WO2020085633A1 - Cosmetic composition comprising hardy rubber tree bark extract for cooling skin or reducing skin redness - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a substance derived from a natural product. More particularly, the present invention provides a cosmetic composition for reducing skin redness or for cooling skin and a preparation method therefor, the composition using an extract from hardy rubber tree bark and geniposide and aucubin, which are both contained in the extract from hardy rubber tree bark, to reduce skin redness by lowering the heat due to external exposure or to cool the skin by suppressing an increase in skin temperature.

Description

Cosmetic composition for skin cooling or skin flushing relief containing the extract of bark tree bark

The present invention relates to a cosmetic composition for skin cooling or skin flushing relief containing an extract of bark beetle.

The skin covers the surface of the human body with an area that occupies about 16% of the human body, and is in direct contact with the external environment, which is closely related to external factors such as temperature and humidity. The optimal body temperature of the body is 36.5 ℃, and the skin is 5 ~ 6 ℃ and 31 ℃, which is low. If the temperature is too high or too low, the skin health may deteriorate.

Recently, the Earth has renewed its highest temperature since meteorological observations, and it has been analyzed that high temperatures have persisted in Korea and around the world, and that even if current climate policies are implemented, it will lead to warming of over 3 ℃. As the temperature of the earth rises, the temperature of the skin also rises due to external environments such as ultraviolet rays and infrared rays, thereby accelerating skin aging. Therefore, it is necessary to keep the proper skin temperature from the skin temperature caused by external stimuli.

The increase in skin and body temperature is a complex reaction involving both the peripheral immune system and the brain, and in the normal thermal pathway, body temperature rises with the production of Prostaglandin E2 (PGE2). PGE2 is a major mediator of fever. It binds to the receptors of thermoregulatory neurons located in the anterior hypothalamus and exhibits a fever action. Skin redness and swelling by increasing blood flow to the infected tissue through vasodilation and increased microvascular permeability by PGE2 This will happen. In this regard, in the experiment using a 3D artificial skin model, the study of increasing the production of PGE2 by thermal stimulation and the expression of PGE synthase (PGES) and the expression of heat shock protein (HSP) -70 induced therein were also studied. There is a bar.

However, research using natural materials for controlling skin heat in relation to PGE2 is insignificant. Accordingly, the present invention is to provide a cosmetic composition for skin flushing relief or skin cooling by containing natural plant extracts to control skin heat due to external stimulation.

An object of the present invention is to find and extract an extract of a natural product excellent in skin flushing relief and skin cooling effect caused by heat by controlling skin heat caused by external stimulation, and to develop and provide a cosmetic composition containing it as an active ingredient. .

The present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains an extract of Eucommia ulmoides bark.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains an extract of Eucommia ulmoides bark.

On the other hand, in the present invention, the bark bark extract may be, preferably, to inhibit the expression of PGE2 synthetase protein.

On the other hand, in the present invention, the bark tree bark extract, preferably may be contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.

In addition, the present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains as an active ingredient Geniposide.

In addition, the present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains Aucubin as an active ingredient.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains a geniposide (Geniposide) as an active ingredient.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains aucubin (Aucubin) as an active ingredient.

On the other hand, in the present invention, the geniposide (Geniposide) or Aucubin (Aucubin), may preferably be to inhibit the expression of PGE2 synthase protein.

On the other hand, in the present invention, the geniposide (Geniposide) or Aucubin (Aucubin) may be preferably contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.

On the other hand, in the present invention, the cosmetic composition, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizer, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, It may be one or more formulations selected from the group consisting of nutrient essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion or body cleanser.

The present invention provides a cosmetic composition for skin cooling and a method for manufacturing the skin cooling to suppress redness caused by externally exposed heat and to increase the skin temperature by using the extract of the cedar bark extract and the geniposide and aucubin contained in the cedar bark extract do.

Anti-inflammatory effect by inhibiting the expression of PGE2 synthetase protein increased by the apoptosis bark extract of the present invention, the cell death inhibition effect, heat protection efficacy and heat treatment using geniposide and aucuvin contained in the cedar bark extract Can represent

1 is a graph showing the results of Geniposide analysis using high performance liquid chromatography (HLPC).

