KR102066474B1 - Complex microbial culture and fertilizer manufacturing method using the same - Google Patents

Abstract

The present invention relates to a method for manufacturing a complex microorganism cultivated body and a method for manufacturing fertilizer by using the complex microorganism cultivated body. According to the present invention, the method for manufacturing a complex microorganism cultivated body comprises: a step of mixing red soil, a mineral material, rice bran, and unpolished rice to a fermented juice which is made by fermenting complex juice acquired from pineapple, papaya, and persimmon at a constant temperature; a step of mixing the fermented juice with fossil shell powder and unrefined sugar and fermenting the mixture at a constant temperature to cultivate microorganisms; and a step of drying and pulverizing the mixture to manufacture a complex microorganism cultivated body. Accordingly, the complex microorganism cultivated body can provide sufficient effect in various variables of a natural state and can be provided as the most suitable fertilizer to environmentally-friendly and organic agricultural methods. Moreover, a fertilizer using the complex microorganism cultivated body simultaneously provides effectiveness and economic efficiency suitable for blight prevention and removal of crops, immunity increase and texture enhancement of livestock, neutralization of soil polluted by salt accumulation, heavy metal, and pesticide residue, fertility restoration of agricultural land where repeated cultivation hindrance occurs, and manufacture of an organic fertilizer and phosphoric acid (P)-potassium (K) fertilizer.

Description

Complex microbial culture and fertilizer manufacturing method using the same}

The present invention relates to a method for producing a complex microbial culture and a fertilizer production method using the complex microbial culture, and specifically to a method for producing a fertilizer, such as a soil improving agent and an organic fertilizer, which is used for cultivating a complex microorganism and the same. It is about.

In modern agriculture, chemical fertilizers and pesticides have been intensively used to increase the yield of crops. The use of suitable fertilizers leads to rapid growth of crops and the resulting increase in yield, but due to excessive fertilization, acidification of the soil of the farmland In order to overcome this problem, salt damage caused by salt accumulation and salt concentration caused by lack of organic matter in soil is repeated, and organic fertilizer containing microorganisms is used instead of chemical fertilizer and pesticide. There have been many attempts to solve biologically by microorganisms.

In the prior art related to the production of microorganism-containing fertilizers, Patent Document 1, which is mixed with crushed fruits, vegetables, flowers, herbs, vegetation at a certain ratio and the primary fermentation in the fermentation chamber and the primary fermented products food waste, livestock Organic fermentation products using soybeans composed of feces, fish and fish wastes by mixing the filtered filtrate with a certain ratio and fermentation in secondary proportions, and by supplementing the secondary fermentation with auxiliaries such as lime, ganban stone, kaolin and leadstone. Compound fertilizers are disclosed, and Patent Document 2 discloses a well mixed of 0.1 to 0.5% by weight of soil microorganisms and 99.5 to 99.9% by weight of organic waste such as rice bran and bean curd, and then put into a high-temperature fermenter at 20 to 30 at 60 to 200 ° C. After the first fermentation of the soil microbial group to prepare the fermented soil microorganism group 0.01 ~ 0.05% by weight and the organic fertilizer raw material 99.95 ~ 99.99% by weight of the highly concentrated nutrients, high heat fermenter It discloses an organic fertilizer containing soil microorganisms, characterized in that it is prepared by secondary fermentation at 100 ~ 200 ℃ 20 to 50 hours, the use of livestock meal is not preferable because there may be antibiotic contamination, such Soil microorganisms are insufficient to show various functions such as fertilizer and soil improvement.

In addition, Patent Document 3, Lactobacillus casei AMT-66 (KCTC 18441P) strain in a fertilizer raw material consisting of 60 to 90% by weight of axial fraction and at least 10 to 40% by weight of one or more carriers selected from the group consisting of sawdust, rice bran, rice straw, activated carbon and zeolite. It discloses a microbial fertilizer, characterized in that it is prepared by mixing fermentation, but may be insufficient to exhibit a variety of functions, such as fertilizer and soil improvement by the use of a single microorganism.

Although the above-mentioned prior art is a technique for producing a fertilizer containing microorganisms, organic fertilizers containing microorganisms are contaminated by various causes, exhibit high activity in soils where various indigenous microorganisms survive, or promote growth of various kinds of crops. Did not have a special effect.

KR 10-0238900 B KR 10-1017576 B KR 10-1836426 B

The problem to be solved by the present invention is to provide a method for producing a complex microorganism culture and a method for producing a fertilizer, such as soil improver and organic fertilizer used for the cultivation of crops using the compound microorganism.

