KR100856083B1 - Method for efficient preparation of ginseng extract containing high level of red ginseng-specific saponin - Google Patents

Abstract

A method for efficiently preparing ginseng extract having a high content of red ginseng-specific saponin by treating ginseng in a high temperature and high pressure liquid solvent is provided to control the concentration of the red ginseng-specific saponin quite freely and to manufacture the red ginseng-specific saponin. Ginseng is treated with a high temperature and high pressure liquid solvent for 30 to 50min to extract saponin. The liquid solvent is obtained by heating ethanol at 190 to 240deg.C and 1 to 10MPa. The ginseng extract contains more than 90% of ginsenoside Rg3, based on ginseng total saponin. The saponin extract is selected from the group consisting of ginsenoside Rg3, ginsenoside Rg2 and protopanaxatriol.

Description

Method for efficient preparation of ginseng extract containing high level of red ginseng-specific saponin}

1 is a conceptual diagram showing an extraction apparatus for extracting saponins from white ginseng using a pressurized heat medium according to an embodiment of the present invention.

Figure 2 is a comparison result of the change in the content of the total saponin 11 species when the extraction temperature, time and different types of solvents for white ginseng.

Figure 3 is a result of comparing the change in the content of ginsenoside Rg ₃ when the extraction temperature, time and solvent type of the solvent for the white ginseng were different.

Figure 4 is a comparison result of the change in the content of ginsenoside Rh ₂ when the extraction temperature, time and the type of solvent of the white ginseng was different.

5 is a result of comparing the change in the total content of ginsenoside Rg ₃ and ginsenoside Rh ₂ when the extraction temperature, time and solvent type of the solvent is different with respect to white ginseng.

6 is a result of comparing the change in the total content of ginsenoside Rg ₃ and ginsenoside Rh ₂ compared to the total saponin content when the extraction temperature, time and type of solvent of the white ginseng was different.

The present invention relates to a method for extracting and converting saponin of red ginseng into red ginseng-specific saponins, and more specifically, preparing a high-temperature, high-pressure liquid solvent by pressing and heating water or an alcohol having 1 to 6 carbon atoms, and A method comprising extracting saponin by treating a high pressure liquid solvent with hemp and extracting saponin from edible acid or mixed with alcohol and acid to extract saponin into red ginseng-specific saponin. It is about.

Panax ginseng CA Meyer is a perennial herb belonging to the genus Araliceae Panax ginseng, tonic, gangjeong, hematopoietic, thermal insulation, fatigue recovery, mental stability It is known to have sedative effects, and its root is called Ginseng radix and is used for food and medicinal purposes.

The efficacy of these ginseng is mainly due to ginseng saponin (ginsenoside) has been studied a lot. Ginseng saponins are composed of sugar bonds such as glucose, rhamnose, xylose, arabinose and esters of alcoholic OH groups of R1, R2 and R3 of triterpene, protopanaxadiol and protopananatriol. More than 30 kinds of ginseng saponins have been identified.

These ginseng saponins are not toxic, unlike other plants, and are not known to exhibit hemolytic action below 0.001%. Elimination effect, anti-fatigue and anti-stress action, antioxidant action, anti-inflammatory activity, protein synthesis promoting action, immune enhancing action and the like are known.

Among saponins, saponins are present only in red ginseng, which is produced by steaming and drying ginseng, and specific ingredients such as ginsenosides Rg ₂ , Rg ₃ , Rh ₁ , and Rh ₂ present in small amounts in red ginseng prevent cancer. It has been found to be effective in inhibiting cancer cell growth, lowering blood pressure, protecting nerve cells, antithrombotic action, antioxidant activity, and the like.

The general manufacturing process of red ginseng is to wash the fresh ginseng with water, steam it in a certain container using heated steam, steam it for a certain time according to its size, and heat-dry it first, and then use solar heat or other The method consists of drying until the moisture reaches 12.5 to 13.5%, and through the process of drying and removing the malonyl group of malonyl ginsenosides, leaving the glycosyl group and isomerizing the hydroxyl group. The reaction of the back causes a variety of saponin components specific to red ginseng. However, the manufacturing process of the red ginseng is an inefficient process that consumes a lot of time and money, and has become a factor that inhibits the popularization of red ginseng.

