KR102105111B1 - Energy drink composition containing increased biological active materials and preparation method thereof - Google Patents

Abstract

The present invention comprises: an energy drink composition with enhanced functionality of antioxidant activity and anti-diabetic activity by increased content of physiologically active components such as a flavonol compound, a phenolic acid compound, GABA, etc.; and a manufacturing method thereof. The energy drink composition according to the present invention has effects of rapidly supplying energy, awakening, and recovering sports ability, and also has antioxidant activity by having remarkably increased physiologically active components such as the flavonol compound, the phenolic acid compound, GABA, etc., thereby having a function of fatigue recovery through radical eradication generated by oxidative stress. In addition, the energy drink composition has enhanced anti-diabetic activity.

Description

Energy drink composition with enhanced bioactive ingredients and manufacturing method thereof {Energy drink composition containing increased biological active materials and preparation method thereof}

The present invention relates to an energy drink composition with enhanced bioactive ingredients and a method for manufacturing the same, more specifically, a flavonol compound, a phenolic acid compound, a gaba, etc., including a Yeoju extract, an active ginseng extract, an Omija sugar extract, and a green tea extract It relates to an energy drink composition and a method of manufacturing the enhanced functionality of the antioxidant activity and anti-diabetic activity by increasing the content of the bioactive component of

Energy drinks are drinks for replenishing energy, and if sports drinks are mainly used for the purpose of replenishing moisture and electrolytes, energy drinks are mainly drinks containing ingredients that can increase energy. It is especially popular with consumers who enjoy extreme sports or young people with high physical and brain activities. Energy drinks are often added with guarana extracts containing simple sugars and caffeine with high calories for the purpose of boosting the body's metabolism and revitalizing them, and amino acids and vitamins such as taurine for fatigue recovery and prevention of muscle damage Is also added.

The guarana ( Paullinia cupana ) is a vine plant of the genus Aptaceae, native to the Brazilian basin. In particular, Guarana seeds are known to be effective stimulants as dietary supplements because they contain very high concentrations of caffeine. Guarana's caffeine contains about twice as much as coffee beans, so the guarana extract is often used as an additive or stimulant in energy drinks (Patent Publication 10-2018-0059095).

Taurine was first isolated from the bull's bile in 1827, and the name of the taurine is a type of amino acid derived from the Latin 'taurus', meaning bull. Taurine is rarely found in plants, but in major human organs. It has been found that the physiological activity of taurine has an inhibitory effect on the sympathetic nerves of the brain, and is also effective in preventing various vascular diseases by suppressing low-density lipoprotein (LDL) cholesterol production. In particular, taurine biosynthesis is extremely limited in these animals because the cysteine sulfinate decarboxylase activity, which is an essential element in the taurine biosynthesis process, is very low in humans and monkeys compared to rats and dogs. Taurine has been used as an ingredient in energy drinks because it has the ability to improve athletic performance and relieve anxiety.

Oxidative stress refers to a state in which the balance of the bio-oxidation, which was maintained almost constant by the balance of the free radical production system and the scavenging system, is broken by various factors such as drugs, radiation, ischemia, and aging. Atherosclerosis, degenerative disease by generating excessive free radicals, breaking the balance of the antioxidant mechanism of the human body, resulting in cellular injury (especially cell membrane lipids and oxidation), mutation by DNA base modification, and induction of cell death (apotosis) Brain diseases, paralysis, Parkinson's disease, Huntington's disease, diabetes, cancer, Alzheimer's, dementia, aging, etc. are causing various diseases. This oxidative stress can be alleviated by the elimination of generated radicals.

Alpha-glucosidase (glucosidase) is an enzyme present in the mucous membrane of the small intestine and is essential for digestion and absorption because it breaks down carbohydrates into monosaccharides of glucose units when food is consumed. Inhibition of these enzymes can inhibit the degradation of carbohydrates, resulting in inhibition of glucose uptake and reduction of blood glucose, and thus agents having inhibitory activity of these enzymes are used as therapeutic agents for diabetes.

Conventional energy drinks are tailored to rapid energy supply based on caffeine and purified amino acids, and thus have a problem of lacking a function of original fatigue recovery through radical scavenging caused by oxidative stress.

