KR101843554B1 - manufacturing method of amino acid fertilizer using fish skin - Google Patents
manufacturing method of amino acid fertilizer using fish skin Download PDFInfo
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- KR101843554B1 KR101843554B1 KR1020170013922A KR20170013922A KR101843554B1 KR 101843554 B1 KR101843554 B1 KR 101843554B1 KR 1020170013922 A KR1020170013922 A KR 1020170013922A KR 20170013922 A KR20170013922 A KR 20170013922A KR 101843554 B1 KR101843554 B1 KR 101843554B1
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- amino acid
- fish
- skin
- weight
- hydroxide solution
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 35
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 32
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 30
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 57
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 16
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 238000003756 stirring Methods 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000006227 byproduct Substances 0.000 claims abstract description 13
- 238000006243 chemical reaction Methods 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 12
- 238000006386 neutralization reaction Methods 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 10
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims abstract description 8
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 8
- 238000000354 decomposition reaction Methods 0.000 claims abstract description 8
- 229910017604 nitric acid Inorganic materials 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 230000003472 neutralizing effect Effects 0.000 claims abstract description 4
- 238000005406 washing Methods 0.000 claims abstract description 4
- 235000019688 fish Nutrition 0.000 claims description 17
- 241000972773 Aulopiformes Species 0.000 claims description 7
- 235000019515 salmon Nutrition 0.000 claims description 7
- 241001165494 Rhodiola Species 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 9
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 32
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 description 5
- 239000011707 mineral Substances 0.000 description 5
- 235000010755 mineral Nutrition 0.000 description 5
- 150000005846 sugar alcohols Polymers 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 3
- 241000723353 Chrysanthemum Species 0.000 description 3
- 235000007516 Chrysanthemum Nutrition 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 150000001298 alcohols Chemical class 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 235000009604 Chrysanthemum X morifolium Nutrition 0.000 description 2
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 235000013330 chicken meat Nutrition 0.000 description 2
- 239000003245 coal Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000007602 hot air drying Methods 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000003307 slaughter Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 1
- YLRXAIKMLINXQY-ZDUSSCGKSA-O (S)-magnoflorine Chemical compound C1=C(OC)C(O)=C2C3=C(O)C(OC)=CC=C3C[C@@H]3[N+](C)(C)CCC1=C23 YLRXAIKMLINXQY-ZDUSSCGKSA-O 0.000 description 1
- 229940043375 1,5-pentanediol Drugs 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 206010008631 Cholera Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000037740 Coptis chinensis Species 0.000 description 1
- XDVZNDLANFJOQR-UHFFFAOYSA-N Coptisine Natural products O=Cc1c2OCOc2ccc1C=C3/NCCc4cc5OCOc5cc34 XDVZNDLANFJOQR-UHFFFAOYSA-N 0.000 description 1
- 208000002979 Influenza in Birds Diseases 0.000 description 1
- KYEAXNAYHSCLMT-CVVGWEDFSA-N Magnoflorine Natural products C[C@H]1OC=C2[C@@H]3[C@@H]1CN4CCc5c([nH]c6ccccc56)[C@@H]4[C@@H]3OC2=O KYEAXNAYHSCLMT-CVVGWEDFSA-N 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- PTPHDVKWAYIFRX-UHFFFAOYSA-N Palmatine Natural products C1C2=C(OC)C(OC)=CC=C2C=C2N1CCC1=C2C=C(OC)C(OC)=C1 PTPHDVKWAYIFRX-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 description 1
- 229940093265 berberine Drugs 0.000 description 1
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 description 1
- 238000009924 canning Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- XYHOBCMEDLZUMP-UHFFFAOYSA-N coptisine Chemical compound C1=C2C=C(C3=C(C=C4OCOC4=C3)CC3)[N+]3=CC2=C2OCOC2=C1 XYHOBCMEDLZUMP-UHFFFAOYSA-N 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- QUCQEUCGKKTEBI-UHFFFAOYSA-N palmatine Chemical compound COC1=CC=C2C=C(C3=C(C=C(C(=C3)OC)OC)CC3)[N+]3=CC2=C1OC QUCQEUCGKKTEBI-UHFFFAOYSA-N 0.000 description 1
- WCVRQHFDJLLWFE-UHFFFAOYSA-N pentane-1,2-diol Chemical compound CCCC(O)CO WCVRQHFDJLLWFE-UHFFFAOYSA-N 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000000859 sublimation Methods 0.000 description 1
- 230000008022 sublimation Effects 0.000 description 1
- 239000002426 superphosphate Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/002—Fertilisers made from animal corpses, or parts thereof from fish or from fish-wastes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/30—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using irradiation, e.g. solar or nuclear radiation; using electric or magnetic fields
-
- C05G3/0064—
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- High Energy & Nuclear Physics (AREA)
- Physics & Mathematics (AREA)
- Pest Control & Pesticides (AREA)
- Sustainable Development (AREA)
- Toxicology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
Abstract
Description
본 발명은 어피로부터 아미노산 액상비료의 제조방법에 관한 것으로서, 더욱 상세하게는 생선 껍질 등과 같은 어류 부산물을 이용하여 아미노산이 풍부한 아미노산 비료를 제조하는 방법에 관한 것이다. More particularly, the present invention relates to a method for producing amino acid-rich amino acid fertilizers by using fish by-products such as fish hulls and the like.