On the other hand, a method of extracting useful substances from a plant is generally a method of heating in a vessel at atmospheric pressure (ie, 100 ° C. or lower), or batch extraction in a pressure resistant container such as an autoclave. The method is mainly used (for example, Japanese Patent No. 3,212,278). However, these conventional methods have limitations in terms of technical aspects such as the difference in extraction ability occurs depending on the plant to be extracted and the components to be extracted.

In order to overcome the limitations of the existing extraction method, an extraction method for extracting an extraction solvent using a high temperature and high pressure liquid solvent has been developed (Korean Patent Publication No. 2005-103804).

Therefore, the present inventors studied by applying the above extraction method in order to develop a method that can easily obtain useful saponin components, the saponin existing in the ginseng as red ginseng-specific saponin when the extract solvent is heated and pressed on the ginseng Is converted to a large amount, thus red ginseng specific ginsenoside Rg ₃ or Rh ₂ It was found out that a saponin extract containing a large amount of the back can be prepared, and thus, a saponin extraction method was developed to complete the present invention.

Accordingly, an object of the present invention is to provide a method for extracting the conversion of saponin from ginseng which can be extracted by converting saponin of ginseng into red ginseng-specific saponins.

It is another object of the present invention to provide a saponin extract from which saponins are extracted by a conversion extraction method.

It is also an object of the present invention to provide an economical and efficient saponin extract in a batch form, and to provide a method capable of freely adjusting the concentration of red ginseng-specific saponins.

In order to achieve the above object, the present invention

(a) pressurizing an alcohol having 1 to 6 carbon atoms to 1 to 10 MPa and heating to 100 to 240 ° C. to prepare a high temperature, high pressure liquid solvent; And

(b) preparing a method for extracting the conversion of ginseng to red ginseng-specific saponins, comprising the step of extracting saponins by treating the high-temperature, high-pressure liquid solvent in step (a) with ginseng; Provided is a method of extracting red ginseng-specific saponins and compounds comprising K, PPD, and the like.

Hereinafter, the content of the present invention will be described in more detail.

The conversion extraction method of saponin of the present invention is characterized in that it comprises the steps of preparing a liquid solvent of high temperature, high pressure by pressing and heating the extraction solvent and extracting saponin by treating the liquid solvent of high temperature, high pressure in three .

Referring to the method of extracting the conversion of the saponin step by step.

(A) step: pressurizing and heating the extraction solvent to prepare a liquid solvent of high temperature, high pressure

In the present invention, the extraction solvent may be an edible single or plural kinds of solvents, but preferably an alcohol having 1 to 6 carbon atoms. The alcohol may preferably be ethanol, methanol, butanol, pentanol or alcohol, more preferably ethanol or alcohol. The ethanol may preferably have a concentration of 1% to 99%, more preferably may have a concentration of 70% or 99%. The solvent is the inventors selected the most suitable type for the extraction of a particular saponin through the experiment.

That is, the present inventors compared the extraction efficiency after extracting saponins from white ginseng using a solvent of various types and concentrations, it was confirmed that it is most preferable to use water and ethanol to extract the total saponins. However, the conversion extraction to red ginseng-specific saponin could be preferably performed when using ethanol, ethanol was found to have the highest extraction efficiency when 70% to 99%.

On the other hand, in the present invention, the pressure of the solvent is to maintain the solvent in the liquid phase at a predetermined temperature, thereby maximizing the conversion of the saponin of hemp into red ginseng specific saponin. At this time, the pressurization may be performed according to a conventional method well known in the art such as a high pressure pump, it is preferable to perform at 1 to 10 MPa.

Heating of the solvent may be carried out using conventional heating means such as, for example, an electric heater, and heating may be carried out simultaneously or sequentially with pressurization. At this time, heating temperature is 100-240 degreeC, Preferably it is 140-220 degreeC, More preferably, it has the range of 160-200 degreeC. If the temperature is less than 100 ℃ can not be efficiently extracted by converting saponin, if the temperature exceeds 240 ℃ energy is consumed in a large amount is not economical.