The object of the present invention is to increase the content of flavonol compounds, phenolic acid compounds and GABA (γ-aminobutyric acid), including Yeoju extract, active ginseng extract, omija sugar extract, and green tea extract, thereby improving antioxidant activity and anti-diabetic activity. It is to provide an energy drink composition with enhanced functionality.

Another object of the present invention is to provide a method for preparing an energy drink composition having enhanced functionality of antioxidant activity and anti-diabetic activity by increasing the content of flavonol compound, phenolic acid compound, and gaba.

In order to achieve the above object, the present invention provides an energy drink composition comprising a Yeoju extract, an active ginseng extract, an Omija sugar extract, a green tea extract, a guarana extract, taurine and water.

Yeoju extract

The energy drink composition of the present invention contains the Yeoju extract as an active ingredient.

Yeoju ( Momordica charantia L.) is a fruit characterized by a moderate bitter taste, which contains a large amount of phytosterol glycosides and various amino acids such as citrulline, galactonic acid, pectin, and caranthin. . However, Yeoju has a strong bitter taste, so there is a limit to using it as a material for drinks, and it has never been used for energy drinks.

The Yeoju extract is prepared by extracting the Yeoju powder produced by diluting Yeoju with 10-20 times water, extracting it at 120 ° C for 8 hours, filtering it, and extracting it at a concentration of 1 Brix.

In the energy drink composition of the present invention, the extract of Yeoju is contained in 45 to 55% by weight, and if it is less than 45% by weight, the physiologically active ingredient derived from Yeoju is not sufficient and if it exceeds 55% by weight, palatability due to bitterness of the bitter may not be good. .

Active Mountain Ginseng  Extract

The energy drink composition of the present invention includes an active wild ginseng extract as an active ingredient.

In the present invention, mountain ginseng means hemp grown artificially by sowing and transplanting seeds or seedlings under a mountain forest. Goyangsam is known to be more effective than cultivated ginseng in terms of efficacy, and oriental medicine reports that ginseng ginseng has the highest efficacy after natural ginseng, and has higher ginsenoside content in the form of ginseng saponin when compared to cultivated ginseng.

In the present invention, 'active goat ginseng' is produced by repeating steaming and high-temperature aging three times, and refers to processing of wild ginseng so that the content of physiologically active ingredients is enhanced.

In the present invention, 'steaming' means steaming with water vapor at 100 ° C for 30 to 60 minutes. If the steaming time is less than 30 minutes, sufficient steaming does not proceed, so that subsequent steps such as aging may not be smooth and contamination of germs may occur. Can be destroyed.

In the present invention, 'high temperature ripening' means maintaining at 70 to 90 ° C for 2 to 4 days. When the aging temperature is less than 70 ℃ or the aging period is less than 2 days, aging is not smoothly performed. If the aging temperature exceeds 90 ℃ or the aging period exceeds 4 days, the produced bioactive substances may decompose and the content may be reduced. .

In the present invention, the 'active red ginseng extract' is dried for 2 to 3 days at 50 to 55 ° C, and then powdered to 100 mesh or less, and then 10 to 20 times (v / w) active red ginseng powder prepared as described above. ) It can be prepared by extracting at 120 ° C. for 8 to 10 hours by hydrolysis and then filtering to concentrate to a solid content of 4% (4 brix).

In the energy drink composition of the present invention, the active wild ginseng extract is included in 1 to 5% by weight, but if it is less than 1% by weight, it is difficult to expect the physiological activity caused by the wild ginseng, and if it exceeds 5% by weight, a problem of cost increase may occur during beverage production. .

Omija sugar  Extract

The energy drink composition of the present invention contains the extract of Schisandra chinensis as an active ingredient.

Omija (chizandra chinensis BAALL) is called omija because it has five flavors: sweet, salty, sour, bitter, and spicy. Omija contains lignan-type compounds such as schizandrin, schizndran, and gomisin, and pharmacological components such as pigments, so it has excellent efficacy in treating asthma and brightens eyes, It has various physiological activities such as detoxifying alcohol, lowering cholesterol, alleviating hyperlipidemia, anti-cancer and anti-tumor.

In the present invention, 'Omija sugar extract' is prepared by mixing Omizawa sugar in a 1: 1 weight ratio, pickling for 50 to 60 days, and filtering only the liquid to obtain a concentration of 60 Brix.

