KR102007576B1 - Beverage composition of Aronia melanocarpa containing active ginsenosides and preparation method thereof - Google Patents

Abstract

 The present invention discloses a phenolic acid and flavonol enhanced aronia beverage composition containing the active ginsenosides ginsenosides Rd, Rd2, Rg3, F2, and physiologically active ingredients, and a preparation method thereof. Aronia beverages according to the present invention contains the active ginsenosides Rd, Rd2, Rg3, F2 and compound K, and enhance the phenolic acid and flavonol, and also has excellent alpha-glucosidase inhibitory activity and pancreatic lipase inhibitory activity Antioxidant activity, antidiabetic activity and anti-obesity activity are enhanced and can be used as a material for functional food and pharmaceuticals.

Description

Beverage composition of Aronia melanocarpa containing active ginsenosides and preparation method

The present invention relates to an active ginsenoside-containing aronia beverage composition and a method for preparing the same, and more particularly, to an active ginseng concentrate prepared by using a goat goat outpost and undergoing high temperature aging under specific conditions. The present invention relates to a phenolic acid and flavonol-enhanced aronia beverage composition containing sidesides ginsenosides Rd, Rd2, Rg3, F2 and compound k, and physiologically active ingredients.

Panax, a generic name of Korean ginseng, is a compound word of Greek Pan (all) and Akok (to cure). It is a medicinal crop that has been widely used for food and medicine since ancient times. Ginseng consists of about 60% carbohydrates, 8-15% crude protein, 1-3% crude fat, 4-6% ash, 3-7% irradiated ponin, and other minor components. Ginseng has been reported to have various pharmacological effects on the regulation of physical function by affecting the central nervous system, endocrine system, immune system, metabolic system, and such pharmacological effects are known to be due to the origin of active ginsenosides.

Active ginsenosides include ginsenosides Rg1, Rg3, Rd, Rf, Rh, F2, and compound K, which are easily absorbed by the human intestine. Ginsenoside Rg1 improves learning function, anti-fatigue, ginsenoside Rf acts on cranial nerve cell analgesic, inhibits lipid peroxidation, ginsenoside Rg3 inhibits cancer cell metastasis, hepatoprotective action, anticancer drug inhibitory action, ginsenoside Rd Cortical hormone secretion promoting action, ginsenoside compound K has been shown to be anti-cancer action, hepatoprotective action, skin protection action, tumor growth inhibitory action, antioxidant action, anti-allergic action. In particular, ginsenoside compound k (20-O-beta-D-glucopyranosyl-20 (S) -protopanaxadiol) is a ginsenoside metabolite, which is known for its excellent absorption in the body and excellent pharmacological activity. Disease treatment, neurodegenerative pain treatment is also reported (registered patent 10-1539573).

As the preference for ginseng is changing from ginseng to red ginseng and recently, no-pesticide wild ginseng (wild ginseng), the demand for functional foods using wild ginseng is increasing rapidly.

Aronia melanocarpa is a rose family belonging to the family Rosaceae, which has been dominant in Korea in recent years, growing in eastern America in North America, and its production is increasing. Research on the composition of Aaronia has been conducted continuously in the Warsaw University of Poland, and has recently published many research results in Korea.

Aaronia contains vitamins A, C, E, B2, B6, B9, B12, folic acid, quinic acid, phenolic acids, anthocyanin, tannin, and catechins. ), Quercitin, lutein & zeaxanthin, rutin, hesperidin, resveratrol, beta-carotene, calcium, iron, magnesium, zinc, It contains various organic minerals such as potassium and manganese, and the content of particularly powerful antioxidants, anthocyanins and polyphenols, is known to be the highest among berries. Aronia is known to be effective in improving cardiovascular disease, cancer, diabetes, gastritis and allergic skin diseases as well as antioxidant effects. Recently, aronia is widely used as a raw material for beverages. As an example, techniques related to the production of Aaronia beverages prepared by mixing with fruits of high sugar such as grapes and pears have been developed in Aaronia (Patent No. 10-2017-0093087, Patent No. 10- 1772014).

However, there is no known Aaronia beverage composition containing active ginsenosides and a preparation method thereof.

