KR102433586B1 - Food composition for relieving hangover and improving hepatic function - Google Patents

Abstract

The present invention relates to a beverage composition for relieving a hangover and improving liver function, comprising a plant mixed extract of dermis, garlic root, goji berry and dandelion, milk thistle extract and calcium nano collagen peptide, and by mixing each component in a specific ratio, It aims to relieve hangover and improve liver function.

Description

Food composition for relieving hangover and improving liver function {Food composition for relieving hangover and improving hepatic function}

The present invention relates to a food composition for relieving hangover and improving liver function.

When consumed in moderation, alcohol promotes metabolism and is a means of relieving stress. However, excessive intake of alcohol can cause thirst, general malaise, fatigue, memory loss, bloating, indigestion, vomiting, diarrhea, and vitamin deficiency. suffer from hangovers.

This hangover phenomenon is known to be caused by the action of alcohol and acetaldehyde accumulated in liver cells and the body. Ingested ethanol is absorbed through the digestive tract, reaching peak blood levels between 20 and 120 minutes after ingestion. Absorbed ethanol is metabolized in all organs including the liver, some excreted through respiration, urine or sweat. Ethanol entering the liver is converted into acetate by the action of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the cytoplasm, which is excreted out of the liver cells through the circulation.

Acetaldehyde, the first metabolite of ethanol, has been found to be the main cause of alcoholic liver disorders because it is highly reactive and more toxic than ethanol. It has been found that acetaldehyde interferes with the respiration of mitochondria, an energy-generating organ within cells, inhibits oxidative phosphorylation, and exhibits immunological cytotoxicity to inhibit the release mechanism of hepatocyte-secreted proteins. In addition, acetaldehyde promotes collagen synthesis in myofibroblasts, causing liver fibrosis and degenerative enlargement of hepatocytes, and also forms adducts by reacting with macromolecules in vivo. In particular, among aldehyde dehydrogenases, type II present in mitochondria is important for the removal of acetaldehyde. Asians generally lack or lack the type II enzyme, so acetaldehyde oxidation is slow, and thus, the toxic action of unoxidized acetaldehyde and ethanol is critical. This disrupts the normal metabolism and causes various hangover phenomena.

The liver is a central organ that metabolizes carbohydrates, proteins, and fats, and is an important organ responsible for excreting substances into the body through oxidation, reduction, hydrolysis and conjugation reactions. However, the liver is also known as the 'silent organ', as liver disease is discovered only after a significant deterioration in pain or subjective symptoms in the early stages.

Most of the causes of liver damage are hepatitis virus (A, B, C, etc.), alcohol (drinking), and non-alcoholic fatty liver, drugs or toxic hepatitis, and there are other rare causes. According to statistics from the Health Insurance Review and Assessment Service, the cost of treatment for alcoholic liver disease in Korea is increasing every year. Also, analyzing the statistics of a domestic life insurance company, the number of deaths from alcoholic liver disease among the causes of death increased seven times compared to 10 years ago. Alcoholic liver disease caused by continuous alcohol intake is emerging as a social problem worldwide, including in Korea. In particular, if alcoholic fatty liver and hepatitis continue, liver fibrosis and cirrhosis of the liver reach a stage where recovery is no longer expected. It remains the only treatment option.

Among liver diseases, diseases caused by chronic drinking are largely divided into alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis, but it is rare that only one pure disease appears in one person, and each disease appears to varying degrees for each individual. It is the most common cause of cirrhosis in the United States and the second most common cause of cirrhosis in Korea after viral hepatitis.

On the other hand, searching for new substances that can be used as health functional foods that can prevent or treat human diseases or improve various functions from natural products has been the subject of research by many scientists from the past. Through the development of such natural resources, more than 50% of currently used medicines are derived from natural products, and there are about 120 types of natural medicines approved by the US FDA, and their market size is about 10 trillion dollars.

For hangover relief, Korean Patent Laid-Open No. 2002-0081995 (Hangover Relief and Liver Function Improvement Agent and Method for Manufacturing the Same by Formulated Food), Republic of Korea Patent Publication No. 2002-0064151 (with hepatotoxicity and hangover relieving activity isolated from H. In patents such as lower alcohol insoluble extract fractions and polysaccharide substances and compositions containing them), various plant-derived preparations such as rice nun extract (gurume), alder, oak wood vinegar, brown flower, and milk thistle extract are effective for hangovers. Although it is reported that there is, it is known that these are effective or insignificant in only some of the various changes induced by drinking.

