KR101851972B1 - Mixed composition to prevent hair loss and promote growing hair providing natural oriental material including artemisiae apiaceae herba, chrysanthemi zawadskii herba, artemisiae argyi herba, artemisiae capillaris herba, sophorae fructus, cirsii herba, and aucklandiae radix extracts and manufacturing method using the same - Google Patents

Abstract

The composite composition for improving hair loss and preventing alopecia contains a herbal medicine extract containing Cheongho, Seulchiae, Lepidoptera, Injinhwa, Beegeum, Daegeum, and Mokcheong, which is a mixed water containing 1 to 5% of 1,3 butylene glycol in purified water, 5: 5: 5: 1: 1: 0.25, followed by extraction and concentration, in order to obtain a mixture having a mass ratio of 50: 5: 5: 5: 5: 1: 1: 0.25.

Description

TECHNICAL FIELD [0001] The present invention relates to a composite composition for improving hair loss and hair loss prevention, which contains herbal extracts containing a herbal extract, a herbal composition, a herbal composition, APIACEAE HERBA, CHRYSANTHEMI ZAWADSKII HERBA, ARTEMISIAE ARGYI HERBA, ARTEMISIAE CAPILLARIS HERBA, SOPHORAE FRUCTUS, CIRSII HERBA, AND AUCKLANDIAE RADIX EXTRACTS AND MANUFACTURING METHOD USING THE SAME}

More particularly, the present invention relates to a composite composition for preventing hair loss and for increasing the thickness of wool or hair, which comprises the herbal medicine extract as an active ingredient, and a composition for preventing hair loss, And a manufacturing method thereof.

Hair loss is increasing due to hormonal effects, poor blood circulation, malnutrition, drug side effects, scalp damage due to dandruce or bacteria, or genetic factors.

In recent years, irritating chemical cleansing agents and excessive stress have become a major cause of damaging the scalp and hair, and dandruff and scalp itching are also pointed out as major causes of hair loss.

Alopecia can be classified into male pattern alopecia and female pattern hair loss, and alopecia areata or alopecia due to other causes.

As described above, in order to alleviate hair loss, it is attempted to solve the hair loss by promoting blood circulation or using a component that inhibits male hormone. However, a method having a remarkable effect has not been proposed yet, and a kind of side effect There is a problem.

Therefore, there is a need for countermeasures that can provide more fundamental hair loss solution and assure various human stability.

Korean Patent Publication No. 10-2014-0100922 Korean Patent No. 10-0449215

It is an object of the present invention to provide a more effective biostable hair promoting composite composition.

According to an aspect of the present invention for achieving the above object, there is provided a process for producing a water-based emulsion, comprising mixing the emulsion of the present invention, the emulsion, the emulsion, the emulsion, As an extraction solvent, and extracting and concentrating the extract.
The mass ratio of the mixed water of the present invention, the crude oil, the petroleum jelly, the lobster, the phosphorus, the cavity, the supernatant and the wheat is 100: 5 to 30: 5 to 30: 5 to 30: 5 to 30: 5: 1 to 5: 0.5 to 2, followed by extraction and concentration.
It is preferable that the mass ratio of the mixed water of the present invention, the blue lake, the rhinoceros, the lobe, the phosphorus lake, the cone angle, the scale, and the wheat are 50: 5: 5: 5: 5: 1: 1: 0.25.
It is preferable that the mass ratio of the mixed water of the present invention, the blue lake, the rhinoceros, the lobe, the phosphorus lake, the cone angle, the scale, and the wheat are 50: 5: 5: 5: 5: 1: 1: 0.25.

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In the concentration step of the present invention, the total weight of the extract obtained by extracting the mixture is preferably concentrated to about 70% to 80%.