Therefore, the present inventors have continued the research to meet the demands of the prior art, the result is that the Aaronia beverage prepared by adding a goat goat concentrate prepared by using a goat goat outpost and undergoing high temperature aging under specific conditions to the Aaronia juice Ginsenosides containing Rd, Rd2, Rg3, F2 and compound K, and confirmed that the physiologically active phenolic acid and flavonol are significantly enhanced, the present invention was completed.

It is therefore an object of the present invention to provide a process for preparing an Aaronia beverage composition containing an active ginsenoside.

It is another object of the present invention to provide an aronia beverage composition prepared by the above method, containing active ginsenosides and having enhanced phenolic acid and flavonol.

Still another object of the present invention is to provide a functional food having enhanced antioxidant activity, antidiabetic activity and anti-obesity activity, including the Aaronia beverage composition.

In order to achieve the above object, the present invention provides a method for preparing an Aaronia beverage composition containing an active ginsenoside comprising the following steps:

Iii) steaming goat goat outpost at 100 ° C. for 30 to 60 minutes and repeating high temperature aging 3 to 5 times at 70 to 90 ° C. for 2-3 days to produce active goat ginseng;

Ii) powdering active goat ginseng and extracting by adding purified water;

Iii) filtering the active goat ginseng extract and concentrating to 15-25 briquettes;

Iii) mixing 2-10 parts by weight of the active goat ginseng concentrate to 100 parts by weight (v / v) of the aronia juice; And

Iii) adding a subsidiary material to the mixed liquid to prepare a beverage.

If necessary, the production method of the present invention may further comprise the step of iii) sterilizing the beverage.

In the present invention, 'mountain ginseng' refers to ginseng cultivated as pesticide-free without installing an artificial facility such as a light shielding film in the mountains.

In the present invention, 'outpost (全 草)' means the whole, including the root, stem and leaves of hemp.

Step iii): Preparation of Active Goats

Goat ginseng is steamed at 100 ° C. for 30-60 minutes, and high temperature aging is repeated three to five times at 70-90 ° C. to prepare active goat ginseng.

Steaming goat ginseng outpost (steaming process). Goat ginseng in the present invention uses more than three years. Wash goat ginseng three times in running water, remove water and steam. Steaming can be carried out using a steamer such as a conventional steamer. If the steaming time is less than 30 minutes, sufficient steaming does not proceed, so subsequent steps such as ripening may not be smooth and contamination of various bacteria may occur. This can be destroyed, especially the bioactive components of the leaves and stems can be excessively destroyed.

High temperature aging is less than 70 ℃, ripening period is less than 2 days, or less than 3 times of aging, aging is not smooth, aging temperature is above 90 ℃, aging period is more than 3 days or 5 times If exceeded, the produced bioactive substance may be decomposed and the content may be reduced.

The active goat ginseng prepared as described above has a reduced taste of goat ginseng, which improves palatability while significantly increasing the contents of the active ginsenosides Rd, Rd2, Rg3, F2 and compound k (Table 1).

Step ii) Extracting Active Goat

Active ginseng is powdered and extracted by adding purified water.

Powdering is carried out by drying the active goat ginseng 2 to 3 days at 50-55 ℃ to grind to less than 100 mesh.

Extraction is performed by adding 15-25 times the volume (v / w) of purified water to the active goat ginseng powder and extracting it repeatedly for two to three times for 4 to 6 hours at 95-100 ° C. to prepare the active goat ginseng extract.

Step iii): Preparation of Active Goat Ginseng Concentrate

Filter the active goat ginseng extract and concentrate to 15-25 brics.

Filtration is filtered on a 9 mm filter paper, to remove the active goat ginseng powder to remove the active goat ginseng concentrate of a certain quality after extraction.

The filtrate is heated to 70 to 100 ° C. to concentrate the soluble solids to 15 to 25 brics, thereby preparing an active goat ginseng concentrate.

Step iii) Mixing Aaronia Juice with Active Goat Ginseng Concentrate

To 100 parts by weight of Aaronia juice Mix 2 to 10 parts by weight.

Aaronia juicer 1 ~ 2 times (v / w) It is preferable to use purified water after crushing with a blender by adding purified water.

Purified water counteracts the astringent taste of Aaronia and is added as needed to increase the yield of Aronia juice.