Therefore, in the direction of the development of functional food related to liver health, it is advantageous to develop a complex formulation of natural products that have effects on each factor that causes liver disease, rather than foods made from a single natural product that helps maintain liver health. To this end, it can be said that it is necessary to evaluate the activity of each liver disease factor by targeting many natural products.

The inventors of the present invention, while studying various physiological activities of plants, confirmed that a composition containing a specific plant mixed extract, milk thistle extract and calcium nano-collagen peptide is effective in relieving hangover and improving liver function. was completed.

Korean Patent Laid-Open Publication No. 10-2002-0081995 Korean Patent Laid-Open Publication No. 10-2002-0064151

The present invention includes a plant mixed extract of dermis, ginseng root, goji berry and dandelion, milk thistle extract and calcium nano collagen peptide, and by mixing each component in a specific ratio, it aims to relieve hangover and improve liver function.

In addition, it aims to relieve a hangover and improve liver function safely in the human body by using a natural product as an active ingredient.

Including plant mixed extracts of dermis, ginseng root, goji berry and dandelion, milk thistle extract and calcium nano collagen peptide, compared to the total weight of the composition

The plant mixed extract is 70 to 90 parts by weight,

The milk thistle extract is 1 to 10 parts by weight,

The calcium nano-collagen peptide provides a food composition for relieving a hangover and improving liver function, characterized in that 0.3 to 5 parts by weight of the composition, more specifically, a beverage composition.

The present invention provides the effect of quickly relieving a hangover and improving liver function by mixing the plant extract, milk thistle extract, and calcium nano collagen peptide of dermis, garlic root, goji berry and dandelion in a specific ratio.

In addition, the present invention has a safe effect on the human body by using a natural product as an active ingredient.

Hereinafter, the present invention will be described in more detail to help the understanding of the present invention. At this time, the terms or words used in the present specification and claims are not to be construed as being limited to conventional or dictionary meanings, and the inventor appropriately defines the concept of the term in order to explain his invention in the best way. It should be interpreted as meaning and concept consistent with the technical idea of the present invention based on the principle that it can be done.

The present invention relates to a food composition for relieving a hangover and improving liver function, and an object of the present invention is to prevent and improve various symptoms caused by a hangover, and to improve liver function.

Specifically, the food composition for relieving hangover and improving liver function of the present invention may include a plant mixed extract of dermis, ginseng root, goji berry and dandelion, milk thistle extract and calcium nano collagen peptide.

The plant mixed extract of the present invention has the effect of relieving a hangover, preventing fatty liver, and improving liver function by promoting blood circulation, water metabolism, alcohol decomposition, detoxification, headache prevention, diuretic action, mental and physical stability, and stress relieving action. there is

Specifically, the dermis refers to the mature pericarp of Citrus unshiu Markovich or a related plant of the rutaceae family. The dermis releases qi clumping, strengthens the function of the spleen, and acts as a diuretic. In addition, it is rich in vitamin C, which promotes metabolism, promotes blood circulation, and helps to recover from fatigue. Effect, capillary protection and anticancer action, cholesterol lowering effect. In addition, the dermis extract has an effect on hepatocellular protection and alcohol breakdown by alcohol.

Also, Pueraria lobata Ohwi, a perennial plant, does not freeze to death even in winter, and most of its stems survive. It is classified as a tree because its trunk grows thicker every year to form a thick trunk. In oriental medicine, the root is used as a medicinal herb called galgeun (葛根), which is effective in relieving sweating, fever, and hangover. As it contains abundant daidzin, puerarin, and catechin components, it has the effects of improving liver function, restoring liver function, relieving hangover, and detoxifying.

In addition, goji (Lycium dhinense Mill) is rich in betaine, one of the choline metabolites, which helps to inhibit the accumulation of fat in the liver, thereby preventing fatty liver. and helps in relieving fatigue. In addition, it increases the activity of alcohol dehydrogenase (ADH), an enzyme that decomposes alcohol, and aldehyde dehydrogenase (ALDH), an enzyme that decomposes acetaldehyde, thereby promoting alcohol decomposition.