Figure 2 is a graph of the results of the analysis of the content of Geniposide (Geniposide) in the extract of bark beetle bark purity analyzed using high performance liquid chromatography (HLPC).

3 is a graph of the results of Aucubin analysis using high performance liquid chromatography (HLPC).

Figure 4 is a graph of the analysis results of the content of Aucubin (Aucubin) in the extract of the cedar tree bark purity analyzed using high performance liquid chromatography (HLPC).

5 is a graph showing the results of the HaCaT cytotoxicity test through MTT assay for the control group and the cedar bark extract and the active ingredients Geniposide and Aucubin.

6 is a photograph comparing the degree of staining according to JC-1 staining with respect to the control group and the extract of the cedar bark and the active ingredients Geniposide and Aucubin.

7 is a graph comparing the mRNA expression level of the related gene TRPV4 after irradiation with UVB for the control group and the cedar bark extract and the active ingredients Geniposide and Aucubin.

8 is a graph comparing the expression level of PGE2 synthetase protein for the control group and the cedar bark extract and the active ingredients Geniposide and Aucubin.

Figure 9 shows a comparison graph of the skin surface temperature according to before and after the use of each product for the gel group containing the extract of the bark tree bark (Example 2) and the gel group containing the bark tree bark extract (comparative example) before and after the use of each product. .

Figure 10 shows a comparison graph of skin blood flow according to before and after the use of each product for the group using the gel containing the cedar bark extract (Example 2) and the product using the gel containing the cedar bark extract (comparative example).

Eucommia ulmoides is a deciduous tree belonging to the family Nephi , and its leaves are oval-shaped, and the fruit is cut into a flat long circle to produce a rubbery, viscous thread, and the dried bark is used as a medicinal herb. The bark of the wormwood tree is called wormworm, and the young leaves are cotton, and is used for medicinal purposes.

The present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains an extract of Eucommia ulmoides bark.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains an extract of Eucommia ulmoides bark.

In the cosmetic composition of the present invention, the cedar bark extract can use various extraction methods commonly used in the art. For example, purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane solvent, respectively, or a mixture of two or more of them can be extracted using a mixed solvent as an extraction solvent.

In the present invention, after drying and crushing the bark of the cedar tree, an extract of the cedar tree bark is obtained using ethanol, filtered through a filter and concentrated, and then the cedar tree bark extract prepared by adding butylene glycol Used.

In the cosmetic composition of the present invention, the bark bark extract may be one that inhibits the expression of PGE2 synthase protein.

Prostaglandin E2 (Prostaglandin E2, PGE2) is a type of bioactive compound synthesized in vivo, and acts as a main medium for fever, binding to the receptors of thermoregulatory neurons in the anterior hypothalamus and exerting a role in increasing body temperature. Do it. In addition, through the expansion of blood vessels and increased microvascular permeability by PGE2, blood flow to the infected tissues increases, resulting in skin flushing and edema.

In the cosmetic composition of the present invention, the bark tree bark extract is preferably contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.

In addition, the present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains as an active ingredient Geniposide.

Geniposide is a substance present in about 40 kinds of natural plants as a component of iridoid glycosides, and is known to exhibit pharmacological effects such as anti-inflammatory, antioxidant, and immunomodulatory properties.

In addition, the present invention provides a cosmetic composition for alleviating flushing, characterized in that it contains Aucubin as an active ingredient.

Aucubin is a component found in natural plants as a component of iridoid glycosides, and is known to exhibit pharmacological effects in protecting liver damage, enhancing immunity, and treating gout.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains a geniposide (Geniposide) as an active ingredient.

In addition, the present invention provides a cosmetic composition for inhibiting skin temperature rise, characterized in that it contains aucubin (Aucubin) as an active ingredient.

In the cosmetic composition of the present invention, the geniposide or Aucubin may inhibit the expression of PGE2 synthase protein.

In the cosmetic composition of the present invention, the geniposide (Geniposide) or Aucubin (Aucubin) is preferably contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.

In the cosmetic composition of the present invention, the cosmetic composition, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence , Nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion or may be one or more formulations selected from the group consisting of a body cleanser.

In addition, the cosmetic composition of the present invention can be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may be varied according to a user's skin condition or taste.