More specifically, a method for producing a complex microbial culture that can be applied to agricultural land where salt pests and chemical fertilizers are excessively used for a long period of time, salt accumulation, injuries, and various diseases of crops are continuously produced, and a complex microbial culture obtained therefrom is used. It is an object of the present invention to provide a fertilizer manufacturing method.

The method for producing a composite microbial culture as a means of solving the problem according to the present invention is to mix the red soil, minerals, rice bran and brown rice in the fruit fermentation broth fermented at a certain temperature the fruit juice obtained from pineapple, papaya and persimmon, and remove the salt therein A fermentation mixture comprising crushed stone powder and unrefined brown sugar is fermented at a predetermined temperature to culture microorganisms, and then dried and pulverized to produce a composite microorganism culture.

One embodiment according to the method for producing a composite microbial culture of the present invention is a). A first step of obtaining a complex juice from a fruit consisting of 35% by weight of 2/3 ripe pineapple, 35% by weight papaya and 30% by weight persimmon, b). After fermenting the complex juice of the first step for 3 to 5 days at 25 ~ 40 ℃, aged for 5 hours at 60 ℃, and then separating and removing the solid content from the juice fermentation broth fermented again at 25 ~ 40 ℃ for 15 to 25 days , A second step of obtaining a complex microbial extract, c). The primary culture for 5 days while gradually raising the temperature from room temperature to 45 ℃ the fermentation mixture containing red clay, masato, rice bran, brown rice, salt-free calcite powder and unrefined brown sugar to the composite microbial extract obtained in the second step process And again, the second step of culturing for 5 days while gradually increasing the temperature to 60 ℃, and the third step step of obtaining a complex microorganism culture by tertiary culture for 5 days while gradually increasing the temperature to 80 ℃ and d). It comprises a fourth step of producing a complex microbial culture powder by drying and grinding the complex microbial culture obtained in the third step.

Fertilizer production method using a complex microbial culture as a means of solving another problem according to the present invention a) according to the present invention. First step to d). The microbial culture prepared by the fourth step process is combined with rice bran and maintained for one week to produce a composite microbial culture-containing fertilizer.

Another embodiment of the fertilizer production method using a composite microorganism culture according to the present invention is a) according to the present invention. First step to d). Comprising a compound microbial culture prepared by the fourth step process soluble phosphorus fertilizer, yonggurin fertilizer or potassium chloride fertilizer is prepared to produce a fertilizer containing the microorganism culture.

Another embodiment of the fertilizer production method using a composite microorganism culture according to the present invention is a) according to the present invention. First step to d). The microbial culture prepared by the fourth step is mixed with rice bran, compost or mixed well with various constituents to keep the moisture at 60 to 70% to produce a complex microbial culture-containing fertilizer.

Soluble phosphorus fertilizer, yonggurin fertilizer or potassium chloride fertilizer according to the present invention is selected from commercially available fertilizer, the compost is a compost produced from sawdust, rice straw, barley, deciduous leaves, etc., various kinds of nutrient is selected from manure, pig flour and manure Consists of one or more components

The composite microbial culture according to the present invention may exhibit sufficient effects even in various variables in the natural state, and may be provided as the most suitable fertilizer of eco-friendly farming and organic farming.

In addition, the fertilizer containing the composite microorganism culture according to the present invention is to prevent and solve the disease occurrence of crops, neutralize the soil contaminated with salt accumulation and heavy metals and residual pesticides, the recovery of the intellect of the agricultural land where the crop failure, organic fertilizer and P ( Phosphoric acid) -K (cali) It has the advantage of having both the efficacy and economics suitable for the manufacture of fertilizers.

In addition, using the microorganism culture according to the present invention as a livestock feed exhibits the effect of increasing the immunity and meat quality of the livestock.

Below Although the present invention will be described in more detail through specific contents, examples (manufacture examples) and test examples for carrying out the present invention, the present invention is not limited by the following description.

Method for producing a composite microbial culture of the present invention is a). A first step of obtaining a complex juice from a fruit consisting of 35% by weight of 2/3 ripe pineapple, 35% by weight papaya and 30% by weight persimmon, b). After fermenting the complex juice of the first step for 3 to 5 days at 25 ~ 40 ℃, aged for 5 hours at 60 ℃, and then separating and removing the solid content from the juice fermentation broth fermented again at 25 ~ 40 ℃ for 15 to 25 days , A second step of obtaining a complex microbial extract, c). The primary culture for 5 days while gradually raising the temperature from room temperature to 45 ℃ the fermentation mixture containing red clay, masato, rice bran, brown rice, salt-free calcite powder and unrefined brown sugar to the composite microbial extract obtained in the second step process After the second step of culturing to 60 ℃ by the heat of fermentation while maintaining for 4 to 5 days, and then the third step to obtain a composite microorganism culture by three-day culturing for 5 days while gradually increasing the temperature to 80 ℃ and d) . It comprises a fourth step of producing a complex microbial culture powder by drying and grinding the complex microbial culture obtained in the third step.