In addition, a component called luteolin, which is abundantly contained in dandelion (Taraxacum platycarpum), has anti-inflammatory action and is effective in controlling inflammation. Rich in C, it is effective in relieving fatigue, antioxidant action, and alleviating inflammation in the blood caused by hangover and liver failure. In addition, consumption of dandelion extract relieves fatty liver caused by drinking, improves liver function, and increases the activity of aldehyde dehydrogenase (ALDH), an acetaldehyde decomposing enzyme, to decrease the concentration of acetaldehyde in the blood.

On the other hand, the plant mixed extract of the present invention is characterized in that it exhibits an optimal effect of relieving hangover and improving liver function by mixing and extracting plants in an appropriate ratio in consideration of the efficacy of each component.

Specifically, the dermis of the present invention, galgeun, goji berry and dandelion may be mixed in a weight ratio of 5:5:4:4.

On the other hand, the plant mixed extract may be extracted by vacuum high temperature extraction, hot water extraction, reflux extraction, hot water extraction, cold extraction, room temperature extraction, ultrasonic extraction or steam extraction method, but is not limited thereto.

Specifically, the plant mixed extract of the present invention can be obtained by repeatedly extracting the mixed plant once or several times or more with one or more solvents selected from the group consisting of water and C1 to C4 linear or branched alcohols, more specifically As such, it is preferable that the natural product mixture extract is repeatedly extracted several times for about 10 hours or more at about 100° C. with about 5 to 10 times the amount of water and/or 50% ethanol of the natural product mixture raw material.

The extract is a known method used for separation and extraction of plant components, such as extraction with an organic solvent (alcohol, ether acetone, etc.), distribution of hexane and water, and a method by column chromatography according to a conventional method alone or suitable It can be used after fractionation or purification using a combination method.

In addition, the beverage composition of the present invention is characterized in that it comprises a milk thistle extract.

Milk Thistle is a kind of milk thistle, a medicinal plant of the Asteraceae family that blooms purple flowers. The fruit and seeds contain flavonolignans, and the main constituents are collectively called Silymarin. Silymarin is a very strong anti-toxic substance that protects the liver by removing toxic substances from the liver and improves liver function. Milk thistle is also effective in neutralizing and detoxifying alcohol, relieving liver disease, protecting liver cells from free radicals, and enhancing immune function. Helps to keep the liver healthy by repairing and regenerating liver tissue.

Glutathione is a protein (tripeptide) composed of three amino acids combined with glycine, glutamate, and cysteine. As the most water-soluble and powerful antioxidant produced by the liver, it is the main ingredient with anti-cancer and anti-aging nutrients. In general, the concentration of glutathione is the highest in the liver where toxic substances and alcohol are metabolized, and toxic substances are metabolized and excreted in bile. Glutathione can protect the liver from excessive drinking by removing free radicals from the liver and cells to prevent cell damage.

Therefore, milk thistle can be widely used to treat all types of chronic liver disease, especially fatty liver caused by regular drinking of alcoholic beverages.

In addition, the beverage composition of the present invention is characterized in that it contains calcium nano-collagen peptide.

The calcium nano-collagen peptide may be a nano-collagen peptide represented by the following Chemical Formula 1.

[Formula 1]

(R represents the side chain that determines the type of amino acid, and M is Ca.)

The calcium nano-collagen peptide of the present invention is a complex in which nano-unit collagen peptides and calcium, which is a mineral, are chelated, and can be used in food, medicine, quasi-drugs, cosmetics or feed, etc. In this case, it improves the preservation and stability of the product, and improves skin Alternatively, there is an effect of improving the absorption rate of calcium chelated to collagen peptides as well as peptides in the body.

The calcium nano-collagen peptide is prepared by: 1) adding an enzyme to collagen to hydrolyze it to form a collagen peptide having a nano-unit size; and 2) adding calcium to the collagen peptide to form a calcium nano-collagen peptide chelate.

