied to the lower part of the plate-type evaporator on which a plurality of plates are stacked, water vapor is generated from the extract due to the steam supplied from the upper portion and discharged as steam, and the steam is condensed by supply of cooling water. It was discharged in the form of condensed water, and the extract remaining after being discharged as steam was obtained in a concentrated form, and a final 70 brix red ginseng concentrate was recovered (FIG. 1 and Table 1).

판형증발농축기 가동조건Operation conditions of plate evaporator 구분division 유속flow rate 농축(brix)concentrate (brix) 온도(℃)Temperature (℃) 압력(bar)pressure (bar) 스팀 공급steam supply 1,300~1,400 kg/h1,300 to 1,400 kg/h -- -- 2.5~3.52.5~3.5 추출액 공급supply of extract 2,900~3,100 kg/h2,900~3,100 kg/h 12~1612-16 8~128-12 1.2~1.81.2~1.8 냉각수 공급coolant supply 3~5 m3/h3-5 m 3 /h -- 8~128-12 1.8~2.21.8~2.2 응축수 회수Condensate recovery 3~5 m3/h3-5 m 3 /h 15~1815-18 15~1815-18 1.3~1.71.3~1.7 농축액 회수Concentrate recovery 550~570 kg/h550-570 kg/h 65~7065-70 52~5752-57 1.3~1.71.3~1.7

(5) 열처리(5) heat treatment

상기 회수한 홍삼 농축액의 진세노사이드 함량을 더 높이기 위해, 열처리 시스템으로 옮겨 85~95℃에서 6시간 동안 열처리하였다(도 2).In order to further increase the ginsenoside content of the recovered red ginseng concentrate, it was transferred to a heat treatment system and heat-treated at 85-95° C. for 6 hours ( FIG. 2 ).

2. 진세노사이드 함량 측정 방법2. Method for measuring ginsenoside content

진세노사이드의 함량 분석은 UVD(Ultra Visible Detector)가 장착된 HPLC(High Performance Liquid Chromatography)로 분석하였고, 분석조건은 하기 표 2와 같고, 함량 계산식은 하기 식과 같다.The content analysis of ginsenosides was analyzed by High Performance Liquid Chromatography (HPLC) equipped with UVD (Ultra Visible Detector), the analysis conditions are as shown in Table 2 below, and the content calculation formula is as follows.

진세노사이드 함량(㎎/g) = S ××시료채취량(g)Ginsenoside content (mg/g) = S × × Sample amount (g)

S: 시험용액 중 개별 진세노사이드 농도(㎎/㎖)S: Individual ginsenoside concentration in test solution (mg/ml)

a: 시험용액의 전량(㎖)a: Total amount of test solution (ml)

b: 희석배수b: dilution factor

진세노사이드 분석조건Ginsenoside analysis conditions 장비equipment HPLC Agilent 1260 seriesHPLC Agilent 1260 series 검출기detector DAD detector(203 nm)DAD detector (203 nm) 컬럼column Prontosil 120-5-C18-ace-EPS
(4.6 mm×250 mm, 5.0 ㎛)
Prontosil 120-5-C18-ace-EPS
(4.6 mm×250 mm, 5.0 μm)
컬럼온도column temperature 40℃40℃ 이동상mobile phase Acetonitrile:D.W. (gradient)Acetonitrile: D.W. (gradient) 유속flow rate 1.0 ㎖/min1.0 ml/min 주입 용량infusion dose 10 ㎕10 μl

실시예 1. 식물 혼합 추출물에 침지 조건 확립Example 1. Establishment of immersion conditions in plant mixed extract

홍삼을 식물 혼합 추출물에 침지 온도 및 시간별 수분 함량과 홍삼성분 유출 정도를 비교하였다.The water content and the degree of red ginseng component leakage were compared by immersion temperature and time in the plant-mixed extract of red ginseng.

수분 함량의 결과, 처리온도가 10℃ 및 30℃일 경우, 6시간 동안 침지하여도 홍삼 내 수분 함량이 50% 이하였으며, 50℃ 이상으로 침지하여야 침지 효율이 좋은 것으로 나타났다(표 3).As a result of the moisture content, when the treatment temperature was 10°C and 30°C, the moisture content in red ginseng was less than 50% even after immersion for 6 hours, and immersion at 50°C or higher showed good immersion efficiency (Table 3).

