KR102331532B1 - Method to decrease mortality rate of trout on acclimation to seawater - Google Patents

Abstract

The present invention relates to a method for decreasing a trout mortality rate during seawater acclimation. According to the present invention, early brine acclimation is performed on young individuals of less than 50 g, and the method is improved in growth rate as compared with existing fish farming methods. The method includes: a step of growing a fertilized trout fry to a fish weight of 10 to 30 g; a first acclimation step of acclimating the grown fry at low salinity; first and second salinity raising steps of increasing salinity after the first acclimation step is completed; a second acclimation step of maintaining salinity after the second salinity raising step is completed; and a third salinity raising step of raising salinity to the salinity of seawater after the second acclimation step.

Description

Method to decrease mortality rate of trout on acclimation to seawater

The present invention relates to a method for reducing the mortality rate in the process of acclimatization of trout, and more particularly, the process of acclimatization of trout with an improved growth rate compared to conventional aquaculture methods by acclimatizing young individuals less than 50 g to salt water at an early stage. It relates to a method for reducing mortality in

Trout are cold-water, and live only in cold, clean first-class water. After hatching, they live in rivers, go down to the sea, live, become mothers, return to rivers, lay eggs, and end their lives. Originally, salmon have physiological adaptation to seawater, so they can be bred in the sea after a certain period of acclimatization. Due to these characteristics, trout farming using seawater became possible naturally, but for the implementation of trout farming in seawater, acclimatization of seawater must be carried out.

This seawater acclimatization process is a step in which the fish fry are moved into the breeding tank and the salinity of the water in the breeding tank is continuously increased by using the osmotic pressure control ability of the fish to adapt to survive in the salinity corresponding to the seawater.

In the case of freshwater fish, body fluid has a higher salinity than environmental water, so water continuously penetrates into the body through the gills, digestive organs, and epidermis. It keeps the body hydrated. At this time, monovalent ions, such as Na+, excreted with urine are absorbed by tissue epithelium such as kidney and bladder, and salts contained in food are absorbed by intestinal epithelial cells. In addition, there are salt cells of the fresh water type in the gill stool, which absorbs the salt by the action of proton ATPase enzyme on the vacuole to maintain the salinity of the body fluid. In the case of seawater fish, body fluid has a lower salinity than environmental water, so to prevent physiological dehydration, a large amount of seawater is introduced into the body to absorb moisture from the intestine.

Trout belonging to the salmon family are biologically hatched in freshwater, grow into fry, and then gradually salinize in brackish water and undergo Seawater acclimation. Therefore, it is necessary to supply fresh water from the onshore tank, brackish water from a sanctuary installed on land or sea space, and seawater from a sea cage.

Since moving trout used to freshwater to the sea places a considerable burden on the fish body, it is appropriate to raise the fish body in a state that is healthy and tolerant to changes in the environment and then move it to sea water. (On land) After about a year of being raised, the weight is usually around 200-350g.

Therefore, in normal inland fish farms, medium-sized fish weighing around 200 g are purchased, acclimatized to seawater for a certain period of time, stocked in marine cages or onshore tank farms, nurtured for 6-7 months, and released as adult fish with an average size of 2 kg. Shipment size varies greatly by region, ranging from 900g to 3kg, due to differences in water temperature, stocking size, and shipping time by region, and differences in aquaculture technology also act as a factor.

However, after breeding in fresh water at around 150~200g, it requires considerable logistical costs to move back to the sea for acclimatization. The seawater stocking process of intermediate-raised fish is criticized for its low economic efficiency because it requires a lot of cost compared to the effect of increasing the growth rate.

In order to overcome this, a method of stocking in seawater at the fry stage and growing at a high speed is used, but research is limited due to the high mortality rate.

(0001) Republic of Korea Patent No. 10-1751180 (0002) Republic of Korea Patent Publication No. 10-2015-0055214

The present invention has been devised to solve the above problems, and an object of the present invention is to acclimatize the fry of trout in seawater to grow the trout at a faster rate than the conventional aquaculture method. to provide a way to reduce

Another object of the present invention is to provide a method for reducing the mortality rate in the process of acclimatization of trout by optimizing the acclimatization process of trout, which can minimize the mortality that may occur in the acclimatization process of fry size trout.

Another object of the present invention relates to a method for reducing the mortality rate of trout in the seawater treatment process of trout with improved meat quality and growth rate by generating neutralized trout during fertilization of trout.

The problems of the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to solve the above problems, the present invention comprises the steps of: growing the modified trout fry to 10 to 30 g of body weight; a first step of acclimatizing the grown fry at low salinity; 1st and 2nd salinity raising step of increasing salinity after the 1st acclimatization step is completed; a second step of maintaining salinity after the second step of increasing salinity is completed; and a third salinity raising step of increasing the salinity to the salinity of the seawater after the second acclimatization step.