비료는 식물에 양분을 줄 목적으로 토양에 주는 물질로서, 특히 대량으로 줄 필요가 있는 원소는 질소, 인, 칼륨이며 이들은 비료의 3요소라 불린다. 비료의 3요소를 함유하는 비료를 각각 질소 비료, 인산 비료, 칼리 비료라고 하며, 이들 3요소를 적당한 비율로 함유한 것을 복합 비료라고 하기도 한다.Fertilizers are substances that are given to the soil in order to give nutrients to plants. In particular, the elements that need to be given in large quantities are nitrogen, phosphorus, and potassium, which are called the three elements of fertilizer. Fertilizers containing three elements of fertilizer are called nitrogen fertilizer, phosphoric acid fertilizer, and carly fertilizer, respectively, and those containing these three elements in appropriate proportions are sometimes referred to as compound fertilizers.
화학적 처리에 의해서 공업적으로 생산되는 비료를 화학 비료 또는 인조 비료라고 한다. 주요한 화학 비료로서는 황산암모늄, 석탄 질소, 과인산 석탄, 염화 칼륨, 황산 칼륨 등이 있지만, 최근에는 이것들을 조합한 복합비료로서 제품화된 것이 많다.The fertilizer that is produced industrially by chemical treatment is called chemical fertilizer or artificial fertilizer. Major chemical fertilizers include ammonium sulfate, coal nitrogen, superphosphate coal, potassium chloride, and potassium sulfate, but in recent years, many of them have been commercialized as a compound fertilizer combining these.
식물의 성장을 촉진하기 위한 여러 가지 화학 비료의 사용은 생산량의 증대라는 긍정적인 결과도 가져왔지만, 지속적인 사용과 과다 사용으로 인한 토양의 산성화 및 수질의 부영양화와 같은 부정적인 영향도 유발하게 된다. 또한 식물 병원균의 증식을 억제하거나 사멸시킬 수 있는 기능을 가진 비료는 거의 없기 때문에 독성이 큰 농약의 부가 사용이 필수적이어서 이에 따른 농작물 생산비용의 증가와 생태계의 파괴라는 문제가 대두되고 있다.The use of various chemical fertilizers to promote plant growth has also produced positive results, such as increased production, but also has negative effects such as soil acidification and eutrophication of water due to continued use and overuse. In addition, few fertilizers have the function of inhibiting or killing the growth of plant pathogens. Therefore, the use of toxic pesticides is essential, resulting in an increase in crop production costs and destruction of ecosystems.
따라서, 비교적 생태계의 교란이 적은 식물이나 동물성 물질을 이용한 천연물계의 비료나 살균성을 지닌 기능성의 비료를 제조하는 방법의 개발이 절실하게 요구된다.Therefore, there is a desperate need to develop a method for producing a fertilizer having a relatively low ecosystem disturbance or a fertilizer having a natural material system using an animal material or a functional fertilizer having a bactericidal property.
식물이나 동물성 물질을 이용한 비료 중에는 아미노산계 비료가 있는데, 이것은 종래의 무기질 질소 비료를 대체할 수 있으며, 특히 광합성의 과정 없이 식물이 아미노산을 직접 흡수하기 때문에 식물의 성장을 촉진하는데 매우 효과적인 것으로 알려졌다.Fertilizers using plant or animal material have amino acid fertilizers, which can replace conventional inorganic nitrogen fertilizers and are known to be very effective in promoting plant growth, especially because plants absorb amino acids directly without photosynthesis.
대한민국 공개특허 제10-2015-0012666호에는 도축 폐기물을 이용한 아미노산 비료의 제조방법이 개시되어 있다. Korean Patent Publication No. 10-2015-0012666 discloses a method for producing amino acid fertilizer using slaughter waste.
