KR20110037020A - The anti-diabetes composition containing silkworm culturing product and medicinal herbs - Google Patents

Abstract

PURPOSE: An anti-diabetic composition containing sericulture products and herb materials is provided to ensure excellent functionality and physiochemical property for manufacturing beverage. CONSTITUTION: An anti-diabetic composition contains hot water extract of 15 weight% of Morus alba L. fruit, 15 weight% of Mori Folium, 25 weight% of silkworm powder, 15 weight% of Inonotusobliquus, and 15 weight% of Diascorea rhizome. A method for preparing the composition comprises: a step of adding 10 times volume of water to herb materials and sericulture products; a step of extracting the mixture at 75 °C for three hours; and a step of performing sonication for one hour.

Description

The anti-diabetes composition containing silkworm culturing product and medicinal herbs}

The present invention relates to an anti-diabetic composition containing a sheep product and a herbal medicine, and more particularly, to an anti-diabetic composition containing an extract of mulberry and mulberry products of extracts of herbs and chaga mushrooms. .

Diabetes mellitus, the leading cause of death in Korea in 2003, published by the National Statistical Office in 2003, was ranked 4th with 25.1 deaths per 100,000 population. Compared to the 1992 statistics, which ranked 7th in the cause of death, 13.5 people went up three levels, and the mortality rate from complications continues to increase. The cause of this is a decrease in insulin secretion due to the destruction of the pancreatic β-cells due to genetic, metabolic or environmental factors, or hyperglycemia due to insulin resistance in peripheral tissues. It is promoted and causes excessive cell production and cell necrosis such as protein destruction, chromosomal aberration and erythrocyte destruction by excessive production of free radicals (O2-, HO-).

For these free radicals, biological tissues are known to contain endogenous scavengers such as superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px), catalase and glutathione (GSH) and many vitamins A, C, and Bioactive substances such as E, flavonoid pigments and polyphenols protect tissue damage caused by free radicals. However, in case of diabetes, complications such as diabetic retinopathy, stroke, myocardial infarction, chronic renal failure, peripheral neuropathy and hyperlipidemia due to the lack of relative endogenous scavengers due to excessive production of free radicals due to hyperglycemia. Nevertheless, the mechanism is not yet clear, but recently, many experiments have been reported to prevent tissue damage through natural products such as edible and medicinal plants for the prevention or treatment of diabetes and to protect the body.

子)는 뽕나무 열매로서 한방에서 상심(桑

), 상실(桑實), 오심(誤審), 흑심(黑

) 등으로 지칭되며, 뽕나무과(Moraceae)에 속하는 뽕나무(Morus alba L)의 성숙한 과실이다. 오디는 4∼5월에 꽃이 피고 6∼7월에 검은색 또는 자홍색을 나타낼 때 수확한다. 오디는 취화과(聚花果)로 작은 수과(瘦果)가 많이 모여 이루어진 장원형으로 길이 1∼2 cm, 지름 0.5∼0.8 cm이며 황갈색, 갈홍색 또는 암자색을 띠고 짧은 줄기가 있다. 작은 수과(樹果)는 난원형으로 조금 납작한 편이며, 길이는 약 2 mm, 너비는 약 1 mm이고 육질의 화편(花片) 4개가 둘러 싸고 있다. 주치(主治)는 보혈자음(補血滋陰), 생진윤조(生津潤燥), 현훈이명(眩暈耳鳴), 심계실면(心悸失眠), 수발조백(鬚髮早白) 등을 치료하는 효능을 가진다고 알려져 있다.Audi

子 is a mulberry fruit, and it is a heartache

), Loss, nausea, black heart

), And Morus , which belongs to Moraceae. It is a mature error of alba L). Audi is harvested when it blooms in April-May and black or magenta in June-July. Audi is an emollient (과 花果), a small round of aquatic (모여 果) is composed of a lot of 1-2 cm in length, 0.5-0.8 cm in diameter, yellowish brown, brownish red or dark purple with short stems. The small achene is oval, slightly flattened, about 2 mm long, about 1 mm wide, and is surrounded by four fleshy flowers. The main doctor has the effect of treating blood donation consonant, Saengjin Yunjo, Hyun-Hun Lee Myung-Sik, heart-chambered noodles, and hand-to-go baekbaek. Known.

