KR102322276B1 - Beverage composition for preventing obesity and relieving edema - Google Patents

Abstract

The present invention relates to a beverage composition for preventing obesity and improving edema, and by including a natural product mixed extract, L-carnitine and calcium nano-collagen peptide as active ingredients, to prevent and improve various diseases caused by obesity and edema .

Description

Beverage composition for preventing obesity and relieving edema

The present invention relates to a beverage composition for preventing obesity and improving edema.

Obesity refers to an excessive accumulation of body fat due to an imbalance in energy intake and consumption, and the causes include a westernized diet, endocrine disorders, and genetic factors. Obesity itself is a health problem, but it is also a problem as a factor causing metabolic syndrome such as type 2 diabetes, high blood pressure, hyperlipidemia, and arteriosclerosis.

Recently, as the living standards of modern people have improved, the number of patients with obesity and metabolic diseases has rapidly increased around the world, which has become a social problem. According to the 2012 National Nutrition and Health Survey, the prevalence of obesity in Korea was 32.8%, which increased by 3.6% from 29.2% in 2001.

Improvement of eating habits and regular exercise are required for prevention and treatment of obesity. However, due to the characteristics of modern people, major efforts to control obesity are focused on anti-obesity drugs and health supplements.

On the other hand, edema (Edema) refers to a phenomenon in which the skin swells due to abnormal accumulation of body fluid in the interstitial tissue of the body. Edema requires an increase of at least 3 to 4 L in body fluid for symptoms to appear, so usually the body weight increases by 3 to 4 kg before edema appears.

70% of our body is water, 2/3 of which is inside the cell, the other 1/3 is outside the cell, 25% of the extracellular water is in the blood, and 75% is between the cells. It is present in the interstitial fluid. Water can move between the blood and the interstitium, and when the pressure inside the blood is high or the osmotic pressure inside the blood is low, the water in the blood moves to the interstitial space, which is the space between cells, causing edema.

Edema can be classified into generalized edema and localized edema according to the site of occurrence. In general, systemic edema is usually caused by heart disease, kidney disease, liver disease, and local edema is caused by a local circulatory abnormality.

Treatment of edema is divided into causal treatment and symptomatic treatment. In case the cause treatment fails, symptomatic treatment is performed. Edema appears in various forms from mild to severe, and the causes are many. should be approached with interest. For the treatment of edema, salt and water restriction and, if necessary, a diuretic to urinate well can be used to relieve and improve the edema.

Recently, a composition for enhancing renal function and improving edema, which is enhanced with arginine and citrulline, has been introduced. Arginine and citrulline amino acids are constituent amino acids of the urea cycle that decomposes ammonia, a toxic substance, into urea, a non-toxic substance in the liver. It is not possible to improve all the edema that occur in each part of the body.

As such, obesity and edema are diseases that require constant management in daily life, and thus, they often receive help from health supplements along with treatment of the cause, improvement of eating habits, and exercise.

Accordingly, interest in drugs with excellent anti-obesity effects while being safe for the prevention and treatment of obesity and alleviation and improvement of edema symptoms is increasing. have.

Accordingly, the inventors of the present invention developed a health functional food beverage composition for preventing obesity and improving edema containing a natural product mixture extract, L-carnitine and calcium nano-collagen peptide after studying for prevention of obesity and improvement of edema. We aim to help improve the quality of life and promote health of modern people.

Korea Patent Publication No. 10-1475138

The present invention contains a natural product mixture extract, L-carnitine, and calcium nano collagen peptide, thereby providing an excellent effect of inhibiting fat production, reducing body weight and body fat, and improving blood lipid levels, and promoting lymphatic circulation in the body to edema The purpose is to provide an effect that can quickly relieve or treat

In addition, an object of the present invention is to provide a beverage composition for preventing obesity and improving edema, which is safe for the human body by using a natural product as an active ingredient.

The present invention is (A) a natural product mixture extract; (B) L-carnitine; And (C) provides a beverage composition for preventing obesity and improving edema comprising calcium nano-collagen peptide as an active ingredient.

In addition, the present invention provides a beverage for preventing obesity and improving edema comprising the composition for beverages.