상기 아미노산 비료의 제조방법은 소, 돼지, 닭 등의 육상가축 유래 아미노산으로 광우병, 돼지콜레라, 조류인플루엔자와 같은 병원성 인자에 대한 안전성 문제의 우려가 있다. The method for producing the amino acid fertilizer is an amino acid derived from a livestock animal such as a cattle, a pig, a chicken, etc., and there is a concern about a safety problem against pathogenic factors such as mad cow disease, pig cholera and avian influenza.
본 발명은 상술한 문제점을 개선하고자 창출된 것으로서, 생선 껍질 등과 같은 어류 부산물을 이용하여 아미노산이 풍부한 아미노산 비료를 제조할 수 있는 제조방법을 제공하는 데 그 목적이 있다. It is an object of the present invention to provide a method for producing amino acid-rich amino acid fertilizer using fish by-products such as fish hulls.
상기의 목적을 달성하기 위한 본 발명의 어피로부터 아미노산 액상비료의 제조방법은 어류의 부산물인 어피를 물로 세척하여 이물질을 제거한 다음 0.1 내지 0.2M 수산화나트륨 용액에 투입하여 6 내지 24시간 동안 교반하여 상기 어피에 부착된 비늘을 탈리시키는 전처리단계와; 상기 어피를 건조시킨 다음 분쇄하여 어피 분말을 수득하는 분쇄단계와; 상기 어피 분말 100중량부에 대하여 수산화칼륨 용액 100 내지 300중량부를 반응조에 투입한 후 상기 반응조 내부에 초음파를 가하면서 1 내지 3일 동안 교반하여 단백질을 1차로 가수분해시키는 1차분해단계와; 상기 1차분해단계 후 상기 반응조에 수산화나트륨 용액을 투입한 후 교반하여 단백질을 2차로 가수분해시키는 2차분해단계와; 상기 2차분해단계에서 수득한 아미노산 용액에 질산을 혼합하여 중화시키는 중화단계;를 포함한다.In order to accomplish the above object, the present invention provides a method for producing amino acid liquid fertilizer, which comprises washing a fish by-product, which is a byproduct of fish, with water, removing the foreign substance, adding the mixture to 0.1 to 0.2M sodium hydroxide solution, A pretreatment step of desorbing scales attached to the skin; A crushing step of drying and crushing the skin to obtain an artificial powder; A primary disaggregation step of initially adding 100 to 300 parts by weight of a potassium hydroxide solution to 100 parts by weight of the skin powder, stirring the mixture for 1 to 3 days while applying ultrasonic waves to the inside of the reaction vessel to hydrolyze the protein first; A secondary disaggregation step in which the sodium hydroxide solution is added to the reaction tank after the primary disaggregation step and then stirred to hydrolyze the protein in a second order; And a neutralization step of neutralizing the amino acid solution obtained in the secondary decomposition step by mixing nitric acid.
상기 어피는 연어 껍질인 것을 특징으로 한다.Characterized in that the skin is a salmon skin.
상기 중화단계 후 상기 아미노산 용액 100중량부에 대하여 황련 추출물 1 내지 10중량부를 첨가하여 혼합하는 첨가단계;를 더 포함한다. And adding and mixing 1 to 10 parts by weight of a Chinese Rhodiola extract to 100 parts by weight of the amino acid solution after the neutralization step.
상술한 바와 같이 본 발명은 생선 껍질 등과 같은 어류 부산물을 이용하여 아미노산이 풍부한 아미노산 비료를 제조할 수 있다. As described above, the present invention can produce amino acid-rich amino acid fertilizers using fish by-products such as fish shells and the like.
또한, 본 발명은 폐기물인 어피 부산물을 유용한 자원으로 활용할 수 있는 장점을 갖는다. In addition, the present invention has an advantage of being able to utilize waste byproduct as a useful resource.
이하, 본 발명의 바람직한 실시 예에 따른 어피로부터 아미노산 액상비료의 제조방법에 대하여 구체적으로 설명한다. Hereinafter, a method for producing an amino acid liquid fertilizer from an artificial soil according to a preferred embodiment of the present invention will be described in detail.