Hereinafter, the present invention will be described in more detail in the following Examples and Experimental Examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes all modifications of equivalent technical ideas.

[Example 1: Preparation of cedar bark extract]

After drying and grinding the bark of the cedar tree, 50% ethanol in 50% ethanol: 50% ethanol = 1: 5, preferably 1: 8 by weight ratio of 30 to 80 ° C for 1 to 5 days, preferably 50 It was stirred for 1 day at ℃ to obtain an extract of bark beetle. Next, the cedar bark extract was filtered with a filter having a mesh size of 0.1 to 10 um, preferably 1 um to 0.3 um. The filtered bark tree bark extract was concentrated at a temperature of approximately 40-70 ° C, preferably 60 ° C to prepare a concentrate. Butylene glycol was added to the prepared concentrate to add 10 to 50% by weight, preferably 30% by weight, to prepare a cedar bark extract.

[Experimental Example 1: Analysis of the content of the bark extract of the cedar tree]

In this experimental example, the extracts of Example 1 were analyzed for the contents of Table 1 (Zeniposide) and Table 2 (Aucubin), which are effective components of the extract of bark beetle. Purity analysis was performed using high performance liquid chromatography (HPLC) under the described conditions, and the results are shown in Table 3 and FIGS. 1, 2, 3, and 4 below.

Purity analysis conditions using high performance liquid chromatography (HLPC) for the analysis of the content of Geniposide

Column	Capcell Pak C18 4.6 X 250mm (Shiseido HPLC Column)
Column temperature	35 ℃
Flow rate	1.0mL / min
Detector	UV 239nm
Analysis time	30 min
Injection Volume	20 μl
Mobile phase	Acetonitrile: Deionize Water = 15: 85

Purity analysis conditions using high performance liquid chromatography (HLPC) for the analysis of the content of Aucubin

Column	Capcell Pak C18 4.6 X 250mm (Shiseido HPLC Column)
Column temperature	35 ℃
Flow rate	0.5mL / min
Detector	UV 210nm
Analysis time	30 min
Injection Volume	20 μl
Mobile phase	Acetonitrile: Deionize Water = 5: 95

Purity analysis results using high performance liquid chromatography (HLPC)

Indicator	Duchung tree extract (ppm)
Geniposide	250 to 350
Aucubin	700 ~ 1,200

[Experimental Example 2: Cytotoxicity evaluation of bark tree bark extract (MTT assay)]

In this experimental example, the cytotoxicity of the extract of the cedar bark of Example 1 and its active components, geniposide and aucubin, were measured.

After inoculating keratinocytes (HaCaT) in a 24-well plate and incubating for 24 hours, the medium of the cultured cells was replaced with serum free medium, and the samples were treated by concentration to incubate for 24 hours. After the reaction was performed by replacing the cultured cells with a medium diluted with 10 times of MTT solution of 2.5 mg / ml, the supernatant was removed, and 1 ml of DMSO was added to dissolve the resulting MTT-formazan crystals, absorbance at 570 nm with ELISA Reader. Was measured. Cytotoxicity is expressed as a percentage of absorbance compared to the control, which is shown in Table 4 below.

As shown in Table 4 and Figure 5 below, as a result of performing a HaCaT cytotoxicity test through MTT assay to determine the sample concentration for the evaluation of the efficacy of the cedar bark extract and the indicator components geniposide and acubin, cedar bark extract Silver was toxic at a concentration of 250 ug / ml, and Geniposide and Aucubin were not toxic.

Sample	Conc. (Μg / ml)	Cell viability (%)	Stdev.
Control		100.0	0.8
Eucommia bark extract	One	101.0	2.8
	10	100.4	0.8
	25	97.8	2.7
	50	105.1	1.9
	100	102.8	1.2
	250	85.2	1.3
	500	61.9	1.2
Geniposide	0.1	101.6	1.3
	One	104.6	4.1
	2.5	101.9	0.8
	5	98.4	0.8
	10	100.8	0.9
	25	97.5	2.2
	50	101.2	1.7
	100	96.3	1.0
Aucubin	0.1	100.4	1.7
	One	103.4	4.4
	2.5	102.2	3.1
	5	101.7	4.7
	10	100.0	2.9
	25	105.9	2.4
	50	105.2	4.6
	100	96.2	2.1

[Experimental Example 3: Confirming the light-protecting efficacy of the cedar bark extract]

In this experimental example, the light-protecting efficacy of the cedar bark extract of Example 1 and its active components, geniposide and aucuvin, were measured.