In order to overcome the limitations of the conventional extraction method, such pressurization and heating are intended to prepare the extraction solvent as a high-temperature, high-pressure liquid solvent. More specifically, the solvents such as water and ethanol have high temperature and high pressure just below the supercritical point. Under the condition of, it exists in a liquid state. In this case, the ionic value of water at room temperature and atmospheric pressure is 1 X 10 ^-14 , for example, in the case of liquid water at 250 ° C, the ionic group is about 1 X 10 ^-11 It increases by about 1,000 times and has functions such as acid and alkali. However, because the supercritical extraction zone is ultra high temperature, it is impossible to extract because the compound of functional ingredient is completely burned. Supercritical extraction using carbon dioxide is not used in super high temperature zone, but it is currently used, but the scope of application such as caffeine removal is limited. There is a limit to applying a threshold. However, it is possible to expect a high extraction capacity by using a temperature of a somewhat milder condition and a pressure condition that can maintain the solvent of this temperature in the liquid phase.

(b) step: extracting saponin by treating the liquid solvent at high temperature and high pressure with hemp

The high temperature, high pressure liquid solvent heated under pressure in step (a) is treated with hemp and converted to specific saponin to extract. In this case, the term 'treatment' in the present invention means that the extraction solvent is converted by adding the extraction solvent to hemp as a raw material, and the extraction may be performed, and the extraction may be performed according to a conventional extraction method known in the art. .

In the present invention, hemp may be commercially available hemp, preferably ginseng ( Panax ginseng CA Meyer, Panax ginseng quinquefolium ) Panax Notoginseng ( Panax notoginseng ), Panax ginseng ( Panax japonicum ), Trilobite ( Panax trifolium ) or Himalayan ginseng ( Panax pseudoginseng ), and any site can be used without covering the site. More preferably, it may be ginseng, and may be ginseng or white ginseng, depending on the processing form. At this time, when extracting saponin, hemp may be used as a whole, but may be preferably chopped or milled to increase the extraction efficiency.

The extraction time of the saponin is preferably 10 to 240 minutes. If the extraction time is less than 10 minutes saponin extraction efficiency is lowered, if it exceeds 240 minutes it is not preferable because it is uneconomical.

The saponins to be extracted are ginseng saponins (ginsenoside) known in the art, preferably red ginseng specific saponins, more preferably ginsenoside Rg ₃ , ginsenoside Rh ₂ and PPT (protopanaxatriol; Park et al., Planta Med) 72 (13): 1250-1253, 2006). Such red ginseng-specific saponins are specifically present in red ginseng, and are produced by converting ginseng saponin by the conversion extraction method of the present invention.

Finally, the saponin extracted from the conversion is recovered. The recovery method may be preferably performed according to a general recovery method known in the art, such as drying or freeze drying.

In addition, the saponin extraction method of the present invention may be by an acid treatment process edible alone or by extraction with a mixture with alcohol. In the acid treatment process, the extraction solution is converted into an acid solution in step (a) for 10 to 90 minutes, and the temperature is preferably treated within 120 ° C.

Acids used in edible acid treatment include vinegar (brewing vinegar, pH 7%, pH 2.47), persimmon vinegar (p. 3%, pH 3.45), citric acid (pH 5.02), acetic acid (glacial acetic). acid, pH 0.27), double brewing vinegar (about 14% acidity, pH 2.30). In more detail, it is preferable to use an acid pH of 5.5 or less.

The saponin extracted after the acid treatment may be red ginseng specific saponin, preferably compound K (20-O-β-D-glucopyranosyl-20 (S) -protopanaxadiol) or PPD (protopanaxadiol). Such red ginseng-specific saponins are not present in ginseng other than red ginseng, and are produced by converting ginseng saponin by the conversion extraction method of the present invention.

The specific extraction method and the configuration of the saponin conversion extraction apparatus used in the present invention according to an embodiment of the present invention is as follows (see Fig. 1), but is not limited thereto.

Extraction apparatus according to an embodiment of the present invention is to improve the percolated extraction method of Republic of Korea Publication No. 10-2005-0103804 by using a batch method (batch). The percalate method extracts the active ingredient by releasing the solvent under high pressure using a high pressure pump, while the batch method is a method of extracting the active ingredient by simultaneously supplying a constant solvent, temperature and pressure. Therefore, it can be said that the rapid response to the high economical time, and the high availability for mass production and industrialization.

