KR102155057B1 - Food Composition for blood circulation and preventing blood vessel disease Comprising red ginseng and nitric oxide solution and its manufacturing method - Google Patents

Abstract

The present invention relates to a method for manufacturing a composition for improving blood circulation and preventing blood vessel diseases which contains red ginseng and a nitric oxide solution as active components, and the composition for improving blood circulation and preventing blood vessel diseases manufactured therefrom. An herb medicine composition is manufactured by the method comprising the following steps: (1) mixing red ginseng powder and the nitric oxide solution in a weight ratio of 1 : 5 to 7 times, followed by dispersing and emulsifying the same in a high-speed nano emulsifier to manufacture a fine pulverized red ginseng nitric oxide solution; (2) mixing 30 parts by weight of Hovenia dulcis fruit powder, 25 parts by weight of Cirsium japonicum powder, 25 parts by weight of Alnus japonica powder, and 20 parts by weight of Glycyrrhiza uralensis powder with respects to 300 parts by weight of water, followed by heating the same at 100 to 120°C for 1 to 3 hours to manufacture a hot water extract; (3) adding 20 to 50 parts by weight of the fine pulverized red ginseng nitric oxide solution to the 100 parts by weight of the hot water extract, followed by fermenting the same by using fermentation bacteria; and (4) filtering the fermented product to obtain a clear herb medicine which relaxes blood vessels and improves blood flow to promote blood circulation, thereby being able to be used as a pharmaceutical and food composition which can prevent and alleviate blood vessel diseases such as hypertension.

Description

Food Composition for blood circulation and preventing blood vessel disease Comprising red ginseng and nitric oxide solution and its manufacturing method containing red ginseng and nitric oxide solution as active ingredients

The present invention relates to a method for preparing a composition for preventing blood circulation and vascular disease comprising red ginseng and nitric oxide as an active ingredient, and a method for preparing a composition for preventing blood circulation and vascular disease prepared accordingly, and more specifically, red ginseng And a mixture of dulcis fruit, thistle, alder, and licorice mixed with nitrogen oxide, and preferably a mixture of garlic and argi added as an active ingredient to relax blood vessels and improve blood flow to improve blood circulation. It relates to a method for producing a herbal composition capable of preventing and improving vascular diseases such as hypertension by promoting.

Chronic diseases of the circulatory system, including hypertension, ischemic heart disease, and cerebrovascular disease, are increasing due to the aging population due to rapid economic development and medical development. According to the 2014 Health Insurance Statistical Yearbook, medical expenses for chronic diseases such as high blood pressure, kidney disease, and diabetes were the most used, and medical expenses for the elderly over 65, accounting for more than a third of medical expenses, increased 2.2 times compared to 2007. Likewise, the most medical expenses were used for hypertension. In other words, along with the rapidly progressing population aging, the burden of socioeconomic diseases caused by chronic diseases is expected to increase in the future.

Hypertension refers to a continuous high blood pressure beyond the normal range, and refers to a case where the systolic blood pressure is 140 mmHg or more or the diastolic blood pressure is 90 mmHg or more in adults over 18 years of age. Systolic blood pressure is the pressure applied to blood vessels when the heart contracts and discharges blood, and diastolic blood pressure is the pressure that blood vessels receive when the heart expands (relaxes) and accepts blood. High blood pressure is the most frequently incidence of chronic circulatory system diseases, and although relatively symptomless, it can cause fatal complications such as stroke, heart failure, and coronary artery disease, requiring more active management and treatment. The substance has been reported to have problems related to side effects such as loss of strength in the whole body, reduction of leukocytes, angioedema, liver dysfunction, vomiting, dry cough, headache, loss of appetite, and taste disorders. Therefore, in order to solve this problem, there is an urgent need to develop a natural substance-derived therapeutic agent in which safety is secured without side effects.

Currently, many antihypertensive drugs have been developed and used for the treatment of hypertension, which is one of the adult diseases, and depending on the mechanism and point of action, diuretics, sympathetic drugs (α,2-adrenergic antagonists, β-adrenergic antagonists), vasodilators, calcium It is classified as a calcium channel blockers and angiotensin converting enzyme (ACE) inhibitors (Mann, SJ., Neurogenic essential hypertension revisited: The case for increased clinical and research attention, American Journal of Hypertension, 16, pp.881-888, 2003; Escobales, N., Crespo, MJ., Oxidative-nitrosative stress in hypertension, Current Vascular Pharmachology, 3, pp.231-246, 2005).

