KR101247450B1 - Process for preparing of liquid fertilizer using animal blood and liquid fertilizer prepared therefrom - Google Patents

Abstract

PURPOSE: A manufacturing method of liquid fertilizer using blood of domestic animals is provided to reduce manufacturing costs, to enhance the amino acid content of liquid fertilizer, and to maintain fertilizer effect for a long time by using the mixture of mulberry and sugar. CONSTITUTION: A manufacturing method of liquid fertilizer using blood of domestic animals comprises the following steps. Shatter blood of domestic animal by a blood pulverizing device. Add mixture of mulberry and sugar to the pulverized blood. React the blood which is added with mulberry and sugar in the reactor of 52-58°C at a sealing condition for three to five hours. Confirm the pH of reacted blood is 7.0 and cool the reacted blood at the room temperature for three hours. In the adding step, mulberry and sugar are added at a ratio of 3-6 parts by weight per pulverized blood 100.0. In the adding step, the mixing ration of mulberry and sugar is 1:1. [Reference numerals] (AA) Collecting the blood of domestic animals; (BB) Pulverizing blood vessels and others using a blood pulverizing device; (CC) Adding mulberry and sugar; (DD) Protein decomposing reaction in a reactor; (EE) Cooling the reacted blood

Description

   Process for preparing of liquid fertilizer using animal blood and liquid fertilizer prepared therefrom

The present invention relates to a method for producing a liquid fertilizer using livestock blood and a liquid fertilizer prepared therefrom, and more particularly, by reducing the production cost by adding a mixture of Audi and low-cost sugar to the livestock blood generated during the slaughter process, etc. It is rich in amino acid content but does not acidify soil, and it is excellent in growth and growth of plants and does not cause damage due to salt component in soil. It relates to a liquid fertilizer.

Blood from cattle slaughter, such as cattle and pigs, is used as food, but in most cases, it is discarded, and in the process of disposal, groundwater and rivers are contaminated by soil.

       Conventionally, when manufacturing fertilizers using livestock blood, the livestock blood generated in the slaughterhouse is collected, dried, powdered and supplied as a fertilizer in powder form.

As a related art in this regard, Korean Patent Laid-Open Publication No. 2002-0022416 discloses powdered blood powder through fermentation, drying, and grinding of livestock blood collected at a slaughterhouse, and bioceramic, sawdust, rice bran, fermentation plant in powdered blood powder. And a method for producing an organic fertilizer for mixing and fermenting photosynthetic microorganisms, and Japanese Patent Laid-Open No. 2002-0089760 discloses a powder which collects blood of an animal generated in a slaughterhouse and thermally decomposes with sterilization to maintain a water content of 15% or less. A method for producing an amino acid liquid fertilizer, which extracts a blood powder in a state, decomposes the blood powder, dissolves the main component of the fertilizer, and then mixes the zeolite powder, which is a clay mineral, is disclosed. In order to prepare a not only to go through a separate process of drying and powdering, but also to a high salt concentration of blood powder itself It is the fertilizer to be produced by using this, the salts, the residue is excessive, if the try it as fertilizer is soil contaminated with salts, there is a problem that can not be a series.

       In addition, Korean Laid-Open Patent Publication No. 10-2004-0065201 discloses the preparation of amino acid liquid fertilizers by adding chymotrypsin and nitric acid or sulfuric acid, which are proteolytic enzymes, to animal blood or carcasses, and treating them under conditions of high temperature and high pressure and neutralizing them with calcium oxide. Although the method is disclosed, hydrolysis of the protein by strong acid under severe conditions of high temperature and high pressure, most of the cyclic amino acids are destroyed during the decomposition process and the amino acids exist only in the form of free amino acids, resulting in low amino acid content in the final product. Not only is it uneven and its fermentation is not continuous, but the final product of liquid fertilizer shows strong acidity, so it must be neutralized. In the process of neutralization, salt is accumulated in liquid fertilizer. There was a problem that damage occurred.

      Patent No. 10-0980595 includes a first step of collecting and grinding the blood of livestock generated in the slaughterhouse; And a second step of adding the protease extracted from the soybean to the blood pulverized in the first step, putting the protease-added blood into a reactor and reacting in a closed condition and then cooling the blood. A method for producing an amino acid liquid fertilizer using is disclosed, but since the amino acid content is not so high as 19.2%, the ineffectiveness is not sustained, and thus the effect of the active ingredient is not maximized into the plant and the growth efficiency is excellent. It does not represent, and because salt is accumulated in the soil during fertilization, there was a problem that the damage of the series still occurs.