In addition to the batch extraction method of extracting the raw material by treating a predetermined amount of the extraction solvent, the present invention can be carried out continuously (a) to (b) to extract the saponin by a continuous extraction method. In this case, the continuous extraction method refers to a method of continuously supplying the extraction solvent into the extraction apparatus at a constant flow rate and simultaneously extracting the extract out of the extraction apparatus at a constant flow rate to continuously extract for a long time. That is, according to the present invention, the saponins are extracted by treating white ginseng with a pressurized heated extraction solvent, followed by cooling and recovering saponins, and further extracting saponins by further treating the pressurized heated solvent on the remaining ginseng. Can be. Such continuous extraction may be extracted by switching the temperature stepwise or continuously according to two or more preset temperature settings.

In addition, the present invention provides a saponin extract extracted by the conversion extraction method.

The saponin extract of the present invention may have a saponin content of about 100 mg to about 270 mg per 1 g of the extract, and a large amount of red ginseng-specific saponins as described above, preferably ginsenoside Rg ₃ , ginsenoside Rh _2, and PPT system. It contains.

That is, the saponin extract of the present invention may occupy about 10 to 95% of the total saponin content in the case of ginsenoside Rg ₃ which is a red ginseng-specific saponin, and about 1 to 95% of the total saponin content in the case of ginsenoside Rh ₂ . When viewed as a whole, the content of red ginseng-specific saponins may be saponin extracts that account for about 15% to about 95% of the total saponin content. The content of these saponin components can be variously controlled by changing the extraction temperature and time when using the conversion extraction method according to the present invention, by applying ethanol heated to 200 ° C. pressurized to 1MPa and saponin for 30 minutes In case of extraction, ginsenoside Rg ₃ was extracted by more than 90% of the total saponin content. Ginsenoside Rh ₂ was extracted by applying ethanol heated at 1MPa and heated to 195 ° C. for 20 minutes. Extracting about 90% more than the total saponin content was obtained. In addition, when acetic acid heated to 90 ° C. and pressurized to 1 MPa was treated with ginseng for 90 minutes and brewed vinegar (pH 2.4) for 30 minutes under a temperature condition of 120 ° C., Compound K was extracted more than about 70% of the total saponin content. To get the result.

In the embodiment of the present invention, saponin extract was prepared according to the conversion extraction method of the present invention.

On the other hand, in the test example of the present invention, the total content of the saponin extract, ginsenoside Rg ₃ , ginsenoside Rh ₂ , the compound K, PPT and PPD content of the present invention was confirmed. As a result, a large amount of saponin is extracted by the extraction method of the present invention, in particular ginsenoside Rg ₃ , ginsenoside Rh ₂ And it can be seen that the compound K is extracted in a large amount.

Therefore, the present invention ginsenoside Rg ₃ , Rh ₂ specific to red ginseng in general ginseng, such as fresh ginseng and white ginseng And a saponin conversion extraction method capable of converting saponin to compound K to produce a saponin extract containing a large amount thereof, and a saponin extract extracted by the conversion extraction method.

Below. The present invention will be described in detail by way of examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

< Example  1> Conversion Extract of Red Ginseng Specific Saponin from White Ginseng

4 years old Panax ginseng ginseng CA Meyer) was cut into appropriate size, 0.5g was put in the reactor, 5ml of each solvent (70% ethanol and 99% ethanol) was put in the reactor and pressurized to 1MPa, and then the temperature of the solvent was heated to 100 ° C using a heating device. It heated to 120 degreeC, 140 degreeC, 160 degreeC, and 180 degreeC. The pressurized heated solvents were extracted with saponins for 10, 30 and 50 minutes, respectively. The extracts were cooled / recovered and then lyophilized.

< Comparative example  1> Extraction of Saponin from White Ginseng

Saponins were extracted from white ginseng in the same manner as in Example 1 except that water was used as the solvent.