To verify the mitochondrial cell membrane potential change, keratinocytes (Human keratinocytes) were dispensed into a 35 mm plate, stabilized for 24 hours, replaced with serum-free medium, and treated with samples. After 1 hour, after washing with HBSS (Hank's Balanced Salt solution) buffer, fresh HBSS was added and UVB 50 mJ / cm 2 was irradiated. Then, after replacing the medium with a serum-free medium, the sample was treated and incubated for 24 hours in a 37 ° C, 5% CO2 incubator. After removing the medium, 1 μg / ml of JC-1 solution was treated and reacted for 2 hours without light in a 37 ° C, 5% CO2 incubator to remove the medium and observed under a fluorescence microscope.

As a result of confirming the degree of staining of the extract from the bark tree bark and its active components, geniposide and aucubin, as compared to the control group UVB, as shown in FIG. 6, in Example 1, the effect of inhibiting apoptosis at 100 ug / ml was found. appear. The geniposide showed efficacy in the entire range of the treated concentration, and it was confirmed that the red fluorescence and the number of cells significantly increased compared to the UVB irradiation group, especially at the low concentration. Aucuvin showed efficacy at a concentration of 10 ug / ml or less. Therefore, it is believed that the extract of bark beetle and indicator components geniposide and aucuvin have high efficacy in inhibiting apoptosis by UVB.

[Experimental Example 4: Confirmation of the heat protection efficacy of the bark extract of cephalobacteria]

In this experimental example, the effect of heat protection of the skin was measured through the extract of the bark of the cedar tree of Example 1 and its active components, Jenny Poside and Aucubin.

After inoculating HEKn cells in 6 well multidish and incubating for 24 hours, the medium of the cultured cells was replaced with HBSS and UVB 100 mJ / cm 2 was investigated. After that, the cells were replaced with serum-free medium, and the samples were treated for each concentration to incubate for 24 hours. Cells were collected with TRIzol and RNA was isolated according to the manufacturer's method. After quantifying the isolated RNA, cDNA was synthesized with 2 μg of RNA to perform real-time PCR. This is shown in Tables 5 and 6 below.

As a result of the Real-Time PCR experiment, as shown in FIG. 7, the cedar bark extract reduced the expression of TRPV4 increased by UVB in a concentration-dependent manner, and decreased the expression of TRPV4 and geniposide and acubindo used as active ingredients. Therefore, it is considered that the extract of bark of the cedar tree, and the indicator components geniposide and aucuvin have a heat protection effect to protect the skin from being damaged by ultraviolet rays.

Sample	Conc. (Μg / ml)	TRPV4 Expression rate (% UVB, Actin correction)	Stdev.
Control		74.4	2.9
UVB 100mJ / ㎠		100.0	0.0
Vitamin C	10	84.5	2.3
Eucommia bark extract	10	76.7	1.0
	50	64.4	1.9
	100	59.9	4.2

Sample	Conc. (Μg / ml)	TRPV4 Expression rate (% UVB, Actin correction)	Stdev.
Control		76.3	1.3
UVB 100mJ / ㎠		100.0	0.0
Vitamin C	10	79.0	0.8
Geniposide	0.1	91.6	2.0
	One	82.1	6.2
	2.5	60.6	5.7
	5	56.7	1.9
Aucubin	0.1	71.2	4.0
	One	56.3	1.8
	5	51.6	2.8
	10	50.7	5.6

[Experimental Example 5: Confirming the cooling effect of the skin of the cedar bark extract]

In this experimental example, the expression of PGE2 synthase protein involved in the increase in skin temperature was measured in order to find out how the cedar bark extract of Example 1 and its active ingredients, Jenny Poside and Aucubin, affect the skin cooling effect.