본 발명의 일 예에 따른 어피로부터 아미노산 액상비료의 제조방법은 어류의 부산물인 어피를 물로 세척하여 이물질을 제거한 다음 0.1 내지 0.2M 수산화나트륨 용액에 투입하여 6 내지 24시간 동안 교반하여 상기 어피에 부착된 비늘을 탈리시키는 전처리단계와; 상기 어피를 건조시킨 다음 분쇄하여 어피 분말을 수득하는 분쇄단계와; 상기 어피 분말 100중량부에 대하여 수산화칼륨 용액 100 내지 300중량부를 반응조에 투입한 후 상기 반응조 내부에 초음파를 가하면서 1 내지 3일 동안 교반하여 단백질을 1차로 가수분해시키는 1차분해단계와; 상기 1차분해단계 후 상기 반응조에 수산화나트륨 용액을 투입한 후 교반하여 단백질을 2차로 가수분해시키는 2차분해단계와; 상기 2차분해단계에서 수득한 아미노산 용액에 질산을 혼합하여 중화시키는 중화단계;를 포함한다. 각 단계별로 구체적으로 살펴본다.In the method of manufacturing amino acid liquid fertilizer from the skin according to an embodiment of the present invention, the skin, which is a byproduct of fish, is washed with water to remove the foreign substance, and then it is put into 0.1 to 0.2M sodium hydroxide solution and stirred for 6 to 24 hours to attach A pretreatment step of desorbing the scales; A crushing step of drying and crushing the skin to obtain an artificial powder; A primary disaggregation step of initially adding 100 to 300 parts by weight of a potassium hydroxide solution to 100 parts by weight of the skin powder, stirring the mixture for 1 to 3 days while applying ultrasonic waves to the inside of the reaction vessel to hydrolyze the protein first; A secondary disaggregation step in which the sodium hydroxide solution is added to the reaction tank after the primary disaggregation step and then stirred to hydrolyze the protein in a second order; And a neutralization step of neutralizing the amino acid solution obtained in the secondary decomposition step by mixing nitric acid. Each step will be examined in detail.
1. 전처리단계1. Pre-processing step
본 발명은 아미노산 공급원으로 어류를 이용한다. 어류 유래의 아미노산은 소, 돼지, 닭 등의 육상가축 유래 아미노산에 비해 상대적으로 병원성 인자에 대한 안전성이 높다는 장점을 갖는다. The present invention utilizes fish as an amino acid source. The amino acid derived from fish has an advantage that the safety against pathogenic factors is higher than the amino acid derived from the livestock such as cattle, pigs, and chickens.
바람직하게는 어류의 가공시 발생되는 부산물을 이용한다. 이러한 부산물로서 어류의 껍질인 어피를 들 수 있다. 더욱 바람직하게는 어피로 연어의 껍질을 이용할 수 있다. 연어는 회나 통조림용으로 대량가공되므로 연어 껍질은 다른 어류에 비해 재료의 확보가 용이하다. 또한, 연어 가공공정에서 발생되는 연어껍질은 다른 어류에 비해 상대적으로 계절의 영향이 작고 손질작업이 간편하다는 장점을 갖는다. Preferably, by-products generated during processing of fish are used. As such a by-product, there can be mentioned an ear which is a shell of a fish. More preferably, the shell of the salmon can be used as the skin. Since the salmon is processed in large quantities for the sake or canning, the salmon skin is easier to secure the material than other fish. In addition, the salmon bark produced in the salmon processing process has an advantage that the seasonal effect is relatively small and the maintenance work is relatively easy as compared with other fishes.
어피는 주로 생선 가공공정에서 발생되므로 어피에 묻은 각종 이물질이나 혈분 등을 제거하기 위해 깨끗한 물로 2 내지 3회 정도 씻어낸다. Since the skin is mainly produced in the fish processing process, it is washed 2 or 3 times with clean water to remove various foreign matter and blood in the skin.
물로 세척 후 다음 공정인 건조단계를 수행할 수 있으나, 바람직하게 건조 전 어피에 붙은 비늘을 제거한다. 비늘을 제거하지 않고 후술하는 분쇄공정을 수행할 경우 비늘로 인하여 분쇄기 토출구가 막히는 현상이 발생하여 분쇄작업시 많은 시간이 소요될 뿐만 아니라 막힘 현상에 기인한 분쇄물 압력 증가로 분쇄기 토출부 부품이 파손된 현상이 일어나는 등 여러 가지 문제점이 발생한다. 따라서 어피에 붙은 비늘을 제거할 필요성이 있다. 도구를 이용하여 수작업으로 작업자가 일일이 비늘을 제거하면 공정상 어려움과 많은 시간이 소요된다. After washing with water, the next drying step can be carried out, but the scales attached to the skin are preferably removed before drying. When the grinding process described below is performed without removing the scales, the pulverizer discharge port is clogged due to the scales, so that it takes a lot of time in the pulverizing operation and the parts of the pulverizer discharge part are broken due to the increase of the pulverizing water pressure There are various problems such as the occurrence of the phenomenon. Therefore, it is necessary to remove scales attached to the skin. If the scales are removed manually by the operator using the tool, the process will be difficult and time consuming.