The first step according to the present invention is to obtain a complex juice from the fruit consisting of 2/3 ripe pineapple, papaya and persimmon pineapple 35% by weight, papaya 35% by weight and persimmon 30% using a conventional juicer, etc. It consists of mixing in%, and extracting juice from this, and it is also possible to mix the juice obtained by juice from each fruit.

The second step process according to the invention is a process of obtaining a complex microbial extract after fermenting the complex juice of pineapple, papaya and persimmon obtained in the first step process, the complex juice of the first step process at 25 ~ 40 ℃ After fermentation for 3 to 5 days and aged for 5 hours at 60 ℃, and then separated and removed from the fruit fermentation broth fermented at 25 to 40 ℃ for 15 to 25 days, it consists of obtaining a complex microbial extract.

The third step process according to the present invention is a process of fermenting a fermentation compound containing red clay, masato, rice bran, brown rice, crushed fossil powder and unrefined brown sugar in the complex microbial extract obtained in the second step. Extract: Red soil: Masato: Rice bran: Brown rice: Unsalted crushed powder: Unrefined brown sugar by weight ratio 1: 25000 to 32000: 18000 to 22000: 2800 to 3200: 6800 to 7200: 9600 to 10400: 960 to 1040 It consists of doing

In the fermentation mixture, red soil: masato is preferably combined at a weight ratio of 6: 4, and rice bran: brown rice is preferably combined at a weight ratio of 3: 7. In addition, the brown sugar, which is not purified, is preferably blended in 10% by weight of the weight of the crushed crushed stone, and consists of fermentation three times under conditions of 60 to 65% by weight of water.

The red soil and Masato have a particle size of 0.02 ~ 0.05㎜, red soil is a geological region to create microorganisms, Masato is a porous mineral to create an environment in which microorganisms can proliferate well by smoothly supplying oxygen Since it contains air in the pores, it acts as an oxygen source during the fermentation process, and the calcite powder from which the salt is removed is commercially available by crushing the oyster shell from which the salt is removed by heating to a high temperature of 700 ° C. or higher, and the fermentation of microorganisms is easy. It acts as an oxygen generator for activation.

In the third fermentation process of the third step according to the present invention, the primary fermentation compound is primarily cultured for 1 day while gradually increasing the temperature from room temperature to 45 ° C., using fermentation heat generated during the fermentation process. The fermentation is carried out for 2 to 5 days until the fermentation temperature reaches 60 ℃. Thereafter, the mixture is fermented for 5 days while gradually raising the temperature to 80 ° C. by heating to obtain a composite microbial culture.

The fourth step process according to the present invention consists of drying the complex microorganism culture obtained in the third step process at 25 ℃ for 5 days, and then finely milled to produce a complex microorganism culture.

The complex microbial culture according to the present invention can be fertilized by spraying the foliar or root of the crop in the form of a liquid fertilizer mixed with water or unsweetened brown sugar.

The liquid fertilizer form of the composite microbial culture 6kg + brown sugar 4kg + 1.2L of water according to the present invention, and uniformly mixed, and then allowed to stand for 24 hours, and then added 500L of water again, and prepared by standing for 24 hours The liquid fertilizer is used as it is or fertilized on the foliar of the crop, or the liquid fertilizer prepared in the above ratio is again mixed with 3500 L of water to fertilize the root of the crop.

When fertilizing the liquid fertilizer according to the present invention on the foliar of the crop, the leaf surface promotes the nutrient supply activity of the microorganisms, the leaves are coveted, glossy, dark blue appear, accelerate the growth and harvest of fruit, There is an effect of increasing the marketability, and when applied to the roots, it is possible to suppress the occurrence of the crops of the crops, and to lower the roots of the crops.

And one embodiment of the fertilizer manufacturing method using a composite microorganism culture according to the present invention is a) of the present invention. First step to d). 4 to 5 parts by weight of unrefined brown sugar or unrefined brown sugar is added to 1 part by weight of the microorganism culture prepared by the fourth step, and then 35 to 40 parts by weight of chemical fertilizer containing soluble fertilizer or yongguarin and potassium chloride fertilizer. , The chemical fertilizer consists of preparing a composite microbial culture-containing phosphate-soluble fertilizer is composed of 0.3 parts by weight to 25 parts by weight of potassium chloride fertilizer with respect to 10 parts by weight of soluble phosphorus fertilizer or yongguarin.

