KR101224574B1 - Manufacturing method of fermentative red ginseng - Google Patents
Manufacturing method of fermentative red ginseng Download PDFInfo
- Publication number
- KR101224574B1 KR101224574B1 KR1020100024464A KR20100024464A KR101224574B1 KR 101224574 B1 KR101224574 B1 KR 101224574B1 KR 1020100024464 A KR1020100024464 A KR 1020100024464A KR 20100024464 A KR20100024464 A KR 20100024464A KR 101224574 B1 KR101224574 B1 KR 101224574B1
- Authority
- KR
- South Korea
- Prior art keywords
- ginseng
- steaming
- red ginseng
- fermented red
- hours
- Prior art date
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- A23V2250/50—Polysaccharides, gums
- A23V2250/502—Gums
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Abstract
본 발명은 발효홍삼의 제조방법을 제공한다.
본 발명의 제조방법은 통상의 9증9포의 홍삼 제조방법에 비하여 증삼공정이 온도조건만 변화시키면서 하나의 공정으로 진행되므로 홍삼제조 시 발생하는 시간 및 비용을 현저하게 줄일 수 있다.
또한 본 발명의 제조방법을 통해 제조된 발효홍삼은 희유 진세노사이드(특히 Rg3, Rh2), C-K 등의 함량이 통상의 홍삼 제조방법을 통해 제조된 발효홍삼에 비하여 비약적으로 증진되는 효과를 가진다.The present invention provides a method for producing fermented red ginseng.
The manufacturing method of the present invention can significantly reduce the time and cost incurred during the manufacture of red ginseng because the process of the red ginseng proceeds as one process only changing the temperature conditions, compared to the conventional 9 ginseng 9 red ginseng manufacturing method.
In addition, the fermented red ginseng prepared by the production method of the present invention has a rarely improved content of rare ginsenosides (particularly Rg3, Rh2), CK, and the like compared to the fermented red ginseng prepared through the conventional red ginseng manufacturing method.
Description
본 발명은 발효홍삼의 제조방법에 관한 것으로, 보다 상세하게는 희유 진세노사이드의 함량을 현저하게 증진시킬 수 있는 발효홍삼의 제조방법을 제공하는 것이다.The present invention relates to a method for producing fermented red ginseng, and more particularly, to provide a method for producing fermented red ginseng that can significantly increase the content of rare ginsenosides.
인삼의 대표적인 종의 예로는, 아시아 극동 지역 (북위 33 ~ 48 : 한국, 북만주, 러시아 일부)에 자생하며, 약효가 매우 우수한 고려인삼 (Panax ginseng C.A.Meyer); 미국, 캐나다에서 자생 및 재배하고 있는 미국삼(Panax quinquefolium L.); 중국 운남성 동남부로부터 광서성 서남부 지역에서 야생 또는 재배하고 있는 전칠삼 (Panax notoginseng F.H.Chen); 및, 일본, 중국 서남부, 네팔에 이르기까지 분포되어 있는 죽절삼 (Panax japonicus C.A.Meyer) 등이 있다.Representative species of ginseng include Korean ginseng (Panax ginseng C.A.Meyer), which grows in the Far East of Asia (North 33-48: Korea, Northern Manchuria, and parts of Russia) and has very high efficacy; Panax quinquefolium L., native and cultivated in the United States and Canada; Panax notoginseng F.H.Chen, wild or cultivated in southwestern Guangxi province from southeastern Yunnan Province of China; And Panax japonicus C.A. Meyer, which extends to Japan, southwest China, and Nepal.
인삼은 신농본초경에 상품으로 수재되어 있을 뿐만 아니라 예로부터 귀중한 보약으로 사용되어 오고 있다. 지금까지 많은 약리 실험을 통해 인삼은 스트레스에 대한 생체의 비특이적 저항성을 강화시키고 항산성 작용을 갖고 있음이 밝혀져 있다. 그 외에 고혈압의 개선, 인슐린 작용증강, 알록산 (ALLOXAN) 당뇨 마우스에서의 혈당강하효과, 흰 쥐의 간 RNA 합성, 단백질 합성, 당 및 지질대사 촉진효과, 항암효과 등이 있음이 밝혀졌다.Ginseng is not only stored as a commodity in the new agricultural plant, but has been used as a valuable medicine since ancient times. So far, many pharmacological experiments have shown that ginseng strengthens the nonspecific resistance of the body to stress and has anti - acidic action. In addition, hypertension, insulin action enhancement, hypoglycemic effect in ALLOXAN diabetic mice, liver RNA synthesis in white rats, protein synthesis, sugar and lipid metabolism promoting effect, and anti-cancer effect.
상술한 인삼의 주요 활성성분은 진세노사이드사포닌)이다. 진세노사이드류 화합물은 3가지 유형으로 나눌 수 있다. 먼저 한 종류는 올레안난(Oleanane)형 Pentacyclic Triterpene 사포닌으로 sapogenin은 Oleanonic acid이고 다른 두 종류는 Dammarane형의 Tetracyclic triterpene saponin: protopanoxadiol(PPD)와 Protopanaxatriol(PPT)이다. 프로토파나사디올(Protopanaxadiol)류 사포닌은 Ra1, Ra2, Ra3, Rb1, Rb2, Rb3, Rc, Rd, Rg3, Rg5, Rh2, Rh3, F2, C-K 등을 포함하고 프로토파나사트리올(Protopanaxatriol)류 사포닌은 Ginsenoside Re, Rf, Rg1, Rg2, Rg4, Rh1, Rh4등 이다.The main active ingredient of the ginseng described above is ginsenoside saponin). Ginsenosides compounds can be divided into three types. One is Oleanane Pentacyclic Triterpene Saponin, the sapogenin is Oleanonic acid, and the other two are Dammarane Tetracyclic triterpene saponin: protopanoxadiol (PPD) and Protopanaxatriol (PPT). Protopanaxadiols Saponins include Ra1, Ra2, Ra3, Rb1, Rb2, Rb3, Rc, Rd, Rg3, Rg5, Rh2, Rh3, F2, CK and the like and Protopanaxatriols Saponins are Ginsenoside Re, Rf, Rg1, Rg2, Rg4, Rh1, Rh4.
비록 Ginsenoside (사포닌)의 종류가 40여종에 달하지만 오직 Rg3, Rg5, Rh2, Rh3등 희유 사포닌 및 Rg2, Rg4, Rh1, Rh4 등 활성이 높은 사포닌만이 더욱 가치가 있다. 예를 들어 Rh2(Rh3 포함)은 항암, 암세포 사멸, 항혈전, 항암제 내성방지, 면역력을 증가시키고 ; Rh1(Rh4 포함) 은 항암, 간장보호, 항유리기 작용이 있으며; Rg3(Rg5 포함) 은 항혈전, 항암, 기억력 제고, 항유리기 작용이 있으며; Rg2(Rg4 포함) 은 심장보호, 기억력 향상, 항혈전, 항유리기 작용이 있다.Although there are over 40 species of Ginsenosides, only rare saponins such as Rg3, Rg5, Rh2, and Rh3 and saponins with high activity such as Rg2, Rg4, Rh1 and Rh4 are more valuable. For example, Rh2 (including Rh3) can increase anticancer, cancer cell death, antithrombosis, anticancer resistance, increase immunity ; Rh1 (including Rh4) has anticancer, hepatoprotective, antifree radical action; Rg3 (including Rg5) has antithrombotic, anticancer, memory enhancing, antifreeze action; Rg2 (including Rg4) has a cardioprotective, memory enhancing, antithrombotic, antifreeze effect.