After inoculating normal human dermal fibroblast cells in 6 well multidish and incubating for 24 hours, the medium of the cultured cells was replaced with HBSS, and then Infrared ray was irradiated for 20 minutes. Thereafter, the serum-free medium was replaced, and the samples were treated for each concentration to incubate for 24 hours. After separating the protein, the efficacy of inhibiting the expression of PGE2 synthase protein of the cephalobark bark extract and its active components, geniposide and Aucubin, was measured through SDS-PAGE. This is shown in Tables 7 and 8 below.

Sample	Conc. (Μg / ml)	PGE2 synthase expression rate (% IR, GAPDH correction)
Control		88.53
Infrared ray		100.00
IR + Eucommia bark extract	25	94.83
	50	89.21
	100	88.20
IR + Aucubin	1.75	96.27
	3.5	94.77
	7	73.89
IR + Geniposide	0.5	74.71
	One	76.85
	2	78.24

As shown in Tables 7 and 8, the extract of bark beetle and the indicator components geniposide and acubin inhibited the expression of increased PGE2 synthase protein by heat treatment. Therefore, it is believed that the extract of bark beetle and the indicator components geniposide and aucuvin have skin cooling efficacy by inhibiting the expression of PGE2 synthase protein increased by heat treatment.

[Example 2: Preparation of a cosmetic composition containing a cedar bark extract]

In this example, a gel was prepared as an example of the cosmetic composition of the present invention.

A gel containing 5.0% by weight of the cedar bark extract of Example 1 was prepared. On the other hand, in order to confirm the efficacy of the gel containing the cedar tree bark extract, a gel (comparative example) without the cedar tree bark extract was prepared. Table 8 below shows the contents of the cosmetic composition of Example 2 and Comparative Example.

Composition of Example 2 and Comparative Example

ingredient	Example 2 Content (% by weight)	Comparative example content (% by weight)
Glycerin	10.0	10.0
Butylene Glycol	1.5	1.5
Acrylates / C10-30 Alkyl Acrylate Crosspolymer	0.5	0.5
PEG-60 Hydrogenated Castor Oil	0.5	0.5
Sodium Hydroxide (20% Sol.)	0.3	0.3
Water	81.6	86.6
EUCOMMIA ULMOIDES BARK EXTRACT	5.0	-
1,2-Hexanediol	0.2	0.2
Ethylhexylglycerin	0.1	0.1
Phenoxyethanol	0.2	0.2
incense	0.1	0.1
Total	100	100

[Example 3: Preparation of a cosmetic composition containing a cedar bark extract]

In this example, a cream was prepared as an example of the cosmetic composition of the present invention.

A cream containing 5.0% by weight of the extract of the bark tree of Example 1 was prepared. Table 9 below shows the contents of the cosmetic composition of Example 3 and Comparative Example.

Composition of Example 3 and Comparative Example

ingredient	Example 3 Content (% by weight)	Comparative example content (% by weight)
Glycerin	8.0	8.0
Butylene Glycol	2.0	2.0
Carbomer	0.5	0.5
Hydroxyethylcellulose	0.1	0.1
Sodium Hydroxide (20% Sol.)	0.3	0.3
Water	71.3	76.3
EUCOMMIA ULMOIDES BARK EXTRACT	5.0	-
Cetearyl Olivate, Sorbitan Olivate	2.0	2.0
Glyceryl Stearate, PEG-100 Stearate	2.0	2.O
Cyclopentasiloxane, Cyclohexasiloxane	3.0	3.0
Caprylic / Capric Triglyceride	5.0	5.0
1,2-Hexanediol	0.3	0.3
Ethylhexylglycerin	0.1	0.1
Phenoxyethanol	0.3	0.3
incense	0.1	0.1
Total	100	100

[Example 4: Preparation of a cosmetic composition containing a cedar bark extract]

In this embodiment, sunscreen was prepared as an example of the cosmetic composition of the present invention.

A sunscreen was prepared containing 5.0% by weight of the extract of the bark tree of Example 1 above. Table 10 below shows the contents of the cosmetic composition of Example 4 and Comparative Example.