It is preferable to mix 1 to 1.2 parts by weight of brown sugar, which is not purified in the preparation of the phosphate-soluble fertilizer, with respect to 1 part by weight of the microorganism culture, and the ratio of the soluble phosphorus ratio or the mixing ratio of yonggurin and potassium chloride fertilizer according to the rapid growth and inhibition of growth of plants. It is possible to fertilize in the above range.

Another embodiment of the fertilizer production method using a composite microorganism culture according to the present invention is a) of the present invention. First step to d). 15 parts by weight to 100 parts by weight of rice bran per 1 part by weight of the mixed microorganism culture prepared by the fourth step and maintained for 24 hours, then mixed with 300 to 500 parts by weight of compost and covered with a light shielding film under 60% by weight of moisture It consists of preparing organic fertilizer containing the microorganism culture by maintaining in 4-5 days in the furnace.

Preparation of the composite microorganism-containing organic fertilizer is that the production is completed when the temperature rises to 70 ~ 80 ℃ by self-heat generated during the ripening process for 4 to 5 days in the shade in the state covered with a light shielding film after compounding compost To use as a fertilizer.

The compost is a compost made by blending at least one component from food waste, fibrous material and inorganic ingredients to one or more livestock meal selected from poultry meal, pig meal and cow manure, wherein the fibrous material is selected from one or more of sawdust, rice straw, barley and deciduous leaves. The mineral component consists of at least one of clay and oyster shell.

In addition, the microbial culture prepared according to the present invention contains probiotic strains that decompose harmful toxins in the intestine of the animal, so that it can be used as an additive in livestock feed, and the microbial culture is 0.2 to the total weight of the feed. Weight percent by weight.

For example, a livestock feed containing the complex microbial culture according to the present invention, after mixing the mixed microbial culture 2kg, rice bran 2kg ~ 50kg, stored for 7 days in a closed state in a dry shade, then feed 7 days It is mixed with 1 ton and used as livestock feed.

Hereinafter, the fertilizer manufacturing method using the complex microbial culture and the complex microbial culture according to the present invention will be described in more detail.

<Example 1>

 a). First step and second step (complex juice and complex microbial extract preparation)

  2/3 ripe pineapple, papaya and persimmons were prepared, and 35% by weight of pineapple, 35% by weight of papaya and 30% by weight of persimmon were juiced using a juicer to obtain a composite juice.

The complex juice was fermented at 35 ° C. for 5 days, aged at 60 ° C., and then fermented at 35 ° C. for 25 days to separate the solids by means of filtration or the like, thereby obtaining a complex microbial extract.

 b). 3rd and 4th steps (composite microbial culture and culture powder production)

In the prepared container, 1 kg of the mixed microorganism extract obtained above, 300 kg of red soil, 200 kg of masato, 200 kg of rice bran, 70 kg of brown rice, 10 kg of unrefined brown sugar, and 100 kg of demineralized crushed stone powder were added, mixed uniformly, and then water was added. The content was 65% by weight to obtain a fermentation mixture.

The fermentation mixture was left in the fermentation chamber, and after primary culture for 5 days until the temperature of the fermentation product was gradually raised to 45 ° C while gradually raising the temperature of the fermentation chamber, the heating was stopped, and the temperature of the fermentation product was increased to 60 ° C by the heat of fermentation itself. The fermentation was carried out while maintaining for 5 days until. Thereafter, the temperature of the fermentation chamber was increased again, and the mixture was maintained for 5 days while gradually increasing the temperature until the temperature of the fermentation product reached 80 ° C., thereby terminating the third fermentation, thereby obtaining a composite microbial culture.

The obtained microbial culture was dried for 5 days while maintaining at 25 ° C., and then the dried microbial culture was finely ground to prepare a composite microbial culture powder according to the present invention.

<Test Example>

In order to identify the microorganisms alive in the composite microorganism culture according to the present invention prepared in <Example 1> it was confirmed that it contains 22 kinds of microorganisms through genome sequencing (DNA sequencing) It is shown in [Table 1].

division microbe division microbe One Lactobacillus reuteri 12 Lactobacillus casei 2 Bifidobacterium bifidum 13 Lactobacillus bulgaricus 3 Bacillus subtillis 14 Lactobacillus lactis 4 Enterococcus faecium 15 Bacillus licheniformis 5 Lactobacillus plantarum 16 Saccharomyces cerevisiae 6 Lactobacillus acidophilus 17 Lactobacillus salivarius 7 Clostridium butyricum 18 Bacillus polyfermenticus 8 Lactobacillus brevis 19 Bacillus cereus 9 Lactobacillus fermentum 20 Pediococcus acidilactici 10 Bifidobacterium longum 21 Bifidobacterium thermophilum 11 Bacillus coagulans 22 Aspergillus oryzae