따라서 본 발명은 어피에 부착된 비늘을 간단하게 효과적으로 제거할 수 있도록 수산화나트륨 용액을 이용한다. 예를 들어, 물로 세척하여 이물질을 제거한 어피를 0.1 내지 0.2M 수산화나트륨 용액에 투입하여 6 내지 24시간 동안 교반하여 어피에 부착된 비늘을 탈리시킨다. 수산화나트륨 용액에서 어피를 교반하게 되면 생선종류에 따라 다소 차이가 있으나 비늘이 효과적으로 제거된다. 0.1M 수산화나트륨 용액에서는 80 내지 90%의 비늘이 제거되고, 0.2M 수산화나트륨 용액에서는 90 내지 100%의 비늘이 제거된다. 비늘을 분리한 후 다시 물로 세척한다. Therefore, the present invention uses a sodium hydroxide solution so as to simply and effectively remove scales attached to the skin. For example, the skin, which has been washed with water and the foreign substance is removed, is put into a 0.1 to 0.2M sodium hydroxide solution and agitated for 6 to 24 hours to remove the scales attached to the skin. Agitation in the sodium hydroxide solution will effectively remove scales, although there is some difference depending on the fish species. In the 0.1 M sodium hydroxide solution, 80 to 90% of the scale is removed, and in the 0.2 M sodium hydroxide solution, 90 to 100% of the scale is removed. Remove the scale and rinse again with water.
또한, 교반조에 담긴 수산화나트륨 용액에 어피를 투입하여 교반시 초음파를 가해 비늘 제거 속도를 단축시킬 수 있다. 가령, 교반조의 바닥에 초음파 진동자를 설치하여 교반조 내부로 40 내지 60kHz의 초음파를 가하면서 교반할 수 있다. 수산화나트륨 용액에 의해 비늘의 부착력이 약화된 상태에서 초음파 진동이 어피에 가해져 비늘의 제거가 더욱 용이해진다. In addition, the speed of scales removal can be shortened by applying an ultrasonic wave to the sodium hydroxide solution contained in the stirring tank and stirring it. For example, an ultrasonic vibrator may be installed on the bottom of the stirring tank and stirred while applying ultrasonic waves of 40 to 60 kHz into the stirring tank. Ultrasonic vibration is applied to the skin in a state in which the adhesion force of the scales is weakened by the sodium hydroxide solution so that the scales can be more easily removed.
이와 같이 본 발명은 수산화나트륨 용액을 이용하여 비늘을 매우 용이하게 제거함으로써 어피 분쇄시 분쇄기 토출부의 막힘 현상이 없을 뿐만 아니라 막힘 현상에 의한 분쇄기 부품 파손 현상이 발생하지 않는다. 그리고 비늘을 제거하기 위한 수작업을 생략할 수 있어서 제조시간을 단축하고 비용 및 인력을 절감할 수 있다. As described above, according to the present invention, scales can be very easily removed by using a sodium hydroxide solution, so that clogging of the crusher discharging part is not caused at the crushing of the crusher, and the breakage of the crusher parts due to clogging does not occur. And the manual operation for removing the scales can be omitted, thereby shortening the manufacturing time and reducing the cost and manpower.
2. 분쇄단계2. Grinding step
다음으로, 전처리단계에서 전처리된 어피를 건조 후 분쇄시킨다. Next, the pre-treated skin in the pretreatment step is dried and pulverized.
어피는 수분 함량 2 내지 7중량%로 건조시킬 수 있다. 건조방식으로 동결건조 및 열풍건조 방식 등을 적용할 수 있다. 바람직하게는 콜라겐의 변성을 방지하기 위해 동결건조(freeze drying, FD) 방식을 이용한다. The skin can be dried to a moisture content of 2 to 7% by weight. A freeze-drying method and a hot-air drying method can be applied. Preferably, a freeze drying (FD) method is used to prevent denaturation of the collagen.
동결건조 방법으로 -50 내지 -40℃의 온도에서 10 내지 20시간 어피를 급속동결시킨 다음, 0.1 내지 0.5torr의 진공도를 가진 동결건조기에서 약 -40℃에서 48시간 동안 건조시킨다. 이외에도 식품을 제조하는 데 있어서 적용되는 통상적인 동결건조법을 적용할 수 있음은 물론이다. Rapidly frozen at a temperature of -50 to -40 DEG C for 10 to 20 hours, and then dried at about -40 DEG C for 48 hours in a freeze dryer having a degree of vacuum of 0.1 to 0.5 torr. It is needless to say that the conventional freeze-drying method applicable to the manufacture of foods can be applied.
