KR102139328B1 - Cosmetic composition for skin cooling or improving skin redness with the extract of Eucommia Ulmoides bark - Google Patents

Abstract

The present invention is a cosmetic composition made of a material derived from a natural product, specifically, for reducing redness or increasing skin temperature due to externally exposed heat by using the genus biceps bark extract and geniposide and acubin contained in the cesium bark extract. Provided is a cosmetic composition for suppressing skin cooling and a method for manufacturing the same.

Description

Cosmetic composition for skin cooling or improving skin redness with the extract of Eucommia Ulmoides bark}

The present invention relates to a cosmetic composition for skin cooling or skin flushing relief containing an extract of bark beetle.

The skin covers the surface of the human body with an area that occupies about 16% of the human body, and is in direct contact with the external environment, which is closely related to external factors such as temperature and humidity. The optimal body temperature of the body is 36.5℃, and the skin is 31℃, which is 5~6℃, and the temperature is 31℃. If the temperature is too high or too low, skin health may deteriorate.

Recently, as the Earth has renewed its highest temperature since meteorological observations, it has been analyzed that high temperatures have continued throughout the world, including in Korea, and that even if current climate policies are implemented, it will lead to warming above 3℃. As the temperature of the earth rises, the temperature of the skin also rises due to external environments such as ultraviolet rays and infrared rays, thereby accelerating skin aging. Therefore, it is necessary to keep the proper skin temperature from the skin temperature caused by external stimuli.

The increase in skin and body temperature is a complex reaction involving both the peripheral immune system and the brain, and in the normal thermal pathway, body temperature rises with the production of Prostaglandin E2 (PGE2). PGE2 is a major mediator of fever. It binds to the receptors of thermoregulatory neurons located in the anterior hypothalamus and exhibits a fever action. Skin redness and swelling due to increased blood flow to the infected tissue through vasodilation and increased microvascular permeability by PGE2 This will happen. In this regard, in the experiment using a 3D artificial skin model, the study of increasing the production of PGE2 by thermal stimulation and the expression of PGE synthase (PGES) and the expression of heat shock protein (HSP)-70 induced therein were also studied. There is a bar.

However, research using natural materials for controlling skin heat in relation to PGE2 is insignificant. Accordingly, the present invention is to provide a cosmetic composition for alleviating skin flushing or cooling the skin by controlling the heat of the skin due to external stimulation by containing a natural plant extract.

Republic of Korea Patent Registration No. 10-1860426 (Registration Date: 2018.05.16) relates to a herbal medicine composition for skin cooling using a yellow-white extract and a manufacturing method thereof and a skin improvement product using the same, and prevents deterioration of skin and skin, and occurs Disclosed is an effect of lowering the skin heat and skin heat, and improving the prevention and treatment of itchiness, eczema, odor, swelling, and rash.

An object of the present invention is to discover and extract an extract of a natural product having excellent skin flushing effect and skin cooling effect caused by heat by controlling skin heat caused by external stimulation, and to develop and provide a cosmetic composition containing it as an active ingredient. .

The present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains an extract of Eucommia ulmoides bark.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains an extract of Eucommia ulmoides bark.

On the other hand, in the present invention, the bark tree bark extract, preferably may be to inhibit the expression of PGE2 synthase protein.

On the other hand, in the present invention, the bark tree bark extract, preferably may be contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.

In addition, the present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains Geniposide as an active ingredient.

In addition, the present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains Aucubin as an active ingredient.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains a geniposide (Geniposide) as an active ingredient.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains aucubin (Aucubin) as an active ingredient.

On the other hand, in the present invention, the geniposide (Geniposide) or Aucubin (Aucubin), may preferably be to inhibit the expression of PGE2 synthase protein.

On the other hand, in the present invention, the geniposide (Geniposide) or Aucubin (Aucubin), may be preferably contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.

On the other hand, in the present invention, the cosmetic composition, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizer, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, It may be one or more formulations selected from the group consisting of nutrient essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion or body cleanser.