In the energy drink composition of the present invention, the extract of Schisandra chinensis is contained in 4 to 12% by weight, but if it is less than 4% by weight, the content of sugar is low, and thus the effect cannot be sufficiently achieved as an energy drink. Can occur.

Green Tea Extract

The energy drink composition of the present invention contains green tea extract as an active ingredient.

The green tea extract is prepared by extracting green tea powder at a water concentration of 10 to 20 times, extracting it at 120 ° C. for 8 hours, and extracting it at a concentration of 2 Briggs.

In the energy drink composition of the present invention, the green tea extract is contained in 1 to 5% by weight, and if it is less than 1% by weight, the content of physiologically active substances is not sufficient in addition to caffeine, and if it exceeds 5% by weight, palatability may occur due to the astringent taste of green tea. You can.

Guarana extract

The energy drink composition of the present invention comprises a guarana extract at 0.8 to 1.4% by weight.

Guarana extract in the present invention can be used as a powder or liquid extract, it can be easily obtained commercially.

In the energy drink composition of the present invention, if the guarana extract is less than 0.8% by weight, caffeine content, which is the main component of the energy drink, is low, and if it is more than 1.4% by weight, caffeine content is excessive, which may cause caffeine side effects.

Taurine

The energy drink composition of the present invention contains taurine in 0.2 to 2.0% by weight.

Taurine is readily available commercially.

If the taurine in the energy drink composition of the present invention is less than 0.2% by weight, the main ingredient of the beverage is not sufficient, and if it is more than 2.0% by weight, palatability and side effects may be caused by excessive taurine.

If necessary, the energy drink composition of the present invention may further include additives such as vitamins, sweeteners (stevia, aspartame, etc.), fruit concentrates (apples, blueberries, pears, strawberries, grapes, peaches, etc.), herbal medicine extracts, etc. have.

The energy drink composition according to the present invention contains caffeine 2-3 mg / 100ml, taurine 186 mg / 100ml or more, and reducing sugar 26 to 60 mg / ml, thereby providing rapid energy supply, awakening and exercise recovery, and also a flavonol compound 329 Contains ㎍ / ml or more, 72 µg / ml or more of phenolic acid compounds, and 1 mg / 100 ml or more of gaba, so it has enhanced antioxidant activity and has the function of fundamental fatigue recovery through radical scavenging caused by oxidative stress. Has anti-diabetic activity (Table 2 ~ Table 4, Figure 3).

In addition, the energy drink composition of the present invention is excellent in palatability because the taste and aroma of the active ingredients are well harmonized with each other.

According to another object of the present invention, the present invention

Iv) preparing a Yeoju extract with a concentration of 1 Brix by adding 10 ~ 20 times water to the Yeoju powder and extracting it for 8 hours at 120 ℃;

Ii) Steamed wild ginseng at 100 ℃ for 30 ~ 60 minutes, and steamed wild ginseng was repeated three times for 2-4 days at 70-90 ℃ to prepare active ginseng, and then dried at 50-55 ℃ for 2-3 days After making the powder to 100 mesh or less, and 10 to 20 times (v / w) hydrolyzed to extract for 8 to 10 hours at 120 ℃ filtered to prepare an active wild ginseng extract of 4 Brix concentration;

Iv) preparing Omija sugar extract of 60 Brix concentration by mixing Omizawa sugar in a 1: 1 weight ratio and pickling the sugar for 50-60 days and filtering only the liquid;

Iv) preparing a green tea extract having a concentration of 2 Brix by filtering the green tea powder with water 10 to 20 times and extracting it at 120 ° C. for 8 hours;

Ⅴ) Yeoju extract 45 ~ 55% by weight, Active wild ginseng extract 1 ~ 5% by weight, Omija sugar extract 4 ~ 12% by weight, Green tea extract 1 ~ 5% by weight, Guarana extract 0.8 ~ 1.4% by weight, Taurine 0.2 ~ 0.3% by weight And mixing the rest of the water.

If necessary, the method for preparing the energy drink composition of the present invention includes vitamins, sweeteners (stevia, aspartame, etc.), fruit concentrates (apples, blueberries, pears, strawberries, grapes, peaches, etc.), herbal extracts, etc. Additives may be further included to mix.