Collagen, also called hard protein or collagen, is widely distributed in multicellular animals such as invertebrates and vertebrates and is the most quantitatively found hard protein in the human body. It is mainly present in the cornea, bones and skin, and plays a very important role as a component of the dermis layer in the skin. It is known that the main function of collagen is the firmness of the skin, the resistance and bonding of tissues, and the support of cell adhesion.

Collagen can be largely divided into animal collagen such as cow skin and pig skin, vegetable collagen such as fruits, vegetables, seeds, nuts and mushrooms, and marine collagen such as seaweed, fish, fish scales, and fish skin, and production of calcium nano collagen peptide of the present invention Collagen used for this is characterized in that at least one selected from the group consisting of animal collagen, vegetable collagen, and marine collagen.

Collagen has a triple helix structure in which three polypeptide chains are twisted, and the average molecular weight of collagen is 300,000 Da (Dalton, Dalton), which is high. In order to increase the absorption rate of collagen having a large molecular weight in the body, the calcium nano-collagen peptide of the present invention is prepared through a step of decomposing the collagen into nano-unit collagen peptides by adding an enzyme to hydrolyze the collagen.

The collagen peptide of the present invention has an average molecular weight of 200 Da to 600 Da, more specifically 300 Da to 400 Da, and a size of 0.1 nm to 10 nm, more specifically 1 nm to 5 nm. Due to its small molecular weight and small size, it has the effect of being rapidly absorbed into the body or skin.

Here, the enzyme used in the collagen nano-forming step is a proteolytic enzyme, characterized in that it comprises at least one selected from the group consisting of collagenase, trypsin, papain, pepsin, and alcalase, and converting collagen into collagen peptides. The decomposing hydrolysis reaction is characterized in that it is carried out at 50 °C to 70 °C, more specifically, at 50 °C to 60 °C for 2 hours to 24 hours.

When the hydrolysis reaction occurs under the conditions of temperature and time within the above range, it is possible to obtain a collagen peptide having an appropriate molecular weight and size having a high absorption rate in the body.

After the collagen nano-ing step, 2) calcium nano-collagen peptide chelate can be prepared by adding calcium to the collagen peptide obtained through 1) step.

The chelate refers to a bond when a ligand having a plurality of coordination groups for coordinating a metal ion in one molecule is coordinated with a metal, or a complex formed by the bonding. In the present invention, the lone pair of electrons that do not participate in the covalent bond of the amino group bonded to the alpha carbon of the amino acid forms a complete coordination bond with the mineral to form a calcium nano-collagen peptide chelate.

Calcium used in the present invention is calcium carbonate, calcium chloride, calcium lactate, calcium citrate, calcium gluconate, calcium glycerophosphate, calcium oxide, calcium hydroxide, tricalcium phosphate, dibasic calcium phosphate, calcium sulfate, and other food ingredients. It may be manufactured and processed to supplement the.

Calcium used is characterized in that 1 to 20 parts by weight based on 100 parts by weight of the collagen peptide, and within the above range, calcium nano-collagen peptides can be obtained with high purity without unreacted calcium.

In the calcium nano-collagen peptide, collagen is hydrolyzed into nano-sized peptides, so the bioabsorption rate and utilization rate can be maximized compared to conventional collagen.

In addition, it increases the absorption rate of calcium in the body and helps to maximize the bioavailability. When used in food, calcium helps the formation of bones and teeth by supplying calcium in the body, strengthens alveolar bone to keep gums healthy, maintains nerve and muscle functions, reduces the risk of osteoporosis, and balances the potential difference of cell membranes It can help with vascular disease and heart disease.

In addition, the calcium nano-collagen peptide of the present invention can maximize the amount of calcium reaching the small intestine by tightly binding the collagen peptide and calcium through coordination covalent bonding, thereby preventing anion binding and competitive absorption. Furthermore, since absorption is possible without being ionized by enzymes, it can be absorbed not only in the upper part of the small intestine but also in the entire small intestine. In addition, fat solubility can be secured through binding with collagen peptide, and thus can be absorbed from the entire cell membrane, thereby maximizing the amount of calcium absorbed into cells from the small intestine. Furthermore, by minimizing the effect of vitamin D, calcium absorption can be made stably.