그런데, 인삼중에 함량이 비교적 높은 것은 글리콜계 사포닌 Rb1, Rb2, Rb3, Rc, Rd 등과 Triol계 사포닌 Re, Rg1, Rf 등이 있다. 그리고 백삼은 보존하기 쉽지 않아 사람들은 현재 대부분 백삼을 홍삼으로 가공하여 보존하거나 식용하고 있다. 전통적인 홍삼가공방법은 백삼을 95~100℃하에 2시간 정도 증숙한 뒤 건조시키는 공정을 9회 반복하는 것이다. 상술한 종래의 홍삼제조방법을 통해 제조된 홍삼은 통상의 글리콜계 사포닌 또는 트리올계 사포닌을 희유 진세노사이드로 전환시키는데 어느 정도 효과가 있었지만, 이런 과정을 거쳐 생성된 희유 진세노사이드의 함량 역시 극히 미미한 수준에 불과하여 이를 제품으로 생산하는 경우 희유 진세노사이드의 효능이 제대로 발휘되기 어려운 문제가 있었다.
By the way, the relatively high content of ginseng is glycol saponins Rb1, Rb2, Rb3, Rc, Rd and Triol saponins Re, Rg1, Rf and the like. And white ginseng is not easy to preserve, and most people now process white ginseng as red ginseng to preserve or edible. Traditional red ginseng processing method is to repeat the process of steaming white ginseng under 95 ~ 100 ℃ for 2 hours and drying it 9 times. The red ginseng prepared by the above-described conventional red ginseng manufacturing method has a certain effect in converting conventional glycol-based saponins or triol-based saponins into rare ginsenosides, but the content of rare ginsenosides produced through such a process is also extremely low. There was a problem that the rare ginsenoside's efficacy is not properly exhibited when produced as a product because it is only a slight level.
본 발명은 상술한 문제를 해결하기 위하여 안출된 것으로, 본 발명의 첫번째 해결하려는 과제는 수삼 자체의 효소의 활성을 최대한 살리면서 증숙공정을 수행하여 희유 진세노사이드의 함량을 비약적으로 향상시킬 수 있는 발효홍삼의 제조방법을 제공하는 것이다.The present invention has been made in order to solve the above problems, the first problem to be solved of the present invention is to perform a steaming process while maximizing the activity of the enzyme of ginseng itself, which can dramatically improve the content of rare ginsenosides It is to provide a method for producing fermented red ginseng.
본 발명의 두번째 해결하려는 과제는 본 발명의 증숙공정의 수행 시 증숙조건을 한정하거나 특정한 물질을 첨가하여 희유 진세노사이드의 함량을 더욱 향상시킬 수 있는 발효홍삼의 제조방법을 제공하는 것이다.The second problem to be solved of the present invention is to provide a method for preparing fermented red ginseng that can further improve the content of rare ginsenosides by limiting the steaming conditions or adding a specific substance when performing the steaming process of the present invention.
본 발명의 세번째 해결하려는 과제는, 본 발명의 제조방법을 통해 제조된 발효홍삼을 포함하는 건강보조식품을 제공하는 것이다.The third problem to be solved of the present invention, to provide a health supplement comprising a fermented red ginseng prepared by the method of the present invention.
상술한 첫번째 과제를 달성하기 위하여 1) 수삼을 95 ~ 100℃에서 10 ~ 20분간 증숙하여 수삼의 사포닌 효소의 활성을 70 ~ 80% 유지하는 1차 증숙단계, 2) 상기 1차 증숙단계를 거친 수삼을 70 ~ 90℃에서 1 ~ 3시간 동안 증숙하는 2차 증숙단계, 3) 상기 2차 증숙단계를 거친 수삼을 50 ~ 60℃에서 60 ~ 90분간 증숙하는 3차 증숙단계, 4) 상기 3차 증숙단계를 수삼을 70 ~ 90℃에서 1 ~ 3시간 동안 증숙하는 4차 증숙단계, 5) 상기 4차 증숙단계를 거친 수삼을 120 ~ 140℃에서 10 ~ 20분간 증숙하여 수삼의 사포닌 효소를 불활성화시키는 5차 증숙단계, 6) 상기 5차 증숙단계를 거친 수삼을 20 ~ 30℃에서 1 ~ 5시간 건조하여 수삼 농축액을 제조하는 단계, 및 7) 상기 농축액 제조단계를 거친 수삼을 70 ~ 80℃에서 15 ~ 24시간 동안 발효시키는 단계를 포함하는 발효홍삼의 제조방법을 제공한다.In order to achieve the first task described above 1) steaming fresh ginseng at 95 ~ 100 ℃ for 10-20 minutes to maintain the activity of saponin enzyme of the ginseng 70-80%, 2) the first steaming step Second steaming step of steaming fresh ginseng at 70-90 ° C. for 1 to 3 hours, 3) Third steaming step of steaming fresh ginseng after 60-90 minutes at 50-60 ° C., 4) above 3 4th steaming step of steaming fresh ginseng for 1 ~ 3 hours at 70 ~ 90 ℃, 5) Steaming ginseng after 4th steaming step at 120 ~ 140 ℃ for 10 ~ 20 minutes 5th steaming step of inactivation, 6) drying the fresh ginseng passed through the 5th steaming step at 20 to 30 ° C. for 1 to 5 hours to produce a fresh ginseng concentrate, and 7) 70 to 80 Providing a method for producing fermented red ginseng comprising the step of fermenting at 15 ℃ for 24 hours .
본 발명의 두번째 과제를 달성하기 위하여, 상기 1) 단계는 0.1 ~ 0.15㎏/㎠의 수증기압에서 반응이 수행될 수 있다.In order to achieve the second object of the present invention, the step 1) may be carried out at a water vapor pressure of 0.1 ~ 0.15kg / ㎠.
본 발명의 바람직한 일실시예에 따르면, 상기 1) 단계 이전에 수삼을 숙신산 용매에 침전시키고 숙신산을 수삼내부에 잘 스며들게 하기 위하여 20 ~ 30분간 감압처리하는 단계를 더 포함할 수 있으며, 이 경우 상기 숙신산 용매의 농도는 0.5 ~ 1M일 수 있다.According to a preferred embodiment of the present invention, the step 1) may further comprise the step of depressurizing 20 to 30 minutes in order to precipitate the ginseng in the succinic acid solvent before the step 1) soaking the succinic acid into the fresh ginseng, in this case The concentration of the succinic acid solvent may be 0.5-1M.