 The energy drink composition prepared by the production method of the present invention has enhanced flavonol compounds, phenolic acid compounds and gaba contents, thereby enhancing the functionality of antioxidant activity and anti-diabetic activity.

The energy drink composition according to the present invention contains reducing sugar, taurine, and caffeine to have a rapid energy supply, wakefulness, and exercise recovery effect, and also improves antioxidant activity by remarkably enhancing the physiologically active ingredients such as the flavonol compound, phenolic acid compound, and gaba As a result, it has a function of original fatigue recovery through radical scavenging caused by oxidative stress, and also has enhanced anti-diabetic activity.

Due to this enhanced activity, the energy drink composition of the present invention is useful for restoring motor power and improving drowsiness or attention deficit, hyperactivity disorder, narcolepsy due to the excellent stimulant effect, and has enhanced antioxidant activity and anti-diabetic effect, thereby improving fatigue recovery health It is a functional beverage.

In addition, the energy drink composition of the present invention is excellent in palatability because the taste and aroma of the active ingredients are well harmonized with each other.

1 is a photograph of an energy drink composition prepared according to the present invention.
2 is a graph showing the total phenolics and total flavonoids content of the energy drink composition of the present invention. Figure 2a shows the total phenolics content, Figure 2b shows the total flavonoids content.
3 is a graph showing the antioxidant activity of the energy drink composition of the present invention. FIG. 3A shows DPPH radical scavenging activity, FIG. 3B shows ABTS radical scavenging activity, FIG. 3C shows hydroxyl radical scavenging activity, and FIG. 3D shows FRAP reducing power.
4 is a graph showing the alpha-glucosidase inhibitory activity of the energy drink composition of the present invention.

The invention is explained in more detail by the following examples. These examples are intended to illustrate the invention, and the scope of the invention should not be limited by them.

Manufacturing example  1. Preparation of energy drink composition

<Preparation of Yeoju extract>

50 g of dried Yeoju powder and 0.5 L of purified water were added to the extraction container, and then extracted at 120 ° C. for 8 hours, filtered through a strainer to prepare a Yeoju extract (1 Brix).

<Preparation of active wild ginseng extract>

After washing 3 times in flowing water of 1 kg of wild ginseng for 3 years or more and completely draining the water, boil it at 100 ℃ for 1 hour in a steamer, put it on a dry plate and let it evaporate for 3 days at 75 ℃ (steaming) And high-temperature aging) 3 times to prepare active ginseng and then dry it with hot air at 55 ° C for 3 days, pulverize it with a grinder, powder 50 g into an extraction container and add 10 times purified water (0.5 L) And extracted at 120 ℃ for 8 hours to prepare a wild ginseng extract (4 brix).

<Omija sugar extract preparation>

500 g of Omija and 500 g of sugar were mixed and pickled for 60 days, and then the liquid was filtered to prepare an Omija sugar extract.

<Production of green tea extract>

50 g of dry green tea powder and 0.5 L of purified water were added to the extraction container, and then extracted at 120 ° C. for 8 hours, filtered through a strainer to prepare green tea extract (2 brix).

Guarana extract powder, taurine, additives stevia and vitamin C were purchased and used.

An energy drink composition was prepared by mixing Yeoju extract, active ginseng extract, Omija sugar extract, green tea extract, Guarana extract, taurine, additives (Stevia, Vitamin C) and water prepared in the above manner at the mixing ratio shown in Table 1 below. Examples 1-4).

For comparison, a beverage composition containing only Yeoju extract as an active ingredient was prepared (Comparative Example)

Beverage composition (g) Comparative example Example 1 Example 2 Example 3 Example 4 Goat ginseng extract (brix 4%) - 5 5 5 5 Yeoju extract (brix 1%) 250 250 250 250 250 Omija sugar extract
(brix 60%) - 25 50 25 50 Guarana Extract Powder
(Caffeine 30 mg / g) - 4 7 7 4 Taurine (1000 mg / g) - One One One One Stevia - 0.5 0.5 0.5 0.5 Vitamin C - 0.25 0.25 0.25 0.25 Green Tea Extract (brix 2%) - 5 5 5 5 Purified water 250 209.25 181.25 206.25 184.25 Sum 500 500 500 500 500

Test example  1. Analysis of physicochemical properties

The physicochemical properties of the beverage compositions of Examples and Comparative Examples prepared above were analyzed by measuring pH, acidity, soluble solids and reducing sugar content.