ACE inhibitors are a kind of peptidyl dipeptidase. This enzyme catalyzes the production of angiotensin Ⅱ (Ang Ⅱ), which is known as the strongest booster, and at the same time inhibits the breakdown of bradykinin, the most powerful vasodilator. Catalyzes. Ang II, a pharmacologically active substance produced by cleavage of His-Leu at the C-terminus of Ang I, which is inactive by ACE, raises blood pressure through strong vasoconstriction, and acts to preserve sodium by stimulating the release of aldosterone in the adrenal cortex. Increase the volume in blood vessels. In addition, it is known that the six amino acids at the C-terminus of the structure of Ang II have most of the information essential for pharmacological action. Since ACE inhibitors that inhibit the activity of ACE exhibit a consistent blood pressure lowering effect, they can be used over a wide range for the treatment of hypertension, including patients with essential hypertension. In addition, ACE inhibitors not only have a remarkable blood pressure lowering effect, but also have good tolerability. Unlike other antihypertensive drugs, ACE inhibitors have fewer side effects from long-term use, and can be used for hypertensive patients with heart disease, diabetes, asthma, etc., so they are highly useful as antihypertensive agents. (Thurman, JM., Schrier, RW., Comparative effects of angiotensin-converting enzyme inhibitors and angiotensin receptor blockers on blood pressure and the kidney, Am J Med., 114, pp.588-598, 2003).

Currently, many studies on ACE inhibitors are being conducted, and Teprotide was the first ACE inhibitor introduced, but this had shortcomings in that it had a short action time and required vascular injection. Orally useful ACE inhibitors such as Enalapril have been developed and are currently being used as new and powerful antihypertensive agents.

On the other hand, red ginseng (紅蔘) uses ginseng (Panax ginseng CA Meyer) as a raw material, and it is steamed and dried by steam or other methods, and has a sedative and excitable effect on the central nervous system, and acts on the circulatory system. It is effective in preventing high blood pressure or arteriosclerosis. In addition, it lowers the hematopoietic action and blood sugar level, protects the liver, acts on the endocrine system, acts indirectly and effectively on sexual behavior and reproductive effects, and has anti-inflammatory and It has anti-tumor action (抗腫瘍作用), has a protective effect against radiation, and has an action to protect and soften the skin. According to the data presented by the Nutrition and Functional Food Standards Division of the Ministry of Food and Drug Safety, ginseng and red ginseng have functions such as helping blood flow through fatigue recovery, immunity enhancement, memory enhancement, and platelet aggregation inhibition. Was admitted. In addition, it is known to have medicinal effects such as lowering blood sugar, antioxidant activity, and wound healing.

In addition, Nitric Oxide (NO) is a signaling substance that relaxes blood vessels in the cardiovascular system, and can improve blood circulation. Nitric oxide water in which nitric oxide is dissolved is helpful in combating infectious bacteria, viruses, or demodex on the scalp, and is also effective in inflammatory diseases. Such a nitrogen oxide generation technology is known to produce nitrogen oxide by chemically reacting ammonia and nitric acid, and to produce nitrogen oxide at 800 to 1200°C in a group 8 catalyst atmosphere by mixing air with ammonia.

Therefore, the present inventors discovered that red ginseng glycosides and nitric oxide water can be combined with red ginseng glycosides to increase activity to relax blood vessels and improve blood flow, and red ginseng and nitric oxide water can be used as halibut, thistle, and alder. And it succeeded in preparing a herbal composition for preventing blood circulation and vascular disease comprising a mixture of licorice as an active ingredient, thereby completing the present invention.

Therefore, the technical problem to be solved in the present invention is to provide a method of preparing a composition for improving blood circulation and preventing or treating vascular diseases.

In addition, another technical problem to be solved in the present invention is to provide a composition for improving blood circulation and preventing or treating vascular diseases prepared according to the above manufacturing method.

