KR101867479B1 - Composition for skin external application containing complex of herbal extracts - Google Patents

Abstract

The present invention relates to a composition for external application for skin containing an extract of herbal medicine comprising herbal medicine herb extract as an active ingredient, and more particularly to a composition for external application for skin comprising an extract of herbal medicine comprising herbal medicine extract of herb, An effect of enhancing skin barrier function, and an effect of inducing the differentiation of dermal keratinocyte.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a composition for skin external application containing a complex herbal extract as an active ingredient,

The present invention relates to a composition for external application for skin containing an extract of herbal medicine comprising herbal medicine herb extract as an active ingredient, and more particularly to a composition for external application for skin comprising an extract of herbal medicine comprising herbal medicine extract of herb, An effect of enhancing skin barrier function, and an effect of inducing the differentiation of dermal keratinocyte.

Skin plays a very important role as a barrier function to protect individuals from the outside. The barrier function is a protection function for protecting against various stimuli (chemicals, air pollutants, dry environment, ultraviolet rays, etc.) from the outside and excessive divergence of water in the body through the skin. Such protection function is a function of protecting the stratum corneum The function can be maintained only when it is normally formed.

The outermost stratum corneum (horney layer) of the epidermis is formed from keratinocytes, and consists of keratinocytes with complete differentiation and a surrounding lipid layer (Marcelo CL et al . , J. Invest . Dermatol . 80 , pp 37-44, 1983).

The keratinocyte is a characteristic cell in which basal cells continuously proliferating in the lowest epidermis undergo a stepwise change in morphology and function and are elevated to the surface of the skin. After a certain period of time, And the new keratinocyte replaces its function. This repetitive sequence of changes is called "epidermal cell differentiation" or "keratinization".

In addition, during keratinization, keratinocytes form natural moisturizing factors (NMF) and intercellular lipids (ceramides, cholesterol, fatty acids) and form stratum corneum, which gives firmness and flexibility to the stratum corneum, barrier function.

Such stratum corneum can easily lose its function due to environmental factors such as excessive washing, bathing and other lifestyle factors, dry air pollutants, and endogenous diseases such as atopic skin and senile skin. In fact, Due to the increasing number of factors, dry skin symptoms and their complaints have been increasing recently.

Therefore, in order to maintain proper skin moisture, various studies have been made to supply water from the outside or to prevent water loss from the body. Various moisturizers having moisture retention ability have been developed. As a skin moisturizing agent, it is common to use a substance capable of increasing water retention in the stratum corneum such as ceramide and essential fatty acid and lipid complex (Rawlings AV et al . , J. Invest . Dermatol . , 5, pp731- 741, 1994).

However, recently, the risk factors for skin are increasing, and changes in dietary patterns slow down the generation and decline of the stratum corneum, and the function of the keratinocyte is reduced, so that the amount of the moisturizing factor and lipid in the stratum corneum is decreased, There is an increasing trend in people with skin that can not function as a skin barrier. Such breakdown of the skin barrier function causes various skin diseases such as dry skin, atopic dermatitis, contact dermatitis and psoriasis.

In order to solve the above problems, the present inventors have searched for a substance exhibiting excellent skin moisturizing effect from oriental herb medicine, and found that by controlling the mixing ratio of herb, top leaf, passerine, nuyin and safflower seed, It is possible to further enhance the effects of restoration of damaged skin barrier function and increase in skin moisturizing effect, and thus the present invention has been completed.

Accordingly, it is an object of the present invention to provide a composition for external application for skin, which has effects such as skin moisturizing, skin barrier function enhancement, skin keratinocyte differentiation induction, and the like by mixing and extracting various oriental herbal medicines in an appropriate ratio.

In order to attain the above object, the present invention provides a composition for external application for skin comprising, as an active ingredient, a herbal medicine extract of herbal medicines composed of herb, top leaf, passerine, nuyin and safflower seed.

The herbal medicine herbal extract composition comprising herbal medicine herbal extracts of herb, upper leaf, passerine, noodle, safflower and safflower seeds shows excellent effects in promoting differentiation of keratinocytes, recovery of damaged skin barrier function and increase of skin moisturizing power. Therefore, the composition for external application for skin containing the herbal extract of herbal composition prevents or improves skin dryness, atopic dermatitis, contact dermatitis or psoriasis caused by imperfections of epidermal differentiation, and for skin moisturizing, And may be useful as a cosmetic composition or a pharmaceutical composition for inducing differentiation of keratinocytes.