Matured Audi has a good taste with 12.7 ~ 19.8 Brix and 0.29 ~ 0.83% of sugar and acidity, and contains a large amount of anthocyanin pigment. Audi contains large amounts of glucose and fructose, oxalic acid and citric acid, and overall nutrition is higher than general fruit. In particular, the contents of Ca, K and Vitamin C are 14 times, 2 times and 18 times higher than Fuji apple, respectively, and Vitamin C is 1.5 times higher than citrus fruits.

It also has pigments of the antocyanin family and its main components are cyanidin-3-glucoside and cyanidin-3-rutinoside, with a ratio of 7: 3. Anthocyanin pigments have been reported to have various physiological activities such as anti-aging, treatment of diabetic retinopathy, vision improvement, cholesterol suppression, antioxidant and anti-inflammatory effects.

In the Dongbogam tangsui (湯 液 篇), "Black Audi is a mulberry tree spirit (精靈) is gathered, good for diabetes, good for the five chapters, and long eating will make you forget hunger." 'If you eat audio for a long time, it turns black and prevents aging.'

Lee and Chung et al. Observed the hypoglycemic effect of silkworm-related substances, and reported that they control the rapidly rising blood sugar level by competitively inhibiting α-glucosidase, an enzyme that degrades intestinal carbohydrates.

In 1985, Kikino et al. Isolated the glycoprotein Moran A from the epidermis and announced that it was a hypoglycemic activity. Kimura et al. Reported that streptozotocin-induced diabetic mice have potent hypoglycemic activity in the upper lobe. Said. According to these studies, the hypoglycemic action of the upper lobe was reported as a mechanism different from that of insulin secretion or insulin-like action. Chung et al. Myo- is known to have a therapeutic effect on diabetic complications by normalizing the nerve conduction rate to the upper lobe. It was confirmed that a large amount of inositol.

In studies conducted by fractionating and extracting active substances from lettuce and silkworm powders and testing them in experimental animals, these active substances inhibited the action of α-glucosidase in the intestine and delayed the digestion and absorption of sugars. Has a concentration-dependent effect.

According to kim, water fractions of the medicinal perennial plant, Sans, Diascorea rhizoma, have a hypoglycemic effect by promoting insulin secretion in a type 1 diabetes animal model, and Hikino et al. In the experimental model, the hypoglycemic effect component of powder was reported as Dioscorans. Powders have also been reported to affect immune function in the body and improve related diseases. Reduced immune disease, anti-inflammatory effects, suppression of tumor growth and improvement of osteoporosis have been reported.

Morio Folium is a representative herb used in herbal medicine for thirst and is known to be involved in the digestion of carbohydrates in the small intestine and its hypoglycemic activity has been reported to be associated with postprandial glycemic control.

Chaga is a black birch carcinoma that grows in Russia and its scientific name is Inonotusobliquus. The mushroom belongs to the family Hymenochaefaceae and forms a large fungal nucleus in the form of tubers on wound birch. Ichimura et al. Reported that chaga water extract inhibited the protease of humanimmunodrficiency virus type 1 and that the substance was a water-soluble lignin of the polymer. On the other hand, studies have shown that the water-soluble and insoluble glycoproteins isolated from chaga have the effect of lowering blood sugar by regulating the cell cycles of the glycemic control cdc25 and cdc2 / cyclin B.

Therefore, in the present invention, synergistic complexes for type 2 diabetes diseases were selected for the combination of optimal results of the selection of sheep products and herbal medicines whose glycemic activity was reported in the literature or whose antidiabetic activity was verified in the study of the present inventors. To investigate the efficacy, we tried to demonstrate antidiabetic activity in streptozotocin-induced rats. In addition, to find the optimal ratio in using the composition as a beverage.