The present invention provides an excellent effect of inhibiting fat production, reducing body weight and body fat, and improving blood lipid levels by the synergistic effect of the combined use of a natural product mixed extract, L-carnitine and calcium nano collagen peptide, It promotes lymphatic circulation and provides an effect that can quickly relieve or treat edema.

In addition, the present invention has a safe effect on the human body by using a natural product as an active ingredient.

1 is a graph showing the accelerating effect of the composition of the present invention on the decomposition of triglycerides in adipocytes.
Figure 2 is a graph showing the edema improvement effect of the lower extremity of the composition of the present invention.

Hereinafter, the present invention will be described in more detail to help the understanding of the present invention. At this time, the terms or words used in the present specification and claims should not be construed as being limited to conventional or dictionary meanings, and the inventor appropriately defines the concept of the term in order to best describe his invention. Based on the principle that it can be done, it should be interpreted as meaning and concept consistent with the technical idea of the present invention.

The present invention relates to a composition for a beverage for preventing obesity and improving edema, and an object of the present invention is to prevent and improve various symptoms according to obesity and edema.

Specifically, the composition for beverages for preventing obesity and improving edema of the present invention is (A) a natural product mixture extract; (B) L-carnitine; and (C) calcium nano-collagen peptide as an active ingredient.

(A) natural product mixture extract of the present invention has the effect of preventing obesity and alleviating edema through promoting blood circulation, promoting water metabolism, diuretic action, detoxification, mental and physical stability and stress relieving action.

The natural product may include at least one selected from the group consisting of pumpkin, beet, kelp, broccoli, dermis, angelica, cheongung, jeoksodu, lotus leaf, nogeun, dolmenari, sukjihwang, jujube, poison ivy, bamboo leaf, and lotus meat, and specifically As such, it may include pumpkin, beet, kelp, broccoli, dermis, angelica, cheongung, jeoksodu, lotus leaf, nogeun, parsley, Sukjihwang, jujube, poison ivy, bamboo leaf, and lotus meat, and more specifically, pumpkin, beet, kelp. , broccoli, dermis, angelica, cheongung, jeoksodu, lotus leaf, nogeun, water parsley, sukjihwang, jujube, poison ivy, bamboo leaf, and lotus meat.

In addition, the natural product

1) at least one selected from the group consisting of pumpkin, beets, kelp, and broccoli;

2) at least one selected from the group consisting of dermis, angelica, and cheongung;

3) at least one selected from the group consisting of jeoksodu, lotus leaf, nogeun, and water parsley;

4) at least one selected from the group consisting of Sukjihwang, and jujube; and

5) It may include one or more selected from the group consisting of poison ivy, bamboo leaves, and lotus meat.

1) Pumpkin, beets, kelp, and broccoli are rich in potassium, and thus excreting sodium and waste products from the body, and promoting water metabolism to remove swelling.

In addition, the above 2) dermis, angelica, and cheongung can be used as one of the shinonhaepyo (辛溫解表) drugs. Shinonhaepyo refers to a method of treating wind chill syndrome using drugs that are hot, spicy, and spread outward. This ingredient can play a role in removing swelling by acting on early hydrocephalus (早期水腫症), which is relatively severe swelling in the upper body.

Specifically, the dermis refers to the mature pericarp of Citrus unshiu Markovich or a related plant of the rutaceae family. The dermis releases qi agglomeration, strengthens the function of the spleen, and has a diuretic effect. In addition, it is rich in vitamin C, which promotes metabolism, promotes blood circulation, and helps to recover from fatigue. Effect, capillary protection and anticancer action, cholesterol lowering effect.

In addition, angelica and cheongung have energy to warm the body, such as protecting qi and blood and removing eohyeol, which helps to relieve cold hands and feet, and promotes blood circulation to quickly relieve and improve edema.

3) Jeoksodu, lotus leaf, nogeun, and dolmenari have an anti-inflammatory action and help detoxify the kidneys or liver, thereby contributing to active water metabolism in the body.

Specifically, jeoksodu refers to dried seeds of red beans (Phaseolus angularis W. F. Wight), a legume plant. The taste is sweet and slightly sour, the nature is flat, and it acts on the heart and small intestine. It has the effect of urinating well, promoting blood circulation, removing pus and detoxifying. It is mainly used for beriberi, edema, jaundice, swelling, diabetes, etc. It is known to be good for preventing and treating beriberi, especially since it contains vitamins Bl and B2.

