KR102371464B1 - Method for producing fertilizer using egg shell and various material - Google Patents

Method for producing fertilizer using egg shell and various material Download PDF

Info

Publication number
KR102371464B1
KR102371464B1 KR1020210010370A KR20210010370A KR102371464B1 KR 102371464 B1 KR102371464 B1 KR 102371464B1 KR 1020210010370 A KR1020210010370 A KR 1020210010370A KR 20210010370 A KR20210010370 A KR 20210010370A KR 102371464 B1 KR102371464 B1 KR 102371464B1
Authority
KR
South Korea
Prior art keywords
weight
eggshell
fermented
fertilizer
shiitake
Prior art date
Application number
KR1020210010370A
Other languages
Korean (ko)
Inventor
김선숙
Original Assignee
농업회사법인 유한회사 서호
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 농업회사법인 유한회사 서호 filed Critical 농업회사법인 유한회사 서호
Priority to KR1020210010370A priority Critical patent/KR102371464B1/en
Application granted granted Critical
Publication of KR102371464B1 publication Critical patent/KR102371464B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Botany (AREA)
  • Mechanical Engineering (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to a method for manufacturing an eggshell mixed fertilizer capable of producing high-quality crops by using fermented eggshell products and various materials, and to the eggshell mixed fertilizer manufactured by the method. According to the present invention, a high value-added fertilizer can be produced using mainly discarded eggshells, and the fertilizer of the present invention manufactured by combining fermented eggshells with various materials has an effect of producing high-quality crops.

Description

계란 껍질과 다양한 소재를 이용한 비료의 제조방법{Method for producing fertilizer using egg shell and various material}Method for producing fertilizer using egg shell and various materials {Method for producing fertilizer using egg shell and various material}

본 발명은 (1) 계란껍질, 물, 락토스 및 나한과 분말을 혼합한 난각 혼합물에 미생물 배양액을 접종한 후 발효시킨 난각 발효물을 건조한 후 분쇄하는 단계; (2) 표고버섯 균사체, 물, 락토스, 비파잎 분말, 쌀겨 분말 및 올리브잎 분말을 혼합한 표고 혼합물을 배양하고 건조하여 표고버섯 발효물을 제조하는 단계; 및 (3) 계분과 상기 (1)단계의 분쇄한 난각 발효물 및 상기 (2)단계의 제조한 표고버섯 발효물을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 난각 비료의 제조방법 및 상기 방법으로 제조된 난각 비료에 관한 것이다.The present invention comprises the steps of: (1) inoculating a microbial culture solution into an eggshell mixture of eggshell, water, lactose, and powder with eggshell, drying and grinding the fermented eggshell; (2) culturing and drying a shiitake mixture obtained by mixing shiitake mushroom mycelium, water, lactose, loquat leaf powder, rice bran powder and olive leaf powder to prepare a fermented shiitake mushroom; and (3) mixing chicken meal with the pulverized egg shell fermented product in step (1) and the fermented shiitake mushroom prepared in step (2). It relates to an eggshell fertilizer produced by the method.

종래의 농작물 재배 방식은 순수한 토양에서 재배하는 것이 관행으로 이어졌는데, 이러한 재배 방식은 뿌리의 산소공급이 부족하고 온도 및 일조량과 같은 변화요인으로 작황이 불안정하여, 카오린(kaolin)을 주성분으로 하는 상토(上土)로 대체하고 있으나, 이러한 토양에는 무기양분이 결핍되기 쉽기 때문에 영양보충을 위해 별도의 질소(N), 인산(H3PO4), 칼륨(K) 같은 무기영양분을 주요 성분으로 하는 복합비료의 일종인 화학비료를 공급하는 실정이다.The conventional cultivation method of crops has led to the practice of cultivation in pure soil. In this cultivation method, the oxygen supply to the roots is insufficient and the crop is unstable due to change factors such as temperature and sunlight. (上土), but these soils are prone to lack of inorganic nutrients, so for nutritional supplementation, separate inorganic nutrients such as nitrogen (N), phosphoric acid (H 3 PO 4 ), and potassium (K) are used as main components. Chemical fertilizers, which are a kind of compound fertilizers, are currently being supplied.

초창기에는 퇴비를 비료로 사용하였으나, 퇴비는 퇴비중의 75%나 되는 수분을 동시에 처리하는 것이므로 그 처리무게가 상당하여 노동력 소비가 많은 비효율적인 단점을 가지고 있기 때문에, 오늘날에는 천연 유기질 비료에 비해 소량으로도 충분하며, 또 운반이나 저장이 쉽고, 악취나 더럽혀지는 일이 없고 기생 생물이 생길 염려도 없는 화학비료가 주로 이용되고 있는 것이다. 이 화학비료는 무기염 또는 쉽게 무기염 형태가 되기 때문에 이온의 형태로 물에 쉽게 녹을 수 있어, 토양에 준 화학비료는 토양입자에 흡착되어 유지된다.In the early days, compost was used as a fertilizer, but since compost treats 75% of the compost at the same time, the processing weight is considerable and it consumes a lot of labor. Chemical fertilizers that are sufficient, easy to transport and store, do not cause odor or soiling, and do not cause parasites are mainly used. Since this chemical fertilizer is in the form of an inorganic salt or an inorganic salt easily, it can easily dissolve in water in the form of ions.