The present invention has been made under the above-mentioned background, and an object of the present invention is to provide a composition suitable for beverage in terms of anti-diabetic function, physicochemical properties, and sensory evaluation by mixing the ingredients of a sheep product and herbal medicine in an optimal ratio. .

The present invention is to achieve the above object, the anti-diabetic composition using the bile products and the herbal medicine of the present invention is 15% by weight of Audi, 15% by weight of the upper limbs, 15% by weight of the leaves, silkworm powder 25% by weight, chaga mushroom 15% It is characterized by containing the hot water extract of the sum of 15% by weight, acid.

At this time, the hot water extract was added 15 times by weight of Audi, 15% by weight of upper limbs, 15% by weight of upper leaves, 15% by weight of silkworm powder, 25% by weight of silkworms, 15% by weight of chaga mushrooms, and 15% by weight of medicinal herbs. After extraction for 3 hours at ℃ it is characterized in that obtained by performing the ultrasonic extraction for 1 hour to promote the extraction of the active ingredient.

According to the present invention having the above-described configuration, by optimally mixing the components of the sheep probiotics and the herbal medicine can be obtained a composition suitable for beverage in terms of anti-diabetic function, physicochemical properties, and sensory evaluation.

Hereinafter, the present invention will be described in detail with its experimental examples and examples.

1. Materials and reagents

Yangjam products were purchased from Yeongcheon Yangjam Union and herbal medicines were purchased from Daegu Yangnyeongsi. The reagents used in the experiment were Indophenol, 2,4-Dinitrophenyl hydrazine, L-ascorbic acid, Tannic acid, and ACS reagent. (St. Louis, Mo., U.S.A.) and used. 1-1-diphenyl-2-picrylhydrazyl (DPPH), Pyrogallol, Trichloroacetic acid (TCA), Griess reagent (sulfanilic acid, naphtylamine), etc., which were used in the antioxidant validation experiment, were identified as Sigma Chemical Co. (St. Louis, MO., U.S.A), 2-thiobarbituric acid (TBA) was purchased from Eastern Organic Chemical (Roochester, NY.) And used. Other reagents were used as express reagents.

The instrument used in the experiment was UV / vis spectrophotometer (Hitachi, Japan), Rotary vacuum evaporator (Tokyo, Rikakikai Co., Japan), Centrifuge (Hitachi, Japan), pH meter (Model DP-135M), Color meter (Minolta, CR-300, Japan), Autoclave (SH-AC60, Korea), ICP Atomic Emission Spectrometer (ICP-IRIS, Thermo Elemental, USA), and the like.

2. Extraction of Yangmyeon Products and Medicinal Herbs

In the experiment of the present invention, silkworm powder, upper limbs, upper leaves, audi and herbal medicines (chaga mushrooms, herbal medicines) were used as a sheep submerged products. Yangmyeon product and Chinese herbal medicine were extracted with hot water (10 times water to Yangmyeon product twice, extracted twice at 75 ℃ for 3 hours), and then ultrasonically extracted for 1 hour to promote the extraction of active ingredients and concentrated to 20 Brix. Then it was used in this experiment.

Sampling conditions and compounding ratios for the functional evaluation of Yangshum products and herbal medicines are as follows. Sample concentrations were 0.1%, 1% and 2%.

3. Functional evaluation of Yangshum products and herbal medicines

1) Total polyphenol content

Total polyphenol content was determined by the Folins-Denis method, which is widely used as an analytical method. 1 ml of Folin ciocalteu's phenol reagent was added to 3 ml of the sample solution, and 1 ml of saturated Na ₂ CO ₃ solution was added thereto, followed by reaction at room temperature for 1 hour, and 0.2 ml of 1 N HCl was added to measure absorbance at 640 nm. At this time, the total polyphenol compound content was calculated from the standard curve using tannic acid.

The standard curve is to dissolve tannic acid in distilled water at a concentration of 10 mg / ml and to make a final concentration of 0, 37.5, 75, 150, 300 ㎍ / ml solution. UV / VIS Spectrophotometer (hitachi U-2001, Japan) Was prepared by measuring the absorbance at 640 nm.