       Korean Patent No. 10-119573 discloses a step of collecting blood from a livestock such as a cow or a pig and then pulverizing blood containing blood vessels or tendons with a blood grinder; Adding 2 to 5 parts by weight of actinidine extracted from the kiwi with 100 parts by weight of enzyme to the blood pulverized in the grinding step, the actinidine is added to maintain blood at 53 ~ 60 ℃ After the reaction in a closed condition for 3-6 hours in a closed condition and the pH of the blood is 7.24, the reaction is terminated, followed by cooling the liquid at room temperature for 3 hours to prepare a liquid fertilizer using livestock blood The method is disclosed, but since it uses expensive actinidine extracted from kiwi as a protease, the production cost is high, the salt concentration in the liquid fertilizer is high, and the acidity (pH) of the liquid fertilizer is 7.24. There is a problem higher than the pH of the soil 5.0 ~ 6.5.

Accordingly, the inventors have repeatedly conducted research and, unlike the prior art, by using a mulberry fruit mulberry and inexpensive sugar can reduce the manufacturing cost of the liquid fertilizer, minimize the breakdown of amino acids contained in the liquid fertilizer, during actual use In addition to preventing acidification of the soil by water-soluble sulfur (S) and not causing damage due to salt accumulation, the present invention was found to have an excellent effect on the growth of plants.

The present invention is to provide a method for producing a liquid fertilizer using the blood of the livestock, which can reduce the production cost by using a mixture of audi and low-cost sugar, high amino acid content while maintaining the effect for a long time and a liquid fertilizer prepared therefrom .

In addition, the present invention is low in water-soluble sulfur (S) and salt content, acidity (pH) is neutral, so it can be applied to the soil for many years, the production of liquid fertilizer using the blood of the livestock showing the excellent growth effect of high mineral content To provide a way.

 Collecting blood from livestock such as cattle or pigs and then crushing them with a blood mill;

Mixing and adding Audi and sugar to the blood ground in the milling step;

Adding the mixed blood of Audi and sugar in the addition step in a reactor maintained at 52 ~ 58 ℃ react for 3 to 5 hours in a sealed condition;

After confirming that the pH of the blood reacted in the reaction step 7.0 provides a method for producing a liquid fertilizer using the blood of the animal, comprising the step of cooling at room temperature for 3 hours.

 In another aspect, the present invention provides a method for producing a liquid fertilizer using livestock blood, characterized in that the addition of sugar and sugar in a ratio of 3 to 6 parts by weight per 100 parts by weight of blood milled in the addition step.

 In another aspect, the present invention provides a method for producing a liquid fertilizer using the blood of the livestock, characterized in that the mixing ratio of the sugar and the sugar in the addition step 1: 1.

Finally, the present invention provides a liquid fertilizer using the blood of the livestock produced by the manufacturing method.

Method for producing a liquid fertilizer using the blood of the livestock according to the invention and the liquid fertilizer prepared therefrom has the following effects of the invention.

First, because it uses a mixture of low-cost audi and sugar, the manufacturing cost is low, the amino acid content is high, and the effect is maintained for a long time shows an excellent effect.

Second, since the acidity (pH) is neutral and low in water-soluble sulfur (S) and salt content, it is possible to suppress acidification of soil by sulfur and grinding damage by salt, so it can be applied to soil for many years. Speed is excellent.

      1 is a flow chart showing a method for producing a liquid fertilizer using the blood of livestock according to an embodiment of the present invention.

DETAILED DESCRIPTION Hereinafter, specific contents of the present invention will be described in detail including a preferred embodiment and a comparative example, which will be described in detail so that a person skilled in the art can easily carry out the invention. It does not mean that the technical spirit and scope of the present invention are limited by this.

Liquid fertilizer manufacturing method using the blood of the livestock according to the present invention is a step of pulverizing the blood of the livestock, in the pulverizing step, the blood mixed with Audi and sugar is added to the reactor maintained at 52 ~ 58 ℃ 3 under closed conditions Reacting for 5 hours;

After confirming that the pH of the blood reacted in the reaction step 7.0 provides a method for producing a liquid fertilizer using the blood of the animal, comprising the step of cooling at room temperature for 3 hours.

     First, the blood grinding step of pulverizing livestock blood is a process of collecting blood from livestock such as pigs and cows generated in a slaughterhouse and then crushing it. The blood, one of by-products generated during slaughter, is collected and decayed. It is stored in a low temperature warehouse kept at 0 ° C for prevention and then used as the main raw material for the manufacture of liquid fertilizers. At this time, since the blood of the livestock is a material that can easily rot even if stored at low temperature, it is preferable to keep the storage period not to exceed two days even in summer.