The present invention provides a cosmetic composition for skin cooling and a method for manufacturing the skin cooling to suppress redness caused by externally exposed heat and to increase the skin temperature by using the extracts of bark bark bark, and the genoside, acubin contained in the bark bark bark extract do.

Anti-inflammatory effect by inhibiting the expression of PGE2 synthetase protein increased by heat killing effect, heat protection efficacy and heat treatment by using the genus biside, acubin contained in the cedar bark extract of the present invention and the cedar bark extract Can represent

1 is a graph showing the results of analysis of Geniposide using high performance liquid chromatography (HLPC).
Figure 2 is a graph of the results of the analysis of the content of Geniposide (Geniposide) in the extract of bark beetle bark purity analyzed using high performance liquid chromatography (HLPC).
3 is a graph of the results of Aucubin analysis using high performance liquid chromatography (HLPC).
Figure 4 is a graph of the analysis results of the content of Aucubin (Aucubin) in the extract of the cedar bark tree by purity analysis using high performance liquid chromatography (HLPC).
5 is a graph showing the results of the HaCaT cytotoxicity test through MTT assay for the control group and the cedar bark extract and the active ingredients Geniposide and Aucubin.
6 is a photograph comparing the degree of staining according to JC-1 staining for the control group and the extract of the cedar bark and the active ingredients Geniposide and Aucubin.
7 is a graph comparing the mRNA expression level of the related gene TRPV4 after irradiation with UVB for the control group and the cedar bark extract and the active ingredients Geniposide and Aucubin.
8 is a graph comparing the expression level of PGE2 synthetase protein for the control group and the cedar bark extract and the active ingredients Geniposide and Aucubin.
Figure 9 shows a comparison graph of the skin surface temperature according to before and after the use of each product for the gel group containing the extract of the bark tree bark (Example 2) and the group using the gel of the bark tree bark extract (comparative example). .
FIG. 10 shows a comparison graph of skin blood flow according to before and after the use of each product for the group using the gel containing the cedar bark extract (Example 2) and the product using the gel containing the cedar bark extract (comparative example).

Eucommia ulmoides ( Eucommia ulmoides ) is a deciduous tree belonging to the family Nephi , and its leaves are oval-shaped, and the fruit is cut into a flat long circle to produce a rubbery, viscous thread, and the dried bark is used as a medicinal herb. The bark of the wormwood tree is called wormworm, and the young leaves are cotton, and is used for medicinal purposes.

The present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains an extract of Eucommia ulmoides bark.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains an extract of Eucommia ulmoides bark.

In the cosmetic composition of the present invention, the cedar bark extract can use various extraction methods commonly used in the art. For example, purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane solvent, respectively, or a mixture of two or more of them may be extracted using a mixed solvent as an extraction solvent.

In the present invention, after drying and crushing the bark of the cedar tree, an extract of the cedar tree bark is obtained using ethanol, filtered with a filter and concentrated, and then the cedar tree bark extract prepared by adding butylene glycol Used.

In the cosmetic composition of the present invention, the bark bark extract may be one that inhibits the expression of PGE2 synthase protein.

Prostaglandin E2 (PGE2) is a type of bioactive compound synthesized in vivo, and acts as a main medium for fever, binding to the receptors of thermoregulatory neurons in the anterior hypothalamus and exerting heat, thereby acting to increase body temperature. Do it. In addition, through the expansion of blood vessels and microvascular permeability by PGE2, blood flow to the infected tissue increases, resulting in skin flushing and edema.

In the cosmetic composition of the present invention, the bark tree bark extract is preferably contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.

In addition, the present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains Geniposide as an active ingredient.

Geniposide is a component of iridoid glycosides and is present in about 40 species of natural plants, and is known to exhibit pharmacological effects such as anti-inflammatory, antioxidant, and immunomodulatory properties.

In addition, the present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains Aucubin as an active ingredient.

Aucubin is a component found in natural plants as a component of iridoid glycosides, and is known to exhibit pharmacological effects in protecting liver damage, enhancing immunity, and treating gout.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains a geniposide (Geniposide) as an active ingredient.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains aucubin (Aucubin) as an active ingredient.