In the present invention, the calcium nano-collagen peptide serves as a mineral supply, ion balance in the body, and serves as a high-quality protein source to stabilize nerves, protect gastric mucosa, relieve hangover, and improve liver function.

Here, the beverage composition of the present invention is characterized by mixing the plant extract, milk thistle extract, and calcium nano collagen peptide of the dermis, garlic root, goji berry and dandelion in a specific weight ratio, and specifically, the composition of the present invention is suitable for a hangover. It can be appropriately adjusted depending on the severity of symptoms, the degree of symptom progression, and the health of the liver, but the amount of the plant mixed extract is 70 to 90 parts by weight, specifically 70 to 80 parts by weight, relative to the total weight of the composition, and the milk thistle extract is 1 to 10 parts by weight, specifically 1 to 5 parts by weight, the calcium nano-collagen peptide is most preferably 0.3 to 10 parts by weight, specifically 0.3 to 5 parts by weight.

When each component is included within the above range, an optimal hangover relief and liver function improvement effect can be obtained. When the plant mixed extract is included in less than the above range or the milk thistle extract is included in less than the above range, the alcohol decomposition effect is somewhat lowered, so the effect of relieving a hangover or improving liver function may be insignificant.

On the other hand, the beverage composition of the present invention may further include one or more selected from the group consisting of turmeric extract, guarana extract, naringin extract and hesperidin extract, specifically, turmeric extract, guarana extract, naringin extract and hesperidin extract. can do. More specifically, the composition of the present invention contains 0.01 to 0.1 parts by weight of the turmeric extract, 0.1 to 0.5 parts by weight of the guarana extract, 0.01 to 0.1 parts by weight of the naringin extract, and 0.1 to 0.5 parts by weight of the hesperidin extract relative to the total weight of the composition of the present invention. do it with

Turmeric (Curcuma longa) contains the yellow pigment Curcumin, as well as components such as Turmerone, zingiberene, and PTMC with bile secretion. Turmeric is effective in relieving hangover, promoting liver detoxification, diuresis, anti-bleeding, antibacterial, anti-ulcer, and blood cholesterol suppression. is helpful for

Guarana is used as a raw material for soft drinks, energy drinks, hangover relieving drinks, and tonics because of its high caffeine content and excellent arousal effect. Because guarana contains twice as much caffeine as coffee beans, like other caffeinated plants, the high caffeine concentration in guarana acts as a protective toxin, protecting the guarana fruit or seed from other pathogens. plays a role

Naringin and Hesperidin are flavonoid derivatives that restore liver toxicity through bacteriostatic and antibacterial action, antioxidant action, ascorbic acid synergism, anticancer action, and anti-ulcer (protection from alcohol) action, thereby detoxifying heavy metals. It can also be used as a flavor enhancer in food.

In addition, the beverage composition of the present invention may further include one or more amino acids selected from the group consisting of taurine, histidine, arginine, lysine, glycine, threonine, glutamine, methionine and asparagine, specifically taurine, methionine and asparagine all When included, it has an excellent effect on improving liver fatigue. Taurine is mainly used as a main ingredient in drink products such as nourishing tonics or fatigue recovery agents. It has an excellent effect on improving alcoholic liver disease. Methionine reduces fat in the liver and protects the kidneys. Asparagine produces enzymes that break down alcohol in the liver. It has an excellent effect on hangover.

In addition, the beverage composition of the present invention further contains one or more sugars or sugar alcohols selected from the group consisting of dextrose, sucrose, lactose, sucralose, honey, fructooligosaccharide, inositol, sorbitol, mannitol, maltitol, xylitol, cyclodextrin and erythritol. It may include, specifically, honey, fructooligosaccharide and may further include inositol.

In the present invention, the honey, fructooligosaccharide and inositol can be used as a sweetener, and can compensate for the lack of glucose in the blood due to vomiting and fatigue.

In addition, the beverage composition of the present invention may further include calcium citrate and anhydrous citric acid. Calcium citrate and anhydrous citric acid are mainly used as food additives such as calcium fortifiers or buffers, acidity regulators, acidulants, pH regulators, antioxidants, fruit flavor supplements, preservation enhancers, or acidulants in food. It is effective and helps in recovering from fatigue by supplying minerals.