In order to solve the above technical problem, the present invention provides a method for preparing a herbal composition for improving blood circulation and preventing or treating vascular diseases including red ginseng and nitric oxide, including the following steps:

(1) mixing red ginseng powder and nitrogen oxide water in a weight ratio of 1: 5 to 7 times and dispersing and emulsifying in a high-speed nanoemulsifier to prepare a fine pulverized red ginseng nitric oxide;

(2) 25 to 35 parts by weight of walnut fruit powder, 20 to 30 parts by weight of thistle powder, 20 to 30 parts by weight of alder powder, and 15 to 25 parts by weight of licorice powder are mixed with respect to 300 parts by weight of water at 100 to 120°C Heating for 1 to 3 hours to prepare a herbal hot water extract;

(3) adding 20 to 50 parts by weight of red ginseng nitric oxide fine pulverized liquid to 100 parts by weight of the hot water extract, and then fermenting it using a fermentation bacteria; And

(4) filtering the fermented product to obtain a clear herbal composition.

In order to solve the above technical problem, the present invention provides a composition for improving blood circulation and preventing or treating vascular diseases, including red ginseng and nitric oxide prepared according to the above manufacturing method.

Preferably, in the present invention, 10 to 20 parts by weight of garlic powder and 3 to 10 parts by weight of L-arginine are additionally added based on 300 parts by weight of water in step (2).

The nitrogen oxide water used in the present invention inhales air to decompose the covalent bonds of nitrogen (79% occupied) molecules and oxygen (21% occupied) molecules constituting the air by arc discharge, resulting in high concentration of nitrogen oxide (NO) in plasma. Can be prepared by dispersing and emulsifying in water in a high-speed dispersion emulsifier.

According to the present invention, nitric oxide water can be produced by a nature-friendly technology, and in addition to the advantage of being very low in production cost, it has additional functions such as sterilization and deodorization effect of space.

According to the present invention, when red ginseng powder and the nitric oxide water are mixed and dispersed and emulsified in a high-speed nanoemulsifier, numerous nanobubbles are formed, thereby increasing the contact and binding of red ginseng glycosides and nitric oxide to prepare a fine pulverized red ginseng nitric oxide. I can.

The Hovenia plant used in the present invention has about 3 species and 2 mutant species in the world. There are 3 types and 2 mutant types in China, but 3 types are used as medicines. This species is distributed in Hebei, Shandong, Shanxi, Hanam, Shaanxi, Gansuk, Sichuan, Hubei, Anhui, Jiangsu and Gangseo of China, and is also present in the Korean Peninsula and Japan. In the 「Korean Pharmacopoeia Jeon and Foreign Herbal Medicines (Pharmaceuticals) Standards Book」 (4th edition), Earth character is listed as "the fruit or seed of the Hovenia dulcis Thunb." The abbreviation'Earth' was first recorded in 《Shinsu-boncho (新修本草)》. The main production areas are China's Shaanxi, Hubei, Jiangsu, Anhui, and Fujian. The main active ingredients of the serrata are triterpenoid saponin compounds and flavonoids. Through pharmacological research, it is known that the stalk tree has the effects of haeju (解酒), anti-liver damage, anti-lipid peroxidation, and strong blood sugar. In the present invention, the fruit of dulcis is used.

Thistle used in the present invention is a plant called thorny greens, hanggasae, etc., and is a perennial plant growing in the mountains. For thistle wine, wash and dry 400 grams of thistle root, put soju and sugar together in a container, and seal it for 3 months, then remove the ingredients and drink. When using thistle flowers, remove the flower roots, add 4 times the amount of soju and add sugar together. After about 2 months, it can be aged and drinkable. Thistle wine is known to be effective in tonic, dry stomach, food poisoning and detoxification.

The alder tree used in the present invention is Alnus japonica STEUD. It reaches 20m in height, has no hairs or no hairs on the body, has a slightly ridgeline, has a distinctive skin, and has a large [軸] in winter snow. Because of its soft material, it is often used as a material for making wood, such as hamjip and clogs. In bark and fruit, alum is used as a mordant, rhubarb and dark brown, and bark is made of iron and iron as a mordant. It is used to draw dye. In oriental medicine, bark (樹皮) or Noonji (嫩枝) is used as a medicine. Yakseong (凉) and high sap (苦澁), and is known to be effective in antipyretic, hemostasis, and convergence. Mainly, it is used for flesh blood, stool blood, enteritis, diarrhea, and traumatic bleeding.