In the cosmetic composition of the present invention, the geniposide or Aucubin may inhibit the expression of PGE2 synthase protein.

In the cosmetic composition of the present invention, the geniposide (Geniposide) or Aucubin (Aucubin) is preferably contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.

In the cosmetic composition of the present invention, the cosmetic composition, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence , Nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion or may be one or more formulations selected from the group consisting of a body cleanser.

In addition, the cosmetic composition of the present invention can be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may be varied according to a user's skin condition or taste.

Hereinafter, the present invention will be described in more detail in the following Examples and Experimental Examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes all modifications of equivalent technical ideas.

[Example 1: Preparation of cedar bark extract]

After drying and grinding the cedar bark, the cedar tree is 50% ethanol in 50% ethanol = 1:5, preferably 1:8 by weight ratio of 30 to 80°C for 1 to 5 days, preferably 50 It was stirred for 1 day at ℃ to obtain an extract of bark beetle. Next, the cedar bark extract was filtered with a filter having a mesh size of 0.1 to 10 um, preferably 1 um to 0.3 um. The filtered bark tree bark extract was concentrated at a temperature of approximately 40-70°C, preferably 60°C, to prepare a concentrate. Butylene glycol was added to the prepared concentrate to add 10 to 50% by weight, preferably 30% by weight, to prepare a cedar bark extract.

[Experimental Example 1: Analysis of the content of the bark extract of the cedar tree]

In this experimental example, the extracts of Example 1 were analyzed for the contents of Table 1 (Zeniposide) and Table 2 (Aucubin), which are active ingredients of the extract of bark beetle. Purity analysis was performed using high performance liquid chromatography (HPLC) under the described conditions, and the results are shown in Table 3 and FIGS. 1, 2, 3 and 4 below.

Purity analysis conditions using high performance liquid chromatography (HLPC) for analyzing the content of Geniposide Column Capcell Pak C18 4.6 X 250mm
(Shiseido HPLC Column) Column temperature 35℃ Flow rate 1.0mL/min Detector UV 239nm Analysis time 30 min Injection Volume 20 μl Mobile phase Acetonitrile: Deionize Water= 15: 85

Purity analysis conditions using high performance liquid chromatography (HLPC) for the analysis of the content of Aucubin Column Capcell Pak C18 4.6 X 250mm
(Shiseido HPLC Column) Column temperature 35℃ Flow rate 0.5mL/min Detector UV 210nm Analysis time 30 min Injection Volume 20 μl Mobile phase Acetonitrile: Deionize Water= 5: 95

Purity analysis results using high performance liquid chromatography (HLPC) Indicator Duchung tree extract (ppm) Geniposide 250 ~ 350 Aucubin 700 ~ 1,200

[Experimental Example 2: Cytotoxicity evaluation of bark tree bark extract (MTT assay)]

In this experimental example, the cytotoxicity of the extract of the cedar bark of Example 1 and its active components, Geniposide and Aucubin, were measured.

After inoculating keratinocytes (HaCaT) in a 24-well plate and incubating for 24 hours, the medium of the cultured cells was replaced with serum free medium, and the samples were processed for each concentration to incubate for 24 hours. After the reaction was performed by replacing the cultured cells with a medium diluted with 10 times of MTT solution of 2.5 mg/ml, the supernatant was removed, and 1 ml of DMSO was added to dissolve the resulting MTT-formazan crystals and absorbed at 570 nm with an ELISA Reader. Was measured. Cytotoxicity is expressed as a percentage of absorbance compared to the control, which is shown in Table 4 below.

As shown in Table 4 and Figure 5 below, as a result of performing a HaCaT cytotoxicity test through MTT assay to determine the sample concentration for the evaluation of the efficacy of the cedar bark extract and indicator components geniposide and aucuvin, cedar bark extract Silver was toxic at a concentration of 250 ug/ml, and Geniposide and Aucubin were not toxic.