In one embodiment, the method of reducing the mortality rate in the process of acclimatization of the trout includes a primary acclimatization step of acclimatizing the grown fry for 5 to 10 days at a salinity of 5 to 6 psu; a first salinity raising step of increasing to 20 psu at a rate of 1 to 1.5 psu/day after the first acclimatization step is completed; a second salinity raising step of increasing to 24-26 psu at a rate of 2 psu/day after the first salinity raising step is completed; a second acclimatization step of maintaining for 3 to 5 days after the second salinity raising step is completed; and a tertiary salinity increasing step of increasing to 33 to 36 psu at a rate of 0.5 to 1.5 psu/day after the second acclimatization step, wherein the trout fry are pre-female triploid trout from which fertility has been removed, and the pre-female 3 Diluting the diploid trout by adding 800 to 1200 parts by weight of physiological saline to 100 parts by weight of semen collected from adult male trout; inactivating the Y chromosome by irradiating ultraviolet light to the semen diluted with the physiological saline; fertilization by adding the inactivated semen to eggs collected from adult trout females; And after the modification is completed, it is left for 5 to 15 minutes and subjected to a temperature shock at a temperature of 25 to 30° C. for 10 to 30 minutes to suppress the emission of the second polar body, and in the step of inactivating The used ultraviolet rays are irradiated for 10-20 minutes at an intensity of 2000-2500 μW/cm 2 ; The trout culture is, aquaculture; a photobioreactor to which water drained from the aquaculture tank is supplied; And the water that has passed through the photobioreactor is supplied and filtered, and it is carried out in a culture system comprising a filtration tank for supplying the filtered water to the aquaculture tank, wherein the photobioreactor receives the water supplied from the aquaculture tank a distribution pipe supplying the bioreactor body; 1 to 30 photobioreactor bodies that contain microalgae and water therein, and perform a photobiological reaction using water supplied from the distribution pipe; a water supply unit for supplying water discharged from the photobioreactor body to a filtration tank; and a photobioreactor frame for fixing the photobioreactor at a predetermined position, wherein the photobioreactor body includes: a film-type body made of a polymer film to form a reaction space therein; Microalgae located in the reaction space and performing a photobiological reaction; fixing means for fixing the film-type body to the frame; an injection unit connected to the lower portion of the film-type body to inject water supplied from the distribution pipe into the reaction space; and a discharge unit connected to the upper portion of the film-type body and supplying water from the inside of the reaction space to the water supply unit, wherein the photobioreactor frame includes: an upper horizontal frame to which an upper end of the photobioreactor body is fixed; a lower horizontal frame to which the lower end of the photobioreactor body is fixed; a vertical frame connecting the upper horizontal frame and the lower horizontal frame so that the photobioreactor body can maintain a constant height; and a light supply frame formed between the vertical frames, connecting the upper horizontal frame and the lower horizontal frame, and having 2 to 100 LEDs arranged in the longitudinal direction, wherein the filtration tank includes an aerobic tank and an anaerobic tank, ; The exhalation tank is formed by a four-sided wall and a floor, and the upper end is at the same height as the upper end of the four-sided wall, and the front end is fixed in the middle of the inner surface of the front wall among the four walls, and extends from the rear end of the front part to the left at right angles The left end is fixed to the rear of the inner surface of the seat wall among the four walls so as to be isolated from the inner surface of the rear wall among the four walls, and is partitioned by a first diaphragm having a flow hole drilled at the lower end of the rear wall, a precipitation chamber for precipitating water that has passed through the reactor; The height of the upper end is lower than the first diaphragm, the left end is fixed to the rear end of the right side of the front part, and the right end is partitioned to be positioned at the rear of the settling chamber by a second diaphragm fixed to the rear of the right side wall among the four walls. a first filtration chamber for primary filtration of water that has passed through the settling chamber; The height of the upper end is lower than the first diaphragm and higher than the second diaphragm, and between the second diaphragm and the right side of the front wall among the four-sided walls, the left end is closely fixed to the right side of the front side of the first diaphragm, and the right end is fixed to the right side wall of the four-sided wall It is fixed and the lower end is installed to be separated from the floor and is partitioned in front of the right side of the first filter chamber by a third diaphragm that allows a distribution part to be formed between the lower end and the floor to secondary filter the water that has passed through the first filter chamber second filtration chamber; a third filter chamber that is partitioned in front of the second filter chamber by the third partition plate and tertiarily filters water passing through the second filter chamber; a supply pipe having an upper end fixed to a hole drilled on the upper right side of the front wall among the four side walls to supply water from the third filtration chamber to the anaerobic tank; a mineral material, a sponge filter, and a non-woven fabric filter stacked sequentially from the bottom in the precipitation chamber; and a mineral material laid on the floor of the first to third filter chambers; The anaerobic tank, an anaerobic tank body formed of four walls, an upper portion and a bottom; a rotating shaft coupled to the upper portion of the main body to rotate the impeller inside the main body at a constant speed; an impeller connected to one end of the rotation shaft to mix water inside the body; a motor connected to the other end of the rotating shaft to rotate the rotating shaft; a supply unit connected to the lower end of the left side wall among the four walls of the anaerobic tank and injecting water supplied from the third filtration chamber into the anaerobic tank; a discharge unit connected to the upper right side of the four walls of the anaerobic tank to discharge water from which the aerobic reaction is completed and supply it to the aquaculture tank; and an organic component supply unit for supplying an organic component to the anaerobic tank.

According to an embodiment of the present invention, the mortality rate is reduced in the process of acclimatization of trout, which can grow trout at a faster rate compared to the conventional trout culture method by growing the fry of trout by acclimatization to the same concentration of salinity as seawater. method can be provided.

In addition, the present invention can generate polyploid trout by collecting the semen of trout and then treating it according to a specific treatment method, and thus a method of reducing the mortality rate in the process of trout in seawater in which the growth of the trout is maximized. can provide

In addition, the present invention circulates the water of the aquaculture tank when trout is cultured. Compared to the existing trout culture method, the cost incurred when replacing water can be minimized, and the trout excrement can be treated, which is more economical than the conventional method. It may provide a method for reducing mortality in the brine acclimatization process of phosphorus trout.

The effect according to the present invention is not limited by the contents exemplified above, and more various effects are included in the present invention.

1 is a comparison of the red blood cell size of (a) and (b) normal diploid trout (c) and (d) as the result of observation of red blood cells according to an embodiment of the present invention.
2 is a photograph showing a gonad biopsy according to an embodiment of the present invention. (a) is a normal trout ovary, (b) is a normal trout testis, and (c) is a triploid trout ovary.
3 is a graph showing the mortality rate according to the date according to an embodiment of the present invention.
Figure 4 shows the overall configuration of the aquaculture system according to an embodiment of the present invention.
5 shows the structure of a photobioreactor according to an embodiment of the present invention.
6 shows the structure of an aerobic tank according to an embodiment of the present invention.
Figure 7 shows the structure of the anaerobic tank h according to an embodiment of the present invention.

Hereinafter, various embodiments will be described in more detail with reference to the accompanying drawings. The embodiments described herein may be variously modified. Certain embodiments may be depicted in the drawings and described in detail in the detailed description. However, the specific embodiments disclosed in the accompanying drawings are only for easy understanding of various embodiments. Therefore, the technical spirit is not limited by the specific embodiments disclosed in the accompanying drawings, and it should be understood to include all equivalents or substitutes included in the spirit and scope of the invention.

Terms including an ordinal number, such as first, second, etc., may be used to describe various elements, but these elements are not limited by the above-described terms. The above terminology is used only for the purpose of distinguishing one component from another component.

In this specification, terms such as "comprises" or "have" are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof. When a component is referred to as being “connected” or “connected” to another component, it is understood that the other component may be directly connected or connected to the other component, but other components may exist in between. it should be On the other hand, when it is said that a certain element is "directly connected" or "directly connected" to another element, it should be understood that no other element is present in the middle.

Meanwhile, as used herein, a “module” or “unit” for a component performs at least one function or operation. And “module” or “unit” may perform a function or operation by hardware, software, or a combination of hardware and software. In addition, a plurality of “modules” or a plurality of “units” other than a “module” or “unit” that must be performed in specific hardware or are executed in at least one processor may be integrated into at least one module. The singular expression includes the plural expression unless the context clearly dictates otherwise.

