KR102135745B1 - 발현 카세트 - Google Patents

발현 카세트 Download PDF

Info

Publication number
KR102135745B1
KR102135745B1 KR1020147018672A KR20147018672A KR102135745B1 KR 102135745 B1 KR102135745 B1 KR 102135745B1 KR 1020147018672 A KR1020147018672 A KR 1020147018672A KR 20147018672 A KR20147018672 A KR 20147018672A KR 102135745 B1 KR102135745 B1 KR 102135745B1
Authority
KR
South Korea
Prior art keywords
gapdh
promoter
genomic dna
expression
dna sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020147018672A
Other languages
English (en)
Korean (ko)
Other versions
KR20140113666A (ko
Inventor
데니얼 로쉐
크리스텔 에비셔-거미
삐에르 모레띠
마틴 버츠친거
Original Assignee
아이크노스 사이언스 에스. 아.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 아이크노스 사이언스 에스. 아. filed Critical 아이크노스 사이언스 에스. 아.
Publication of KR20140113666A publication Critical patent/KR20140113666A/ko
Application granted granted Critical
Publication of KR102135745B1 publication Critical patent/KR102135745B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020147018672A 2011-12-07 2012-12-05 발현 카세트 Active KR102135745B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161567675P 2011-12-07 2011-12-07
US61/567,675 2011-12-07
PCT/IB2012/056977 WO2013084157A1 (en) 2011-12-07 2012-12-05 Expression cassette

Publications (2)

Publication Number Publication Date
KR20140113666A KR20140113666A (ko) 2014-09-24
KR102135745B1 true KR102135745B1 (ko) 2020-07-21

Family

ID=47561694

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020147018672A Active KR102135745B1 (ko) 2011-12-07 2012-12-05 발현 카세트

Country Status (15)

Country Link
US (3) US20130344604A1 (https=)
EP (1) EP2788483B1 (https=)
JP (3) JP2015501642A (https=)
KR (1) KR102135745B1 (https=)
CN (1) CN104093834A (https=)
AU (2) AU2012348558A1 (https=)
BR (1) BR112014013597A2 (https=)
CA (1) CA2856479A1 (https=)
EA (1) EA037273B1 (https=)
ES (1) ES2679291T3 (https=)
HK (1) HK1202578A1 (https=)
IL (1) IL232845A0 (https=)
MX (1) MX362393B (https=)
SG (1) SG11201402477VA (https=)
WO (1) WO2013084157A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX377591B (es) * 2014-10-15 2025-03-10 Amgen Inc Elementos promotores y reguladores para mejorar la expresión de genes heterólogos en células hospederas.
WO2016074016A1 (en) * 2014-11-10 2016-05-19 Murdoch Childrens Research Institute Vectors and methods for targeted integration in loci comprising constitutively expressed genes
CN106868044A (zh) * 2017-02-15 2017-06-20 扬州大学 一种转基因动物毛色报告基因表达盒、表达载体及应用
WO2019183248A1 (en) 2018-03-21 2019-09-26 Nature Technology Corporation Viral and non-viral nanoplasmid vectors with improved production
CN114540352A (zh) * 2022-02-16 2022-05-27 艾瑞生命科学技术(常州)有限公司 一种多核苷酸、载体元件及表达载体和宿主细胞及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0173552B1 (en) * 1984-08-24 1991-10-09 The Upjohn Company Recombinant dna compounds and the expression of polypeptides such as tpa
KR20000006334A (ko) * 1998-06-26 2000-01-25 이선경 바이러스코딩염기서열이전혀없는고효율레트로바이러스벡터
KR20030027947A (ko) * 2000-08-10 2003-04-07 오노 야꾸힝 고교 가부시키가이샤 세포사를 유도하는 글리세릴알데히드-3-인산 탈수소효소프로모터
WO2007068265A1 (en) * 2005-12-12 2007-06-21 Research Corporation Technologies, Inc. Hybrid 3’ untranslated regions suitable for efficient protein expression in mammalian cells