In addition, the beverage composition of the present invention may further include at least one selected from the group consisting of vitamins B ₁ (thiamine), B ₂ (riboflavin), B ₃ (nicotinamide) and B ₆ (pyridoxine).

The vitamin B complex is water-soluble vitamins that play an important role in cellular metabolism. Specifically, it strengthens the immune system and nervous system functions, maintains muscle health, and has the effect of accelerating metabolism, thus promoting alcohol metabolism and helping to balance water in the body.

The beverage composition of the present invention may include other active ingredients and/or food supplementary additives in addition to the active ingredients, and may further include suitable carriers, excipients and diluents commonly used in the manufacture of beverages. have.

That is, nutrients, vitamins, electrolytes, flavoring agents, colorants, thickeners, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, sweeteners, preservatives, glycerin, alcohol, carbonated beverages It may further contain a carbonation agent, etc. used for The above components may be added independently or in combination.

In addition, the present invention provides a beverage for relieving hangover and improving liver function, comprising the composition for beverage.

The beverage composition of the present invention may be included in a ratio of 1 g to 15 g, specifically 1 g to 10 g, based on 25 ml of the total volume of the beverage, and the remainder may include purified water. The recommended intake can be up to 1 to 2 times a day, 1 package per time.

As an intake method, the beverage composition may be prepared as a liquid beverage and consumed or diluted to a low concentration and consumed instead of water.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

Preparation Example 1

A plant mixture of the composition of Table 1 was prepared to prepare a plant mixture extract. Specifically, each raw material was purchased from Gyeongdong Market (Seoul, Jegi-dong) and used as an extraction raw material. Purified water was added to each of the extraction raw materials, and after extraction at about 100° C. for about 12 hours or more, filtration was performed to obtain a liquid plant mixed extract, and the extraction yield was about 75%.

ingredient weight% dermis 5.0 craving 5.0 Goji berries 4.0 dandelion 4.0 Purified water 82.0 Sum 100.0

Preparation 2

Fish skin and marine collagen of jellyfish were washed with purified water to remove impurities, and 4 liters of purified water was added to 1 kg of raw material and crushed to 50 μm with a chopper.

After that, the raw material deodorized by passing ozone is put into a 0.5% solution of citric acid, and 0.5 w% of pepsin protease is added and hydrolyzed at 55° C. for 24 hours to obtain a molecular weight of 300 Da to 400 Da, size 1 nm to 5 nm Collagen peptides were obtained.

For the preparation of calcium nano-collagen peptide, calcium carbonate was added so that the calcium content was 1 to 20 parts by weight relative to 100 weight of the collagen peptide, followed by enzymatic hydrolysis at 55 ° C., followed by chelation reaction, and then using a spray dryer and dried to prepare a chelate complex powder of calcium nano-collagen peptide.

Example 1

Additional raw materials were mixed with the plant mixed extract of Preparation Example 1 and the calcium nano-collagen peptide of Preparation Example 2 to prepare a beverage composition for relieving hangover and improving liver function of the composition in Table 2 below.

ingredient weight% plant blend extract 76.12 milk thistle 1.45 Calcium Nano Collagen Peptide 0.40 Taurine 0.30 L-Methionine 0.20 L-Asparagine 0.20 turmeric extract 0.01 Guarana Extract 0.40 Naringin 0.07 hesperidin 0.40 Purified water 20.45 Sum 100.0

Comparative Example 1

A beverage composition was prepared in the same manner as in Example 1, except that the plant mixed extract was not mixed in Example 1.

Comparative Example 2

A beverage composition was prepared in the same manner as in Example 1, except that the milk thistle extract was not mixed in Example 1.

Comparative Example 3

A beverage composition was prepared in the same manner as in Example 1, except that the calcium nano-collagen peptide was not mixed in Example 1.

Comparative Example 4

A beverage composition was prepared in the same manner as in Example 1, except that in Example 1, 60 parts by weight and 1.0 parts by weight of the plant mixed extract and milk thistle extract were mixed with respect to the total weight of the composition.