The pH was measured using a pH meter, and the acidity was reverse titrated according to the consumption amount of 0.1 N NaOH through the neutralization titration method and expressed as the final lactic acid (%). Soluble solids were measured using a sugar meter. The reducing sugar content was measured according to the DNS (dinitrosalicylic acid) method, by adding 3 ml of a DNS reagent to 1 ml of the composition of each of the comparative examples and examples diluted appropriately, and boil-cool to measure the absorbance at 550 nm using a spectrophotometer and prepare in advance. It was calculated as mg by substituting the calibration curve for the standard product (glucose), and the results are shown in Table 2.

Analysis items Comparative example Example 1 Example 2 Example 3 Example 4 pH 5.12 3.72 3.73 3.81 3.70 Acidity (%, lactic acid) 0.1 0.34 0.54 0.36 0.62 Soluble solids
(Brix,%) 0.30 5.0 8.70 5.70 8.30 Reducing sugar (mg / ml) 0.32 26.69 56.23 34.38 54.33

As shown in Table 2, Examples 1 to 4 have an average pH of 3.74 and an acid value of 0.34 to 0.62, which is suitable as an energy drink, so that the soluble solid content is 5 to 8.7 Brix and the reducing sugar content is 26.69 to 56.23 mg / ml, providing rapid energy. It is suitably contained in.

Test example  2. Analysis of bioactive components

The taurine and caffeine content of the beverage compositions of the examples and comparative examples prepared above were measured, and the contents of the bioactive components of the gaba, flavonol compound and phenolic acid compound were analyzed.

Taurine and gaba content analysis was performed using an automatic amino acid analyzer, and content analysis of caffeine, flavonol compound, and phenolic acid compound was performed using high-performance liquid chromatography (HPLC).

<Analysis of Taurine and Gaba>

For taurine and gaba content analysis, 1 ml of each example and comparative example composition was accurately weighed in a test tube, 4 ml of HPLC grade water was added, and a hydrolysis process was performed at 60 ° C for 1 hour. After hydrolysis, 2 ml of 5-sulfosalicylic acid was added, the protein was precipitated for 2 hours, and only the supernatant was completely evaporated at 60 ° C. After the complete evaporation, the remaining filtrate was dissolved by adding 2 ml of lithium citrate buffer (pH 2.2), and 2 ml of 5-sulfosalicylic acid was added and left to stand for 2 hours to precipitate the protein. It was evaporated completely under reduced pressure at ℃. Thereafter, 2 ml of lithium citrate buffer (pH 2.2) was added to the remaining filtrate to dissolve and filtered through a 0.45 μm membrane filter was injected into an automatic amino acid analyzer to perform analysis based on a series of methods. Showed.

Content (mg / 100 mL) ¹⁾ Comparative example Example 1 Example 2 Example 3 Example 4 Taurine nd ^{2 )} 188.12 192.33 186.94 190.12 Gaba (γ-aminobutyric acid) 0.51 2.72 8.22 6.37 1.03 Caffeine nd 2.02 2.93 2.95 2.04 ¹⁾ All experiments were performed three times and expressed as an average value.
²⁾ nd: not detected.

<Caffeine, flavonol compound and phenolic acid compound analysis>

The first filtration of the compositions of each Example and Comparative Example with a 0.45 μm filter was prepared as an HPLC analysis sample.

The column used for the analysis was X-Bridge ^TM C18 (4.6 × 250 mm, 5 μm, Waters, USA), and the analysis solvent was 0.2% water containing glacial acetic acid (mobile phase A) and 0.2% water containing glacial acetic acid aceto Prepared with nitrile (mobile phase B) and solvent conditions were 15%, 5%, 15%, 5% for 10, 15, 20, 25, 30, 35, 40, 45, 55 and 60 minutes, respectively, based on mobile phase B. , 10%, 50%, 50%, 60%, 80% and 90%. 20 µl of each prepared analytical sample was injected, and the speed of the mobile phase was maintained at 1 ml / min at 30 ° C. Caffeine, flavonol, and phenolic acid compounds absorbed 280 nm (wavelength measured by the phenolic acid compound) of the diode array UV detector and 270 It was quantified by measuring at nm (caffeine, flavonol compound measurement wavelength), and the results are shown in Tables 3 and 4.