FIG. 1 is a graph showing the effect of the herbal medicine extract of the present invention on the recovery of damaged skin barrier.
FIG. 2 is a graph showing the effects of the herbal medicine extract of the present invention on the skin moisture content.

The present invention provides a composition for external application for skin comprising, as an active ingredient, a herbal medicine extract of herbal medicines composed of herbaceous, upright, passerine, nasiin and safflower seeds.

Lithospermum erythrorhizon S. et Z., which is used in the present invention, is said to be Chinese herb, grass, persimmon, and purple root. The size is 50 ~ 100cm and the bristles are hanging on the whole. The stem stood straight and the upper branches split into 2 or 3 pieces. Five white sculptures are in the flower throat. The size of the flower is 5 ~ 8mm. Leaves are alternate, thick, with both ends pointed and without saw teeth. The roots are split in the shape of ginseng. It is slightly thin, long fusiform, occasionally branched, 6-10cm long, 5-15mm in diameter. The outer surface is dark purple-dark brown and the layer is rough and thin.

The top leaf used in the present invention is a dried medicinal substance of Morus alba L. or Morus alba L., which is a leaf material of Morus alba L., which disperses wind heat and enriches the lungs. It lowers heat in the liver, It is a medicinal product that has the effect of making your hair grow longer.

The passenger used in the present invention is said to be effective in treating seawater and asthma in a herb while removing mature fruit from Prunus armeniaca belonging to Rosaceae to remove flesh and seed coat and drying only seeds. Especially, it has been reported that it contains a large amount of unsaturated fatty acid and has excellent skin soothing effect and whitening effect.

As used herein, the term " Coix lacryma-jobi " belongs to the genus Picea, and is used for the treatment of diarrhea, abdominal obesity, headache, headache, muscle spasms and the like. The active ingredients include coixol, sitosterol, and the like.

The safflower seed used in the present invention refers to the seed of Carthamus tinctorius and is used as a medicament for the eosinophilia (瘀血) or 경 경 약 약 약 (痛 經 药) which regulates the blood of the gentle nature.

In the present invention, the dandruff extract is prepared by blending the seedlings, the top leaves, the passers-by, the kinsmen, and the safflower seeds at a weight ratio of 1: 2 to 8: 2 to 4: 6 to 8: 0.1 to 1.

The herbal medicine extract of the present invention may be prepared by using one species selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane as the extraction solvent alone or by mixing two or more kinds of solvents And the like.

In addition, the extract used in the present invention may be an extract obtained by further evaporating, spray drying or lyophilizing a liquid substance or a concentrate obtained by heating at a temperature of 50 to 100 ° C for 12 to 154 hours or more under reflux conditions.

The composition for external application for skin of the present invention contains the above-described herb extract in an amount of 0.00001 to 2% by weight based on the total weight of the composition. When the content is less than 0.00001% by weight, the skin moisturizing effect, the skin barrier function enhancing effect and the dermal keratinocyte differentiation inducing effect by the above ingredients can not be obtained. When the content is more than 2% by weight, The increase of the effect is not only small but also there is a problem in the feeling of use of the product and the stability of the formulation.

The composition for external application for skin containing the extract of herbal medicine may be used for moisturizing the skin, for strengthening the skin barrier function and for inducing the differentiation of dermal keratinocyte. Therefore, it is preferable to use the external skin composition for skin dermatosis, atopic dermatitis, Psoriasis, and the like.

The composition for external application for skin according to the present invention can be used for various applications such as softening longevity, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, A cosmetic composition such as a body cream, a body oil, a body essence, a makeup base, a foundation, a hair dye, a shampoo, a rinse or a body cleanser, or a pharmaceutical composition such as an ointment, a gel, a cream, a patch or a spray, But the formulations are not particularly limited.

Each of these formulations may contain various types of bases and additives suitable for formulation of the formulations, such as preservatives, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, And the like, and the kind and amount of these components can be easily selected by the inventor (s). For example, the composition of the present invention can be selected from the group consisting of an antioxidant, an antioxidant, a plant extract, a pH adjuster, alcohol, .