본 발명의 바람직한 다른 일실시예에 따르면, 상기 1) 단계에서 0.5 ~ 2M의 DMSO를 투입할 수 있다.According to another preferred embodiment of the present invention, 0.5 ~ 2M DMSO in step 1) may be added.
본 발명의 바람직한 또 다른 일실시예에 따르면, 상기 6) 단계에서 글루코만난을 첨가할 수 있다.According to another preferred embodiment of the present invention, glucomannan may be added in step 6).
상술한 세번째 과제를 달성하기 위하여, 본 발명의 제조방법을 통해 제조된 발효홍삼 및 식품학적으로 허용가능한 식품보조 첨가제를 포함하는 건강보조식품을 제공한다.In order to achieve the third object described above, it provides a health supplement comprising a fermented red ginseng and a food supplement acceptable food additives produced through the manufacturing method of the present invention.
본 발명의 바람직한 다른 일실시예에 따르면, 상기 건강보조식품은 분말, 음료, 정제, 편, 캡슐 및 시럽으로 이루어진 군으로부터 선택되는 제형일 수 있다.
According to another preferred embodiment of the present invention, the dietary supplement may be a formulation selected from the group consisting of powders, beverages, tablets, flakes, capsules and syrups.
이하, 본 발명에 사용된 용어를 설명한다.Hereinafter, terms used in the present invention will be described.
본 명세서에서 용어 “사포닌”은 특별하게 다른 언급이 없는 한, 진세노사이드, 즉 인삼사포닌과 동일한 의미로 사용되고 있다.The term "saponin" is used herein with the same meaning as ginsenoside, ie ginseng saponin, unless otherwise specified.
용어 "희유 진세노사이드"라 함은 수삼에 포함된 진세노사이드 성분 중 효능이 우수하나 그 함량이 극히 미미한 성분을 통칭하는 것으로서 Rg3, Rg5, Rh2, Rh3 등이 이에 포함된다.The term "rare ginsenoside" refers to a component having excellent efficacy among the ginsenoside components contained in fresh ginseng but having a very low content, and includes Rg3, Rg5, Rh2, and Rh3.
기재의 편의상, 본 발명의 방법은 발효홍삼에 적용되는 것으로 본 명세서에 기재되어 있다. 그러나, 본 발명은 다양한 형태로 가공된 모든 홍삼에 적용될 수 있으며, 이는 본 발명의 범위에 포함된다는 것은 당업자에게 명확하다.For convenience of description, the method of the present invention is described herein as applied to fermented red ginseng. However, it is apparent to those skilled in the art that the present invention can be applied to all red ginseng processed in various forms, which is included in the scope of the present invention.
본 발명의 방법의 각 단계를 언급하면서 사용되는 용어 “수삼”은 홍삼을 만들기 위한 처리를 하지 않은 인삼을 의미한다. 수삼은 홍삼과 비교하여 희유 진세노사이드의 함량이 낮은 특징이 있다.As used to refer to each step of the method of the present invention, the term “ginseng” refers to ginseng that has not been treated to make red ginseng. Fresh ginseng has a lower content of rare ginsenosides than red ginseng.
본 발명에서 이용되는 수삼으로는, 공지된 다양한 수삼을 사용할 수 있으며, 고려삼(Panax ginseng), 회기삼(P.quiquefolius), 전칠삼(P. notoginseng), 죽절삼(P. japonicus), 삼엽삼(P. trifolium), 히말라야삼(P.pseudoginseng) 및 베트남삼(P. vietnamensis)을 포함하나, 이에 한정되지 않는다. 본 발명에서 이용되는 홍삼은 상기 수삼을 가공처리 하여 제조된 것이다. 바람직하게는, 본 발명에서 이용되는 수삼은 고려삼(Panaxginseng) 또는 전칠삼(P. notoginseng)이다.
As the ginseng used in the present invention, various known ginsengs can be used, and Korean ginseng (Panax ginseng), hoegisam (P.quiquefolius), Jeonchisam (P. notoginseng), bamboo shoots (P. japonicus), three leaf ginseng ( P. trifolium), Himalayan ginseng (P. pseudoginseng) and Vietnamese ginseng (P. vietnamensis), but are not limited thereto. Red ginseng used in the present invention is prepared by processing the ginseng. Preferably, the fresh ginseng used in the present invention is Panaxginseng or P. notoginseng.
본 발명의 제조방법은 통상의 9증9포의 홍삼 제조방법에 비하여 증삼공정이 온도조건만 변화시키면서 하나의 공정으로 진행되므로 홍삼제조 시 발생하는 시간 및 비용을 현저하게 줄일 수 있다.The manufacturing method of the present invention can significantly reduce the time and cost incurred during the manufacture of red ginseng because the process of the red ginseng proceeds as one process only changing the temperature conditions, compared to the conventional 9 ginseng 9 red ginseng manufacturing method.
또한 본 발명의 제조방법을 통해 제조된 발효홍삼은 희유 진세노사이드(특히 Rg3, Rh2), C-K 등의 함량이 통상의 홍삼 제조방법을 통해 제조된 발효홍삼에 비하여 비약적으로 증진되는 효과를 가진다.
In addition, the fermented red ginseng prepared by the production method of the present invention has a rarely improved content of rare ginsenosides (particularly Rg3, Rh2), CK, and the like compared to the fermented red ginseng prepared through the conventional red ginseng manufacturing method.
도 1은 본 발명의 바람직한 일실시예에 따른 발효홍삼의 HPLC 그래프이다.
도 2는 본 발명의 바람직한 일실시예에 따른 TLC 분석결과이다.1 is an HPLC graph of fermented red ginseng according to an embodiment of the present invention.
2 is a TLC analysis result according to an embodiment of the present invention.
이하, 본 발명을 보다 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
상술한 바와 같이 종래의 전통적인 9증9포의 방법으로 제조된 홍삼은 통상의 글리콜계 사포닌 또는 트리올계 사포닌을 희유 진세노사이드로 전환시키는데 어느 정도 효과가 있었지만, 이런 과정을 거쳐 생성된 희유 진세노사이드의 함량 역시 극히 미미한 수준에 불과하여 이를 제품으로 생산하는 경우 희유 진세노사이드의 효능이 제대로 발휘되기 어려운 문제가 있었다.
As described above, the red ginseng prepared by the conventional method of 9 capillary 9 sachets was somewhat effective in converting conventional glycol-based saponins or triol-based saponins into rare ginsenosides, but the rare ginsenosides produced through such a process were produced. The content of the side is also only a very small level, when producing the product there was a problem that the efficacy of rare ginsenosides difficult to be properly exhibited.