Composition of Example 4 and Comparative Example

ingredient	Example 4 Content (% by weight)	Comparative example content (% by weight)
Glycerin	2.0	2.0
Butylene Glycol	5.0	5.0
Acrylates / C10-30 Alkyl Acrylate Crosspolymer	0.5	0.5
Titanium Dioxide	3.2	3.2
Zinc Oxide	1.8	1.8
Sodium Hydroxide (20% Sol.)	0.3	0.3
Water	60.4	65.4
EUCOMMIA ULMOIDES BARK EXTRACT	5.0	-
1,2-Hexanediol	0.3	0.3
Ethylhexylglycerin	0.1	0.1
Phenoxyethanol	0.3	0.3
incense	0.1	0.1
Ethylhexyl Methoxycinnamate	7.5	7.5
Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine	3.0	3.0
Diethylamino Hydroxybenzoyl Hexyl Benzoate	1.0	1.0
C12-15 Alkyl Benzoate	5.0	5.0
Cetearyl Olivate, Sorbitan Olivate	3.5	3.5
Polyacrylate-13, Polyisobutene, Polysorbate 20, Sorbitan isostearate	1.0	1.0
Total	100	100

[Example 5: Preparation of cosmetic composition containing cedar bark extract]

In this example, BB cream was prepared as an example of the cosmetic composition of the present invention.

A BB cream containing 5.0% by weight of the extract of the bark tree of Example 1 was prepared. Table 11 below shows the contents of the cosmetic composition of Example 5 and Comparative Example.

Composition of Example 5 and Comparative Example

ingredient	Example 4 Content (% by weight)	Comparative example content (% by weight)
Glycerin	5.0	5.0
Butylene Glycol	5.0	5.0
NaCl	1.0	1.0
1,2-Hexandiol	0.9	0.9
Ethylhexylglycerin	0.1	0.1
Water	42.9	47.9
EUCOMMIA ULMOIDES BARK EXTRACT	5.0	-
Polyglyceryl-2 Oleate, Polyhydroxystearic acid, Polyglyceryl-2 Stearate	5.0	5.0
Beeswax	2.0	2.0
Sorbitan Olivate	1.0	1.0
Polyglyceryl-3 Polyricinoleate	2.0	2.0
Dicaprylyl Ether	10.0	10.0
Color Base	20.0	20.0
incense	0.1	0.1
Total	100	100

As in Examples 2 to 5, it is possible to prepare a cosmetic composition containing a cedar bark extract. Table 12 below shows classifications in the cosmetic compositions of Examples 2 to 5.

Foundation	Solubilization type (serum)	Example 2
	Oil type (cream)	Example 3
	Dispersion type (sun cream)	Example 4
hue	BB cream	Example 5

[Experimental Example 6: Confirmation of the effect of inhibiting skin surface temperature and the effect of inhibiting skin blood flow of the cosmetic composition of the present invention]

In this experimental example, as an example of the cosmetic compositions of Examples 2 to 5, it was intended to confirm the skin surface temperature inhibitory ability and skin blood flow inhibitory ability of the cosmetic composition containing the extract of the cedar bark of Example 2 as an example.

(1) Subject Basic Information

Table 9 shows information about the subjects who participated in this human application test.

Number of subjects participating in the test (persons)	21 people
Number of subjects completed (persons)	20 (1 dropout: no follow-up)
Overall mean age (standard deviation)	31.5 ± 14.63 years
gender	female

(2) Skin surface temperature measurement result

After using the product for 2 weeks, we visited the test institute and measured before (before) and 2 weeks (2w) after using the product, and after 2 weeks of using the product, the experimental temperature to artificially increase the skin temperature using an infrared lamp After setting the conditions (After IR), changes in skin surface temperature were measured. Skin surface temperature measurement results are shown in Tables 10 and 9 below.

Average skin surface temperature (average ± standard error)

Measurement time		Before (℃)	2w (℃)	After IR (℃)
Average	Example 2	33.4 ± 0.36	32.2 ± 0.28 a, c	35.2 ± 0.22 d
	Comparative example	34.1 ± 0.48	33.9 ± 0.54 b	36.2 ± 0.20

^a : Example 2, Before vs. 2w: paired t-test, significant: p <0.001

^b : Comparative Example, Before vs. 2w: paired t-test, no significant

^c : 2w, Example 2 vs. Comparative Example: independent t-test, significant: p <0.05

^d : After IR, Example 2 vs. Comparative Example: independent t-test, significant: p <0.05

As shown in Table 10 and Figure 9, Example 2 product use group was statistically significantly lower in skin surface temperature after 2 weeks of use compared to before use, and Comparative Example use group was compared after use for 2 weeks after use. There was no difference. The skin surface temperature of the Example 2 use group was statistically significantly lower at the 2nd week of product use, and the skin surface temperature of the Example 2 use group was compared and evaluated after heat treatment of the two groups at the 2nd week of product use. The temperature was statistically significantly lower.