그러나, 화학비료는 질산암모늄(NH4NO3), 황산칼륨(K2SO4) 속의 황산 이온이나 염화암모늄(NH4Cl), 염화칼륨(KCl)속의 염소 이온처럼 비료성분 이외의 토질을 산성화시키는 이온도 다량 함유하고 있고, 속효성을 띠기 때문에, 다량으로 사용하게 되면 오히려 작물의 생육을 저하하게 된다.However, chemical fertilizers, like sulfate ions in ammonium nitrate (NH 4 NO 3 ) and potassium sulfate (K 2 SO 4 ), or chlorine ions in ammonium chloride (NH 4 Cl) and potassium chloride (KCl), acidify the soil other than fertilizer components. Since it contains a large amount of ions and has a fast-acting effect, if used in a large amount, the growth of crops is rather reduced.

한국등록특허 제1977171호에는 유기성 퇴비 비료의 생산방법이 개시되어 있고, 한국등록특허 제2042331호에는 한약부산물과 접착재료로 조성되는 둥근 입자비료의 제조방법이 개시되어 있으나, 본 발명의 계란 껍질과 다양한 소재를 이용한 이용한 비료의 제조방법과는 상이하다.Korea Patent No. 1977171 discloses a method for producing organic compost fertilizer, and Korean Patent No. 2042331 discloses a method for producing round particle fertilizer composed of herbal medicinal by-products and adhesive materials. It is different from the manufacturing method of fertilizer using various materials.

본 발명은 상기와 같은 요구에 의해 안출된 것으로서, 본 발명의 목적은 주로 버려지는 계란껍질과 다양한 소재를 이용하여 비료를 제조하기 위해, 게란껍질 전처리, 부재료 선정, 발효조건 등의 제조조건을 최적화하여, 고품질의 작물을 생산할 수 있는 비료의 제조방법을 제공하는 데 있다.The present invention has been devised in response to the above needs, and an object of the present invention is to optimize manufacturing conditions such as eggshell pretreatment, auxiliary material selection, and fermentation conditions in order to manufacture fertilizer using mainly discarded eggshells and various materials. Accordingly, to provide a method for manufacturing a fertilizer capable of producing high-quality crops.

상기 과제를 해결하기 위해, 본 발명은 (1) 계란껍질, 물, 락토스 및 나한과 분말을 혼합한 난각 혼합물에 미생물 배양액을 접종한 후 발효시킨 난각 발효물을 건조한 후 분쇄하는 단계; (2) 표고버섯 균사체, 물, 락토스, 비파잎 분말, 쌀겨 분말 및 올리브잎 분말을 혼합한 표고 혼합물을 배양하고 건조하여 표고버섯 발효물을 제조하는 단계; 및 (3) 계분과 상기 (1)단계의 분쇄한 난각 발효물 및 상기 (2)단계의 제조한 표고버섯 발효물을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 난각 비료의 제조방법을 제공한다.In order to solve the above problems, the present invention provides the steps of: (1) inoculating a microbial culture solution into an eggshell mixture mixed with eggshell, water, lactose, and powdery mildew, drying the fermented eggshell product, and pulverizing the fermented eggshell; (2) culturing and drying a shiitake mixture obtained by mixing shiitake mushroom mycelium, water, lactose, loquat leaf powder, rice bran powder and olive leaf powder to prepare a fermented shiitake mushroom; and (3) mixing chicken meal with the pulverized egg shell fermented product of step (1) and the fermented shiitake mushroom prepared in step (2). do.

또한, 본 발명은 상기 방법으로 제조된 난각 비료를 제공한다.In addition, the present invention provides an egg shell fertilizer prepared by the above method.

본 발명은 주로 버려지는 난각을 이용하여 고부가가치의 비료로 제조할 수 있으며, 난각 발효물과 다양한 소재를 조합하여 제조한 본 발명의 비료는 고품질의 작물을 생산할 수 있는 효과가 있다.According to the present invention, high value-added fertilizers can be manufactured using mainly discarded eggshells, and the fertilizers of the present invention prepared by combining fermented eggshells with various materials have the effect of producing high-quality crops.

본 발명의 목적을 달성하기 위하여, 본 발명은In order to achieve the object of the present invention, the present invention

(1) 계란껍질, 물, 락토스 및 나한과 분말을 혼합한 난각 혼합물에 미생물 배양액을 접종한 후 발효시킨 난각 발효물을 건조한 후 분쇄하는 단계;(1) inoculating a microbial culture solution into an eggshell mixture of eggshell, water, lactose, and powder with eggshell, drying and pulverizing the fermented eggshell;

(2) 표고버섯 균사체, 물, 락토스, 비파잎 분말, 쌀겨 분말 및 올리브잎 분말을 혼합한 표고 혼합물을 배양하고 건조하여 표고버섯 발효물을 제조하는 단계; 및(2) culturing and drying a shiitake mixture obtained by mixing shiitake mushroom mycelium, water, lactose, loquat leaf powder, rice bran powder and olive leaf powder to prepare a fermented shiitake mushroom; and

(3) 계분과 상기 (1)단계의 분쇄한 난각 발효물 및 상기 (2)단계의 제조한 표고버섯 발효물을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 난각 비료의 제조방법을 제공한다.(3) provides a method for producing egg shell fertilizer, comprising mixing chicken meal with the pulverized egg shell fermented product of step (1) and the fermented shiitake mushroom prepared from step (2) .