이에 본 발명에서는 1) 수삼을 95 ~ 100℃에서 10 ~ 20분간 증숙하여 수삼의 사포닌 효소의 활성을 70 ~ 80% 유지하는 1차 증숙단계, 2) 상기 1차 증숙단계를 거친 수삼을 70 ~ 90℃에서 1 ~ 3시간 동안 증숙하는 2차 증숙단계, 3) 상기 2차 증숙단계를 거친 수삼을 50 ~ 60℃에서 60 ~ 90분간 증숙하는 3차 증숙단계, 4) 상기 3차 증숙단계를 수삼을 70 ~ 90℃에서 1 ~ 3시간 동안 증숙하는 4차 증숙단계, 5) 상기 4차 증숙단계를 거친 수삼을 120 ~ 140℃에서 10 ~ 20분간 증숙하여 수삼의 사포닌 효소를 불활성화시키는 5차 증숙단계, 6) 상기 5차 증숙단계를 거친 수삼을 20 ~ 30℃에서 1 ~ 5시간 건조하여 수삼 농축액을 제조하는 단계, 및 7) 상기 농축액 제조단계를 거친 수삼을 70 ~ 80℃에서 15 ~ 24시간 동안 발효시키는 단계를 포함하는 발효홍삼의 제조방법을 제공하여 희유 진세노사이드의 함량을 비약적으로 향상시켰다.In the present invention, 1) steaming fresh ginseng at 95-100 ° C. for 10-20 minutes to maintain 70-80% of saponin enzyme activity of fresh ginseng; Second steaming step of steaming at 90 ° C. for 1 to 3 hours, 3) Third steaming step of steaming fresh ginseng through the second steaming step at 50 to 60 ° C. for 60 to 90 minutes, 4) The third steaming step 4th steaming step of steaming fresh ginseng for 1 to 3 hours at 70 to 90 ° C, 5) 5th step of inactivating saponin enzyme of fresh ginseng by steaming fresh ginseng after the 4th steaming step at 120 to 140 ° C for 10 to 20 minutes Steaming step, 6) drying the fresh ginseng after the fifth steaming step at 20 ~ 30 ℃ for 1 to 5 hours to prepare a fresh ginseng concentrate, and 7) 15 ~ at 70 ~ 80 ℃ the ginseng through the concentrate preparation step Rare Ginsenosai by providing a method for producing fermented red ginseng comprising fermentation for 24 hours The content was significantly improved.
먼저, 본 발명의 증숙단계를 설명한다. 본 발명의 증숙단계는 수삼을 수세한 후 증숙기에 넣고 열을 가하여 증숙하는 것으로서, 증숙단계의 중간에 별도의 건조단계를 거치는 것이 아니라 하나의 증숙단계에서 온도조건만을 변화시킨 것이다. 다시 말해, 증숙기 내부로 투입된 수삼은 중간에 건조하기 위하여 밖으로 빼내는 것이 아니라 증숙기 내부에서 온도조건 및 증숙시간을 4차례 바꾸어 하나의 공정으로 증숙공정이 수행되는 것으로서, 상술한 1차 증숙단계 내지 5차 증숙단계는 각각 독립적인 공정이 아니라 하나의 공정으로서 온도조건 및 증숙시간에 변화를 준 것이다.First, the steaming step of the present invention will be described. The steaming step of the present invention is to steam the fresh ginseng, and then steam by putting it in a steamer, and changing only the temperature conditions in one steaming step, not through a separate drying step in the middle of the steaming step. In other words, the fresh ginseng introduced into the steamer is not taken out to dry in the middle, but the steaming process is performed in one process by changing the temperature condition and the steaming time four times in the steamer. The 5th steaming step is not a separate process but a process that changes the temperature conditions and the steaming time.
구체적으로, 수삼을 증숙기에 넣고 1차 증숙공정을 수행한다. 보다 구체적으로 수삼이 자체적으로 보유한 사포닌 효소의 활성을 70 ~ 80% 유지하기 위하여 수삼을 95 ~ 100℃에서 10 ~ 20분간 증숙하는 것으로서, 만일 95℃ 미만으로 증숙하면 증숙효과가 미미하고, 100℃를 초과하면 사포닌 효소의 활성을 유지하기 어렵다. 또한 증숙시간이 10분 미만인 경우에는 증숙효과를 달성하기 어렵고, 20분을 초과하는 경우에는 사포닌 효소의 활성이 유지되지 않으므로 희유 진세노사이드의 함량이 현저하게 떨어지게 된다(표 1 참조).Specifically, the fresh ginseng is put into a steamer and the first steaming process is performed. More specifically, in order to maintain 70-80% of the activity of saponin enzyme possessed by fresh ginseng, steamed ginseng is steamed at 95 to 100 ° C. for 10 to 20 minutes. If it exceeds, it is difficult to maintain the activity of the saponin enzyme. In addition, if the steaming time is less than 10 minutes, it is difficult to achieve the steaming effect, and if it exceeds 20 minutes, the saponin enzyme activity is not maintained, so the rare ginsenoside content is significantly reduced (see Table 1).
상기 1차 증숙단계를 거친 수삼을 온도를 낮추어 70 ~ 90℃에서 1 ~ 3시간 동안 2차 증숙한다. 만일 2차 증숙온도가 70℃ 미만이면 증숙효과가 미미하고 90℃를 초과하면 사포닌 효소의 활성을 유지시키기 어려워진다. 또한 증숙시간이 1시간 미만이면 증숙효과가 미미하고 3시간을 초과하면 경제성이 떨어진다.The fresh ginseng, which has undergone the first steaming step, is steamed for 2 hours at a temperature of 70 to 90 ° C. for 1 to 3 hours. If the secondary steaming temperature is less than 70 ℃ steaming effect is insignificant, if it exceeds 90 ℃ it is difficult to maintain the activity of the saponin enzyme. In addition, if the steaming time is less than 1 hour, the steaming effect is insignificant, and if it exceeds 3 hours, the economic efficiency is low.
상기 2차 증숙단계를 거친 수삼을 온도를 더욱 낮추어 50 ~ 60℃에서 60 ~ 90분간 3차 증숙한다. 2차 증숙을 거친 후 온도를 더욱 낮추는 것이 희유 진세노사이드의 함량증진에 도움이 되며 50℃ 미만이면 증숙효과가 미미하고, 60℃를 초과하면 온도를 낮춘 의미가 없어지게 된다. 또한 증숙시간이 60분 미만이면 증숙효과가 미미하고 90분을 초과하면 경제성이 떨어진다. The fresh ginseng, which has undergone the second steaming step, is further steamed at 50 to 60 ° C. for 60 to 90 minutes. Lowering the temperature after the second steaming helps to increase the content of rare ginsenosides. If the temperature is lower than 50 ° C., the steaming effect is insignificant. In addition, if the steaming time is less than 60 minutes, the steaming effect is insignificant, and if it exceeds 90 minutes, economic efficiency is low.
상기 3차 증숙단계를 수삼을 70 ~ 90℃에서 1 ~ 3시간 동안 4차 증숙한다. 구체적인 조건은 2차 증숙과 동일하며 5차 증숙과정에서 효소를 불활성화 시키기 위한 예비단계로서 4차 증숙단계를 거치게 된다.In the third steaming step, the fresh ginseng is steamed for 4 hours at 70 to 90 ° C. for 1 to 3 hours. The specific conditions are the same as the second steaming, and the fourth steaming step is performed as a preliminary step to inactivate the enzyme in the fifth steaming process.