<Example 2> (Manufacture of phosphate-soluble fertilizer containing complex microorganism culture)

4 kg of the mixed microorganism culture medium prepared in <Example 1> and 5 kg of unrefined brown sugar were added to the prepared container, and then a mixture containing 130 kg of a soluble fertilizer or a long-acting fertilizer and 30 kg of potassium chloride fertilizer was uniformly mixed to contain phosphoric acid containing the complex microorganism culture. Soluble fertilizer was prepared.

The prepared microbial culture-containing phosphate-soluble fertilizer according to the present invention is water-soluble, so that phosphoric acid is accommodated in crops without accumulation of insoluble phosphoric acid and grown to high quality.

<Example 3> (Manufacture of organic fertilizer containing a hybrid microorganism culture)

After mixing 100 kg of rice bran uniformly with 10 L of water to 2 kg of the mixed microorganism culture prepared in <Example 1>, it is deposited to a uniform thickness in a shaded deposit and maintained for 24 hours.

After 24 hours, 1000 kg of conventional compost prepared with previously prepared livestock meal, food waste, rice straw, barley, and fallen leaves of fibrous material was blended, deposited at a uniform thickness, and then covered with a light shielding film for 5 days. When the temperature rises to 75 ° C, compost is spread out to complete the home cooking process and used as fertilizer.

The organic fertilizer containing the composite microorganism culture according to the present invention prepared above is reduced to 1/4 to 1/5 times the amount of fertilization as compared to the conventional composts that are housed in fibrous materials of livestock meal, food waste, rice straw, barley and deciduous leaves. This allows the crops to be grown more harvested without causing pests without contaminating the soil.

Claims (7)

a). The first step of obtaining a complex juice from a fruit consisting of 35% by weight of 2/3 ripe pineapple, 35% by weight papaya and 30% by weight persimmon,
b). After fermenting the complex juice of the first step 3 to 5 days at 25 ~ 40 ℃, aged for 5 hours at 60 ℃, and then separating and removing the solid content from the juice fermentation broth fermented again at 25 ~ 40 ℃ for 15 to 25 days , The second step of obtaining a complex microbial extract,
c). The primary culture for 5 days while gradually raising the temperature from room temperature to 45 ℃ the fermentation mixture containing red clay, masato, rice bran, brown rice, salt-free calcite powder and unrefined brown sugar to the composite microbial extract obtained in the second step process After the second step of culturing to 60 ℃ by the heat of fermentation, while maintaining for 4 to 5 days, then the third step of obtaining a complex microorganism culture by culturing three times for 5 days while gradually increasing the temperature to 80 ℃ and
d). And a fourth step of preparing a complex microbial culture powder by drying and pulverizing the complex microbial culture obtained in the third step.
delete 4 to 5 parts by weight of brown sugar or unrefined brown sugar is added to 1 part by weight of the microbial culture prepared according to the method of claim 1, followed by mixing 35 to 40 parts by weight of a chemical fertilizer containing a soluble fertilizer or a potion and a potassium chloride fertilizer. However, the chemical fertilizer is a fertilizer manufacturing method using a composite microorganism culture, characterized in that the composition is composed of 0.3 to 25 parts by weight of potassium chloride fertilizer based on 10 parts by weight of soluble phosphorus fertilizer or yongguarin. The method of claim 3, wherein the unrefined brown sugar is mixed with 1 to 1.2 parts by weight based on 1 part by weight of the microbial culture. 1 to 100 parts by weight of rice bran per 1 part by weight of the composite microorganism culture prepared by the method according to claim 1 was mixed and maintained for 24 hours, mixed with 300 to 500 parts by weight of compost, and covered with a light shielding film under 60 parts by weight of water. Fertilizer production method using a composite microorganism culture, characterized in that it is maintained in the shade for 4 to 5 days. The composite microorganism according to claim 5, wherein when the temperature is raised to 70 to 80 ° C. by self-heat generated during the ripening process when it is kept in the shade for 4 to 5 days after the compost is blended, the microorganism is completed. Fertilizer manufacturing method using a culture. 6 kg of the mixed microbial culture prepared by the method of claim 1, 4 kg of unrefined brown sugar, and 1.2 L of water are mixed and uniformly mixed, and then allowed to stand for 24 hours, and then 500 L of water is added again and left for 24 hours. Fertilizer production method using a composite microorganism culture.