Content (㎍ / mL) ¹⁾ Comparative example Example 1 Example 2 Example 3 Example 4 Flavonol compounds Epigallocatechin 35.87 84.95 74.78 79.18 68.38 Catechin 25.19 33.74 50.53 49.23 61.35 Epicatechin 4.24 16.85 25.47 24.73 18.51 Epigallocatechin gallate 2.52 8.95 12.52 11.72 11.93 Rutin 2.78 8.01 12.18 10.99 10.84 Catechin gallate 2.89 6.21 7.98 7.45 7.23 Quercetin 132.25 163.28 192.47 181.18 190.69 Naringin 1.01 3.97 7.38 4.93 4.02 Naringenin 1.14 1.22 1.21 0.18 0.26 Formonoetin 2.22 2.20 2.18 2.15 2.22 Sum 210.11 329.38 386.70 371.74 375.43 Phenolic acid compound Gallic acid 4.0 9.72 11.56 11.79 10.03 Protocatechuic acid nd ^{2 )} 2.38 1.00 1.10 1.02 Chlorgenic acid 22.06 43.86 40.16 41.21 40.73 p-Hydrobenzoic acid 5.26 9.13 13.37 13.21 13.68 Vanillic acid nd 2.03 4.01 3.72 3.37 Veratric acid 1.57 4.74 5.01 4.76 3.51 t-Cinnamic acid 0.16 0.15 0.18 0.11 0.20 Sum 34.20 72.01 75.29 75.90 72.54 ¹⁾ All experiments were performed three times and expressed as an average value.
²⁾ nd: not detected.

As shown in Table 3, the energy drink composition of Examples 1 to 4, taurine is contained in an amount of 188.12 to 192.33 mg / 100ml, caffeine is contained in an amount of 2.02 to 2.95 mg / 100ml, and is suitable as an energy drink. .

Energy drink composition according to the present invention can be confirmed that the gaba content is 2-16 times (1.03 ~ 8.22 mg / 100ml) enhanced compared to the comparative example (0.51 mg / 100ml).

In addition, as shown in Table 4, the energy drink composition of Examples 1 to 4, compared to the beverage composition of the comparative example, flavonol compounds (epigallocatechin, catechin, epicatechin, epigallocatechin gallate, rutin, catechin gallate, quercetin, naringin, naringenin, formonoetin) and phenolic acid compounds (gallic acid, protocatechuic acid, chlorgenic acid, p-hydrobenzoic acid, vanillic acid, veratric acid, t-cinnamic acid). In particular, it can be confirmed that the energy drink composition of the present invention contains an epicatechin of 16.85 μm / ml or more and a chlorogenic acid of 40.16 μm / ml or more.

From the above results, it can be seen that the energy drink composition of the present invention has a significantly enhanced bioactive component.

Test example  3. Water soluble Phenolic  And Flavonoids  Content analysis

The total phenolics and flavonoids contents of the beverage compositions of Examples and Comparative Examples prepared above were compared and analyzed by Folin-Denis and Davis method.

<Water soluble phenolics content>

The water-soluble phenolics content was measured by slightly modifying the Folin Denis method (1912).

Specifically, 0.5 mL of each Example and Comparative Example composition was dispensed into test tubes, and 0.5 ml of a 25% Na ₂ CO ₃ solution was added thereto, and allowed to stand for 3 minutes. To this, 0.25 ml of 2N-Folin-Ciocalteu phenol reagent was added and mixed, and then allowed to stand at 30 ° C. for 1 hour, and absorbance was measured at 750 nm using a spectrophotometer. At this time, the content of the water-soluble phenolics was obtained from a standard calibration curve prepared in advance (Gallic acid), and the results are shown in FIG. 2A.

As shown in Figure 2a, the water-soluble phenolics content was significantly improved in the energy drink compositions of Examples 1 to 4, compared to the comparative example.

<Flavonoids content>

 After adding 0.01 ml of 1 N NaOH to 0.1 ml of each Example and Comparative Example composition, 1.0 ml of diethylene glycol was added thereto, and after standing at 37 ° C. for 1 hour, absorbance at 420 nm was measured. At this time, the content of flavonoids was obtained from the standard (routine, rutin) calibration curve, and the amount corresponding to rutin was calculated in mg / ml and is shown in FIG. 2B.