다음, 상기 4차 증숙단계를 거친 수삼을 120 ~ 140℃에서 10 ~ 20분간 증숙하여 수삼의 사포닌 효소를 불활성화시키는 5차 증숙단계를 거친다. 상기 단계는 수삼의 사포닌 효소를 불활성화시키기 위한 단계로서 증숙온도가 120℃ 미만이면 사포닌 효소의 불활성을 달성하기 어렵고, 140℃를 초과하면 수삼이 손상될 우려가 있다. 증숙시간 역시 10분 미만이면 증숙효과를 달성하기 어렵고, 20분을 초과하면 비경제적이다.Next, steamed ginseng undergoing the fourth steaming step is subjected to a fifth steaming step of inactivating saponin enzyme of ginseng by steaming for 10 to 20 minutes at 120 to 140 ° C. The above step is to inactivate the saponin enzyme of ginseng, if the steaming temperature is less than 120 ℃ difficult to achieve the inactivation of the saponin enzyme, if the temperature exceeds 140 ℃ there is a risk that the ginseng is damaged. Steaming time is also less than 10 minutes, it is difficult to achieve the steaming effect, more than 20 minutes is uneconomical.
다음 상기 5차 증숙단계를 거친 수삼을 20 ~ 30℃에서 1 ~ 5시간 건조하여 수삼 농축액을 제조하는 단계로서 통상의 건조공정 및 농축액의 제조방법에 따라 제조될 수 있다. 또한 열풍건조 또는 자연건조 모두 가능하다. 이 때 불필요한 고형분을 제거하기 위하여 글루코만난을 더 첨가하는 것도 가능하다. Next, the ginseng, which has undergone the fifth steaming step, is dried for 1 to 5 hours at 20 to 30 ° C. to prepare a ginseng concentrate, which may be prepared according to a conventional drying process and a method for preparing the concentrate. In addition, hot air drying or natural drying is possible. At this time, it is also possible to add glucomannan to remove unnecessary solids.
마지막으로 상기 건조 및 농축공정을 통해 제조된 수삼 농축액을 통상의 홍삼발효기에서 70 ~ 80℃에서 15 ~ 24시간 동안 발효시켜 발효홍삼을 제조한다. 이때 사용될 수 있는 발효기의 종류에는 제한이 없으며 바람직하게는 수삼 농축액에 직접 열을 가하는 것이 아니라 파이프에 수증기를 통과시켜 온도를 유지하는 간접가열방식에 의해 발효홍삼을 제조할 수 있다. Finally, the red ginseng concentrate prepared by the drying and concentration process is fermented at 70 to 80 ° C. for 15 to 24 hours in a conventional red ginseng fermenter to prepare fermented red ginseng. At this time, there is no restriction on the type of fermenter that can be used, preferably fermented red ginseng may be prepared by indirect heating method that maintains the temperature by passing steam through the pipe rather than applying heat directly to the ginseng concentrate.
본 발명의 제조방법은 통상의 9증9포의 홍삼 제조방법에 비하여 증삼공정이 온도조건만 변화시키면서 하나의 공정으로 진행되므로 홍삼제조 시 발생하는 시간 및 비용을 현저하게 줄일 수 있다. 또한 본 발명의 제조방법을 통해 제조된 발효홍삼은 희유 진세노사이드(특히 Rg3, Rh2), C-K 등의 함량이 통상의 홍삼 제조방법을 통해 제조된 발효홍삼에 비하여 비약적으로 증진되는 효과를 가진다.
The manufacturing method of the present invention can significantly reduce the time and cost incurred during the manufacture of red ginseng because the process of the red ginseng proceeds as one process only changing the temperature conditions, compared to the conventional 9 ginseng 9 red ginseng manufacturing method. In addition, the fermented red ginseng prepared by the production method of the present invention has a rarely improved content of rare ginsenosides (particularly Rg3, Rh2), CK, and the like compared to the fermented red ginseng prepared through the conventional red ginseng manufacturing method.
한편, 상기 1차 증숙단계 이전에 수삼을 숙신산 용매에 침전시켜 희유 진세노사이드의 함량을 더욱 증진시킬 수 있다. 이 경우 숙신산을 수삼내부에 잘 스며들게 하기 위하여 20 ~ 30분간 감압처리하는 단계를 더 포함할 수 있다. 이 때 감압조건은 10 ~ 30℃에서 진공도 50 ~ 200mmHg의 감압조건을 통해 감압을 수행할 있으며 감압수단은 통상의 감압기를 이용하거나 플라스크 상에서 압력을 감소시켜 감압공정을 수행할 수 있으며, 이 경우 상기 숙신산 용매의 농도는 0.5 ~ 1M일 수 있으나 이에 제한되는 것은 아니다. 또한, 본 발명에서는 숙신산 용매에 수삼을 침전시켰으며, 숙신산이 아닌 다른종류의 유기산 예를 들어 시트르산, 구연산으로 수삼을 처리하는 경우 숙신산보다 희유 진세노사이드의 함량증진 효과가 현저하게 떨어지는 단점이 있다(표 1 참조).
Meanwhile, before the first steaming step, the fresh ginseng may be precipitated in the succinic acid solvent to further increase the rare ginsenoside content. In this case, the succinic acid may further include a step of decompression for 20 to 30 minutes in order to soak well into the ginseng. At this time, the depressurization condition may be carried out through the decompression conditions of the vacuum degree of 50 ~ 200mmHg at 10 ~ 30 ℃ and the decompression means can perform a decompression process by using a conventional pressure reducer or reducing the pressure on the flask, in this case The concentration of the succinic acid solvent may be 0.5 to 1M, but is not limited thereto. In addition, the present invention precipitated ginseng in the succinic acid solvent, there is a disadvantage that the content enhancement effect of rare ginsenosides significantly lower than succinic acid when the ginseng is treated with other types of organic acids other than succinic acid, such as citric acid and citric acid. (See Table 1).
이에 더하여, 상기 1차 증숙단계는 0.1 ~ 0.15㎏/㎠의 수증기압에서 반응을 수행하여 희유 진세노사이드의 함량을 더욱 증진시킬 수 있다(표 1 참조). 이를 위하여 상기 증숙단계는 고압 증숙기에서 수행될 수 있으며, 만일 수증기압이 0.1㎏/㎠ 미만이면 희유 진세노사이드의 함량증진 효과가 미미하고, 0.15㎏/㎠를 초과하는 경우에도 희유 진세노사이드의 함량증진 효과가 거의 발생하지 않게된다.
In addition, the first steaming step may further enhance the rare ginsenoside content by performing the reaction at a water vapor pressure of 0.1 ~ 0.15kg / ㎠ (see Table 1). To this end, the steaming step may be performed in a high-pressure steamer, and if the water vapor pressure is less than 0.1 kg / cm 2, the effect of increasing the content of rare ginsenosides is insignificant, even if it exceeds 0.15 kg / cm 2. The content enhancing effect hardly occurs.