홍삼의 수분 함량(%) 변화Change in water content (%) of red ginseng 처리온도processing temperature 처리시간processing time 처리 후 홍삼의 수분 함량(%)Water content of red ginseng after treatment (%) 10℃10℃ 1시간1 hours 19.0419.04 2시간2 hours 23.8323.83 3시간3 hours 25.4725.47 6시간6 hours 29.6929.69 30℃30℃ 1시간1 hours 16.4616.46 2시간2 hours 26.0426.04 3시간3 hours 30.8330.83 6시간6 hours 42.2842.28 50℃50℃ 1시간1 hours 39.6339.63 2시간2 hours 67.8567.85 3시간3 hours 74.8074.80 6시간6 hours 85.2385.23 70℃70℃ 1시간1 hours 16.5716.57 2시간2 hours 61.6061.60 3시간3 hours 80.4480.44 6시간6 hours >90>90

침지 조건별 침지액과 침지 후 원료 홍삼의 Brix와 3종(Rb1, Rg1, Rg3의 합) 및 흑삼 3종(Rk1, Rg5, Rg3 합)의 함량을 비교한 결과는 하기 표 4와 같다.The results of comparing the contents of the immersion liquid for each immersion condition and the Brix of raw red ginseng after immersion, 3 types (sum of Rb1, Rg1, Rg3) and 3 types of black ginseng (sum of Rk1, Rg5, Rg3) are shown in Table 4 below.

그 결과, 침지 온도 및 시간이 길어질수록 침지액 내 진세노사이드의 유출 정도가 높아짐을 확인하였다. 또한, 침지 후 원료 홍삼의 흑삼 3종 진세노사이드 함량은 50℃에서 3시간 침지하는 것이 가장 높은 것으로 나타나, 상기 조건으로 침지하는 것으로 최종 결정하였다.As a result, it was confirmed that as the immersion temperature and time increased, the degree of outflow of ginsenosides in the immersion solution increased. In addition, the three types of black ginseng ginsenoside content of raw material red ginseng after immersion was found to be the highest when immersed at 50° C. for 3 hours, and immersion under the above conditions was finally determined.

홍삼의 성분 유출 정도 확인Check the level of leakage of red ginseng ingredients 검체sample 침지액immersion liquid 침지 후 원료 홍삼Raw red ginseng after immersion 온도temperature 시간hour Brix 변화Brix change 3종(㎎/g)3 types (mg/g) 흑삼3종
(㎎/g)
3 types of black ginseng
(mg/g)
3종(㎎/g)3 types (mg/g) 흑삼3종
(㎎/g)
3 types of black ginseng
(mg/g)
대조군control 10.2610.26 1.2031.203 1.5131.513 4.524.52 0.190.19 10℃10℃ 1시간1 hours 10.3110.31 1.231.23 1.551.55 4.514.51 0.170.17 2시간2 hours 10.3410.34 1.231.23 1.551.55 4.494.49 0.160.16 3시간3 hours 10.4310.43 1.221.22 1.571.57 4.504.50 0.150.15 6시간6 hours 10.5010.50 1.251.25 1.501.50 4.484.48 0.200.20 30℃30℃ 1시간1 hours 10.3310.33 1.241.24 1.561.56 4.534.53 0.220.22 2시간2 hours 10.6210.62 1.251.25 1.571.57 4.544.54 0.240.24 3시간3 hours 10.8710.87 1.271.27 1.591.59 4.504.50 0.230.23 6시간6 hours 10.9010.90 1.301.30 1.571.57 4.554.55 0.210.21 50℃50℃ 1시간1 hours 10.5610.56 1.231.23 1.561.56 4.494.49 0.250.25 2시간2 hours 11.2811.28 1.301.30 1.651.65 4.594.59 0.330.33 3시간3 hours 11.6011.60 1.341.34 1.691.69 4.624.62 0.480.48 6시간6 hours 11.8911.89 1.371.37 1.651.65 4.894.89 0.370.37 70℃70℃ 1시간1 hours 10.4410.44 1.391.39 1.751.75 4.574.57 0.270.27 2시간2 hours 11.8411.84 1.361.36 1.741.74 4.684.68 0.370.37 3시간3 hours 13.1913.19 1.431.43 1.81.8 4.594.59 0.390.39 6시간6 hours 13.8313.83 1.471.47 2.032.03 4.514.51 0.250.25