As shown in Figure 2b, the flavonoids content, when compared to the comparative example, the energy drink composition of Examples 1-4 was significantly improved.

Test example  4. Antioxidant activity analysis

Antioxidant activity was measured by measuring DPPH radical scavenging activity, ABTS radical scavenging activity, hydroxyl radical scavenging activity and FRAP reducing power.

<DPPH radical scavenging activity>

To 0.1 ml of the composition of each Example and Comparative Example, 0.4 ml of DPPH solution (1.5 × 10 ⁻⁴ M) was added and left to stand for 30 minutes after uniformly mixing, and the absorbance was measured at 525 nm. The negative control of DPPH radical scavenging activity was performed in the same manner using distilled water instead of the composition of each example, and the difference in absorbance was calculated as a percentage (%) by the following equation, and the results are shown in FIG. 3A.

Radical scavenging activity (%) = [1- (absorbance of negative control ÷ absorbance of experimental sphere)] × 100

<ABTS radical scavenging activity>

For ABTS radical scavenging activity, 5 ml of 7 mM ABTS reagent and 5 ml of 140 mM K ₂ S ₂ O ₈ (FW 270.3, Sigma 9392) were mixed and left in the dark for 16 hours to generate cationic radicals, followed by mixing with methanol to mix with 732 nm. The ABTS solution adjusted so that the absorbance value of the control was 0.7 ± 0.02 was used.

 0.1 ml of the composition of each Example and Comparative Example and 0.9 ml of ABTS solution were mixed for 3 minutes, and absorbance was measured at 732 nm. The ABTS radical inhibitory activity was also performed in the same way by substituting distilled water for each composition, and the difference in absorbance was calculated as a percentage (%) by the above formula, and the results are shown in FIG. 3B.

<Hydroxyl radical scavenging activity>

Hydroxy (· OH) radical scavenging activity is FeSO _{4 .} 2 ml of 7H ₂ 0-EDTA (10 mM) and 0.2 ml of 2-deoxyribose (10 mM), 0.2 ml of H ₂ O ₂ (10 mM), 1.4 ml of the composition of each Example and Comparative Example, and mixed at 37 ° C. After reacting for 4 hours, 1 ml of 1% TBA (thiobarbituric acid) and 2.8% trichloroaceric acid (TCA) were added to the mixture, followed by color development at 100 ° C. for 20 minutes, followed by cooling by measuring absorbance at 520 nm. The radical scavenging activity was calculated as the percentage (%) of the difference in absorbance between the addition and non-addition of the sample solution, and the results are shown in FIG. 3C.

<FRAP reducing power analysis>

FRAP (Ferric reducing antioxidant power) is a method of measuring the reducing power of a compound. In more detail, when the Fe3-TPTZ (ferric tripyridyl triazine) is reduced to blue Fe2-TPTZ (ferrous tripyridyl triazine) by the reducing power of the sample, the absorbance is measured to determine the antioxidant activity.

As a reactant in FRAP reducing power measurement, 30 mM acetate buffer (pH 3.6), 10 mM 2,4,6-tripyridyl-s-triazine dissolved in 40 mM hydrochloric acid (TPTZ, T1253, C18H12N6, MW312.33) and 20 mM FeCl ₃ (F7134, MW 162.20, in DW) was used, and the acetate buffer, TPTZ solution and FeCl ₃ solution were mixed at 10: 1: 1 (v / v / v) to pre-react at 37 ° C for 15 minutes. Ordered.

After dispensing 0.05 ml of each Example and Comparative Example composition prepared above and 0.95 ml of FRAP reagent into a test tube, reacting for about 15 minutes and measuring absorbance at 593 nm, the results are shown in FIG. 3D.

3A to 3D, when compared with the beverage composition of the comparative example, the energy beverage compositions of Examples 1 to 4 according to the present invention had a significantly enhanced DPPH radical scavenging activity on average of 37.31%, and ABTS radical scavenging activity , It can be seen that hydroxyl radical scavenging activity and FRAP reducing power are also significantly improved. In particular, the energy drink composition of Example 2 was ABTS radical scavenging activity was 60%, hydroxyl radical scavenging activity was 53%, FRAP reducing power was 1.89, which was very excellent in antioxidant activity.