Nogeun, refers to the rhizome of the reed (Phragmites communis Trinius) of the Grape family, and is also called Nomogeun (蘆茅根). Nogeun is used for the symptoms of chest pain, discomfort, fever, dryness of the essence, and burning of the mouth by lowering lung heat. As pharmacological actions, diuretic action, antipyretic action, hepatoprotective action, and hematopoietic enhancement action have been reported, and it is mainly used for edema, jaundice, and cystitis.

As a nutritional component, dolmenari is rich in B vitamins, vitamins A and C, and minerals, so it has the effect of improving liver function. In addition, it contains potassium, which inhibits the action of sodium in the body, helps to expel water and wastes, and strengthens the function of the kidneys.

4) Sukjihwang and jujube have vitality and blood retention, and have energy to warm the body such as protecting qi and removing eohyeol, which helps to relieve cold hands and feet, and promotes blood circulation to quickly relieve and improve edema. have.

Specifically, Sukjihwang refers to steaming and drying the roots of Rehmanneh glutinosa Libosch. var. purpurea Mak. The taste is sweet and slightly bitter, and the nature is warm. It is the main medicinal product of samultang and is used for symptoms such as heat resistance, dry throat, and thirst, which are caused by weakness of the body among various chronic diseases. Since ancient times, it is effective in treating Heodam (虛痰), so it has been consumed as a tea.

The above 5) poison ivy, bamboo leaf, and lotus root helps to stabilize the mind and body, and has a calming effect to relieve stress, thereby helping to improve edema.

Specifically, the poisonous bow was given the name because it does not move in the wind, and it means the root of the earthworm (Aralia cordata var. continentalis; Aralia continentalis Kitagawa). It has analgesic, sedative and vasodilatory effects, and is effective in removing swelling.

Yeonjayuk is the seed of the lotus flower (Nelumbo nucifera Gaertner) of the water lily family, and refers to the dried medicinal herbs by removing the seed coat. It invigorates the mind and spirit and is widely used to relieve symptoms such as upper and lower fever caused by stress.

On the other hand, the natural product mixed extract of the present invention is characterized in that by extracting by mixing natural products in an appropriate ratio in consideration of the efficacy of each component, it is characterized in that it exhibits an optimal effect of preventing obesity and improving edema.

Specifically, when the natural product of the present invention includes all of pumpkin, beet, kelp, broccoli, dermis, angelica, cheongung, jeoksodu, lotus leaf, nogeun, parsley, sukjihwang, jujube, poison ivy, bamboo leaf, and lotus meat, pumpkin, beet , kelp, broccoli, dermis, angelfish, cheongung, jeoksodu, lotus leaf, nogeun, water parsley, sukshihwang, jujube, poison ivy, bamboo leaf, and lotus root 10:10:2:10:2:3:1.5:5:2:2 It may be mixed in a weight ratio of :10:2:2:2:2:2.

In addition, when the natural product includes one or more of the components of 1) to 5) above, each component of 1) to 5) is 8 to 10:1 to 2:4 to 6:1 to 2 It may be mixed in a weight ratio of :1 to 2, and specifically, the components may be pumpkin, dermis, red soybean, sukhumang sulfur, and poison ivy.

On the other hand, the natural product mixture extract can be extracted by vacuum high temperature extraction, hot water extraction, reflux extraction, hot water extraction, cold extraction, room temperature extraction, ultrasonic extraction or steam extraction method, but is not limited thereto.

Specifically, the natural product mixture extract of the present invention can be obtained by repeatedly extracting the mixed natural product once or several times with one or more solvents selected from the group consisting of water and C1 to C4 linear or branched alcohols, more specifically As such, it is preferable that the natural product mixture extract is repeatedly extracted several times for 5 hours or more with about 10 times the amount of water and/or 50% ethanol of the natural product mixture raw material.

In addition, the beverage composition for preventing obesity and improving edema of the present invention is characterized in that it contains (B) L-carnitine as an active ingredient.