2) Measurement of electron donating ability

The electron donating ability was measured by modifying Blois' method. 1.0 ml of 2 × 10 ⁻⁴ M DPPH was added to 2 ml of each sample, vortexed, and allowed to stand for 30 minutes, followed by measurement of absorbance at 517 nm. The electron donating ability was expressed as [1- (absorbance of sample addition-absorbance of sample / absorbance of sample no addition)] × 100.

3) Superoxide dismutase (SOD) -like activity measurement

The SOD-like activity was measured by adding 3 ml of tris-HCl buffer (50 mM tris [hydroxymethyl] aminomethane + 10 mM EDTA) and 0.2 ml of 7.2 mM pyrogallol to 0.2 ml of each sample according to Marklund and Marklund's method. After leaving for 10 minutes at 1 ° C., the reaction was stopped with 1 ml of 1 N HCl, and the absorbance was measured at 420 nm. The absorbance at 420 nm was measured as [1- (absorbance of sample / absorbance of sample)] × 100.

4) Determination of Xanthine oxidase Inhibition

Xanthine oxidase inhibitory activity was measured according to the method of Stripe and Corte. That is, 0.2 ml of 2 ml xanthine and 0.1 ml of xanthine oxidase (0.2 unit / ml) were added to 0.6 ml of 0.1 M potassium phosphate buffer (pH 7.5) and reacted at 37 ° C. for 15 minutes. 1 ml of 1 N HCl was added thereto to terminate the reaction, and the absorbance of the uric acid generated in the reaction solution was measured at 292 nm.

5) α-Glucosidase Inhibitory Activity

α-Glucosidase inhibitory activity was measured by slightly modifying the method of Haglind and Tengblad. Dissolve ρ-nitro-phenol-α-D-glucopyranosite in 0.1 M phosphate buffer (pH 7.0) as a substrate, mix 5 units / 0.03 ml of enzyme solution with 0.1 ml of distilled water in the control, and 0.1 ml of sample in the reaction. After the reaction for 30 minutes at 37 ℃ 1 M glyciner NaOH (pH 9.0) was added to stop the reaction. The ρ-nitrophenol (PNP) produced at this time was measured for absorbance at 405 nm by spectrophotometer, and the inhibitory activity was obtained by the following equation.

6) Statistical Processing

All experiments were repeated three times and expressed as mean ± standard error. Each test result was analyzed by p-0.05 by one-way ANOVA (Analysis of variance) using SPSS 12.0 statistical program, and significant difference between groups was analyzed according to Duncan's multiple range test. It was.

4. Results of Functional Evaluation of Yangjam Products and Medicinal Herbs

1) Total Polyphenol Content

The total polyphenol content in the combination ratio of Yangjam products and herbal extracts 1 was 29.25mg / g in 0.1%, 57.96mg / g in 1% and 77.81mg / g in 2%. At 0.1% of the combination ratio of the two-produced products and herbal extracts, it was 55.42mg / g at 1% and 77.28mg / g at 2%. At 0.1% of the combination ratio of Yangjam products and herbal medicine extracts, it was 44.55mg / g at 1% and 57.69mg / g at 2%. At 0.1% of the combination ratio of Yangjam products and herbal extracts 4, it was 24.96mg / g, 50.05mg / g at 1%, and 65.49mg / g at 2%. As the silkworm content increased, the total polyphenol content tended to decrease, and as the upper limbs, upper leaf, mulberry (audio) and herbal medicines increased, the total polyphenol content tended to decrease.

Phenolic compounds are generally known to represent antioxidants. Cha et al. Reported that the extraction of phenolic substances from Audi with different extraction solvents showed higher elution of phenol in the experimental group extracted with ethanol or acetone than with distilled water. In addition, the extraction of phenolic compounds with various concentrations of ethanol as the extraction solvent was reported to be the highest at 60% ethanol concentration.