나아가, 상기 1차 증숙단계에서 희유 진세노사이드의 함량을 더욱 증진시키기 위하여 0.5 ~ 2M의 DMSO(Dimethyl Sulfoxide)를 투입할 수 있다. 이 경우 증숙되는 수삼 100중량부에 대하여 1 ~ 3중량부를 첨가할 수 있으나 이에 한정되는 것은 아니며, DMSO의 첨가를 통해 희유 진세노사이드의 함량의 증진을 도모할 수 있다(표 1 참조). 만일 DMSO의 함량이 0.5M 미만이면 첨가효과가 미미하고 2M을 초과하면 경제성이 떨어지는 단점을 가진다.Furthermore, in order to further enhance the rare ginsenoside content in the first steaming step, 0.5 to 2 M of DMSO (dimethyl sulfate) may be added. In this case, 1 to 3 parts by weight may be added to 100 parts by weight of steamed ginseng, but the present invention is not limited thereto. The addition of DMSO can promote the content of rare ginsenosides (see Table 1). If the content of DMSO is less than 0.5M, the effect of addition is insignificant, and if it exceeds 2M, it is disadvantageous in economy.
결국, 상술한 조건을 포함하여 제조된 발효홍삼은 희유 진세노사이드의 함량이 많아지며, 상술한 모든 조건을 동시에 만족하는 경우 희유 진세노사이드의 함량이 현저하게 증진하게 되는 것이다.
As a result, the fermented red ginseng prepared with the above-mentioned conditions increases the rare ginsenoside content, and when the above-mentioned conditions are satisfied at the same time, the rare ginsenoside content is remarkably enhanced.
상술한 방법을 통해 제조된 발효홍삼 및 식품학적으로 허용가능한 식품보조 첨가제를 포함하여 건강보조식품을 제조할 수 있으며, 상기 건강보조식품은 통상적인 분말, 음료, 정제, 편, 캡슐 및 시럽으로 이루어진 군으로부터 선택되는 제형일 수 있다.Fermented red ginseng prepared by the above-described method and food supplements can be prepared health supplements including food acceptable additives, the dietary supplement is made of conventional powders, beverages, tablets, flakes, capsules and syrups It may be a formulation selected from the group.
상기 건강보조식품은 바람직하게는 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산 음료에 사용되는 탄산화제 등을 함유할 수 있다.
The dietary supplement is preferably various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like.
이하, 실시예를 통하여 본 발명을 더욱 구체적으로 설명하기로 하지만, 하기 실시예가 본 발명의 범위를 제한하는 것은 아니며, 이는 본 발명의 이해를 돕기 위한 것으로 해석되어야 할 것이다.
Hereinafter, the present invention will be described in more detail with reference to Examples, but the following Examples are not intended to limit the scope of the present invention, which will be construed as to help the understanding of the present invention.
<실시예 1>≪ Example 1 >
신선한 수삼을 취하여 세척한 후 고압 증숙기에 넣고 95℃에서 10분간 증숙하였다. 그 뒤 온도를 70℃로 낮추어 2시간 정도 증숙한 후 온도를 50℃로 내려 70분간 반응시키고 다시 70℃에서 2시간 반응시킨 후 온도를 130℃로 올리고 20분간 증심을 실시하고 이를 건조하여 발효홍삼 원료를 얻었다. 수득한 발효된 홍삼원료를 90℃에서 8배수에 물을 가하여 8시간에 1회씩 3회 반복 추출하고 40℃ 이하에서 30 브릭스(brix)까지 저온 농축하였다. 상기 저온농축한 홍삼추출물을 5℃로 냉각하고 16,000rpm으로 원심 분리하여 침전물을 제거하고 이를 발효챔버에 넣은 후 80℃에서 20시간 정도 발효시켜 발효홍삼을 수득하였다.
Fresh ginseng was taken, washed, put in a high pressure steamer and steamed at 95 ° C for 10 minutes. After lowering the temperature to 70 ℃ and steaming for about 2 hours, the temperature was lowered to 50 ℃ and reacted for 70 minutes, then reacted again at 70 ℃ for 2 hours, the temperature was raised to 130 ℃ and 20 minutes and dried and fermented red ginseng Raw material was obtained. The obtained fermented red ginseng raw material was repeatedly extracted three times once every 8 hours by adding water to 8-fold water at 90 ° C., and then concentrated at low temperature to 30 brix at 40 ° C. or lower. The cold concentrated red ginseng extract was cooled to 5 ° C. and centrifuged at 16,000 rpm to remove the precipitate, which was then fermented at 80 ° C. for 20 hours to obtain fermented red ginseng.
<실시예 2><Example 2>
신선한 수삼을 세척한 후 증숙기에 넣기 전에 1.0M의 숙신산 용매에 침전시키고 200mmHg에서 감압처리한 후 증숙기에 넣은 것을 제외하고는 실시예 1과 동일하게 실시하여 발효홍삼을 수득하였다.
After washing fresh ginseng, it was precipitated in 1.0M succinic acid solvent before being put into a steamer, and subjected to the same procedure as in Example 1 except that it was subjected to reduced pressure at 200mmHg and put into a steamer to obtain fermented red ginseng.
<실시예 3><Example 3>
최초 95℃에서 10분간 증숙하는 단계는 고압 증숙기의 수증기압을 0.1 ㎏/㎠를 유지한 상태에서 반응을 수행한 것을 제외하고는 실시예 1과 동일하게 실시하여 발효홍삼을 수득하였다.
The first step of steaming at 95 ° C. for 10 minutes was carried out in the same manner as in Example 1 except that the reaction was carried out while maintaining the steam pressure of the high pressure steamer at 0.1 kg / cm 2, thereby obtaining fermented red ginseng.
<실시예 4><Example 4>
최초 95℃에서 10분간 증숙하는 단계에서 1M의 DMSO 1ℓ를 투입한 것을 제외하고는 실시예 1과 동일하게 실시하여 발효홍삼을 수득하였다.
Fermented red ginseng was obtained in the same manner as in Example 1 except that 1 L of 1M DMSO was added at a step of steaming for 10 minutes at 95 ° C.
<실시예 5><Example 5>
신선한 수삼을 세척한 후 1.0M의 숙신산 용매에 침전시키고 200mmHg에서 감압처리하였다. 그 뒤 고압 증숙기에 넣고 수증기압을 0.1 ㎏/㎠를 유지한 상태에서 95℃에서 10분간 증숙하였다. 이 때 1M의 DMSO를 고압 증숙기에 투입하였다. 이후 수증기압을 제거하고 온도를 70℃로 낮추어 2시간 정도 증숙한 후 온도를 50℃로 내려 70분간 반응시키고 다시 70℃에서 2시간 반응시킨 후 온도를 130℃로 올리고 20분간 증심을 실시하고 이를 건조하여 발효홍삼 원료를 얻었다. 수득한 발효된 홍삼원료를 90℃에서 8배수에 물을 가하여 8시간에 1회씩 3회 반복 추출하고 40℃ 이하에서 30 브릭스(brix)까지 저온 농축하였다. 상기 저온농축한 홍삼추출물을 5℃로 냉각하고 16,000rpm으로 원심 분리하여 침전물을 제거하고 이를 발효챔버에 넣은 후 80℃에서 20시간 정도 발효시켜 발효홍삼을 수득하였다.