L-carnitine is one of the vitamin B complexes containing the basic amino acids lysine, methionine, and NH ₄ ⁺ (ammonium ion). It increases the energy use of fatty acids by increasing the acetyl-CoA/CoA ratio in mitochondria. . The chemical formula is C ₇ H ₁₅ NO ₃ , which is known to be effective for dieting when consumed and exercised because it helps burn fat, and is also used as a diet supplement. It can be absorbed through animal proteins such as lamb or beef, which are rich in the basic amino acids lysine and methionine. However, in case of deficiency, there is a problem in the breakdown of fatty acids, so a smooth diet may not be achieved.

It helps in recovery after exercise, supplies oxygen to the muscles, improves stamina, reduces muscle pain, and is also known to help red blood cell production. The absorption rate in the body is known to show an absorption rate of 50 to 80% in the case of food and 20% or less in the case of a supplement.

In the present invention, L-carnitine serves to reduce the amount of triglycerides in the blood and reduce body weight. Therefore, since it is involved in the process of decomposition of body fat, it is possible to increase the effect of exercise by taking it at a time when body fat decomposition is active, such as during exercise.

In addition, along with calcium, it prevents osteoporosis, acts on muscle tissue to further strengthen muscle strength, and also exhibits an anti-aging effect.

In addition, the beverage composition for preventing obesity and improving edema of the present invention is characterized in that it contains (C) calcium nano-collagen peptide as an active ingredient.

The calcium nano-collagen peptide may be a nano-collagen peptide represented by the following formula (1).

[Formula 1]

(R represents a side chain that determines the type of amino acid, and M is at least one selected from the group consisting of Ca, Cu, Zn, Fe, Se, Cr, Mg, Mn and Co, specifically Ca.)

The calcium nano-collagen peptide of the present invention is a complex in which nano-unit collagen peptides and calcium, a mineral, are chelated, and can be used in food, medicine, quasi-drugs, cosmetics or feed, etc. In this case, it improves the preservation and stability of the product, and Alternatively, there is an effect of improving the absorption rate of calcium chelated to collagen peptides as well as peptides in the body.

The calcium nano-collagen peptide is prepared by 1) adding an enzyme to collagen to hydrolyze it to form a collagen peptide having a nano-unit size; and 2) adding calcium to the collagen peptide to form a calcium nano-collagen peptide chelate.

Collagen, also called hard protein or collagen, is widely distributed in multicellular animals such as invertebrates and vertebrates and is the most quantitatively found hard protein in the human body. It is mainly present in the cornea, bones and skin, and plays a very important role as a component of the dermis layer in the skin. It is known that the main function of collagen is the firmness of the skin, the resistance and bonding of tissues, and the support of cell adhesion.

Collagen can be largely divided into animal collagen such as cow skin and pig skin, vegetable collagen such as fruits, vegetables, seeds, nuts and mushrooms, and marine collagen such as seaweed, fish, fish scales, and fish skin, and production of calcium nano collagen peptide of the present invention Collagen used for is characterized in that at least one selected from the group consisting of animal collagen, vegetable collagen, and marine collagen.

Collagen has a triple helix structure in which three polypeptide chains are twisted, and the average molecular weight of collagen is 300,000 Da (Dalton, Dalton), which is high. In order to increase the absorption rate of collagen having a high molecular weight, the calcium nano-collagen peptide of the present invention is prepared by adding an enzyme to hydrolyze the collagen to decompose it into nano-unit collagen peptides.

The average molecular weight of the collagen peptide of the present invention is 200 Da to 600 Da, more specifically 300 Da to 400 Da, and the size is nanosized to 0.1 nm to 10 nm, more specifically 1 nm to 5 nm, Due to its small molecular weight and small size, it has the effect of being rapidly absorbed into the body or skin.

Here, the enzyme used in the collagen nano-forming step is a proteolytic enzyme, characterized in that it comprises at least one selected from the group consisting of collagenase, trypsin, papain, pepsin and alkalinease, and converting collagen into collagen peptides. The decomposing hydrolysis reaction is characterized in that it is carried out at 50 °C to 70 °C, more specifically, at 50 °C to 60 °C for 2 hours to 24 hours.