실시예 2. 농축방법에 따른 홍삼 농축액의 pH 및 갈색도Example 2. pH and brownness of red ginseng concentrate according to the concentration method

감압농축과 판형증발농축 방식으로 농축한 홍삼 농축액(70 brix)의 pH와 갈색도(탁도)를 비교한 결과는 하기 표 5와 같다. 그 결과, pH는 판형증발농축 홍삼 농축액이 더 높게 나타났고, 갈색도는 더 낮게 나타났다. 갈색도 측정은 농축액을 증류수로 100배 희석 후 2시간 동안 상온에서 정치하고 여과한 후 420 nm에서 흡광도를 분광광도계를 이용하여 측정하였다. 또한, 농축액을 100배 희석한 희석액을 비교하였을 때, 감압농축한 농축액보다 판형증발농축한 농축액이 더 맑은 것으로 나타났다(도 3).The results of comparing the pH and brownness (turbidity) of the red ginseng concentrate (70 brix) concentrated by vacuum concentration and plate-type evaporation are shown in Table 5 below. As a result, the pH was higher in the plate-type evaporation-concentrated red ginseng concentrate, and the brownness was lower. For brownness measurement, the concentrate was diluted 100 times with distilled water, left at room temperature for 2 hours, filtered, and then absorbance at 420 nm was measured using a spectrophotometer. In addition, when the 100-fold dilution of the concentrated solution was compared, the plate-type evaporation concentrated solution was clearer than the reduced pressure concentrated solution (FIG. 3).

농축방법에 따른 홍삼 농축액의 pH 및 갈색도pH and brownness of red ginseng concentrate according to concentration method 구분division 감압증발농축reduced pressure evaporation 판형증발농축plate-type evaporation pHpH 4.74.7 4.94.9 갈색도brown degree 0.50.5 0.30.3 비고note 약간 혼탁slightly cloudy 감압증발보다 맑음Clearer than vacuum evaporation

실시예 3. 농축방법에 따른 홍삼 및 흑삼 농축액의 수율 및 품질 특성Example 3. Yield and quality characteristics of red ginseng and black ginseng concentrate according to the concentration method

감압농축과 판형증발농축 방식으로 농축한 홍삼 및 흑삼 농축액의 생산 효율과 진세노사이드 함량을 비교한 결과는 하기 표 6과 같다.The results of comparing the production efficiency and ginsenoside content of red ginseng and black ginseng concentrates concentrated by vacuum concentration and plate-type evaporation concentration are shown in Table 6 below.

통상적인 감압농축 시 스팀온도가 갑자기 높아져, 농축액의 품질에 영향을 주는 반면, 본 발명에서 사용한 판형증발농축방식은 비교적 낮은 온도에서 농축이 가능하고 스팀온도에 영향을 받지 않는다. 또한, 생산효율도 약 8배 높고, 추출액을 농축액으로 제조 시 진세노사이드 손실없이 농축시킬 수 있음을 확인하였다(표 6).During conventional vacuum concentration, the steam temperature suddenly increases, which affects the quality of the concentrate, whereas the plate-type evaporation method used in the present invention allows concentration at a relatively low temperature and is not affected by the steam temperature. In addition, it was confirmed that the production efficiency is also about 8 times higher, and can be concentrated without loss of ginsenosides when the extract is prepared as a concentrate (Table 6).

홍삼 및 흑삼 농축액의 농축 수율 및 진세노사이드 함량 비교Comparison of concentrated yield and ginsenoside content of red ginseng and black ginseng concentrate 구분division 추출액
(20 brix)
extract
(20 brix)
농축액(69 brix)Concentrate (69 brix) 추출액 농축시 진세노사이드 이론 함량Theoretical content of ginsenosides in extract concentration
감압증발농축reduced pressure evaporation 판형증발농축plate-type evaporation 농축온도(℃)Concentration temperature (℃) -- 65~70℃65~70℃ 55~60℃55~60℃ -- 스팀온도 영향을 받는 노출시간Exposure time affected by steam temperature -- 1~4시간1-4 hours 없음doesn't exist -- 생산효율
(농축액량/시간 당)
production efficiency
(per concentrate volume/hour)
-- 300 kg/hr300 kg/hr 2,500 kg/hr2,500 kg/hr --
진세노사이드 함량(mg/g)Ginsenoside content (mg/g) 홍삼 농축액*Red Ginseng Concentrate* 3종합3 total 4.444.44 13.9213.92 15.0915.09 15.3315.33 Rg1Rg1 1.041.04 3.143.14 3.543.54 3.573.57 Rb1Rb1 3.273.27 10.4510.45 11.0611.06 11.2711.27 Rg3Rg3 0.150.15 0.330.33 0.490.49 0.500.50 흑삼 농축액*Black Ginseng Concentrate* 3종합3 total 3.713.71 12.1412.14 12.4912.49 12.4912.49 Rg3Rg3 1.291.29 4.344.34 4.454.45 4.454.45 Rg5Rg5 1.091.09 3.613.61 3.803.80 3.763.76 Rk1Rk1 1.241.24 4.094.09 4.244.24 4.284.28