Also Published As

Publication number Publication date
AU2018204332A1 (en) 2018-07-05
EA037273B1 (ru) 2021-03-02
AU2018204332B2 (en) 2020-09-10
MX362393B (es) 2019-01-15
US20180163225A1 (en) 2018-06-14
KR20140113666A (ko) 2014-09-24
CN104093834A (zh) 2014-10-08
JP2015501642A (ja) 2015-01-19
JP2017200476A (ja) 2017-11-09
CA2856479A1 (en) 2013-06-13
ES2679291T3 (es) 2018-08-23
EP2788483B1 (en) 2018-06-27
IL232845A0 (en) 2014-07-31
EP2788483A1 (en) 2014-10-15
WO2013084157A1 (en) 2013-06-13
JP7096790B2 (ja) 2022-07-06
US20130344604A1 (en) 2013-12-26
SG11201402477VA (en) 2014-09-26
AU2012348558A1 (en) 2014-07-10
NZ626252A (en) 2016-06-24
MX2014006869A (es) 2014-07-14
US20200087679A1 (en) 2020-03-19
JP2019193659A (ja) 2019-11-07
EA201491034A1 (ru) 2015-04-30
HK1202578A1 (en) 2015-10-02
BR112014013597A2 (pt) 2020-08-18
AU2012348558A2 (en) 2014-07-24

Similar Documents

Publication Publication Date Title
KR102531749B1 (ko) Cho 세포 내 통합 부위
KR102907773B1 (ko) 인간 대상체에서의 비-노화-관련 청각 손상의 치료를 위한 조성물 및 방법
AU2017315679B2 (en) Compositions and methods for treating non-age-associated hearing impairment in a human subject
KR102301464B1 (ko) 종양 세포에 의한 면역 억제를 감소시키기 위한 방법 및 조성물
KR102235603B1 (ko) 향상된 이식유전자 발현 및 가공
WO2020097372A1 (en) Use of adeno-associated viral vectors to correct gene defects/ express proteins in hair cells and supporting cells in the inner ear
CN107941681B (zh) 鉴定生物样品中定量细胞组成的方法
KR101476010B1 (ko) 고생산성 세포의 수립을 위한 발현 벡터 및 고생산성 세포
RU2749715C2 (ru) Грызуны, характеризующиеся гуманизированным геном tmprss
HK1245010A1 (zh) 用於治疗疾病的遗传修饰的细胞、组织和器官
AU2018204332A1 (en) Expression cassette
AU2022200678B2 (en) Nucleic acid molecules containing spacers and methods of use thereof
KR20120099363A (ko) 탯줄 혈액으로부터의 유도 만능 줄기 세포의 생성
JP2025011135A (ja) 核酸の標的化組込み
TW202309274A (zh) 產生成熟角膜內皮細胞之方法
AU759689B2 (en) Novel hemopoietin receptor proteins
KR20230101839A (ko) 세이프 하버 유전자좌위
KR20240099242A (ko) 안정적인 통합 부위를 생산하기 위한 통합된 cas9 유전자를 포함하는 포유동물 세포, 및 안정적인 통합 부위 및 기타 부위를 포함하는 포유동물 세포
CN115175559A (zh) 包含人源化pnpla3基因座的非人动物及其使用方法
KR102827498B1 (ko) 포유류 세포들에서 젖분비자극 활성의 조절
AU2019318910A1 (en) Promotor for Hspa8 gene
CN113264996A (zh) 一种人源化的非人动物及其制备方法和应用
AU2023258798A1 (en) PROMOTER OF Eno1 GENE

Legal Events

Date Code Title Description
AMND Amendment
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

AMND Amendment
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PE0801 Dismissal of amendment

St.27 status event code: A-2-2-P10-P12-nap-PE0801

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

AMND Amendment
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E601 Decision to refuse application
PE0601 Decision on rejection of patent

St.27 status event code: N-2-6-B10-B15-exm-PE0601

X091 Application refused [patent]
AMND Amendment
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PX0901 Re-examination

St.27 status event code: A-2-3-E10-E12-rex-PX0901

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

E90F Notification of reason for final refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

AMND Amendment
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PX0701 Decision of registration after re-examination

St.27 status event code: A-3-4-F10-F13-rex-PX0701

X701 Decision to grant (after re-examination)
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

R15-X000 Change to inventor requested

St.27 status event code: A-3-3-R10-R15-oth-X000

R16-X000 Change to inventor recorded

St.27 status event code: A-3-3-R10-R16-oth-X000

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PN2301 Change of applicant

St.27 status event code: A-5-5-R10-R13-asn-PN2301

St.27 status event code: A-5-5-R10-R11-asn-PN2301

R11 Change to the name of applicant or owner or transfer of ownership requested

Free format text: ST27 STATUS EVENT CODE: A-5-5-R10-R11-ASN-PN2301 (AS PROVIDED BY THE NATIONAL OFFICE)

R13 Change to the name of applicant or owner recorded

Free format text: ST27 STATUS EVENT CODE: A-5-5-R10-R13-ASN-PN2301 (AS PROVIDED BY THE NATIONAL OFFICE)