Test example  5. Anti-diabetes  Active analysis

The antidiabetic activity of the beverage compositions of Examples and Comparative Examples prepared above was performed using a model based on enzymes (α-glucosidase).

1.0 ml of each Example and Comparative Example composition prepared above, 1.0 ml of enzyme solution (α-glucosidase, 1.0 unit / ml), and 1.0 ml of sodium phosphate buffer solution (pH 6.8, 200 mM) were dispensed into a test tube and 37 ℃ Preliminary reaction was performed in the water bath for 15 minutes. After completion of the pre-reaction, 0.5 ml of para-nitrophenyl glycogen (5 mM) dissolved in a sodium phosphate buffer solution was added to perform a final enzymatic reaction in a water bath at 37 ° C. After completion of the enzymatic reaction, 0.75 ml of sodium carbonate (100 mM) was added to the reaction product to terminate the reaction, and absorbance was measured at 420 nm. At this time, the negative control was replaced with distilled water instead of each composition, and substituted into the same formula as the radical scavenging activity. The inhibitory activity was calculated by the final%, and the results are shown in FIG. 4.

As shown in FIG. 4, the energy drink compositions of Examples 1 to 4 exhibited about 2 to 4 fold (7 to 13.44%) enhanced α-glucosidase inhibitory activity compared to Comparative Example (3%). . From these results, it can be seen that the energy drink composition of the present invention has significantly enhanced anti-diabetic activity.

Claims (5)

Yeoju extract 45 ~ 55% by weight, active wild ginseng extract 1 ~ 5% by weight, Omija sugar extract 4 ~ 12% by weight, green tea extract 1 ~ 5% by weight, guarana extract 0.8 ~ 1.4% by weight, taurine 0.2 ~ 2.0% by weight and the rest An energy drink composition comprising water,
The active ginseng extract is prepared by evaporating the wild ginseng at 100 ° C for 30 to 60 minutes, and then evaporating the boiled wild ginseng at 70 to 90 ° C for 2 to 4 days at high temperature three times to prepare the active ginseng, and then at 2 to 50 to 55 ° C. After drying for 3 days, powdered to 100 mesh or less, 10-20 times (v / w) hydrolyzed, extracted at 120 ° C. for 8-10 hours, filtered and prepared at a concentration of 4 Brix,
The beverage composition contains a gaba content enhanced to 1.03 to 8.22 mg / 100 ml, an epicatechin content enhanced to 16.85 to 25.47 μg / ml, and a chlorogenic acid enhanced to 40.16 to 43.86 μg / ml, enhanced to 40-60% Energy drink composition characterized by having an ABTS radical scavenging activity and an α-glucosidase inhibitory activity enhanced to 7-13.44%.
delete delete delete A method for producing an energy drink composition according to claim 1, wherein the method
Iv) preparing a Yeoju extract having a concentration of 1 Brix by adding 10-20 times water to the Yeoju powder and extracting it at 120 ° C for 8 hours;
Ii) Steamed wild ginseng at 100 ℃ for 30 ~ 60 minutes, and steamed wild ginseng was repeated three times for 2-4 days at 70-90 ℃ to prepare active ginseng, and then dried at 50-55 ℃ for 2-3 days After making the powder to 100 mesh or less, and 10 to 20 times (v / w) hydrolyzed to extract for 8 to 10 hours at 120 ℃ filtered to prepare an active wild ginseng extract of 4 Brix concentration;
Iv) preparing Omija sugar extract of 60 Brix concentration by mixing Omizawa sugar in a 1: 1 weight ratio, pickling and filtering for 50-60 days;
Iv) preparing a green tea extract having a concentration of 2 Brix by filtering the green tea powder with water 10 to 20 times and extracting it at 120 ° C. for 8 hours;
Ⅴ) Yeoju extract 45 ~ 55% by weight, Active wild ginseng extract 1 ~ 5% by weight, Omija sugar extract 4 ~ 12% by weight, Green tea extract 1 ~ 5% by weight, Guarana extract 0.8 ~ 1.4% by weight, Taurine 0.2 ~ 2.0% by weight And mixing the rest of the water.

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