According to the composite composition for preventing and treating alopecia of Alopecia herbaceousum containing the herbal medicine extract, which contains Cheongho, Seulchosha, Lepidoptera, Injinhwa, It is possible to improve the function of the scalp and at the same time to have an effect of inhibiting hair loss and promoting foaming, and there is an advantage that hair loss can be prevented and hair growth can be promoted without stimulating the scalp due to herbal medicine ingredients using herbal medicine materials.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart sequentially showing a method for manufacturing a composite composition for improving hair loss and preventing hair loss, which comprises a herbal medicine extract containing Cheongho, Seonjiao, lobule, Injinheo,

The description of the present invention is merely an example for structural or functional explanation, and the scope of the present invention should not be construed as being limited by the embodiments described in the text. That is, the embodiments are to be construed as being variously embodied and having various forms, so that the scope of the present invention should be understood to include equivalents capable of realizing technical ideas. Also, the purpose or effect of the present invention should not be construed as limiting the scope of the present invention, since it does not mean that a specific embodiment should include all or only such effect.

Meanwhile, the meaning of the terms described in the present application should be understood as follows.

The terms "first "," second ", and the like are intended to distinguish one element from another, and the scope of the right should not be limited by these terms. For example, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component.

It is to be understood that when an element is referred to as being "connected" to another element, it may be directly connected to the other element, but there may be other elements in between. On the other hand, when an element is referred to as being "directly connected" to another element, it should be understood that there are no other elements in between. On the other hand, other expressions that describe the relationship between components, such as "between" and "between" or "neighboring to" and "directly adjacent to" should be interpreted as well.

It is to be understood that the singular " include " or "have" are to be construed as including the stated feature, number, step, operation, It is to be understood that the combination is intended to specify that it does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

In each step, the identification code (e.g., a, b, c, etc.) is used for convenience of explanation, the identification code does not describe the order of each step, Unless otherwise stated, it may occur differently from the stated order. That is, each step may occur in the same order as described, may be performed substantially concurrently, or may be performed in reverse order.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, unless otherwise defined. Commonly used predefined terms should be interpreted to be consistent with the meanings in the context of the related art and can not be interpreted as having ideal or overly formal meaning unless explicitly defined in the present application.

The composite composition for improving hair loss and preventing alopecia, comprising the herbal extract according to the present invention, contains as an active ingredient the following components: Chungho, Rhus japonica, Liliaceae, Inginiae,

Chung Ho is a topical part of Artemisia annua Linne or Artemisia apiacea Hance (Compositae), which is used as an anticancer drug. It is rich in vitamin C, and it is used to increase immunity, Blood pressure lowering action.

Chrysanthemum zawadskii and Chrysanthemum indicum species are included in the genus Chrysanthemum. Autumn is a typical native flower of Autumn. In May, the stem becomes 5 words and on September 9 (lunar month) it becomes 9 words. It is called "九 節 草". It is also called 孫 母 草, because one flower blooms at the end of the stem and the white flower stands out more than the fresh one. It grows wild throughout the country except for the beaches, especially in high and deep mountains. It is a single species as a species, but there are several varieties. It is 10 ~ 60 ㎝ in height and 3 ~ 6 ㎝ in flower diameter. The rocky peridot is a Korean specialty plant which grows in the deep mountain of the middle northern part. It is 20 ㎝ in height and 2 ~ 4 ㎝ in flower diameter. The Rhizoma extract can be obtained by extracting various organs or parts of the Rhizoma root, that is, leaves, flowers, fruits, stems, roots, branches and bark, and preferably extracts of stem and leaves.

The leaf is a plant which dried leaves of Artemisia argyi, A. pronecens var. Orientlis or A. montana and dried young stem of the Asteraceae, It is called the grasshopper, the grasshopper, the grasshopper. The lobe warms the gag blood and the gyeongsam, so the uterus and lower abdomen show weak and bleeding uterine bleeding, pregnancy bleeding, hematemesis, nosebleed, necrotic hemorrhage. Also, it is effective for eczema, skin irritation, eczema, and dermatitis. The pharmacological action is known to have a hemostatic effect, a bacterium action, a bronchial smooth muscle relaxation action, a Jinhae genome action, a sleep action, a uterine excitatory induction action, and an irritable shock protection action. Preferred sites for application in the present invention are leaves and stems.