< Test Example  1> Determination of Saponin Content in Saponin Converting Extracts

<1-1> Determination of total content of 11 saponins in extract

1 g of each extract extracted in Example 1 was dissolved in 1 ml of methanol, and impurities were removed by passing through a Sep-pak column. HPLC analysis (Prevail Carbohydrate ES column (Alltech Associates, inc, 4.6x250mm, USA); mobile phase acetonitrile, isopropylalcohol, Water (HPLC Grade, JTBaker, USA); chromatogram using ELSD detector (Alltech Associates, inc, USA) Detection) to determine the total amount of saponin. Three analyzes were performed for each sample to confirm reproducibility of the results. The content was determined using 11 saponin standards (ginsenosides Rb ₁ , Rb ₂ , Rc, Rd, Re, Rf, Rg ₁ , Rg ₂ , Rg ₃ , Rh _{1 ,} Rh _{2 ,} compound K, PPT and PPD). Calibration curves were prepared and compared and quantified.

As a result, as shown in Table 1 (unit: mg / g) and Figure 2, it was found that a large amount of saponin is extracted by the conversion extraction method of the present invention.

Extraction temperature (℃) Extraction time (minutes) Extraction solvent water 70% ethanol 99% ethanol 100 10 70 98 82 30 57 131 111 50 62 98 152 120 10 96 101 102 30 79 112 126 50 94 140 137 140 10 83 123 143 30 140 159 146 50 117 267 82 160 10 319 133 116 30 207 180 148 50 155 269 159 180 10 38 185 118 30 59 139 139 50 154 156 171

<1-2> extracted Ginsenoside Rg ₃  Content measurement

Ginsenoside Rg ₃ content in the extract was measured in the same manner as in Test Example <1-1>.

The results are summarized in Table 2 (unit: mg / g extract,% in parentheses) and FIG. 3. The figures in Table 2 show the content of ginsenoside Rg ₃ , and the values in parentheses show the ratio of ginsenoside Rg ₃ to the total saponin content.

As shown in Table 2 and FIG. 3, when the extraction solvent was 99% ethanol, the extraction amount tended to increase as the extraction temperature and time were increased. , 120 ℃ and 140 ℃ (10 minutes and 30 minutes) showed a value of about 10 to 20%, but the extraction temperature is 140 ℃ (50 minutes), 160 ℃ and 180 ℃, such as temperature or time Increasing the ratio was increased to about 25 to 35% was found to have a high conversion ability. On the contrary, when the extraction solvent was made of water, the low value of about 1 mg (1%) was found, indicating that the conversion was not performed smoothly.

Extraction temperature (℃) Extraction time (minutes) Extraction solvent water 70% ethanol 99% ethanol 100 10 0.47 (0.67) 0.38 (0.39) 15.27 (18.62) 30 0 (0) 0.51 (0.39) 15.28 (13.77) 50 0.21 (0.34) 0.53 (0.54) 21.53 (14.16) 120 10 0.96 (1.00) 0.28 (0.28) 13.61 (13.34) 30 0.57 (0.72) 0.79 (0.71) 14.65 (11.63) 50 0.85 (0.90) 0.40 (0.29) 16.83 (12.28) 140 10 2.08 (2.51) 1.16 (0.94) 15.74 (11.01) 30 2.72 (1.94) 1.95 (1.23) 21.61 (14.80) 50 1.54 (1.32) 2.92 (1.10) 26.84 (32.73) 160 10 4.21 (1.32) 1.03 (0.77) 17.49 (15.08) 30 2.50 (1.21) 0 (0) 34.17 (23.09) 50 2.57 (1.66) 4.22 (1.57) 53.39 (33.58) 180 10 0 (0) 3.23 (1.75) 27.57 (23.36) 30 0 (0) 5.05 (3.63) 37.69 (27.11) 50 2.07 (1.34) 3.96 (2.54) 54.24 (31.72)

<1-3> extracted Ginsenoside Rh ₂  Content measurement

Ginsenoside Rh ₂ content in the extract was measured in the same manner as in Test Example <1-1>.

The results are summarized in Table 3 (unit: mg / g extract,% in parentheses) and FIG. 4. Table 3 shows the content of ginsenoside Rh ₂ , and the values in parentheses show the ratio of ginsenoside Rh ₂ to the total saponin content.

