KR102079293B1 - 발현 서열 - Google Patents

발현 서열 Download PDF

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Publication number
KR102079293B1
KR102079293B1 KR1020157009776A KR20157009776A KR102079293B1 KR 102079293 B1 KR102079293 B1 KR 102079293B1 KR 1020157009776 A KR1020157009776 A KR 1020157009776A KR 20157009776 A KR20157009776 A KR 20157009776A KR 102079293 B1 KR102079293 B1 KR 102079293B1
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seq
amino acid
poi
leader
sequence
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KR20150076160A (ko
Inventor
브리짓 개서
디트하드 마타노비치
실비아 하이스
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론자 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020157009776A 2012-10-29 2013-10-29 발현 서열 Active KR102079293B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12190361.1 2012-10-29
EP12190361 2012-10-29
PCT/EP2013/072572 WO2014067926A1 (en) 2012-10-29 2013-10-29 Expression sequences

Publications (2)

Publication Number Publication Date
KR20150076160A KR20150076160A (ko) 2015-07-06
KR102079293B1 true KR102079293B1 (ko) 2020-02-19

Family

ID=47115533

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020157009776A Active KR102079293B1 (ko) 2012-10-29 2013-10-29 발현 서열

Country Status (16)

Country Link
US (2) US10160988B2 (https=)
EP (2) EP3130598B1 (https=)
JP (2) JP6556625B2 (https=)
KR (1) KR102079293B1 (https=)
CN (1) CN104837857B (https=)
AU (1) AU2013341049B2 (https=)
BR (1) BR112015008289A2 (https=)
CA (2) CA3212565A1 (https=)
DK (1) DK2912053T3 (https=)
ES (2) ES2610990T3 (https=)
HU (1) HUE033282T2 (https=)
IL (2) IL238420B (https=)
IN (1) IN2015DN02771A (https=)
PL (1) PL2912053T3 (https=)
SG (1) SG11201502745XA (https=)
WO (1) WO2014067926A1 (https=)

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* Cited by examiner, † Cited by third party
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AU2013341049B2 (en) * 2012-10-29 2017-07-20 Lonza Ltd Expression sequences
CN104312933B (zh) * 2014-10-17 2017-03-29 江南大学 一种优化信号肽提高胰蛋白酶胞外分泌表达的方法
DK3332005T3 (da) 2015-08-05 2022-01-03 Lonza Ag Promotorvarianter
US11377677B2 (en) 2016-03-02 2022-07-05 Lonza Ltd Fermentation process
KR20250121449A (ko) * 2017-03-10 2025-08-12 볼트 쓰레즈, 인크. 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
CA3072134A1 (en) 2017-05-31 2018-12-06 Universitat Fur Bodenkultur Wien Yeast expressing a synthetic calvin cycle
JP7717459B2 (ja) 2018-02-12 2025-08-04 ロンザ リミテッド 目的タンパク質を産生するための宿主細胞
CN113015782A (zh) 2018-11-19 2021-06-22 巴斯夫欧洲公司 用于酵母的前导序列
DK3874047T4 (da) 2019-01-11 2025-02-03 Lonza Ag Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle
AU2020252241B2 (en) 2019-04-01 2024-03-21 Universität Für Bodenkultur Wien Mut- methylotrophic yeast
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
CN110760003A (zh) * 2019-09-10 2020-02-07 广东药科大学 一种抗her3单链抗体的制备方法
EP4127185B1 (en) 2020-04-01 2025-06-04 Lonza Ltd. Helper factors for expressing proteins in yeast
CA3193155A1 (en) 2020-09-30 2022-04-07 Lonza Ltd Host cells overexpressing translational factors
WO2022144374A2 (en) 2020-12-29 2022-07-07 Bisy Gmbh Novel yeast strains
JP7756787B2 (ja) * 2021-07-30 2025-10-20 ヘライナ，インコーポレーテッド タンパク質合成および分泌のための方法および組成物
CN117794941A (zh) * 2021-07-30 2024-03-29 赫莱纳公司 用于蛋白质合成和分泌的方法和组合物
WO2024133538A1 (en) 2022-12-20 2024-06-27 Lonza Ltd Host cells with pat1 knockout for increased specific protein productivity
WO2024141641A2 (en) * 2022-12-30 2024-07-04 Biotalys NV Secretion signals
WO2026009000A1 (en) 2024-07-04 2026-01-08 Solasta Bio Limited Methods for production of insecticidal neuropeptides

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Publication number Priority date Publication date Assignee Title
WO1984001153A1 (en) 1982-09-15 1984-03-29 Collaborative Res Inc Production of interferon in yeast by use of invertase promoter
US5010182A (en) 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DE69133066T2 (de) 1990-01-16 2003-03-06 Centro De Ingenieria Genetica Y Biotecnologia, Cubanacan Playa Verfahren zur Expression heterologischer Gene in der Hefe Pichia Pastoris, Expressionsverfahren und transformierte Mikroorganismen
US5268273A (en) 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
DK82893D0 (da) 1993-07-08 1993-07-08 Novo Nordisk As Peptid
CN101679992A (zh) 2007-04-20 2010-03-24 波利门科学生物免疫研究有限公司 酵母菌表达系统
US7741075B2 (en) 2008-06-06 2010-06-22 Board Of Regents Of The University Of Nebraska Pichia pastoris PIR1 secretion signal peptide for recombinant protein expression and Pichia pastoris PIR1 and PIR2 anchor domain peptides for recombinant surface display
US8440456B2 (en) * 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
EP2258855A1 (en) * 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
DE102009055015B4 (de) 2009-12-18 2013-12-24 Technische Universität Dresden Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei
EP3590950A1 (en) 2011-05-09 2020-01-08 Ablynx NV Method for the production of immunoglobulin single varible domains
AU2013341049B2 (en) * 2012-10-29 2017-07-20 Lonza Ltd Expression sequences

Also Published As

Publication number Publication date
KR20150076160A (ko) 2015-07-06
SG11201502745XA (en) 2015-05-28
ES2789299T3 (es) 2020-10-26
JP2019141063A (ja) 2019-08-29
IL238420A0 (en) 2015-06-30
ES2610990T3 (es) 2017-05-04
HUE033282T2 (en) 2017-11-28
WO2014067926A1 (en) 2014-05-08
AU2013341049B2 (en) 2017-07-20
IL238420B (en) 2019-05-30
US20180135092A1 (en) 2018-05-17
AU2013341049A1 (en) 2015-04-02
DK2912053T3 (en) 2017-01-30
JP6556625B2 (ja) 2019-08-07
CA2886460A1 (en) 2014-05-08
CN104837857B (zh) 2018-11-06
US11359223B2 (en) 2022-06-14
CA3212565A1 (en) 2014-05-08
JP2015533286A (ja) 2015-11-24
BR112015008289A2 (pt) 2017-11-28
EP3130598A3 (en) 2017-05-03
IL266700B (en) 2021-08-31
US20150232904A1 (en) 2015-08-20
IN2015DN02771A (https=) 2015-09-04
CN104837857A (zh) 2015-08-12
PL2912053T3 (pl) 2017-05-31
EP3130598A2 (en) 2017-02-15
HK1208037A1 (en) 2016-02-19
EP2912053A1 (en) 2015-09-02
EP2912053B1 (en) 2016-11-09
IL266700A (en) 2019-07-31
CA2886460C (en) 2023-11-14
EP3130598B1 (en) 2020-02-12
US10160988B2 (en) 2018-12-25

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