본 발명의 난각 비료의 제조방법에서, 상기 (1)단계는 바람직하게는 난각 혼합물 총 중량 기준으로, 계란껍질 45~55 중량%, 물 27~33 중량%, 락토스 10~14 중량% 및 나한과 분말 6~10 중량%를 혼합한 난각 혼합물에 미생물 배양액을 접종한 후 35~45℃에서 40~50일 동안 발효시킨 난각 발효물을 건조한 후 분쇄할 수 있으며, 더욱 바람직하게는 계란껍질 50 중량%, 물 30 중량%, 락토스 12 중량% 및 나한과 분말 8 중량%를 혼합한 난각 혼합물에 미생물 배양액을 접종한 후 40℃에서 45일 동안 발효시킨 난각 발효물을 건조한 후 분쇄할 수 있다.In the method for producing eggshell fertilizer of the present invention, step (1) preferably comprises 45 to 55% by weight of eggshell, 27 to 33% by weight of water, 10 to 14% by weight of lactose, and Nahan, based on the total weight of the eggshell mixture. After inoculating the microbial culture solution into the egg shell mixture mixed with 6-10 wt% of powder, the fermented eggshell fermented at 35-45 ° C. for 40-50 days can be dried and pulverized, more preferably eggshell 50 wt% , 30% by weight of water, 12% by weight of lactose, and 8% by weight of Nahan and powder 8% by weight of eggshell mixture is inoculated with a microbial culture, and then the fermented eggshell fermented at 40°C for 45 days can be dried and pulverized.

또한, 본 발명의 난각 비료의 제조방법에서, 상기 (1)단계의 미생물은 바실러스 서브틸리스(Bacillus subtilis), 락토바실러스 파라파라기니스(Lactobacillus parafarraginis), 락토바실러스 람노서스(Lactobacillus rhamnosus), 락토바실러스 플란타룸(Lactobacillus plantarum) 및 사카로마이세스 세레비지애(Saccharomyces cerevisiae)로 이루어진 군으로부터 선택되는 하나 이상의 미생물일 수 있으나, 이에 제한되지 않는다.In addition, in the method for producing eggshell fertilizer of the present invention, the microorganisms of step (1) are Bacillus subtilis , Lactobacillus parafarraginis , Lactobacillus rhamnosus , Lactobacillus Plantarum ( Lactobacillus plantarum ) and Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) It may be one or more microorganisms selected from the group consisting of, but is not limited thereto.

또한, 본 발명의 난각 비료의 제조방법에서, 상기 (2)단계의 표고버섯 발효물은 바람직하게는 표고 혼합물 총 중량 기준으로, 표고버섯 균사체 35~45 중량%, 물 13~17 중량%, 락토스 4~6 중량%, 비파잎 분말 8~12 중량%, 쌀겨 분말 18~22 중량% 및 올리브잎 분말 8~12 중량%를 혼합한 표고 혼합물을 34~40℃에서 6~8일 동안 배양하고 35~45℃에서 20~28시간 동안 건조하여 제조할 수 있으며, 더욱 바람직하게는 표고 혼합물 총 중량 기준으로, 표고버섯 균사체 40 중량%, 물 15 중량%, 락토스 5 중량%, 비파잎 분말 10 중량%, 쌀겨 분말 20 중량% 및 올리브잎 분말 10 중량%를 혼합한 표고 혼합물을 37℃에서 7일 동안 배양하고 40℃에서 24시간 동안 건조하여 제조할 수 있다.In addition, in the method for producing egg shell fertilizer of the present invention, the fermented shiitake mushroom in step (2) is preferably based on the total weight of the shiitake mixture, 35 to 45% by weight of shiitake mycelium, 13 to 17% by weight of water, and lactose A shiitake mixture of 4 to 6% by weight, 8 to 12% by weight of loquat leaf powder, 18 to 22% by weight of rice bran powder, and 8 to 12% by weight of olive leaf powder was cultured at 34-40℃ for 6-8 days, 35 It can be prepared by drying at ~45°C for 20 to 28 hours, and more preferably, based on the total weight of the shiitake mixture, 40% by weight of shiitake mycelium, 15% by weight of water, 5% by weight of lactose, 10% by weight of loquat leaf powder , it can be prepared by culturing a shiitake mixture in which 20% by weight of rice bran powder and 10% by weight of olive leaf powder are mixed at 37°C for 7 days and dried at 40°C for 24 hours.

또한, 본 발명의 난각 비료의 제조방법에서, 상기 (3)단계의 혼합은 바람직하게는 난각 비료 총 중량 기준으로, 계분 60 중량%, 난각 발효물 20 중량% 및 표고버섯 발효물 20 중량%를 혼합할 수 있다. 상기와 같은 조건으로 혼합하는 것이 고품질의 작물 생산에 적합한 혼합 비료로 제조할 수 있었다.In addition, in the method for producing an eggshell fertilizer of the present invention, the mixing in step (3) is preferably based on the total weight of the eggshell fertilizer, 60% by weight of chicken manure, 20% by weight of fermented eggshell and 20% by weight of fermented shiitake can be mixed. Mixing under the same conditions as described above was able to produce a mixed fertilizer suitable for the production of high-quality crops.

