KR102135739B1 - Forming mosquito repellant composition and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a mosquito repellent composition, and more particularly, to a foamed mosquito repellent composition and a method for manufacturing the mosquito attracting component and natural mosquito insecticidal component composed of the components of sweat to prepare a mosquito repellent according to the present invention Wow; Experimenting and evaluating the insecticidal and mosquito attraction effect of the mosquito repellent obtained in the above step; Since the present invention has been achieved through the step of manufacturing the mosquito repellent of the present invention as a foamed tablet, the present invention has an effect of providing a new mosquito repellent that is easily dissolved in water and is easy to move, and the inconvenience of liquid product It has an excellent effect of providing a stable and environmentally friendly mosquito repellent that is excluded.

Description

Foaming mosquito repellant composition and manufacturing method thereof

The present invention relates to a mosquito repellent composition, and more particularly, to a foamed mosquito repellent composition and a method for manufacturing the same.

Climate change due to global warming has increased the number of mosquitoes and the damage caused by them. Worldwide, 2 million people die each year from dengue fever, yellow fever, malaria, and Japanese encephalitis, and the number of patients in Korea is also increasing rapidly. The effective insecticidal component of domestic pesticides is a contact repellent, and pyrethroid-based insecticides such as permethrin, which are toxic to the nervous system of mosquitoes, occupy most of them. Representative chemical synthesis agents used as repellents and pesticides include permethrin, allerthrin, deltamethrin, and cypermethrin. Although the use of pesticides has increased, synthetic pesticides used in the past have problems such as drug resistance, killing of useful organisms, toxicity to humans and livestock, persistence, and various environmental pollution. Therefore, there is an urgent need to develop new alternative medicines and control methods that can effectively protect humans from mosquitoes while minimizing the impact on humans and ecosystems.

Mosquito detects attractants, such as lactic acid and carbon dioxide from humans, from the receptors of chemicals in the tactile senses, and finds targets for vampires. Mosquito repellents interfere with the detection of this receptor, preventing the transfer of kairomone (a substance produced by one organism in favor of another species) to the nerves in the mosquito's touch. That is the principle. Diethyltoluamide (N,N-Diethyl-meta-toluamide), also known as DEET, is one of the most commonly used pesticide ingredients in children's products. It affects the central nervous system and brain nerves, causing side effects such as seizures and paralysis during prolonged exposure. As a result, the EU has gradually ceased to use it since 2006, and WHO continues to send out restrictions on the use of pesticides through WHOPES to member countries.

For a pesticide for mosquito repellents of a foamed tablet type used by direct spraying or dilution in water of 100 to 10,000 times, it has been disclosed in Korean Patent Publication No. 10-2013-0107162, and a fragrance is added to the main component permethrin of the pesticide The pest repellent for aerosol use after dissolving in anhydrous ethanol has been disclosed in Korean Patent Registration No. 10-0485412, and is a mosquito repellent that can be used by finely encapsulating a single DEET material or by mixing a silver compound with a DEET material. Regarding, it has been disclosed in Patent No. 10-0955620. Natural pesticide components obtained by extracting from ginkgo biloba or ginkgo biloba with an alcoholic solvent and then concentration and fractional extraction have been disclosed in Publication No. 10-2013-0026742. However, none of the above-mentioned patent documents discloses or suggests mosquito insecticides that simultaneously perform mosquito attraction and insecticidal functions.

Accordingly, an object of the present invention is to provide a method for manufacturing a foam-type mosquito repellent.

Another object of the present invention is to provide a foam-type mosquito repellent prepared by the above method.

The object of the present invention comprises the steps of preparing a mosquito repellent according to the present invention by mixing mosquito attracting components and natural mosquito pesticide components composed of sweat components; Experimenting and evaluating the insecticidal and mosquito attraction effect of the mosquito repellent obtained in the above step; The present invention was achieved through the step of preparing a mosquito repellent into a foamed tablet.

Chrysanthemum indicum (Chrysanthemum indicum) is a perennial plant of the dicotyledonous family Chrysanthemum Chrysanthemum, also known as Hwangguk. It grows mainly in the mountains and has short hairs throughout the grass, and the stem is 60-90cm high and is thin and long in black. In October, the flowers are dried and drunk. Young leaves are used as herbs. The pyrethic component extracted from the chrysanthemum called chungchungguk is used as a natural insecticide. It also contains a small amount of pyrethic components in mountain, yellow, gingko, gukgeuk, gujeolcho, wormwood, and ants.