To 100 parts by weight of Aaronia juice It is preferable to mix 2 to 10 parts by weight, but the active ginseng-derived active ginsenoside content, which is less than 2 parts by weight, may be insufficient. It can be dark and deteriorate in palatability.

Step iii): Beverage Manufacturing

Substances are added to the mixed solution from step iii) to prepare a beverage.

Fruit concentrates such as strawberries, apples and pears, honey, citric acid and taurine are added to complete the composition of the Aaronia beverage containing the active ginseng.

The addition amount of the subsidiary material can be appropriately determined by those skilled in the art according to the preference, and preferably 2 to 5% (v / v) of the mixed liquid.

Step iii): Sterilization

Sterilizing active goat ginseng-containing Aaronia beverage composition may be further included.

Sterilization can be carried out in the usual manner used for juice drinks, preferably sterilization for 1 to 5 minutes at 90 ~ 115 ℃.

Sterilization can be done with or before filling the beverage into the container. As a container, glass containers, PET containers, pouch containers, ceramics, etc., which are conventional beverage containers, may be used, but are not limited thereto.

Aronia beverage composition prepared by the preparation method of the present invention contains the active ginsenoside compounds ginsenosides Rd, Rd2, Rg3, F2 and compound k, and phenolic acid and flavonol enhanced, excellent antioxidant activity , Antidiabetic and anti-obesity activity (Tables 3-5, Figures 3a-5b).

It is a further object of the present invention to provide a phenolic acid and flavonol enhanced Aaronia beverage composition containing active ginsenosides Rd, Rd2, Rg3, F2 and compound k, prepared by the above process.

Another object of the present invention to provide a functional food (drinks, etc.) having enhanced antioxidant activity, antidiabetic activity and anti-obesity activity, including the Aaronia beverage composition.

The process of the present invention contains the active ginsenosides Rd, Rd2, Rg3, F2 and compound k, and produces phenolic acid and flavonol enhanced Aaronia beverage compositions.

Aronia beverages according to the present invention contains the active ginsenosides Rd, Rd2, Rg3, F2 and compound K, and enhance the phenolic acid and flavonol, and also has excellent alpha-glucosidase inhibitory activity and pancreatic lipase inhibitory activity Antioxidant activity, antidiabetic activity and anti-obesity activity is enhanced and can be used as a material for functional foods.

Functional food according to the present invention has excellent anti-diabetic and anti-obesity functionality, useful for inhibiting fat production, weight control, cholesterol lowering, hyperlipidemia, arteriosclerosis relief, diabetes relief, blood circulation improvement, immunity improvement.

1 is a view showing an example of the manufacturing process of the active goat ginseng-containing Aaronia beverage composition according to the present invention.
Figure 2 shows the ginsenoside HPLC chromatogram of active ginseng-containing Aaronia beverage composition according to the present invention.
Figure 3 shows the bioactive material content of the active goat ginseng-containing Aaronia beverage composition according to the present invention. Figure 3a shows the total phenolics content, Figure 3b shows the total flavonoid content, Figure 3c shows the total anthocyanin content.
Figure 4 shows the antioxidant activity of the active goat ginseng-containing Aaronia beverage composition according to the present invention. FIG. 4A shows DPPH radical scavenging activity, FIG. 4B shows ABTS radical scavenging activity, FIG. 4C shows hydroxyl radical scavenging activity, and FIG. 4D shows FRAP reducing power.
Figure 5 shows the digestive enzyme inhibitory activity of the active goat ginseng-containing Aaronia beverage composition according to the present invention. 5a shows alpha-glucosidase inhibitory activity, and FIG. 5b shows pancreatic-lipase inhibitory activity.

The invention is explained in more detail by the following examples. These examples are intended to illustrate the invention and the scope of the invention should not be limited by them.