본 발명의 난각 비료의 제조방법은, 보다 구체적으로는The manufacturing method of the egg shell fertilizer of the present invention is more specifically

(1) 난각 혼합물 총 중량 기준으로, 계란껍질 45~55 중량%, 물 27~33 중량%, 락토스 10~14 중량% 및 나한과 분말 6~10 중량%를 혼합한 난각 혼합물에 미생물 배양액을 접종한 후 35~45℃에서 40~50일 동안 발효시킨 난각 발효물을 건조한 후 분쇄하는 단계;(1) Based on the total weight of the eggshell mixture, inoculate a microbial culture solution into an eggshell mixture containing 45 to 55% by weight of eggshell, 27 to 33% by weight of water, 10 to 14% by weight of lactose, and 6 to 10% by weight of Nahan and powder drying and pulverizing the fermented egg shells fermented for 40-50 days at 35-45° C.;

(2) 표고 혼합물 총 중량 기준으로, 표고버섯 균사체 35~45 중량%, 물 13~17 중량%, 락토스 4~6 중량%, 비파잎 분말 8~12 중량%, 쌀겨 분말 18~22 중량% 및 올리브잎 분말 8~12 중량%를 혼합한 표고 혼합물을 34~40℃에서 6~8일 동안 배양하고 35~45℃에서 20~28시간 동안 건조하여 표고버섯 발효물을 제조하는 단계; 및(2) based on the total weight of the shiitake mixture, 35 to 45% by weight of shiitake mycelium, 13 to 17% by weight of water, 4 to 6% by weight of lactose, 8 to 12% by weight of loquat leaf powder, 18 to 22% by weight of rice bran powder, and Preparing a fermented shiitake mushroom by culturing the shiitake mixture mixed with 8-12 wt% of olive leaf powder at 34-40 ° C. for 6-8 days and drying at 35-45 ° C. for 20-28 hours; and

(3) 난각 비료 총 중량 기준으로, 계분 60 중량%와 상기 (1)단계의 분쇄한 난각 발효물 20 중량% 및 상기 (2)단계의 제조한 표고버섯 발효물 20 중량%를 혼합하는 단계를 포함할 수 있으며,(3) Based on the total weight of the eggshell fertilizer, mixing 60% by weight of chicken manure, 20% by weight of the pulverized eggshell fermented product of step (1), and 20% by weight of the fermented shiitake mushroom prepared in step (2). may include,

더욱 구체적으로는more specifically

(1) 난각 혼합물 총 중량 기준으로, 계란껍질 50 중량%, 물 30 중량%, 락토스 12 중량% 및 나한과 분말 8 중량%를 혼합한 난각 혼합물에 미생물 배양액을 접종한 후 40℃에서 45일 동안 발효시킨 난각 발효물을 건조한 후 분쇄하는 단계;(1) Based on the total weight of the eggshell mixture, 50% by weight of eggshell, 30% by weight of water, 12% by weight of lactose, and 8% by weight of eggshell powder and eggshell mixture After inoculation of a microorganism culture medium at 40℃ for 45 days drying and pulverizing the fermented egg shell product;

(2) 표고 혼합물 총 중량 기준으로, 표고버섯 균사체 40 중량%, 물 15 중량%, 락토스 5 중량%, 비파잎 분말 10 중량%, 쌀겨 분말 20 중량% 및 올리브잎 분말 10 중량%를 혼합한 표고 혼합물을 37℃에서 7일 동안 배양하고 40℃에서 24시간 동안 건조하여 표고버섯 발효물을 제조하는 단계; 및(2) Shiitake mixed with 40 wt% of shiitake mycelium, 15 wt% of water, 5 wt% of lactose, 10 wt% of loquat leaf powder, 20 wt% of rice bran powder and 10 wt% of olive leaf powder based on the total weight of the shiitake mixture culturing the mixture at 37° C. for 7 days and drying it at 40° C. for 24 hours to prepare a fermented shiitake; and

(3) 난각 비료 총 중량 기준으로, 계분 60 중량%와 상기 (1)단계의 분쇄한 난각 발효물 20 중량% 및 상기 (2)단계의 제조한 표고버섯 발효물 20 중량%를 혼합하는 단계를 포함할 수 있다.(3) Based on the total weight of the eggshell fertilizer, mixing 60% by weight of chicken manure, 20% by weight of the pulverized eggshell fermented product of step (1), and 20% by weight of the fermented shiitake mushroom prepared in step (2). may include

본 발명은 또한, 상기 한 항의 방법으로 제조된 난각 비료를 제공한다.The present invention also provides an egg shell fertilizer prepared by the method of the preceding paragraph.

이하, 본 발명의 실시예 및 실험예를 들어 상세히 설명한다. 단, 하기 실시예 및 실험예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예 및 실험예에 한정되는 것은 아니다.Hereinafter, examples and experimental examples of the present invention will be described in detail. However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples and Experimental Examples.