Experimental Example 1: Hangover Relief and Liver Function Improvement Animal Experiment

1-1. Preparation of animal experiments

18 6-week-old ICR (Institute of Cancer Research) mice were acclimatized to a laboratory under the conditions of 22±3 ℃ temperature, 50±20% relative humidity, and 150-300 Lux illumination for 7 days, and then tested when they reached 7 weeks of age. was used for During the adaptation period, reverse osmosis ultrapure water using R/O system was used as drinking water, and radiation sterilized feed (Purina) was freely consumed as feed. made to be The test animals consisted of 3 animals per group of 6 groups of the control group (saline), Examples 1 and 4, so that the average body weight of each group was distributed as evenly as possible by a randomized method on the day of administration. The tail marking method was used for individual identification during the test.

1-2. Statistical processing and analysis

The statistical significance of the results of body weight and serum analysis (ethanol, acetaldehyde, CYP2E1) was analyzed by student's t-test for variation from the control value, and was determined to be statistically significant when the p value was less than 0.05. did. Specifically, a difference in significance was determined by indicating * when the p value was less than 0.05 and † when the p value was less than 0.01.

1-3. Check hangover improvement effect

Ethanol (Sigma-Aldrich Korea) was diluted in distilled water to prepare a 60% ethanol solution. It was prepared just before administration, so no separate analysis was performed on stability and purity.

In order to eliminate the difference in alcohol absorption and degradation by diet, they were fasted 18 hours before test substance administration. Three mice were used in each group.

Specifically, saline, the beverage compositions of Examples 1 and Comparative Examples 1 to 4 for each experimental animal group were orally administered 30 minutes before ingestion of ethanol. Then, 200 μl of 60% ethanol was orally administered. After 2 hours of ingestion of ethanol, a 5:1 mixture of anesthetics (zoletil, Rumpun) was diluted 1/7 and intraperitoneally injected at 6 mg/kg B.W. Whole blood was centrifuged (4°C, 10,000 rpm, 15 minutes) to separate serum, and stored at -80°C before use. Blood acetaldehyde was measured with an ELISA kit (Acetaldehyde kit, Roche, Switzerland) using the isolated serum. Blood ethanol was measured with an ELISA kit (Ethanol kit, Roche, Switzerland) using the isolated serum, and CYP2E1 was also measured using the isolated serum with an ELISA kit (Mouse Cytochrome p450 2E1 (CYP2E1) ELISA kit, China).

1-4. Comparison of Ethanol Concentrations in Plasma

The measurement results of plasma ethanol concentrations for each group are shown in Table 3 below.

group Plasma Ethanol Concentration (g/l) Ethanol + control (saline) 4.80 Ethanol + Example 1 0.45 Ethanol + Comparative Example 1 2.38 Ethanol + Comparative Example 2 2.15 Ethanol + Comparative Example 3 1.89 Ethanol + Comparative Example 4 1.47*

As shown in Table 3, the Example 1 group administered with the beverage composition for relieving hangover and improving liver function of the present invention showed the most significantly lowered ethanol concentration. And, Comparative Example 4 different from the mixing ratio of the beverage composition of the present invention was confirmed to have less ethanol decomposition effect than Example 1.

On the other hand, Comparative Examples 1 to 3, in which some components were not included, showed that the concentration of ethanol in the blood was higher than that of Examples 1 and 4, indicating that the ethanol decomposition effect was significantly lowered.

1-5. Comparison of CYP2E1 Concentrations in Plasma

The measurement results of CYP2E1 concentrations in plasma for each group are shown in Table 4 below.

group CYP2E1 concentration in plasma (pg/ml) Ethanol + control (saline) 50.0 Ethanol + Example 1 17.9* Ethanol + Comparative Example 1 25.0 Ethanol + Comparative Example 2 20.7 Ethanol + Comparative Example 3 19.8 Ethanol + Comparative Example 4 19.3*

As shown in Table 4, it was confirmed that the CYP2E1 concentration in the Example 1 group administered with the beverage composition for relieving a hangover and improving liver function of the present invention was significantly lower than that of other groups. And it was confirmed that the group of Comparative Example 4 showed a concentration lower than that of Comparative Examples 1 to 3 but higher than that of Example 1. Comparative Examples 1 to 3 in which some components were not included were confirmed to have higher plasma CYP2E1 concentrations than Examples 1 and 4.