When the hydrolysis reaction occurs under the conditions of temperature and time in the above range, it is possible to obtain a collagen peptide having an appropriate molecular weight and size having a high absorption rate in the body.

After the collagen nano-ing step, 2) calcium nano-collagen peptide chelate can be prepared by adding calcium to the collagen peptide obtained through 1) step.

The chelate refers to a bond when a ligand having a plurality of coordination groups for coordinating a metal ion in one molecule is coordinated with a metal, or a complex formed by the bonding. In the present invention, the unshared electron pair that does not participate in the covalent bond of the amino group bonded to the alpha carbon of the amino acid forms a complete coordination bond with the mineral to form a calcium nano-collagen peptide chelate.

Calcium used in the present invention may be calcium carbonate, calcium hydroxide or calcium sulfate, and in this case, other types of minerals may be used instead of calcium, and the minerals include Cu, Zn, Fe, Se, Cr, Mg, Mn and It may correspond to one or more hydroxides or sulfur oxides selected from the group consisting of Co.

Calcium used is characterized in that 1 to 20 parts by weight based on 100 parts by weight of the collagen peptide, and within the above range, calcium nano-collagen peptides can be obtained with high purity without unreacted calcium.

In the present invention, the calcium nano-collagen peptide serves as an ion balance in the body and serves as a high-quality protein source to prevent the yo-yo phenomenon.

In the calcium nano-collagen peptide, collagen is hydrolyzed into nano-sized peptides, so the bioabsorption rate and utilization rate can be maximized compared to conventional collagen. When the protein is insufficient, the muscle is weakened and the possibility of edema is increased, so by including the nano-collagen peptide, the effect of alleviating and improving edema can also be obtained.

In addition, it increases the absorption rate of calcium in the body and helps to maximize the bioavailability. When used in food, calcium helps the formation of bones and teeth by supplying calcium in the body, strengthens alveolar bone to keep gums healthy, maintains nerve and muscle functions, reduces the risk of osteoporosis, and balances the potential difference of cell membranes It can help with vascular disease and heart disease.

In addition, the calcium nano-collagen peptide of the present invention can maximize the amount of calcium reaching the small intestine by tightly binding the collagen peptide and calcium through coordination covalent bonding, thereby preventing anion binding and competitive absorption. Furthermore, since absorption is possible without being ionized by enzymes, it can be absorbed not only in the upper part of the small intestine but also in the entire small intestine. In addition, fat solubility can be secured through binding with collagen peptide, and thus can be absorbed from the entire cell membrane, thereby maximizing the amount of calcium absorbed into cells from the small intestine. Furthermore, by minimizing the effect of vitamin D, calcium absorption can be made stably.

Furthermore, the calcium nano-collagen peptide of the present invention is effective in suppressing aging due to ultraviolet rays and improving wrinkles, and can provide an effect of maintaining moisture by increasing the moisture content of the skin when used as a cosmetic.

Meanwhile, the composition of the present invention may further include at least one selected from the group consisting of vitamins B1 (thiamine), B2 (riboflavin), B3 (nicotinamide) and B6 (pyridoxine).

The vitamin B complex is water-soluble vitamins that play an important role in cellular metabolism. Specifically, it strengthens immune system and nervous system functions, reduces the risk of pancreatic cancer, maintains skin color and muscle health, and promotes metabolism.

In addition, the present invention may further include lactose, xylitol, sucralose, cyclodextrin, or erythritol as a sweetener.

In addition, the composition of the present invention is characterized in that it contains the active ingredients (A), (B) and (C) in an appropriate weight ratio, and specifically, the composition of the present invention contains the active ingredients (A), (B) and (C) can be appropriately adjusted according to the symptoms of the disease, the degree of progression of the symptoms, the condition of the patient, etc., but a weight ratio of 100 to 150:1 to 10:1 to 50, more specifically 130 to 150:1 to 5:1 It is most preferably mixed in a weight ratio of 20, more specifically 130 to 140:1 to 5:1 to 10.

When each component is included within the above range, it is possible to obtain a remarkably excellent effect in preventing obesity and improving edema. The above weight ratio is a value based on the dry amount after removing the solvent.