Turmeric is a perennial plant of the family Gingeraceae, which is mainly produced in southern regions such as Jindo, and the yellow crystalline diketone compound curcumin and its derivatives p-hydroxycinnamoylferuroylmethane and p-hydroxycinnamoylferuloylmethane and It contains 0.3% by weight of a yellow pigment of p,p'-dihydroxydicinnamoylmethane, which has been found to be effective against insect repellents such as mosquitoes.

Zephyr (Zanthoxylum piperitum) is a deciduous shrub with the dicotyledonous plant, also known as the bark tree, and grows at a height of 3 to 5 m in the middle and mountain valleys. The spicy ingredients and scent of the epidermis are almost non-toxic to humans, but very toxic to insects such as mosquitoes and flies, or animals such as fish, pigs and ducks.

Seshin is a medicine that has dried the roots of spiderweed, and its roots are extremely thin and have a very spicy taste. Spiderweed is a perennial herbaceous plant belonging to the coniferous family and has many nodes in the root, and two leaves appear at the end of the main stem and spread. The cold is severe, especially when the headache is severe and can't stand. In the private sector, it is said that if a tooth is bitten in a sore spot, it will soon become a pain reliever.

Sinihwa (辛夷花) is a mountain magnolia flower, and the name Shin (莘) has a slightly spicy taste. Shinhwa's taste is spicy and its properties are warm, and flowers, berries, leaves, roots, and shells are all used as medicine. The efficacy of Shin Ewha plays a role in piercing blocked things by passing through the wind and clears the head to cure freckles and freckles on the face.

The present invention has an effect of providing a new mosquito repellent that is easily dissolved and used in water, and has an excellent effect of providing a stable and eco-friendly mosquito repellent that is easy to move and excludes the inconvenience of a liquid state product.

1 is a view showing the manufacturing process of the foamed mosquito repellent according to the present invention.

The present invention comprises the steps of sieving after finely pulverizing each of chrysanthemum, turmeric, cortex, sesin, and shinhwa; After drying the mixed powder of the sifted chrysanthemum, turmeric, cortex tree, sessin and shinhwa micropowder in the above step for 2 hours at 50°C, glucose, 2-phenylethanol, lactic acid, octanal, myristic acid, 2-nonenal , Mixing with a mixture of trimethylamine and carbon dioxide; And adding an excipient and a lubricant to the mixed powder of the above step, and then inserting it into a tableting machine and molding the foamed mosquito repellent.

Excipients for the formation and maintenance of granules and tablets, which are added for the purpose of giving a suitable stiffness or shape to the drug, or for the purpose of providing a size that is easy to handle by giving a certain dose and weight when the amount of the subject is small. Lactose, hydroxypropyl cellulose and talc are mainly used. Sodium hydrogen carbonate and citric acid are used as excipients to form bubbles as they dissolve in water.

The present invention provides a foam-type mosquito repellent prepared by the above manufacturing method.

Hereinafter, specific details of the present invention will be described in detail with reference to examples and experimental examples.

Example: Preparation of the present invention mosquito repellent

Glucose, 2-phenylethanol, lactic acid, octanal, myristic acid, 2-nonenal, trimethylamine and carbon dioxide used in the present invention were purchased from the Sigma-Aldrich Internet homepage. The chrysanthemum, turmeric, cortex, sessin and shinhwa used in the present invention were purchased from specialized stores and finely ground using a Circoplex impact mill (Model 50-ZPS, Alpine Aktiengesellschaft). The pulverized product was used as a sample while being stored frozen at -80°C.

Composition of the present invention mosquito repellent Mosquito repellent of the present invention Mosquito attracting ingredient (A) Included (% by weight) Mosquito pesticide component (B) Included (% by weight) glucose 1.0 Chrysanthemum 18 2-phenylethanol 1.0 Turmeric 18 Lactic acid 1.2 Tree 18 Octalal 1.25 Seshin 18 Myristic acid 1.25 Shin Ewha 18 2-Normal 1.4 Trimethylamine 1.4 carbon dioxide 1.5 Sum 10.0 Sum 90.0 Total 100.0

According to the composition of Table 1, the mosquito attracting component (A) and the mosquito insecticidal component (B) were mixed at a weight ratio of 10:90 to prepare a mixed powder of the present invention mosquito repellent.

