KR102028225B1 - 생체분자 분리 - Google Patents

생체분자 분리 Download PDF

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Publication number
KR102028225B1
KR102028225B1 KR1020147022894A KR20147022894A KR102028225B1 KR 102028225 B1 KR102028225 B1 KR 102028225B1 KR 1020147022894 A KR1020147022894 A KR 1020147022894A KR 20147022894 A KR20147022894 A KR 20147022894A KR 102028225 B1 KR102028225 B1 KR 102028225B1
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South Korea
Prior art keywords
liquid
conduit
magnetic particles
capture site
sample
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KR1020147022894A
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English (en)
Korean (ko)
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KR20140120916A (ko
Inventor
키어런 쿠란
데이비드 맥과이어
Original Assignee
젠셀 바이오시스템즈 리미티드
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Publication of KR20140120916A publication Critical patent/KR20140120916A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020147022894A 2012-01-25 2013-01-25 생체분자 분리 KR102028225B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261590499P 2012-01-25 2012-01-25
US61/590,499 2012-01-25
PCT/IB2013/000478 WO2013111016A2 (en) 2012-01-25 2013-01-25 Biomolecule isolation

Publications (2)

Publication Number Publication Date
KR20140120916A KR20140120916A (ko) 2014-10-14
KR102028225B1 true KR102028225B1 (ko) 2019-10-02

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KR1020147022894A KR102028225B1 (ko) 2012-01-25 2013-01-25 생체분자 분리

Country Status (10)

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US (2) US9777269B2 (es)
EP (1) EP2807255B1 (es)
JP (1) JP6141878B2 (es)
KR (1) KR102028225B1 (es)
CN (2) CN104334725A (es)
AU (1) AU2013213349B2 (es)
BR (1) BR112014018187A8 (es)
CA (1) CA2861959A1 (es)
ES (1) ES2642862T3 (es)
WO (1) WO2013111016A2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8465707B2 (en) 2010-07-22 2013-06-18 Gencell Biosystems Ltd. Composite liquid cells
EP2807255B1 (en) 2012-01-25 2017-08-02 Gencell Biosystems Limited Biomolecule isolation
AU2013350823B2 (en) 2012-11-27 2017-11-16 Gencell Biosystems Ltd. Handling liquid samples
EP2994233A2 (en) 2013-05-07 2016-03-16 Gencell Biosystems Limited Stabilisation features
KR20160020559A (ko) 2013-06-18 2016-02-23 젠셀 바이오시스템즈 리미티드 생체분자 단리 및 열가공
CN105723203B (zh) 2013-11-25 2019-10-25 基因细胞生物系统有限公司 磁性分离
GB201321433D0 (en) * 2013-12-04 2014-01-15 Isis Innovation Controling fluid micro compartments
EP3105326A4 (en) 2014-02-10 2018-01-10 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
KR20170038059A (ko) * 2014-08-04 2017-04-05 젠셀 바이오시스템즈 리미티드 3상 유체의 처리
US10207271B2 (en) 2014-08-04 2019-02-19 Gencell Biosystems Ltd. Benchtop nucleic acid library preparation device and methods for using the same
CN106574302A (zh) 2014-08-04 2017-04-19 基因细胞生物系统有限公司 基因组分析装置和使用方法
US10287570B2 (en) * 2014-11-21 2019-05-14 Occam Biolabs, Inc. System and method for collecting a sample of nucleic acid
EP3227461A1 (en) * 2014-12-05 2017-10-11 Amyris, Inc. High-throughput sequencing of polynucleotides
PL413551A1 (pl) * 2015-08-18 2017-02-27 Curiosity Diagnostics Spółka Z Ograniczoną Odpowiedzialnością Sposób i system do izolacji kwasów nukleinowych
WO2017033070A1 (en) 2015-08-26 2017-03-02 Gencell Biosystems Ltd. Composite liquid cell (clc) supports, and methods of making and using the same
JP6884562B2 (ja) 2016-11-30 2021-06-09 シスメックス株式会社 検体処理方法および検体処理装置
CN115873691B (zh) * 2023-02-24 2023-05-16 北京凡知医学科技有限公司 一种微流控芯片和核酸提取纯化方法及装置