Preparation Example 1 Preparation of Active Goat Ginseng Concentrate

Mountain ginseng over 3 years old was grown and used at 500 m above sea level in Seosang-myeon, Hamyang-gun. 10 kg of wild ginseng outposts were washed three times in running water, completely drained, and placed in a steamer. Then, steamed for 30 minutes at 100 ℃ and put the steamed goat ginseng side by side in a dry tray to allow the water to evaporate at a high temperature of 75 ℃ 2 days of aging (steaming and high temperature aging process) was performed four times repeated Goat ginseng was prepared, dried at 55 ° C. for 2 days, and then pulverized to 100 mesh or less using a grinder to powder (active goat ginseng powder). For comparison, goat ginseng outpost was dried for two days at 55 ° C. and then powdered in the same manner (goat ginseng powder).

2 g of purified water was added to 100 g of activated ginseng powder, and the extraction process was repeated twice to obtain an active goat ginseng extract at 100 ° C. for 5 hours. The active goat ginseng extract was filtered using a 9 mm filtration filter, and the filtrate obtained by applying 100 ° C. heat was concentrated to 20 brix so as to prepare an active goat ginseng concentrate. Goat ginseng powder was carried out in the same manner to prepare a concentrate.

Ginsenoside Content Analysis

5 ml each of active goat ginseng concentrate and goat ginseng concentrate were added with the same amount of HPLC water, and the ginsenoside compound content was analyzed and compared using an analytical sample filtered through a 0.45 μm filtration filter.

Ginsenoside analysis was analyzed by high press liquid chromatography (HPLC) with a slight modification of the method described in the functional food assay. As an analytical column, TSKgel ODS-100Z was used for the sample injection amount of 10 μl, the temperature was 30 ° C., the wavelength was 203 nm, the flow rate was 1.0 ml / min. As the mobile phase, HPLC solution A and solution B were acetonitrile. HPLC analysis conditions indicated that mobile phase solution flowed into A solution 81%: B solution 19% at 0 minutes, A solution 80%: B solution 20% at 40 minutes, A solution 77%: B solution 23% at 42 minutes, and 42 minutes A solution 70%: B solution 30%, A solution 65% at 75 minutes: A solution 35%, A solution 30% at 80 minutes: B solution 70%, A solution 10% at 90 minutes: B solution 90% Shed it. The ginsenoside contents of the active goat ginseng concentrate and goat ginseng concentrate are shown in Table 1.

Ginsenoside content ¹⁾
(mg / g dw) Goat Ginseng Concentrate Active Goat Ginseng Concentrate   Ginsenoside Rg1 nd 0.04   Ginsenoside re nd 0.02   Ginsenoside ro 1.44 1.46   Ginsenoside Rf 0.15 0.17   Ginsenoside F3 nd 0.08   Ginsenoside Rg2 0.42 0.52   Ginsenoside Rh1 0.48 0.55   Ginsenoside Rb1 0.46 0.52 Ginsenoside Rd 0.47 0.67 Ginsenoside Rd2 0.14 0.54 Ginsenoside F2 0.78 1.51 Ginsenoside Rg3 0.43 1.01 Ginsenoside Compound K 0.47 1.38   Ginsenoside Rh2 0.05 0.10 Total Ginsenoside 5.29 8.57 ¹⁾ All experiments were repeated three times.
²⁾ nd, not detected.

As shown in Table 1, ginsenosides were 5.29 mg / g 8.57 mg / g, respectively, and the active goat ginseng concentrate according to the present invention showed about 1.6 times higher content than the goat ginseng concentrate. In particular the active ginsenosides ginsenosides Rd, Rd2, F2, Rg3 and compound K (compound K) were enhanced about 2.9-fold.

Preparation Example 2 Preparation of Aronia Beverage Containing Active Goat Ginseng

Aaronia grown in Seosang-myeon, Hamyang-gun was purchased and used. Aronia was washed three times in flowing water 10 kg and completely drained, the same amount of purified water was added, and then pulverized and filtered using a blender to prepare a Aaronia juice.

2 ml of the active goat ginseng concentrate prepared in Preparation Example 1 and 95 ml of aronia fruit juice and 1 ml of strawberry concentrate, 1 ml of honey, 0.5 g of citric acid and 0.5 g of taurine were mixed and placed in a container at 100 ° C. Sterilization for 3 minutes to prepare an Aaronia beverage composition (Example).

A goat drink (Comparative Example) was prepared in the same manner as above by adding a goat goat concentrate.