In addition, the composition of the present invention may contain other components such as the above-described herbal medicine extract, which can give a synergistic effect to the main effect, within a range not impairing the intended main effect of the present invention.

In addition, the composition of the present invention may contain a skin absorption promoting substance to increase skin moisturizing effect, skin barrier function enhancing effect, and skin keratinocyte differentiation inducing effect.

The cosmetic of the present invention may further comprise a composition selected from the group consisting of water-soluble vitamins, oiliness vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extracts.

The pharmaceutical composition containing the herbal extract of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the production of pharmaceutical compositions.

The pharmaceutical composition comprising the herbal extract of the present invention may be formulated in any form suitable for pharmaceutical preparations, including ointments, gels, creams, patches or spray preparations, .

The dosage of the formulation varies depending on the age, sex, weight, symptom and administration method of the subject, but it is preferably 1.0 to 3.0 ml per day, and it is preferable to apply it one to five times a day for one month or longer.

On the other hand, the herbal medicine extract of the present invention has little toxicity and side effects and can be safely used for prolonged use for preventive purposes.

Hereinafter, the present invention will be described in more detail by way of Examples and Test Examples, but the present invention is not limited thereto.

[Example 1] Preparation of herbal medicine extract 1

2 kg of the mixture was dried at 50 ° C. for 12 hours in a reflux of 3 times, After extraction, it was immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation. The separated filtrate was concentrated under reduced pressure, and the resulting extract was suspended in water. The filtrate was extracted five times with 1 liter of ether to remove the dye. The aqueous layer was washed with 1-butanol And extracted three times with 500 ml. The whole 1-butanol layer thus obtained was concentrated under reduced pressure to obtain a 1-butanol extract, which was dissolved in a small amount of methanol and then added to a large amount of ethyl acetate, and the resulting precipitate was dried to obtain 120 g of herbal extract.

[Example 2] Production of herbal medicine extract 2

The ratio of dried seedlings, upper leaves, passerines, noodles and safflower seeds was mixed at a weight ratio of 1: 2: 2: 7: 0.1 to prepare 2 kg, and 115 g of herbal extract was obtained by the same method as in Example 1.

[Example 3] Preparation of herbal medicine extracts 3

2 kg was prepared by mixing the ratio of dried seedlings, top leaves, passerine, noodles and safflower seeds at a weight ratio of 1: 4: 3: 7: 1, and extracted in the same manner as in Example 1 to obtain 135 g of herbal extract.

[Comparative Example 1] Preparation of herb extract

2 kg of dried shoots were added, and 5 liters of 80% ethanol aqueous solution was added. The mixture was refluxed three times at 50 캜 for 12 hours, and then immersed at 15 캜 for one day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation. The separated filtrate was concentrated under reduced pressure, and the resulting extract was suspended in water. The filtrate was extracted five times with 1 liter of ether to remove the dye. The aqueous layer was washed with 1-butanol And extracted three times with 500 ml. The whole 1-butanol layer thus obtained was concentrated under reduced pressure to obtain a 1-butanol extract. The extract was dissolved in a small amount of methanol and then added to a large amount of ethyl acetate. The resulting precipitate was dried to obtain 80 g of a weed extract.

[Comparative Example 2] Preparation of an extract of Candida

The extract was dried in the same manner as in Comparative Example 1 to obtain 195 g of a dried extract.

[Comparative Example 3] Preparation of Uinto extract

The extract was extracted from 2 kg of dried noodles in the same manner as in Comparative Example 1 to prepare 210 g of NOWIN extract.

[Comparative Example 4] Preparation of upper leaf extract

The dried top leaves of 2 kg were extracted in the same manner as in Comparative Example 1 to prepare 90 g of leaf extract.

[Comparative Example 5] Preparation of extracts of safflower seeds

The extract was extracted from 2 kg of dried safflower seeds in the same manner as in Comparative Example 1 to prepare 230 g of safflower seed extract.