The beverage composition for preventing obesity and improving edema of the present invention may include other active ingredients and/or food pharmaceutically acceptable food supplement additives in addition to the active ingredients, and suitable carriers, excipients and It may further include a diluent.

That is, nutrients, vitamins, electrolytes, flavoring agents, coloring agents, thickeners, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonated beverages It may further contain a carbonation agent and the like. The above components may be added independently or in combination.

In addition, the present invention provides a beverage for preventing obesity and improving edema comprising the composition for beverages.

The beverage composition of the present invention may be included in a ratio of 0.001 g to 15 g, specifically 0.01 g to 10 g, more specifically 1 g to 10 g, based on 20 ml of the total volume of the beverage, and the remainder includes purified water can do.

As an ingestion method, the beverage composition can be consumed as it is or diluted to a low concentration to make a beverage and drink it instead of water or drink it as a brewed tea in warm water.

Recently, a tea that prevents obesity by decomposing body fat and relieves or improves edema to remove swelling, aka puffiness tea, is popular. When the present invention is used as a tea for swelling, it may be prepared in a liquid form for direct drinking or diluted in water for ingestion.

Specifically, when preparing pouring tea with the beverage composition of the present invention, 8 g of the beverage composition of the present invention may be included on the basis of 20 ml per package, and the recommended intake may be 2 times a day, 1 package per time. .

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

Preparation 1-1

10 times water was added to 33.75 g of natural product having the composition of Table 1 below, and reflux extraction was repeated twice at about 50° C. for about 5 hours. The obtained extract was filtered and concentrated under reduced pressure at 50° C. or less to obtain an extraction yield of 20% to prepare 6.75 g of a natural product mixture extract.

ingredient Weight(g) Pumpkin (dried) 5.0 beet (raw) 5.0 Kelp (dried) 1.0 Broccoli (raw) 5.0 dermis 1.0 donkey 1.5 celestial palace 0.75 small head 2.5 lotus leaf 1.0 Nogeun (reed) 1.0 dolphins 5.0 Sook Hwang 1.0 Jujube 1.0 solitary 1.0 bamboo leaf 1.0 soft meat 1.0 total 33.75 Extraction production yield (20%) 6.75

Preparation 1-2

By extracting 33.75 g of a natural product having the composition of Table 2 in the same manner as in Preparation Example 1-1, 6.75 g of a natural product mixed extract was prepared.

ingredient Weight(g) Pumpkin (dried) 16.0 dermis 3.25 small head 9.5 Sook Hwang 2.0 solitary 3.0 total 33.75 Extraction production yield (20%) 6.75

Preparation 2

The marine collagen of the fish skin and jellyfish was washed with purified water to remove impurities, and 4 liters of purified water was added to 1 kg of raw material and crushed to 50 μm with a chopper.

Then, the raw material deodorized by passing ozone is put into a 0.5% solution of citric acid, and 0.5 w% of pepsin protease is added and hydrolyzed at 55° C. for 24 hours to obtain a molecular weight of 300 Da to 400 Da, size 1 nm to 5 nm. Collagen peptides were obtained.

For the preparation of calcium nano-collagen peptide, calcium carbonate was added so that the calcium content was 1 to 20 parts by weight relative to 100 weight of the collagen peptide, and enzymatic hydrolysis was performed at 55 ° C. and dried to prepare a chelate complex powder of calcium nano-collagen peptide.

Example 1

By adding 0.0025 g each of vitamins B1, B2, B3 and B6 to 6.75 g of the natural product mixture extract of Preparation Example 1-1, 0.05 g of L-carnitine, and 0.25 g of the calcium nano-collagen peptide of Preparation Example 2, the obesity prevention and 7.06 g of a beverage composition for improving edema was prepared.

Example 2

By adding 0.0025 g each of vitamins B1, B2, B3 and B6 to 6.75 g of the natural product mixture extract of Preparation Example 1-2, 0.05 g of L-carnitine, and 0.25 g of the calcium nano-collagen peptide of Preparation Example 2, the obesity prevention and 7.06 g of a beverage composition for improving edema was prepared.

Comparative Example 1

A beverage composition was prepared in the same manner as in Example 1, except that in Example 1, the natural product mixture extract of Preparation Example 1-1 was not mixed.