Therefore, it was found that the beverage composition of the present invention inhibits CYP2E1 expression, which is related to the induction of alcoholic liver damage.

Experimental Example 2: Human Subject Simplified Clinical Trial

2-1. Determination of Respiratory Ethanol Concentration

Six males aged 20 to 50 years old were each ingested 6ml/kg/bw of commercially available soju (Chamisul) with an ethanol concentration of 17 degrees within 5 minutes, and each of the control group (saline), Examples 1 and Comparative Examples 1 to 4 300mg/kg was taken twice 30 minutes before/after ethanol intake, and the alcohol concentration was measured 1, 2, and 3 hours after ingestion of ethanol with a breathalyzer (Breathlizer, Sentech Korea, AL9000), and the result was measured Table 5 shows.

group Measurement time (after ethanol intake) 1 hours(%) 2 hours(%) 3 hours(%) Ethanol + control (saline) 0.084±0.03 0.065±0.03 0.054±0.02 Ethanol + Example 1 0.050±0.01 0.043±0.02 0.031±0.01 Ethanol + Comparative Example 1 0.079±0.03 0.068±0.02 0.050±0.01 Ethanol + Comparative Example 2 0.073±0.03 0.057±0.02 0.043±0.01 Ethanol + Comparative Example 3 0.065±0.03 0.050±0.02 0.039±0.01 Ethanol + Comparative Example 4 0.059±0.01 0.048±0.02 0.037±0.01

As shown in Table 5, it was confirmed that the respiratory alcohol concentration in Example 1 group administered with the beverage composition for relieving hangover and improving liver function of the present invention was significantly lower than that of other groups. Through this, it was found that the beverage composition of the present invention has excellent hangover relieving ability and has the effect of excellently reducing the respiratory alcohol concentration.

2-2. Sensory evaluation of hangover relief effect

In order to confirm whether the composition of the present invention actually relieves a hangover the day after drinking, the composition of the present invention was taken after drinking.

For each group, 10 healthy adult men and women over 20 years of age were tested. For the experiment, the subjects were instructed to drink 300 ml of soju over 60 minutes with the same amount of meal, immediately after drinking, as a control group (saline), Each of the compositions of Example 1 and Comparative Examples 1 to 4 was ingested by 4 g. The items for the hangover removal effect, digestive effect, and headache degree confirmed by these groups the next morning, 'No effect' as 1, and 'Highest effect' as 5, the range of 5 steps (1 to 5) was written and shown in Table 6 below.

group hangover removal effect degree of digestion degree of headache reduction Ethanol + control (saline) 1.0 2.4 2.8 Ethanol + Example 1 4.3 4.0 4.3 Ethanol + Comparative Example 1 2.7 2.9 3.0 Ethanol + Comparative Example 2 3.1 3.4 3.2 Ethanol + Comparative Example 3 3.9 3.7 3.8 Ethanol + Comparative Example 4 4.1 3.8 4.1

As shown in Table 6, it was confirmed that the beverage composition of the present invention had the highest hangover relieving effect.

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (4)

Including plant blend extracts of dermis, garlic root, goji berry and dandelion, milk thistle extract, calcium-chelate nano collagen peptide, turmeric extract, guarana extract, naringin extract and hesperidin extract, compared to the total weight of the composition
The plant mixed extract is 70 to 90 parts by weight,
The milk thistle extract is 1 to 10 parts by weight,
The calcium-chelate nano collagen peptide is 0.3 to 5 parts by weight,
The turmeric extract is 0.01 to 0.1 parts by weight,
The guarana extract is 0.1 to 0.5 parts by weight,
The naringin extract is 0.01 to 0.1 parts by weight,
The hesperidin extract is a beverage composition for relieving a hangover and improving liver function, characterized in that 0.1 to 0.5 parts by weight.
delete According to claim 1,
The composition further comprises one or more amino acids selected from the group consisting of taurine, histidine, arginine, lysine, glycine, threonine, glutamine, methionine and asparagine.
According to claim 1,
The composition is a beverage composition for relieving a hangover and improving liver function that further comprises calcium citrate and anhydrous citric acid.