In addition, in describing the present invention, when it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be abbreviated or omitted.

The present invention comprises the steps of growing the fertilized trout fry up to 10-30 g of fish body weight; a first step of acclimatizing the grown fry at low salinity; 1st and 2nd salinity raising step of increasing salinity after the 1st acclimatization step is completed; a second step of maintaining salinity after the second step of increasing salinity is completed; and a third salinity raising step of increasing the salinity to the salinity of the seawater after the second acclimatization step.

The existing trout farming method mainly consists of cultivating trout in fresh water. It is widely used in land-based aquaculture facilities because it does not require brine acclimatization of trout and does not require the cost of preparing brine or transporting the trout to the sea. However, in the case of trout, when grown by acclimatization to brine, the growth rate increases, which is known to be more economical than the brine production cost and transport cost.

However, acclimatization with brine in the fry stage has a high mortality rate, so most aquaculture methods use acclimatization of trout weighing less than 100 g. To acclimatize in such an intermediate breeding state of about 100 g, not only the amount of salt water required is large, but also the cost of moving to the beach to use sea water is required. In addition, in the case of acclimatization at the fry stage, the period it takes to reach the size of 1.5 kg sold in the market can be shortened to about 14 months, which is far more economical than the 20 months of acclimatization of intermediate breeding fish.

However, when acclimatization of fry with a body weight of less than 50 g in brine, the mortality rate may be high.

To this end, the trout breeding method includes a first breeding step of acclimatizing the grown fry at a salinity of 5-6 psu for 5-10 days; a first salinity raising step of increasing to 20 psu at a rate of 1 to 1.5 psu/day after the first acclimatization step is completed; a second salinity raising step of increasing to 24-26 psu at a rate of 2 psu/day after the first salinity raising step is completed; a second acclimatization step of maintaining for 3 to 5 days after the second salinity raising step is completed; and a third salinity raising step of increasing to 33 to 36 psu at a rate of 0.5 to 1.5 psu/day after the second acclimatization step.

The first acclimatization step is a step of stocking the fry grown to 10 to 30 g of the fish body weight in low-concentration brine and acclimatizing the fry. At this time, if the fry of the trout have a fish weight of less than 10 g, the mortality rate may increase in the acclimatization stage, and if it exceeds 30 g, the amount of brine required increases and the cost used for movement may increase, which is uneconomical.

In the first acclimatization step, it is preferable to acclimatize the fry for 5 to 10 days at a salinity of 5 to 6 psu. At this time, if the salinity is less than 5 psu, the salinity change may be abrupt in the acclimatization step to be described later, and the mortality rate may increase. In addition, if the first acclimatization stage is less than 5 days, the salinity adaptation of the fry is incomplete, and the mortality rate may increase in the acclimatization stage to be described later.

After the first acclimatization step is completed, the first and second salinity raising steps of increasing the salinity to intermediate salinity may be performed. This salinity raising step is a step of gradually increasing the salinity to a medium-strong salinity stage, which is the maximum salinity at which the fry that have passed through the first acclimatization stage do not die. It may consist of a secondary salinity raising step that raises the

The first salinity raising step is a step to increase the salinity adaptability of the fry by increasing the salinity at a low rate, and is a step of adjusting the salinity control mechanism in the fry by raising the flue at a low rate. In this case, in the first salinity raising step, it is preferable to increase the salinity to 20 psu at a rate of 1 to 1.5 psu/day. If the rate of salinity increase is less than 1 psu / day, it is uneconomical because it requires a lot of time to increase the salinity. .

Through the first salinity raising step, the fry adapt to the increasing salinity, so it is possible to quickly increase the salinity to reach the intermediate salinity. This is called the secondary salinity raising step, and the salinity can be increased to 24 to 26 psu at a rate of 2 psu/day. At this time, if the salinity is raised to less than 24 psu, the mortality rate of fry is reduced in the second acclimatization stage, but the mortality rate may increase in the tertiary salinity raising stage. can rise

After the second salinity raising step is completed, a second acclimatization step may be performed for 3 to 5 days. The 24-26 psu salinity section, that is, the intermediate salinity step, is a step with the highest mortality rate of fry, and it is preferable to lower the mortality rate of fry by acclimatizing in this section. At this time, the fry can maximize the body mechanism for discharging salt in the intermediate salinity stage, and thus can be easily acclimatized even at the final salinity to be described later, that is, the same salinity as seawater. At this time, if the secondary acclimatization stage is less than 3 days, the mortality rate may increase in the tertiary acclimatization stage.

When the secondary acclimatization is completed, the acclimatized fry may go through a tertiary salinity raising step to instantaneously reach the salinity of seawater. In this case, the third salinity raising step is preferably increased to 33 to 36 psu at a rate of 0.5 to 1.5 psu/day. In this step, since it is acclimatized at high salinity, it is preferable to increase the salinity at a low rate compared to the first and second salinity raising steps. When the rate of the third salinity raising step is less than 0.5 psu/day, the real time required to increase the salinity is long, which is uneconomical, and when the rate is higher than 1.5 psu/day, the mortality rate may increase. Through this, the salinity can be increased to 33-36 psu, which is the salinity of seawater, which can be appropriately adjusted according to weather conditions and the state of the fry.

In the case of trout fry used in the present invention, it is preferable to use all female triploid trout from which fertility has been removed for rapid growth. In general, when a trout grows to a certain size or more, testes or egg nests (ovaries or eggs) are grown for reproduction. This is nature's providence for generation reproduction, but in terms of aquaculture, it is inefficient because the supplied energy is not used for growth. In particular, in the case of trout with limited intake of testes and ovaries, the growth of the testes and ovaries may act as an obstacle to body growth. Therefore, in the case of the fry, it is possible to minimize the growth of testes or ovaries by using pre-female triploid trout from which fertility has been removed, so that they can be rapidly grown to a size that can be shipped to the market.

To this end, the pre-female triploid trout is diluted by adding 800 to 1200 parts by weight of physiological saline to 100 parts by weight of semen collected from adult male trout; inactivating the Y chromosome by irradiating ultraviolet light to the semen diluted with the physiological saline; fertilization by adding the inactivated semen to eggs collected from adult trout females; And it can be modified in a method comprising the step of suppressing the emission of the second polar body by allowing the correction to be left for 5 to 15 minutes and applying a temperature shock for 10 to 30 minutes at a temperature of 25 to 30 ℃ after the modification is completed.