     On the other hand, the blood of the livestock collected at the slaughterhouse is incidentally mixed with blood vessels and tendons, so the blood itself is easily solidified, so it must be smoothly decomposed through the grinding process before being put into the reactor. If you do not do it, you can put it into the reactor and decompose it, and some coagulated blood, blood vessels and tendons mixed in the blood will not be decomposed by enzymatic action. In the case of applying to plants or crops, a gas may be generated that adversely affects the growth of plants and the like, thereby killing plants and the like.

     When pulverizing blood, a known grinder or the like may be used, but preferably, a grindstone-type blood grinder comprising a pair of solid mineral grinding plates such as diamond is provided on the upper side and the lower side (or left and right). At this time, blood vessels, tendons, etc., which are incidentally mixed with the blood of the livestock, can be completely crushed.

     Subsequently, the blood and the blood vessels, tendons, etc., which are partially coagulated through the grinding process in the blood grinding step are mixed and added with the Audi and sugar. The mulberry fruit is mulberry fruit and is called as "Sangsil" in oriental medicine. It is an obtuse fruit, and it is an oblong form composed of many small aquifolies. It is 1 ~ 2cm long, 0.5 ~ 0.8cm in diameter, yellowish brown, brownish red. Or dark purple with small stems. The small achene is oval, slightly flattened, about 2mm long, about 1mm wide, and is surrounded by four flesh pieces. Audi collects and harvests ripe crops harvested from late spring to early summer and washes them well with water. Sugar is commonly used in the market.

     The amount of audi and sugar added to the ground blood is preferably 3 to 6 parts by weight in 100 parts by weight of blood. If the amount of the audio extract is added to the blood is less than 3 parts by weight, the concentration of enzyme is lower than the protein concentration in the blood. The reaction rate is not fast, and the reaction rate may be slightly improved when it is more than 6 parts by weight, but the reaction rate is no longer accelerated and is relatively disadvantageous in terms of manufacturing cost, which is not preferable. In addition, the mixing ratio of Audi and sugar is preferably 1: 1.

     On the other hand, when using a strong acid such as sulfuric acid, hydrochloric acid, nitric acid as in the conventional method, and forcibly decomposed by harsh conditions of high temperature and high pressure, most of the amino acids having a ring shape among the amino acids essential for the growth of plants There is a problem that the amino acid is not evenly contained because it is destroyed by this acid. On the other hand, since the decomposition process using audi and sugar according to the present invention is carried out under mild conditions, not high temperature and high pressure, the protein component in the blood of the livestock is not only a free amino acid, but also a double peptide type and a triple peptide type. Decomposed into amino acids in the form, the ineffective (肥效) can be maintained continuously during application. In addition, microelements such as potassium (K), which are useful for the growth and growth of crops, are chelated with the produced amino acids during enzymatic degradation, so they are very easy to absorb and move into plants during application. Will have

     The blood of the livestock ground in the blood milling step is transferred from the blood mill to the reactor through a pipe using a transfer pump. In the transfer process, the blood comes to the milled blood and sugar is added, or the ground blood is transferred to the reactor. After all have been transferred, audio and sugar can be added directly to the reactor. In this case, as the reactor, a conventionally known reactor may be used, but it is preferable to use a triple jacketed stirred tank configured as a triple jacket to control the temperature of the reactor using hot water as the reactor. Before transferring the pulverized blood from the blood grinder to the reactor, it is preferable to circulate the preheated hot water to adjust the temperature inside the reactor to 52 to 58 ° C, most preferably to fix the temperature of the reactor to 52 ° C. Let's do it. If the temperature inside the reactor is less than 52 ℃, the reaction time required for the decomposition of blood is long, so that decomposition does not proceed completely, odor may occur in the liquid product, the final product, and if it exceeds 58 ℃, the reaction time may be shortened. However, there is a disadvantage in that the energy cost required for maintaining the temperature is high and the decomposition of amino acids proceeds, thereby decreasing the amino acid content of the liquid fertilizer as a final product.

     When the temperature of the reactor is fixed at 52 ~ 58 ℃ transfer the blood of the livestock to the reactor through a pipe using a transfer pump from the blood mill proceeds with decomposition while stirring for 3 to 5 hours in the reactor. In this case, if the reaction time is less than 3 hours, the decomposition does not proceed completely, and if it exceeds 5 hours, the decomposition rate is no longer improved and there is no economic benefit. The completion of proteolysis is managed by the pH of the decomposed blood, and the pH of the decomposed blood is measured within 3 to 5 hours after the reaction starts. Let's do it. After the decomposition reaction is completed, it is left in the reactor for about 3 hours and completely cooled to room temperature.