The scientific name of licorice used in the present invention is Glycyrrhiza uralensis FISCH. The roots are dried and used as a herbal medicine, but because the taste is sweet, it is called licorice. It contains sweet glycyrrhizin and glabric acid, sucrose, glucose, liquiritin, and lycoricidine. Glycyrrhizin detoxifies diphtheria toxin, tetanus toxin, hydrochloric acid codeine, strychnine acetate, snake venom and pufferfish venom, and has anti-inflammatory properties and has an effect of suppressing edema. In addition, it was confirmed that glycyrrhizin has an effect of lowering blood pressure by lowering cholesterol levels in the blood, promoting the secretion of bile, and having antitussive and analgesic effects. As a result of ingesting ulcers in the dog and taking licorice decoction as a digestive system, ulcer suppression was shown. It also showed a significant inhibitory effect on the experimentally induced bladder stones, and in the anticancer activity test, it showed a significant inhibitory effect on ascites cancer and liver cancer. It is also effective in general inflammation, so it has a good therapeutic effect on initial symptoms such as sore throat, stomatitis, and mastitis. When the skin eczema and acne on the face are severe, it is taken as a decoction with gold and silver flowers (金銀花).

According to one embodiment of the present invention, based on 300 parts by weight of water, 25 to 35 parts by weight of walnut fruit powder, 20 to 30 parts by weight of thistle powder, 20 to 30 parts by weight of alder powder, and 15 to 25 parts by weight of licorice powder The mixture is heated at 100 to 120° C. for 1 to 3 hours to prepare and use a herbal hot water extract.

According to another embodiment of the present invention, it is characterized in that it further comprises 10 to 20 parts by weight of garlic powder and 3 to 10 parts by weight of L-arginine based on 300 parts by weight of water.

The garlic reduces blood cholesterol, promotes blood circulation, and helps lower blood pressure.

The L-arginine is known to produce nitrogen monoxide (NO) and act as a powerful vasodilating material to relax arteries and facilitate blood flow.

According to one embodiment of the present invention, 20 to 50 parts by weight of red ginseng nitric oxide fine pulverized liquid is added to 100 parts by weight of the hot water extract, and then fermented using a fermentation bacteria.

The fermentation bacteria used in the present invention include lactic acid bacteria, Bacillus bacteria and yeast bacteria, such as Lactobacillus plantarum and Pediococcus acidilactici ; Bacillus bacteria such as Bacillus subtilis and Bacillus licheniformis ; And yeast such as Saccharomyces cerevisiae and Saccharomyces boulardii may be exemplified, and it is preferable to use a mixed strain obtained by selecting and mixing one strain from each of lactic acid bacteria, Bacillus and yeast. In particular, in the present invention, it is preferable to use a mixed strain of Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae .

Lactobacillus , such as Lactobacillus, produces acids to lower the intestinal pH to inhibit harmful microorganisms, produce substances such as vitamins and various amino acids, dominate the intestinal flora, and inhibit the proliferation of other bacteria. Yeast bacteria such as Saccharomyces It produces nutrient-degrading enzymes, and has the effect of inhibiting the generation of ammonia or enhancing feed palatability of livestock by alcohol or glutamic acid (Helle et al., 1993).

In the present invention, the Saccharomyces cerevisiae is a representative yeast bacterium belonging to associa fungi, and is non-pathogenic, and produces alcohol and carbon dioxide by fermenting polysaccharides or monosaccharides even in stress environments such as salts, ions, and heat. It can be used for a long time in food fermentation.

According to one specific embodiment of the present invention, the fermentation feed additive manufacturing and utilization strain combination is one type of lactic acid bacteria ( Lactobacillus plantarum ), one type of Bacillus ( Bacillus subtilis ) and one type of yeast ( Saccharomyces cerevisiae ), a total of 3 strains were selected.

According to one embodiment of the present invention, the lactic acid bacteria, Bacillus bacteria and yeast bacteria are preferably mixed in a weight ratio of 2: 2: 1.

In the present invention, "blood pressure drop" is used to mean lowering the blood pressure to the normal blood pressure range for a subject whose blood pressure exceeds the normal blood pressure range (80 mmHg to 120 mmHg).