Physicochemical Characterization

The pH of each beverage prepared above was measured using a pH meter and the total acidity was converted into lactic acid by neutralization titration, and the reducing sugar was converted into glucose using Miller's DNS method, and the soluble solids were refractometer. Table 2 shows the results measured using.

Comparative example Example pH 3.92 3.86 Acidity (%, lactic acid basis) 1.14 1.26 Reducing Sugar (mg / ml) 3.54 3.64 Soluble Solids (Brix) 18.0 21.1

As shown in Table 2, the active goat ginseng-containing Aaronia beverage composition prepared according to the present invention had a slight decrease in pH and a slight increase in total acidity and reducing sugar soluble solids compared to the comparative example. If the pH is high, it is possible to proliferate food microorganisms, etc. If the pH is too low, excessive acid is produced, sour taste is enhanced, which may cause problems in palatability. The active goat ginseng-containing Aaronia beverage composition of the present invention has a suitable acid and sugar, so that the acidity and sweetness are appropriately made.

Test Example 1 Analysis of Active Ginsenoside Content of Aaronia Beverage Composition

The active ginsenoside content of the active goat ginseng-containing Aaronia beverage composition prepared in Preparation Example 2 was analyzed.

Analytical Sample and Ginsenoside Compound Analysis

Each Aaronia beverage (Example, Comparative Example) prepared in Preparation Example 2 was centrifuged at a rate of 3,000 rpm, and only the supernatant was collected and filtered through a 0.45 μm filter, and the filtrate was used as a sample to describe the preparation in Example 1. The ginsenoside content was analyzed as shown, and each HPLC chromatogram is shown in FIG. 2 and the content of the active ginsenoside compound is shown in Table 3.

Ginsenoside content ^{1 )}
(mg / g dw) Comparative example
(Goat Ginseng-containing Aaronia Drink) Example
(Aronia drink containing active wild ginseng)   Ginsenoside Rd nd 0.04   Ginsenoside Rd2 nd 0.03   Ginsenoside F2 nd 0.10   Ginsenoside Rg3 nd 0.09   Ginsenoside Compound K nd 0.09 Total Ginsenoside 0 0.35 ¹⁾ All experiments were repeated three times.
²⁾ nd, not detected.

As shown in Table 3 and Fig. 2, while the active ginseng-containing aronia beverage of the comparative example was not detected at all active ginsenoside, the active ginseng-containing aronia beverage of the example according to the present invention was activated ginsenoside Rd. , Rd2, F2, Rg3 and compound k was found to contain a significant amount.

Test Example 2 Analysis of Biologically Active Compound Content in Aaronia Beverage Composition

The total phenolics, total flavonoids, and total anthocyanin contents of bioactive components of the Aronia beverage composition were analyzed.

<Total Phenolic Content>

Total phenolic content was measured by Folin Denis method (1912).

Specifically, 0.5 ml of each filtrate sample prepared as in Test Example 1 was dispensed into a test tube, and then 0.5 ml of 25% Na ₂ CO ₃ solution was added thereto and allowed to stand for 3 minutes. Then, 0.25 ml of 2N-Folin-Ciocalteu phenolic reagent was added thereto, mixed, and allowed to stand at 30 ° C. for 1 hour, and then absorbance was measured at 750 nm. At this time, the total phenolics content was calculated from the standard curve prepared by using gallic acid to calculate the amount corresponding to gallic acid, and the results are shown in FIG. 3A.

Total Flavonoid Content

Total flavonoid content was determined by the Davis method.

0.01 ml of 1 N NaOH was added to 0.1 ml of each filtrate sample prepared as in Test Example 1, and 1.0 ml of diethylene glycol was added thereto, and the absorbance was measured at 420 nm for 1 hour. In this case, the total flavonoid content was calculated from a standard curve prepared by preparing final concentrations of rutin at 0, 0.25, 0.5, and 1.0 mg / ml, and the results are shown in FIG. 3B.

<Total Anthocyanin Content>

Total anthocyanins were quantified by applying the Lambert-Beer law by the method proposed by Francis (1989).