In Jin-Ho is a medicinal herb that dried above the ground of Artemisia capillaris of Asteraceae. It is acute hepatitis and it is used for symptoms such as fuming, whole body yellow, red urine and few symptoms. It is also used for chronic hepatitis, liver cirrhosis, liver cancer, cholecystitis, and gallbladder stones. It is also used for the symptom of burning of the skin due to skin diseases such as eczema, omnis, ringworm, It has been reported that the pharmacological action is to promote bile secretion, hepatic function regeneration, hepatocyte regeneration, lipolysis, coronary artery dilatation, hypotensive action, antipyretic, diuretic, antimicrobial and experimental carcinoma cells. The portion to which the present invention is applied is a ground portion.

It is a ripe fruit of Sophora japonica Linne (leguminosae), which has the effect of cooling the heat and removing the fire.

Cirsium Cirsium japonicum DC. there is. Ussuriense (Regel) Kitamura or other outpost (Compositae) is a prelude to the plant, the leaf juice is good to eat because it is well known to arthritis, it is also effective to paste the flour into the juice, Boiled water is effective when used as a cleaning agent for stem and hemorrhoids. Thistle roots are finely chopped and dried in the sun, and they are used medicinally. They are used for dryness, tension, inflammation, detoxification, diuretic, etc. They are also effective for neuralgia. They also work as hemostatic agents such as hematemesis and bleeding after drying the leaves.

It is a perennial herbaceous perennial plant belonging to Saussurea lappa Clarke which has the scientific name of Saussurea lappa Clarke. It is a root of root, Of chronic inflammation and intestinal symptoms. It is an important medication to cure stomach cramps and intestinal aphasia. It treats chest and abdomen swollen and sore, vomiting, diarrhea, and withdrawing from the belly to the thigh. It is good to write with a new song when it is indigestion and the upper expansion, the ship is big and it does not vomit even if it is vomit. It has antimicrobial action in the eyes and it is a medicinal substance that is important for the treatment of heterogeneity.

≪ Process for producing composite composition &

The extraction method of the composite composition according to the present invention is exemplified as follows.

(1) a preparation step in which the purified water is cleanly washed with purified water, and then dried at room temperature for a predetermined time,

(2) To 100 kg of mixed solvent (mixed water) containing 1 to 5% by mass of 1,3-butylene glycol in purified water, 5 to 30 kg of Chungho, 5 to 30 kg of Rhizoma root, 5 to 30 kg of lobe, And a mixture solvent of 1 to 5% by mass of 1,3-butylene glycol in purified water and a mixed solvent of a mixture of 1 to 5 kg of beeswax, 1 to 5 kg of beeswax, and 0.5 to 2 kg of wheat bran, The mixture is mixed so that the weight ratio of the main component, the main component, the main component, the main component, the pitch angle, the pitch angle, the grain size and the grain size is from 100: 5 to 30: 5 to 30: 5 to 30: 5 to 30: 1 to 5: 1 to 5: 0.5 to 2, 5: 5: 5: 5: 1: 1: 0.25,

(3) 90 kg of the extract obtained by extracting the mixed mixture at the predetermined temperature and time by the mixing step is extracted by concentrating the whole weight by 70% to 80% by concentrating it to 70 kg again.

The composite composition of the present invention prepared as described above may contain auxiliary components commonly used in the art for imparting additional functions in addition to the above components. For example, it may include, but is not limited to, an anti-dandruff agent, hair root enhancer, hair restorer, scalp care agent, refresher, moisturizer, The auxiliary components may be formulated in a suitable ratio by those skilled in the art to the extent that the object of the present invention is not impaired.