* 홍삼 농축액은 기본 3종(Rg1, Rb1, Rg3)의 함량 비교, 흑삼 농축액은 프로사포게닌 진세노사이드 중 3종(Rg3, Rk1, Rg5)으로 진세노사이드 함량 비교* Comparison of ginsenoside contents with 3 types of prosapogenin ginsenosides (Rg3, Rk1, Rg5) in black ginseng concentrate

실시예 4. 농축방식에 따른 홍삼 농축액의 비사포닌 성분 비교Example 4. Comparison of non-saponin components of red ginseng concentrate according to concentration method

농축방식에 따른 홍삼 농축액의 비사포닌 성분을 비교한 결과는 하기 표 7과 같다. 그 결과, 감압증발 농축방식에 비해 판형증발농축기로 농축한 농축액이 폴리페놀 및 플라보노이드 함량과 총당, 산성다당체 및 조사포닌 함량이 더 높게 나타났다.The results of comparing the non-saponin components of the red ginseng concentrate according to the concentration method are shown in Table 7 below. As a result, the contents of polyphenols and flavonoids, total sugars, acidic polysaccharides, and saponin were higher in the concentrate concentrated by the plate type evaporator compared to the vacuum evaporation method.

농축 방식에 따른 비사포닌 성분 비교Comparison of non-saponin components according to the concentration method 구분division 감압증발 농축액vacuum evaporation concentrate 판형증발 농축액Plate Evaporation Concentrate 총 폴리페놀(㎎/g)Total polyphenols (mg/g) 8.458.45 9.199.19 총 플라보노이드(㎎/g)Total flavonoids (mg/g) 3.013.01 3.473.47 총당(g/100 g)Total sugar (g/100 g) 33.4533.45 35.6735.67 산성다당체(g/100 g)Acidic polysaccharide (g/100 g) 9.899.89 10.1110.11 조사포닌(g/100 g)saponin (g/100 g) 9.659.65 10.3510.35 pHpH 4.7~4.94.7~4.9 4.8~5.04.8~5.0 갈색도brown degree 0.50.5 0.30.3

실시예 5. 홍삼 농축액의 관능검사Example 5. Sensory test of red ginseng concentrate

관능검사는 성인 40명을 대상으로 각각의 제조방법을 달리한 제조예 1 및 2와 비교예 1의 홍삼 농축액을 기호에 맞게 희석하여 섭취하게 한 후, 이취, 쓴맛, 전체적인 선호도를 평가하게 하였다. 이취 및 쓴맛은 그 정도가 낮을수록 높은 점수를 주게 하였고, 전체적인 선호도는 더 선호하는 농축액을 더 높은 점수를 주게 하여, 5점 척도법으로 평가하였다.For the sensory test, 40 adults were diluted with the red ginseng concentrates of Preparation Examples 1 and 2 and Comparative Example 1 with different manufacturing methods according to their preference to ingest, and then, off-flavor, bitter taste, and overall preference were evaluated. Off-flavor and bitterness were given a higher score as the degree was lower, and overall preference gave a higher score to the more preferred concentrate, and was evaluated on a 5-point scale.

제조방법에 따른 홍삼 농축액의 관능검사Sensory test of red ginseng concentrate according to manufacturing method 구분division 이취off-flavor 쓴맛bitter 전체적인 기호도overall sign 제조예 1Preparation Example 1 3.93.9 4.14.1 4.04.0 제조예 2Preparation 2 4.14.1 4.24.2 4.34.3 비교예 1Comparative Example 1 3.53.5 3.53.5 3.63.6

그 결과, 제조예 2의 추출물 침지, 판형증발농축 및 열처리 과정을 거쳐 제조한 홍삼 농축액이 가장 높은 선호도를 나타내었고, 비교예 1이 가장 낮은 선호도를 나타내었다.As a result, the red ginseng concentrate prepared through the extract immersion, plate-type evaporation, and heat treatment processes of Preparation Example 2 showed the highest preference, and Comparative Example 1 showed the lowest preference.

