KR102276371B1 - 조작된 면역조절요소 및 이에 의해 변형된 면역 활성 - Google Patents

조작된 면역조절요소 및 이에 의해 변형된 면역 활성 Download PDF

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KR102276371B1
KR102276371B1 KR1020190068999A KR20190068999A KR102276371B1 KR 102276371 B1 KR102276371 B1 KR 102276371B1 KR 1020190068999 A KR1020190068999 A KR 1020190068999A KR 20190068999 A KR20190068999 A KR 20190068999A KR 102276371 B1 KR102276371 B1 KR 102276371B1
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KR20190075019A (ko
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김석중
김윤영
유호성
정인영
이정민
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주식회사 툴젠
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KR1020190068999A 2016-08-12 2019-06-11 조작된 면역조절요소 및 이에 의해 변형된 면역 활성 Active KR102276371B1 (ko)

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Application Number Priority Date Filing Date Title
KR1020160103308 2016-08-12
KR1020160103308 2016-08-12
US201762502822P 2017-05-08 2017-05-08
US62/502,822 2017-05-08
KR1020170103009A KR20180018457A (ko) 2016-08-12 2017-08-14 조작된 면역조절요소 및 이에 의해 변형된 면역 활성

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Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
BR112019002779A2 (pt) * 2016-08-12 2019-05-14 Toolgen Incorporated elemento imunorregulador manipulado e imunidade alterada por este
US11261428B2 (en) * 2018-03-15 2022-03-01 KSQ Therapeutics, Inc. Gene-regulating compositions and methods for improved immunotherapy
JP7235391B2 (ja) * 2017-05-08 2023-03-08 ツールゲン インコーポレイテッド 人工的に操作された免疫細胞
CN107164377A (zh) * 2017-06-12 2017-09-15 王小平 基于碱基编辑的基因敲除方法及其应用
CA3069296A1 (en) * 2017-07-07 2019-01-10 Toolgen Incorporated Target-specific crispr mutant
CN110904045A (zh) * 2018-09-17 2020-03-24 中国科学院动物研究所 经修饰的t细胞、其制备方法及用途
CN111321171A (zh) * 2018-12-14 2020-06-23 江苏集萃药康生物科技有限公司 一种应用CRISPR/Cas9介导ES打靶技术制备基因打靶动物模型的方法
CN110373385A (zh) * 2019-07-12 2019-10-25 赛德特生物科技开发有限公司 改善神经细胞功能的免疫细胞培养基及其制备方法与应用
US12344656B2 (en) * 2019-09-06 2025-07-01 Crispr Therapeutics Ag Genetically engineered T cells having improved persistence in culture
US12152251B2 (en) * 2020-08-25 2024-11-26 Kite Pharma, Inc. T cells with improved functionality
AU2022220754A1 (en) * 2021-02-10 2023-08-17 Cartherics Pty. Ltd. Methods and compositions for generating stem cell-derived immune cells with enhanced function
CN114807044B (zh) * 2021-04-30 2023-09-08 四川大学华西医院 一种具有高nkt细胞比例的car-cik细胞制备方法及应用
CN115894706A (zh) * 2021-09-30 2023-04-04 北京康辰药业股份有限公司 靶向PD-L1和FasL的融合蛋白、核苷酸序列及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014039513A2 (en) * 2012-09-04 2014-03-13 The Trustees Of The University Of Pennsylvania Inhibition of diacylglycerol kinase to augment adoptive t cell transfer
US20140120906A1 (en) 2011-07-29 2014-05-01 Intsig Information Co., Ltd. Method and system integrating geographical location information and bluetooth technology for relaying electronic business card
WO2015090230A1 (en) 2013-12-19 2015-06-25 Novartis Ag Human mesothelin chimeric antigen receptors and uses thereof
US20160120906A1 (en) 2013-05-13 2016-05-05 Cellectis Methods for engineering highly active t cell for immunotheraphy