In the present invention, "angiotensin converting enzyme" is an enzyme that converts angiotensin to angiotensinogen with the help of renin, and functions to constrict blood vessels and increase blood pressure.

In the present invention, the vascular disease may be hypertension.

According to one preferred embodiment of the present invention, in order to determine whether the herbal composition according to the present invention improves blood circulation and promotes blood flow, cholesterol levels of mice administered with the composition and a control group without administration were compared. It was confirmed that blood circulation was improved.

According to one preferred embodiment of the present invention, it was confirmed that the herbal composition according to the present invention can prevent an increase in blood pressure by inhibiting the activity of angiotensin converting enzyme.

Therefore, the herbal composition according to the present invention can be used as a pharmaceutical composition and a food composition because it can prevent, treat, and improve vascular diseases such as hypertension by relaxing blood vessels and improving blood flow to promote blood circulation.

The term "prevention" as used herein refers to suppressing the occurrence of a disease or disease in an individual who has not been diagnosed as having a disease or disease, but is prone to such a disease or disease.

The term “treatment” as used herein refers to (a) inhibition of the development of a disease or disease (b) alleviation of the disease or disease and (c) elimination of the disease or disease in an individual.

As used herein, the term "improvement" means any action in which a disease or symptom of a disease is improved in an individual.

The term "individual" as used herein refers to monkeys, cattle, horses, pigs, sheep, dogs, cats, rats, mice, chimpanzees, etc., including humans with diseases that can improve symptoms by administering the composition of the present invention. Means mammals.

When the composition of the present invention is used as a pharmaceutical composition, it may further contain a pharmaceutically acceptable carrier as an active ingredient. Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are commonly used in formulation, and carbohydrate compounds (eg, lactose, amylose, dextrose, sucrose, sorbitol, mannitol, starch, cellulose, etc.) , Gum acacia, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, salt solution, alcohol, gum arabic, vegetable oil (e.g. corn oil, cotton seed oil , Soy milk, olive oil, coconut oil), polyethylene glycol, methyl cellulose, methylhydroxy benzoate, propylhydroxy benzoate, talc, magnesium stearate, mineral oil, etc., but are not limited thereto. The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington's Pharmaceutical Sciences (latest edition), Mach Publishing Company, Easton, PA.

The pharmaceutical composition can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and sterile injectable solutions according to a conventional method.

The pharmaceutical composition may prevent or treat vascular disease, and the treatment method includes administering the pharmaceutical composition in a pharmaceutically effective amount to mammals such as mice, mice, livestock, and humans by various routes. The administration method may be performed in any manner, for example, oral, rectal or intravenous injection, intraperitoneal administration, intramuscular administration, or cerebrovascular administration.

The dosage of the pharmaceutical composition may vary depending on factors such as formulation method, administration method, age, weight, sex, and pathological condition of the patient, and may be appropriately selected by those skilled in the art. In general, the pharmaceutical composition of the present invention may be administered by dividing the amount of 0.001-100 mg/kg once or several times a day on an adult basis. However, the dosage does not limit the scope of the present invention in any way.

As another specific use of the present invention, the composition of the present invention can be used as a food composition.

When the composition of the present invention is used as a food composition, it may further include ingredients that are commonly added, for example, proteins, carbohydrates, fats, nutrients, flavoring and flavoring agents.

Examples of the carbohydrate include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, natural flavoring agents [taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)] and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

In addition to the above-described components, the food composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohols, carbonates used in carbonated beverages, and the like. In addition, the food composition of the present invention may contain pulp for the production of natural fruit juice, beverage and vegetable beverage. These components may be used independently or in combination.

There is no particular limitation on the type of food. Examples of foods to which the ramnocitrine of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea , Drinks, alcoholic beverages, and vitamin complexes, and includes all health foods in the usual sense.

When the food composition of the present invention is prepared as a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, etc. may be additionally included in addition to the ramnocitrin or genquanin of the present invention.

In addition, the food composition of the present invention may additionally contain a food additive, and the suitability as a food additive is determined according to the general rules and general test methods of the Food Additive Code approved by the Food and Drug Administration (KFDA), unless otherwise specified. It is determined according to the standards and standards for the item.