Many studies have reported that the main role of phenolic compounds is to eliminate free radicals. Therefore, the total phenolic content, such as the total amount of flavonoids, phenolic acids, and anthocyanins, which are phenolic compounds, acts as an important factor in the antioxidant activity represented by DPPH radical scavenging ability. In general, as the antioxidant activity increases, the total phenolic content also increases. In addition, vitamin C (ascorbic acid) is a representative water-soluble vitamin and is often used as an indicator of nutrition since it represents representativeness compared to other nutrients contained in food.

The total phenolic contents of the bokbunja varieties ranged from 20.89 to 28.84 mg / g. These results showed that the total polyphenol content was relatively higher than the total polyphenol content of Bokbunja (19.87∼77.81 mg / 100g).

2) electron donating ability

As a result of the measurement of the electron donating ability of the extracts of Yangjam products and medicinal herbs, the higher the concentrations of BHA, the higher the concentrations, were 79.0%, 87.2%, and 95.67%. The first and second extracts from Yangyang products and herbal medicines showed 11.7% at 1%, 19.45% at 1%, and 23.75% at 2%. In the two samples, Yangjap products and medicinal herb extracts showed 38.9% at 2%, 48.88% at 2%, and 58.35% at 3%. The three probiotic and herbal extracts 3, 48.36% at 2%, 59.21% at 2%, and 66.61% at 2%. The sample of Yangjam products and herbal extracts 4, 51.63% at 0.1%, 67.13% at 1%, and 71.43% at 2%.

Overall, high electron donating ability was observed in the sample sphere containing much silkworm powder. Kim et al. Used DPPH radical scavenging method and BHA as a reference drug to additionally search for antioxidant activity against nine species. As a result, almost all of the nine detected species showed antioxidant activity. Among them, Shingwang pong (70.4%) showed the highest antioxidant activity, followed by gumsul pong (52.0%)> double doucus (38.4%)> quadruple hibiscus (35.7%)> cheong il pong ( 32.9%).

Silkworm powder was tested for the production of oxidized protein by measuring the amount of carbonyl group produced by the attack of active oxygen in the experiment of inhibiting the production of oxidized protein. As a result, 35.42 ± 4.54 and 34.81 ± 3.78 nmol / mg protein were used. 89.2% and 87.6% of the amount of production, respectively, significantly inhibited the production of oxidized protein by silkworm powder administration. This has proven to be very effective in inhibiting the oxidation of lipid and protein components. Therefore, the silkworm powder can effectively inhibit the oxidative stress in spite of the difference according to the dosage. Therefore, the silkworm powder is expected to be able to effectively suppress the aging due to the very good antioxidant action. In this experiment, as the blending ratio of silkworm powder is increased, the DPPH content is increased, which contributes to suppressing oxidative stress.

On the other hand, Park et al measured antioxidant activity by measuring free radical scavenging ability according to the concentrations of five varieties of bokbunja strawberry and blackberry. Antioxidant activity of the five kinds of bokbunja strawberry was higher than that of blackberry, and the antioxidative activity of Kumjung No. 1 was 78.55% and 50.48% at 2 mg / ml and 1 mg / ml, respectively.

3) Superoxide dismutase (SOD) -like activity measurement

As a result of measuring SOD-like activity of the extracts of Yangshuk and Chinese herbal medicine, vit. C was 100%, and the first and second herbal and herbal extracts were 17.74%, and the four and four extracts were 72.9%. The Yangjam product and the herb extract 4 showed higher tendency than the Yangja product and the herb extract No. 1, and the SOD-like activity of the Yangjam product and the herbal extract used in this experiment was higher than 41.79% of Astragalus Park and 44.3% of the pine needles of Kim. The performance tended to be higher.

Antioxidants superoxide dismutase (SOD), one of the enzyme reaction for telephone harmful reduced oxygen species in the cells with ^hydrogen peroxide and an enzyme catalyzing the ^{_{(2O 2 + 2H + → H}} 2 O 2 + O 2), produced by the SOD H ₂ O ₂ is known to protect the body from oxygen injury by converting it into harmless water and oxygen molecules by peroxidase or catalase. SOD-like substances are not enzymes but are low-molecular substances that play a similar role to SOD. They are primarily phytochemicals and can inhibit oxidative disorders by inhibiting the reactivity of superoxide. Nice et al. Reported that phenol-type materials combined with SOD were purified by refining the SOD-purifying material with excellent thermal stability and showing similar activity with SOD.