실시예 6. 연속식 열처리 공정에 따른 농축액의 진세노사이드 변화Example 6. Changes in ginsenosides in the concentrate according to the continuous heat treatment process

판형증발농축한 홍삼 및 흑삼 농축액의 연속식 열처리 시 열처리 시간에 따른 프로사포게닌 진세노사이드 함량을 비교한 결과는 하기 표 9 및 10과 같다.Tables 9 and 10 show the results of comparison of prosapogenin ginsenoside content according to heat treatment time during continuous heat treatment of plate-type evaporation-concentrated red ginseng and black ginseng concentrates.

홍삼 농축액의 열처리 시간에 따른 진세노사이드 함량(㎎/g)Ginsenoside content (mg/g) according to heat treatment time of red ginseng concentrate 열처리 시간heat treatment time Rg3Rg3 Rk1Rk1 Rg5Rg5 3종 합3 kinds of sum 함량content 증가율(%)Increase (%) 함량content 증가율(%)Increase (%) 함량content 증가율(%)Increase (%) 함량content 증가율(%)Increase (%) 대조구control 1.191.19 -- 1.041.04 -- 1.041.04 -- 3.273.27 -- 30분30 minutes 1.331.33 12.012.0 1.211.21 15.915.9 1.141.14 10.110.1 3.683.68 12.712.7 1시간1 hours 1.381.38 16.316.3 1.291.29 24.024.0 1.281.28 24.024.0 3.963.96 21.221.2 2시간2 hours 1.491.49 25.525.5 1.411.41 35.035.0 1.441.44 38.638.6 4.344.34 32.732.7 3시간3 hours 1.751.75 47.347.3 1.761.76 69.069.0 1.731.73 67.367.3 5.255.25 60.560.5 4시간4 hours 1.871.87 56.956.9 1.911.91 82.682.6 1.911.91 84.584.5 5.685.68 73.973.9 6시간6 hours 2.132.13 79.179.1 2.222.22 112.4112.4 2.252.25 117.0117.0 6.596.59 101.7101.7

흑삼 농축액의 열처리 시간에 따른 진세노사이드 함량(㎎/g)Ginsenoside content (mg/g) according to heat treatment time of black ginseng concentrate 열처리 시간heat treatment time Rg3Rg3 Rk1Rk1 Rg5Rg5 3종 합3 kinds of sum 함량content 증가율(%)Increase (%) 함량content 증가율(%)Increase (%) 함량content 증가율(%)Increase (%) 함량content 증가율(%)Increase (%) 대조구control 4.454.45 -- 3.803.80 -- 4.244.24 -- 12.4912.49 -- 30분30 minutes 4.544.54 2.002.00 3.943.94 3.663.66 4.324.32 1.831.83 12.7912.79 2.452.45 1시간1 hours 4.544.54 2.132.13 3.953.95 3.903.90 4.414.41 4.114.11 12.9012.90 3.343.34 2시간2 hours 4.654.65 4.584.58 4.104.10 7.857.85 4.584.58 8.078.07 13.3313.33 6.766.76 3시간3 hours 4.844.84 8.808.80 4.324.32 13.7913.79 4.814.81 13.4313.43 13.9713.97 11.8911.89 4시간4 hours 5.145.14 15.5715.57 4.624.62 21.6021.60 5.205.20 22.6122.61 14.9614.96 19.8019.80 6시간6 hours 5.755.75 29.2529.25 5.345.34 40.5940.59 5.945.94 40.1240.12 17.0317.03 36.3936.39

그 결과, 홍삼 및 흑삼 농축액에서 Rg3, Rk1 및 Rg5 함량 모두 6시간 열처리 시 가장 높은 함량을 나타내었다(도 4 및 5).As a result, all of the Rg3, Rk1 and Rg5 contents in the red ginseng and black ginseng concentrates showed the highest content during heat treatment for 6 hours ( FIGS. 4 and 5 ).

