KR102348283B1 - 멀티앱타머 표적 검출 - Google Patents

멀티앱타머 표적 검출 Download PDF

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KR102348283B1
KR102348283B1 KR1020167006262A KR20167006262A KR102348283B1 KR 102348283 B1 KR102348283 B1 KR 102348283B1 KR 1020167006262 A KR1020167006262 A KR 1020167006262A KR 20167006262 A KR20167006262 A KR 20167006262A KR 102348283 B1 KR102348283 B1 KR 102348283B1
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aptamer
deoxyuridine
target
complex
carboxyamide
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KR20160058777A (ko
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우르스 에이. 옥스너
루이스 에스. 그린
래리 골드
네보쟈 야닉
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소마로직, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
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KR1020167006262A 2013-09-24 2014-09-24 멀티앱타머 표적 검출 Active KR102348283B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361881629P 2013-09-24 2013-09-24
US61/881,629 2013-09-24
PCT/US2014/057143 WO2015048084A1 (en) 2013-09-24 2014-09-24 Multiaptamer target detection

Publications (2)

Publication Number Publication Date
KR20160058777A KR20160058777A (ko) 2016-05-25
KR102348283B1 true KR102348283B1 (ko) 2022-01-10

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US (2) US9926566B2 (cg-RX-API-DMAC7.html)
EP (1) EP3049523B1 (cg-RX-API-DMAC7.html)
JP (1) JP6506768B2 (cg-RX-API-DMAC7.html)
KR (1) KR102348283B1 (cg-RX-API-DMAC7.html)
CN (2) CN105555957A (cg-RX-API-DMAC7.html)
AU (1) AU2014326975B2 (cg-RX-API-DMAC7.html)
BR (1) BR112016005957A2 (cg-RX-API-DMAC7.html)
CA (1) CA2924987C (cg-RX-API-DMAC7.html)
HK (1) HK1218765A1 (cg-RX-API-DMAC7.html)
IL (1) IL244284B (cg-RX-API-DMAC7.html)
MX (1) MX361451B (cg-RX-API-DMAC7.html)
SG (1) SG11201602063UA (cg-RX-API-DMAC7.html)
WO (1) WO2015048084A1 (cg-RX-API-DMAC7.html)

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WO2017218881A1 (en) * 2016-06-17 2017-12-21 Proximity Biosciences, Llc Method for aptamer pair selection
US10927380B2 (en) * 2016-07-01 2021-02-23 Somalogic, Inc. Oligonucleotides comprising modified nucleosides
JP2020514746A (ja) 2016-12-01 2020-05-21 ノーティラス バイオテクノロジー インコーポレイテッド タンパク質をアッセイする方法
WO2019103131A1 (ja) * 2017-11-24 2019-05-31 国立大学法人 東京大学 アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法
CA3086915A1 (en) 2017-12-29 2019-07-04 Sujal M. Patel Decoding approaches for protein identification
US20210247387A1 (en) * 2018-06-22 2021-08-12 Somalogic, Inc. Improved proteomic multiplex assays
CA3182266A1 (en) 2020-06-11 2021-12-16 Vadim Lobanov Methods and systems for computational decoding of biological, chemical, and physical entities
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
US20220227890A1 (en) 2021-01-21 2022-07-21 Nautilus Biotechnology, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
EP4399528A1 (en) 2021-09-09 2024-07-17 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
EP4405683A1 (en) 2021-09-22 2024-07-31 Nautilus Subsidiary, Inc. Methods and systems for determining polypeptide interactions
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
EP4441503A1 (en) 2021-11-30 2024-10-09 Nautilus Subsidiary, Inc. Particle-based isolation of proteins and other analytes
CA3245990A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. INTEGRATED NETWORKS FOR SINGLE-ANALYTE PROCESSES
EP4515026A1 (en) 2022-04-25 2025-03-05 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
US20240183858A1 (en) 2022-11-15 2024-06-06 Nautilus Subsidiary, Inc. Standard polypeptides
US20240192202A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. Fluidic media for single-analtye arrays
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
US20240241110A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
EP4689173A1 (en) 2023-03-24 2026-02-11 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
WO2024216233A1 (en) 2023-04-13 2024-10-17 Nautilus Subsidiary, Inc. Artificial proteins for displaying epitopes
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
US20250205706A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
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Also Published As

Publication number Publication date
EP3049523A4 (en) 2017-05-17
MX361451B (es) 2018-12-06
CN105555957A (zh) 2016-05-04
JP2016531593A (ja) 2016-10-13
AU2014326975A2 (en) 2017-01-05
AU2014326975A1 (en) 2016-04-07
WO2015048084A1 (en) 2015-04-02
CA2924987C (en) 2022-08-16
SG11201602063UA (en) 2016-04-28
IL244284A0 (en) 2016-04-21
KR20160058777A (ko) 2016-05-25
US9926566B2 (en) 2018-03-27
JP6506768B2 (ja) 2019-04-24
HK1218765A1 (zh) 2017-03-10
US10392621B2 (en) 2019-08-27
CA2924987A1 (en) 2015-04-02
BR112016005957A2 (pt) 2017-09-26
EP3049523B1 (en) 2019-08-07
CN114703193A (zh) 2022-07-05
MX2016003320A (es) 2016-05-31
EP3049523A1 (en) 2016-08-03
US20180187197A1 (en) 2018-07-05
IL244284B (en) 2019-12-31
US20160237435A1 (en) 2016-08-18
AU2014326975B2 (en) 2020-05-07

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