Thus, it was confirmed that the extract of bark beetle can be used as a substance exhibiting the effect of inhibiting skin temperature.

(3) Skin blood flow measurement result

After using the product for 2 weeks, we visited the test institute and measured before (before) and 2 weeks (2w) after using the product, and after 2 weeks of using the product, the experimental temperature to artificially increase the skin temperature using an infrared lamp After setting the conditions (After IR), changes in skin blood flow were measured. Skin blood flow measurement results are shown in Table 11 and Figure 10 below.

Measurement time		Before (℃)	2w (℃)	After IR (℃)
Average	Example 2	216 ± 16.2	181 ± 18.3 a, c	230 ± 25.6 d
	Comparative example	256 ± 17.3	259 ± 21.3 b	334 ± 31.0

^a : Example 2, Before vs. 2w: paired t-test, significant: p <0.05

^b : Example 3, Before vs. 2w: paired t-test, no significant

^c : 2w, Example 2 vs. Comparative Example: independent t-test, significant: p <0.05

^d : After IR, Example 2 vs. Comparative Example: independent t-test, significant: p <0.05

As shown in Table 11 and Fig. 10, Example 2 product use group was statistically significantly lower in skin blood flow 2 weeks after use compared to before use, and Comparative Example use group was statistically used 2 weeks after use compared to before use There was no difference. In the second week of product use, the skin blood flow of the Example 2 use group was statistically significantly lower, and after the two groups were heat treated at the second week of product use, the blood flow was compared and evaluated, and the skin blood flow of the Example 2 use group was statistically low. Significantly lower. Therefore, it was confirmed that the cedar bark extract can be used to alleviate skin flushing as a substance exhibiting the effect of inhibiting skin blood flow.

Summarizing the above, the cedar bark extract of the present invention and its active ingredients, geniposide and aucuvin, have anti-inflammatory effects by inhibiting the apoptosis inhibitory effect, heat protection effect and expression of PGE2 synthase protein increased by heat treatment. Confirmed. In addition, it was confirmed that the cosmetic composition containing the cedar bark extract has excellent skin temperature inhibitory effect and skin blood flow inhibitory effect. .

Claims (11)

1. A cosmetic composition for alleviating flushing, characterized in that it contains an extract of Eucommia Ulmoides bark.
2. Cosmetic composition for inhibiting skin temperature rise, characterized in that it contains an extract of Eucommia Ulmoides bark.
3. According to any one of claims 1 and 2,

   The cedar bark extract,

   A cosmetic composition characterized by inhibiting the expression of PGE2 synthase protein.
4. According to any one of claims 1 and 2,

   The cedar bark extract,

   Cosmetic composition, characterized in that contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.
5. A cosmetic composition for relieving redness, characterized in that it contains Geniposide as an active ingredient.
6. A cosmetic composition for alleviating flushing, characterized in that it contains Aucubin as an active ingredient.
7. A cosmetic composition for inhibiting skin temperature rise, characterized in that it contains Geniposide as an active ingredient.
8. A cosmetic composition for inhibiting skin temperature rise, characterized in that it contains Aucubin (Aucubin) as an active ingredient.
9. The method according to any one of claims 5 to 8,

   The geniposide (Geniposide) or Aucubin (Aucubin),

   A cosmetic composition characterized by inhibiting the expression of PGE2 synthase protein.
10. The method according to any one of claims 5 to 8,

    The geniposide (Geniposide) or Aucubin (Aucubin),

    Cosmetic composition, characterized in that contained in 0.1 to 20% by weight relative to the total weight of the cosmetic composition.
11. The method of claim 1, 2, 5 to 8,

    The cosmetic composition,

    Skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion , Cleansing cream, body lotion or cosmetic composition, characterized in that at least one formulation selected from the group consisting of a body cleanser.

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