As shown in Table 3 and FIG. 4, when the extraction solvent was 99% ethanol, the extraction amount tended to increase as the extraction temperature and time were increased. , 120 ℃ and 140 ℃ (10 minutes and 30 minutes) showed a value of about 1 to 10%, but the extraction temperature is 140 ℃ (50 minutes), 160 ℃ and 180 ℃, such as temperature or time Increasing the ratio was found to have a high conversion ability by increasing the ratio to about 20 to 35%. On the contrary, when the extraction solvent was made of water, the low value of 0 to 5 mg (0 to 3%) was found, indicating that the conversion was not smooth.

Extraction temperature (℃) Extraction time (minutes) Extraction solvent water 70% ethanol 99% ethanol 100 10 0 (0) 0 (0) 1.32 (1.61) 30 0.20 (0.35) 0.36 (0.27) 1.72 (1.55) 50 0.94 (1.52) 0 (0) 3.92 (2.58) 120 10 0.22 (0.23) 0 (0) 4.67 (4.58) 30 0 (0) 1.29 (1.15) 8.23 (6.53) 50 0.18 (0.19) 2.12 (1.51) 8.96 (6.54) 140 10 0.30 (0.36) 1.41 (1.15) 12.98 (9.08) 30 0.46 (0.33) 5.54 (3.48) 4.59 (3.14) 50 2.31 (1.97) 6.48 (2.43) 28.51 (34.77) 160 10 5.64 (1.77) 2.91 (2.19) 24.51 (21.13) 30 3.79 (1.83) 14.44 (8.02) 37.77 (25.52) 50 5.01 (3.23) 6.92 (2.57) 37.88 (23.82) 180 10 0 (0) 8.04 (4.34) 19.14 (16.22) 30 0 (0) 12.15 (8.74) 42.03 (30.24) 50 0 (0) 28.93 (18.54) 59.43 (34.75)

Taken together, these results are shown in Table 4 (unit: mg / g extract,% in parentheses), FIGS. 5 and 6. Table 4 shows the contents of ginsenosides Rg ₃ and Rh ₂ , and the values in parentheses show the ratio of ginsenosides Rg ₃ and Rh _{2 to} the total saponin content.

As shown in Table 4 below, when the extraction solvent was 99% ethanol, the saponin extract having about 15 to 70% of red ginseng-specific saponin relative to the total saponin content was obtained. When extracted for more than 50 minutes, saponin extract with red ginseng-specific saponin content of about 70% was obtained. When the extraction solvent was 70% ethanol, good saponin conversion effect was obtained when the extraction temperature was 180 ° C.

Extraction temperature (℃) Extraction time (minutes) Extraction solvent water 70% ethanol 99% ethanol 100 10 0.47 (0.67) 0.38 (0.39) 16.59 (20.23) 30 0.20 (0.35) 0.87 (0.66) 17.00 (15.32) 50 1.15 (1.87) 0.53 (0.54) 25.45 (16.74) 120 10 1.18 (1.23) 0.28 (0.28) 18.28 (17.92) 30 0.57 (0.72) 2.08 (1.86) 22.88 (18.16) 50 1.03 (1.09) 2.52 (1.80) 25.79 (18.72) 140 10 2.38 (2.87) 2.57 (2.09) 28.72 (20.09) 30 3.18 (2.27) 7.49 (4.71) 26.20 (17.94) 50 3.85 (3.29) 9.40 (3.53) 53.35 (67.50) 160 10 10.85 (3.09) 3.94 (2.96) 42.00 (36.21) 30 6.29 (3.04) 14.44 (8.02) 71.94 (48.61) 50 7.58 (4.89) 11.14 (4.14) 91.27 (57.40) 180 10 0 (0) 11.27 (6.09) 46.71 (39.58) 30 0 (0) 17.20 (12.37) 79.72 (57.35) 50 2.07 (1.34) 32.89 (21.08) 113.67 (66.47)

Thus, using the extraction method of the present invention, ginsenoside Rg ₃ and were able to obtain a large amount of ginsenoside Rg ₃ and Rh ₂ from the extract containing Rh raw material ₂ is not contained. In particular, when extracted with 99% ethanol, ginsenosides Rg ₃ and Rh ₂ , which are red ginseng-specific saponins, are included in an amount of about 15 to about 70% or more, and thus, ginsenosides Rg ₃ and Rh ₂ are obtained at a very high level. I could see that.