Since the concentration of semen collected from the adult male is high, UV rays may not be transmitted to all sperm during UV treatment, which will be described later. Therefore, it is preferable to dilute it with physiological saline so that the ultraviolet rays can be uniformly transmitted to the sperm. At this time, the physiological saline is preferably added in an amount of 800 to 1200 parts by weight based on 100 parts by weight of the collected semen. When the physiological saline solution is contained in an amount of less than 800 parts by weight, untreated sperm may be generated in excess of the standard value in the UV irradiation process to be described later.

The diluted semen can be irradiated with ultraviolet light to inactivate the Y chromosome. At this time, it is preferable to irradiate the ultraviolet rays with an intensity of 2000-2500 μW/cm 2 for 10-20 minutes. When the ultraviolet ray is irradiated with an intensity or time less than the above range, the inactivation rate of the Y chromosome is lowered, making it difficult to obtain a desired level of pre-female triploid trout. Sperm can be inactivated, which can reduce fertility.

After the Y chromosome is inactivated by ultraviolet light as described above, the semen may be mixed with an egg (egg) collected from an adult female trout and fertilized. This process can be performed through the dry fertilization method used in the past. In this process, the inactivated Y chromosome sperm may be fertilized with the egg, and a normal diploid fertilized egg and a triploid fertilized egg may be generated. It is preferable to leave it for 5 to 15 minutes for smooth fertilization of the sperm and egg.

After the correction is completed as described above, it is preferable to suppress the emission of the second polar body by applying a temperature shock at a temperature of 25 to 30° C. for 10 to 30 minutes. In this process, the sperm and the fertilized egg having the inactivated Y chromosome may undergo chromosomal doubling, which may increase the hatching rate of the fertilized egg having the inactivated chromosome. When the second polar body release suppression process is performed below the temperature and time range, the second polar body release is not suppressed, so the hatchability of fertilized eggs having a triploid may fall, and if it exceeds the above range, the overall The hatchability may be reduced.

In the case of trout fry having diploid and triploid fertilized eggs as described above, the average hatching rate may be 80% or more. Since the conventional trout fertilized egg hatching rate is also 80% or more, it can have a similar hatching rate even after the above process. However, if the heat treatment process is not performed, the incubation rate may be greatly reduced because the fertilized eggs in which the Y chromosome is inactivated die before hatching.

In addition, in the case of the trout culture method, it corresponds to a process of changing the salinity by continuously supplying brine. In general, in the case of brine used in the acclimatization process as described above, seawater can be used, but since the acclimatization process is inevitably performed in an inland aquaculture tank, a large cost may occur when the seawater is continuously used. Therefore, it is possible to reduce the cost used in the trout culture process by recirculating the purifying the seawater.

To this end, the trout culture is a farm; a photobioreactor to which water drained from the aquaculture tank is supplied; And the water that has passed through the photobioreactor is supplied and filtered, and it may be carried out in a culture system comprising a filtration tank for supplying the filtered water to the aquaculture tank.

The photobioreactor is a part that supplies oxygen to the water using nutrients of the water discharged from the aquaculture tank 100, that is, excrement of trout as nutrients. It can be performed through the photobioreactor 200 using microalgae. .

To this end, the photobioreactor includes: a distribution pipe for supplying water supplied from the aquaculture tank to each photobioreactor body; 1 to 30 photobioreactor bodies that contain microalgae and water therein, and perform a photobiological reaction using water supplied from the distribution pipe; a water supply unit for supplying water discharged from the photobioreactor body to a filtration tank; and a photobioreactor frame for fixing the photobioreactor at a predetermined position.

A general photobioreactor does not have a large processing capacity and takes a lot of processing time. Therefore, when using the photobioreactor in direct connection, it can pass through the photobioreactor before supplying desired oxygen. In addition, in the case of using a large-sized photobioreactor to overcome this, it may be difficult to handle, and it may be difficult to create and manage the environment inside the reactor. Therefore, in order to overcome this problem, desired oxygen supply can be performed by installing a plurality of photobioreactors having a certain size in parallel, and the management can also be facilitated.

The distribution pipe 210 distributes and supplies the water supplied from the aquaculture tank to each photobioreactor 220 , and one end is coupled to the aquaculture tank, and the other end is to be coupled to the photobioreactor body 220 . can In this case, the distribution pipe 210 may connect the distribution pipe connected to the aquaculture tank to have a plurality of branches, and through this, the water passing through the culture tank may be supplied to the plurality of photobioreactor bodies 220 . In addition, a valve or a fitting may be provided at the other end of the distribution pipe for separation of the photobioreactor. That is, when separating or connecting only some of the photobioreactors for management of the photobioreactor, water can be supplied to the desired photobioreactor by blocking the flow of some distribution pipes using the valve or fitting.

The photobioreactor body 220 serves to remove nutrients and supply oxygen to the water by culturing the microalgae therein using the water supplied from the distribution pipe 210 . To this end, a film-type body made of a polymer film to form a reaction space therein; Microalgae located in the reaction space and performing a photobiological reaction; fixing means for fixing the film-type body to the frame; an injection unit connected to the lower portion of the film-type body to inject water supplied from the distribution pipe into the reaction space; and a discharge part connected to the upper portion of the film-type body and supplying water in the reaction space to the filtration tank.

The film-type body 221 is a part constituting the outer surface of the photobioreactor, and as it is manufactured using a film, it is possible to secure a certain space (reaction space) therein. This film-type body 221 can simply block the upper and lower ends of the tubular film to produce a cylindrical reactor, but a photobioreactor can be manufactured by bonding two film edges and then supplying water and microalgae between the films. . In the case of a photobioreactor manufactured by bonding such films, the thickness of the reactor can be made thin, so it can have the advantage of superior light efficiency compared to a cylindrical reactor. In addition, the film-type reactor is light in weight compared to the conventional reactor using a plate made of polymer or glass, and the reactor itself has ductility and is strong against impact, so it is easy to handle. In addition, the film-type body is preferably made of a transparent material for the growth and reaction of the microalgae. That is, since the film-type reactor is transparently manufactured and has a thin thickness, the light transmission efficiency is excellent, and a large amount of oxygen can be supplied to the water. In addition, when it is necessary to block light of some wavelengths for selective culturing of microalgae, it is possible to block light of a specific wavelength or transmit only light of a specific wavelength by making the film to have a specific color.

The reaction space may contain microalgae. The microalgae are microorganisms that generate oxygen by using carbon dioxide present in water. In the case of the existing ornamental fish culture system, since oxygen is supplied using aeration or an oxygen pump, the gas efficiency is low, but in the case of the present invention, the photobioreactor Since oxygen is supplied using microalgae in the interior, carbon dioxide reduction and oxygen increase can be simultaneously performed. In this case, the microalgae can be used without limitation as long as they can provide the oxygen, and it is also possible to collect the microalgae and process it into aquaculture feed.