The composite composition of the present invention can be formulated into various shapes known in the art and applied to scalp and eyebrows such as external liquids, creams, ointments, pastes, aerosols, gels, waxes and mascaras And can be formulated into a form that can be applied to scalp and skin such as topical application type and softening longevity which can be applied, nutritional lotion, nutritional cream, massage cream, essence, pack, shampoo, rinse, ampoule and treatment.

The results of the sterilization test and the antifungal activity test of the composite composition according to the present invention will be described below.

< Experimental Example  1 - Sterility Test>

Experimental Example 1 was conducted to test the bactericidal effect of the composite composition according to the present invention. The experiment was conducted in the same manner as Experimental Example 1 except that Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa were used as Brain Heart Broth (DIFCO), and then cultured bacteria were diluted to adjust the initial number of bacteria to 10 ⁵ -10 ⁶ CFU / mL.

More specifically, when the test solution was added to 20 mL of the test solution (complex mixture), and the mixture was allowed to stand at room temperature for 1 hour, the number of bacteria was measured to determine the rate of sterilization to the initial number of bacteria. Then, the number of bacteria was measured using a physiological saline as a control, and the number of bacteria was indicated as the initial number of bacteria.

However, in the initial dilution stage of all experiments, neutralization was carried out using D / E Neutralizing Broth (DIFCO). When bacteria were grown in the medium, the number of bacteria on the medium was multiplied by a dilution bath, . The number of bacteria that did not proliferate was multiplied by the dilution factor in the neutralization stage. The number of bacteria that did not proliferate in the medium was multiplied by the dilution factor in the neutralization stage and expressed as "less than 10 (<10)". The viable cell count was calculated according to the following formula (1), and the bactericidal reduction rate was determined according to (2).

[Equation 1]

(N: number of live cells, C: number of colonies (average number of two colonies), D: number of dilutions (dilution factor of diluted liquid))

&Quot; (2) &quot;

(%) = [(A-B) / A] 100 (R: sterilization reduction rate, A: initial number of bacteria, B: number of bacteria after a certain period of time)

The results of the sterilization test by the above procedure are as shown in Table 1 below.

Results of sterilization test (unit: C.F.U / mL) Sample dilution Test strain Early After 1 hour treatment
(Sterilization power)

Complex mixture Escherichia coli 7.5 × 10 ⁶ <10 (99.9999% or more) Staphylococcus aureus 4.9 × 10 ⁶ <10 (99.9999% or more) Salmonella typhimurium 4.7 × 10 ⁶ <10 (99.9999% or more) Pseudomonas aeruginosa 5.8 × 10 ⁶ <10 (99.9999% or more)

tonic Escherichia coli 7.5 × 10 ⁶ <10 (99.9999% or more) Staphylococcus aureus 4.9 × 10 ⁶ <10 (99.9999% or more) Salmonella typhimurium 4.7 × 10 ⁶ <10 (99.9999% or more) Pseudomonas aeruginosa 5.8 × 10 ⁶ <10 (99.9999% or more)

shampoo Escherichia coli 7.5 × 10 ⁶ <10 (99.9999% or more) Staphylococcus aureus 4.9 × 10 ⁶ <10 (99.9999% or more) Salmonella typhimurium 4.7 × 10 ⁶ <10 (99.9999% or more) Pseudomonas aeruginosa 5.8 × 10 ⁶ <10 (99.9999% or more)

treatment Escherichia coli 7.5 × 10 ⁶ <10 (99.9999% or more) Staphylococcus aureus 4.9 × 10 ⁶ <10 (99.9999% or more) Salmonella typhimurium 4.7 × 10 ⁶ <10 (99.9999% or more) Pseudomonas aeruginosa 5.8 × 10 ⁶ <10 (99.9999% or more)

* (): Sterilization reduction rate (%) = (A-B) / A × 100

Here, A: initial number of bacteria

B: Number of bacteria after a certain time

(1) Sterilization test for Escherichia coli

As a result of the sterilization test for Escherichia coli, the complex mixture was reduced to [<10 (less than 10) CFU / mL] after 1 hour treatment with the initial inoculum [7.5 × 106 CFU / mL].