Examples of the items listed in the food additive code include chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid; Natural additives such as crystalline cellulose and guar gum; Sodium L-glutamate formulation; An alkali agent added to noodles; Preservatives; Mixed preparations, such as a tar coloring agent, etc. are mentioned.

On the other hand, the herbal composition of the present invention is a harmless substance without cytotoxicity. Therefore, the ramnocitrin and genquanin of the present invention can be safely used even when used for a long period of time, and can be particularly safely used in pharmaceutical or food compositions as described above.

As described above, the herbal composition comprising a mixture of red ginseng and nitric oxide water of the present invention, as an active ingredient, of dulcis fruit, thistle, alder and licorice as an active ingredient relaxes blood vessels and improves blood flow to improve blood circulation. It can be used as a pharmaceutical and food composition that can prevent and improve vascular diseases such as high blood pressure by promoting.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

<Production Example 1> Preparation of nitrogen oxide water

Nitric oxide water inhales air and dissolves covalent bonds of nitrogen (79% occupied) molecules and oxygen (21% occupied) molecules constituting the air by arc discharge to dissolve high concentration nitrogen oxide (NO) in plasma state at high speed. It was prepared by dispersing and emulsifying in water (using K&S Company's Parson emulsifier).

<Example 1> Preparation of herbal composition

Red ginseng powder and the nitric oxide solution prepared in Preparation Example 1 were mixed at a weight ratio of 1:6 and dispersed and emulsified in a high-speed nanoemulsifier to prepare a red ginseng nitric oxide fine pulverized solution.

Then, 30 parts by weight of dulcis fruit powder, 25 parts by weight of thistle powder, 25 parts by weight of alder powder, and 20 parts by weight of licorice powder were mixed with respect to 300 parts by weight of water, and heated at 110° C. for 2 hours to obtain a hot water extract.

To 100 parts by weight of the obtained hot water extract, 40 parts by weight of the prepared red ginseng nitric oxide finely pulverized solution was added to prepare a total of 6 strains of 2 types of lactic acid bacteria, 2 types of Bacillus bacteria, and 2 types of yeast as shown in Table 1 below. It was selected as a publicly known strain. The identified strains were selected based on criteria such as isolation from the intestine of animals, production of antibiotics or substances that inhibit the growth of microorganisms during growth, and strong digestive substances.

division Microorganism name Badge name Culture method Incubation time Lactobacillus Lactobacillus plantarum
Pediococcus acidilactici MRS Anaerobes culture 48 Bacillus bacteria Bacillus subtilis
Bacillus licheniformis NB Aerobic culture 48 yeast fungus Saccharomyces cerevisiae
Saccharomyces boulardii YM Anaerobes culture 24

As shown in Table 1, the solid and liquid culture medium of lactic acid bacteria in the present invention is MRS medium (Difco, Detroit, USA), Bacillus (Bacillus) Genus NB medium (Nutrient Broth, Difco), Saccharomyces ( yeast) The genus was used YM broth medium (Difco) these culture conditions Lactobacillus genus and Pediococcus genus was 48 hours static culture at 37 ℃, genus Bacillus (Bacillus bacterium) 37 Shaking culture was performed at 150 rpm for 48 hours at ℃, and Saccharomyces (yeast bacteria) was incubated for 24 hours at 30 ℃.

Then, the obtained fermented product was filtered to obtain a clear herbal composition.

<Example 2> Preparation of herbal composition

A herbal composition was prepared in the same manner as in Example 1, except that 10 parts by weight of garlic powder and 3 parts by weight of L-arginine were additionally included in 300 parts by weight of water.

<Test Example 1> Blood flow measurement

After rearing 6-week-old rats (Sprague Dauri, Orient Bio) for 1 week, the general diet group from 7 weeks to 6 weeks was administered a diet composition of the composition shown in Table 2 below (Group 1), and the high-fat diet group (High Fat Diet, HFD 45%) was administered a diet composition of the composition shown in Table 3 (Group 2), and other high fat diet groups (High Fat Diet, HFD 45%) were administered in Examples 1 and 2 together with the diet composition of Table 3 In addition to administering the herbal composition of 100 mg/kg in an amount of 100 mg/kg (Groups 3 and 4), and measuring total cholesterol (TC), triglyceride (TG), HDL-cholesterol, and LDL-cholesterol levels in each group In vivo experiments were performed, and the results are shown in Table 4 below.