The fixing means 222 is a means for fixing the film-type body 221 in a predetermined space, and at the same time vertically fixing the film-type body 222, a plurality of film-type bodies can be arranged and fixed. Through this, a plurality of film-type bodies may be arranged in the photobioreactor, and it is preferable to supply oxygen to a desired amount of water using a plurality of film-type bodies.

The film-type body 221 may include a water inlet 224 for injecting water and a water outlet 223 for discharging the reaction-completed water. In the case of the water inlet 224, it may be located at the bottom of the film-type body for smooth injection, and in the case of the water outlet 223, it is installed in the upper part of the body, and water that has stayed in the reactor for a certain time It is preferable to discharge through the outlet. In addition, in the case of the water outlet 223, a filter may be installed so that the microalgae inside are not mixed and discharged.

The photobioreactor frame may include an upper horizontal frame to which an upper end of the photobioreactor body is fixed; a lower horizontal frame to which the lower end of the photobioreactor body is fixed; a vertical frame connecting the upper horizontal frame and the lower horizontal frame so that the photobioreactor body can maintain a constant height; and a light supply frame formed between the vertical frames, connecting the upper horizontal frame and the lower horizontal frame, and having 2 to 100 LEDs arranged in the longitudinal direction.

The photobioreactor frame may have the photobioreactor body installed at regular intervals, and upper and lower ends may be fixed to maintain a constant shape. To this end, the photobioreactor body may have an upper end connected to the upper horizontal frame 241 , and a lower end connected to the lower horizontal frame 242 .

In addition, a light supply frame may be included between the vertical frames 243 . The light supply frame is a means for supplying light necessary for the microalgae to perform photosynthesis, and is formed in the same vertical direction as the vertical frame 243 so that a plurality of LEDs may be attached to the surface. Through this, the light supply frame can supply a large amount of light to the photobioreactor.

In particular, in the case of the recently used LED, it is preferable to use an LED to supply light to the photobioreactor as described above because it can emit light of a desired wavelength by combining it with a fluorescent material, and has a small size and high power efficiency. .

In addition, 2 to 100 LEDs per one light supply frame, preferably 5 to 20 LEDs, may be arranged in the longitudinal direction. In this case, when only one LED is installed, it may be difficult to supply light smoothly, and when more than 100 LEDs are arranged, power consumption increases, which is inefficient.

The water passing through the photobioreactor may be filtered and purified through a filtration tank. To this end, the filtration tank may include an aerobic tank 300 and an anaerobic tank 400 .

The aerobic tank 300 is installed to perform an aerobic reaction, and the upper end is at the same height as the upper end of the four-sided wall in the interior formed by the four walls and the floor, and the front end is fixed in the middle of the inner surface of the front wall among the four walls It extends to the left at right angles from the rear end of the front side and the front side, and the left end is fixed to the rear side of the inner surface of the seat wall among the four side walls so as to be isolated from the inner surface of the rear wall among the four side walls. a precipitation chamber that is partitioned by a first diaphragm to first precipitate water that has passed through the photobioreactor; The height of the upper end is lower than the first diaphragm, the left end is fixed to the rear end of the right side of the front part, and the right end is partitioned to be positioned at the rear of the settling chamber by a second diaphragm fixed to the rear of the right side wall among the four walls. a first filtration chamber for primary filtration of water that has passed through the settling chamber; The height of the upper end is lower than the first diaphragm and higher than the second diaphragm, and between the second diaphragm and the right side of the front wall among the four-sided walls, the left end is closely fixed to the right side of the front side of the first diaphragm, and the right end is fixed to the right side wall of the four-sided wall It is fixed and the lower end is installed to be separated from the floor and is partitioned in front of the right side of the first filter chamber by a third diaphragm that allows a distribution part to be formed between the lower end and the floor to secondary filter the water that has passed through the first filter chamber second filtration chamber; a third filter chamber that is partitioned in front of the second filter chamber by the third partition plate and tertiarily filters water passing through the second filter chamber; a supply pipe having an upper end fixed to a hole drilled on the upper right side of the front wall among the four side walls to supply water from the third filtration chamber to the anaerobic tank; a mineral material, a sponge filter, and a non-woven fabric filter stacked sequentially from the bottom in the precipitation chamber; And it may be provided with a mineral material spread on the floor of the first to third filter chambers.

The settling chamber 310, the upper end is at the same height as the upper end of the aerobic tank 300, the front end is fixed in close contact with the inner surface of the middle part of the front wall of the aerobic tank, and the front end is extended at right angles from the rear end of the front end to the left. A "L" shape provided with a rear portion having the left end tightly fixed to the rear inner surface of the seat wall so as to be separated from the inner surface of the rear wall of the aerobic tank at a predetermined distance, and a distribution port drilled through the first filter chamber at the lower end of the rear portion formed by the first diaphragm of

In addition, in the settling chamber 310, a mineral material, a sponge filter, and a non-woven fabric filter are sequentially stacked from the bottom, and the water supplied from the photobioreactor flows in and passes through the non-woven fabric filter and the sponge filter in order to physically filter out impurities. Of course, biological filtration is simultaneously performed by the aerobic microorganisms located inside the filter and the filtration tank, and then minerals are supplied through the minerals.

The first filtration chamber 320, the height of the upper end is lower than the first diaphragm, the left end is closely fixed to the right rear end of the front side of the settling chamber, and the right end is tightly fixed to the rear side of the right side wall of the aerobic tank to the rear wall of the aerobic tank It is formed to be positioned at the rear of the settling chamber and the second filtration chamber 330 by the second diaphragm installed in parallel with the diaphragm, and may be provided with a mineral material laid on the floor to a predetermined thickness.

This first filtration chamber 320, in the settling chamber 310, through the flow port formed at the lower end of the first diaphragm, the mineral is supplied in the process of filling up the water flowing through the mineral from the bottom, and at the same time impurities are removed. If there is, it can be adsorbed and removed.

And, when the water level rises, water may be introduced into the upper part of the second filtration chamber through the upper end of the second diaphragm, which is lower in height than the upper end of the first diaphragm.

The second filtration chamber 330 has an upper end lower than the first diaphragm and higher than the second diaphragm, and the left end between the second diaphragm and the right side of the front wall of the aerobic tank is tightly fixed to the right side of the front side of the first diaphragm and the right end is closely fixed to the right side wall of the aerobic tank, and the lower end is positioned in front of the right side of the first filtration chamber 320 by a third diaphragm installed to be separated from the floor of the aerobic tank by a predetermined interval. It may be provided with a distribution part formed in communication with the lower part of the third filtration chamber 340 by a predetermined interval between the lower part and the bottom of the aerobic tank and the mineral material to be spread.