     The blood decomposition process in the reactor is carried out under anaerobic conditions in which the reactor is sealed, which keeps the reactor at a temperature most suitable for decomposition by sealing the reactor, and in the decomposition process, the pulverized blood contacts with air and decays. This is to prevent it.

     The blood of livestock completely decomposed through the blood grinding step and the enzyme addition step can be used as organic fertilizer because it is rich in amino acids by itself, and can mix additives such as urea, galley and phosphoric acid, or filter the blood in the enzyme addition step. Then, by further treating additives such as urea, a liquid complex fertilizer (fourth type compound fertilizer) containing urea, galley, phosphoric acid, and the like may be prepared.

Hereinafter, the present invention will be described in more detail with reference to Examples.

After collecting the blood of the pig generated in the slaughterhouse, it was completely ground for 20 minutes using a blood mill, and 100 kg of the ground pig blood was added with 2 kg of Audi, 2 kg of white sugar, and 4 kg in total. Next, the hot water is circulated in a triple jacket reactor, and the temperature inside the reactor is fixed at 52 ° C., and then the pig blood added with the liquid audio and sugar is injected into the reactor through a pipe using a transfer pump, and then the reactor is sealed. Decomposition was for 4 hours. After 4 hours, the pH of the decomposed pig blood was measured. The pH 7.0 was confirmed and the decomposition was terminated. Then, the liquid fertilizer was prepared by allowing the reactor to stand at room temperature for 3 hours to cool.

In Example 1, instead of adding 2kg of Audi, 2kg of white sugar, 4kg in total, except that 1.3kg of Audi, 1.3kg of white sugar, and 2.6kg in total, liquid fertilizer was prepared.

In Example 1, instead of adding 2kg of Audi, 2kg of white sugar, 4kg in total, liquid fertilizer was prepared in the same manner, except that 3.1kg of Audi, 3.1kg of white sugar, and 6.2kg of total sugar were added.

≪ Comparative Example 1 &

In Example 1, 3 kg of actinidine extracted from kiwi is added instead of 2 kg of Audi, 2 kg of white sugar, and 4 kg in total. In addition, the temperature inside the reactor was fixed at 52 ° C. for 4 hours to be decomposed, and the pH of the decomposed blood was measured at 7.0 ° C. instead of confirming that it became 7.0. A liquid fertilizer was prepared in the same manner except that 7.24 was confirmed.

Comparative Example 2

In Example 1, instead of adding 2 kg of Audi, 2 kg of white sugar, and 4 kg in total, 1,000 cc of protease extracted from soybean is added. In addition, the temperature inside the reactor was fixed at 52 ° C. to be decomposed for 4 hours, and the pH of the decomposed blood was measured at 7.0 ° C. instead of being confirmed to be 7.0. The liquid fertilizer was prepared in the same manner except that the result was 7.7.

Experimental Example 1 Analysis of Amino Acid Components of Liquid Fertilizer

Component analysis of 18 amino acids of the liquid fertilizers prepared in Examples 1 to 3 and Comparative Examples 1 and 2 was carried out, and the results are shown in the following [Table 1].

Amino Acid Component Analysis (Unit:%) Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Aspartic acid 1.76 1.68 1.74 1.76 2.11 Threonine 2.48 2.42 2.47 2.47 0.68 Serine 1.67 1.58 1.68 1.67 0.90 Glutamic acid 1.71 1.60 1.70 1.71 1.75 Glycine 1.77 1.71 1.76 1.76 0.85 Alanine 1.29 1.24 1.31 1.29 1.44 Balin 1.26 1.23 1.21 1.26 1.46 Isoleucine 2.15 2.09 2.17 2.15 0.19 Leucine 2.15 2.11 2.18 2.15 2.29 Tyrosine 1.18 1.14 1.19 1.18 0.32 Phenylalanine 2.81 2.73 2.80 2.80 1.16 Lee Sin 1.26 1.23 1.21 1.26 1.64 Histidine 1.13 1.11 1.12 1.13 1.28 Arginine 1.21 1.17 1.25 1.21 0.54 Proline 0.56 0.53 0.54 0.56 1.02 Methionine 2.15 2.13 2.14 2.15 0.13 Cystine 0.22 0.19 0.22 0.21 0.28 Tryptophan 0.12 0.12 0.14 0.12 0.19 Sum 26.93 26.01 26.83 26.84 19.23

As can be seen in Table 1, the liquid fertilizer prepared according to Example 1, which is a preferred embodiment of the present invention, is to use an expensive actinidine or protease by adding a low-cost sugar mixed with Audi. It can be seen that there is an advantage that the manufacturing cost is cheaper, and also in the content of 18 amino acids essential for plant growth, compared to Comparative Examples 1 and 2, which are examples of the conventional liquid fertilizer production.