어피를 동결건조시키는 경우 동결된 상태에서 승화에 의하여 수분이 제거되기 때문에 건조된 제품은 가벼운 다공성 구조를 가지며 모양과 크기도 원래의 상태를 유지하고, 열을 가하지 않고 저온에서 처리되기 때문에 고온의 열풍건조에서 일어나는 가용성 성분의 이동, 비효소적 갈변, 단백질의 변성 등이 거의 일어나지 않는다. If the lid is freeze-dried, since the moisture is removed by sublimation in the frozen state, the dried product has a light porous structure and maintains its original shape and size, and is treated at a low temperature without applying heat, Migration of soluble components in drying, nonenzymatic browning, protein denaturation, etc. hardly occurs.
열풍건조의 경우 40 내지 50℃의 열풍으로 어피를 건조시킬 수 있다. In the case of hot air drying, the skin can be dried by hot air at 40 to 50 ° C.
건조된 어피는 분쇄기를 이용하여 적당한 크기로 분쇄하여 어피 분말을 수득한다. 가령 50 내지 150메쉬 입도 크기로 분쇄할 수 있다. The dried skin is pulverized to an appropriate size using a pulverizer to obtain an artificial powder. For example, 50 to 150 mesh size.
3. 1차분해단계 3. 1st disintegration step
어피 분말에 알칼리 용액을 가해 단백질을 1차로 가수분해하여 아미노산을 생성시킨다. An alkaline solution is added to the egg powder to hydrolyze the protein first to produce an amino acid.
알칼리 용액으로 농도 10 내지 20%(w/w)의 수산화칼륨 용액을 이용할 수 있다. A potassium hydroxide solution having a concentration of 10 to 20% (w / w) can be used as the alkali solution.
1차 가수분해를 위해 반응조에 어피분말과 수산화칼륨 용액을 투입한다. 가령, 어피 분말 100중량부에 대하여 수산화칼륨 용액 100 내지 300중량부를 반응조에 투입한 후 1 내지 3일 동안 교반하여 단백질을 분해시킨다. 이때 단백질 분해율을 높이기 위해 반응조 내부로 초음파를 가하면서 교반시키는 것이 바람직하다. 적용가능한 초음파의 처리 조건은 특별히 한정되지는 아니하나, 바람직하게는 40 내지 50W, 40 내지 60kHz의 초음파를 가해준다. For the primary hydrolysis, the reaction mixture is added with the potash and potassium hydroxide solution. For example, 100 to 300 parts by weight of a potassium hydroxide solution is added to 100 parts by weight of an egg powder, and the mixture is stirred for 1 to 3 days to decompose the protein. At this time, in order to increase the protein decomposition rate, it is preferable to stir while adding ultrasonic wave to the inside of the reaction tank. The processing conditions of the applicable ultrasonic waves are not particularly limited, but preferably 40 to 50 W and 40 to 60 kHz are applied.
상술한 알칼리 용액에 의해 어피 내의 단백질은 펩티드나 아미노산으로 1차 분해된다. With the above-described alkali solution, proteins in the skin are firstly digested with peptides or amino acids.
4. 2차분해단계 4. Second disintegration step
1차분해단계 후 반응조에 수산화나트륨 용액을 더 투입한 후 교반하여 단백질을 2차로 가수분해시킨다. After the first disaggregation step, the sodium hydroxide solution is added to the reaction vessel, and the mixture is stirred to hydrolyze the protein secondarily.
1차분해단계를 통해 수득한 1차 아미노산 용액은 점도가 높고 미분해 단백질이 일부 존재하므로 2차분해단계를 통해 용액의 점도를 낮추고 미분해 단백질을 추가적으로 분해시킬 수 있다. Since the primary amino acid solution obtained through the primary disaggregation step has a high viscosity and a part of the undegraded protein, it is possible to lower the viscosity of the solution and further decompose the undegraded protein through the secondary disaggregation step.
1차 아미노산 용액 100중량부에 대하여 0.1M의 수산화나트륨 용액을 10 내지 20중량부를 첨가한다. 수산화나트륨 용액을 첨가한 후 10 내지 20시간 동안 교반할 수 있다. 10 to 20 parts by weight of a 0.1 M sodium hydroxide solution are added to 100 parts by weight of the primary amino acid solution. Sodium hydroxide solution may be added, followed by stirring for 10 to 20 hours.