In this second filtration chamber 330, the water filtered and supplied with minerals in the first filtration chamber 320 flows into the upper part beyond the upper end of the second diaphragm, flows downward, and passes through the mineral matter laid on the floor. If there is an impurity, it is adsorbed and removed at the same time as further supply, and the water passing through the mineral may be introduced into the lower part of the third filtration chamber through the distribution unit.

The third filtration chamber 340 is formed to be positioned in front of the second filtration chamber 330 by a third diaphragm, and is coupled to the mineral material laid on the floor and a hole drilled in the upper right side of the front wall of the aerobic tank. A supply pipe for supplying water from the third filtration chamber to the anaerobic tank 400 to be described later may be provided.

This third filtration chamber 340, the water filtered and supplied with minerals in the second filtration chamber 330 flows into the lower part through the distribution unit and passes through the minerals laid on the floor as it fills up, so that more minerals are supplied. At the same time, if there is an impurity, it is adsorbed and removed, and when the water level reaches the upper end of the supply pipe 350, it flows down and can be supplied to the anaerobic tank 400 to be described later.

The anaerobic tank 400 is a place where the water filtered and aerobic treated in the aerobic tank 300 is treated using anaerobic bacteria. This anaerobic reaction can be performed to remove phosphorus and nitrogen components.

To this end, the anaerobic tank includes an anaerobic tank body formed of four walls, an upper part and a bottom; a rotating shaft coupled to the upper portion of the main body to rotate the impeller inside the main body at a constant speed; an impeller connected to one end of the rotation shaft to mix water inside the body; a motor connected to the other end of the rotating shaft to rotate the rotating shaft; a supply unit connected to the lower end of the left side wall among the four walls of the anaerobic tank and injecting water supplied from the third filtration chamber into the anaerobic tank; a discharge unit connected to the upper right side of the four walls of the anaerobic tank to discharge the water from which the aerobic reaction is completed and supply it to the photobioreactor; and an organic component supply unit for supplying an organic component to the anaerobic tank.

The main body 410 is a part that forms the reaction space of the anaerobic tank, and since it is necessary not to supply oxygen in contact with air, it may be manufactured in a closed type unlike the aerobic tank. Therefore, inside the anaerobic microorganisms can easily perform the reaction, through which it is possible to remove the phosphorus and nitrogen powder contained in the water that has passed through the aerobic tank.

The rotating shaft 420 is installed through the upper part of the anaerobic tank, and the impeller 430 is installed on one side and the motor is installed on the other side, that is, outside of the anaerobic tank, It can serve to rotate the impeller 430. . The rotation shaft 420 passes through the upper part of the anaerobic tank, but may be connected using a sealing means so that air does not flow to the penetrated part.

The impeller 430 is connected to one side of the rotation shaft 420 to continuously mix the water inside the anaerobic tank as a means for mixing the water inside the anaerobic tank, thereby helping the activity of anaerobic bacteria. At this time, the impeller 430 may simply use a propeller of 2 to 6 blades, but in this case, high-speed rotation is required to mix the entire anaerobic tank, and this high-speed rotation may adversely affect anaerobic bacteria, so many An impeller comprising four blades may be used.

The impeller 430 has a central portion connected to one end of the rotation shaft 420, a main wing extending along the inner surface of the anaerobic tank, and an auxiliary wing connecting the middle of the main wing and the middle of the rotating shaft 420 can be Through this, even when the impeller rotates at a low speed, it is possible to mix up to the corner of the anaerobic tank, and through this, the local adverse reaction is minimized and thus a desired removal efficiency can be obtained.

In addition, the main wing of the impeller 430 may have a “U”-shaped main wing manufactured as a straight string or flat plate as described above, but the main wing may be installed to rotate in a clockwise or counterclockwise direction. . In this case, the upper and lower flow of the water may be stronger by the rotation of the main blade, which means that the efficiency by the impeller may be increased.

In addition, the anaerobic tank may be manufactured to have 2 to 10 layers (see FIG. 7 ). In the case of the anaerobic reaction, it is preferable to remove phosphorus and nitrogen by performing an anaerobic reaction for a predetermined time. In order to secure such a residence time, the anaerobic tank is manufactured in 2 to 10 layers, each impeller is installed in each layer, and a constant vent is formed between each layer to maintain a constant residence time of the water flowing into the anaerobic tank. can In this case, the impeller may be connected to each motor, but a plurality of impellers may be connected in series to one motor and a rotating shaft.

The supply part 440 and the discharge part 450 are parts for injecting the water that has passed through the aerobic tank into the anaerobic tank or for discharging the water after the anaerobic reaction has been completed. can be located in Through this, the water that has passed through the aerobic tank is supplied to the lower part of the anaerobic tank, stays in the anaerobic tank for a certain period of time, performs an aerobic reaction, and can be discharged through the outlet.

In addition, since it is preferable to separate and discharge the anaerobic microorganisms and water in the case of the discharge unit, a filter for separating them may be installed in the discharge unit. In the case of this filter, a centrifugal separator or a centrifugal force separator such as a cyclone may be used, but a filter having an appropriate pore size may be installed to separate the anaerobic microorganisms and water.

In addition, the anaerobic reaction can be carried out only when an organic component is present. A general anaerobic reaction may be performed using an organic material contained in water, but if there are many nitrogen or phosphorus components, the anaerobic reaction may not be completed with only the organic material contained in the water. Therefore, in this case, it is preferable to supplement the insufficient organic component by adding an organic component supply unit to the anaerobic tank in order to supplement the organic material. Methanol is most suitable as the organic component, but in the case of methanol, if it remains in water, it may have a bad effect on the trout, and it may be difficult to handle because it is harmful to the human body. Therefore, in the present invention, it is preferable to supply sugar by mixing it with water.

In addition, since the anaerobic tank may generate nitrogen gas as a result of the anaerobic reaction, a one-way valve for discharging it may be installed in the upper part of the anaerobic tank. In the case of the one-way valve, when the discharged nitrogen reaches a certain pressure, the valve is opened and serves to discharge the nitrogen gas inside. Using a one-way valve so that external oxygen is not supplied to the inside of the anaerobic tank desirable.

As described above, the water that has passed through the anaerobic tank can be recycled to the fish tank, and thus the trout can be cultured while minimizing the additional replenishment of seawater.