Sample Conc.
(Μg/ml) Cell viability (%) Stdev. Control 100.0 0.8 Cedar bark
extract One 101.0 2.8 10 100.4 0.8 25 97.8 2.7 50 105.1 1.9 100 102.8 1.2 250 85.2 1.3 500 61.9 1.2 Geniposide 0.1 101.6 1.3 One 104.6 4.1 2.5 101.9 0.8 5 98.4 0.8 10 100.8 0.9 25 97.5 2.2 50 101.2 1.7 100 96.3 1.0 Aucubin 0.1 100.4 1.7 One 103.4 4.4 2.5 102.2 3.1 5 101.7 4.7 10 100.0 2.9 25 105.9 2.4 50 105.2 4.6 100 96.2 2.1

[Experimental Example 3: Confirmation of the light-protecting efficacy of the cedar bark extract]

In this experimental example, the light-protecting efficacy of the extract of the cedar bark of Example 1 and its active components, Jenny Poside and Aucubin, were measured.

To verify the mitochondrial cell membrane potential change, keratinocytes (Human keratinocytes) were dispensed into 35 mm plates, stabilized for 24 hours, and then replaced with serum-free medium and treated with samples. After 1 hour, after washing with HBSS (Hank's Balanced Salt solution) buffer, fresh HBSS was added and UVB 50 mJ/cm 2 was irradiated. Then, after replacing the medium with a serum-free medium, the sample was processed and incubated for 24 hours in a 37°C, 5% CO2 incubator. After removing the medium, 1 μg/ml of JC-1 solution was treated and reacted for 2 hours without light in a 37°C, 5% CO2 incubator to remove the medium and observed under a fluorescence microscope.

As a result of confirming the degree of staining of the extract of the bark tree bark and its active components, geniposide and aucubin, as compared to the control UVB treatment group, as shown in FIG. 6, in Example 1, the effect of inhibiting apoptosis at a concentration of 100 ug/ml was obtained. appear. The geniposide showed efficacy in the entire range of the treated concentration, and it was confirmed that the red fluorescence and the number of cells significantly increased compared to the UVB irradiation group at a particularly low concentration. Aucuvin showed efficacy at a concentration of 10 ug/ml or less. Therefore, it is considered that the extract of bark beetle and indicator components geniposide and aucuvin have high efficacy in inhibiting apoptosis by UVB.

[Experimental Example 4: Confirmation of heat protection efficacy of bark extract of cephalobacteria]

In this experimental example, the effect of heat protection of the skin was measured through the extract of the bark of the cedar tree of Example 1 and its active ingredients, Jenny Poside and Aucubin.

After inoculating HEKn cells in 6 well multidish and incubating for 24 hours, the medium of the cultured cells was replaced with HBSS and UVB 100 mJ/cm 2 was investigated. After that, the cells were replaced with serum-free medium, and the samples were processed for each concentration to incubate for 24 hours. Cells were collected with TRIzol and RNA was isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA was synthesized with 2 μg of RNA to perform real-time PCR. This is shown in Tables 5 and 6 below.

As a result of the Real-Time PCR experiment, as shown in FIG. 7, the cedar bark extract reduced the expression of TRPV4 increased by UVB concentration-dependently, and decreased the expression of TNFV4 and geniposide used as active ingredients. Therefore, it is believed that the extracts of bark of the cedar tree, and the indicator components of Zeniposide and Aucubin, have a thermal protection effect that protects the skin from being damaged by ultraviolet rays.

Sample Conc. (Μg/ml) TRPV4 Expression rate
(% UVB, Actin correction) Stdev. Control 74.4 2.9 UVB 100mJ/㎠ 100.0 0.0 Vitamin C 10 84.5 2.3 Eucommia bark extract 10 76.7 1.0 50 64.4 1.9 100 59.9 4.2

Sample Conc. (Μg/ml) TRPV4 Expression rate
(% UVB, Actin correction) Stdev. Control 76.3 1.3 UVB 100mJ/㎠ 100.0 0.0 Vitamin C 10 79.0 0.8 Geniposide 0.1 91.6 2.0 One 82.1 6.2 2.5 60.6 5.7 5 56.7 1.9 Aucubin 0.1 71.2 4.0 One 56.3 1.8 5 51.6 2.8 10 50.7 5.6

[Experimental Example 5: Confirming the skin cooling effect of the cedar bark extract]

In this experimental example, the expression of PGE2 synthase protein involved in the increase in skin temperature was measured in order to find out how the cedar bark extract of Example 1 and its active components, geniposide and aucuvin, affect the skin cooling effect.