4) Determination of Xanthine oxidase Inhibition

Inhibition rate of xanthine oxidase from the extract of Yangshuo and Chinese herbal medicines was measured. C represented 73.82%. At the sample concentration of 2%, the amount of Yangmyeon product and medicinal herb extract was 11.79%, and that of Yangmyeon product and medicinal herb extract 4 was 52.04%. Indicated.

Xanthine oxidase, a free radical production system in vivo, is an enzyme involved in purine metabolism. During this process, urate is formed from xanthine or hypoxanthine (free radical generation). It is known to be an enzyme that causes kidney disease caused by gout and kidney deposition. Many studies have been conducted on natural products for the purpose of inhibiting enzyme activity. The flavonoids present in plant systems have different xanthine oxidase inhibitory effects depending on the position of hydroxyl groups, and flavonoid compounds containing galloyl groups inhibit xanthine oxidase. Reported excellent. Therefore, the inhibitory effect of xanthine oxidase is of biological significance as well as inhibition of free radical production.

  Moon et al. Reported that xanthine oxidase inhibitory effect increased as the concentration of extract increased.

  Xanthine oxidase inhibitory agents are representative of flavonoides, and xanthine oxidase inhibitors isolated from onion peels were identified as quercetin 4'-glucoside and quercetin. In addition, An et al. Reported that flax-3-ol compound was identified as a result of separating xanthine oxidase inhibitory activity from oolong tea and exhibited 60.6% inhibitory activity at 50 μmol addition.

5) Inhibitory Effect of α-glucosidase Extracts

The inhibitory activity of α-glucosidase on the extracts of Yangjam products and medicinal herbs was 11.76% ~ 82.84%. The first probiotic and herbal extract No. 1 showed 11.76% in sample concentration, 11.76% in 1%, 25.62% in 1%, and 43.01% in 2%. The two samples and the herbal extracts, 63.05%, 0.1%, 68.58% and 2%, 70.54%, respectively. The three probiotic and herbal extracts 3, 75.67% at 1%, 75.73% at 1%, and 78.37% at 2%. The sample of Yangjam and Herbal Extract 4 was 77.83% at 0.1%, 79.17% at 1%, and 82.84% at 2%. The anti-diabetic test substance, a-glucosidase inhibitory effect, showed that antidiabetic activity was improved as the amount of silkworm powder increased.

As a result of the α-glucosidase inhibitory activity of trigeminal leaf vinegar extracts, domestic and Chinese trigeminal leaf vinegar showed 87% and 88% higher inhibition rate than water and methanol extracts. To date, the hypoglycemic effect of the upper lobe or silkworm was found to inhibit the disaccharide degradation of α-glucosidase in the small intestine.

5. Preparation and Evaluation of Sheep Produce and Herbal Medicine Complex

As a result of evaluating the functionalities according to the blending ratio of sheep probiotics and herbal medicines, the higher the ratio of silkworm powder, the more the antioxidant activity tended to increase. However, as a result of sensory evaluation (for example, Table 5 below) and physicochemical evaluation, the amount of silkworm powder added was 25%, and the leaves, upper limbs, heart beats and herbs, chaga mushrooms were prepared. Mixing the ratio to 15% was rated as the most suitable for prototype quality. Therefore, the compounding ratios of Yangmyeon product and herbal medicine complex are shown in Table 3.

6. Sugar, pH, Acidity and Color Difference of Antidiabetic Prototype

Table 3 shows the main ingredient blend ratios for measuring the antidiabetic effect of biproduced products. Sample concentrations were 0.1%, 1% and 2%. For the extract of Yangjam products, Youngcheon mulberry, upper limbs, upper leaves and chaga, and acid powder were used.