Group 1 Group 2 Group 3 Group 4 TC 59.23 90.21 61.24 58.54 TG 134.23 352.76 142.2 134.7 HDL 25.21 32.21 35.23 36.45 LDL 8.34 11.23 6.23 5.56

As shown in Table 4, in groups 3 and 4 to which the composition of the present invention was administered, the total cholesterol level was lowered compared to the high-fat diet group (group 2), and the HDL-cholesterol increased while the LDL-cholesterol was significantly lowered. I could confirm that.

<Test Example 2> Blood circulation improvement effect evaluation test

The herbal composition of Examples 1 and 2 was administered to a total of 10 males and females with blood circulation disorders over 40 years of age, in an amount of 2.75 twice a day for 1 week, and the effect of improving blood circulation was evaluated. And the results are shown in Table 5.

Has a clear effect In effect so so no effect Side Effect The herbal composition administration group of Example 1 8 people 1 person 1 person 0 people 0 people Herbal drug composition administration group of Example 2 9 people 1 person 0 people 0 people 0 people

As shown in Table 5, it can be seen that the herbal compositions prepared in Examples 1 and 2, particularly Example 2, of the present invention provide excellent blood circulation improvement effects.

<Test Example 3> Evaluation of angiotensin converting enzyme (ACE) inhibitory activity

The evaluation of ACE inhibitory activity was analyzed using the previously described method (lee et al., statistics and data analysis method, hyoil press (1998), 253-296). 0.2M boric buffer (pH8.3) and 0.05M sodium borate buffer containing 0.4M NaCl each 50 µl and 100 µl Hip-His-Leu (Sigma, A3-P4DQ11, USA) of the herbal compositions of Examples 1 and 2 Was dissolved in, and incubated with 150 µl of ACE for 1 hour at 37°C. After the reaction was allowed to stand at room temperature for 5 minutes, 250 µl of 1N HCL was added to terminate the reaction.

Hippuric acid was extracted by adding 1.5 µl ethyl acetate. After centrifugation at 3000 rpm for 10 minutes, 1 μl of the upper layer was transferred to a glass tube and dried at 120° C. for 30 minutes. Then, it was allowed to cool in desiccators for 20 minutes. After adding 3 µl of distilled water to the dried sample, the mixture was evenly stirred for 30 seconds. A spectrophotometer (UV-1650PC, Shimadzu, Kyoto, Japan) was used for absorbance at 228 nm. ACE used in this example was treated with 0.2M boric buffer (pH 8.3) and 0.05M sodium borate buffer containing 10 µl of 0.4M NaCl from 1g rabbit lung acetone powder (Sigma, L0756, USA) at 4°C for one day. And extracted by centrifugation (4000rpm, 1h). Aspirin was used as a positive control, and ACE inhibitory ability was calculated through Equation 1 below.

In the above formula,

Sa: sample absorbance, Sb: sample blank absorbance, Ca: control absorbance, Cb: control blank absorbance.

The above values represent the average of the experimental values tested three times, and the data were analyzed by ANOVA and Duncan's multi-range test. Statistically, it was interpreted as having statistical significance when P<0.05.

As a result, the ACE inhibitory ability of the aspirin and the herbal compositions of Examples 1 and 2 were measured to be 30.7±0.57%, 82.7±0.22%, and 85.5±0.12%, respectively. IC ₅₀ of the aspirin and the herbal compositions of Examples 1 and 2, respectively, were measured to be 150.0 µg/µl, 4.0 µg/µl, and 3.4 µg/µl, respectively. From this, it was confirmed that the herbal composition according to the present invention showed superior ACE inhibitory ability than aspirin.

<Test Example 4> Toxicity test

This experiment was carried out to investigate the toxicity to the animal body acutely (within 24 hours) when ingesting an excessive amount of the herbal composition of Example 1 of the present invention in a short period of time, and to determine the mortality rate. 30 ICR mouse strains, which are general mice, were assigned to the control group and the experimental group, respectively, 10 mice. Only PEG-400/tween-80/EtOH (8/1/1, v/v/v) was administered to the control group, and the experimental group used the herbal composition of Examples 1 and 2 of the present invention as the PEG-400/tween-80. /EtOH (8/1/1, v/v/v) was dissolved and administered orally, respectively. As a result of examining each mortality 24 hours after administration, it was confirmed that all mice survived in the control group and in the experimental group to which the herbal composition of Examples 1 and 2 of the present invention was administered at a concentration of 2g/kg/day.