Hereinafter, preferred embodiments of the present invention will be described so that those of ordinary skill in the art can easily implement them with reference to the accompanying drawings. In addition, in the description of the present invention, if it is determined that a detailed description of a related known function or a known configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted. In addition, certain features presented in the drawings are enlarged, reduced, or simplified for ease of description, and the drawings and components thereof are not necessarily drawn to scale. However, those skilled in the art will readily appreciate these details.

Example 1

Male trout (n=5, total length: 63.4±2.3cm; weight: 3,415±576.8g) and females (n=17, total length: 58.6± 4.2 cm; body weight: 3,519±835.7 g) was used in the experiment.

Instead of inducing physiological sex change using sex hormones, ultraviolet irradiation was used for environmentally friendly genetic sex change. The sperm collected by pressing the male's abdomen were diluted 10-fold with physiological saline and irradiated with UV light on ice (2,070-2,200 μW/cm2 x 15 minutes) to inactivate the Y chromosome. Chromosome ploidy was induced by suppressing the release of the second polar body by applying a temperature shock (28° C., 20 minutes) to the fertilized egg 10 minutes after fertilization with inactivated semen by the dry method.

The hatchability and germination rate of the fertilized eggs treated as described above were found to be 85% on average.

In general, when the sperm inactivated by UV light are fertilized with normal eggs, all of them die. Therefore, the fact that a relatively high hatching rate and hatching rate was achieved by giving a temperature shock after UV treatment as described above, even in trout. This suggests that the production of seedlings is possible.

In addition, the mortality rate by the early stage of the larvae that eats larvae is around 25%, so it is judged that mass production of fertile female seedlings using UV treatment and high temperature treatment methods is possible.

Experimental Example 1

To check the chromosomal ploidy of the produced seedlings, the size of red blood cells collected from the head pituitary artery of a 6-month-old larva at random was measured (n=20), and the Nucleolus organizer regions (NOR) test method (Kim et al., 2017) was also used. to confirm the polyploid.

The NOR test method used at this time is specifically as follows.

1. Fixation of the sample

--> Cut the caudal fin of about 1mm into small pieces in 1.5ml EP tube and fix the tissue in Carnoy's fixative.

2. Place the fixed sample fragment on the slide glass and cut it into small pieces (Mince)

--> Using a 1ml syringe, mince the tissue as finely as possible under a stereomicroscope.

3. Drop 100ul of 50% Acetic acid and dry at room temperature.

4. After drying, Silver Sol. A (1 drop), Silver Sol. Add B (2drop) and mix (minimize exposure to light).

5. Put it on a hot plate (60℃) and wait until it changes to golden-brown color.

--> Put enough silver dye on the chopped tissue (~200ul) and dye it for 6~7 minutes.

6. After staining, wash with D.W and dry at room temperature

--> Wash 3 times with DW carefully so that the tissue does not fall off, and after drying at room temperature, inspect the NOR under a microscope. (If two nuclei are visible in the cell, it is determined as a diploid; if three nuclei are visible, it is determined as a triploid; if four nuclei are visible, it is determined as a tetraploid.)

In order to determine the sex of the fertilized egg of Example 1, the abdomen was incised from the larvae of about 8 months of age, the gonads were removed (n=21), and the sex was determined under a microscope by pressing on a slide glass. The size of red blood cells was about 1.5 times larger than that of normal diploids in 80% or more individuals (see FIG. 1), and in the gonad biopsy, 80% were female and 10% were hepatic, and the ratio of males was It was found to be very low at 10% (see FIG. 2).

Example 2

The fry were cultured using the fertilized eggs fertilized as triploid as described above, and then supplied to the aquaculture system in which the aquaculture tank, photobioreactor, aerobic tank and anaerobic tank shown in FIG.

At this time, for comparison with the triploid female of Example 1, the following three types of fry and intermediate fish were used.

1. A diploid female of 12~15cm in total length (19±2.2g, n=1,280)

2. A total length of 11~16cm, pre-female triploid (18±6.4g, n=1,530 rice)

3. Medium-grown fish with a total length of around 23-28cm (135±27g, n=1120 fish)

Experimental Example 2

The three types of trout prepared in Example 2 were subjected to brine inoculation in the following manner.

D1~D7 : Maintained for 1 week at 5~6psu salinity.

D8~D19: Increase salinity by 1~1.5psu per day (salinity is adjusted by introducing seawater little by little into the round tank)

Raise it to 20 psu. (Record the body weight, body length and pesa rate)

D20~D22: After increasing the salinity to 2psu by increasing 2psu per day, it is allowed to stand at 24~25psu for 3-4 days.

D23~D34: From 24~25psu to 34psu, increase by 1psu per day to complete seawater salinity to complete acclimatization.

The mortality rate according to each date is shown in FIG. 3 . As shown in FIG. 3 , the total mortality rate of pre-female diploid fry was 15.43%, 5.77% of pre-female diploid fry, and 3.35% of normal brood fish during the period of broodstock. appeared somewhat high. However, compared to the fact that the mortality rate of up to 70-90% of salmonids weighing between 50 and 75 g in seawater is known so far, this is a fairly good result. There was no significant difference, and it was confirmed that the seawater level of the fry of 15 to 20 g was possible.

Experimental Example 3

The rainbow trout (Oncorhynchus mykiss) is a tropical fish that must face a variety of environmental challenges throughout its life and constant changes in salinity and temperature during the transition from freshwater to saltwater. (15°C vs 5°C) interaction with water temperature for osmotic pressure control during salt acclimatization was tested using breeding fish, and the results were applied to the freshwater fish of fry.

After salinity and temperature treatment, fish transferred to seawater at 15°C were able to adapt to seawater within 2 to 4 days, but fish transferred to seawater at 5°C did not successfully adapt to salinity within 4 days, resulting in approximately 18% mortality. did. The group exposed to 5°C seawater showed significantly higher plasma ions and osmolality than the group exposed to 15°C. The level of gill Na+/K+ activity did not increase in the 5°C group, and the group acclimatized to 15°C showed a characteristic increase in gill Na+/K+ activity. The failure to upregulate gill Na+/K+ in fish exposed to 5 °C seawater suggests that this is a limiting mechanism for successful seawater adaptation. Therefore, it was confirmed that the water temperature between 12~15°C, rather than the low temperature of 5~8°C, helps the rainbow trout to acclimatize to normal salinity, thereby lowering the mortality rate.

In the above, preferred embodiments of the present invention have been illustrated and described, but the present invention is not limited to the specific embodiments described above, and it is common in the technical field to which the present invention pertains without departing from the gist of the present invention as claimed in the claims. Various modifications are possible by those having the knowledge of, of course, these modifications should not be individually understood from the technical spirit or prospect of the present invention.