After preparing anti-diabetic composition by using Yangjam products and herbal extracts, the results of measuring sugar, pH, acidity and color difference are shown in Table 4. The sugar content was 15 Brix, pH 3.3, acidity 0.3, lightness 37.33, redness 37.41, and yellowness 24.58.

7. Sensory Evaluation of Antidiabetic Compositions

For the sensory evaluation of antidiabetic composition, students from Daegu Haany University were selected to explain the experimental purpose and evaluation items, and sufficient training was conducted to construct a specialized panel with the ability to identify the quality difference of antidiabetic composition. And for a broader marketability test, sensory evaluation was conducted for all students of Daegu Haany University. Sensory evaluation was performed by five-point palatability test, and sensory evaluation items were color, flavor, taste, and overall acceptability. As a result of sensory evaluation, sheep subproduct 1 showed a high sensory test result with a comprehensive acceptance degree of 4.1 ± 1.68. As silkworm powder content increased, the sensory evaluation showed lower evaluation in color, aroma, taste and overall preference. However, in the two-product product 2, silkworm powder added was 25% and the other compounding ratio was 15%, and the sensory test showed 4.0 ± 1.72. Therefore, it is expected that the selection of bilateral product 2, which contains 25% of silkworm powder, with excellent antioxidant and α-Glucosidase inhibitory ability, will be well received in terms of consumer preference and efficacy. Used for.

In the embodiment of the present invention, the food can be prepared by adding other ingredients based on the above results and referring to the food specification suggested by the Food and Drug Administration in addition to the combination ratio of the two-armed product and the herbal medicine.

Specifically, for the manufacture of food for diabetics, vitamin A, B1, B2, B6, C, D, E, niacin, folic acid, protein, calcium, iron, and zinc are combined so that 50% or more of the nutrient standard is used. Nutrients can be added.

8. Evaluation of Animal Experiments of Combination of Sheep Produce Products and Herbal Resources

Serum lipid test and blood glucose test of diabetic rats using effective extracts of chaga, medicinal herb and sheep proboscis showed that anti-diabetic groups such as chaga were more effective than diabetic rats. . This experiment, on the other hand, is based on the formulation of bimodal product 2.

(1) Laboratory Animals and Diet

end. Experimental group: Animals used in this experiment were Sprague Dawley male rats with an average body weight of 180 ± 10g and were divided into 4 groups of 10 animals per group by the egg mass method for 10 days of adaptation to general solid feed. In other words, the experimental group was divided into the normal control group, the diabetic control group, the diabetic induction product and the herbal ingredient extract supply group.

I. Diabetes Induction: Diabetes induced streptozotocin was dissolved in fresh 0.01M citrate buffer at pH 4.5 and injected once intraperitoneally at 55mg / kg BW. Normal group injected 0.01M citrate buffer intraperitoneally to give the same stress as the diabetic group. It was. Diabetes was confirmed 3 days after streptozotocin injection from the tail vein and the diabetes was considered to be induced when the fasting blood glucose level is 300mg / mL or more.

All. Breeding conditions: The diet and water of the experimental animals were freely ingested during the experimental period in which the samples were fed after the induction of diabetes. Breeding conditions were individually bred in stainless steel cage, temperature 22 ± 2 ℃, humidity 55 ± 5%, contrast cycle 12 hours (day light 06: 00-18: 00).

la. Sample feeding method: The two-produced products and chaga extract were concentrated under reduced pressure using a rotary vaccum evaporator and lyophilized, and orally administered once daily at 350 mg / kg B.W. Sheep products were extracted with 10 times the weight of water, filtered, concentrated under reduced pressure, lyophilized, and administered orally once a day at 150 mg / kg B.W.

(2) Experiment Method

end. Blood sampling: Blood collection from experimental animals was performed by anesthetizing the rats with ether, and opening the rats. Using a 21-gage disposable syringe, blood was collected from the abdominal aorta, and the blood was left at room temperature for 2 hours, followed by centrifugation at 600xg for 15 minutes to separate serum. It was.

I. Blood glucose measurement: After fasting for 12 hours before blood glucose measurement, blood was collected from the tail vein and measured by liver glucose meter.