After inoculating normal human dermal fibroblast cells in 6 well multidish and incubating for 24 hours, the medium of the cultured cells was replaced with HBSS, and then Infrared ray was irradiated for 20 minutes. Thereafter, the serum-free medium was replaced, and the samples were treated for each concentration to incubate for 24 hours. After separating the protein, the effect of inhibiting the expression of PGE2 synthase protein of the cephalobark bark extract and its active components, geniposide and aucuvin, was measured through SDS-PAGE. This is shown in Tables 7 and 8 below.

Sample Conc. (Μg/ml) PGE2 synthase expression rate
(% IR, GAPDH correction) Control 88.53 Infrared ray 100.00 IR + Eucommia bark extract 25 94.83 50 89.21 100 88.20 IR + Aucubin 1.75 96.27 3.5 94.77 7 73.89 IR + Geniposide 0.5 74.71 One 76.85 2 78.24

As shown in Table 7 and FIG. 8, the bark extract of the cedar tree and the indicator components geniposide and aucuvin inhibited the expression of increased PGE2 synthase protein by heat treatment. Therefore, it is considered that the extract of the bark of the cedar tree and the indicator components geniposide and aucuvin have skin cooling efficacy by inhibiting the expression of PGE2 synthase protein increased by heat treatment.

[Example 2: Preparation of a cosmetic composition containing a cedar bark extract]

In this example, a gel was prepared as an example of the cosmetic composition of the present invention.

A gel containing 5.0% by weight of the cedar bark extract of Example 1 was prepared. On the other hand, in order to confirm the efficacy of the gel containing the cedar tree bark extract, a gel (comparative example) without the cedar tree bark extract was prepared. Table 8 below shows the contents of the cosmetic composition of Example 2 and Comparative Example.

Composition of Example 2 and Comparative Example ingredient Example 2 Content
(weight%) Comparative Example Content
(weight%) Glycerin 10.0 10.0 Butylene Glycol 1.5 1.5 Acrylates/C10-30 Alkyl Acrylate Crosspolymer 0.5 0.5 PEG-60 Hydrogenated Castor Oil 0.5 0.5 Sodium Hydroxide (20% Sol.) 0.3 0.3 Water 81.6 86.6 EUCOMMIA ULMOIDES BARK EXTRACT 5.0 - 1,2-Hexanediol 0.2 0.2 Ethylhexylglycerin 0.1 0.1 Phenoxyethanol 0.2 0.2 incense 0.1 0.1 Total 100 100

[Example 3: Preparation of a cosmetic composition containing a cedar bark extract]

In this example, a cream was prepared as an example of the cosmetic composition of the present invention.

A cream containing 5.0% by weight of the extract of the bark tree of Example 1 was prepared. Table 9 below shows the contents of the cosmetic composition of Example 3 and Comparative Example.

Composition of Example 3 and Comparative Example ingredient Example 3 Content
(weight%) Comparative Example Content
(weight%) Glycerin 8.0 8.0 Butylene Glycol 2.0 2.0 Carbomer 0.5 0.5 Hydroxyethylcellulose 0.1 0.1 Sodium Hydroxide (20% Sol.) 0.3 0.3 Water 71.3 76.3 EUCOMMIA ULMOIDES BARK EXTRACT 5.0 - Cetearyl Olivate, Sorbitan Olivate 2.0 2.0 Glyceryl Stearate, PEG-100 Stearate 2.0 2.O Cyclopentasiloxane, Cyclohexasiloxane 3.0 3.0 Caprylic/Capric Triglyceride 5.0 5.0 1,2-Hexanediol 0.3 0.3 Ethylhexylglycerin 0.1 0.1 Phenoxyethanol 0.3 0.3 incense 0.1 0.1 Total 100 100

[Example 4: Preparation of cosmetic composition containing cedar bark extract]

In this example, sunscreen was prepared as an example of the cosmetic composition of the present invention.