< Example  2> Conversion of Red Ginseng Specific Saponins from White Ginseng

In Example 1, the higher the extraction temperature was, the better the extraction efficiency was. Extraction temperatures were 190, 200, 210, 220 and 240 ° C, respectively, and extraction times were 30, 40 and 50 minutes, respectively.

< Test Example  2> Determination of Saponin Content in Saponin Converting Extracts

The total saponin content, ginsenosides Rg ₃ and Rh ₂ of the extract extracted by conversion in Example 2 were measured as in Test Example <1-1>.

As a result, as shown in Table 5 below, ginsenoside Rg ₃ was extracted at a high concentration up to 90% of the total saponin content at 190 ° C and 40 minutes, and ginsenoside Rh ₂ was extracted at 40 ° C and 90% at 220 ° C. Could.

Extraction temperature (℃) Extraction time (minutes) Total Saponin Content (%) Ginsenoside Rg ₃ (%) of total saponin content Ginsenoside Rh ₂ (%) of total saponin content 190 30 75 85 5 40 77 90 3 50 80 88 7 200 30 72 62 26 40 75 58 23 50 79 59 29 210 30 77 25 65 50 80 19 73 50 82 20 71 220 30 76 5 87 40 79 3 90 50 80 8 83 240 30 79 25 61 40 83 30 52 50 82 37 43

< Example  3> Conversion of Red Ginseng Specific Saponins from White Ginseng

In order to extract the red ginseng-specific saponins of the PPD series, the same method as in Example 1 was used. Saponin was extracted under the conditions of 80 ° C. (Table 6), 120 ° C. (Table 7), and 140 ° C. (Table 8), using brewing vinegar as a solvent. If the acid is treated instead of alcohol, the sample may burn if it is too high, so the temperature is preferably 80 to 140 ° C. However, for more economical extraction, the temperature is preferably 120 ° C or less.

< Test Example  3> Determination of Saponin Content in Saponin Converting Extracts

The total saponin content, compound K and PPD content of the saponin extract extracted in Example 3 was measured.

As a result, as shown in Table 6, Compound K exhibited a concentration of 71% of the total saponin content after treatment with acetic acid (pH0.2) for 90 minutes at 80 ° C, and the PPD content was citric acid (pH2). .8) The concentration was 82% at 90 minutes and acetic acid (pH 0.2) at 60 minutes. Compound K showed a concentration of 70% of total saponin content when treated with vinegar (pH2.4) for 30 minutes under the temperature condition of 120 ° C, and the PPD content was 90% when treated with vinegar (pH2.4) for 90 minutes. The concentration was 82% of the saponin content.

Treatment acid Processing time (minutes) Total Saponin Content (%) Compound K (%) PPD content of total saponins (%) Brewed Vinegar (pH2.4) 10 64 30 60 30 65 35 67 60 68 40 69 90 69 42 72 Persimmon Vinegar (pH3.4) 10 69 45 77 30 70 48 78 60 75 52 79 90 75 55 78 Citric Acid (pH2.8) 10 68 42 70 30 76 54 77 60 77 59 80 90 80 58 82 Citric acid solution (pH5.0) 10 60 40 65 30 65 42 68 60 60 48 65 90 62 46 66 Acetic acid (pH0.2) 10 67 48 73 30 74 52 78 60 77 60 82 90 79 71 81 2 brewed vinegar pH (2.3) 10 65 56 71 30 70 58 76 60 75 59 77 90 72 56 74

Treatment acid Processing time (minutes) Total Saponin Content (%) Compound K (%) PPD content of total saponins (%) Brewed Vinegar (pH2.4) 10 68 62 75 30 72 70 77 60 75 60 79 90 78 61 82 Persimmon Vinegar (pH3.4) 10 68 53 71 30 72 58 74 60 70 59 73 90 75 58 72 Citric Acid (pH2.8) 10 53 31 55 30 56 34 57 60 57 42 50 90 50 40 52 Citric acid solution (pH5.0) 10 27 20 35 30 30 22 38 60 35 18 35 90 36 26 36 Acetic acid (pH0.2) 10 61 40 55 30 64 42 58 60 67 40 62 90 63 41 61 2 brewed vinegar pH (2.3) 10 68 55 72 30 70 58 76 60 75 59 77 90 72 56 74