Experimental Example 2 Analysis of Water Soluble Sulfur and Salinity of Liquid Fertilizers

The liquid fertilizers prepared in Examples 1 to 3 and Comparative Examples 1 and 2 were each analyzed for acidity (pH), water-soluble S, and salinity analysis according to the fertilizer quality test method, and the results are shown in the following [Table 2]. .

 Acidity, Salinity Analysis Test Results of Liquid Fertilizer Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 pH 7.0 7.0 7.0 7.24 7.70 Water Soluble S (%) 0.095 0.096 0.096 0.104 0.107 Cl (%) 0.12 0.13 0.14 0.16 0.32

Since the liquid fertilizer according to Example 1, which is a preferred embodiment of the present invention from Table 2, has an acidity of 7.0 than that of Comparative Examples 1 and 2, it is neutral and has a very low content of water-soluble S, thereby inhibiting acidification of the soil by sulfur (S). In addition, since the salt content is very low, it can be seen that there is an excellent effect, such as no damage caused by the series.

<Experiment 3> Inspection of harmful components of liquid fertilizer

The liquid fertilizers prepared in Example 1 and Comparative Examples 1 and 2 were analyzed for 8 types of harmful heavy metals such as lead (Pb) according to RDA Notice No. 2009-27. ].

 Hazardous Ingredient Test Results (Unit: mg / kg) standard Example 1 Comparative Example 1 Comparative Example 2 lead 75 or less Not detected Not detected Not detected cadmium 2.5 〃 Not detected Not detected Not detected Copper 150 〃 1.03 1.05 1.45 chrome 150 〃 Not detected Not detected 4.57 arsenic 25 〃 Not detected Not detected Not detected Mercury One 〃 Not detected Not detected Not detected zinc 450 〃 4.20 4.23 4.59 nickel 25 〃 Not detected Not detected 5.33

     It can be seen from the Table 3 that the liquid fertilizer according to Example 1, which is a preferred embodiment of the present invention, has a lower content of harmful heavy metals than Comparative Examples 1 and 2, and thus has an excellent effect.

Experimental Example 4 Antibiotic Test of Liquid Fertilizer

The liquid fertilizers prepared in Examples 1 to 3 and Comparative Examples 1 and 2 were tested for antibiotics, and the results are shown in the following [Table 4].

Antibiotic Detection Test Results Example 1 Comparative Example 1 Comparative Example 2 Remarks Tetracycline system Not detected Same as left Same as left Antibiotics should not be detected in accordance with the Enforcement Rules of the Environment-Friendly Agriculture Promotion Act Beta-lactam meter Not detected Same as left Same as left Sulfonamide Not detected Same as left Same as left Macrolide system Not detected Same as left Same as left Aminoglycosides Not detected Same as left Same as left

It can be seen from Table 4 that the liquid fertilizer according to Example 1, which is a preferred embodiment of the present invention, does not detect antibiotics in liquid fertilizers as in Comparative Examples 1 and 2, and thus has an excellent effect.

Claims (4)

Collecting blood from livestock such as cattle or pigs and then crushing them with a blood mill;
Mixing and adding Audi and sugar to the blood ground in the milling step;
Adding the mixed blood of Audi and sugar in the addition step in a reactor maintained at 52 ~ 58 ℃ react for 3 to 5 hours in a sealed condition;
After confirming that the pH of the blood reacted in the reaction step is 7.0 The method for producing a liquid fertilizer using livestock blood, comprising the step of cooling at room temperature for 3 hours.
The method according to claim 1,
Method for producing a liquid fertilizer using the blood of the livestock, characterized in that the addition of 3 to 6 parts by weight of Audi and sugar per 100 parts by weight of blood pulverized in the addition step.
The method according to claim 2,
Method for producing a liquid fertilizer using the blood of the livestock, characterized in that the mixing ratio of Audi and sugar in the addition step is 1: 1.
Liquid fertilizer using the blood of livestock, characterized in that produced by any one of the manufacturing method of claim 1.

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