실시예 1. 계란껍질을 이용한 비료Example 1. Fertilizer using eggshell

(1) 난각 혼합물 총 중량 기준으로, 분쇄한 계란껍질 50 중량%에 정제수 30 중량%, 락토스 12 중량% 및 나한과 분말(오붐주식회사) 8 중량%를 혼합한 난각 혼합물에 각각의 미생물의 농도가 105 cfu/㎖씩 포함된 유용 미생물 배양액(Bacillus subtilis, Lactobacillus parafarraginis, Lactobacillus rhamnosus, Lactobacillus plantarum 및 Saccharomyces cerevisiae) 5%(v/w) 접종한 후 40℃에서 45일 동안 교반하면서 발효시킨 난각 발효물을 40℃에서 24시간 동안 건조한 후 분쇄하였다.(1) Based on the total weight of the eggshell mixture, the concentration of each microorganism was found in the eggshell mixture of 50% by weight of crushed eggshell, 30% by weight of purified water, 12% by weight of lactose, and 8% by weight of Nahan and powder (Oboom Co., Ltd.) Fermented egg shells were inoculated with 5% (v/w) of useful microorganism culture ( Bacillus subtilis , Lactobacillus parafarraginis, Lactobacillus rhamnosus, Lactobacillus plantarum, and Saccharomyces cerevisiae ) containing 10 5 cfu/ml and fermented at 40°C for 45 days with stirring. was dried at 40° C. for 24 hours and then pulverized.

(2) 표고 혼합물 총 중량 기준으로, 표고버섯 균사체 40 중량%에 정제수 15 중량%, 락토스 5 중량%, 비파잎 분말 10 중량%, 쌀겨 분말 20 중량% 및 올리브잎 분말 10 중량%를 혼합한 표고 혼합물을 34~40℃에서 6~8일 동안 배양한 후 정제수를 5배 첨가하여 40℃에서 24시간 동안 건조하여 표고버섯 발효물을 제조하였다.(2) Shiitake mixed with 40% by weight of shiitake mycelium, 15% by weight of purified water, 5% by weight of lactose, 10% by weight of loquat leaf powder, 20% by weight of rice bran powder and 10% by weight of olive leaf powder based on the total weight of the shiitake mixture After culturing the mixture at 34-40° C. for 6-8 days, purified water was added 5 times and dried at 40° C. for 24 hours to prepare a fermented shiitake mushroom.

(3) 계분 60 중량%와 상기 (1)단계의 분쇄한 난각 발효물 20 중량% 및 상기 (2)단계의 제조한 표고버섯 발효물 20 중량%를 혼합하였다.(3) 60 wt% of chicken manure, 20 wt% of the pulverized egg shell fermented product of step (1), and 20 wt% of the fermented shiitake mushroom prepared in step (2) were mixed.

비교예 1. 계란껍질을 이용한 비료Comparative Example 1. Fertilizer using eggshell

(1) 난각 혼합물 총 중량 기준으로, 분쇄한 계란껍질 50 중량%에 정제수 30 중량% 및 락토스 20 중량%를 혼합한 난각 혼합물에 각각의 미생물의 농도가 105 cfu/㎖씩 포함된 유용 미생물 배양액(Bacillus subtilis, Lactobacillus parafarraginis, Lactobacillus rhamnosus, Lactobacillus plantarum 및 Saccharomyces cerevisiae) 5%(v/w) 접종한 후 40℃에서 45일 동안 교반하면서 발효시킨 난각 발효물을 건조한 후 분쇄하였다.(1) Based on the total weight of the eggshell mixture, an eggshell mixture containing 50% by weight of pulverized eggshell, 30% by weight of purified water and 20% by weight of lactose, each of which contains 10 5 cfu/ml of each microorganism concentration. ( Bacillus subtilis , Lactobacillus parafarraginis, Lactobacillus rhamnosus, Lactobacillus plantarum and Saccharomyces cerevisiae ) After inoculation with 5% (v/w), the egg shell fermented product fermented with stirring at 40 ° C. for 45 days was dried and pulverized.

(2) 표고 혼합물 총 중량 기준으로, 표고버섯 균사체 40 중량%에 정제수 15 중량%, 락토스 5 중량% 및 쌀겨 분말 40 중량%를 혼합한 표고 혼합물을 37℃에서 7일 동안 배양한 후 정제수를 5배 첨가하여 40℃에서 24시간 동안 건조하여 표고버섯 발효물을 제조하였다.(2) Based on the total weight of the shiitake mixture, a shiitake mixture in which 40% by weight of shiitake mushroom mycelium is mixed with 15% by weight of purified water, 5% by weight of lactose, and 40% by weight of rice bran powder was cultured at 37° C. for 7 days, and then purified water was added to 5 The pear was added and dried at 40° C. for 24 hours to prepare a fermented shiitake mushroom.

(3) 계분 60 중량%와 상기 (1)단계의 분쇄한 난각 발효물 20 중량% 및 상기 (2)단계의 제조한 표고버섯 발효물 20 중량%를 혼합하였다.(3) 60 wt% of chicken manure, 20 wt% of the pulverized egg shell fermented product of step (1), and 20 wt% of the fermented shiitake mushroom prepared in step (2) were mixed.