Comparative Example 2

A beverage composition was prepared in the same manner as in Example 1, except that L-carnitine was not mixed in Example 1.

Comparative Example 3

A beverage composition was prepared in the same manner as in Example 1, except that the calcium nano-collagen peptide of Preparation Example 2 was not mixed in Example 1.

Experimental Example 1: Weight gain inhibitory effect

Six-week-old male SD rats were purchased from Orient (Seongnam, Korea) and 10 rats per group were prepared and used after acclimatization for 1 week. After acclimatization for one week, each animal was grouped as follows. Each group was separated into individual cages and managed according to a 12-hour day (7–17:00) and night cycle.

- Normal: normal diet

- High-Fat: high-fat diet

- Example 1: High-fat diet group + Example 1 administration group (50 mg/kg/day)

- Example 2: High fat diet group + Example 2 administration group (50 mg/kg/day)

- Comparative Example 1: High-fat diet group + Comparative Example 1 administration group (50 mg/kg/day)

- Comparative Example 2: High fat diet group + Comparative Example 2 administration group (50 mg/kg/day)

- Comparative Example 3: High-fat diet group + Comparative Example 3 administration group (50 mg/kg/day)

To each experimental group, the beverage composition was orally administered once a day for 8 weeks at a fixed time (10 am), and the same amount of water was administered to 10 animals in the high-fat diet group as a control group. At this time, as shown in Table 3, the total calories of the high-fat diet and the normal diet are the same as 4,057 kcal, but in the high-fat diet, fat accounts for 60% of the total calories, and in the normal diet, fat accounts for 10%.

ingredient High-fat diet ¹⁾ (g/kg) Normal diet ²⁾ (g/kg) Casein 200 200 corn powder 0 315 maltodextrin 125 35 sucrose 68.8 350 cellulose 50 50 soybean oil 25 25 Lard 245 20 mineral mix 10 10 calcium phosphate 13 13 calcium carbonate 5.5 5.5 potassium citrate 16.5 16.5 vitamin mix 10 10

1) syntheric diet D12494 (Research Diets, Inc)2) syntheric diet D12450B (Research Diets, Inc)

The body weight was measured once a week (11 am) during the experiment period, and the final body weight of the experimental group and the control group was measured 8 weeks after the start of administration to calculate the change in body weight, and the results are shown in Table 4 below.

Weight (g) before the start of the experiment Weight (g) after the end of the experiment Weight change (%) Normal (n=10) 18.76±0.84 26.3±1.78 40% High-Fat (n=10) 20.28±0.62 34.15±2.16 68% Example 1 (n=10) 19.10±0.54 27.35±1.66 43% Example 2 (n=10) 19.80±0.50 29.30±1.52 48% Comparative Example 1 (n=10) 20.60±1.05 34.05±3.3 65% Comparative Example 2 (n=10) 20.25±0.83 34.35±3.41 69% Comparative Example 3 (n=10) 19.96±0.89 33.85±2.5 69%

As shown in Table 4, the group ingesting Comparative Examples 1 to 3 did not confirm a specific weight loss effect compared to the high-fat diet group. On the other hand, in the group ingesting Example 1, the weight change was about 43% after 8 weeks, confirming that the weight gain inhibitory effect was superior to that in the high-fat diet group, and then, the group taking Example 2 showed a weight change of about 48%. It was confirmed that the increase inhibitory effect was excellent. (p<0.05)

Therefore, it was confirmed that when the natural product mixture extract according to the present invention, L-carnitine and calcium nano-collagen peptide were used in combination, there was a statistically significant effect of inhibiting weight gain and preventing obesity.

Experimental Example 2: Confirmation of the effect of promoting the decomposition of triglycerides in adipocytes

After separating the epididymal adipose tissue from 6-week-old male SD rats, it was chopped with scissors, and 0.1% collagenase (in DMEM without phenol red) was added thereto. Thereafter, the cells were incubated at 37° C. for 2 hours and then filtered to obtain adipocytes.

Colorless DMEM (Dulbeco's modified eagles medium) containing 0.5% bovine serum albumin (BAS) without fatty acids was added to adipocytes and then taken and used for each experiment. Quantification of glycerol was performed by a color reaction method using a GPO-trinder kit purchased from Sigma, USA (St. Louis, Mo, U.S.A), and absorbance was measured at 540 nm using an ELISA reader.