5. 중화단계 5. Neutralization step
다음으로, 2차분해단계를 통해 수득한 2차 아미노산 용액에 질산을 혼합하여 pH6~8정도로 중화시킨다. Next, nitric acid is neutralized to a pH of about 6 to 8 by mixing the secondary amino acid solution obtained through the secondary disaggregation step with nitric acid.
질산 혼합으로 인해 2차 아미노산 용액 중의 질소(N)의 함량을 보충함과 동시에 2차 아미노산 용액을 중화시킬 수 있다. 질산은 2차 아미노산 용액 100중량부에 대하여 10 내지 40중량부를 첨가할 수 있다. The nitric acid mixture can supplement the content of nitrogen (N) in the secondary amino acid solution and at the same time neutralize the secondary amino acid solution. 10 to 40 parts by weight of nitric acid may be added to 100 parts by weight of the secondary amino acid solution.
그리고 중화과정에서 인산과 미네랄을 더 혼합할 수 있다. 인산은 2차 아미노산 용액 중의 인(P)의 함량을 보충하기 위함이다. 인산은 2차 아미노산 용액 100중량부에 대하여 2 내지 10중량부를 첨가할 수 있다. In addition, phosphoric acid and minerals can be further mixed in the neutralization process. Phosphoric acid is to supplement the content of phosphorus (P) in the secondary amino acid solution. Phosphoric acid may be added in an amount of 2 to 10 parts by weight based on 100 parts by weight of the secondary amino acid solution.
그리고 미네랄은 2차 아미노산 용액 중의 무기물의 함량을 보충하기 위함이다. 첨가되는 미네랄로 붕소, 구리, 철, 망간, 몰리브덴, 아연 중 적어도 어느 하나일 수 있다. 미네랄은 2차 아미노산 용액 100중량부에 대하여 0.01 내지 2중량부를 첨가할 수 있다. And the minerals are to supplement the content of minerals in the secondary amino acid solution. The added minerals may be at least one of boron, copper, iron, manganese, molybdenum, and zinc. The minerals may be added in an amount of 0.01 to 2 parts by weight based on 100 parts by weight of the secondary amino acid solution.
이와 같이 중화단계를 거쳐 아미노산 액상비료를 수득할 수 있다. Thus, the amino acid liquid fertilizer can be obtained through the neutralization step.
한편, 본 발명은 다른 예로 중화단계 후 2차 아미노산 용액 100중량부에 대하여 황련 추출물 1 내지 10중량부를 첨가하여 혼합하는 첨가단계를 더 수행할 수 있다. In another embodiment of the present invention, after the neutralization step, 1 to 10 parts by weight of the extract of Huangyan extract may be added to and mixed with 100 parts by weight of the secondary amino acid solution.
황련(Coptis chinensis)은 여러해살이 초본식물로서 한국, 일본, 중국 등에서 재배하고 있으며, 주성분으로서는 베르베린(berberine), 팔마틴(palmatine), 콥티신(coptisine), 마그노플로린(magnoflorine), 에피베린(epiberine) 등을 함유한다. Coptis chinensis is a perennial herbaceous plant cultivated in Korea, Japan and China. Its main ingredients are berberine, palmatine, coptisine, magnoflorine, epiberine, ) And the like.
황련 추출물은 황련에 추출용매를 가하여 얻을 수 있다. 추출용매로 물, 탄소수 1 내지 4의 저급 알코올, 다가 알코올 또는 이들의 혼합물로부터 선택된 적어도 어느 하나를 이용할 수 있다. 탄소수 1 내지 4의 저급 알코올로 메탄올, 에탄올 등을 이용할 수 있고, 다가 알코올로 부틸렌글리콜 및 프로필렌글리콜, 펜틸렌글리콜 등을 이용할 수 있다. 그리고 혼합물로는 물 및 저급 알코올의 혼합물, 물 및 다가 알코올의 혼합물, 저급 알코올 및 다가 알코올의 혼합물, 또는 물 및 저급알코올 및 다가 알코올의 혼합물을 이용할 수 있다. Chrysanthemum extract can be obtained by adding an extraction solvent to chrysanthemum. As the extraction solvent, at least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture.
일 예로 황련을 잘게 세절한 후 4 내지 10배 중량비의 메탄올에 침지시켜 상온(20~25℃)에서 12 내지 36시간 동안 추출하여 여과하여 황련 추출물을 얻을 수 있다. For example, after finely chopping the chrysanthemum, it is immersed in methanol at a weight ratio of 4 to 10 times, extracted at room temperature (20 to 25 ° C) for 12 to 36 hours, and filtered to obtain a Chrysanthemum morifolium extract.