Each filtrate sample prepared as in Test Example 1 was diluted 10-fold with tertiary distilled water, and after centrifugation, 1 ml of the supernatant was dispensed into a 10 mm thick quartz cell and measured at 530 nm. The anthocyanin content was determined as shown in FIG. 3C:

Total anthocyanate = absorbance (OD530 nm) ÷ 98.2 (absorption coefficient of anthocyanin)

As shown in Figs. 3a to 3c, the total phenolics, total flavonoids and total anthocyanin contents of the Aaronia beverages (containing active goat ginseng) of the examples according to the present invention were higher than those of the comparative example Aaronia beverages (containing goats). (2.72, 13.66 and 2.87 mg / ml, respectively).

Test Example  3. Aaronia  Phenolic Acids in Beverage Compositions Flavonol  analysis

Phenolic acid and flavonols of the Aaronia beverage composition were analyzed.

Phenolic acid and flavonol content analysis was performed using high performance liquid chromatography (HPLC) for each filtrate sample prepared as in Test Example 1.

The analytical column used an XBridge ^™ C18 (4.6 mm, 5 μm, Waters Corp., Milford, MA, USA) column, with 0.5% glacial acetic acid (mobile phase solvent A) and 100% methanol (mobile phase solvent B) Phenolic acid (280 nm) and flavonol (270 nm) were detected by UV detector while operating at a rate of 1 ml per minute for 60 minutes at 30 ° C with a 0-100% linear gradient. Table 5 shows.

Content (μg / ml) ¹⁾ Comparative example
(Goat Ginseng-containing Aaronia Drink) Example (Active Goat Ginseng-containing Aaronia Drink) Gallic acid 10.39 11.37 Protocatechuic acid nd 3.25 Chlorgenic acid 228.24 239.34 p-Hydrobenzoic acid 134.77 151.19 Vanillic acid 14.05 18.03 Ferulic acid 13.33 22.30 ¹⁾ All experiments were repeated three times.
²⁾ nd, not detected.

As shown in Table 4, compared to the comparative example, the Aaronia beverage according to the present invention had a high content of all phenolic acids, in particular protocatecuic acid was newly produced.

Content (μg / ml) ¹⁾ Comparative example
(Goat Ginseng-containing Aaronia Drink) Example (Active Goat Ginseng-containing Aaronia Drink) Epigallocatechin 228.51 231.96 Epicatechin 34.72 49.15 Rutin 79.14 89.55 Quercetin 123.44 149.16 Naringin 67.28 86.92 ¹⁾ All experiments were repeated three times.

As shown in Table 5, compared to the comparative example, the Aronia beverages according to the present invention had a high content of all flavonols, especially epicatechin, quercetin, and the like.

Test Example  4. Aaronia  Antioxidant Activity Analysis of Beverage Composition

OH) 라디칼 소거활성 및 FRAP 환원력을 측정하여 분석하였다. Antioxidant activity of the Aaronia beverage composition according to the present invention is DPPH, ABTS, hydroxyl (

OH) radical scavenging activity and FRAP reducing power were measured and analyzed.

<Analysis sample>

An analytical sample prepared by diluting the stock solution (0 times), 5 times and 10 times volume (v / v) of each filtrate sample prepared as in Test Example 1 was used.

DPPH radical scavenging activity

DPPH radical scavenging activity was added to 0.2 ml of the analytical sample prepared above (three concentrations of each stock solution, 5 times dilution, and 10 times dilution), and 0.8 ml of DPPH solution (1.5 ^-4 M) was added and mixed for 30 minutes. After standing, absorbance was measured at 525 nm. Negative control of DPPH radical scavenging activity was carried out in the same manner using distilled water instead of the sample to calculate the difference in absorbance as a percentage (%) by the following equation, the results are shown in Figure 4a.

% Radical scavenging activity = [1- (negative control absorbance ÷ experimental absorbance)]

ABTS radical scavenging activity

For ABTS radical scavenging activity, 5 ml of 7 mM ABTS reagent and 5 ml of 140 mM K ₂ S ₂ O ₈ (FW 270.3, Sigma 9392) were mixed and left for 16 hours in a dark place to generate a cationic radical. ABTS solution was adjusted so that the absorbance value of the control was 0.7. 0.1 ml of the analyte sample prepared above (three stock solutions, 5 times dilution and 10 times dilution, respectively) and 0.9 ml of ABTS solution were mixed for 3 minutes and absorbance was measured at 732 nm. The negative control of ABTS radical inhibition activity was also proceeded in the same manner using distilled water instead of the sample to calculate the difference in absorbance as a percentage (%) by the above formula, and the results are shown in FIG. 4B.