[Test Example 1] Promotion of keratinocyte differentiation

In order to examine the differentiation promoting effect of the keratinocyte on the extract of herbal medicine according to the present invention, the amount of corned envelope (CE) produced upon differentiation of keratinocytes was measured by absorbance as described below.

First, keratinocytes from a primary culture separated from the epidermis of a newborn baby are placed in a culture flask and adhered to the bottom. After the test material of Table 1 is treated at a concentration of 1 ppm in the culture medium, % ≪ / RTI > until growth. At this time, low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were negative control and positive control, respectively. Then, the cultured cells were harvested, washed with PBS (Phosphate buffered saline), and then suspended in 10 mM Tris-HCl buffer (Tris-HCl, pH 7.4) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) pH 7.4) was added, sonication, boiling, and centrifugation. The precipitate was suspended again in 1 ml of PBS and the absorbance at 340 nm was measured. Separately, a part of the solution after the sonication was taken to measure the protein content and used as a standard in evaluating the degree of cell differentiation. The degree of differentiation of keratinocytes was calculated assuming that the degree of cell differentiation in the negative control group was 100%. The results are shown in Table 1 below.

Test substance The ability to differentiate in keratinocytes (%) Low calcium (0.03 mM) solution
(Negative control) 100 High calcium (1.2 mM) solution
(Positive control group) 210 Example 1 201 Example 2 197 Example 3 205 Comparative Example 1 111 Comparative Example 2 103 Comparative Example 3 95 Comparative Example 4 115 Comparative Example 5 127

As shown in Table 1, when the five herbal extracts of Examples 1 to 3 were treated, the effect of accelerating the differentiation of keratinocytes was higher than that of Comparative Examples 1 to 5 treated with the respective extracts And it is confirmed that the degree is almost similar to the differentiation ability in the positive control group.

[Experimental Example 2] Measurement of skin barrier function and skin moisturizing effect in uninjured mouse skin

In order to measure the effects of the herbal extracts of the present invention on the recovery of damaged skin barrier function due to skin damage and on the increase of skin moisturizing ability, the following experiment was conducted.

First, ketamine acetone was administered to the mice (Charles River, Japan) at 8 to 10 weeks after birth periodically for 5 days twice a day to induce barrier function of the skin, such as experimental animals, and then to an evaporator TEWL (Transepidermal water loss) was measured to determine the amount of transdermal water loss of 40 g / m < ² > / hr or more. Experimental materials were mixed with propylene glycol and ethanol in a ratio of 7: 3 A sample containing 1% of each vehicle (vehicle-treated group) and 1% of each of Examples 1 to 3 and Comparative Examples 1 to 5 was continuously applied for 3 days twice a day at a dose of 200 μl per 5 cm ² of skin area The TEWL was measured every hour, and the results are shown in Fig.

In addition, skin moisture was simultaneously measured using a corneometer (Courage Khazaka, Germany), and the results are shown in FIG. At this time, the skin moisture of the untreated group was also measured.

As shown in FIG. 1, when the above Examples 1 to 3 were treated, the barrier damage was recovered faster than the vehicle and the treatment groups of Comparative Examples 1 to 5 due to the synergistic effect.

As shown in Fig. 2, the amount of skin moisture was also higher in the treatment groups of Examples 1 to 3 than in the vehicle and the treatment groups of Comparative Examples 1 to 5.

Thus, it can be seen that when the dandruff extract is treated, barrier moisture is restored and the amount of skin moisture is increased.

From the above results, it can be confirmed that the herbal medicine extract has excellent effects in promotion of differentiation of keratinocytes, recovery of damaged skin barrier, and increase of skin moisturizing power.

Claims (6)

Herb extract, herb medicine, herbarium, and safflower seed as herb extracts,
Wherein the herbal medicine extract is obtained by mixing the herb extracts, the top leaves, the passerine leaves, the nourishing herbs and the safflower seeds at a weight ratio of 1: 2 to 8: 2 to 4: 6 to 8: 0.1 to 1. delete The cosmetic composition according to claim 1, wherein the herbal extract is contained in an amount of 0.00001 to 2% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the composition is for skin moisturizing. The cosmetic composition according to claim 1, wherein the composition is for enhancing skin barrier function. The cosmetic composition according to claim 1, wherein the composition is for inducing differentiation of dermal keratinocytes.

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