황련 추출물은 항균 및 항산화 활성이 우수하여 아미노산 액상비료의 기능성을 높일 수 있다. Chrysanthemum morifolium extract has excellent antimicrobial and antioxidant activity and can enhance the functionality of amino acid liquid fertilizer.
이상, 본 발명은 일 실시 예를 참고로 설명되었으나 이는 예시적인 것에 불과하며, 당해 기술분야에서 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 실시 예가 가능하다는 점을 이해할 것이다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation.
Claims (3)
상기 어피를 건조시킨 다음 분쇄하여 어피 분말을 수득하는 분쇄단계와;
상기 어피 분말 100중량부에 대하여 수산화칼륨 용액 100 내지 300중량부를 반응조에 투입한 후 상기 반응조 내부에 초음파를 가하면서 1 내지 3일 동안 교반하여 단백질을 1차로 가수분해시키는 1차분해단계와;
상기 1차분해단계 후 상기 반응조에 수산화나트륨 용액을 투입한 후 교반하여 단백질을 2차로 가수분해시키는 2차분해단계와;
상기 2차분해단계에서 수득한 아미노산 용액에 질산을 혼합하여 중화시키는 중화단계;를 포함하고,
상기 전처리단계는 상기 교반조에 40 내지 60kHz의 초음파를 가하면서 교반하는 것을 특징으로 하는 어피로부터 아미노산 액상비료의 제조방법. A pretreatment step of washing off the by-product, which is a by-product of the fish, with water to remove impurities, adding the solution to a 0.1 to 0.2M sodium hydroxide solution contained in a stirring tank, and stirring for 6 to 24 hours to remove scale attached to the skin;
A crushing step of drying the crates and crushing them to obtain an artificial crust;
A primary disaggregation step of initially adding 100 to 300 parts by weight of a potassium hydroxide solution to 100 parts by weight of the skin powder, stirring the mixture for 1 to 3 days while applying ultrasonic waves to the inside of the reaction vessel to hydrolyze the protein first;
A secondary disaggregation step in which the sodium hydroxide solution is added to the reaction tank after the primary disaggregation step and then stirred to hydrolyze the protein in a second order;
And a neutralization step of neutralizing the amino acid solution obtained in the secondary decomposition step by mixing nitric acid,
Wherein the pretreatment step is carried out while stirring ultrasonic waves of 40 to 60 kHz into the stirring tank.
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CN110521321A (en) * | 2019-08-30 | 2019-12-03 | 水利部牧区水利科学研究所 | It is a kind of for the surface soil alternative mixtures of open-pit mine blast and its application |
KR102054082B1 (en) * | 2018-12-18 | 2019-12-09 | 김선영 | Liquid fertilizer and manufacturing method thereof |
CN111375618A (en) * | 2018-12-27 | 2020-07-07 | 江苏北斗星环保股份有限公司 | Harmless amino acid-containing aqueous solution for livestock and poultry died of diseases and preparation method thereof |
CN113058977A (en) * | 2021-03-26 | 2021-07-02 | 湖南祥柏生态环保科技有限公司 | Continuous amino acid powder production system |
WO2021258175A1 (en) * | 2020-06-23 | 2021-12-30 | GONÇALVES, Clênio Antônio | Method for producing a fertilizer composition from byproducts from the fishing industry and waste from the processing of fowl, obtained product, and use |
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KR101341704B1 (en) * | 2013-05-02 | 2013-12-16 | 황재호 | Manufacturing process of collagen by using by-products |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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KR102054082B1 (en) * | 2018-12-18 | 2019-12-09 | 김선영 | Liquid fertilizer and manufacturing method thereof |
CN111375618A (en) * | 2018-12-27 | 2020-07-07 | 江苏北斗星环保股份有限公司 | Harmless amino acid-containing aqueous solution for livestock and poultry died of diseases and preparation method thereof |
CN110521321A (en) * | 2019-08-30 | 2019-12-03 | 水利部牧区水利科学研究所 | It is a kind of for the surface soil alternative mixtures of open-pit mine blast and its application |
WO2021258175A1 (en) * | 2020-06-23 | 2021-12-30 | GONÇALVES, Clênio Antônio | Method for producing a fertilizer composition from byproducts from the fishing industry and waste from the processing of fowl, obtained product, and use |
CN113058977A (en) * | 2021-03-26 | 2021-07-02 | 湖南祥柏生态环保科技有限公司 | Continuous amino acid powder production system |
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