<Hydroxyl (OH) radical scavenging activity>

The hydroxyl (OH) radical scavenging activity was measured in 10 mM FeSO _{4 . 7} ml of 7H ₂ 0-EDTA, 0.2 ml of 10 mM 2-deoxyribose, 0.2 ml of 10 mM H ₂ O ₂ , 1.4 ml of the assay sample prepared above (stock, 5 times dilution, 3 concentrations of 10 times dilution) 1.4 ml After mixing, the mixture was reacted at 37 ° C. for 4 hours to form a mixed solution. To the mixed solution, 1 ml of 1% thiobarbituric acid / distilled water and 2.8% trichloroaceric acid / distilled water were added to 100 ml. After cooling by developing for 20 minutes at ℃, absorbance was measured at 520 nm. As for the hydroxyl radical scavenging activity, the difference in absorbance between the added and non-added spheres of the sample solution was calculated as a% value by the above formula, and the results are shown in FIG. 4C.

<FRAP reducing power analysis>

Ferric reducing antioxidant power (FRAP) is a method of measuring the reducing power of a compound. Specifically, when the Fe III-TPTZ (ferric tripyridyl triazine) is reduced to blue Fe II-TPTZ (ferrous tripyridyl triazine) by the reducing power of the sample to determine the antioxidant properties by measuring the absorbance.

The reaction solution in FRAP reducing power assay was 30 mM acetate buffer (pH 3.6), 10 mM 2,4,6-tripyridyl-s-triazine (TPTZ, T1253, C18H12N6, MW312.33) dissolved in 40 mM hydrochloric acid, and 20 mM FeCl 3 (F7134, MW 162.20, in DW) was prepared, and the acetate buffer, the TPTZ solution and the FeCl 3 solution were mixed at 10: 1: 1 (v / v / v) and preliminarily reacted at 37 ° C. for 15 minutes. Dispense 50 μl of the sample prepared above (three concentrations of the stock solution, 5 times dilution, and 10 times dilution) and 950 μl of FRAP reagent into a test tube, and react for about 15 minutes using a microplate reader (Biorad 3055, Sweden). The absorbance was measured at 593 nm and the result is shown in FIG. 4D.

As shown in Figures 4a to 4d, the active goat ginseng-containing aronia beverage composition of the embodiment according to the present invention, compared to the comparative example (goats ginseng-containing aronia beverage), scavenging activity and FRAP of DPPH, ABTS, hardoxy radicals Reducing power was significantly enhanced, especially at lower concentrations. In case of 5-fold dilution concentration, DPPH radical scavenging activity was 55.76% in the comparative example and 65.33% in the example, ABTS radical scavenging activity was 79.77% in the comparative example and 96.44% in the example, and hydroxyl radical scavenging activity was 32.19% in the comparative example, The Example was 46.77%, the FRAP reducing power was Comparative Example 1.366 and Example 1.714.

Test Example  5. Aaronia  Digestive enzyme inhibitory activity assay of beverage composition

Alpha-glucosidase inhibitory activity, which is an indicator of antidiabetic (diabetic improvement) effect, was assayed, and pancreatic lipase inhibitory activity, which is an indicator of anti-obesity (improved obesity), was assayed.

Alpha-glucosidase inhibitory activity

To the alpha-glucosidase inhibitory activity, 50 μl of 0.5 U / ml alpha-glucosidase enzyme solution was added to 50 μl of the assay sample prepared in Test Example 4 (three concentrations of the stock solution, 5 times dilution, and 10 times dilution, respectively). 50 μl of 200 mM sodium phosphate buffer (pH 6.8) was mixed and preincubated at 37 ° C. for 10 minutes, and then 100 μl of sodium phosphate buffer (pH 6.8) was added and reacted at 37 ° C. for 10 minutes. 0.75 ml of 100 mM Na ₂ CO ₃ was added to the reaction mixture to stop the reaction, and the absorbance was measured at 420 nm. The negative control proceeded in the same manner using distilled water instead of the sample. ), And the result is shown in FIG. 5A.