(2) Sterilization test for Staphylococcus aureus

In the sterilization test for Staphylococcus aureus, the complex mixture was reduced to [<10 (less than 10) CFU / mL] after 1 hour treatment with the initial inoculum [4.9 × 106 CFU / mL].

(3) Sterilization test for Salmonella typhimurium

As a result of the sterilization test for Salmonella typhimurium, the complex mixture was reduced to [<10 (less than 10) CFU / mL] after 1 hour treatment with the initial inoculum [4.7 × 106 CFU / mL].

(4) Sterilization test for Pseudomonas aeruginosa

 The sterilization test for Pseudomonas aeruginosa showed that the complex mixture was reduced to [<10 (less than 10) CFU / mL] after 1 hour treatment with the initial inoculum [5.8 × 106 CFU / mL].

< Experimental Example  2 - Antifungal activity  Halo test>

Experimental Example 2 was used to test the antifungal effect of the complex composition according to the present invention. The pityrosporum ovale (Diclofenac) was cultured in Fluid Thioglycollate Medium (DIFCO) medium.

The pityrosporum ovale bacterium was inoculated uniformly onto the prepared Brewer's Anaerobic Agar (DIFCO) plate medium with sterilized cotton swabs, treated with 20 μl of the stock solution in a sterilized disc (Φ 8.0), discs were placed in the center of the medium, Anaerobic Jar (DIFCO) was used to measure the diameter of the inhibition zones formed after incubation for 48 hours under anaerobic conditions

The result of the above process is read by the following equation (3).

&Quot; (3) &quot;

Growth Low Zone: W =

(Where W is the width of the lower zone in mm, T is the total diameter of the sample and the lower zone in mm, and D is the diameter of the sample)

The result of the antifungal test by the above procedure is as shown in Table 2 below.

Result of antifungal test (Unit: mm) Sample dilution Test strain Total diameter (T) Diameter of sample and disc (D) Width of low ground (W) Complex mixture pityrosporum ovale 16 8 4 tonic pityrosporum ovale 14 8 3 shampoo pityrosporum ovale 14 8 3 treatment pityrosporum ovale 13 8 2.5

※ Low growth zone: W =

W: Width of the low zone (mm)

T: Overall diameter of sample and lowland (mm)

D: Diameter of sample (mm)

(1) Antifungal test results on pityrosporum ovale

As a result of measuring the size of the inhibition zone, the result of measuring the low zone for the pityrosporum ovale and 4mm for the poundrosporum ovale, the sample shampoo was measured 3mm for the pityrosporum ovale, and the sample tonic was low for the pityrosporum ovale As a result of the measurement, 3mm, the sample treatment showed the antifungal activity of 2.5mm against the pityrosporum ovale.

The reagent taphylococcus aureus used in Experimental Example 1 or Experimental Example 2 was purchased from ATTC Co., Ltd., Brain Heart Infusion Broth, Brain Heart Infusion Agar, Trypic Soy Agar, D / E Neutralizing Broth, Fluid Thioglycollate Medium, Brewer's Anaerobic Agar, Gaspak EZ Anaerobe Container System (DIFCO, USA).

< Experimental Example  3 - Follicular  Activity experiment>

Comparative Examples were prepared in the same manner as the complex mixture, tonic, shampoo, and treatment containing the composite composition of the present invention as shown in Table 3 below.

 (Unit: wt%) Sample dilution Composite composition ethanol 1,3-butylene glycol Purified water Example 1 50 (complex mixture) 10 10 Balance Example 2 50 (tonic) 10 10 Balance Example 3 50 (shampoo) 10 10 Balance Example 4 50 (treatment) 10 10 Balance Comparative Example - 10 10 Balance

The cell lines of the hair follicle cells were cultured in a culture vessel under a constant temperature condition after MTT assay was carried out using human dermal papilla cells in a DMEM culture medium.