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110104816A1 (en) * 2008-05-03 2011-05-05 Advanced Liquid Logic, Inc. Method of Loading a Droplet Actuator

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1575805A (en) * 1976-03-12 1980-10-01 Technicon Instr Automatic diagnostic apparatus
WO1989007880A2 (en) * 1988-02-10 1989-09-08 Nygene Corporation Process for producing biochemicals
ES2130177T3 (es) 1991-08-10 1999-07-01 Medical Res Council Tratamiento de poblaciones de celulas.
US5505877A (en) 1991-10-15 1996-04-09 Krivohlavek; Dennis Making multiple phase emulsion or gel
DE69838521T2 (de) 1997-07-07 2008-05-21 Medical Research Council Methode zur Erhöhung der Konzentration von Nucleinsäuremolekülen
US5902227A (en) 1997-07-17 1999-05-11 Intevep, S.A. Multiple emulsion and method for preparing same
US5980936A (en) 1997-08-07 1999-11-09 Alliance Pharmaceutical Corp. Multiple emulsions comprising a hydrophobic continuous phase
EP1053784A3 (en) 1999-05-21 2001-05-23 Bruker Daltonik GmbH Processing samples in solutions having defined small contact area with support
CA2379503A1 (en) * 1999-08-20 2001-03-01 Promega Corporation Simultaneous isolation and quantitation of dna
DE10111520B4 (de) * 2001-03-09 2004-01-15 Chemagen Biopolymer-Technologie Aktiengesellschaft Verfahren zur Aufreinigung von Biomolekülen mit Hilfe magnetischer Partikel
EP1485204B1 (en) 2002-02-22 2006-02-08 Biodot, Inc. Method and apparatus for dispersing reagent droplets below a fluid surface using non-contact dispensing
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
EP2283917B1 (en) 2002-05-09 2021-12-15 The University of Chicago Device for pressure-driven plug transport and reaction
EP2159285B1 (en) 2003-01-29 2012-09-26 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
GB0315438D0 (en) 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
EP1707965A1 (en) * 2004-01-15 2006-10-04 Japan Science and Technology Agency Chemical analysis apparatus and method of chemical analysis
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
JP2006010332A (ja) * 2004-06-22 2006-01-12 Eiichi Tamiya 微小容量の試料溶液を形成する方法
EP2248578B1 (en) 2005-03-04 2012-06-06 President and Fellows of Harvard College Method for forming multiple emulsions
WO2007024778A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device, system and method for depositing processed immiscible-fluid-discrete-volumes
US8613889B2 (en) 2006-04-13 2013-12-24 Advanced Liquid Logic, Inc. Droplet-based washing
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8637317B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Method of washing beads
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
WO2007120241A2 (en) * 2006-04-18 2007-10-25 Advanced Liquid Logic, Inc. Droplet-based biochemistry
US8685754B2 (en) 2006-04-18 2014-04-01 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for immunoassays and washing
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
EP2029781A4 (en) * 2006-05-26 2010-06-30 Althea Technologies Inc BIOCHEMICAL ANALYSIS OF PARTITIONED CELLS
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
CN102014871A (zh) 2007-03-28 2011-04-13 哈佛大学 乳液及其形成技术
US20090023189A1 (en) 2007-05-18 2009-01-22 Applera Corporation Apparatus and methods for preparation of subtantially uniform emulsions containing a particle
US20100227323A1 (en) * 2007-07-30 2010-09-09 Cornell Research Foundation, Inc. Microchannel detection device and use thereof