A sunscreen containing 5.0% by weight of the extract of bark of the cedar tree of Example 1 was prepared. Table 10 below shows the contents of the cosmetic composition of Example 4 and Comparative Example.

Composition of Example 4 and Comparative Example ingredient Example 4 Content
(weight%) Comparative Example Content
(weight%) Glycerin 2.0 2.0 Butylene Glycol 5.0 5.0 Acrylates/C10-30 Alkyl Acrylate Crosspolymer 0.5 0.5 Titanium Dioxide 3.2 3.2 Zinc Oxide 1.8 1.8 Sodium Hydroxide (20% Sol.) 0.3 0.3 Water 60.4 65.4 EUCOMMIA ULMOIDES BARK EXTRACT 5.0 - 1,2-Hexanediol 0.3 0.3 Ethylhexylglycerin 0.1 0.1 Phenoxyethanol 0.3 0.3 incense 0.1 0.1 Ethylhexyl Methoxycinnamate 7.5 7.5 Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine 3.0 3.0 Diethylamino Hydroxybenzoyl Hexyl Benzoate 1.0 1.0 C12-15 Alkyl Benzoate 5.0 5.0 Cetearyl Olivate, Sorbitan Olivate 3.5 3.5 Polyacrylate-13, Polyisobutene, Polysorbate 20, Sorbitan isostearate 1.0 1.0 Total 100 100

[Example 5: Preparation of cosmetic composition containing cedar bark extract]

In this example, BB cream was prepared as an example of the cosmetic composition of the present invention.

A BB cream containing 5.0% by weight of the extract of the bark tree of Example 1 was prepared. Table 11 below shows the contents of the cosmetic composition of Example 5 and Comparative Example.

Composition of Example 5 and Comparative Example ingredient Example 4 Content
(weight%) Comparative Example Content
(weight%) Glycerin 5.0 5.0 Butylene Glycol 5.0 5.0 NaCl 1.0 1.0 1,2-Hexandiol 0.9 0.9 Ethylhexylglycerin 0.1 0.1 Water 42.9 47.9 EUCOMMIA ULMOIDES BARK EXTRACT 5.0 - Polyglyceryl-2 Oleate, Polyhydroxystearic acid,
Polyglyceryl-2 Stearate 5.0 5.0 Beeswax 2.0 2.0 Sorbitan Olivate 1.0 1.0 Polyglyceryl-3 Polyricinoleate 2.0 2.0 Dicaprylyl Ether 10.0 10.0 Color Base 20.0 20.0 incense 0.1 0.1 Total 100 100

As in Examples 2 to 5, it is possible to prepare a cosmetic composition containing a cedar bark extract. Table 12 below shows classifications in the cosmetic compositions of Examples 2 to 5.

Foundation Solubilization type (serum) Example 2 Oil type (cream) Example 3 Dispersion type (sun cream) Example 4 hue BB cream Example 5

[Experimental Example 6: Confirmation of the effect of inhibiting skin surface temperature and the effect of inhibiting skin blood flow of the cosmetic composition of the present invention]

In this experimental example, as an example of the cosmetic compositions of Examples 2 to 5, it was intended to confirm the skin surface temperature inhibitory ability and skin blood flow inhibitory ability of the cosmetic composition containing the extract of the cedar bark of Example 2 as an example.

(1) Subject Basic Information

Table 9 shows information about the subjects who participated in this human application test.

Number of subjects participating in the test (persons) 21 people Number of subjects completed (persons) 20 (1 dropout: no follow-up) Overall mean age (standard deviation) 31.5 ± 14.63 years gender female

(2) Skin surface temperature measurement result

After using the product for 2 weeks, we visited the test institute and measured before (before) and 2 weeks (2w) after using the product. Experimental temperature to artificially increase the skin temperature using an infrared lamp after 2 weeks of using the product After setting the conditions (After IR), changes in skin surface temperature were measured. Skin surface temperature measurement results are shown in Tables 10 and 9 below.