All. Lipid analysis: Total lipid content in serum was measured using a kit for measuring total lipid content (Advanced diagnostic Inc., USA). Neutral lipid content was an enzyme method according to Muller's method. Total cholesterol content and HDL-cholesterol content were The serum lipid content measurement kit (Eiken Co.) prepared according to Richmond's method was measured. Lipid content of liver tissue was 1.0g of liver tissue, and 10% tissue grinding solution was prepared with 0.15M NaCl solution using a homogenizer, and then chloroform: methanol (C: M = 2: 1 v / v) mixture was prepared according to the method of Folch et al. Lipids were extracted and measured in the same way as serum.

9. Anti-diabetic Experimental Evaluation of Combination of Sheep Produces and Herbal Medicines

(1) weight change

Table 7 shows the results of measurement of weight change after breeding for 2 weeks with each sample fed to streptozotocin-induced diabetic rats. The diabetic group showed a significant decrease compared to the normal control group, and the weight gain was increased in comparison with the diabetic control group when the extracts of the sheep probiotics or the combination of the sheep probiotics and the herbal medicines were increased, but there was no significant difference.

(2) long-term weight

Table 8 shows the results of long-term weight change after two weeks of feeding each sample to diabetic rats and non-induced rats. The liver weight per 100g of body weight was significantly increased in the diabetic induction group compared to the normal control group, and there was no significant difference according to the supply of sheep product and herbal medicine combination. Kidney weight tended to be equal to liver weight. There was no significant change in the weight of the spleen and heart.

(3) Determination of Serum ALT and AST Activity

Alanine aminotransferase (ALT) is an enzyme that produces tissue energy. Unlike AST, it is abundant in the cytoplasm and distributed in the kidneys, heart, and skeletal muscle. It is an enzyme that is distributed in skeletal muscle and leaks into serum when cells are damaged. Table 9 shows the results of serum ALT and AST measurements. Serum ALT activity was significantly increased in the diabetic control group compared to the normal control group, and significantly decreased in the sheep probiotics and herbal medicine combination groups compared to the diabetic control group. Serum AST activity was significantly increased in the diabetic control group compared to the normal control group, and significantly decreased in the diabetic and herbal supplements.

(4) change in blood sugar

Fig. Figure 7 shows the results of blood glucose measurements from blood collected from the tail vein of rats induced by diabetes by intraperitoneal injection of Streptozotocin. Diabetes is considered to be induced when the blood glucose level is higher than 300mg / ml due to blood collection. During the experimental period, the normal control group showed little change in blood sugar level and the diabetic control group increased blood sugar rapidly until 1 week of diabetes, but 1 week after 2 weeks. It remained at about the same level as. Blood glucose levels in both groups were significantly reduced from 1 week to 2 weeks, and were significantly lower than those of the diabetic control group.

(5) lipid content in serum

Fig. 8-11 is a result of measuring the lipid content in liver tissue. The total cholesterol content in serum was significantly increased in the diabetic control group compared to the normal control group, and significantly decreased compared to the diabetic control group in the combination of bilateral product and herbal medicine. Serum triglyceride content was significantly increased in the diabetic induction group compared to the normal control group and decreased in comparison with the diabetic control group in the combination of both sheep product and herbal medicine, but there was no significant difference. Serum total lipid content and HDL-cholesterol content did not change significantly.

Claims (2)

15% by weight of Audi, 15% by weight of upper limbs, 15% by weight of upper leaves, 25% by weight of silkworm powder, 15% by weight of chaga, 15% by weight of hot water extract, Anti-diabetic composition using sheep probiotics and herbal medicines. The method of claim 1, The hot water extract consists of 15% by weight of Audi, 15% by weight of the upper limbs, 15% by weight of the upper leaves, 25% by weight of silkworm powder, 15% by weight of chaga mushrooms, 15% by weight of medicinal herbs and herbal medicines. After extracting for 3 hours and performing ultrasonic extraction for 1 hour, characterized in that obtained by promoting the extraction of the active ingredient, Anti-diabetic composition using sheep probiotics and herbal medicines.