Fresh ginseng was washed and then precipitated in 1.0M succinic acid solvent and reduced pressure at 200mmHg. Thereafter, the resultant was placed in a high pressure steamer and steamed at 95 ° C. for 10 minutes while maintaining a water vapor pressure of 0.1 kg / cm 2. At this time, 1M of DMSO was put in a high pressure steamer. After removing the steam pressure and lowering the temperature to 70 ℃ and steamed for 2 hours, the temperature was lowered to 50 ℃ and reacted for 70 minutes, and then reacted again at 70 ℃ for 2 hours, the temperature was raised to 130 ℃ and subjected to steaming for 20 minutes and dried To obtain a fermented red ginseng raw material. The obtained fermented red ginseng raw material was repeatedly extracted three times once every 8 hours by adding water to 8-fold water at 90 ° C., and then concentrated at low temperature to 30 brix at 40 ° C. or lower. The cold concentrated red ginseng extract was cooled to 5 ° C. and centrifuged at 16,000 rpm to remove the precipitate, which was then fermented at 80 ° C. for 20 hours to obtain fermented red ginseng.
<비교예 1>≪ Comparative Example 1 &
통상의 9증9포의 방법으로 발효홍삼을 제조하였다. 구체적으로 신선한 수삼을 취하여 세척한 후 증숙기에 넣고 110℃에서 3시간 증숙하고, 다시 40℃에서 3시간 열풍건조한 후, 이를 8회 반복하여 발효홍삼 원료를 얻었다. 수득한 발효된 홍삼원료를 90℃에서 8배수에 물을 가하여 8시간에 1회씩 3회 반복 추출하고 40℃ 이하에서 30 브릭스(brix)까지 저온 농축하였다. 상기 저온농축한 홍삼추출물을 5℃로 냉각하고 16,000rpm으로 원심 분리하여 침전물을 제거하고 이를 발효챔버에 넣은 후 80℃에서 20시간 정도 발효시켜 발효홍삼을 수득하였다.
Fermented red ginseng was prepared by the conventional method of 9, 9 sachets. Specifically, fresh ginseng was taken, washed, put in a steamer, steamed at 110 ° C. for 3 hours, dried again at 40 ° C. for 3 hours, and then repeated eight times to obtain fermented red ginseng raw material. The obtained fermented red ginseng raw material was repeatedly extracted three times once every 8 hours by adding water to 8-fold water at 90 ° C., and then concentrated at low temperature to 30 brix at 40 ° C. or lower. The cold concentrated red ginseng extract was cooled to 5 ° C. and centrifuged at 16,000 rpm to remove the precipitate, which was then fermented at 80 ° C. for 20 hours to obtain fermented red ginseng.
<비교예 2>Comparative Example 2
최초 95℃에서 2시간 동안 증숙한 것을 제외하고는 실시예 1과 동일하게 실시하여 발효홍삼을 수득하였다.
Fermented red ginseng was obtained in the same manner as in Example 1 except that the sample was steamed at 95 ° C. for 2 hours.
<비교예 3>≪ Comparative Example 3 &
1.0M의 숙신산 용매가 아닌 1.0M의 구연산 용매를 사용한 것을 제외하고는 실시예 2와 동일하게 실시하여 발효홍삼을 수득하였다.
Fermented red ginseng was obtained in the same manner as in Example 2 except that 1.0M citric acid solvent was used instead of 1.0M succinic acid solvent.
<비교예 4>≪ Comparative Example 4 &
수증기압을 0.25 ㎏/㎠로 유지한 것을 제외하고는 실시예 3과 동일하게 실시하여 발효홍삼을 수득하였다.Fermented red ginseng was obtained in the same manner as in Example 3 except that the steam pressure was maintained at 0.25 kg / cm 2.
<실험예><Experimental Example>
실시예 1 ~ 5와 비교예 1 ~ 4에서 수득한 발효홍삼에 대하여 HPLC를 실시하여 진세노사이드의 함량을 분석하여 표 1에 나타내었다. 구체적인 HPLC의 조건은 HPLC 분석 조건으로는 Agilent 1100 series HPLC 시스템(독일 Agilent 사)을 사용하였고 컬럼은 Agilent XDB-C18(4.6×150mm,5micron), 컬럼의 오븐 온도는 30℃, 샘플의 로딩양은 20㎕로 하였다. 이동상으로는 20% 아세토니트릴에서 80%아세토니트릴을 135분간 농도구배하였다. 유속은 분당 1ml로 하였으며 컬럼 세척은 100℃ 아세토니트릴로 10분간 세척하였고 203nm에서 검출하였다
The fermented red ginseng obtained in Examples 1 to 5 and Comparative Examples 1 to 4 was subjected to HPLC to analyze the content of ginsenosides. Specific HPLC conditions were Agilent 1100 series HPLC system (Agilent, Germany) as the HPLC analysis conditions, the column was Agilent XDB-C18 (4.6 × 150mm, 5micron), the oven temperature of the column 30 ℃, the amount of sample loading 20 Μl. The mobile phase was gradient gradient of 20% acetonitrile and 80% acetonitrile for 135 minutes. The flow rate was 1 ml per minute and the column was washed with 100 ° C acetonitrile for 10 minutes and detected at 203 nm.
단, 표 1에서 C-K 는 사포닌 C와 K의 합을 의미하며, "+"는 극미량을 의미한다.In Table 1, C-K means the sum of saponin C and K, and "+" means a trace amount.
표 1에서 알 수 있듯이, 통상의 9증9포의 방법으로 발효홍삼을 제조한 비교예 1에 비하여 본 발명의 실시예 1의 발효홍삼이 희유 진세노사이드(Rg)의 전환능이 현저하게 우수한 것을 확인할 수 있다. 구체적으로 최초 증숙단계에서 증숙시간을 줄여 수삼 자체효소의 활성을 유지한 실시예 1이 증숙시간을 늘린 비교예 2에 비하여 Rg3,Rg4 및 C-K의 함량이 현저하게 증가하였다. As can be seen from Table 1, the fermented red ginseng of Example 1 of the present invention is significantly superior in the conversion ability of rare ginsenosides (Rg) compared to Comparative Example 1 of the fermented red ginseng prepared by the conventional method of 9, 9 sachets. You can check it. Specifically, the content of Rg3, Rg4 and C-K was significantly increased compared to Comparative Example 2 in which the steaming time was reduced in the first steaming step to maintain the activity of the ginseng self-enzyme.