Average skin surface temperature (average ± standard error) Measurement time Before (℃) 2w (℃) After IR (℃) Average Example 2 33.4±0.36 32.2±0.28 ^a,c 35.2±0.22 ^d Comparative example 34.1±0.48 33.9±0.54 ^b 36.2±0.20

^a : Example 2, Before vs. 2w: paired t-test, significant: p<0.001

^b : Comparative example, Before vs. 2w: paired t-test, no significant

^c : 2w, Example 2 vs. Comparative Example: independent t-test, significant: p<0.05

^d : After IR, Example 2 vs. Comparative Example: independent t-test, significant: p<0.05

As shown in Table 10 and Figure 9, Example 2 product use group was statistically significantly lower in skin surface temperature 2 weeks after use compared to before use, Comparative Example use group compared to before use and after 2 weeks of use statistics There was no difference. The skin surface temperature of the Example 2 use group was statistically significantly lower at the 2nd week of product use, and the skin surface temperature of the Example 2 use group was compared and evaluated after heat treatment of the two groups at the 2nd week of product use. The temperature was statistically significantly lower.

Therefore, it was confirmed that the extract of bark beetle can be used as a substance exhibiting the effect of inhibiting skin temperature.

(3) Skin blood flow measurement result

After using the product for 2 weeks, we visited the test institute and measured before (before) and 2 weeks (2w) after using the product. Experimental temperature to artificially increase the skin temperature using an infrared lamp after 2 weeks of using the product After setting the conditions (After IR), changes in skin blood flow were measured. Skin blood flow measurement results are shown in Table 11 and Figure 10 below.

Measurement time Before (℃) 2w (℃) After IR (℃) Average Example 2 216±16.2 181±18.3 ^a,c 230±25.6 ^d Comparative example 256±17.3 259±21.3 ^b 334±31.0

^a : Example 2, Before vs. 2w: paired t-test, significant: p<0.05

^b : Example 3, Before vs. 2w: paired t-test, no significant

^c : 2w, Example 2 vs. Comparative Example: independent t-test, significant: p<0.05

^d : After IR, Example 2 vs. Comparative Example: independent t-test, significant: p<0.05

As shown in Table 11 and Fig. 10, Example 2 product use group was statistically significantly lower in skin blood flow 2 weeks after use compared to before use, and Comparative Example use group was statistically used 2 weeks after use compared to before use There was no difference. Skin blood flow of the Example 2 use group was statistically significantly lower at the 2nd week of product use, and after comparing the blood flow after heat treatment of the 2 groups at the 2nd week of product use, the skin blood flow of the Example 2 use group was statistically low. Significantly lower. Therefore, it was confirmed that the cedar bark extract can be used to alleviate redness of the skin as a substance exhibiting the effect of inhibiting skin blood flow.

Summarizing the above, the cedar bark extract of the present invention and its active ingredients, geniposide and aucuvin, have anti-inflammatory effects by inhibiting the apoptosis-inhibiting effect, heat protection efficacy, and expression of PGE2 synthase protein increased by heat treatment. Confirmed. In addition, the cosmetic composition containing the cedar bark extract was confirmed to have excellent skin temperature inhibitory effect and skin blood flow inhibitory effect, which can be seen that due to the reduction of temperature control molecules such as PGE2 of the cedar bark extract and its active ingredient. .

Claims (11)

delete Contains Eucommia Ulmoides bark extract,
The cedar bark extract includes Geniposide and Aucubin, inhibits apoptosis by UV light, reduces the expression of TRPV4 increased by UV light, and increases PGE2 synthase by external heat A cosmetic composition for inhibiting skin temperature rise due to external stimulation, characterized by inhibiting protein expression.
delete According to claim 2,
The bark tree bark extract,
Cosmetic composition, characterized in that contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.
delete delete delete delete delete delete According to claim 2,
The cosmetic composition,
Skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion , Cleansing cream, body lotion or cosmetic composition characterized in that it is at least one formulation selected from the group consisting of a body cleanser.

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