비교예 2. 계란껍질을 이용한 비료Comparative Example 2. Fertilizer using eggshell

(1) 난각 혼합물 총 중량 기준으로, 분쇄한 계란껍질 50 중량%에 정제수 30 중량%, 락토스 12 중량% 및 나한과 분말(오붐주식회사) 8 중량%를 혼합한 난각 혼합물에 각각의 미생물의 농도가 105 cfu/㎖씩 포함된 유용 미생물 배양액(Bacillus subtilis, Lactobacillus parafarraginis, Lactobacillus rhamnosus, Lactobacillus plantarum 및 Saccharomyces cerevisiae) 5%(v/w) 접종한 후 40℃에서 45일 동안 교반하면서 발효시킨 난각 발효물을 건조한 후 분쇄하였다.(1) Based on the total weight of the eggshell mixture, the concentration of each microorganism was found in the eggshell mixture of 50% by weight of crushed eggshell, 30% by weight of purified water, 12% by weight of lactose, and 8% by weight of Nahan and powder (Oboom Co., Ltd.) Fermented egg shells were inoculated with 5% (v/w) of useful microorganism culture ( Bacillus subtilis , Lactobacillus parafarraginis, Lactobacillus rhamnosus, Lactobacillus plantarum, and Saccharomyces cerevisiae ) containing 10 5 cfu/ml and fermented at 40°C for 45 days with stirring. was dried and then pulverized.

(2) 계분 60 중량%와 상기 (1)단계의 분쇄한 난각 발효물 40 중량%를 혼합하였다.(2) 60 wt% of chicken manure and 40 wt% of the pulverized egg shell fermented product of step (1) were mixed.

비교예 3. 비료Comparative Example 3. Fertilizer

(1) 표고 혼합물 총 중량 기준으로, 표고버섯 균사체 40 중량%에 정제수 15 중량%, 락토스 5 중량%, 비파잎 분말 10 중량%, 쌀겨 분말 20 중량% 및 올리브잎 분말 10 중량%를 혼합한 표고 혼합물을 34~40℃에서 6~8일 동안 배양한 후 정제수를 5배 첨가하여 40℃에서 24시간 동안 건조하여 표고버섯 발효물을 제조하였다.(1) Based on the total weight of the shiitake mixture, 40% by weight of shiitake mushroom mycelium, 15% by weight of purified water, 5% by weight of lactose, 10% by weight of loquat leaf powder, 20% by weight of rice bran powder, and 10% by weight of olive leaf powder After culturing the mixture at 34-40° C. for 6-8 days, purified water was added 5 times and dried at 40° C. for 24 hours to prepare a fermented shiitake mushroom.

(2) 계분 60 중량%와 상기 (1)단계의 제조한 표고버섯 발효물 40 중량%를 혼합하였다.(2) 60 wt% of chicken manure and 40 wt% of the fermented shiitake mushroom prepared in step (1) were mixed.

실험예 1. 비료의 성분 분석 결과Experimental Example 1. Result of component analysis of fertilizer

실시예 1과 비교예 1 내지 3의 비료의 성분 분석한 결과, 모든 비료에서 비소, 카드뮴, 수은은 검출되지 않았고, 납, 크롬, 구리, 니켈 및 아연도 기준치 이하로 나타났다. 또한, 유기물은 모두 40%가 넘게 나타났고, 질소는 모두 20% 이하로 나타나, 유기물 25 중량% 이상, 질소는 유기물 대비 50% 이하인 비료공정규격에 적합하였다.As a result of analyzing the components of the fertilizers of Example 1 and Comparative Examples 1 to 3, arsenic, cadmium, and mercury were not detected in any of the fertilizers, and lead, chromium, copper, nickel and zinc were also found to be below the standard values. In addition, all organic matter appeared to be more than 40%, and all nitrogen was less than 20%, which was suitable for the fertilizer process standards of 25 wt% or more of organic matter and 50% or less of nitrogen compared to organic matter.

재배예 1. 파프리카 재배 효과Cultivation Example 1. Effect of growing paprika

파프리카 식물체에 실시예 1과 비교예 1 내지 3의 비료를 각각 7일 간격으로 3번 동일량으로 시비하였다. 마지막 시비 후 일주일 뒤 파프리카를 수확하였다. 수확한 파프리카의 비타민 C 함량을 비교하였고, 항산화 활성은 DPPH 라디칼 소거능에 의해 측정하였다.The paprika plants were fertilized with the fertilizers of Example 1 and Comparative Examples 1 to 3 in the same amount three times at 7-day intervals, respectively. Paprika was harvested a week after the last fertilization. Vitamin C content of harvested paprika was compared, and antioxidant activity was measured by DPPH radical scavenging ability.

파프리카 재배 효과 비교Comparison of Paprika Cultivation Effects 구분division 비타민 C 함량(㎎/100g)Vitamin C content (mg/100g) DPPH 라디칼 소거능(%)DPPH radical scavenging ability (%) 비료 무처리no fertilizer 126.0126.0 28.128.1 실시예 1Example 1 186.9186.9 48.548.5 비교예 1Comparative Example 1 158.2158.2 43.343.3 비교예 2Comparative Example 2 142.3142.3 41.441.4 비교예 3Comparative Example 3 135.1135.1 39.139.1

그 결과, 실시예 1의 비료를 살포하여 재배한 파프리카는 비타민 C 함량이 높고 항산화 활성이 우수하여 기능성이 효과적으로 개선됨을 확인하였다.As a result, it was confirmed that the paprika grown by spraying the fertilizer of Example 1 had a high vitamin C content and excellent antioxidant activity, so that the functionality was effectively improved.