Experimental Example 1: Mosquito repellent of the present invention Insecticidal Experiment

With the mosquito repellent prepared in the above example, the pesticidal activity was tested in accordance with the following regulations determined by the World Health Organization (WHO), and the following half-numerical dose (LD ₅₀ ) was measured and shown in Table 2 below.

100 g of acetone was added to 10 g of the mosquito repellent mixture powder, and the mixture was sufficiently dissolved and used in each experiment. Red House Mosquito ( Culex pipiens pallens ) 60 adults were divided into three groups, and the first group was treated locally (Tropical application: mosquitoes anesthetized with carbon dioxide gas were sprayed with 0.5 μL of sample solution on the chest using a micro applicator) and the second group was watered. (Water feeding: Prepare a sample of acetone: 5% sucrose aqueous solution at a ratio of 1:9, soak it in a cotton patch, blow acetone in a space of 20cm×20cm×20cm, and incubate it with mosquitoes) to incubate the adult. After spinning, it was contacted for 30 minutes and then transferred to an observation net to investigate the mortality after 24 hours. Each experiment was repeated three times, and the control experiment without treatment with drugs was compared in parallel. The results were as shown in [Table 2].

Insecticidal power of the present invention mosquito repellent Number of experiments
Mosquito repellent insecticidal component (LD ₅₀ ) (mg/kg body weight) of the present invention Local application Watering group Control One 300 320 ― 2 305 310 ― 3 312 315 ―

Experimental Example 2: Experiment for confirming the attraction effect of the present invention mosquito repellent

On one side of a 50 cm x 50 cm x 50 cm transparent acrylic box, 0.5 g of a mosquito repellent mixture powder prepared in Example was dissolved in 500 mL of purified water. On the other side of the box, add 500 mL of purified water and 50 red house mosquitoes ( Culex pipiens pallens ) The mosquito incentive (%) was investigated by observing the mosquitoes' activity tendency by emitting adult insects for 24 hours.

The attractive force of the present invention mosquito repellent Number of experiments
Total mosquitoes
Mosquito repellent of the present invention Control Mosquitoes Attraction rate (%) Mosquitoes Attraction rate (%) One 50 47 94 3 6 2 50 48 96 2 4 3 50 48 96 2 4

As a result, more than 95% of mosquitoes were attracted to areas where mosquito repellent was dissolved.

Manufacturing example: foam Tablet Manufacturing method

After mixing the excipients consisting of a mixture of sodium carbonate, citric acid, lactose, hydroxypropylcellulose and talc in a weight ratio of the mosquito repellent mixture powder prepared in Example, after sieving by passing through a granule having a size of 20 mesh The granules were dried by drying at 60°C for 2 hours. After adding 1.5 g of a lubricant (100% magnesium stearate) to 50 g of the granules, 0.5 g of each was put into a tablet press making machine (KTP-12, KoreaMedi) and molded under a pressure of 200 kg/cm 2 to prepare a tablet.

The present invention is a very useful invention in the pest control industry because it has an excellent effect of providing an environmentally friendly mosquito repellent.

Claims (5)

(a) preparing a finely pulverized product by finely crushing each of chrysanthemum, turmeric, cortex, sesin, and shinhwa;
(b) preparing a mixture by mixing glucose, 2-phenylethanol, lactic acid, octanal, myristic acid, 2-nonenal, trimethylamine and carbon dioxide;
(c) preparing a blend by blending the fine pulverized material with the mixture;
(d) adding an excipient and a lubricant to the formulation, and then putting it in a tabletting machine and molding it to form a tablet;
In the step (c), a method of manufacturing a foamed mosquito repellent, characterized in that the mixture of the fine pulverized material and the mixture in a ratio of 9: 1 (w/w) to prepare a blend.
The method of claim 1, wherein the excipient is at least one selected from the group consisting of sodium carbonate, citric acid, lactose, hydroxypropylcellulose, and talc.
According to claim 1,
Step (a) is,
A method of producing a foam-type mosquito repellent, which is a step of finely pulverizing each of chrysanthemum, turmeric, cortex, sesins and shinhwa and then sieving and drying for 2 hours at 50°C.
delete A foam-type mosquito repellent prepared by the method of any one of claims 1 to 3.

Images