100: aquaculture
200: photobioreactor
210: water supply unit
220: reactor body
221: film-type body
222: fixing means
223: discharge part
224: injection part
230: water outlet
241: upper horizontal frame
242: lower horizontal frame
243: vertical frame
300: aerobic
310: settling chamber
320: first filter room
330: second filter room
340: 3rd filter room
350: supply pipe
400: anaerobic tank
410: body
420: axis of rotation
430: impeller
440: supply
450: discharge part

Claims (2)

Growing the fertilized trout fry to a fish weight of 10 to 30 g;
a first step of acclimatizing the grown fry at low salinity;
1st and 2nd salinity raising step of increasing salinity after the 1st acclimatization step is completed;
a second step of maintaining salinity after the second step of increasing salinity is completed; and a third salinity raising step of increasing the salinity to the salinity of seawater after the second acclimatization step;
In the method of reducing the mortality rate in the seawater level process of trout comprising a,
A method to reduce the mortality rate in the process of seawater purification of trout is,
a first acclimatization step of acclimatizing the grown fry for 5 to 10 days at a salinity of 5 to 6 psu;
a first salinity raising step of increasing to 20 psu at a rate of 1 to 1.5 psu/day after the first acclimatization step is completed;
a second salinity raising step of increasing to 24 to 26 psu at a rate of 2 psu/day after the first salinity raising step is completed;
a second acclimatization step of maintaining for 3 to 5 days after the second salinity raising step is completed; and
a tertiary salinity raising step of increasing to 33-36 psu at a rate of 0.5-1.5 psu/day after the second acclimatization step;
includes,
The trout fry are pre-female triploid trout from which fertility has been removed,
The pre-female triploid trout,
diluting by adding 800 to 1200 parts by weight of physiological saline to 100 parts by weight of semen collected from adult male trout;
inactivating the Y chromosome by irradiating ultraviolet light to the semen diluted with the physiological saline;
fertilization by adding the inactivated semen to eggs collected from adult trout females; and
Suppressing the emission of the second polar body by leaving it to stand for 5 to 15 minutes after the modification is completed and applying a temperature shock for 10 to 30 minutes at a temperature of 25 to 30°C;
is modified in such a way as to include
The ultraviolet rays used in the inactivation step are irradiated with an intensity of 2000-2500 μW/cm 2 for 10-20 minutes;
trout farming,
aquaculture;
a photobioreactor to which water drained from the aquaculture tank is supplied; and
a filtration tank in which water that has passed through the photobioreactor is supplied and filtered, and the filtered water is supplied to the aquaculture tank;
is performed in a form system that includes
The photobioreactor,
a distribution pipe for supplying water supplied from the aquaculture tank to each photobioreactor body;
1 to 30 photobioreactor bodies that contain microalgae and water therein, and perform a photobiological reaction using water supplied from the distribution pipe;
a water supply unit for supplying water discharged from the photobioreactor body to a filtration tank; and
a photobioreactor frame for fixing the photobioreactor at a predetermined position;
includes,
The photobioreactor body,
a film-type body made of a polymer film to form a reaction space therein;
Microalgae located in the reaction space and performing a photobiological reaction;
fixing means for fixing the film-type body to the frame;
an injection unit connected to the lower portion of the film-type body to inject water supplied from the distribution pipe into the reaction space; and
a discharge unit connected to the upper portion of the film-type body and supplying water in the reaction space to the water supply unit;
includes,
The photobioreactor frame,
an upper horizontal frame to which an upper end of the photobioreactor body is fixed;
a lower horizontal frame to which the lower end of the photobioreactor body is fixed;
a vertical frame connecting the upper horizontal frame and the lower horizontal frame so that the photobioreactor body can maintain a constant height; and
a light supply frame formed between the vertical frames, connecting the upper horizontal frame and the lower horizontal frame, and having 2 to 100 LEDs arranged in the longitudinal direction;
includes,
the filtration tank includes an aerobic tank and an anaerobic tank;
The aerobic phase is
In the interior formed by the four-sided wall and the floor, the upper end is at the same height as the upper end of the four-sided wall, and the front end is fixed in the middle of the inner surface of the front wall among the four-sided walls, and extends at right angles to the left from the rear end of the front part. The left end is separated from the inner surface of the rear wall and is partitioned by a first diaphragm having a rear portion fixed to the inner surface rear side of the seat wall among the four side walls and a flow hole drilled at the lower end of the rear portion and passed through the photobioreactor. a precipitation chamber for precipitating water first;
The height of the upper end is lower than the first diaphragm, the left end is fixed to the rear end of the right side of the front part, and the right end is partitioned to be positioned at the rear of the settling chamber by a second diaphragm fixed to the rear of the right side wall among the four walls. a first filtration chamber for primary filtration of water that has passed through the settling chamber;
The height of the upper end is lower than the first diaphragm and higher than the second diaphragm, and between the second diaphragm and the right side of the front wall among the four-sided walls, the left end is tightly fixed to the right side of the front side of the first diaphragm, and the right end is fixed to the right side wall of the four-sided wall It is fixed and the lower end is installed to be separated from the floor, and is partitioned in front of the right side of the first filter chamber by a third diaphragm that allows a distribution part to be formed between the lower end and the floor to secondary filter the water that has passed through the first filter chamber second filtration chamber;
a third filter chamber that is partitioned in front of the second filter chamber by the third partition plate and tertiarily filters water passing through the second filter chamber;
a supply pipe having an upper end fixed to a hole drilled on the upper right side of the front wall among the four walls to supply water from the third filtration chamber to the anaerobic tank;
a mineral material, a sponge filter, and a non-woven fabric filter stacked sequentially from the bottom in the precipitation chamber; and
It is constituted by providing a mineral material to be spread on the floor of the first to third filtration chambers;
The anaerobic tank,
anaerobic tank body formed of a four-sided wall, top and bottom;
a rotating shaft coupled to the upper portion of the anaerobic tank body to rotate the impeller inside the body at a constant speed;
an impeller connected to one end of the rotation shaft to mix water inside the body;
a motor connected to the other end of the rotating shaft to rotate the rotating shaft;
a supply unit connected to the lower end of the left side wall of the four walls of the anaerobic tank and injecting water supplied from the third filtration chamber into the anaerobic tank;
a discharge unit connected to the upper right side of the four walls of the anaerobic tank to discharge the completed anaerobic reaction water and supply it to the aquaculture tank; and
an organic component supply unit for supplying an organic component to the anaerobic tank;
A method of reducing mortality in the process of trout in seawater, characterized in that it comprises a.
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