Claims (8)

(1) 흑삼, 마타리 뿌리, 여뀌잎 및 고마리잎을 혼합한 흑삼 혼합물에 물을 첨가하여 추출한 후 여과한 식물 혼합 추출물에 인삼을 침지한 후 꺼내고 분쇄한 인삼 분쇄물에 주정을 첨가하여 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및
(2) 다수의 플레이트가 적층되어 있는 판형증발농축기의 하부에 상기 (1)단계의 제조한 인삼 추출액을 공급하면, 상부에서 공급되는 스팀으로 인해 추출액으로부터 수증기가 발생하여 증기로 배출되고, 스팀은 냉각수의 공급으로 응축되어 응축수 형태로 배출되고, 증기로 배출되고 남은 추출액은 농축하는 단계를 포함하여 제조하는 것을 특징으로 하는 인삼 농축액의 제조방법.
(1) Water is added to a mixture of black ginseng mixed with black ginseng, matari root, fern leaf, and horseradish leaves for extraction, and ginseng is immersed in the filtered plant mixed extract and taken out. to prepare a ginseng extract; and
(2) When the ginseng extract prepared in step (1) is supplied to the lower part of the plate-type evaporator in which a plurality of plates are stacked, water vapor is generated from the extract due to the steam supplied from the upper part and discharged as steam, and the steam is A method for producing a ginseng concentrate, characterized in that it is condensed by supply of cooling water and discharged in the form of condensed water, and the extract remaining after being discharged as steam is prepared including the step of concentrating.
제1항에 있어서, 상기 인삼은 수삼, 홍삼, 건삼, 백삼, 흑삼, 태극삼, 곡삼, 산양삼, 장뇌삼, 산삼배양근, 새싹인삼 및 산삼으로 이루어진 군으로부터 선택되는 하나 이상의 인삼인 것을 특징으로 하는 인삼 농축액의 제조방법.According to claim 1, wherein the ginseng is fresh ginseng, red ginseng, dried ginseng, white ginseng, black ginseng, Taegeuk ginseng, gok ginseng, wild ginseng, camphor ginseng, wild ginseng cultured root, sprout ginseng, and ginseng concentrate, characterized in that at least one ginseng selected from the group consisting of wild ginseng. manufacturing method. 삭제delete 제1항에 있어서,
(1) 흑삼, 마타리 뿌리, 여뀌잎 및 고마리잎을 혼합한 흑삼 혼합물에 물을 첨가하여 추출한 후 여과한 식물 혼합 추출물에 인삼을 침지한 후 꺼내고 분쇄한 인삼 분쇄물에 60~80%(v/v) 주정을 7~9배(v/w) 첨가하여 55~60℃에서 1~3시간 동안 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및
(2) 다수의 플레이트가 적층되어 있는 50~60℃의 판형증발농축기의 하부에 상기 (1)단계의 제조한 인삼 추출액을 공급하면, 상부에서 공급되는 스팀으로 인해 추출액으로부터 수증기가 발생하여 증기로 배출되고, 스팀은 냉각수의 공급으로 응축되어 응축수 형태로 배출되고, 증기로 배출되고 남은 추출액은 67~71 brix로 농축하는 단계를 포함하여 제조하는 것을 특징으로 하는 인삼 농축액의 제조방법.
According to claim 1,
(1) Water is added to the black ginseng mixture mixed with black ginseng, matari root, ginseng leaf, and perilla leaf, followed by immersing ginseng in the filtered plant mixed extract, taking it out, and adding 60 to 80% (v/ v) preparing a ginseng extract by adding alcohol 7 to 9 times (v/w), extracting at 55 to 60° C. for 1 to 3 hours, and then filtering; and
(2) When the ginseng extract prepared in step (1) is supplied to the lower part of the plate-type evaporator at 50 to 60° C. in which a plurality of plates are stacked, steam is generated from the extract due to the steam supplied from the upper part and is converted to steam. Discharged, steam is condensed by supply of cooling water and discharged in the form of condensed water, and the extract remaining after being discharged as steam is a method for producing a ginseng concentrate, comprising the step of concentrating to 67 to 71 brix.
제4항에 있어서, 상기 (2)단계의 농축한 인삼 농축액을 85~95℃에서 5~7시간 동안 열처리하는 것을 추가로 포함하는 것을 특징으로 하는 인삼 농축액의 제조방법.The method of claim 4, further comprising heat-treating the concentrated ginseng concentrate in step (2) at 85 to 95° C. for 5 to 7 hours. 제1항에 있어서,