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050244851A1 (en) 2004-01-13 2005-11-03 Affymetrix, Inc. Methods of analysis of alternative splicing in human
US20050221354A1 (en) * 2004-02-18 2005-10-06 Wyeth Nucleic acid arrays for monitoring expression profiles of drug target genes
US7901882B2 (en) * 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2013033627A2 (en) * 2011-09-01 2013-03-07 The Regents Of The University Of California Diagnosis and treatment of arthritis using epigenetics
EP3241902B1 (en) 2012-05-25 2018-02-28 The Regents of The University of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
EP2906684B8 (en) * 2012-10-10 2020-09-02 Sangamo Therapeutics, Inc. T cell modifying compounds and uses thereof
CN105121648B (zh) 2012-12-12 2021-05-07 布罗德研究所有限公司 用于序列操纵的系统、方法和优化的指导组合物的工程化
DK2931897T3 (en) * 2012-12-12 2018-02-05 Broad Inst Inc CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS
CA2894684A1 (en) * 2012-12-12 2014-06-19 The Broad Institute, Inc. Engineering and optimization of improved crispr-cas systems, methods and enzyme compositions for sequence manipulation in eukaryotes
CN105683376A (zh) 2013-05-15 2016-06-15 桑格摩生物科学股份有限公司 用于治疗遗传病状的方法和组合物
ES2883131T3 (es) 2013-05-29 2021-12-07 Cellectis Métodos para la modificación de células T para inmunoterapia utilizando el sistema de nucleasa CAS guiado por ARN
WO2014191128A1 (en) 2013-05-29 2014-12-04 Cellectis Methods for engineering t cells for immunotherapy by using rna-guided cas nuclease system
ES2897579T3 (es) * 2013-06-10 2022-03-01 Dana Farber Cancer Inst Inc Métodos y composiciones para reducir la inmunosupresión por células tumorales
ES3063961T3 (en) 2014-02-14 2026-04-21 Cellectis Cells for immunotherapy engineered for targeting antigen present both on immune cells and pathological cells
EP3593812A3 (en) * 2014-03-15 2020-05-27 Novartis AG Treatment of cancer using chimeric antigen receptor
AU2015289644A1 (en) 2014-07-15 2017-02-02 Juno Therapeutics, Inc. Engineered cells for adoptive cell therapy
ES2865275T3 (es) 2014-08-06 2021-10-15 College Of Medicine Pochon Cha Univ Industry Academic Cooperation Foundation Células inmunocompatibles creadas por edición mediada por nucleasas, de genes que codifican HLA
WO2016069282A1 (en) 2014-10-31 2016-05-06 The Trustees Of The University Of Pennsylvania Altering gene expression in modified t cells and uses thereof
WO2016080097A1 (ja) 2014-11-17 2016-05-26 国立大学法人東京医科歯科大学 簡便で高効率の遺伝子改変非ヒト哺乳動物の作製方法
WO2016123578A1 (en) 2015-01-30 2016-08-04 The Regents Of The University Of California Protein delivery in primary hematopoietic cells
EP3280803B1 (en) * 2015-04-06 2021-05-26 The Board of Trustees of the Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
BR112019002779A2 (pt) 2016-08-12 2019-05-14 Toolgen Incorporated elemento imunorregulador manipulado e imunidade alterada por este
KR20180018457A (ko) 2016-08-12 2018-02-21 주식회사 툴젠 조작된 면역조절요소 및 이에 의해 변형된 면역 활성
CN118726313A (zh) 2016-10-07 2024-10-01 综合Dna技术公司 化脓链球菌cas9突变基因和由其编码的多肽
EP3336107A1 (en) 2016-12-15 2018-06-20 Miltenyi Biotec GmbH Immune cells expressing an antigen binding receptor and a chimeric costimulatory receptor
JP7235391B2 (ja) 2017-05-08 2023-03-08 ツールゲン インコーポレイテッド 人工的に操作された免疫細胞
US12467050B2 (en) 2017-10-02 2025-11-11 Georgia Tech Research Corporation Methods and compositions for engineering synthetic bioswitches for remote control of biological activity
CA3084446A1 (en) 2017-12-08 2019-06-13 Juno Therapeutics, Inc. Phenotypic markers for cell therapy and related methods
CN112823011A (zh) 2018-07-09 2021-05-18 加利福尼亚大学董事会 基于t细胞的免疫疗法的基因靶标

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140120906A1 (en) 2011-07-29 2014-05-01 Intsig Information Co., Ltd. Method and system integrating geographical location information and bluetooth technology for relaying electronic business card
WO2014039513A2 (en) * 2012-09-04 2014-03-13 The Trustees Of The University Of Pennsylvania Inhibition of diacylglycerol kinase to augment adoptive t cell transfer
US20160120906A1 (en) 2013-05-13 2016-05-05 Cellectis Methods for engineering highly active t cell for immunotheraphy
WO2015090230A1 (en) 2013-12-19 2015-06-25 Novartis Ag Human mesothelin chimeric antigen receptors and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Cancer Research (2013) 73(12):3566-3577
Genome Biology, Vol.16, Article 251, pp.1-3 (2015)
NCBI, GenBank: EF064716.1 (2006.11.13.)
Science (2013) 341:833-836
Scientific Reports (2016.01.28.) 6:20070(pp.1-13)*
The Journal of Immunology, Vol.196, Issue 1, Supplement, Immunology 2016TM Meeting Abstract, 214.24 (2016.05.01.)

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