재배예 2. 배추 재배 효과Cultivation Example 2. Effect of Cultivation of Chinese Cabbage

배추 모종을 밭에 이식한 후 실시예 1과 비교예 1 내지 3의 비료를 각각 10일 간격으로 3회 경엽처리하고, 마지막 처리 후 일주일 뒤 배추를 수확하였다. 수확한 배추 잎의 항산화 활성은 DPPH 라디칼 소거능에 의해 측정하였다.After the cabbage seedlings were transplanted into the field, the fertilizers of Example 1 and Comparative Examples 1 to 3 were foliated three times at 10-day intervals, respectively, and Chinese cabbage was harvested a week after the last treatment. Antioxidant activity of harvested cabbage leaves was measured by DPPH radical scavenging activity.

배추 재배 효과 비교Comparison of Cabbage Cultivation Effect 구분division DPPH 라디칼 소거능(%)DPPH radical scavenging ability (%) 비료 무처리no fertilizer 22.322.3 실시예 1Example 1 39.039.0 비교예 1Comparative Example 1 32.232.2 비교예 2Comparative Example 2 30.230.2 비교예 3Comparative Example 3 28.828.8

그 결과, 실시예 1의 비료를 처리하여 재배한 배추가 가장 높은 항산화 활성을 나타내어, 실시예 1의 비료는 고품질의 작물 재배에 유용하게 사용할 수 있음을 확인할 수 있었다.As a result, it was confirmed that the Chinese cabbage grown by treating the fertilizer of Example 1 showed the highest antioxidant activity, and the fertilizer of Example 1 could be usefully used for growing high-quality crops.

Claims (4)

(1) 난각 혼합물 총 중량 기준으로, 계란껍질 45~55 중량%, 물 27~33 중량%, 락토스 10~14 중량% 및 나한과 분말 6~10 중량%를 혼합한 난각 혼합물에 바실러스 서브틸리스(Bacillus subtilis), 락토바실러스 파라파라기니스(Lactobacillus parafarraginis), 락토바실러스 람노서스(Lactobacillus rhamnosus), 락토바실러스 플란타룸(Lactobacillus plantarum) 및 사카로마이세스 세레비지애(Saccharomyces cerevisiae)로 이루어진 미생물 배양액을 접종한 후 35~45℃에서 40~50일 동안 발효시킨 난각 발효물을 건조한 후 분쇄하는 단계;
(2) 표고 혼합물 총 중량 기준으로, 표고버섯 균사체 35~45 중량%, 물 13~17 중량%, 락토스 4~6 중량%, 비파잎 분말 8~12 중량%, 쌀겨 분말 18~22 중량% 및 올리브잎 분말 8~12 중량%를 혼합한 표고 혼합물을 34~40℃에서 6~8일 동안 배양하고 35~45℃에서 20~28시간 동안 건조하여 표고버섯 발효물을 제조하는 단계; 및
(3) 난각 비료 총 중량 기준으로, 계분 60 중량%와 상기 (1)단계의 분쇄한 난각 발효물 20 중량% 및 상기 (2)단계의 제조한 표고버섯 발효물 20 중량%를 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 난각 비료의 제조방법.(1) Based on the total weight of the eggshell mixture, 45 to 55% by weight of eggshell, 27 to 33% by weight of water, 10 to 14% by weight of lactose, and 6 to 10% by weight of powder with Bacillus subtilis ( Bacillus subtilis ), Lactobacillus parafarraginis ), Lactobacillus rhamnosus ), Lactobacillus plantarum ( Lactobacillus plantarum ) and Saccharomyces cerevisiae consisting of Saccharomyces cerevisiae ) Drying and pulverizing fermented egg shells fermented for 40-50 days at 35-45° C. after inoculation;
(2) based on the total weight of the shiitake mixture, 35 to 45% by weight of shiitake mycelium, 13 to 17% by weight of water, 4 to 6% by weight of lactose, 8 to 12% by weight of loquat leaf powder, 18 to 22% by weight of rice bran powder and Preparing a fermented shiitake mushroom by culturing the shiitake mixture mixed with 8-12 wt% of olive leaf powder at 34-40 ° C. for 6-8 days and drying at 35-45 ° C. for 20-28 hours; and
(3) Based on the total weight of the eggshell fertilizer, mixing 60% by weight of chicken manure, 20% by weight of the pulverized eggshell fermented product of step (1), and 20% by weight of the fermented shiitake mushroom prepared in step (2) above; A method for producing egg shell fertilizer, characterized in that it is prepared by including. 삭제delete 삭제delete 삭제delete
KR1020210010370A 2021-01-25 2021-01-25 Method for producing fertilizer using egg shell and various material KR102371464B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020210010370A KR102371464B1 (en) 2021-01-25 2021-01-25 Method for producing fertilizer using egg shell and various material