(1) 흑삼 혼합물 총 중량 기준으로, 흑삼 48~52 중량%, 마타리 뿌리 18~22 중량%, 여뀌잎 18~22 중량% 및 고마리잎 8~12 중량%를 혼합한 흑삼 혼합물에 물을 7~9배(v/w) 첨가하여 80~90℃에서 1~3시간 동안 추출한 후 여과한 식물 혼합 추출물에 인삼을 45~55℃에서 2~4시간 동안 침지한 후 꺼내고 분쇄한 인삼 분쇄물에 60~80%(v/v) 주정을 7~9배(v/w) 첨가하여 55~60℃에서 1~3시간 동안 추출한 후 여과하여 인삼 추출액을 제조하는 단계; 및
(2) 다수의 플레이트가 적층되어 있는 50~60℃의 판형증발농축기의 하부에 상기 (1)단계의 제조한 인삼 추출액을 공급하면, 상부에서 공급되는 스팀으로 인해 추출액으로부터 수증기가 발생하여 증기로 배출되고, 스팀은 냉각수의 공급으로 응축되어 응축수 형태로 배출되고, 증기로 배출되고 남은 추출액은 67~71 brix로 농축하는 단계를 포함하여 제조하는 것을 특징으로 하는 인삼 농축액의 제조방법.
According to claim 1,
(1) Based on the total weight of the black ginseng mixture, water 7~ Add 9 times (v/w), extract at 80~90℃ for 1~3 hours, immerse ginseng in the filtered plant mixed extract for 2~4 hours at 45~55℃, take it out and add 60 to the pulverized ginseng powder Preparing a ginseng extract by adding ~80% (v/v) alcohol 7 to 9 times (v/w), extracting it at 55 to 60° C. for 1 to 3 hours, and then filtering; and
(2) When the ginseng extract prepared in step (1) is supplied to the lower part of the plate-type evaporator at 50 to 60° C. in which a plurality of plates are stacked, steam is generated from the extract due to the steam supplied from the upper part and is converted to steam. Discharged, steam is condensed by supply of cooling water and discharged in the form of condensed water, and the extract remaining after being discharged as steam is a method for producing a ginseng concentrate, comprising the step of concentrating to 67 to 71 brix.
제6항에 있어서, 상기 (2)단계의 농축한 인삼 농축액을 85~95℃에서 5~7시간 동안 열처리하는 것을 추가로 포함하는 것을 특징으로 하는 인삼 농축액의 제조방법.The method for producing a ginseng concentrate according to claim 6, further comprising heat-treating the concentrated ginseng concentrate in step (2) at 85-95° C. for 5-7 hours. 제1항, 제2항, 제4항 내지 제7항 중 어느 한 항의 방법으로 제조된 인삼 농축액.A ginseng concentrate prepared by the method of any one of claims 1, 2, and 4 to 7.
KR1020220017913A 2022-02-11 2022-02-11 Method for producing ginseng concentrate using plate evaporation concentration technology KR102411104B1 (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR920000256A (en) * 1990-06-30 1992-01-29 이승동 Extraction Method of Ginseng Extract
KR20080089059A (en) * 2007-03-31 2008-10-06 한윤정 An extraction method of ginseng ingredients and ginseng liquor having ingredients manufactured by using the same
KR100881634B1 (en) * 2008-07-10 2009-02-04 주식회사 바이럼 The manufacturing process of extrusion puffed, supersonics waves and fermented red ginseng and mountain ginseng extracts
KR101262764B1 (en) * 2012-09-13 2013-05-09 최성근 New process technology for effective manufacture of red-ginseng extraction and concentration

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR920000256A (en) * 1990-06-30 1992-01-29 이승동 Extraction Method of Ginseng Extract
KR20080089059A (en) * 2007-03-31 2008-10-06 한윤정 An extraction method of ginseng ingredients and ginseng liquor having ingredients manufactured by using the same
KR100881634B1 (en) * 2008-07-10 2009-02-04 주식회사 바이럼 The manufacturing process of extrusion puffed, supersonics waves and fermented red ginseng and mountain ginseng extracts
KR101262764B1 (en) * 2012-09-13 2013-05-09 최성근 New process technology for effective manufacture of red-ginseng extraction and concentration

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