또한, 증숙과정 이전에 숙신산으로 처리한 실시예 2는 이를 처리하지 않은 실시예 1에 비하여 Rg2, Rh2 및 Rg3의 함량이 2 ~ 3배이상 개선되었다. 그러나 숙신산이 아닌 구연산으로 처리한 비교예 3은 실시예 2에 비하여 Rg3의 함량이 1/2 수준에 불과하였다. 나아가, 최초 증숙단계에서 0.1 ~ 0.15㎏/㎠의 수증기압을 부가한 실시예 3은 실시예 1에 비하여 Rg3, Rh2 및 C-K의 함량이 현저하게 증가하였으나, 0.25㎏/㎠를 부가한 비교예 4는 수증기압을 부가하지 않은 실시예 1에 비하여 Rg3의 함량은 상승하였지만 실시예 3에 비하여는 함량이 미달하였고 C-K의 함량은 오히려 떨어진 것을 확인할 수 있다. In addition, Example 2 treated with succinic acid before the steaming process has improved the content of Rg2, Rh2 and Rg3 more than 2-3 times compared to Example 1 not treated with this. However, in Comparative Example 3 treated with citric acid instead of succinic acid, the content of Rg3 was only 1/2 level as compared with Example 2. Furthermore, in Example 3, in which the steam pressure of 0.1 to 0.15 kg / cm 2 was added in the initial steaming step, the contents of Rg3, Rh2 and CK increased significantly compared to Example 1, but Comparative Example 4, which added 0.25 kg / cm 2, Compared to Example 1 without adding the steam pressure, the content of Rg3 was increased, but the content was less than that of Example 3, and the content of CK was rather decreased.
또한 DMSO를 첨가한 실시예 4가 이를 첨가하지 않은 실시예 1에 비하여 희유진세노사이드의 전환능이 현저하게 우수하였다.In addition, Example 4 with DMSO was remarkably superior in conversion ability of rare jinsenosides compared to Example 1 without addition.
한편, 실시예 1 ~ 4의 모든 구성요소를 채용한 실시예 5의 희유 진세노사이드의 함량이 이들 실시예 1 ~ 4에 비하여 현저하게 증진된 것을 확인할 수 있다(도 1, 2 참조).
On the other hand, it can be seen that the content of the rare ginsenoside of Example 5, which employs all the components of Examples 1 to 4, is significantly improved compared to those of Examples 1 to 4 (see FIGS. 1 and 2).
본 발명의 발효홍삼제조방법을 통해 제조된 발효홍삼은 희유 진세노사이드의 함량이 일반 발효홍삼에 비하여 현저하게 우수하므로 건강식품산업에 대단히 유용하다.
Fermented red ginseng prepared by the fermented red ginseng manufacturing method of the present invention is very useful in the health food industry because the content of rare ginsenosides is significantly superior to the general fermented red ginseng.
Claims (9)
2) 상기 1차 증숙단계를 거친 수삼을 70 ~ 90℃에서 1 ~ 3시간 동안 증숙하는 2차 증숙단계;
3) 상기 2차 증숙단계를 거친 수삼을 50 ~ 60℃에서 60 ~ 90분간 증숙하는 3차 증숙단계;
4) 상기 3차 증숙단계를 수삼을 70 ~ 90℃에서 1 ~ 3시간 동안 증숙하는 4차 증숙단계;
5) 상기 4차 증숙단계를 거친 수삼을 120 ~ 140℃에서 10 ~ 20분간 증숙하여 수삼의 사포닌 효소를 불활성화시키는 5차 증숙단계;
6) 상기 5차 증숙단계를 거친 수삼을 20 ~ 30℃에서 1 ~ 5시간 건조하여 수삼 농축액을 제조하는 단계; 및
7) 상기 농축액 제조단계를 거친 수삼을 70 ~ 80℃에서 15 ~ 24시간 동안 발효시키는 단계를 포함하는 발효홍삼의 제조방법.1) the first steaming step of maintaining the activity of saponin enzyme 70-80% of steamed ginseng by steaming the fresh ginseng at 95 ~ 100 ℃ 0.5 ~ 2M DMSO for 10 to 20 minutes;
2) a second steaming step of steaming fresh ginseng after the first steaming step at 70 to 90 ° C. for 1 to 3 hours;
3) a third steaming step of steaming the fresh ginseng passed through the second steaming step at 50 to 60 ° C. for 60 to 90 minutes;
4) a fourth steaming step in which the third steaming step is steamed for 1 to 3 hours at 70-90 ° C .;
5) a fifth steaming step of inactivating saponin enzyme of fresh ginseng by steaming the fresh ginseng after the fourth steaming step at 120 to 140 ° C. for 10 to 20 minutes;
6) preparing a fresh ginseng concentrate by drying the fresh ginseng passed through the fifth steaming step at 20 to 30 ° C. for 1 to 5 hours; And
7) A method for producing fermented red ginseng, comprising the step of fermenting ginseng that has undergone the concentrate preparation step at 70-80 ° C. for 15-24 hours.
상기 1) 단계는 0.1 ~ 0.15㎏/㎠의 수증기압에서 반응이 수행되는 것을 특징으로 하는 발효홍삼의 제조방법.The method of claim 1,
Step 1) is a method of producing fermented red ginseng, characterized in that the reaction is carried out at the water vapor pressure of 0.1 ~ 0.15kg / ㎠.
상기 1) 단계 이전에 수삼을 숙신산 용매에 침전시키고 숙신산을 수삼내부에 잘 스며들게 하기 위하여 20 ~ 30분간 감압처리하는 단계를 더 포함하는 것을 특징으로 하는 발효홍삼의 제조방법.The method of claim 1,
The method of producing fermented red ginseng, further comprising the step of depressurizing 20 to 30 minutes in order to precipitate the ginseng in the succinic acid solvent before the step 1) and infiltrate the succinic acid into the fresh ginseng.
상기 숙신산 용매의 농도는 0.5 ~ 1M인 것을 특징으로 하는 발효홍삼의 제조방법.The method of claim 3,
The concentration of the succinic acid solvent is a method of producing fermented red ginseng, characterized in that 0.5 ~ 1M.
상기 6) 단계에서 글루코만난을 첨가하는 것을 특징으로 하는 발효홍삼의 제조방법.The method of claim 1,
Method for producing fermented red ginseng, characterized in that the glucomannan is added in the step 6).
상기 건강보조식품은 분말, 음료, 정제, 편, 캡슐 및 시럽으로 이루어진 군으로부터 선택되는 제형인 것을 특징으로 하는 건강보조식품.9. The method of claim 8,
The dietary supplement is a dietary supplement, characterized in that the formulation selected from the group consisting of powders, beverages, tablets, flakes, capsules and syrups.
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KR20060132212A (en) * | 2005-06-17 | 2006-12-21 | (주)김가고려인삼 | Aged concentrate of ginseng steamed red, and a preparation method thereof |
KR100600795B1 (en) | 2006-01-18 | 2006-07-19 | (주) 흑삼코리아 | A black ginseng having excel content of ginsenoside rg3 ingredient and the concentrate of black ginseng |
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