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020210010370A KR102371464B1 (en) 2021-01-25 2021-01-25 Method for producing fertilizer using egg shell and various material

Publications (1)

Publication Number Publication Date
KR102371464B1 true KR102371464B1 (en) 2022-03-10

Family

ID=80816173

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020210010370A KR102371464B1 (en) 2021-01-25 2021-01-25 Method for producing fertilizer using egg shell and various material

Country Status (1)

Country Link
KR (1) KR102371464B1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102622524B1 (en) 2023-03-14 2024-01-09 주식회사 티센바이오팜 Methods for separating eggshells and eggshell membranes
KR20240124805A (en) 2023-02-09 2024-08-19 (주)파머프렌즈 Eco-friendly fertilizer composition from waste eggs and Microorganisms, and its manufacturing method
KR20240124806A (en) 2023-02-09 2024-08-19 (주)파머프렌즈 Eco-friendly fertilizer composition from hydrolyzed waste and Microorganisms, and its manufacturing method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130120037A (en) * 2012-04-25 2013-11-04 농업회사법인 주식회사 토산산업 Eco-frienddly organic compound fertilizer using organic materials
KR102110237B1 (en) * 2019-10-24 2020-05-13 천경선 Method for producing functional fermented vinegar with increased antioxidant activity
KR20200094528A (en) * 2019-01-30 2020-08-07 재단법인 장흥군버섯산업연구원 Healthful mixed tea development using fermented herbs, fruits and shiitake

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130120037A (en) * 2012-04-25 2013-11-04 농업회사법인 주식회사 토산산업 Eco-frienddly organic compound fertilizer using organic materials
KR20200094528A (en) * 2019-01-30 2020-08-07 재단법인 장흥군버섯산업연구원 Healthful mixed tea development using fermented herbs, fruits and shiitake
KR102110237B1 (en) * 2019-10-24 2020-05-13 천경선 Method for producing functional fermented vinegar with increased antioxidant activity

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20240124805A (en) 2023-02-09 2024-08-19 (주)파머프렌즈 Eco-friendly fertilizer composition from waste eggs and Microorganisms, and its manufacturing method
KR20240124806A (en) 2023-02-09 2024-08-19 (주)파머프렌즈 Eco-friendly fertilizer composition from hydrolyzed waste and Microorganisms, and its manufacturing method
KR102622524B1 (en) 2023-03-14 2024-01-09 주식회사 티센바이오팜 Methods for separating eggshells and eggshell membranes

Similar Documents

Publication Publication Date Title
KR102371464B1 (en) Method for producing fertilizer using egg shell and various material
KR101016859B1 (en) Antagonistic bacteria and its microbial organic fertilizers to prevent the wilting of serial bananas
KR101365728B1 (en) Environment- friendly organic liquid fertilizer and manufacturing method of the same
CN107794240B (en) Bacillus megaterium, polypeptide agricultural microbial agent thereof, and preparation method and application thereof
KR101039176B1 (en) Antagonistic bacteria for preventing and eliminating the bacterial wilt of continuous cropping tobacco and their microbial organic fertilizer
KR101669599B1 (en) Composition for eliminating odor and heavy metal comprising effective microorganism culture broth as effective component
CN101870608B (en) Continuous cropping resisting biological organic and inorganic compound fertilizer and production method thereof
CN109650986B (en) Carbon-based bio-organic fertilizer for improving degraded vegetable field soil and application thereof
CN103274829A (en) Special compound microbial fertilizer for tomatoes and preparation method thereof
MX2014012524A (en) Use of synergistic microorganisms and nutrients to produce signals that facilitate the germination and plant root colonization of mycorrhizal fungi in phosphorus rich environments.
KR101670651B1 (en) Method for producing fermented liquid fertilizer of manure with reduced odor and heavy metal content using effective microorganism
CN111574283A (en) Microbial fertilizer and preparation method thereof
KR20150098592A (en) Functional microbial fermentation using starfish and seaweed and methods for their preparation
KR20220076124A (en) Promotes onion growth and drought tolerance by bacillus altitudinis h5-9 and uses thereof
CN104478546A (en) Method for utilizing vitex negundo var ineica scrap to culture hericium erinaceus
CN110591937B (en) Antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, method and application
CN111689801A (en) Compound humic acid fertilizer and preparation and application thereof
CN103351188B (en) Preparation method of agricultural organic fertilizer by treating pure straws as raw material
KR20110087920A (en) Solid-type seed medium for tricholoma matsutake and method for cultivating tricholoma matsutake using the same
CN104844285A (en) Method for preparing bio-organic fertilizer for improving immunity of cherry tomatoes
CN101792338A (en) Amino-acid mineral biological fungi compound fertilizer and preparation process thereof
CN110791459A (en) Bacillus subtilis for preventing and controlling continuous cropping lily soil-borne blight and application thereof
KR20110077703A (en) Compost comprising cocopeat and peat, and the preparation method thereof
CN111302857B (en) Water-soluble organic biological bacterial fertilizer for drip irrigation tomatoes and preparation method thereof
CN109956837A (en) A kind of how anti-compound fertilizer of tea seed cake and its preparation method and application

Legal Events

Date Code Title Description
E701 Decision to grant or registration of patent right
GRNT Written decision to grant