Inhibitory activity (%) = [1- (negative control absorbance ÷ experimental absorbance)] × 100

As shown in Figure 5a, the active goat ginseng-containing aronia beverage composition of the embodiment according to the present invention, compared with the comparative example (goat ginseng-containing aronia beverage), the alpha-glucosidase inhibitory activity was significantly enhanced, especially 5 times In the case of dilution concentration, it can be seen that the improvement was about 1.4 times.

Pancreatic-lipase inhibitory activity

Pancreatic-lipase inhibitory activity was measured in 50 μl of the sample prepared in Test Example 4 (three concentrations of the stock solution, 5 times dilution and 10 times dilution, respectively) 50 μl of 1.0 U / ml pancreatic lipase enzyme solution and 200 mM sodium phosphate buffer (pH). 6.8) 50 μl of the mixture was preincubated at 37 ° C. for 10 minutes, and then 100 μl of sodium phosphate buffer (pH 6.8) was added thereto, followed by reaction at 37 ° C. for 10 minutes. 0.75 ml of 100 mM Na ₂ CO ₃ was added to the reaction solution to stop the reaction, and the absorbance was measured at 420 nm. The negative control proceeded in the same manner using distilled water, and the difference in absorbance was expressed by the percentage (%) according to the above formula. It was calculated as, and the result is shown in Figure 5b.

As shown in Figure 5b, the active goat ginseng-containing aronia beverage composition of the embodiment according to the present invention, the pancreatic lipase inhibitory activity was significantly improved compared to the comparative example (goat ginseng-containing aronia beverage) (Comparative example for the stock solution 38.57%, Example 46.22%).

From the above functional assay results, the active goat ginseng-containing aronia beverage according to the present invention contains the active ginsenosides ginsenosides Rd, Rd2, Rg3, F2 and compound k, and not only phenolic acid and flavonol are enhanced. In addition, antioxidant activity, alpha-glucosidase inhibitory activity and pancreatic lipase inhibitory activity is also enhanced, it can be seen that it is a functional beverage having excellent antioxidant, antidiabetic and anti-obesity activity.

Claims (4)

A process for preparing an Aaronia beverage composition containing active ginsenosides and having enhanced phenolic acid and flavonol,
Iii) steaming goat goat outpost at 100 ° C. for 30 to 60 minutes and repeating high temperature aging 3 to 5 times at 70 to 90 ° C. for 2-3 days to produce active goat ginseng;
Ii) pulverizing the active goat ginseng and extracting the extracted ginseng 2 to 3 times by adding purified water for 4 to 6 hours at 95 to 100 ° C .;
Iii) filtering the active goat ginseng extract, heating to 100 ° C. and concentrating to 15-25 brix;
Iii) mixing 2 to 10 parts by weight of the active goat ginseng concentrate to 100 parts by weight of aronia juice; And
Iii) adding a 2-5% (v / v) subsidiary material to the mixed solution to prepare a beverage,
The submaterial is at least one fruit concentrate selected from the group consisting of strawberries, apples and pears, honey, citric acid and taurine,
The active ginsenoside comprises at least 0.04 mg / g ginsenoside, at least 0.03 mg / g Rd2, at least 0.10 mg / g F2 and at least 0.09 mg / g compound K, wherein the phenolic acid is 3.25 μg of protocatecuic acid. / ml or more, wherein the flavonol is 49.15 μg / ml or more of epicatechin and 149.16 μg / ml or more of quercetin.
delete Prepared by the process according to claim 1, containing active ginsenosides, phenolic acid and flavonol enhanced Aaronia beverage compositions,
The active ginsenoside comprises at least 0.04 mg / g ginsenoside, at least 0.03 mg / g Rd2, at least 0.10 mg / g F2 and at least 0.09 mg / g compound K, wherein the phenolic acid is 3.25 μg of protocatecuic acid. / ml or more, wherein the flavonol is at least 49.15 μg / ml of epicatechin and quercetin 149.16 μg / ml or more of the Aaronia beverage composition.
4. The Aaronia beverage composition of claim 3, wherein the Aaronia beverage composition has enhanced antidiabetic and anti-obesity activity.

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