WO2009061372A1 (en) 2007-11-02 2009-05-14 President And Fellows Of Harvard College Systems and methods for creating multi-phase entities, including particles and/or fluids
US8815576B2 (en) 2007-12-27 2014-08-26 Lawrence Livermore National Security, Llc. Chip-based sequencing nucleic acids
US8067176B2 (en) * 2008-01-25 2011-11-29 Shimadzu Corporation Microchemistry reaction method
US8497065B2 (en) * 2008-02-15 2013-07-30 Life Technologies Corporation Methods and kits for extraction of DNA
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
EP2286228B1 (en) 2008-05-16 2019-04-03 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for manipulating beads
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
WO2010121307A1 (en) 2009-04-21 2010-10-28 The University Of Queensland Complex emulsions
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
JP5573335B2 (ja) 2010-04-28 2014-08-20 株式会社島津製作所 磁性体粒子操作デバイス及び磁性体粒子操作方法
US8465707B2 (en) 2010-07-22 2013-06-18 Gencell Biosystems Ltd. Composite liquid cells
US20130096035A1 (en) 2010-07-26 2013-04-18 The Johns Hopkins University Self-sustained fluidic droplet cassette and system for biochemical assays
US9266104B2 (en) 2011-02-11 2016-02-23 Raindance Technologies, Inc. Thermocycling device for nucleic acid amplification and methods of use
EP2807255B1 (en) 2012-01-25 2017-08-02 Gencell Biosystems Limited Biomolecule isolation
WO2013192351A1 (en) 2012-06-20 2013-12-27 Bio-Rad Laboratories, Inc. Stabilized droplets for calibration and testing
EP2703497B1 (en) 2012-08-31 2016-06-22 Roche Diagniostics GmbH Microfluidic chip, device and system for the generation of aqueous droplets in emulsion oil for nucleic acid amplification
WO2014039587A1 (en) 2012-09-05 2014-03-13 Bio-Rad Laboratories, Inc. Systems and methods for stabilizing droplets
US9840734B2 (en) 2012-10-22 2017-12-12 Raindance Technologies, Inc. Methods for analyzing DNA
AU2013350823B2 (en) 2012-11-27 2017-11-16 Gencell Biosystems Ltd. Handling liquid samples
US9427737B2 (en) 2012-12-14 2016-08-30 Bio-Rad Laboratories, Inc. Methods and compositions for using oils for analysis and detection of molecules
EP2994233A2 (en) 2013-05-07 2016-03-16 Gencell Biosystems Limited Stabilisation features
KR20160020559A (ko) 2013-06-18 2016-02-23 젠셀 바이오시스템즈 리미티드 생체분자 단리 및 열가공
US20160193607A1 (en) 2013-11-25 2016-07-07 Gencell Biosystems Ltd. Transportable composite liquid cells
CN105723203B (zh) 2013-11-25 2019-10-25 基因细胞生物系统有限公司 磁性分离
EP3105326A4 (en) 2014-02-10 2018-01-10 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110104816A1 (en) * 2008-05-03 2011-05-05 Advanced Liquid Logic, Inc. Method of Loading a Droplet Actuator

Also Published As

Publication number Publication date
WO2013111016A3 (en) 2013-10-31
ES2642862T3 (es) 2017-11-20
US20180016573A1 (en) 2018-01-18
US10081808B2 (en) 2018-09-25
BR112014018187A2 (es) 2017-06-20
CN104334725A (zh) 2015-02-04
WO2013111016A2 (en) 2013-08-01
CN110511923A (zh) 2019-11-29
JP2015508154A (ja) 2015-03-16
EP2807255A2 (en) 2014-12-03
US9777269B2 (en) 2017-10-03
AU2013213349A1 (en) 2014-08-14
BR112014018187A8 (pt) 2017-07-11
AU2013213349B2 (en) 2018-02-01
KR20140120916A (ko) 2014-10-14
CA2861959A1 (en) 2013-08-01
JP6141878B2 (ja) 2017-06-07
EP2807255B1 (en) 2017-08-02
US20140371107A1 (en) 2014-12-18

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