Treatment acid Processing time (minutes) Total Saponin Content (%) Compound K (%) PPD content of total saponins (%) Brewed Vinegar (pH2.4) 10 58 45 65 30 62 58 67 60 65 43 69 90 43 21 48 Persimmon Vinegar (pH3.4) 10 48 42 61 30 52 48 64 60 35 29 43 90 28 18 34 Citric Acid (pH2.8) 10 55 34 48 30 56 35 47 60 47 27 34 90 35 18 22 Citric acid solution (pH5.0) 10 23 18 26 30 27 21 28 60 29 19 25 90 26 16 18 Acetic acid (pH0.2) 10 41 28 32 30 44 32 38 60 27 24 36 90 13 21 31 2 brewed vinegar pH (2.3) 10 48 35 52 30 51 38 46 60 35 29 37 90 34 16 25

As seen from the foregoing, saponin conversion extraction method of the present invention can not only extract the saponins effective, ginseng specific saponin of ginsenosides Rg ₃ and Rh _2, etc. in the sam is not present ginsenosides Rg ₃ and Rh ₂ , Compounds K and PPD can be produced by converting and extracting. Therefore, the extraction method of the present invention can be used not only for the purpose of easily extracting saponin as a batch (batch), but also for the purpose of preparing a more efficient and economical red ginseng-specific saponin extract, the concentration of red ginseng-specific saponins It has the advantage of being adjustable freely.

Claims (11)

delete delete delete (a) pressurizing ethanol to 1 to 10 MPa and heating to 190 to 240 ° C. to prepare a high temperature, high pressure liquid solvent; And (b) a step of extracting saponin for 30 to 50 minutes by treating ginseng with a liquid solvent of high temperature and high pressure in step (a), wherein the content of extracted ginsenoside Rg ₃ is 90% or more relative to the total saponin content A method of extracting the conversion from ginseng to red ginseng-specific saponins (a) pressurizing ethanol to 1 to 10 MPa and heating to 190 to 240 ° C. to prepare a high temperature, high pressure liquid solvent; And (b) a step of extracting saponins for 30 to 50 minutes by treating ginseng with a liquid solvent of high temperature and high pressure in step (a), wherein the content of extracted ginsenoside Rh ₂ is 90% or more relative to the total saponin content A method of extracting the conversion from ginseng to red ginseng-specific saponins delete Saponin extract extracted by the conversion extraction method of claim 4 or 5. The saponin extract of claim 7, wherein the saponin extract comprises one selected from the group consisting of ginsenoside Rg ₃ , ginsenoside Rh _2, and PPT (protopanaxatriol). The edible solvent of claim 4, wherein the extractant is selected from the group consisting of vinegar, persimmon vinegar, citric acid, acetic acid, glacial acetic acid and twice brewing vinegar. Possible acids, or edible acids selected from the group consisting of vinegar, persimmon vinegar, citric acid, citric acid, glacial acetic acid, twist brewing vinegar Method of extracting compound K (20-O-β-D-glucopyranosyl-20 (S) -protopanaxadiol) and PPD (protopanaxadiol) by treatment for 30 to 90 minutes using a mixed solvent of alcohol of 6. The method of claim 9, (a) 1 to 10 MPa of edible acid selected from the group consisting of vinegar, persimmon vinegar, citric acid, citric acid, glacial acetic acid, and twice brewing vinegar. Pressurizing and heating to 80 to 140 ℃ to prepare a high temperature, high pressure liquid solvent; (b) extracting saponin for 10 minutes to 90 minutes by treating the high-temperature, high-pressure liquid solvent of step (a) with hemp; extracted compound K (20-O-β-D-glucopyranosyl- 20 (S) -protopanaxadiol) characterized in that the content of more than 70% of the total saponin. Saponin extract extracted by the conversion extraction method of claim 9 or 10.

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