For the control group, only a medium to which no test substance or comparative substance was added was used, and for the experimental group, 200 ppm of each of the compositions of Examples 1 and 2 and Comparative Examples 1 to 3 was added. Since fat is decomposed into fatty acids and glycerol when hydrolyzed, the degree of lipolysis was determined by measuring the concentration of glycerol liberated from adipocytes into the culture medium. and the results are shown in FIG. 1 .

As shown in FIG. 1 , the fatty acid decomposition effect showed significantly higher decomposition results in Examples 1 and 2 than the control and Comparative Examples 1 to 3. Therefore, it was confirmed that when the natural product mixed extract according to the present invention, L-carnitine, and calcium nano collagen peptide were used in combination, there was a statistically significant effect on decomposing fatty acids and preventing obesity.

Experimental Example 3: Confirmation of the effect of improving lower extremity edema

6-week-old male SD rats were purchased from Orient (Seongnam, Korea) and used after acclimatization for 1 week. After acclimatization for one week, each animal was grouped as follows.

- Normal: Normal control

- Edema: Excellent for lower extremity edema

- Example 1: edema inducing group + Example 1 administration group (50 mg/kg/day)

- Example 2: edema inducing group + Example 2 administration group (50 mg/kg/day)

- Comparative Example 1: Leg edema inducing group + Comparative Example 1 administration group (50 mg/kg/day)

- Comparative Example 2: Leg edema inducing group + Comparative Example 2 administration group (50 mg/kg/day)

- Comparative Example 3: Leg edema inducing group + Comparative Example 3 administration group (50 mg/kg/day)

For each group, the compositions prepared in Examples 1 and 2 and Comparative Examples 1 to 3 were orally administered once every morning from 3 days before the induction of leg edema in each group, and leg edema was induced in the afternoon after 4 days of pretreatment. For oral administration, the compositions prepared in Examples 1 and 2 and Comparative Examples 1 to 3 were suspended and administered in 0.5% sodium carboxymethyl cellulose (CMC), and 0.5% in the normal control group and the leg edema-inducing group Only sodium carboxymethylcellulose was orally administered in the same amount.

Induction of lower extremity edema was induced by administering 0.1 ml of formalin in saline containing 2.5% formalin to the sole of the right hind leg, and only physiological saline was injected in the same amount in the normal group.

An increase in paw volume due to edema immediately before and 1 hour after administration of formalin was measured using a phethysmometer (Ugo Basile, Milan, Italy). The edema index was calculated by Equation 1 below.

[Equation 1]

Edema value (%) = {(Hourly foot size - first foot size)/first foot size} x 100

As shown in FIG. 2, as a result of confirming the edema state before and 1 hour after induction of edema in each group, there was a slight increase in the size of the lower extremities by injection of sterile physiological saline in the normal group, but it was mild, and formalin was administered to induce edema in the lower extremities. In the case of the group, the size was significantly increased by about 20%, but it was confirmed that the change in size due to the edema of the lower extremities was significantly inhibited by the administration of the compositions of Examples 1 and 2.

Based on the above results, it was confirmed that there was statistically an edema alleviation and improvement effect in the case of using the natural product mixed extract, L-carnitine and calcium nano collagen peptide according to the present invention in combination.

The description of the present invention described above is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (7)

(A) natural product mixture extract;
(B) L-carnitine; and
(C) containing calcium nano-collagen peptide as an active ingredient,
The natural products are pumpkin, beet, kelp, broccoli, dermis, angelica, cheongung, jeoksodu, lotus leaf, nogeun, dolphins, sukjihwang, jujube, poison ivy, bamboo leaf, and lotus leaf 10:10:2:10:2:3:1.5 :5:2:2:10:2:2:2:2: Beverage composition for preventing obesity and improving edema, characterized in that it is mixed in a weight ratio of 2.
delete delete delete delete According to claim 1,
The composition is a beverage composition for preventing obesity and improving edema, characterized in that (A), (B) and (C) are mixed in a weight ratio of 100 to 150:1 to 10:1 to 50.
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