Hereinafter, examples of preparations for the composition of the present invention are illustrated.

<Formulation Example 1> Preparation of healthy food

Composition of Example 1 or 2 ........................... 200 mg

Vitamin mixture ......................................

Vitamin A Acetate ................................70 ㎍

Vitamin E .......................................... 1.0 mg

Vitamin B1 ..........................................0.13 mg

Vitamin B2 ................................0.15 mg

Vitamin B6 .......................................... 0.5 mg

Vitamin B12 ......................................... 0.2 ㎍

Vitamin C ............................................. 10 ㎎

Biotin ............................................... 10 ㎍

Nicotinic acid amide ...................................... 1.7 mg

Folic acid ................................................. 50 ㎍

Calcium pantothenate ....................................... 0.5 mg

Inorganic mixture ........................................ Appropriate amount

Ferrous sulfate ........................................... 1.75 mg

Zinc oxide ............................................0.82 mg

Magnesium carbonate ........................................25.3 mg

Potassium Phosphate Monobasic ..........................................15 ㎎

Dicalcium Phosphate ..........................................55 ㎎

Potassium citrate ........................................... 90 mg

Calcium carbonate ............................................100 mg

Magnesium chloride ........................................ 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for health food, but it may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. , To prepare granules, and can be used to prepare a health food composition according to a conventional method.

<Formulation Example 2> Preparation of healthy beverage

Composition of Example 1 or 2 ............200 mg

Citric acid ...................................1000 mg

Oligosaccharide............................100 g

Plum concentrate ............................................ 2 g

Taurine ................................................ 1 g

Add purified water .............. Total 900 ml

After mixing the above ingredients according to a conventional health drink manufacturing method, stirring and heating at 85°C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 liter container, sealed and sterilized, and then stored in a refrigerator. It is used in the manufacture of health beverage composition.

The composition ratio is composed of ingredients suitable for a relatively preferred beverage in a preferred embodiment, but the mixing ratio may be arbitrarily modified according to regional and ethnic preferences, such as the demand class, the country of demand, and the purpose of use.

As described above, specific parts of the present invention have been described in detail, and it is clear that these specific techniques are merely preferred embodiments, and the scope of the present invention is not limited thereto for those of ordinary skill in the art. Therefore, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (7)

A method of preparing a composition for improving blood circulation or preventing or treating vascular disease comprising red ginseng and nitric oxide comprising the following steps:
(1) Mixing red ginseng powder and nitrogen oxide water at a weight ratio of 1: 5 to 7 times and dispersing and emulsifying in a high-speed nano emulsifier to prepare a fine pulverized red ginseng nitric oxide solution
(2) 25 to 35 parts by weight of walnut fruit powder, 20 to 30 parts by weight of thistle powder, 20 to 30 parts by weight of alder powder and 15 to 25 parts by weight of licorice powder and 10 to 20 parts by weight of garlic powder based on 300 parts by weight of water Mixing parts and 3 to 10 parts by weight of L-arginine and heating at 100 to 120° C. for 1 to 3 hours to prepare a herbal hot water extract;
(3) adding 20 to 50 parts by weight of red ginseng nitric oxide fine pulverized liquid to 100 parts by weight of the hot water extract and fermenting using lactic acid bacteria; And
(4) filtering the fermented product to obtain a clear composition.
The method of claim 1,
The nitrogen oxide water inhales air to dissolve covalent bonds of nitrogen (79% occupied) molecules and oxygen (21% occupied) molecules constituting the air by arc discharge to disperse and emulsify high concentration nitrogen oxide (NO) in plasma state at high speed. A manufacturing method characterized in that it is prepared by dispersing and emulsifying in water in a group. delete delete The method of claim 1,
The lactic acid bacteria is Lactobacillus plantarum ( Lactobacillus plantarum ) Or Pediococcus acidilactici ( Pediococcus acidilactici ), characterized in that the manufacturing method.
The method of claim 1,
The manufacturing method, characterized in that the vascular disease is hypertension.
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