In the MTT assay, human dermal papilla cells cultured on a dish were cultured for a certain period of time, and 100 ul of each of the cells was added to 96 wells so that the final concentrations of the samples according to Examples and Comparative Examples were as shown in Table 4.

Then, the 96 wells treated with the sample were again cultured for a predetermined period, and MTT solution was added to each well to determine the degree of cell growth. Table 4 shows the cell survival rates of Examples 1 to 4 and Comparative Example, and it can be confirmed that the cell proliferation ability of the milk milk is remarkably high in the example in which the complex mixture according to the present invention is added.

 (unit : %) sample History Type of composite composition Treatment concentration (%) Dairy cattle  cell Proliferative ability ( % ) Example 1 Complex mixture 0.01 117 Example 2 tonic 0.01 115 Example 3 shampoo 0.01 115 Example 4 treatment 0.01 116 Comparative Example No additives 0.01 110

< Experimental Example  4 - Dandruff reduction rate Liver effect test>

To investigate the effect of dandruff, which is known to be directly related to hair loss, liver efficacy tests were conducted on patients with relatively high dandruff. The dandruff cumulative dandruff accumulated for one month was evaluated by comparing the weight of the dandruff with the medicament composed of Examples 1 to 4 and Comparative Example.

&Quot; (4) &quot;

Density of dandruff (%) = [(Weight of dandruff before test (mg) - Weight of dandruff (mg) 1 month after start of test) / Weight of dandruff before test (mg)] * 100

Changes in hair due to use Dandruff reduction rate % ) Hair Thickness variation (mm) Hair density (hairs / cm2) Scalp itching effect Example 1 53.2 0.018 194 ++++ Example 2 48.8 0.012 186 ++++ Example 3 49.3 0.015 188 +++ Example 4 46.9 0.015 192 ++++ Comparative Example 1.3 - 0.002 123 +

(Scalp itching effect: ++++: very significant improvement, +++: significant improvement, ++: somewhat improved, +: no improvement effect)

< Experimental Example  5 - Hair loss prevention effect Liver effect test>

To investigate the effects of hair loss on patients with alopecia, we performed liver tests on hair loss effects for 3 months. Judgment on the effect of preventing hair loss was evaluated and recorded in four stages.

Changes in hair due to use One month old 2 months past Three months past Example 1 ++ +++ ++++ Example 2 + ++ ++++ Example 3 + +++ ++++ Example 4 + ++ +++ Comparative Example + + +

(Hair loss prevention effect: ++++: very remarkable improvement, +++: remarkable improvement, ++: somewhat improved, +: no improvement effect)

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the present invention as defined by the following claims It can be understood that

Claims (7)

Which comprises an extract prepared by mixing extract, concentrate with purified water containing 1,3-butylene glycol in a mass ratio of 1 to 5% as an extraction solvent, mixing the extract, concentrate,
A composition for improving hair loss and preventing hair loss, which contains herbal medicine extracts including Cheongho, Seulhwa, Leaf, Injinhwa, Beech,
The method according to claim 1,
Wherein the mass ratio of the mixed purified water to the crude oil is from 100: 5 to 30: 5 to 30: 5 to 30: 5 to 30: 1: 5: 1 to 5: 0.5 to 2, followed by extraction and concentration
A composition for improving hair loss and preventing hair loss, which contains herbal medicine extracts including Cheongho, Seulhwa, Leaf, Injinhwa, Beech,
3. The method of claim 2,
Wherein the mass ratio of the mixed purified water, the purified water, the rhizome, the lobe, the phosphorus, the cavitation, the supernatant, and the wheat is 50: 5: 5: 5: 5: 1: 1: 0.25
A composition for improving hair loss and preventing hair loss, which contains herbal medicine extracts including Cheongho, Seulhwa, Leaf, Injinhwa, Beech,
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